##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937280_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1834651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15694483582981 33.0 31.0 34.0 31.0 34.0 2 32.362406801075515 34.0 31.0 34.0 31.0 34.0 3 32.41834768574514 34.0 31.0 34.0 31.0 34.0 4 36.07638619007103 37.0 35.0 37.0 35.0 37.0 5 36.00075382184404 37.0 35.0 37.0 35.0 37.0 6 36.04707707351425 37.0 35.0 37.0 35.0 37.0 7 35.98866269388565 37.0 35.0 37.0 35.0 37.0 8 35.96469628283526 37.0 35.0 37.0 35.0 37.0 9 37.671220847997795 39.0 37.0 39.0 35.0 39.0 10-11 37.58586346940099 39.0 37.0 39.0 35.0 39.0 12-13 37.4886490673158 39.0 37.0 39.0 35.0 39.0 14-15 38.67603293487426 40.0 38.0 41.0 34.0 41.0 16-17 38.65053707762403 40.0 38.0 41.0 34.0 41.0 18-19 38.63905696505766 40.0 38.0 41.0 34.0 41.0 20-21 38.54859534592683 40.0 38.0 41.0 34.0 41.0 22-23 38.40008126886258 40.0 38.0 41.0 34.0 41.0 24-25 38.339727828344465 40.0 38.0 41.0 34.0 41.0 26-27 38.30565568056268 40.0 38.0 41.0 34.0 41.0 28-29 38.18703148446217 40.0 38.0 41.0 34.0 41.0 30-31 38.252500066770196 40.0 38.0 41.0 34.0 41.0 32-33 38.19428545265558 40.0 38.0 41.0 34.0 41.0 34-35 38.071340816318745 40.0 37.5 41.0 33.5 41.0 36-37 37.90308320220031 40.0 37.0 41.0 33.0 41.0 38-39 37.78626861457575 40.0 37.0 41.0 33.0 41.0 40-41 37.60497800399095 40.0 37.0 41.0 33.0 41.0 42-43 37.397636389700274 40.0 37.0 41.0 32.5 41.0 44-45 37.12014192345029 40.0 36.5 41.0 31.5 41.0 46-47 36.779819431597616 39.5 36.0 41.0 31.0 41.0 48-49 36.462785020148246 39.0 36.0 41.0 30.0 41.0 50-51 36.22102078269927 39.0 35.5 41.0 30.0 41.0 52-53 35.97401304117241 39.0 35.0 40.0 29.0 41.0 54-55 35.748204426891 39.0 35.0 40.0 28.5 41.0 56-57 35.48418445797048 38.0 35.0 40.0 28.0 41.0 58-59 35.217002034719414 38.0 34.5 40.0 28.0 41.0 60-61 34.8861391076559 38.0 34.0 40.0 27.0 41.0 62-63 34.51677103710733 37.5 34.0 40.0 26.0 41.0 64-65 34.1100086610478 37.0 33.5 39.5 26.0 41.0 66-67 33.69051334558998 36.0 33.0 39.0 25.5 40.5 68-69 33.262590541743364 36.0 32.5 39.0 24.5 40.0 70-71 32.848943477533325 35.0 32.0 38.5 23.5 40.0 72-73 32.3635631517929 35.0 31.5 37.5 22.0 39.5 74-75 31.886988860551682 35.0 31.0 37.0 21.5 39.0 76-77 30.628311324606152 33.5 29.5 35.5 20.5 38.0 78-79 31.24508176214441 34.0 31.0 36.0 20.5 38.5 80-81 31.05305041667325 34.0 31.0 36.0 20.0 37.5 82-83 30.703329952127135 34.0 31.0 35.0 18.5 37.0 84-85 30.3341485655855 34.0 30.5 35.0 17.5 36.5 86-87 29.903556044174067 34.0 30.0 35.0 13.0 36.0 88-89 29.42045953154033 34.0 29.5 35.0 7.0 36.0 90-91 28.887876222780246 34.0 29.0 35.0 2.0 35.0 92-93 28.505047008940664 33.5 29.0 35.0 2.0 35.0 94-95 28.032598297986922 33.0 28.5 35.0 2.0 35.0 96-97 27.51054696506311 33.0 27.0 35.0 2.0 35.0 98-99 26.6952030658692 33.0 25.5 34.5 2.0 35.0 100-101 24.092530677496704 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05838381524058178 1101 2 -0.03002601514074854 1101 3 -0.07729934934616978 1101 4 0.0321343471809854 1101 5 0.0023235562645140817 1101 6 -0.010546384679692267 1101 7 -0.07763595546369828 1101 8 -0.056049561051942476 1101 9 -0.09533599428143447 1101 10-11 -0.11952047544748723 1101 12-13 -0.07908198852999959 1101 14-15 -0.10275265252114707 1101 16-17 -0.15039877586881545 1101 18-19 -0.04398791262533308 1101 20-21 -0.09055206825772899 1101 22-23 -0.006461821753788399 1101 24-25 -0.0625419102034428 1101 26-27 -0.06969190923292956 1101 28-29 -0.23509734309192964 1101 30-31 -0.003746865669398858 1101 32-33 0.09884765830623365 1101 34-35 0.005823468569047918 1101 36-37 -0.06379164724567232 1101 38-39 -0.06694825184020914 1101 40-41 -0.06036784206589374 1101 42-43 -0.025502367177097085 1101 44-45 0.045776066132752646 1101 46-47 0.06855316536371703 1101 48-49 0.09622614744319691 1101 50-51 0.14416472913320177 1101 52-53 0.07783681000674392 1101 54-55 0.12786947767023094 1101 56-57 0.1324731062747304 1101 58-59 0.21126885233399406 1101 60-61 0.21488986150323086 1101 62-63 0.2740794479671038 1101 64-65 0.019826539020428413 1101 66-67 0.0881517216595995 1101 68-69 0.06722429089917625 1101 70-71 0.18656772782880182 1101 72-73 0.30638416999970985 1101 74-75 0.14807995297398335 1101 76-77 0.29378582765060557 1101 78-79 0.06612673273948744 1101 80-81 -0.07656204981036296 1101 82-83 -0.14025127247847635 1101 84-85 -0.08548735115308759 1101 86-87 -0.04158769523078121 1101 88-89 -0.014119493071561351 1101 90-91 -0.21723806222940212 1101 92-93 0.02574903504819659 1101 94-95 -0.0971616729693956 1101 96-97 -0.06610445231575213 1101 98-99 0.058346938514578284 1101 100-101 0.9869649597266346 1102 1 -0.05232520822620046 1102 2 -0.06381044214337805 1102 3 -0.07181603727336494 1102 4 -0.06386478009967078 1102 5 -0.11581464934013042 1102 6 -0.05336546368292261 1102 7 -0.09999655836044496 1102 8 -0.01783284158129561 1102 9 -0.0818129611289038 1102 10-11 -0.04049332080584378 1102 12-13 -0.050134438653522295 1102 14-15 -0.07303568425344764 1102 16-17 -0.1403457797811356 1102 18-19 -0.07307053846081857 1102 20-21 0.046027865063628326 1102 22-23 -0.027377369176768696 1102 24-25 -0.019166060961588016 1102 26-27 -0.02843083493394971 1102 28-29 -0.16199230442418866 1102 30-31 -0.10717452747624634 1102 32-33 -0.06879074052135792 1102 34-35 -0.10683193953787651 1102 36-37 -0.13363350803150098 1102 38-39 -0.10741572271912503 1102 40-41 -0.08548476638888047 1102 42-43 -0.04125466822821977 1102 44-45 -0.011200131167690586 1102 46-47 -0.01898647621415961 1102 48-49 -0.03354788720108104 1102 50-51 0.056690953165805524 1102 52-53 -0.001504750009942768 1102 54-55 -0.08353391780490682 1102 56-57 -0.0030217168426602825 1102 58-59 0.10459859564850404 1102 60-61 0.09992619822264004 1102 62-63 -0.056672562349099564 1102 64-65 0.03272989513056501 1102 66-67 3.4694527077760995E-4 1102 68-69 0.12025871442843084 1102 70-71 0.05550532582319079 1102 72-73 -0.015565133034073142 1102 74-75 0.14646573094203674 1102 76-77 0.2510355730645806 1102 78-79 0.11855022700274631 1102 80-81 0.13639369004311774 1102 82-83 0.0668685427273168 1102 84-85 0.1219848193400459 1102 86-87 0.21100550589449085 1102 88-89 0.3791859365703303 1102 90-91 0.330310476351638 1102 92-93 0.48593829061757177 1102 94-95 0.37374849714398195 1102 96-97 0.5436932630737985 1102 98-99 0.7926472527655797 1102 100-101 1.3401250424908895 1103 1 0.015706807088967878 1103 2 0.04302251300489246 1103 3 -0.01503321855759765 1103 4 -0.03400104319364772 1103 5 0.015121382501533276 1103 6 -0.07073956405955073 1103 7 -0.11247507699034287 1103 8 -0.12875241893169687 1103 9 -0.03817679386063588 1103 10-11 -0.04798572622163988 1103 12-13 -0.029453557311164502 1103 14-15 -0.17715296009276926 1103 16-17 -0.27490227618157803 1103 18-19 -0.16355276941029473 1103 20-21 -0.2302882030541511 1103 22-23 -0.1588002061894187 1103 24-25 -0.031172333574602362 1103 26-27 -0.14934937461373465 1103 28-29 -0.16838271422996343 1103 30-31 -0.26429877159144155 1103 32-33 -0.2647969263088754 1103 34-35 -0.24251346148302133 1103 36-37 -0.2477105688400556 1103 38-39 -0.1693477171749933 1103 40-41 -0.2429242823684845 1103 42-43 -0.11956226980082363 1103 44-45 -0.3267449135374534 1103 46-47 -0.135621712755416 1103 48-49 -0.28527169104745553 1103 50-51 -0.1796493837383153 1103 52-53 -0.26661371071929096 1103 54-55 -0.2655476976472073 1103 56-57 -0.4111412776750427 1103 58-59 -0.2602090699843771 1103 60-61 -0.14719505994239768 1103 62-63 -0.16723927874630817 1103 64-65 -0.3385928596096264 1103 66-67 -0.03026883812289327 1103 68-69 -0.3183821276518728 1103 70-71 -0.3115931565305843 1103 72-73 -0.16426756827760158 1103 74-75 -0.08138423671016781 1103 76-77 0.19078291085707733 1103 78-79 -0.15041214473110642 1103 80-81 -0.33680052483729384 1103 82-83 -0.31160566851065497 1103 84-85 -0.17764098392819605 1103 86-87 -0.11420761365046062 1103 88-89 -0.1098753738805982 1103 90-91 0.007234375917828828 1103 92-93 0.04220756826116556 1103 94-95 -0.08746591631269851 1103 96-97 0.15796118115711621 1103 98-99 0.37141651187934244 1103 100-101 1.0520356482258215 1104 1 0.11611646341297899 1104 2 0.056983098067327376 1104 3 0.08668998024862873 1104 4 0.044769273128608233 1104 5 -0.03680740248791636 1104 6 0.05134733332711505 1104 7 0.035448973615153534 1104 8 -2.7648556392989576E-4 1104 9 -0.04097118493677954 1104 10-11 0.07749087961924062 1104 12-13 -0.054083291018308444 1104 14-15 -0.013354591571008712 1104 16-17 0.0039263536300993 1104 18-19 0.0944773825079821 1104 20-21 0.057400341187758386 1104 22-23 0.04552982758584534 1104 24-25 0.08018423202617697 1104 26-27 -0.029589873088482932 1104 28-29 0.04380908246442061 1104 30-31 0.07262920664685879 1104 32-33 0.11270494979364543 1104 34-35 0.11080428909983908 1104 36-37 0.039734596446471926 1104 38-39 -0.015526802936435047 1104 40-41 0.0716528988105054 1104 42-43 0.1341758576918508 1104 44-45 0.176453665281052 1104 46-47 -0.022948085288184927 1104 48-49 0.15158216354458887 1104 50-51 0.0771240772891062 1104 52-53 0.16469278216560213 1104 54-55 0.10218111917913575 1104 56-57 0.1870376304928527 1104 58-59 -0.1033493028268353 1104 60-61 0.06965504785904386 1104 62-63 0.07213245161038628 1104 64-65 0.14890628761650504 1104 66-67 0.06343720341450876 1104 68-69 0.03602798565064802 1104 70-71 0.15165472615372266 1104 72-73 0.29552075971168534 1104 74-75 0.39462980617433985 1104 76-77 0.4086517450312712 1104 78-79 0.19885371171916688 1104 80-81 0.25154251658207016 1104 82-83 0.3138923243574503 1104 84-85 0.25444762442065283 1104 86-87 0.39861296164433213 1104 88-89 0.366078027306461 1104 90-91 0.5235342686036795 1104 92-93 0.5112965458746324 1104 94-95 0.7684358744582838 1104 96-97 0.7335727147748159 1104 98-99 0.9477441048262811 1104 100-101 1.382054263061164 1105 1 0.019270538755385758 1105 2 -0.0034132342350901013 1105 3 0.004928505746590872 1105 4 -0.029972715448813858 1105 5 0.03229882145267027 1105 6 -0.05864745007060179 1105 7 0.01967562985940674 1105 8 -0.07643105667236938 1105 9 -0.07292112065982082 1105 10-11 0.03001211151421046 1105 12-13 -0.04420667687910651 1105 14-15 0.0740164836370738 1105 16-17 -0.08235311922873478 1105 18-19 0.050311858153143874 1105 20-21 -0.060025807745965665 1105 22-23 -0.17435265590525972 1105 24-25 -0.08435020914732405 1105 26-27 -0.043590138504029596 1105 28-29 -0.08012810899676026 1105 30-31 -0.05390010208051166 1105 32-33 -0.08395057157797936 1105 34-35 -0.10548051904302014 1105 36-37 0.06374364431135149 1105 38-39 -0.07570006749908487 1105 40-41 -0.08478663796384467 1105 42-43 -0.09285828003400098 1105 44-45 -0.002403370145991346 1105 46-47 -0.1904598199460068 1105 48-49 -0.0942454986893182 1105 50-51 -0.2108521939137873 1105 52-53 -0.24823446538349003 1105 54-55 -0.041421503040140806 1105 56-57 -0.04019422023571906 1105 58-59 -0.09537537289114795 1105 60-61 0.014287045429306033 1105 62-63 0.06175831818944033 1105 64-65 0.21666088828748542 1105 66-67 0.18003918852858192 1105 68-69 0.19672711824890854 1105 70-71 0.2549159387467199 1105 72-73 0.43039703668682705 1105 74-75 0.2911753311441707 1105 76-77 0.44882996725421265 1105 78-79 0.3881748359374164 1105 80-81 0.2732436090475616 1105 82-83 0.14276093125490874 1105 84-85 0.2760347554350524 1105 86-87 0.31281185714882653 1105 88-89 0.6719755121704942 1105 90-91 0.7619002205364929 1105 92-93 0.9050320107321603 1105 94-95 1.004212136706105 1105 96-97 0.7799186116500856 1105 98-99 1.0705468066702188 1105 100-101 1.622622300879641 1106 1 -0.11394382312303009 1106 2 0.04324672141366648 1106 3 -0.053628126485314453 1106 4 -0.03991348105628845 1106 5 -0.10384687478354948 1106 6 -0.03409080771027817 1106 7 -0.0454757309785947 1106 8 -0.07518919950226177 1106 9 -0.032997410190915843 1106 10-11 -0.23259847820195034 1106 12-13 -0.12143491582209975 1106 14-15 -0.07968894356573486 1106 16-17 0.0045353842799187305 1106 18-19 -0.001466467809883909 1106 20-21 0.014700710771883507 1106 22-23 -0.06191506527697754 1106 24-25 -0.125743666298753 1106 26-27 -0.06344564529936036 1106 28-29 -0.0294384478093761 1106 30-31 -0.0465644512140031 1106 32-33 -0.02644813934978174 1106 34-35 -0.11514247167913538 1106 36-37 0.013401866311752997 1106 38-39 -0.013081515717246361 1106 40-41 0.06871411069540301 1106 42-43 0.05327586980025956 1106 44-45 0.05805149732078263 1106 46-47 -0.15676930965918956 1106 48-49 -0.12363775956147549 1106 50-51 0.010775561790900667 1106 52-53 0.19446918765726195 1106 54-55 0.04352782952493328 1106 56-57 -0.08304982775258196 1106 58-59 0.057475890657734396 1106 60-61 0.025768738023792537 1106 62-63 0.18483089340282532 1106 64-65 0.14382908819104046 1106 66-67 0.195832635101425 1106 68-69 0.28588579518429924 1106 70-71 0.26716809483471593 1106 72-73 0.20556307248404693 1106 74-75 0.26265622471864347 1106 76-77 0.3910817801012172 1106 78-79 0.3752520938582684 1106 80-81 0.2634076444812834 1106 82-83 0.12175134695351275 1106 84-85 0.1846670976271909 1106 86-87 0.3459541665530139 1106 88-89 0.49098763082834296 1106 90-91 0.4164734566294648 1106 92-93 0.6199031237389114 1106 94-95 0.6993443604519669 1106 96-97 0.6271201003031557 1106 98-99 0.6396434695237225 1106 100-101 1.2133036173720342 1107 1 0.012648355522046018 1107 2 -0.08676427942617693 1107 3 0.03415074593142009 1107 4 -6.205427167600419E-4 1107 5 -0.00952523456001586 1107 6 -0.06231756583093073 1107 7 -0.07711427030589135 1107 8 -0.051421639981974465 1107 9 0.01405244394797478 1107 10-11 0.06310415326878172 1107 12-13 -0.02934991588687552 1107 14-15 0.04026766704904361 1107 16-17 0.15136913641122618 1107 18-19 0.09735140661155839 1107 20-21 0.037825709715740174 1107 22-23 0.04698142036870223 1107 24-25 0.008025814003097764 1107 26-27 -0.10374220608646567 1107 28-29 -0.04782838223806607 1107 30-31 0.026122344792803176 1107 32-33 -0.01894244600678263 1107 34-35 0.056187300604037205 1107 36-37 0.13070458768182647 1107 38-39 0.017931924684177147 1107 40-41 -0.06384178489293646 1107 42-43 -0.01378940128623185 1107 44-45 0.1544412165768776 1107 46-47 0.018147705254754953 1107 48-49 0.2015090168770115 1107 50-51 0.24623423147224344 1107 52-53 0.27919607336062313 1107 54-55 0.36013571802271827 1107 56-57 0.34434265776692285 1107 58-59 0.1860146208803073 1107 60-61 0.21354175233840778 1107 62-63 0.1908277029902905 1107 64-65 0.2384425069625422 1107 66-67 0.2183740769493454 1107 68-69 0.27072753348598155 1107 70-71 0.26104342090287247 1107 72-73 0.4372474908899804 1107 74-75 0.47218025955407583 1107 76-77 0.2869718990037917 1107 78-79 0.34069830380701305 1107 80-81 0.45254477983554864 1107 82-83 0.46730713157588255 1107 84-85 0.47160088248501 1107 86-87 0.4854740218578897 1107 88-89 0.5938626634281192 1107 90-91 0.761804070451543 1107 92-93 0.8895138246889935 1107 94-95 0.5144553348740288 1107 96-97 0.7613866743667508 1107 98-99 1.0250519510416929 1107 100-101 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2316 14-15 0.1459037434921271 2316 16-17 0.1113668521660216 2316 18-19 -0.08784545277523392 2316 20-21 -0.05679410902268245 2316 22-23 0.07988182951732625 2316 24-25 0.10292743096503898 2316 26-27 0.2694422482066088 2316 28-29 0.3017768389791371 2316 30-31 0.17687730276194458 2316 32-33 0.08359571619104855 2316 34-35 0.006153186223073703 2316 36-37 0.06794028520894102 2316 38-39 0.10622098770321031 2316 40-41 -0.06906307674557155 2316 42-43 0.04762693524727979 2316 44-45 -0.02646447171621702 2316 46-47 2.845272546636579E-5 2316 48-49 -0.3410374760974406 2316 50-51 -0.23902865454466848 2316 52-53 -0.0037633081314680794 2316 54-55 0.0773584789585442 2316 56-57 0.008071344676778835 2316 58-59 -0.07799373676979116 2316 60-61 -0.004469857001915045 2316 62-63 0.19387840203650342 2316 64-65 -0.01115364445752931 2316 66-67 0.11774374471110605 2316 68-69 0.4119409155797342 2316 70-71 0.2781381222751449 2316 72-73 0.4771560912224686 2316 74-75 0.5367579615884672 2316 76-77 0.1830301577072504 2316 78-79 0.19636647912059502 2316 80-81 0.34719460728545926 2316 82-83 0.26318892114016634 2316 84-85 0.2870654091274467 2316 86-87 0.02205926936630931 2316 88-89 0.08127038850651758 2316 90-91 0.29885008604860985 2316 92-93 0.45555463802958585 2316 94-95 0.4422285839739537 2316 96-97 0.47719895121428024 2316 98-99 0.6689127434657571 2316 100-101 0.1555854414308513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 8.0 5 48.0 6 68.0 7 158.0 8 404.0 9 803.0 10 1364.0 11 2361.0 12 3498.0 13 4968.0 14 6612.0 15 9196.0 16 12559.0 17 17385.0 18 15550.0 19 9774.0 20 8508.0 21 8744.0 22 9205.0 23 10155.0 24 11627.0 25 14113.0 26 17548.0 27 21629.0 28 27267.0 29 34183.0 30 43253.0 31 54057.0 32 70521.0 33 92760.0 34 126844.0 35 181726.0 36 276403.0 37 410748.0 38 306733.0 39 23862.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.260424837421304 20.045154385670163 15.867855510329651 21.826565266578882 2 15.57173544178157 20.867129497653778 40.062714925072946 23.49842013549171 3 19.26148351920883 25.018218723888086 30.207271028658855 25.513026728244228 4 13.022967310949058 17.385649913798318 37.51716266472479 32.07422011052783 5 13.53304197195474 37.757534860346034 34.29472050129564 14.414702666403576 6 31.456500446133894 37.15545899465348 17.20392597829233 14.184114580920296 7 28.246680158787697 33.09332401639331 20.615364993124032 18.044630831694967 8 26.300042896441884 35.55608123833906 19.376382756175424 18.767493109043627 9 26.144700000163517 16.981104308121818 20.287509722557587 36.58668596915708 10-11 24.7832009191954 27.29665309094715 27.188403443058284 20.73174254679917 12-13 25.802139219616826 24.390510466477426 28.109710977775173 21.69763933613057 14-15 22.521449583599278 25.72358448555066 26.644331810246197 25.110634120603866 16-17 21.692872377362235 29.302057993591152 27.103083910781944 21.901985718264672 18-19 21.603182294616254 27.406193330502642 29.991671440508306 20.998952934372806 20-21 22.28936184593146 26.389896498026054 30.306635975997615 21.01410568004487 22-23 21.998018137638052 26.41777287944543 30.12071046168236 21.46349852123416 24-25 21.87295514558614 26.599697869140766 30.271026588115184 21.25632039715791 26-27 21.74279540259707 26.859154457443935 30.076235477772396 21.321814662186597 28-29 21.53372649730582 26.928052846296218 30.21745570636925 21.32076495002871 30-31 21.66114470678107 26.652411407223582 30.18595700692968 21.500486879065672 32-33 21.52679174404287 26.781278837228445 30.094279511471118 21.597649907257566 34-35 22.061961648291692 26.732495717169098 29.874019636432216 21.331522998106998 36-37 22.07624512270334 26.57108432487204 29.888150236952747 21.464520315471873 38-39 21.809461749842406 26.849116679325828 29.915141367009706 21.42628020382206 40-41 21.980462186877862 26.67893968732413 29.950385564529427 21.39021256126858 42-43 21.89074321250596 26.775716486096567 29.621818104508694 21.711722196888775 44-45 21.93361395036376 26.944331267995626 29.370383138995365 21.75167164264525 46-47 22.020782154208078 27.16347686835262 28.982160639816513 21.833580337622795 48-49 22.187462356600793 27.314786299955685 28.751653584251173 21.74609775919235 50-51 22.17827804852258 27.374252650776633 28.586935607916708 21.860533692784077 52-53 22.343759112768584 27.514170269985954 28.388996054290434 21.753074562955025 54-55 22.122790656097536 27.352068595062494 28.492040175488416 22.033100573351554 56-57 22.17301819256087 27.653188535585244 28.355992502116205 21.817800769737676 58-59 22.322065613569013 27.37286274065204 28.412978817224637 21.892092828554315 60-61 22.25663082515421 27.320127915336485 28.82883447587579 21.594406783633506 62-63 22.239216068887217 27.324270392570575 28.72579035467781 21.710723183864395 64-65 22.08200474204889 27.36140408252255 28.599841931703594 21.95674924372496 66-67 22.039232529783597 27.609338233811226 28.35225882197759 21.999170414427592 68-69 22.027486426573773 27.481820793164474 28.51907529006879 21.97161749019296 70-71 22.066131378665478 27.401151499658518 28.464269226136196 22.068447895539805 72-73 22.17225510464933 27.332446334479965 28.367193542532064 22.128105018338637 74-75 22.130585054051153 27.64920957718934 28.07487636613176 22.145329002627747 76-77 22.165114782048466 27.44094108361754 28.31573961478232 22.078204519551676 78-79 22.10766516356517 27.42557031282789 28.3039935115725 22.16277101203444 80-81 22.081759463657917 27.68836017769057 28.18256888234813 22.04731147630338 82-83 22.113363682545497 27.651468077730613 28.16838035062933 22.06678788909456 84-85 22.042714043744216 27.541714530215017 28.10648396077165 22.30908746526912 86-87 22.102541573301952 27.38681634817739 28.2252319378454 22.285410140675257 88-89 22.16159912702743 27.369892148424956 28.118617655346984 22.349891069200627 90-91 22.078037825123538 27.382440399128917 28.243331271003736 22.296190504743812 92-93 22.115674189227306 27.23822865295699 28.300153848879244 22.34594330893645 94-95 21.95300359577925 27.22370630708511 28.22708515134486 22.59620494579078 96-97 21.996227075340215 27.18064634636233 28.17862906896189 22.64449750933556 98-99 21.859194865066833 27.15437666823463 28.022335607519476 22.964092859179058 100-101 21.735832435468918 27.102495004621286 28.390922488566712 22.770750071343084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 269.0 1 207.5 2 210.5 3 290.5 4 255.5 5 241.5 6 441.5 7 1176.5 8 2114.5 9 2533.5 10 2279.0 11 1945.5 12 2072.5 13 2258.0 14 2409.0 15 2585.5 16 2787.0 17 3010.5 18 3130.0 19 3297.5 20 3594.0 21 4059.0 22 4780.0 23 5677.5 24 6907.0 25 8698.0 26 10850.5 27 13364.5 28 15846.0 29 19008.0 30 22770.0 31 26611.5 32 30781.0 33 34611.0 34 38898.5 35 42372.5 36 45974.0 37 49895.5 38 52839.0 39 55140.5 40 57008.0 41 60195.5 42 63264.5 43 66553.0 44 69888.5 45 71127.5 46 70091.0 47 70425.0 48 74217.5 49 73389.5 50 69238.5 51 66107.5 52 62034.0 53 59308.5 54 56782.0 55 52540.0 56 47002.5 57 40995.5 58 36001.0 59 31496.5 60 26507.0 61 21086.5 62 16353.0 63 12400.5 64 9185.0 65 7079.5 66 5213.5 67 3896.5 68 2763.5 69 1767.0 70 1265.0 71 903.0 72 684.0 73 556.0 74 423.0 75 334.0 76 226.0 77 116.0 78 62.0 79 37.0 80 24.0 81 13.5 82 10.5 83 9.0 84 4.5 85 2.0 86 1.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07549119696334616 2 0.0 3 0.0 4 0.0 5 0.0024527825728163015 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.6351883818775342E-4 12-13 0.005641399917477493 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.085816321469315E-4 24-25 8.44847330636726E-4 26-27 3.5429081607346575E-4 28-29 0.0025890482713060957 30-31 3.5429081607346575E-4 32-33 0.0 34-35 0.0 36-37 1.3626569848979454E-4 38-39 6.268222130530548E-4 40-41 1.9077197788571233E-4 42-43 0.012509191121363138 44-45 1.9077197788571233E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.450627939591781E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.450627939591781E-5 82-83 3.8154395577142465E-4 84-85 4.633033748653014E-4 86-87 0.0 88-89 0.0 90-91 0.008502979585763178 92-93 0.003188617344661192 94-95 0.0 96-97 0.0 98-99 1.0901255879183562E-4 100-101 0.01120104041586111 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1834651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.75371540119008 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17367852854667 43.231392722708804 2 13.752726408296784 15.61036641133813 3 4.460011826800174 7.5936672571247685 4 1.8649577130006094 4.233731171155635 5 1.0348461352425273 2.936568152178792 6 0.5982479785594456 2.037167730870002 7 0.39411155526693964 1.5657106530758198 8 0.2765647538283306 1.2556861863018616 9 0.21666957088884312 1.1067122846091042 >10 1.1062504862405007 11.450214509653692 >50 0.08080488545821285 3.127516518477122 >100 0.03930042911994465 4.0392449185107395 >500 0.0012519196717358672 0.42433898780665025 >1k 2.8890453963135385E-4 0.23217310924815168 >5k 2.8890453963135385E-4 1.1555093869407775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8908 0.48554193685883584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7140 0.3891748348868531 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5094 0.2776549872428053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 5.450627939591781E-5 0.0 0.0 0.0 0.0 18-19 5.450627939591781E-5 0.0 0.0 0.0 0.0 20-21 5.450627939591781E-5 0.0 0.0 2.7253139697958904E-5 0.0 22-23 5.450627939591781E-5 0.0 0.0 5.450627939591781E-5 0.0 24-25 5.450627939591781E-5 0.0 0.0 1.362656984897945E-4 0.0 26-27 5.450627939591781E-5 0.0 0.0 3.8154395577142465E-4 0.0 28-29 5.450627939591781E-5 0.0 0.0 9.538598894285617E-4 0.0 30-31 5.450627939591781E-5 0.0 0.0 0.002207504315534671 0.0 32-33 5.450627939591781E-5 0.0 0.0 0.004415008631069342 0.0 34-35 8.175941909387671E-5 0.0 0.0 0.007249335159657068 0.0 36-37 1.0901255879183562E-4 0.0 0.0 0.011664343790726412 0.0 38-39 1.0901255879183562E-4 0.0 0.0 0.017278490568505944 0.0 40-41 1.0901255879183562E-4 0.0 0.0 0.023192421882963027 0.0 42-43 1.362656984897945E-4 0.0 0.0 0.029842187969265 0.0 44-45 2.72531396979589E-4 0.0 0.0 0.03651920719526493 2.7253139697958904E-5 46-47 2.72531396979589E-4 0.0 0.0 0.04567626213377912 5.450627939591781E-5 48-49 2.72531396979589E-4 0.0 0.0 0.05458803881501169 5.450627939591781E-5 50-51 2.72531396979589E-4 0.0 0.0 0.06379960003292179 5.450627939591781E-5 52-53 2.72531396979589E-4 0.0 0.0 0.08344911375515016 5.450627939591781E-5 54-55 2.72531396979589E-4 0.0 0.0 0.10865826797576214 5.450627939591781E-5 56-57 2.72531396979589E-4 0.0 0.0 0.14763025774384336 5.450627939591781E-5 58-59 2.72531396979589E-4 0.0 0.0 0.1970402000162429 8.175941909387671E-5 60-61 2.72531396979589E-4 0.0 0.0 0.25462608419803 1.0901255879183562E-4 62-63 2.72531396979589E-4 0.0 0.0 0.3055076960141193 1.0901255879183562E-4 64-65 2.72531396979589E-4 0.0 0.0 0.3662276912611717 1.0901255879183562E-4 66-67 3.2703767637550684E-4 0.0 0.0 0.4519115624715545 1.0901255879183562E-4 68-69 3.2703767637550684E-4 0.0 0.0 0.5633496506965086 1.0901255879183562E-4 70-71 4.087970954693835E-4 0.0 0.0 0.6836450093232991 1.0901255879183562E-4 72-73 4.905565145632602E-4 0.0 0.0 0.8135334731237712 1.0901255879183562E-4 74-75 4.905565145632602E-4 0.0 0.0 0.9510528160396718 1.0901255879183562E-4 76-77 4.905565145632602E-4 0.0 0.0 1.0954944564388542 1.0901255879183562E-4 78-79 4.905565145632602E-4 0.0 0.0 1.2448416619836689 1.0901255879183562E-4 80-81 5.45062793959178E-4 0.0 0.0 1.4122576991482303 1.0901255879183562E-4 82-83 5.995690733550959E-4 0.0 0.0 1.6036837523866936 1.0901255879183562E-4 84-85 5.995690733550959E-4 0.0 0.0 1.8431025846332627 1.0901255879183562E-4 86-87 5.995690733550959E-4 0.0 0.0 2.126616996911129 1.0901255879183562E-4 88-89 5.995690733550959E-4 0.0 0.0 2.4479042608103665 1.0901255879183562E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5570 0.0 37.884853 1 GTATCAA 9395 0.0 37.485153 1 ATCAACG 11915 0.0 29.300209 3 TCAACGC 12025 0.0 29.032183 4 TATCAAC 12065 0.0 28.975298 2 CAACGCA 12185 0.0 28.650963 5 AACGCAG 12500 0.0 27.966957 6 ACGCAGA 14120 0.0 24.660727 7 CGCAGAG 14195 0.0 24.363102 8 GGACCGT 160 1.8611681E-6 23.749115 6 GTACATG 7820 0.0 23.216381 1 GCAGAGT 15025 0.0 22.732698 9 GAGTACT 8065 0.0 22.353422 12-13 AGTACTT 8495 0.0 22.172588 12-13 TACATGG 7910 0.0 21.797544 2 AGAGTAC 12910 0.0 21.286963 10-11 GTACTTT 8445 0.0 21.063454 14-15 ACATGGG 8060 0.0 20.33113 3 TACTTTT 9325 0.0 19.83974 14-15 CAGAGTA 14710 0.0 18.456108 10-11 >>END_MODULE