FastQCFastQC Report
Thu 26 May 2016
SRR937280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834651
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89080.48554193685883584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71400.3891748348868531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.2776549872428053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55700.037.8848531
GTATCAA93950.037.4851531
ATCAACG119150.029.3002093
TCAACGC120250.029.0321834
TATCAAC120650.028.9752982
CAACGCA121850.028.6509635
AACGCAG125000.027.9669576
ACGCAGA141200.024.6607277
CGCAGAG141950.024.3631028
GGACCGT1601.8611681E-623.7491156
GTACATG78200.023.2163811
GCAGAGT150250.022.7326989
GAGTACT80650.022.35342212-13
AGTACTT84950.022.17258812-13
TACATGG79100.021.7975442
AGAGTAC129100.021.28696310-11
GTACTTT84450.021.06345414-15
ACATGGG80600.020.331133
TACTTTT93250.019.8397414-15
CAGAGTA147100.018.45610810-11
Produced by FastQC (version 0.11.5)