##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937280_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1834651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.695199250429646 33.0 31.0 34.0 30.0 34.0 2 31.799421252325374 33.0 31.0 34.0 30.0 34.0 3 31.84538040204922 33.0 31.0 34.0 30.0 34.0 4 35.459797530974555 37.0 35.0 37.0 33.0 37.0 5 35.47508926765908 37.0 35.0 37.0 33.0 37.0 6 35.50165726342503 37.0 35.0 37.0 33.0 37.0 7 35.392569485967634 37.0 35.0 37.0 33.0 37.0 8 35.39703464037574 37.0 35.0 37.0 33.0 37.0 9 37.0159071125789 39.0 37.0 39.0 33.0 39.0 10-11 36.90532640812884 39.0 37.0 39.0 33.0 39.0 12-13 36.75657986178298 39.0 37.0 39.0 33.0 39.0 14-15 37.92010796603823 40.0 38.0 41.0 33.0 41.0 16-17 37.848615894794165 40.0 38.0 41.0 33.0 41.0 18-19 37.807300680074846 40.0 38.0 41.0 33.0 41.0 20-21 37.692994743959474 40.0 37.5 41.0 32.0 41.0 22-23 37.53342897368491 40.0 37.0 41.0 32.0 41.0 24-25 37.47105880082915 40.0 37.0 41.0 32.0 41.0 26-27 37.51561659410972 40.0 37.0 41.0 32.0 41.0 28-29 37.42806152232768 40.0 37.0 41.0 32.0 41.0 30-31 37.225500653802825 39.5 37.0 41.0 31.5 41.0 32-33 37.00218461167819 39.0 36.0 41.0 31.0 41.0 34-35 37.021920790384655 39.5 36.0 41.0 31.0 41.0 36-37 36.884182332225585 39.5 36.0 41.0 30.5 41.0 38-39 36.77339450391382 39.0 36.0 41.0 30.0 41.0 40-41 36.558846886955614 39.0 35.5 41.0 30.0 41.0 42-43 36.292113595446764 39.0 35.0 41.0 30.0 41.0 44-45 36.06539663401922 39.0 35.0 41.0 29.5 41.0 46-47 35.744542967572585 39.0 35.0 40.5 27.5 41.0 48-49 35.43358001058512 39.0 35.0 40.0 27.0 41.0 50-51 34.47509335563004 38.0 34.0 39.5 25.0 40.5 52-53 34.605992638381906 38.0 34.0 39.5 26.0 40.5 54-55 34.98061020869909 38.0 35.0 40.0 26.0 41.0 56-57 34.76594921867974 38.0 34.0 40.0 25.5 41.0 58-59 34.48472325254231 38.0 34.0 40.0 24.5 41.0 60-61 34.16380581374877 38.0 34.0 40.0 23.0 41.0 62-63 33.84148620091777 37.5 33.0 40.0 22.5 41.0 64-65 33.423416497197564 37.0 33.0 40.0 22.0 41.0 66-67 33.06283129597945 36.0 32.5 39.0 20.5 41.0 68-69 32.573365724598304 36.0 32.0 39.0 19.0 40.0 70-71 32.09433810572147 35.0 31.0 38.5 17.5 40.0 72-73 31.615728550007603 35.0 31.0 38.0 13.5 40.0 74-75 31.114190110271654 35.0 30.5 37.0 9.5 39.0 76-77 30.614638424419685 34.5 30.0 36.5 7.0 39.0 78-79 30.098560162123476 34.0 29.5 36.0 4.5 38.5 80-81 29.587530543956316 34.0 29.0 35.5 2.0 37.5 82-83 29.088558259854324 34.0 29.0 35.0 2.0 37.0 84-85 28.545216501667074 34.0 28.5 35.0 2.0 36.5 86-87 28.053786523976495 33.5 27.0 35.0 2.0 36.0 88-89 27.559008225542623 33.0 26.0 35.0 2.0 35.5 90-91 27.019206105139343 33.0 25.5 35.0 2.0 35.0 92-93 26.470237118667256 32.0 25.0 35.0 2.0 35.0 94-95 25.881747809256368 32.0 23.5 34.5 2.0 35.0 96-97 25.086983300911182 32.0 19.0 34.0 2.0 35.0 98-99 24.102064370825843 31.5 4.5 34.0 2.0 35.0 100-101 21.455178123795754 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011465321954265306 1101 2 0.0028588804534912526 1101 3 0.037638414703852874 1101 4 0.04705303872337652 1101 5 -0.07921493847919692 1101 6 -0.08841138867092724 1101 7 -0.11428849309919542 1101 8 -0.23764435099504766 1101 9 -0.11513994727614829 1101 10-11 -0.09607868320006929 1101 12-13 -0.22077072882712656 1101 14-15 -0.3577572858298268 1101 16-17 -0.27114372952797083 1101 18-19 -0.11777519381816859 1101 20-21 0.039404463315989346 1101 22-23 -0.13632730513332803 1101 24-25 -0.1599028521629009 1101 26-27 -0.1858027467474912 1101 28-29 -0.376859758599565 1101 30-31 -0.27607677420666477 1101 32-33 -0.5178112954814083 1101 34-35 -0.4085473753714979 1101 36-37 -0.2276333242403723 1101 38-39 -0.3321961143821497 1101 40-41 -0.35962370176824265 1101 42-43 -0.2763280821949863 1101 44-45 -0.22013990411905837 1101 46-47 -0.3101402743630217 1101 48-49 -0.3307205434286047 1101 50-51 -0.3179481049068116 1101 52-53 -0.2712401515632976 1101 54-55 -0.2607978192852798 1101 56-57 -0.44790016139881317 1101 58-59 -0.4819806485011213 1101 60-61 -0.3390480781601397 1101 62-63 -0.48759704589918584 1101 64-65 -0.43618120410693706 1101 66-67 -0.5876033627043924 1101 68-69 -0.44039051677839325 1101 70-71 -0.41340958279630513 1101 72-73 -0.4401146108042191 1101 74-75 -0.3404825547200083 1101 76-77 -0.3273926451048723 1101 78-79 -0.609479178142724 1101 80-81 -0.4155309166666896 1101 82-83 -0.16405625397212376 1101 84-85 -0.21143766292519928 1101 86-87 -0.2578736509686088 1101 88-89 -0.35684886816410355 1101 90-91 -0.30373766305820027 1101 92-93 -0.039769315378748615 1101 94-95 0.06497348027926719 1101 96-97 0.30597957751296434 1101 98-99 0.31223482160747196 1101 100-101 0.44957178298764333 1102 1 0.17098036078652612 1102 2 0.043804906158573687 1102 3 0.06154763130960106 1102 4 0.017013946454405016 1102 5 0.05838989976155773 1102 6 -0.018706790788193928 1102 7 -0.004232203730985873 1102 8 -0.015303147578883625 1102 9 0.07911497630687592 1102 10-11 0.09079684777709218 1102 12-13 0.1304052810650731 1102 14-15 0.20287715437165588 1102 16-17 0.19201166545728654 1102 18-19 0.13990921316749194 1102 20-21 0.09121394086276524 1102 22-23 0.13227882932205404 1102 24-25 0.13072336512877314 1102 26-27 0.06442611052018066 1102 28-29 0.1347386351028632 1102 30-31 0.05263494590976592 1102 32-33 -0.09136182752631328 1102 34-35 0.13227592417655387 1102 36-37 -0.06387392102396916 1102 38-39 -0.03861314866983179 1102 40-41 -0.20336551680834702 1102 42-43 -0.20960171624256674 1102 44-45 -0.17580257133666777 1102 46-47 -0.04490177653119076 1102 48-49 -0.2934921940465358 1102 50-51 -0.31853665015673727 1102 52-53 -0.2032947572418422 1102 54-55 -0.10494859287172886 1102 56-57 -0.12459598217437673 1102 58-59 -0.20124520750255925 1102 60-61 -0.08901038553539564 1102 62-63 -0.2906026532238499 1102 64-65 -0.27232634721284654 1102 66-67 -0.43550255847500097 1102 68-69 -0.5038575641620042 1102 70-71 -0.556946957156363 1102 72-73 -0.6693185734440057 1102 74-75 -0.641853357009456 1102 76-77 -0.542185032069451 1102 78-79 -0.5641525747653624 1102 80-81 -0.6200610041290169 1102 82-83 -0.6914244442784572 1102 84-85 -0.5071949656461179 1102 86-87 -0.4718487640593487 1102 88-89 -0.4489193433381722 1102 90-91 -0.38592906852226605 1102 92-93 -0.16125972035448655 1102 94-95 -0.26343271088228803 1102 96-97 -0.12998232540627086 1102 98-99 -0.2658606948483815 1102 100-101 -0.05495094605100803 1103 1 0.03821728746027375 1103 2 -0.02855885457152496 1103 3 -0.09261051604597981 1103 4 -0.0837978719786534 1103 5 -0.2572567966869599 1103 6 -0.18276251581819025 1103 7 -0.10727395514958005 1103 8 0.004587833475156344 1103 9 -0.03920753383953013 1103 10-11 -0.06994071367576993 1103 12-13 -0.01849462925054013 1103 14-15 -0.07865770871405431 1103 16-17 -0.07334903626217937 1103 18-19 -0.1377791214029216 1103 20-21 -0.10744386395563055 1103 22-23 -0.06850275901283709 1103 24-25 -0.17067494897411706 1103 26-27 -0.2734444728942407 1103 28-29 -0.2928353968722135 1103 30-31 -0.25070238280839874 1103 32-33 -0.17515293595972992 1103 34-35 -0.1388888505059782 1103 36-37 -0.18373467197984183 1103 38-39 -0.4597501815459921 1103 40-41 -0.23058769373542276 1103 42-43 -0.26644303628665966 1103 44-45 -0.24461681029527682 1103 46-47 -0.2901819634813094 1103 48-49 -0.3498771507873215 1103 50-51 -0.5228359948359795 1103 52-53 -0.39747628395181067 1103 54-55 -0.2294463020550097 1103 56-57 -0.05321642026196827 1103 58-59 -0.2509176000639428 1103 60-61 -0.22686122144261134 1103 62-63 -0.27616844312943556 1103 64-65 -0.3288838961077758 1103 66-67 -0.20382803339460764 1103 68-69 -0.2360347771269602 1103 70-71 -0.30918959631276266 1103 72-73 -0.22626551353335245 1103 74-75 -0.26234756352496547 1103 76-77 -0.24293459026530329 1103 78-79 -0.22982727773214862 1103 80-81 -0.35501213301935763 1103 82-83 -0.3571830609152826 1103 84-85 -0.4426372939248644 1103 86-87 -0.24765711476660712 1103 88-89 0.015361757397410969 1103 90-91 0.0835777096904593 1103 92-93 -0.05644175213473801 1103 94-95 -0.4135140303757794 1103 96-97 -0.4005559984320257 1103 98-99 -0.2452729920923069 1103 100-101 0.34440320987276607 1104 1 0.123087508813299 1104 2 0.07791009850371466 1104 3 0.1136882071214238 1104 4 0.09067587179068681 1104 5 0.004404547587867569 1104 6 0.03939455267552461 1104 7 0.06859454766105699 1104 8 0.06660533943374247 1104 9 0.24130742246055092 1104 10-11 0.13934055226298625 1104 12-13 0.08808450762318643 1104 14-15 0.05785081306876805 1104 16-17 0.06640598210901061 1104 18-19 0.06159968020884321 1104 20-21 0.06921460525300915 1104 22-23 -0.10284112019262182 1104 24-25 0.12342834982592876 1104 26-27 0.03721969446818463 1104 28-29 0.07856251337157971 1104 30-31 -0.024839673445647747 1104 32-33 -0.04014962773854336 1104 34-35 0.15635824816781252 1104 36-37 0.1166431474530043 1104 38-39 0.23590863093796344 1104 40-41 0.2581047954654778 1104 42-43 0.12574847885698404 1104 44-45 0.0016114927009667213 1104 46-47 0.09937217926707831 1104 48-49 0.042280794845240166 1104 50-51 -0.11958874022639776 1104 52-53 -0.017378997342895275 1104 54-55 -0.05465852092388701 1104 56-57 -0.02540137310763413 1104 58-59 0.08176681296232857 1104 60-61 0.029392182884826923 1104 62-63 0.23001349062600696 1104 64-65 0.17819818784415276 1104 66-67 0.1615939624319438 1104 68-69 0.14991389184192627 1104 70-71 0.20363043753830112 1104 72-73 0.20750915058203034 1104 74-75 0.06061306160430391 1104 76-77 0.25323848547076366 1104 78-79 0.19812172260029826 1104 80-81 0.19344762200984533 1104 82-83 0.17628249297623455 1104 84-85 0.3826675118213707 1104 86-87 0.3350982273879275 1104 88-89 0.19545406272402488 1104 90-91 0.37622885641413006 1104 92-93 0.4231222665472387 1104 94-95 0.48870989558936984 1104 96-97 0.5540693928964302 1104 98-99 0.622878150841256 1104 100-101 1.13490455427754 1105 1 0.060619101085389815 1105 2 0.014621977521699137 1105 3 -0.037352928511854344 1105 4 0.11609939030530825 1105 5 -0.02908609300765619 1105 6 -0.11234329876405269 1105 7 -0.07225317448555302 1105 8 -0.09567468799252765 1105 9 -0.0720671473855461 1105 10-11 -0.05968833433498588 1105 12-13 0.02555190385891848 1105 14-15 0.038229618234872476 1105 16-17 -0.03539463579132729 1105 18-19 -0.059429134101158354 1105 20-21 0.14499910509305636 1105 22-23 0.0801212247860903 1105 24-25 0.021649040693972665 1105 26-27 0.058633253426307874 1105 28-29 -0.0863144864833103 1105 30-31 0.08837651021327986 1105 32-33 0.007984722157900137 1105 34-35 -0.017395741831634837 1105 36-37 0.09918034204306281 1105 38-39 0.105254187412406 1105 40-41 0.08845638474895168 1105 42-43 0.026821307911568226 1105 44-45 0.09488320862865152 1105 46-47 0.1875639117886294 1105 48-49 0.07305173529559283 1105 50-51 -0.07613166498702384 1105 52-53 -0.19146151999897398 1105 54-55 0.0010354517156514476 1105 56-57 -0.09547107705601121 1105 58-59 -0.06376560145059784 1105 60-61 0.1526855258637525 1105 62-63 0.18811155088072695 1105 64-65 0.17791294705237704 1105 66-67 -0.044322106914883364 1105 68-69 0.009289642031944823 1105 70-71 -0.11545892819946602 1105 72-73 0.103012568886097 1105 74-75 0.15855193726737227 1105 76-77 0.11671131840948235 1105 78-79 0.3493441176303378 1105 80-81 0.34255721219637536 1105 82-83 0.31293575939464446 1105 84-85 0.26892643115931847 1105 86-87 0.36127942097026633 1105 88-89 0.4032690400369283 1105 90-91 0.5835712645854798 1105 92-93 0.789534225999077 1105 94-95 0.9620996944615747 1105 96-97 1.1028001185759777 1105 98-99 1.0125981266517599 1105 100-101 1.5272302687821195 1106 1 -0.019219598155032713 1106 2 0.11514122799590609 1106 3 0.0539393288153569 1106 4 0.05226500577298765 1106 5 0.04971331751813324 1106 6 0.021591306511616892 1106 7 -0.36575067079571255 1106 8 -0.18474741400687833 1106 9 -0.2835331409247388 1106 10-11 -0.5517117793829485 1106 12-13 -0.22870751993510652 1106 14-15 -0.17154267794046518 1106 16-17 -0.054790284058213956 1106 18-19 -0.39872862424760314 1106 20-21 -0.20004137202317906 1106 22-23 -0.1121940313757861 1106 24-25 -0.08628759139163833 1106 26-27 -0.3378151588590228 1106 28-29 0.03619919673451477 1106 30-31 -0.1223831665099695 1106 32-33 -0.17714680947906913 1106 34-35 -0.04808331543853939 1106 36-37 -0.438416222621008 1106 38-39 -0.17949192960350757 1106 40-41 -0.299817546322636 1106 42-43 -0.37064149404907454 1106 44-45 -0.13430231988088792 1106 46-47 -0.26861785851337316 1106 48-49 -0.17738153612307173 1106 50-51 -0.151867086951178 1106 52-53 0.04846407548195231 1106 54-55 -0.15899855165036314 1106 56-57 -0.15809173892593975 1106 58-59 -0.07619402022937294 1106 60-61 -0.153087618848339 1106 62-63 -0.24399453647016145 1106 64-65 -0.0541732492015754 1106 66-67 -0.035788066692660436 1106 68-69 -0.30247868492703844 1106 70-71 -0.09068466542203524 1106 72-73 -0.16530369899267683 1106 74-75 0.05729907950474811 1106 76-77 0.19496899898941322 1106 78-79 0.053516240046956653 1106 80-81 -0.25079927396116375 1106 82-83 -0.08580758468419702 1106 84-85 -0.3000975039917684 1106 86-87 -0.1174470930460636 1106 88-89 0.28418396860060113 1106 90-91 0.36453202426220344 1106 92-93 0.6156020571717775 1106 94-95 0.61594509084021 1106 96-97 0.7961092262225442 1106 98-99 0.8481256233501746 1106 100-101 0.9620095563826254 1107 1 0.07554055480902377 1107 2 0.0432079585384173 1107 3 0.1382737498405362 1107 4 0.20827189805544322 1107 5 0.09801365114831384 1107 6 0.12198143358173752 1107 7 0.19181954616248476 1107 8 0.20552892506232467 1107 9 0.12044693251558414 1107 10-11 0.14240615097209997 1107 12-13 0.17447128905640596 1107 14-15 0.2153190635268416 1107 16-17 0.15304954641732138 1107 18-19 0.1490877118587477 1107 20-21 0.20840550428468418 1107 22-23 0.24140143512909873 1107 24-25 0.25005308879034516 1107 26-27 0.1900618029715062 1107 28-29 0.21492961081784756 1107 30-31 0.1784513595667505 1107 32-33 0.2162436178187761 1107 34-35 0.200281485478925 1107 36-37 0.2985171116135703 1107 38-39 0.32019052183197516 1107 40-41 0.21653472614220703 1107 42-43 0.14015586124313728 1107 44-45 0.18083864915059422 1107 46-47 0.06162631168928101 1107 48-49 0.2038579763251107 1107 50-51 0.19845629155150135 1107 52-53 0.06720422866005293 1107 54-55 0.16661095852780505 1107 56-57 0.009864555126007701 1107 58-59 0.10959737838688 1107 60-61 0.2170996545374564 1107 62-63 0.2689715839848077 1107 64-65 0.47234297126359337 1107 66-67 0.543848888693951 1107 68-69 0.26079022618132797 1107 70-71 0.5079991485854691 1107 72-73 0.6286006641105182 1107 74-75 0.5936667689171848 1107 76-77 0.6404856920457043 1107 78-79 0.7247420873453869 1107 80-81 0.8403792691626322 1107 82-83 0.9580360851013481 1107 84-85 0.9340994206172759 1107 86-87 0.9265955550639369 1107 88-89 1.0871516324401718 1107 90-91 1.1079806664498868 1107 92-93 1.15790079334257 1107 94-95 1.183499147322454 1107 96-97 1.3630383245986692 1107 98-99 1.741871902187949 1107 100-101 2.1629530206276435 1108 1 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86-87 0.8828830519566928 2316 88-89 0.6053778189973151 2316 90-91 0.7528984821441469 2316 92-93 0.8826828382794965 2316 94-95 1.0401008025790937 2316 96-97 1.490486703269788 2316 98-99 1.5816625205092372 2316 100-101 1.4329425922271568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7601.0 3 1839.0 4 1326.0 5 1506.0 6 1677.0 7 2027.0 8 2553.0 9 3180.0 10 4137.0 11 5183.0 12 6786.0 13 8400.0 14 9411.0 15 11822.0 16 14668.0 17 18393.0 18 16032.0 19 12041.0 20 12207.0 21 13086.0 22 14240.0 23 15936.0 24 17737.0 25 20476.0 26 23660.0 27 27651.0 28 32993.0 29 39534.0 30 47895.0 31 59427.0 32 75149.0 33 97272.0 34 130791.0 35 181409.0 36 261251.0 37 350563.0 38 257372.0 39 27417.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78386116757371 19.42205975564444 15.189441129543418 22.60463794723843 2 14.764116312912149 20.22781004321967 41.12013887978465 23.88793476408354 3 18.96063150397312 24.859679135505495 29.71820030980924 26.461489050712146 4 12.268644079369631 16.731751949417564 37.960861430892976 33.038742540319824 5 13.436030943659249 37.79127123115866 34.2374044208661 14.535293404315988 6 31.38830348454159 36.6903275052741 17.720112186360932 14.201256823823375 7 28.269190314481563 32.95327444943188 20.64639333826916 18.1311418978174 8 26.054095339662503 35.364389866261355 19.730479397233662 18.85103539684248 9 26.25754389760349 17.0320680181354 20.426692190403138 36.28369589385797 10-11 24.582342814089074 27.172371930027772 27.441895153116835 20.803390102766315 12-13 25.62031402599354 24.34164498261861 28.11943641963926 21.91860457174859 14-15 22.424468559341292 25.69481167945737 26.52780994877891 25.352909812422425 16-17 21.71036168592256 29.265946624035248 26.9819840001049 22.041707689937287 18-19 21.64297684557296 27.65190099034242 29.50747844424322 21.197643719841402 20-21 22.434240032689466 26.68119410518863 29.6403721486169 21.244193713505 22-23 22.01183066692196 26.7080160047504 29.518926089879088 21.761227238448548 24-25 21.84750745152107 26.893538500868395 29.651331905620165 21.607622141990362 26-27 21.746532640312083 27.13602112605847 29.40366357276327 21.71378266086618 28-29 21.530467849357205 27.231340388681573 29.560370214556166 21.677821547405056 30-31 21.694283791879506 26.92696343295386 29.544874038099312 21.83387873706732 32-33 21.511887746627853 27.102346734502987 29.47997801438899 21.90578750448017 34-35 21.993529619393875 27.145219119805308 29.196240163568078 21.66501109723274 36-37 21.948487477220386 26.88338803354558 29.25921454574059 21.908909943493445 38-39 21.69005790290372 27.148483432296434 29.363565173475177 21.797893491324672 40-41 21.98971726858665 27.03008015767146 29.280328977484398 21.699873596257486 42-43 21.85382708630662 27.0561515401897 28.996346701446978 22.093674672056704 44-45 21.867508734431485 27.279700959947416 28.83811640608844 22.01467389953266 46-47 21.93578526790288 27.41798313014377 28.48230031076051 22.163931291192842 48-49 22.08876947900974 27.634744559209796 28.239653223219797 22.036832738560665 50-51 22.07983843580859 27.638989710172922 28.207098103746308 22.074073750272184 52-53 22.214355550139057 27.67667085109255 28.085677076359673 22.02329652240872 54-55 22.00012512768898 27.573962621791175 28.186296004574533 22.239616245945303 56-57 22.062108754369785 27.92247290044884 28.013071042108063 22.002347303073318 58-59 22.203172701083428 27.58066317639872 28.085960979310453 22.1302031432074 60-61 22.150153620632544 27.502558068216793 28.466281424915678 21.881006886234985 62-63 22.0718074596364 27.56369869018173 28.41864043370576 21.945853416476112 64-65 21.9498367935789 27.595935145621418 28.272682661341964 22.18154539945772 66-67 21.885523908353587 27.792430159206972 28.078647696550973 22.243398235888474 68-69 21.87625023041789 27.659459422555692 28.262874386405684 22.20141596062073 70-71 21.879560608576348 27.639418077269273 28.20930298607895 22.271718328075433 72-73 22.033010038577984 27.51195218361875 28.13852352776353 22.316514250039734 74-75 21.92208964085595 27.809050059714867 27.978021431807747 22.29083886762143 76-77 22.004392706064813 27.638512139487126 27.994719575074928 22.36237557937313 78-79 21.974663381096462 27.677696925267846 28.013537408685856 22.334102284949832 80-81 21.906389133627822 27.92826870313735 27.91636100852711 22.248981154707717 82-83 21.975973725563666 27.839110042499872 27.91708018607964 22.26783604585682 84-85 22.011928498920298 27.793255009830265 27.77178928303107 22.423027208218368 86-87 22.0789542683593 27.64276115978762 27.91307055301855 22.365214018834532 88-89 22.117253381489725 27.621316850038944 27.785734652608674 22.47569511586266 90-91 22.017620411379653 27.62900170577382 27.963928824572974 22.38944905827355 92-93 22.09340243373539 27.530552785009117 27.976513656032502 22.399531125222993 94-95 21.977315803560213 27.437008067555535 27.95997902544803 22.62569710343622 96-97 22.025964022921322 27.43093832110608 27.92397069829183 22.619126957680773 98-99 21.862819383115518 27.404574981658225 27.80541714626601 22.927188488960244 100-101 22.074141111046178 27.05403942562868 28.249408364332872 22.622411098992266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2601.0 1 1531.5 2 448.5 3 387.0 4 329.0 5 332.5 6 508.5 7 1020.0 8 1678.5 9 1988.0 10 1872.0 11 1733.5 12 1829.5 13 2029.5 14 2187.0 15 2374.5 16 2523.5 17 2577.0 18 2679.5 19 2941.5 20 3318.0 21 3834.5 22 4657.0 23 5593.5 24 6743.5 25 8538.0 26 10902.5 27 13414.0 28 15848.0 29 18928.0 30 22759.0 31 26606.0 32 30842.5 33 34910.0 34 39333.0 35 42723.0 36 46082.0 37 50024.0 38 52724.0 39 55051.0 40 57124.0 41 60252.0 42 63222.0 43 66436.5 44 69211.0 45 70621.0 46 70088.5 47 70055.5 48 73509.5 49 73068.0 50 68993.0 51 65337.0 52 61264.5 53 59114.5 54 57112.5 55 52791.0 56 47151.0 57 41154.5 58 36545.0 59 32195.0 60 26955.5 61 21565.5 62 16746.0 63 12539.5 64 9288.5 65 7286.0 66 5499.0 67 4159.0 68 2979.5 69 1952.0 70 1431.0 71 1053.5 72 771.0 73 596.5 74 464.0 75 360.5 76 231.0 77 130.5 78 92.5 79 64.5 80 39.0 81 26.0 82 17.5 83 9.5 84 6.5 85 5.5 86 5.5 87 5.5 88 4.0 89 3.5 90 3.0 91 3.5 92 3.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24696795194290355 2 0.21818863642185898 3 0.2096856568360958 4 0.2167169668781692 5 0.21633542292239777 6 0.2442971442525036 7 0.2450602321640465 8 0.2338319386084874 9 0.22881736090406296 10-11 0.23884651631291184 12-13 0.2392008071289853 14-15 0.2264735908900385 16-17 0.23922806026868323 18-19 0.23020727102865887 20-21 0.22303969528809567 22-23 0.2218133040016875 24-25 0.21718027025303452 26-27 0.22432059285389971 28-29 0.23770188444559753 30-31 0.23740209990892 32-33 0.2378108970043894 34-35 0.24337053750277302 36-37 0.2216770383031977 38-39 0.22273991075141814 40-41 0.21851567409823447 42-43 0.2242115802951079 44-45 0.2395278448053608 46-47 0.24328877808367913 48-49 0.24745850845746686 50-51 0.24759477415595665 52-53 0.26435545507020136 54-55 0.24639563600924644 56-57 0.22017811561981 58-59 0.20442580087438972 60-61 0.20055585503727957 62-63 0.20845926554968766 64-65 0.2009919052724469 66-67 0.2017277400442918 68-69 0.20377172552163872 70-71 0.20467107913167137 72-73 0.20788694961603052 74-75 0.21080303556371213 76-77 0.22437509913329565 78-79 0.21911524317158956 80-81 0.21268350220287127 82-83 0.20832299985119787 84-85 0.20851377182908357 86-87 0.2164444354811896 88-89 0.21524529733447942 90-91 0.22366651750114871 92-93 0.2117568954531407 94-95 0.21044874474763867 96-97 0.22025987503890387 98-99 0.22511093390514053 100-101 0.20221829655885504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1834651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.94411373703292 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86243391767074 45.89532160685393 2 12.872265109717821 15.174885173608955 3 4.036364693861154 7.137598187972877 4 1.7771282771519157 4.190050051749595 5 0.9568162531303119 2.819934302497739 6 0.5635758078344763 1.9931685909861343 7 0.3618172133700027 1.492889648382842 8 0.2582158396080084 1.217624305484632 9 0.19188185404698824 1.0179275246116584 >10 1.0068185826109832 10.799865169328235 >50 0.07549694180510863 3.042261329985328 >100 0.03552089427653334 3.721555914060942 >500 0.0011097432773646426 0.38190192893581953 >1k 4.623930322352674E-4 0.7373116601178835 >5k 9.247860644705348E-5 0.37770460542339307 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6929 0.3776740099343145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4874 0.2656636057757034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3617 0.19714921257503473 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2684 0.14629485389864338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 5.450627939591781E-5 0.0 0.0 0.0 0.0 16-17 5.450627939591781E-5 0.0 0.0 0.0 0.0 18-19 8.175941909387671E-5 0.0 0.0 0.0 0.0 20-21 1.0901255879183562E-4 0.0 0.0 2.7253139697958904E-5 0.0 22-23 1.0901255879183562E-4 0.0 0.0 5.450627939591781E-5 5.450627939591781E-5 24-25 1.0901255879183562E-4 0.0 0.0 1.362656984897945E-4 5.450627939591781E-5 26-27 1.0901255879183562E-4 0.0 0.0 3.8154395577142465E-4 5.450627939591781E-5 28-29 1.0901255879183562E-4 0.0 0.0 0.0010628724482203973 5.450627939591781E-5 30-31 1.0901255879183562E-4 0.0 0.0 0.0023982762934203834 8.175941909387671E-5 32-33 1.0901255879183562E-4 0.0 0.0 0.004605780608955055 1.0901255879183562E-4 34-35 1.0901255879183562E-4 0.0 0.0 0.007467360277240739 1.6351883818775342E-4 36-37 1.0901255879183562E-4 0.0 0.0 0.011800609489216206 1.6351883818775342E-4 38-39 1.0901255879183562E-4 0.0 0.0 0.017360249987599823 1.6351883818775342E-4 40-41 1.0901255879183562E-4 0.0 0.0 0.02335594072115078 1.6351883818775342E-4 42-43 1.6351883818775342E-4 0.0 0.0 0.03000570680745275 1.6351883818775342E-4 44-45 2.1802511758367123E-4 0.0 0.0 0.036600966614358806 1.6351883818775342E-4 46-47 2.72531396979589E-4 0.0 0.0 0.04553999643528933 1.6351883818775342E-4 48-49 2.72531396979589E-4 0.0 0.0 0.054206494859240256 1.6351883818775342E-4 50-51 2.72531396979589E-4 0.0 0.0 0.06322728409926465 1.6351883818775342E-4 52-53 2.72531396979589E-4 0.0 0.0 0.08241349444662772 1.6351883818775342E-4 54-55 3.2703767637550684E-4 0.0 0.0 0.10751363610844789 1.6351883818775342E-4 56-57 3.2703767637550684E-4 0.0 0.0 0.1464856258765291 1.6351883818775342E-4 58-59 3.2703767637550684E-4 0.0 0.0 0.19581380872983473 1.6351883818775342E-4 60-61 3.2703767637550684E-4 0.0 0.0 0.2530454020955484 1.6351883818775342E-4 62-63 3.5429081607346575E-4 0.0 0.0 0.30357272309556427 1.6351883818775342E-4 64-65 3.8154395577142465E-4 0.0 0.0 0.3638566681074493 1.6351883818775342E-4 66-67 3.8154395577142465E-4 0.0 0.0 0.44932251420024844 1.6351883818775342E-4 68-69 3.8154395577142465E-4 0.0 0.0 0.5599157550945657 1.6351883818775342E-4 70-71 3.8154395577142465E-4 2.7253139697958904E-5 0.0 0.6791482412731359 2.1802511758367123E-4 72-73 3.8154395577142465E-4 5.450627939591781E-5 0.0 0.8084098828605549 2.1802511758367123E-4 74-75 3.8154395577142465E-4 5.450627939591781E-5 0.0 0.9451388847252147 2.1802511758367123E-4 76-77 3.8154395577142465E-4 5.450627939591781E-5 0.0 1.0885721589555724 2.1802511758367123E-4 78-79 3.8154395577142465E-4 5.450627939591781E-5 0.0 1.2376468331034076 2.1802511758367123E-4 80-81 4.3605023516734247E-4 5.450627939591781E-5 0.0 1.4044905543343122 2.452782572816301E-4 82-83 4.3605023516734247E-4 5.450627939591781E-5 0.0 1.5947992288451591 3.2703767637550684E-4 84-85 4.905565145632602E-4 5.450627939591781E-5 0.0 1.8332642012022995 3.2703767637550684E-4 86-87 4.905565145632602E-4 5.450627939591781E-5 0.0 2.113617249275203 3.2703767637550684E-4 88-89 4.905565145632602E-4 5.450627939591781E-5 0.0 2.4316341364106853 3.2703767637550684E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8365 0.0 36.867462 1 GGTATCA 5145 0.0 36.758877 1 ATCAACG 10595 0.0 28.922 3 TATCAAC 10665 0.0 28.820475 2 TCAACGC 10695 0.0 28.741203 4 CAACGCA 10845 0.0 28.210709 5 AACGCAG 11090 0.0 27.585218 6 GTACATG 7890 0.0 25.957615 1 ACGCAGA 12310 0.0 24.582565 7 TACATGG 8260 0.0 24.386166 2 CGCAGAG 12470 0.0 24.152864 8 ACATGGG 8045 0.0 23.503178 3 GAGTACT 6495 0.0 22.160503 12-13 GCAGAGT 13210 0.0 22.150732 9 AGTACTT 6890 0.0 21.33819 12-13 AGAGTAC 11170 0.0 20.880102 10-11 CATGGGG 3740 0.0 20.706059 4 GTACTTT 6965 0.0 20.425285 14-15 GTGTAGC 1015 0.0 20.131073 1 CGCGCGT 120 0.0047612446 19.794046 7 >>END_MODULE