##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937279_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1195577 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6236804488544 31.0 31.0 34.0 30.0 34.0 2 31.71925271228871 33.0 31.0 34.0 30.0 34.0 3 31.741696268830864 33.0 31.0 34.0 30.0 34.0 4 35.40212717374121 37.0 35.0 37.0 33.0 37.0 5 35.412505426250256 37.0 35.0 37.0 33.0 37.0 6 35.4586137070218 37.0 35.0 37.0 33.0 37.0 7 35.393640058314936 37.0 35.0 37.0 33.0 37.0 8 35.37326077701395 37.0 35.0 37.0 33.0 37.0 9 36.945061673150285 39.0 37.0 39.0 33.0 39.0 10-11 36.82585856034367 39.0 37.0 39.0 33.0 39.0 12-13 36.66440639122365 39.0 37.0 39.0 32.0 39.0 14-15 37.69419702787859 40.0 37.0 41.0 32.5 41.0 16-17 37.63060681160644 40.0 37.0 41.0 32.0 41.0 18-19 37.572963514687885 40.0 37.0 41.0 32.0 41.0 20-21 37.45934264376113 40.0 37.0 41.0 32.0 41.0 22-23 37.53371175591367 40.0 37.0 41.0 32.0 41.0 24-25 37.483656426980446 40.0 37.0 41.0 32.0 41.0 26-27 37.38427177839654 40.0 37.0 41.0 32.0 41.0 28-29 37.23926187941053 39.5 37.0 41.0 31.5 41.0 30-31 36.94814303051999 39.0 36.0 41.0 31.0 41.0 32-33 36.67687652070925 39.0 35.5 40.0 30.5 41.0 34-35 36.64012313719652 39.0 35.0 41.0 30.0 41.0 36-37 36.46602937326496 39.0 35.0 41.0 30.0 41.0 38-39 36.29510604503098 39.0 35.0 40.0 30.0 41.0 40-41 36.009933697285916 38.5 35.0 40.0 29.5 41.0 42-43 35.700812243795255 38.0 35.0 40.0 28.5 41.0 44-45 35.38581705737062 38.0 35.0 40.0 27.0 41.0 46-47 34.88801892308065 38.0 34.0 40.0 25.0 41.0 48-49 34.46309689798315 38.0 34.0 40.0 24.0 41.0 50-51 33.46431806567038 37.5 32.0 39.5 22.5 40.5 52-53 33.54461402318713 37.5 33.0 39.5 22.5 40.0 54-55 33.81680853679855 38.0 33.0 40.0 22.0 41.0 56-57 33.51818117946397 37.5 33.0 40.0 20.0 41.0 58-59 33.20359458236483 37.0 32.5 40.0 18.5 41.0 60-61 32.82914233043961 36.5 32.0 39.5 17.5 41.0 62-63 32.403471712821506 36.0 31.5 39.0 13.5 41.0 64-65 31.911286767811696 35.5 31.0 39.0 9.5 40.0 66-67 31.443613836666312 35.0 30.5 38.5 7.5 40.0 68-69 30.918012808878057 35.0 30.0 38.0 4.5 40.0 70-71 30.410898252475583 35.0 29.0 37.0 2.0 39.5 72-73 29.876816800590845 34.0 29.0 37.0 2.0 39.0 74-75 29.29545106672343 34.0 28.5 36.0 2.0 39.0 76-77 28.735314413040733 34.0 27.5 35.5 2.0 38.0 78-79 28.144512649540765 33.5 26.5 35.0 2.0 37.0 80-81 27.524797650004977 33.0 26.0 35.0 2.0 37.0 82-83 26.941212067478716 33.0 25.0 35.0 2.0 36.0 84-85 26.308610821385823 32.0 24.0 35.0 2.0 36.0 86-87 25.64155257252356 32.0 21.0 34.5 2.0 35.0 88-89 24.997039504774683 31.0 19.0 34.0 2.0 35.0 90-91 24.347785211659307 31.0 15.5 34.0 2.0 35.0 92-93 23.59440839025843 31.0 4.5 34.0 2.0 35.0 94-95 22.71360481173525 30.0 2.0 34.0 2.0 35.0 96-97 21.73811264351857 29.5 2.0 34.0 2.0 35.0 98-99 20.460735694982425 29.0 2.0 34.0 2.0 35.0 100-101 17.49233759097072 22.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25850763794428033 1101 2 0.3988883112602757 1101 3 0.3029070742801565 1101 4 0.14815147263570339 1101 5 0.19211585625051697 1101 6 0.2387795148794254 1101 7 0.35256011299576073 1101 8 0.36829962275322004 1101 9 0.35878455938369314 1101 10-11 0.4852475818991451 1101 12-13 0.5452437852675942 1101 14-15 0.5317827899484371 1101 16-17 0.5112247912141115 1101 18-19 0.649980238364904 1101 20-21 0.5322181297196593 1101 22-23 0.5927948484105698 1101 24-25 0.7211593933410683 1101 26-27 0.6210789412621764 1101 28-29 0.5438817037220645 1101 30-31 0.6296019600557585 1101 32-33 0.4488657211045819 1101 34-35 0.6157638778054348 1101 36-37 0.5164990969077579 1101 38-39 0.5486284057232282 1101 40-41 0.8330123751980025 1101 42-43 0.7249339116635767 1101 44-45 0.8763700213980457 1101 46-47 0.8157471333796735 1101 48-49 0.7985364007680928 1101 50-51 0.8314124306701629 1101 52-53 0.8070567343294215 1101 54-55 0.9799278143928163 1101 56-57 1.160883226150375 1101 58-59 1.0437117639458435 1101 60-61 0.9504325889092797 1101 62-63 1.1928063210383968 1101 64-65 0.7652381289330314 1101 66-67 1.0934249057376597 1101 68-69 1.1841036162890113 1101 70-71 1.139043307283309 1101 72-73 1.1899167597209264 1101 74-75 1.3731743961466485 1101 76-77 1.241961587555597 1101 78-79 1.410941753702712 1101 80-81 1.7091634815080674 1101 82-83 1.717284260623824 1101 84-85 1.83683214117249 1101 86-87 1.858218899599084 1101 88-89 1.7983876803570134 1101 90-91 1.8074703023841892 1101 92-93 2.2835210870450346 1101 94-95 2.4569304867546045 1101 96-97 2.6038519547748216 1101 98-99 2.858830291509779 1101 100-101 2.718571346805689 1102 1 0.26341220573676694 1102 2 0.3737004971554292 1102 3 0.34232565732644815 1102 4 0.37554137750062466 1102 5 0.2688798645461574 1102 6 0.31791155682702055 1102 7 0.2681421204534118 1102 8 0.1898891991926419 1102 9 0.4171917934707423 1102 10-11 0.44611183895867157 1102 12-13 0.4522672467039257 1102 14-15 0.619816396769096 1102 16-17 0.572955396544593 1102 18-19 0.620343710467921 1102 20-21 0.5601621303876456 1102 22-23 0.5777531708678509 1102 24-25 0.6265113901094708 1102 26-27 0.666247603481132 1102 28-29 0.7055124123343219 1102 30-31 0.615786048343999 1102 32-33 0.6679306487057843 1102 34-35 0.871452085704945 1102 36-37 1.0262085614447969 1102 38-39 0.9339153041332295 1102 40-41 1.0320851711702446 1102 42-43 1.1672845536579644 1102 44-45 1.3532271563029994 1102 46-47 0.9528068600327657 1102 48-49 1.2624558575345546 1102 50-51 1.2715665484384644 1102 52-53 1.2492215744457766 1102 54-55 1.4122985636106833 1102 56-57 1.5841414087672945 1102 58-59 1.5230038813575888 1102 60-61 1.5125844454612007 1102 62-63 1.5757849884252053 1102 64-65 1.7342163615284 1102 66-67 1.9210383746436825 1102 68-69 1.8800414757159736 1102 70-71 1.770380411316566 1102 72-73 1.977682872015972 1102 74-75 1.9990149239744284 1102 76-77 1.8524098966456712 1102 78-79 1.7363485432192718 1102 80-81 2.163212119474988 1102 82-83 1.9713743467019995 1102 84-85 2.312922373184122 1102 86-87 2.523609275362695 1102 88-89 2.1909174669556606 1102 90-91 2.105155000358348 1102 92-93 2.312574633340919 1102 94-95 2.3669568676123482 1102 96-97 2.065626039249235 1102 98-99 2.0181704466054526 1102 100-101 1.8943493263494275 1103 1 0.3773406495004714 1103 2 0.3451489004315924 1103 3 0.38355636573214724 1103 4 0.3457992329241222 1103 5 0.25567979528332074 1103 6 0.36223605469005093 1103 7 0.21964550296187468 1103 8 0.29660226898535313 1103 9 0.41661658495765863 1103 10-11 0.3139142080151345 1103 12-13 0.2936189421198705 1103 14-15 0.49614062114797974 1103 16-17 0.4735877543272622 1103 18-19 0.39568205420925295 1103 20-21 0.4055614655536175 1103 22-23 0.3997094148900331 1103 24-25 0.4592617117134594 1103 26-27 0.33116412021457364 1103 28-29 0.14362473257236985 1103 30-31 0.24579654924359318 1103 32-33 0.38789439719251817 1103 34-35 0.42833901858762147 1103 36-37 0.43887533661332867 1103 38-39 0.4667783636331251 1103 40-41 0.6087915301969602 1103 42-43 0.4075050799811848 1103 44-45 0.49978976522289287 1103 46-47 0.36189493159048425 1103 48-49 0.5378098077084132 1103 50-51 0.3578221862622115 1103 52-53 0.3409762502444309 1103 54-55 0.3986507383670528 1103 56-57 0.4574513621783751 1103 58-59 0.7539467551260657 1103 60-61 0.6924994870151835 1103 62-63 0.6702117084212773 1103 64-65 0.5243280515638453 1103 66-67 0.8515971815393435 1103 68-69 0.8141845599483482 1103 70-71 1.0781099057647623 1103 72-73 1.083133307101015 1103 74-75 0.8797698888636738 1103 76-77 0.8523645636749997 1103 78-79 0.962768524209153 1103 80-81 0.9291888331913292 1103 82-83 1.022471293117686 1103 84-85 1.0820259471314877 1103 86-87 1.3215137773725836 1103 88-89 1.5176087769224473 1103 90-91 1.4007973128154738 1103 92-93 1.5153200277712067 1103 94-95 1.7037786101968742 1103 96-97 2.0105951570919167 1103 98-99 2.154010693216815 1103 100-101 1.736169214741139 1104 1 0.24733342755436283 1104 2 0.3211472251122167 1104 3 0.2942373156439686 1104 4 0.40289922352913976 1104 5 0.36810476987741936 1104 6 0.4467005513304443 1104 7 0.48429863081040025 1104 8 0.456143449497425 1104 9 0.6145072316409781 1104 10-11 0.7303662602455034 1104 12-13 0.7274509985241977 1104 14-15 0.8872276495430711 1104 16-17 0.702695209283327 1104 18-19 0.712786806852229 1104 20-21 0.670637726292405 1104 22-23 0.6303053652931183 1104 24-25 0.598855762321584 1104 26-27 0.7576960585002794 1104 28-29 0.8342521956181699 1104 30-31 0.7288468497038068 1104 32-33 0.7586351881354716 1104 34-35 0.7352824858819602 1104 36-37 0.6025854518560863 1104 38-39 0.7301136835395212 1104 40-41 0.6766961115786856 1104 42-43 0.6879478777932277 1104 44-45 0.6578424502879514 1104 46-47 0.5510385608227963 1104 48-49 0.530070061688555 1104 50-51 0.7794755457580749 1104 52-53 0.6901630820620213 1104 54-55 1.0103438364958706 1104 56-57 1.0351572073648043 1104 58-59 1.0076984736552035 1104 60-61 0.9792860508964196 1104 62-63 1.0880396597193993 1104 64-65 1.2185736594085803 1104 66-67 1.320250923289148 1104 68-69 1.179244253958725 1104 70-71 1.1223810808765151 1104 72-73 1.1283798393835447 1104 74-75 1.141968128774284 1104 76-77 1.190373874032936 1104 78-79 1.5039135361536893 1104 80-81 1.4931690363094638 1104 82-83 1.6414781356785433 1104 84-85 1.339578689929084 1104 86-87 1.3206198194951035 1104 88-89 1.3212761916020028 1104 90-91 1.5285981477499 1104 92-93 1.828091254160931 1104 94-95 1.947348962494452 1104 96-97 1.6770265905585724 1104 98-99 1.6992037960072643 1104 100-101 1.0986879598867674 1105 1 0.23138612583945317 1105 2 0.019531439689831842 1105 3 0.18315582772134675 1105 4 0.22763203376253216 1105 5 0.052873483415261546 1105 6 0.14516269768084555 1105 7 0.1684008226073317 1105 8 0.060471534057505494 1105 9 0.03320114981808331 1105 10-11 0.16269413698752544 1105 12-13 0.11595763434629447 1105 14-15 0.2923669081521396 1105 16-17 0.3748502552288926 1105 18-19 0.2982588204588339 1105 20-21 0.2857405620518776 1105 22-23 0.2578355843634341 1105 24-25 0.22854927169868944 1105 26-27 0.2771328578833945 1105 28-29 0.11386866191813994 1105 30-31 0.21824471054500094 1105 32-33 0.3158357421346665 1105 34-35 0.4509668704055372 1105 36-37 0.4298462594206569 1105 38-39 0.6147801287158785 1105 40-41 0.47638274381865386 1105 42-43 0.6161211023502133 1105 44-45 0.5588479068792722 1105 46-47 0.6205506258917239 1105 48-49 0.688346757764208 1105 50-51 0.5371350768822438 1105 52-53 0.5374414174393536 1105 54-55 0.7889970934287263 1105 56-57 0.8032951438681906 1105 58-59 0.7647345505971046 1105 60-61 0.9003104025245818 1105 62-63 0.8178358476252114 1105 64-65 1.0028373203698244 1105 66-67 1.1091942504836503 1105 68-69 1.0076552387839044 1105 70-71 1.0961860928465654 1105 72-73 1.3283599462731246 1105 74-75 1.3039551292076936 1105 76-77 1.2751909873033505 1105 78-79 1.087761629354624 1105 80-81 1.115650096837843 1105 82-83 1.3256804766396186 1105 84-85 1.3707325997114381 1105 86-87 1.3894387019534769 1105 88-89 1.6234002425775849 1105 90-91 1.7862727183389957 1105 92-93 1.661038004884535 1105 94-95 1.613653314778876 1105 96-97 1.5204452523469847 1105 98-99 1.7593120938269529 1105 100-101 1.5568372306778002 1106 1 0.07866833111646088 1106 2 -0.631152085964171 1106 3 -0.08883030494617472 1106 4 -0.013917384776725328 1106 5 0.05743828760807901 1106 6 -0.034765378887520626 1106 7 0.20404059812074848 1106 8 0.19247689879196628 1106 9 0.07729010093939337 1106 10-11 -0.3459827867815122 1106 12-13 -0.09470027666884562 1106 14-15 -0.033988474577249406 1106 16-17 0.050141829109271896 1106 18-19 0.07197485505406576 1106 20-21 -0.05453065507928301 1106 22-23 -0.0369211468599957 1106 24-25 -0.004735137955584889 1106 26-27 0.21749447135258748 1106 28-29 0.43125540641282356 1106 30-31 0.2979263411093669 1106 32-33 0.26014006991206884 1106 34-35 0.2671762131412123 1106 36-37 0.12636058787286686 1106 38-39 0.11879805615138395 1106 40-41 0.28444165467633553 1106 42-43 0.13927268702754247 1106 44-45 0.3292257438903121 1106 46-47 0.44084678285238965 1106 48-49 0.5754581800374154 1106 50-51 0.41274170238553154 1106 52-53 0.54791361529346 1106 54-55 0.6626812151643335 1106 56-57 0.5221618296544506 1106 58-59 0.23217429574410176 1106 60-61 0.34752976617934905 1106 62-63 0.6396397817638118 1106 64-65 0.6380592764172413 1106 66-67 0.971299065248477 1106 68-69 1.0139717356235494 1106 70-71 1.0597491987691399 1106 72-73 1.092345370390813 1106 74-75 0.9515014942345736 1106 76-77 1.0024639701856835 1106 78-79 1.2253735352412107 1106 80-81 0.986067719484442 1106 82-83 1.0496757136101742 1106 84-85 0.9466395153827563 1106 86-87 0.8724124181286612 1106 88-89 1.4760619437719988 1106 90-91 1.4999180666530982 1106 92-93 1.6171493877938623 1106 94-95 1.7203174790274787 1106 96-97 1.829959220900868 1106 98-99 2.0913274401166255 1106 100-101 1.992027414450888 1107 1 0.305701119805466 1107 2 0.31235000458982753 1107 3 0.22373983864187608 1107 4 0.33231480425336457 1107 5 0.22551676590482828 1107 6 0.28884481531394357 1107 7 0.3748918111922066 1107 8 0.19264950703315264 1107 9 0.300953934355249 1107 10-11 0.3423348673968434 1107 12-13 0.3095368774269929 1107 14-15 0.33687202220440327 1107 16-17 0.4185531456081435 1107 18-19 0.43216389890068996 1107 20-21 0.35443603359321685 1107 22-23 0.4753608578712587 1107 24-25 0.5677919567245127 1107 26-27 0.47036588852813566 1107 28-29 0.49658178040193235 1107 30-31 0.4836689691558007 1107 32-33 0.5854007014794291 1107 34-35 0.6152001582366609 1107 36-37 0.6865004624396249 1107 38-39 0.7544956410113741 1107 40-41 0.6962711644964301 1107 42-43 0.6299587188259679 1107 44-45 0.8796087828956516 1107 46-47 0.8916854113389405 1107 48-49 0.8002030499279442 1107 50-51 0.6660566746031762 1107 52-53 0.8931321607079923 1107 54-55 1.0819046843836801 1107 56-57 1.181131612897012 1107 58-59 1.0417678067745229 1107 60-61 1.122944782408645 1107 62-63 1.1324638176333153 1107 64-65 1.0629279291102058 1107 66-67 1.2827149342037494 1107 68-69 1.3766078967062363 1107 70-71 1.5067495288566128 1107 72-73 1.4599335312534372 1107 74-75 1.4330953353524691 1107 76-77 1.4791061553647573 1107 78-79 1.3440310419804966 1107 80-81 1.3735378053071834 1107 82-83 1.3564449136981978 1107 84-85 1.140197652038971 1107 86-87 1.3652048206153182 1107 88-89 1.5751827420335403 1107 90-91 1.543188064452515 1107 92-93 1.6584849354639957 1107 94-95 1.739769602590222 1107 96-97 1.6549379027515592 1107 98-99 2.0222287852230814 1107 100-101 1.6213969473771108 1108 1 0.010628777455934824 1108 2 0.14552508903356198 1108 3 0.046619436503519296 1108 4 -0.01394573459074877 1108 5 0.02097475680884031 1108 6 0.184044155721935 1108 7 0.20112056727614913 1108 8 0.0076871410127736794 1108 9 0.03527000659121171 1108 10-11 0.12012217556993221 1108 12-13 0.39748934956720205 1108 14-15 0.31857831758460264 1108 16-17 0.2451715397143701 1108 18-19 0.23281185496334444 1108 20-21 0.1526384458914194 1108 22-23 0.1798925608420845 1108 24-25 0.10471215407017809 1108 26-27 0.05094781889862787 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-0.07153554008105445 2316 66-67 0.2170802898835369 2316 68-69 0.18018391454239335 2316 70-71 0.14960353375496638 2316 72-73 0.2809949876533331 2316 74-75 0.18201224272672434 2316 76-77 0.4610075073542994 2316 78-79 0.7276142352824202 2316 80-81 0.723587777300903 2316 82-83 0.8373449953374283 2316 84-85 1.2269545245609663 2316 86-87 1.4713021360392737 2316 88-89 1.4466631943807577 2316 90-91 1.481214738148207 2316 92-93 1.7209524902776003 2316 94-95 1.5829538253518258 2316 96-97 1.6450394209483186 2316 98-99 2.175849283625073 2316 100-101 2.799600371831538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4557.0 3 830.0 4 835.0 5 867.0 6 1111.0 7 1439.0 8 1782.0 9 2218.0 10 2965.0 11 3857.0 12 5142.0 13 6957.0 14 7795.0 15 10037.0 16 13005.0 17 16716.0 18 14426.0 19 10382.0 20 10412.0 21 11435.0 22 12724.0 23 14386.0 24 16032.0 25 18665.0 26 21458.0 27 25038.0 28 29327.0 29 34634.0 30 41758.0 31 50271.0 32 61934.0 33 76999.0 34 98524.0 35 128744.0 36 160957.0 37 175246.0 38 95147.0 39 6963.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.14091535246456 19.02098864240783 14.88592910271834 21.952166902409274 2 15.196765742768875 20.670717594533762 40.050767976761115 24.081748685936248 3 18.938847593618764 25.830778468020014 29.77896781205136 25.451406126309855 4 12.403417352759156 16.538420899923022 37.182746863317625 33.87541488400019 5 13.600914392286366 38.14216686820642 33.0686481853875 15.188270554119713 6 31.17534280796969 36.21931662236172 17.546604027768346 15.058736541900242 7 28.620889116165216 33.67558912538667 19.935868954705878 17.767652803742234 8 27.07100802540498 33.85693099727792 19.510628862116114 19.56143211520099 9 27.740701117777455 16.333032621126094 19.90150277851372 36.02476348258273 10-11 25.173879103400104 26.925205363903075 26.509446697617634 21.39146883507918 12-13 26.64468637207762 24.380688082432393 27.374856946429844 21.59976859906014 14-15 23.453540835676453 25.10231763484886 26.56662089409098 24.87752063538371 16-17 21.878770935437185 29.088914754382827 26.68700046566272 22.34531384451727 18-19 21.868274776169883 26.947373362005273 30.11974067851235 21.064611183312497 20-21 22.881539208216083 25.876483788036147 29.912186068968637 21.329790934779126 22-23 22.485903220696006 25.967956492984513 29.72109039122282 21.82504989509666 24-25 22.241734476406823 26.171297567231704 29.89448713623209 21.692480820129383 26-27 22.137009793957663 26.368163791148852 29.824317295897053 21.67050911899644 28-29 21.667531544280198 26.30144519791725 30.39593510316076 21.635088154641792 30-31 21.72398585738157 26.411222538309914 30.106010948204382 21.75878065610414 32-33 21.66780894413739 26.63074256419285 29.68056053337022 22.020887958299536 34-35 22.22692626262965 26.36285365534679 29.48195381060807 21.92826627141549 36-37 22.371198315447817 26.129796690243918 29.499823128727083 21.999181865581185 38-39 22.174195105760237 26.274185382993203 29.815409915210765 21.736209596035792 40-41 21.98689567456684 26.349751805668763 29.95417467265378 21.709177847110617 42-43 21.9857918229636 26.593893417716213 29.374295354051842 22.046019405268343 44-45 21.93004735093759 26.925659279451004 28.869445024211927 22.27484834539948 46-47 22.10017071934754 26.96439371997584 28.32034041597175 22.61509514470487 48-49 22.417577258482883 27.12426245835478 28.04594848819682 22.41221179496552 50-51 22.45535265117215 27.32904048026294 27.870803568434116 22.344803300130796 52-53 22.536567122251544 27.447374621918218 27.868159623255707 22.147898632574528 54-55 22.00567646655676 27.324544912285553 27.92149189378611 22.748286727371575 56-57 22.29531404011077 27.981780175275084 27.47674956583544 22.246156218778708 58-59 22.426284024248844 27.1455647217614 27.691402571966496 22.73674868202326 60-61 22.426797354123757 27.078117044614064 28.20012110520019 22.294964496061986 62-63 22.6076618185567 27.12291647633791 28.10220507563839 22.167216629467 64-65 22.408071042336434 27.191243073271625 28.09817097495483 22.302514909437114 66-67 22.158232078464405 27.543838696227013 27.93406542751241 22.363863797796174 68-69 22.027188990302818 27.55252990034615 28.018112024674597 22.40216908467644 70-71 21.957866302135432 27.700658058778988 27.79779747479425 22.543678164291332 72-73 22.27447614649333 27.549365919442998 27.46240797649495 22.713749957568716 74-75 22.0300519333349 27.85566287923237 27.415788538457154 22.69849664897558 76-77 22.334650507394223 27.512787541556367 27.694520678850388 22.458041272199022 78-79 22.186005301073124 27.49822710789746 27.721955194029 22.593812397000416 80-81 21.841816637253 28.236198382367057 27.48781578480847 22.434169195571478 82-83 22.015179472050068 28.00160419134801 27.216019786702894 22.767196549899026 84-85 22.06072450219962 27.642927088444445 27.1589646808006 23.13738372855533 86-87 22.37857090478635 27.432929320441236 27.43695198985952 22.751547784912894 88-89 22.38903169948144 27.082392166361725 27.44099249776425 23.08758363639259 90-91 21.990995963144044 27.394968618295636 27.83071270853213 22.78332271002819 92-93 22.07874501494469 27.324263703768274 27.710369753224047 22.886621528062992 94-95 21.94069274355464 27.28195818461679 27.448076501591828 23.329272570236746 96-97 22.189579489896072 27.082119708210296 27.33990416168294 23.388396640210686 98-99 21.999944677423276 27.119839598056334 27.300434282227286 23.579781442293104 100-101 22.51674194833913 26.28698555253982 28.110591353984777 23.085681145136274 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2249.0 1 1329.5 2 450.0 3 508.5 4 468.0 5 497.5 6 889.0 7 1797.5 8 2803.0 9 3129.0 10 2699.5 11 2369.5 12 2382.5 13 2388.5 14 2519.0 15 2641.0 16 2639.5 17 2636.5 18 2693.0 19 2798.5 20 3006.0 21 3283.5 22 3785.0 23 4374.5 24 4978.5 25 5830.5 26 7019.0 27 8365.5 28 9237.5 29 10398.5 30 12143.0 31 13703.0 32 15690.5 33 17998.0 34 20846.0 35 23119.5 36 25491.5 37 28462.5 38 30305.5 39 30842.0 40 31137.5 41 33502.5 42 36259.5 43 38357.5 44 40299.0 45 42179.0 46 43123.0 47 44372.5 48 51998.0 49 53546.5 50 47469.5 51 44327.0 52 41714.0 53 40769.0 54 39641.0 55 37035.5 56 33856.0 57 30029.5 58 26457.5 59 23694.5 60 20998.5 61 17859.0 62 14517.5 63 11137.5 64 8252.5 65 6400.5 66 4901.5 67 3793.0 68 2753.0 69 1843.5 70 1379.5 71 1080.0 72 856.5 73 705.5 74 538.0 75 405.0 76 283.0 77 158.0 78 107.5 79 69.0 80 40.0 81 27.0 82 16.5 83 10.0 84 6.5 85 2.0 86 3.0 87 3.0 88 3.0 89 3.0 90 1.5 91 1.0 92 1.5 93 1.0 94 1.5 95 2.0 96 0.5 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3010261990653885 2 0.2587035381242697 3 0.2576998386553104 4 0.25719798892083073 5 0.25853625487944315 6 0.22650151349515754 7 0.2264178718727443 8 0.22917804541238246 9 0.23862954874508294 10-11 0.24573908665021155 12-13 0.23770949089853685 14-15 0.22775613783135676 16-17 0.2219430450736339 18-19 0.2124915417409335 20-21 0.2174682182745235 22-23 0.2152935360917783 24-25 0.2170081893512505 26-27 0.21290974985299985 28-29 0.22834162918824968 30-31 0.24005145632610864 32-33 0.23290009760977334 34-35 0.229930820014102 36-37 0.21905740910037583 38-39 0.209187697655609 40-41 0.20571657032545793 42-43 0.21763550151935007 44-45 0.21495896960212518 46-47 0.22445229374603226 48-49 0.23110180272788786 50-51 0.24230978013126714 52-53 0.25460509862601904 54-55 0.23064177380461484 56-57 0.2075148652073434 58-59 0.19785425781860977 60-61 0.2006980729806612 62-63 0.2062602408711442 64-65 0.19848156998670935 66-67 0.2027891135409932 68-69 0.20467105004529193 70-71 0.20462922923408527 72-73 0.20672026979441727 74-75 0.2098568306349152 76-77 0.21905740910037583 78-79 0.2183882761210696 80-81 0.2122406168736936 82-83 0.20513107896856494 84-85 0.19258483560657322 86-87 0.19567957563586452 88-89 0.2062602408711442 90-91 0.20454558761167202 92-93 0.21002411387974176 94-95 0.2050474373461517 96-97 0.21127873821594093 98-99 0.2152935360917783 100-101 0.200028940001355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1195577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.98716157831268 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57185837937803 36.0407651404733 2 13.891915804853308 13.610510483293295 3 5.022653219028392 7.381365743771286 4 2.3939567662287087 4.690925876749627 5 1.3098405010021816 3.2082684132205954 6 0.8336127548925482 2.450179363059817 7 0.5550343159169279 1.903268900073156 8 0.37896128947514884 1.4851390335555874 9 0.289030339199319 1.2742898334652404 >10 1.5402043961285712 13.97409710247191 >50 0.13947035928540927 4.719141901708183 >100 0.06952336172966203 6.008466606058343 >500 0.002054876286241335 0.7048895529944756 >1k 0.0015411572146810013 1.4045782072283113 >5k 3.4247938104022257E-4 1.1441138418769075 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7867 0.6580086435252602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5772 0.4827794445694422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4173 0.34903649033061024 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2454 0.2052565414021849 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2120 0.17732023951614995 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1966 0.1644394296645051 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1527 0.1277207574250759 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1241 0.10379925341487833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.182081120663914E-5 0.0 16-17 0.0 0.0 0.0 1.6728324482655655E-4 0.0 18-19 0.0 0.0 0.0 1.6728324482655655E-4 0.0 20-21 0.0 0.0 0.0 1.6728324482655655E-4 0.0 22-23 0.0 0.0 0.0 3.345664896531131E-4 0.0 24-25 0.0 0.0 0.0 5.018497344796696E-4 0.0 26-27 0.0 0.0 0.0 6.691329793062262E-4 0.0 28-29 0.0 0.0 0.0 0.0012546243361991742 0.0 30-31 0.0 0.0 0.0 0.0038475146310108007 0.0 32-33 0.0 0.0 0.0 0.007193179527541931 0.0 34-35 0.0 0.0 0.0 0.009576965766320361 0.0 36-37 0.0 0.0 0.0 0.01112433578096601 0.0 38-39 0.0 0.0 0.0 0.012295318494751906 0.0 40-41 0.0 0.0 0.0 0.014470000677497142 0.0 42-43 0.0 0.0 0.0 0.01748109908437516 0.0 44-45 0.0 0.0 0.0 0.02028309343521998 0.0 46-47 0.0 0.0 0.0 0.02471609942312373 0.0 48-49 0.0 0.0 0.0 0.03203474138428558 0.0 50-51 0.0 0.0 0.0 0.039980695513547014 0.0 52-53 0.0 0.0 0.0 0.06147659247375953 0.0 54-55 0.0 0.0 0.0 0.08824191164600859 0.0 56-57 0.0 0.0 0.0 0.12466783820699126 0.0 58-59 0.0 0.0 0.0 0.18008041305578815 0.0 60-61 0.0 0.0 0.0 0.2594144919147826 0.0 62-63 0.0 0.0 0.0 0.31633261596701845 0.0 64-65 0.0 0.0 0.0 0.37839469979767093 0.0 66-67 0.0 0.0 0.0 0.4838667856608148 0.0 68-69 0.0 0.0 0.0 0.6245101737487422 0.0 70-71 0.0 0.0 0.0 0.7579185614979211 0.0 72-73 0.0 0.0 0.0 0.8802862550885472 0.0 74-75 0.0 0.0 0.0 1.0878011202958906 0.0 76-77 0.0 0.0 0.0 1.2578445386620853 0.0 78-79 0.0 0.0 0.0 1.3920893426353969 0.0 80-81 0.0 0.0 0.0 1.5515939165775188 0.0 82-83 0.0 0.0 0.0 1.820418091013795 0.0 84-85 0.0 0.0 0.0 2.1022485377353366 0.0 86-87 0.0 0.0 0.0 2.4468938428892493 0.0 88-89 0.0 0.0 0.0 2.8548140353988076 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10440 0.0 28.001932 1 GGTATCA 7390 0.0 27.594736 1 GTACATG 8190 0.0 27.162851 1 TACATGG 8415 0.0 26.091074 2 ACATGGG 8440 0.0 25.167076 3 GAGTACT 6065 0.0 24.08392 12-13 CATGGGG 2765 0.0 23.888414 4 TATCAAC 12595 0.0 23.054043 2 ATCAACG 12655 0.0 22.867716 3 TCAACGC 12635 0.0 22.866304 4 CAACGCA 12770 0.0 22.58641 5 CATGGGA 5755 0.0 22.376474 4 AACGCAG 13030 0.0 22.165684 6 GTACTTT 6645 0.0 21.908924 14-15 AGTACTT 6205 0.0 21.473555 12-13 ATGGGAT 2405 0.0 20.54791 5 ACGCAGA 14185 0.0 20.26125 7 ATGGGGA 1565 0.0 20.039137 5 CGCAGAG 14330 0.0 19.989933 8 GCAGAGT 14290 0.0 19.4475 9 >>END_MODULE