FastQCFastQC Report
Thu 26 May 2016
SRR937280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834651
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69290.3776740099343145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48740.2656636057757034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36170.19714921257503473No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26840.14629485389864338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83650.036.8674621
GGTATCA51450.036.7588771
ATCAACG105950.028.9223
TATCAAC106650.028.8204752
TCAACGC106950.028.7412034
CAACGCA108450.028.2107095
AACGCAG110900.027.5852186
GTACATG78900.025.9576151
ACGCAGA123100.024.5825657
TACATGG82600.024.3861662
CGCAGAG124700.024.1528648
ACATGGG80450.023.5031783
GAGTACT64950.022.16050312-13
GCAGAGT132100.022.1507329
AGTACTT68900.021.3381912-13
AGAGTAC111700.020.88010210-11
CATGGGG37400.020.7060594
GTACTTT69650.020.42528514-15
GTGTAGC10150.020.1310731
CGCGCGT1200.004761244619.7940467
Produced by FastQC (version 0.11.5)