FastQCFastQC Report
Thu 26 May 2016
SRR937279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195577
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78670.6580086435252602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57720.4827794445694422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.34903649033061024No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24540.2052565414021849No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21200.17732023951614995No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19660.1644394296645051No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15270.1277207574250759No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.10379925341487833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104400.028.0019321
GGTATCA73900.027.5947361
GTACATG81900.027.1628511
TACATGG84150.026.0910742
ACATGGG84400.025.1670763
GAGTACT60650.024.0839212-13
CATGGGG27650.023.8884144
TATCAAC125950.023.0540432
ATCAACG126550.022.8677163
TCAACGC126350.022.8663044
CAACGCA127700.022.586415
CATGGGA57550.022.3764744
AACGCAG130300.022.1656846
GTACTTT66450.021.90892414-15
AGTACTT62050.021.47355512-13
ATGGGAT24050.020.547915
ACGCAGA141850.020.261257
ATGGGGA15650.020.0391375
CGCAGAG143300.019.9899338
GCAGAGT142900.019.44759
Produced by FastQC (version 0.11.5)