FastQCFastQC Report
Thu 26 May 2016
SRR937279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195577
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.8998165739220477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84910.7102010159111458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63700.5327971347725826No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26310.22006110856933517No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24740.20692937385045043No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.16117740639038722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.12889174013886182No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12280.10271191232350572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG902.41287E-531.6679
GTACGCT902.4135597E-531.6656743
GTATCAA116300.029.5381951
TACCGTA650.00584386329.231087
TACGCTC1153.8656817E-628.912144
TGTACGC1153.8656817E-628.912142
GGTATCA81750.028.4795881
GTATAGG2800.027.1510941
GTGTACG1858.940333E-925.6834641
TGCACCG1951.5705155E-824.3582135
TATCAAC142650.023.9740142
AACGTCG701.7809554E-523.74925822-23
ATACCGA1000.001653985523.7492566
ATCAACG144100.023.6998163
TCAACGC145050.023.5445924
CAACGCA147550.023.242245
GTACATG78850.023.019031
AACGCAG149900.022.9729336
GAGTACT81400.022.8165812-13
AGTACTT82800.022.48816112-13
Produced by FastQC (version 0.11.5)