##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937281_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1783941 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04981162493603 31.0 31.0 34.0 30.0 34.0 2 32.27388854227802 33.0 31.0 34.0 31.0 34.0 3 32.35782517471149 34.0 31.0 34.0 30.0 34.0 4 35.999342467043476 37.0 35.0 37.0 35.0 37.0 5 35.88415928553691 37.0 35.0 37.0 35.0 37.0 6 35.966973122990055 37.0 35.0 37.0 35.0 37.0 7 35.918726011678636 37.0 35.0 37.0 35.0 37.0 8 35.89965138981614 37.0 35.0 37.0 35.0 37.0 9 37.57829154663747 39.0 37.0 39.0 35.0 39.0 10-11 37.50031671451018 39.0 37.0 39.0 35.0 39.0 12-13 37.41809650655487 39.0 37.0 39.0 34.0 39.0 14-15 38.59852035465298 40.0 38.0 41.0 34.0 41.0 16-17 38.57604455528518 40.0 38.0 41.0 34.0 41.0 18-19 38.58254813359859 40.0 38.0 41.0 34.0 41.0 20-21 38.477303341310055 40.0 38.0 41.0 34.0 41.0 22-23 38.349500067547076 40.0 38.0 41.0 34.0 41.0 24-25 38.206260184613726 40.0 38.0 41.0 33.5 41.0 26-27 38.16446788318672 40.0 38.0 41.0 33.5 41.0 28-29 38.06993224551709 40.0 37.5 41.0 33.0 41.0 30-31 38.14529936808448 40.0 37.5 41.0 34.0 41.0 32-33 38.07399263764889 40.0 37.0 41.0 33.5 41.0 34-35 37.96330119662029 40.0 37.0 41.0 33.0 41.0 36-37 37.80364288953503 40.0 37.0 41.0 33.0 41.0 38-39 37.673326360008545 40.0 37.0 41.0 33.0 41.0 40-41 37.50678609886762 40.0 37.0 41.0 33.0 41.0 42-43 37.30258343745673 40.0 36.5 41.0 32.0 41.0 44-45 37.011927524508934 40.0 36.0 41.0 31.5 41.0 46-47 36.66326857222296 39.0 36.0 41.0 30.5 41.0 48-49 36.36163387690512 39.0 36.0 41.0 30.0 41.0 50-51 36.13749361666109 39.0 35.5 40.5 30.0 41.0 52-53 35.91064278471093 39.0 35.0 40.0 29.0 41.0 54-55 35.68184429866234 39.0 35.0 40.0 28.0 41.0 56-57 35.43527420469623 38.0 35.0 40.0 28.0 41.0 58-59 35.1787424584109 38.0 34.5 40.0 27.5 41.0 60-61 34.85207946899589 38.0 34.0 40.0 27.0 41.0 62-63 34.476884605488635 37.5 34.0 40.0 26.0 41.0 64-65 34.12101297072044 37.0 33.5 39.5 26.0 41.0 66-67 33.6855913396239 36.0 33.0 39.0 25.5 40.5 68-69 33.289636540670344 36.0 32.5 39.0 24.5 40.0 70-71 32.88192462643103 35.0 32.0 38.5 23.5 40.0 72-73 32.419976893854674 35.0 32.0 37.5 22.5 40.0 74-75 31.965986823555262 35.0 31.0 37.0 21.5 39.0 76-77 30.647458912598566 33.5 29.5 35.5 20.5 38.5 78-79 31.308821311915587 34.0 31.0 36.0 21.0 38.5 80-81 31.172524763991635 34.0 31.0 36.0 20.0 37.5 82-83 30.83288460773086 34.0 31.0 35.0 19.5 37.0 84-85 30.448838834916625 34.0 31.0 35.0 18.0 36.5 86-87 30.047252403526798 34.0 30.0 35.0 16.0 36.0 88-89 29.627850080243682 34.0 30.0 35.0 9.0 36.0 90-91 29.15671118047065 34.0 29.0 35.0 4.5 35.0 92-93 28.758493694578465 34.0 29.0 35.0 2.0 35.0 94-95 28.300985290432813 33.0 29.0 35.0 2.0 35.0 96-97 27.76470438203954 33.0 27.5 35.0 2.0 35.0 98-99 27.196324317900647 33.0 27.0 35.0 2.0 35.0 100-101 25.158518975683613 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13335661165480417 1101 2 0.12221919608623466 1101 3 0.08394512572946411 1101 4 0.11363845896570268 1101 5 0.00865367691979202 1101 6 -0.058642365679290265 1101 7 -0.01963578787864151 1101 8 -0.011617756959637404 1101 9 0.048209702379281794 1101 10-11 0.06981195775686899 1101 12-13 0.021616397293492184 1101 14-15 0.10334526524620458 1101 16-17 0.01543539003370853 1101 18-19 -0.002777004048120091 1101 20-21 0.11294455333108999 1101 22-23 0.1299053609074292 1101 24-25 -0.004205458600459622 1101 26-27 -0.00781344574382814 1101 28-29 -0.04854005899861136 1101 30-31 0.08079611056262337 1101 32-33 0.09429751210073789 1101 34-35 0.01768450762353524 1101 36-37 -0.04430114543142594 1101 38-39 -0.18859001969864408 1101 40-41 -0.20502228716684812 1101 42-43 -0.08644248975233637 1101 44-45 -0.025811360228068736 1101 46-47 -0.1627952573468434 1101 48-49 -0.28149304302732503 1101 50-51 -0.21702071554527436 1101 52-53 -0.26938885885022046 1101 54-55 -0.30131461768747414 1101 56-57 -0.32553128911818874 1101 58-59 -0.39107791143307935 1101 60-61 -0.36897952810439705 1101 62-63 -0.2451877295185554 1101 64-65 -0.21109204527094505 1101 66-67 -0.1662983897187118 1101 68-69 -0.15655588843215185 1101 70-71 -0.39869653467442134 1101 72-73 -0.36567892128284285 1101 74-75 -0.3525813890986207 1101 76-77 -0.4299948616017275 1101 78-79 -0.29594636842631417 1101 80-81 -0.2515174570798919 1101 82-83 -0.5092438329754252 1101 84-85 -0.46905418682547406 1101 86-87 -0.37382498230817873 1101 88-89 -0.45771320078621613 1101 90-91 -0.45682511423538585 1101 92-93 -0.4415422891301688 1101 94-95 -0.4177805751616184 1101 96-97 -0.2741618779654651 1101 98-99 -0.2314738891251551 1101 100-101 0.27919412211371153 1102 1 0.1893083652422547 1102 2 0.21156995886700258 1102 3 0.19013696262697977 1102 4 0.14597899604807196 1102 5 0.03612866155559402 1102 6 0.16957129991850906 1102 7 0.154473126472098 1102 8 0.22399351593330863 1102 9 0.217558641552678 1102 10-11 0.19502734888315132 1102 12-13 0.24176165002057814 1102 14-15 0.16955571013741633 1102 16-17 0.1251250738447851 1102 18-19 0.19401332699360552 1102 20-21 0.19219679032447345 1102 22-23 0.1756075712215477 1102 24-25 0.28480841671218826 1102 26-27 0.13465693038419602 1102 28-29 0.17537673171634793 1102 30-31 0.17098264340209823 1102 32-33 0.0745149712817792 1102 34-35 0.17303965965930246 1102 36-37 0.1307965060442129 1102 38-39 -0.1226342199823236 1102 40-41 0.1215676480200969 1102 42-43 -0.008640740468962349 1102 44-45 0.09526357233894345 1102 46-47 0.16361456661805818 1102 48-49 0.0375502169587989 1102 50-51 0.132398354528938 1102 52-53 0.2926794778368986 1102 54-55 0.19898816712295542 1102 56-57 0.2835415774936294 1102 58-59 0.17423017773531768 1102 60-61 0.2696453759537576 1102 62-63 0.19367583315639791 1102 64-65 0.18265613074675713 1102 66-67 0.3313292710459237 1102 68-69 0.37488889150672833 1102 70-71 0.18333715347251456 1102 72-73 0.18826956360835823 1102 74-75 0.33132266635823626 1102 76-77 0.360533237698494 1102 78-79 0.20881015749365872 1102 80-81 0.375063390063719 1102 82-83 0.47224025350075394 1102 84-85 0.40094195300562063 1102 86-87 0.23046226464754938 1102 88-89 0.25312250628605426 1102 90-91 0.39373161090168907 1102 92-93 0.46694141432848113 1102 94-95 0.2897537426997481 1102 96-97 0.2550080417508198 1102 98-99 0.35179958209648987 1102 100-101 0.5534400769596317 1103 1 0.23354338373186323 1103 2 0.2931616172232623 1103 3 0.2393048188616902 1103 4 0.2042461205612014 1103 5 0.12426994158916216 1103 6 0.11696395766027479 1103 7 0.22743165117233843 1103 8 0.2098789239613268 1103 9 0.18176122348155133 1103 10-11 0.1869478799027675 1103 12-13 0.19663590197242087 1103 14-15 0.24013297232197317 1103 16-17 0.3135195679373908 1103 18-19 0.2653092257507055 1103 20-21 0.28533366270060867 1103 22-23 0.2897596675959946 1103 24-25 0.2960259122785658 1103 26-27 0.39492205446471473 1103 28-29 0.228815016056096 1103 30-31 0.3010798967090267 1103 32-33 0.3503699396211175 1103 34-35 0.2487079071088445 1103 36-37 0.20176661497610127 1103 38-39 0.19392978926203597 1103 40-41 0.2576485447788457 1103 42-43 0.26340563206309753 1103 44-45 0.13216223716441533 1103 46-47 0.19377712624604726 1103 48-49 0.2801091981208472 1103 50-51 0.2599458128787262 1103 52-53 0.34760710828560093 1103 54-55 0.32583983572764197 1103 56-57 0.22093800644548622 1103 58-59 0.40463301477721103 1103 60-61 0.30080384956018236 1103 62-63 0.26518951855440065 1103 64-65 0.300074730022601 1103 66-67 0.33550935033331797 1103 68-69 0.4472605197823185 1103 70-71 0.3885816278782883 1103 72-73 0.4833446480748549 1103 74-75 0.33618800211313626 1103 76-77 0.3071011843116871 1103 78-79 0.3920836984047611 1103 80-81 0.4096009687925495 1103 82-83 0.23615552915662974 1103 84-85 0.302372283088868 1103 86-87 0.40465819725949714 1103 88-89 0.3307254646238249 1103 90-91 0.42474098670559357 1103 92-93 0.47346255164948303 1103 94-95 0.45743180182318 1103 96-97 0.36999834965187617 1103 98-99 0.7322344195324106 1103 100-101 1.1608314410368834 1104 1 0.14197278237151068 1104 2 0.15986970243903897 1104 3 0.03885381253474662 1104 4 0.0804268646922992 1104 5 0.0413784533944721 1104 6 0.18226332308779547 1104 7 0.11313523777021572 1104 8 0.0970099538011695 1104 9 0.10349660003866745 1104 10-11 0.06148591976846518 1104 12-13 0.009543404882926154 1104 14-15 0.13118198756515653 1104 16-17 0.11149364916020943 1104 18-19 0.052545641048645564 1104 20-21 0.05196254007694989 1104 22-23 0.06559928096547907 1104 24-25 0.11301721174201163 1104 26-27 -0.04358372588804116 1104 28-29 0.009445251029568169 1104 30-31 0.13610792759744328 1104 32-33 -0.013717449017619288 1104 34-35 0.022208115913500137 1104 36-37 -0.11255130985480122 1104 38-39 -0.14527198705960132 1104 40-41 -0.09197761710976948 1104 42-43 0.024134420488366004 1104 44-45 -0.08055982138247231 1104 46-47 -0.26754162705366014 1104 48-49 -0.23538211205428894 1104 50-51 -0.2511918541026361 1104 52-53 -0.4449021015699799 1104 54-55 -0.33425684537877487 1104 56-57 -0.27828370830005866 1104 58-59 -0.3189364678714597 1104 60-61 -0.37142815340035185 1104 62-63 -0.4106197499073545 1104 64-65 -0.3654561547251447 1104 66-67 -0.29023727673040867 1104 68-69 -0.3653768430162927 1104 70-71 -0.3013794020994922 1104 72-73 -0.31284791707058446 1104 74-75 -0.2447987121584454 1104 76-77 -0.11274629290835492 1104 78-79 -0.16867273499448032 1104 80-81 -0.13077507328650384 1104 82-83 -0.14241920515668127 1104 84-85 -0.059612532131481544 1104 86-87 -0.10860302632113772 1104 88-89 -0.08326873227941078 1104 90-91 -0.22200657623734088 1104 92-93 -0.27519166343135026 1104 94-95 -0.13481231562276363 1104 96-97 -0.045538771355925434 1104 98-99 0.05840564052756747 1104 100-101 0.42304255179256955 1105 1 0.013526121697530868 1105 2 0.09271743189538029 1105 3 0.04809658301419972 1105 4 0.04227343841553477 1105 5 -0.04189312222404595 1105 6 0.03492545449480389 1105 7 0.020131058874490293 1105 8 -0.07982185222132898 1105 9 0.011014434996269529 1105 10-11 0.043884584254662684 1105 12-13 0.035439098036555094 1105 14-15 0.07669636615361242 1105 16-17 0.05068089184713642 1105 18-19 0.09979965323721984 1105 20-21 0.2137370158583849 1105 22-23 0.08720978808012347 1105 24-25 0.08034059885999767 1105 26-27 0.0675038854885841 1105 28-29 0.0026216295159073866 1105 30-31 -0.049347163584791076 1105 32-33 -0.05266735641972531 1105 34-35 -0.03140970001395971 1105 36-37 -0.1744665777543588 1105 38-39 -0.19306797154462174 1105 40-41 -0.1066175219281078 1105 42-43 -0.05361299845173306 1105 44-45 -0.006467272998726514 1105 46-47 0.0390695685499054 1105 48-49 0.07474563745332574 1105 50-51 -0.06637589725000481 1105 52-53 -0.10104442847689654 1105 54-55 -0.22143408718383029 1105 56-57 -0.09993444173416322 1105 58-59 -0.22326404626249996 1105 60-61 -0.06851159052274625 1105 62-63 -0.06449981049437525 1105 64-65 -0.0820094399003537 1105 66-67 -0.11529780960537295 1105 68-69 0.023745031901512448 1105 70-71 -0.18332753180771277 1105 72-73 -0.42784599084874486 1105 74-75 -0.22314194477642246 1105 76-77 -0.2539018403498865 1105 78-79 -0.18161833818040307 1105 80-81 -0.21525529508824093 1105 82-83 -0.03219862568107956 1105 84-85 -0.08739623211739911 1105 86-87 -0.020101100872885524 1105 88-89 0.06904002638278683 1105 90-91 -0.009889789948946515 1105 92-93 0.0193465306853966 1105 94-95 0.05614186814653621 1105 96-97 0.06693030466098904 1105 98-99 0.2837938631895298 1105 100-101 0.6052775261018759 1106 1 -0.06771090542553537 1106 2 0.05607854033119963 1106 3 -0.023375873803765046 1106 4 0.05505651696848446 1106 5 -0.03682851106994178 1106 6 -0.04760645255546336 1106 7 0.03833642636600132 1106 8 0.005447933803004901 1106 9 0.01662071046462188 1106 10-11 -0.2149519168130567 1106 12-13 -0.14173428435984903 1106 14-15 -0.020287782443745073 1106 16-17 0.11679299743016713 1106 18-19 0.06829146185138057 1106 20-21 0.09557415789001311 1106 22-23 0.15933101328428734 1106 24-25 0.17476205421337454 1106 26-27 0.07970843600018895 1106 28-29 0.19871075338799926 1106 30-31 0.19933371277559786 1106 32-33 0.12787440752622103 1106 34-35 0.17862636665957865 1106 36-37 0.12639540245879033 1106 38-39 0.24576331546440144 1106 40-41 0.18114106383319495 1106 42-43 -0.03424708776822882 1106 44-45 0.05724852427070459 1106 46-47 0.4052451063119804 1106 48-49 0.30162128713499925 1106 50-51 0.1508519172623437 1106 52-53 0.34539772370055744 1106 54-55 0.2354483042425315 1106 56-57 0.32460899602425997 1106 58-59 0.06055061885876967 1106 60-61 0.27197806604679897 1106 62-63 0.33615377342373876 1106 64-65 0.25584861150295524 1106 66-67 0.19443819961387732 1106 68-69 0.2625585512582944 1106 70-71 0.2861121063299734 1106 72-73 0.3896863645441542 1106 74-75 0.18755700477341364 1106 76-77 0.3170208155654173 1106 78-79 0.22311184258470007 1106 80-81 0.30010658383268307 1106 82-83 0.31643293299036657 1106 84-85 0.1090721564063557 1106 86-87 0.14603956579599497 1106 88-89 0.08021504938110269 1106 90-91 1.0777619607438282E-5 1106 92-93 0.23910714893327523 1106 94-95 -0.1687765168901123 1106 96-97 0.04865187392660175 1106 98-99 0.33630873321997967 1106 100-101 0.7050249597554696 1107 1 0.12139675177994036 1107 2 0.07893260192144425 1107 3 0.04444323322965715 1107 4 0.10439064086021688 1107 5 0.06682938635767499 1107 6 0.07486118288822041 1107 7 0.09028563084517316 1107 8 0.09919694908834487 1107 9 0.10263348587122323 1107 10-11 0.10593869609906648 1107 12-13 0.1194824596156252 1107 14-15 0.07676505099431097 1107 16-17 0.03303758993996553 1107 18-19 0.20755342585309933 1107 20-21 0.12913516261085078 1107 22-23 0.21989988736235233 1107 24-25 0.28177505534069525 1107 26-27 0.2643798130058741 1107 28-29 0.1521225766518981 1107 30-31 0.22950520907242122 1107 32-33 0.12260485776509 1107 34-35 0.24621380449795538 1107 36-37 0.08107521666041606 1107 38-39 0.20096981133358582 1107 40-41 0.3253521635046468 1107 42-43 0.3293772473685195 1107 44-45 0.251511745491797 1107 46-47 0.254087306953906 1107 48-49 0.21701074539885923 1107 50-51 0.06223258206451732 1107 52-53 0.21993832416909953 1107 54-55 0.10577754137725748 1107 56-57 0.1299369373548771 1107 58-59 0.35448641642779677 1107 60-61 0.3491388595805134 1107 62-63 0.3509555218268119 1107 64-65 0.3610125998529341 1107 66-67 0.3809436378111002 1107 68-69 0.3986102650958685 1107 70-71 0.36594296370213186 1107 72-73 0.21957638518055234 1107 74-75 0.2176490808187843 1107 76-77 0.33873132199652645 1107 78-79 0.371946358433064 1107 80-81 0.343843348463853 1107 82-83 0.40802473990719434 1107 84-85 0.4955304569158656 1107 86-87 0.4300250026298862 1107 88-89 0.3282956194577409 1107 90-91 0.43429662878926933 1107 92-93 0.36101305490612745 1107 94-95 0.4461836540145505 1107 96-97 0.16566041208594484 1107 98-99 0.494902936892089 1107 100-101 0.8919180137953227 1108 1 0.033119677539410475 1108 2 -0.02046520208565994 1108 3 -0.08524731049398326 1108 4 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-0.6803566880509919 2316 60-61 -0.7406735730672409 2316 62-63 -0.7165636388938594 2316 64-65 -0.9542109097445817 2316 66-67 -0.7745194162096212 2316 68-69 -0.4428765651834823 2316 70-71 -0.562547516612149 2316 72-73 -0.5265591279560553 2316 74-75 -0.43198903902237973 2316 76-77 -0.471972568449857 2316 78-79 -0.6678258122095713 2316 80-81 -0.5888194293380096 2316 82-83 -0.7874965344407379 2316 84-85 -0.8625255794663786 2316 86-87 -0.75967389764903 2316 88-89 -0.7320610346621095 2316 90-91 -0.5727590358649195 2316 92-93 -0.448226110962068 2316 94-95 -0.4731083491217518 2316 96-97 -0.366353258221654 2316 98-99 -0.6711724580220277 2316 100-101 -1.006777558681236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 9.0 5 38.0 6 69.0 7 168.0 8 394.0 9 843.0 10 1409.0 11 2256.0 12 3542.0 13 5028.0 14 6824.0 15 9211.0 16 12547.0 17 17095.0 18 15202.0 19 9155.0 20 8003.0 21 8302.0 22 8545.0 23 9731.0 24 11239.0 25 13569.0 26 16643.0 27 20560.0 28 26512.0 29 33416.0 30 41629.0 31 53211.0 32 67723.0 33 89313.0 34 121609.0 35 173646.0 36 264372.0 37 396207.0 38 310028.0 39 25882.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.080685073799785 20.156853991066402 15.913786953176004 21.84867398195781 2 15.597488930407453 20.901924447052902 40.070719827617616 23.42986679492203 3 19.273955809076647 25.020670526659792 30.22482245769339 25.480551206570173 4 13.048133318310414 17.51240652017079 37.46015142877483 31.979308732743966 5 13.425086242614084 37.81415550637996 34.31130093612412 14.449457314881833 6 31.461298327691328 37.19276590425356 17.21222843132144 14.13370733673367 7 28.220383970097668 33.15356281401683 20.584088823565352 18.04196439232015 8 26.298571533475602 35.55846297607376 19.4064714023614 18.736494088089238 9 26.161795709611475 16.987613379590467 20.295850591471353 36.554740319326704 10-11 24.697817921108378 27.315505389471962 27.2663445708126 20.72033211860706 12-13 25.822868186092528 24.419816721532488 28.132642150123537 21.624672942251447 14-15 22.531742921991256 25.779047625453984 26.628515180715056 25.060694271839708 16-17 21.585663427209756 29.33356540378858 27.14621727960734 21.934553889394323 18-19 21.54194000810565 27.41214535682514 30.064895643970292 20.98101899109892 20-21 22.29314198171352 26.34672335015564 30.355067796524658 21.00506687160618 22-23 21.946830724385833 26.3343189876256 30.290286867441175 21.428563420547388 24-25 21.809518150564568 26.54804900840219 30.3763469490829 21.266085891950343 26-27 21.733713693760155 26.82006276059155 30.20244158657161 21.243781959076685 28-29 21.468362179074834 26.87543567117957 30.35648868719433 21.299713462551267 30-31 21.61753107305679 26.64322979291355 30.290491669847825 21.44874746418183 32-33 21.53019634617961 26.724370368750982 30.233623197179725 21.51181008788968 34-35 21.953220426011846 26.72445445224926 30.011110232905686 21.311214888833206 36-37 21.94576502249794 26.5352105254602 29.997348567020993 21.521675885020862 38-39 21.754643230914027 26.766776479715414 30.10388235933812 21.37469793003244 40-41 21.956247431949823 26.66206449652763 30.012819930703987 21.36886814081856 42-43 21.80049935849225 26.773845321086053 29.73013505441113 21.695520266010572 44-45 21.91854999688891 26.872161130889417 29.509552165682614 21.69973670653906 46-47 22.016479244549007 27.133941088858883 29.00530342651467 21.844276240077445 48-49 22.144258134097484 27.322428264163445 28.80961309819103 21.723700503548045 50-51 22.152890706587268 27.43187695108751 28.636260952576347 21.77897138974888 52-53 22.317133806555262 27.4925852368436 28.45085683887528 21.739424117725868 54-55 22.14176365698193 27.40138266904567 28.49584151045354 21.961012163518863 56-57 22.17321088533758 27.714986089786603 28.369267817713702 21.74253520716212 58-59 22.30757631558443 27.344318001548256 28.456966906416746 21.891138776450568 60-61 22.225707016095264 27.296810825021677 28.894957848942315 21.582524309940744 62-63 22.168754459928888 27.339777492641293 28.79248809237525 21.69897995505457 64-65 22.040639236387303 27.35098862574491 28.683207572447746 21.925164565420044 66-67 21.989880831260674 27.632584261475014 28.429611741643924 21.947923165620388 68-69 21.941729014580638 27.42685996902364 28.685786133061576 21.945624883334148 70-71 22.03189455256648 27.421955098290805 28.53628567312484 22.009864676017873 72-73 22.134273800027692 27.36346631074976 28.441251008303542 22.061008880919 74-75 22.09700320806574 27.649148710635608 28.1515195850087 22.102328496289957 76-77 22.14846230901134 27.40816540457336 28.411029288524674 22.032342997890623 78-79 22.06345389225316 27.420805957147685 28.407245531102205 22.108494619496945 80-81 21.99330022685728 27.73166265027823 28.28067183836237 21.99436528450212 82-83 22.048826234760895 27.61569415089141 28.2573922811801 22.078087333167595 84-85 22.053759893627127 27.522074360668274 28.1602779506966 22.263887795007996 86-87 22.076066416994735 27.384818219885076 28.283586732969308 22.255528630150884 88-89 22.131365331028324 27.375456923743553 28.201240960323236 22.29193678490488 90-91 22.023763473050533 27.418701990081544 28.325277253600007 22.23225728326791 92-93 22.05736029198668 27.249109683914668 28.35330903848105 22.340220985617602 94-95 21.89108272078505 27.204991644903053 28.337512283197707 22.56641335111419 96-97 21.905489026823197 27.228843330581 28.29493800523672 22.570729637359083 98-99 21.814186575781697 27.189115104768096 28.147275132571725 22.849423186878482 100-101 21.54736352584251 27.21869755454748 28.508685744816177 22.72525317479383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 326.0 1 225.0 2 188.0 3 274.5 4 244.5 5 228.0 6 447.5 7 1188.5 8 2098.5 9 2545.5 10 2399.0 11 2076.0 12 2068.5 13 2278.0 14 2462.0 15 2653.0 16 2808.0 17 2927.5 18 3123.5 19 3290.0 20 3551.0 21 4050.5 22 4754.5 23 5624.5 24 6854.5 25 8492.0 26 10615.0 27 13164.0 28 15659.5 29 18600.5 30 22298.0 31 26204.0 32 30154.5 33 33748.0 34 37839.0 35 41355.5 36 44923.0 37 48807.0 38 51593.0 39 53635.0 40 55488.0 41 58840.0 42 62067.0 43 65028.5 44 67582.0 45 68909.0 46 68417.0 47 68548.0 48 71948.5 49 71024.0 50 66934.5 51 63702.5 52 59564.5 53 57219.5 54 55239.5 55 50850.0 56 45348.0 57 39582.5 58 34690.5 59 30488.0 60 25660.0 61 20484.0 62 15891.5 63 12033.0 64 8871.5 65 6718.5 66 4870.5 67 3691.0 68 2642.0 69 1623.0 70 1142.0 71 845.0 72 630.0 73 526.5 74 416.0 75 305.5 76 217.0 77 121.5 78 66.5 79 42.5 80 26.0 81 13.5 82 10.5 83 6.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05656016650774885 2 0.0 3 0.0 4 0.0 5 0.017377256310606683 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0024384214500367445 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.968906482893773E-4 24-25 1.1211133103617216E-4 26-27 0.0010650576448436356 28-29 0.0031110894362537775 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014154055543316734 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.2422266207234432E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.401391637952152E-4 84-85 0.0015415308017473672 86-87 0.0 88-89 0.0 90-91 0.010930854776026785 92-93 0.0013453359724340658 94-95 0.0 96-97 0.0 98-99 5.605566551808608E-5 100-101 0.013593498888135873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1783941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.391035280906756 #Duplication Level Percentage of deduplicated Percentage of total 1 76.787290878514 44.06902119934047 2 13.354467936893395 15.328534810479736 3 4.333489975509819 7.461104282218193 4 1.8764732987024677 4.307709811580512 5 1.0054349478218085 2.885147628154903 6 0.5889610693367202 2.028065130563057 7 0.3880269945558696 1.5588489654150088 8 0.2703039487963883 1.2410418769553546 9 0.19537645623554137 1.009157138358524 >10 1.0839748677542118 11.321982210173678 >50 0.07731762435897682 3.0616905955559575 >100 0.03711937055479248 3.921835747614267 >500 0.001175087310709626 0.40418726732711135 >1k 2.937718276774065E-4 0.22715863939602116 >5k 2.937718276774065E-4 1.1745146968672553 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8686 0.4868995106900957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7065 0.3960332768852781 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5148 0.28857456608710713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 5.605566551808608E-5 18-19 0.0 0.0 0.0 0.0 5.605566551808608E-5 20-21 0.0 0.0 0.0 5.605566551808608E-5 5.605566551808608E-5 22-23 0.0 0.0 0.0 2.2422266207234432E-4 5.605566551808608E-5 24-25 0.0 0.0 0.0 2.802783275904304E-4 8.408349827712913E-5 26-27 0.0 0.0 0.0 5.325288224218177E-4 1.1211133103617216E-4 28-29 5.605566551808608E-5 0.0 0.0 0.0010090019793255495 1.1211133103617216E-4 30-31 5.605566551808608E-5 0.0 0.0 0.0023823657845186584 1.1211133103617216E-4 32-33 5.605566551808608E-5 0.0 0.0 0.003979952251784111 1.1211133103617216E-4 34-35 5.605566551808608E-5 0.0 0.0 0.006250206705266598 1.1211133103617216E-4 36-37 5.605566551808608E-5 0.0 0.0 0.009921852796701235 1.1211133103617216E-4 38-39 1.6816699655425825E-4 0.0 0.0 0.015807697676100275 1.1211133103617216E-4 40-41 1.6816699655425825E-4 0.0 0.0 0.0215534033917041 1.1211133103617216E-4 42-43 1.9619482931330129E-4 0.0 0.0 0.027327136940066964 1.1211133103617216E-4 44-45 2.802783275904304E-4 0.0 0.0 0.03206384067634524 1.1211133103617216E-4 46-47 2.802783275904304E-4 0.0 0.0 0.03960332768852781 1.1211133103617216E-4 48-49 2.802783275904304E-4 0.0 0.0 0.04652620238001144 1.1211133103617216E-4 50-51 2.802783275904304E-4 0.0 0.0 0.0533369657404589 1.1211133103617216E-4 52-53 2.802783275904304E-4 0.0 0.0 0.06861213459413737 1.1211133103617216E-4 54-55 2.802783275904304E-4 0.0 0.0 0.08991328749101007 1.1211133103617216E-4 56-57 2.802783275904304E-4 0.0 0.0 0.1224816291570181 1.1211133103617216E-4 58-59 2.802783275904304E-4 0.0 0.0 0.16777460689563164 1.1211133103617216E-4 60-61 2.802783275904304E-4 0.0 0.0 0.2239423837447539 1.1211133103617216E-4 62-63 2.802783275904304E-4 0.0 0.0 0.2659000493850413 1.1211133103617216E-4 64-65 2.802783275904304E-4 0.0 0.0 0.321703464408296 1.1211133103617216E-4 66-67 2.802783275904304E-4 0.0 0.0 0.4062354080095698 1.1211133103617216E-4 68-69 2.802783275904304E-4 0.0 0.0 0.5110595025283908 1.1211133103617216E-4 70-71 3.0830616034947344E-4 0.0 0.0 0.6280476764646364 1.1211133103617216E-4 72-73 3.363339931085165E-4 0.0 0.0 0.7492400253147385 1.1211133103617216E-4 74-75 3.363339931085165E-4 0.0 0.0 0.879036918821867 1.1211133103617216E-4 76-77 3.363339931085165E-4 0.0 0.0 1.0163172436756596 1.1211133103617216E-4 78-79 3.363339931085165E-4 0.0 0.0 1.1542982643484285 1.1211133103617216E-4 80-81 4.4844532414468864E-4 0.0 0.0 1.299314271043717 1.1211133103617216E-4 82-83 4.4844532414468864E-4 0.0 0.0 1.481607295308533 1.1211133103617216E-4 84-85 4.4844532414468864E-4 0.0 0.0 1.717825869801748 1.1211133103617216E-4 86-87 4.764731569037317E-4 0.0 0.0 2.000626702340492 1.1211133103617216E-4 88-89 5.045009896627748E-4 0.0 0.0 2.324880699529861 1.1211133103617216E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 38.541187 1 GTATCAA 9075 0.0 36.143814 1 ATCAACG 11280 0.0 28.722795 3 TATCAAC 11545 0.0 28.310392 2 TCAACGC 11480 0.0 28.26378 4 CAACGCA 11655 0.0 27.96168 5 AACGCAG 12015 0.0 27.118553 6 ACGCAGA 13540 0.0 23.959646 7 CGCAGAG 13600 0.0 23.853941 8 GAGTACT 8115 0.0 22.359043 12-13 GCAGAGT 14580 0.0 21.98997 9 GTACATG 7240 0.0 21.470427 1 AGTACTT 8460 0.0 21.22266 12-13 GTACTTT 8515 0.0 21.029207 14-15 AGAGTAC 12610 0.0 20.340284 10-11 TACATGG 7650 0.0 20.120325 2 ACATGGG 7490 0.0 19.598736 3 TGCACCG 340 4.1836756E-11 19.561428 5 CGCGGAA 245 0.0 19.387075 82-83 TCGCGGA 275 0.0 18.999332 82-83 >>END_MODULE