##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937279_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1195577 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.157390950143736 33.0 31.0 34.0 31.0 34.0 2 32.341737085942604 34.0 31.0 34.0 31.0 34.0 3 32.39887016896444 34.0 31.0 34.0 31.0 34.0 4 36.062289589043615 37.0 35.0 37.0 35.0 37.0 5 35.99036197584932 37.0 35.0 37.0 35.0 37.0 6 36.03727154336358 37.0 35.0 37.0 35.0 37.0 7 35.97228953049448 37.0 35.0 37.0 35.0 37.0 8 35.95120765956521 37.0 35.0 37.0 35.0 37.0 9 37.62899336471009 39.0 37.0 39.0 35.0 39.0 10-11 37.54650055998066 39.0 37.0 39.0 35.0 39.0 12-13 37.426743739633665 39.0 37.0 39.0 34.5 39.0 14-15 38.595269480761175 40.0 38.0 41.0 34.0 41.0 16-17 38.55604908759536 40.0 38.0 41.0 34.0 41.0 18-19 38.59040697504217 40.0 38.0 41.0 34.0 41.0 20-21 38.510483222745165 40.0 38.0 41.0 34.0 41.0 22-23 38.4522397971858 40.0 38.0 41.0 34.0 41.0 24-25 38.46876403610976 40.0 38.0 41.0 34.0 41.0 26-27 38.29709002431462 40.0 38.0 41.0 34.0 41.0 28-29 38.125888169478 40.0 37.5 41.0 33.5 41.0 30-31 38.10768691602465 40.0 37.0 41.0 34.0 41.0 32-33 37.95848657175573 40.0 37.0 41.0 33.5 41.0 34-35 37.79827940818534 40.0 37.0 41.0 33.0 41.0 36-37 37.601218909363425 40.0 36.5 41.0 33.0 41.0 38-39 37.45720518210036 40.0 36.0 41.0 33.0 41.0 40-41 37.23002868071232 40.0 36.0 41.0 32.5 41.0 42-43 36.9782201397317 39.0 36.0 41.0 31.5 41.0 44-45 36.59242901126402 39.0 35.0 41.0 31.0 41.0 46-47 36.09882550433807 39.0 35.0 41.0 30.0 41.0 48-49 35.66341272874938 39.0 35.0 40.0 28.5 41.0 50-51 35.37520209907016 39.0 35.0 40.0 27.0 41.0 52-53 35.108416270972086 38.5 35.0 40.0 27.0 41.0 54-55 34.8731829903051 38.0 34.5 40.0 26.0 41.0 56-57 34.57272388143967 38.0 34.0 40.0 26.0 41.0 58-59 34.28232309587756 38.0 34.0 40.0 25.0 41.0 60-61 33.922031370626904 37.0 33.5 40.0 24.0 41.0 62-63 33.52364130457511 36.5 33.0 39.5 23.0 41.0 64-65 33.08200015557342 36.0 32.5 39.0 22.0 40.5 66-67 32.630616430393026 35.5 32.0 39.0 20.5 40.0 68-69 32.18223167558426 35.0 31.5 38.0 19.5 40.0 70-71 31.739125961774107 35.0 31.0 37.5 18.0 39.5 72-73 31.260218287906177 35.0 31.0 37.0 16.0 39.0 74-75 30.77922751943204 34.0 30.0 36.5 12.0 39.0 76-77 29.47259733166496 33.5 28.5 35.0 10.0 37.0 78-79 30.12737991781374 34.0 30.0 35.0 7.5 37.0 80-81 29.958658873497903 34.0 30.0 35.0 7.0 37.0 82-83 29.58626085981915 34.0 29.5 35.0 2.0 36.5 84-85 29.15940504041145 34.0 29.0 35.0 2.0 36.0 86-87 28.78642111716769 34.0 29.0 35.0 2.0 36.0 88-89 28.333504241048466 33.5 28.5 35.0 2.0 35.0 90-91 27.777174954018015 33.0 27.0 35.0 2.0 35.0 92-93 27.29011389479724 33.0 26.0 35.0 2.0 35.0 94-95 26.782528017852467 33.0 25.0 34.5 2.0 35.0 96-97 26.08073423961819 32.0 24.5 34.0 2.0 35.0 98-99 25.078653654260663 32.0 20.5 34.0 2.0 35.0 100-101 22.16429472965773 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24954007438515902 1101 2 0.3362107121017033 1101 3 0.2513316048604679 1101 4 0.13818498184246408 1101 5 0.12549666686542338 1101 6 0.20320355079596197 1101 7 0.19558070734790078 1101 8 0.19731446875092473 1101 9 0.2579196540412241 1101 10-11 0.22328224023755894 1101 12-13 0.27876173052536757 1101 14-15 0.44457887075814995 1101 16-17 0.37189595353328997 1101 18-19 0.19160746939130746 1101 20-21 0.3119660058185616 1101 22-23 0.37136505283938703 1101 24-25 0.20942356533142714 1101 26-27 0.23648049302110508 1101 28-29 0.19113990600013153 1101 30-31 0.3595723259551349 1101 32-33 0.5214350056202051 1101 34-35 0.5475711208099057 1101 36-37 0.5694073802141588 1101 38-39 0.40387249184785645 1101 40-41 0.499698101923407 1101 42-43 0.4804642848473719 1101 44-45 0.4843386082859098 1101 46-47 0.3577675472549444 1101 48-49 0.44409289773852123 1101 50-51 0.522180114082154 1101 52-53 0.5983398024113313 1101 54-55 0.6759866413307236 1101 56-57 0.7172867957108977 1101 58-59 0.7760305483939689 1101 60-61 0.83806861802546 1101 62-63 0.8338783864469477 1101 64-65 0.6971567239183045 1101 66-67 0.6164248880821148 1101 68-69 0.7705949844004536 1101 70-71 0.6542865880225577 1101 72-73 0.628186003266979 1101 74-75 0.8500212733481369 1101 76-77 0.6643991114680112 1101 78-79 0.7945420591047849 1101 80-81 0.7390174395976388 1101 82-83 0.7259723211296176 1101 84-85 0.7998655942575219 1101 86-87 0.7685084125055823 1101 88-89 1.1170612689456725 1101 90-91 1.2305653276570965 1101 92-93 1.2575504027409181 1101 94-95 1.5429604764728282 1101 96-97 2.0201266904959 1101 98-99 2.4642285181356804 1101 100-101 3.0585741460229414 1102 1 0.24632276818140753 1102 2 0.35526179859135 1102 3 0.3119962941538432 1102 4 0.12326591849456037 1102 5 0.22278313198845723 1102 6 0.18527178898042962 1102 7 0.188706790095182 1102 8 0.2174314712776777 1102 9 0.3514682668082969 1102 10-11 0.29771469824596153 1102 12-13 0.25512878476822465 1102 14-15 0.3524365600597932 1102 16-17 0.36675443192874013 1102 18-19 0.41225961141616096 1102 20-21 0.4696489886984594 1102 22-23 0.5307240137931686 1102 24-25 0.3884271256564844 1102 26-27 0.3233718320207686 1102 28-29 0.36912155599603835 1102 30-31 0.5641736680916196 1102 32-33 0.5926415067301178 1102 34-35 0.6006732223269395 1102 36-37 0.4824272775642626 1102 38-39 0.5576935037083288 1102 40-41 0.5286877083017529 1102 42-43 0.5496356159937079 1102 44-45 0.6021467780020089 1102 46-47 0.6281277095269289 1102 48-49 0.6659854741636408 1102 50-51 0.6686175699353996 1102 52-53 0.8075011890199093 1102 54-55 0.7759239802115303 1102 56-57 0.8306406098774275 1102 58-59 0.7403663668587726 1102 60-61 0.8882313639214559 1102 62-63 1.0039141852784255 1102 64-65 1.0298003837489134 1102 66-67 1.0866078239473609 1102 68-69 1.2153245674444904 1102 70-71 1.0506328354640218 1102 72-73 1.1143144262152411 1102 74-75 1.033300157453649 1102 76-77 1.0685309841628872 1102 78-79 1.0940140658327664 1102 80-81 1.1471816643863164 1102 82-83 1.1979971027371867 1102 84-85 1.2864442294486977 1102 86-87 1.252138289146675 1102 88-89 1.5708891261384998 1102 90-91 1.7374181823487476 1102 92-93 1.8664596404040878 1102 94-95 1.9436858560314612 1102 96-97 2.013681546807863 1102 98-99 2.555854419970604 1102 100-101 3.1117458292149394 1103 1 0.29560639577031367 1103 2 0.2886312496685335 1103 3 0.1290480309639932 1103 4 0.15397045974347634 1103 5 0.1483627437450039 1103 6 0.13063961401596913 1103 7 0.20910811322339384 1103 8 0.24127215486915077 1103 9 0.2955251515116899 1103 10-11 0.3216702005910079 1103 12-13 0.27205284609704705 1103 14-15 0.3667651230269229 1103 16-17 0.3544234923248908 1103 18-19 0.2938398593206202 1103 20-21 0.41070014881376693 1103 22-23 0.40544189036709355 1103 24-25 0.3352857365799409 1103 26-27 0.39337820093156495 1103 28-29 0.4294352155632595 1103 30-31 0.2891489393277027 1103 32-33 0.32989012240239646 1103 34-35 0.5797728649396348 1103 36-37 0.4526015270992261 1103 38-39 0.43236084516741613 1103 40-41 0.17988654242693514 1103 42-43 0.2976745934305285 1103 44-45 0.35855303276289874 1103 46-47 0.4098680348392989 1103 48-49 0.3666043063714568 1103 50-51 0.3137458733898555 1103 52-53 0.21166543111580438 1103 54-55 0.3545384114053718 1103 56-57 0.2744414747710522 1103 58-59 0.14864847765106504 1103 60-61 0.21245425647710903 1103 62-63 0.1464681705296087 1103 64-65 0.18168701565038958 1103 66-67 0.3098061913658938 1103 68-69 0.37791498256816425 1103 70-71 0.3877860289488524 1103 72-73 0.39404300805871983 1103 74-75 0.4952899478741237 1103 76-77 0.5641582104019065 1103 78-79 0.2792702231277744 1103 80-81 0.49884178006713853 1103 82-83 0.5191875168204341 1103 84-85 0.4941108522687223 1103 86-87 0.46175980343139145 1103 88-89 0.6959308361873333 1103 90-91 0.9566056966081931 1103 92-93 0.798546762942081 1103 94-95 0.916573023753795 1103 96-97 1.0983222904469407 1103 98-99 1.320438816956628 1103 100-101 2.116337836194731 1104 1 0.21433619386420588 1104 2 0.31819317803572034 1104 3 0.295791793877612 1104 4 0.16956685120881332 1104 5 0.17495341861327773 1104 6 0.2818687433447451 1104 7 0.18238260956974983 1104 8 0.26261024450303694 1104 9 0.2715772642715848 1104 10-11 0.1749495880667027 1104 12-13 0.31102015800551186 1104 14-15 0.4634646147654351 1104 16-17 0.4132651409366943 1104 18-19 0.4033570503493493 1104 20-21 0.33706641981532215 1104 22-23 0.2526195581303199 1104 24-25 0.30338115806691945 1104 26-27 0.4826768115496165 1104 28-29 0.32583041020797765 1104 30-31 0.4252245323771362 1104 32-33 0.554638591414367 1104 34-35 0.36039710787241575 1104 36-37 0.49643092278657264 1104 38-39 0.5791460033868461 1104 40-41 0.6214260720035654 1104 42-43 0.612032209571538 1104 44-45 0.8010983994218961 1104 46-47 0.8318003751729677 1104 48-49 0.8523199854531285 1104 50-51 0.7626002231601063 1104 52-53 0.5947021769800429 1104 54-55 0.5621008719159519 1104 56-57 0.5736122060235758 1104 58-59 0.5387228558070802 1104 60-61 0.8535755299493317 1104 62-63 0.8903494179253713 1104 64-65 0.7576504856556525 1104 66-67 0.7284058223079484 1104 68-69 0.8399015125987646 1104 70-71 0.9545728940433378 1104 72-73 0.9936337883516266 1104 74-75 0.7125450202692925 1104 76-77 0.649619579444785 1104 78-79 0.7382525096375048 1104 80-81 0.9018238628993487 1104 82-83 0.7345861833063729 1104 84-85 0.9093584101106273 1104 86-87 1.0346379892096813 1104 88-89 1.2342046404604012 1104 90-91 1.5363447311910576 1104 92-93 1.5002782370725285 1104 94-95 1.4811230219249971 1104 96-97 1.7044608729230681 1104 98-99 1.8908520743555393 1104 100-101 2.3065482623325835 1105 1 0.18141275431123205 1105 2 0.10397855940237122 1105 3 0.10724544019588222 1105 4 0.16564291808794707 1105 5 0.033242009538462014 1105 6 0.0944469314720493 1105 7 0.057126425957022775 1105 8 0.01589739300681714 1105 9 0.13264747259551513 1105 10-11 0.17350614124010377 1105 12-13 0.15877462917007534 1105 14-15 0.18453517856762858 1105 16-17 0.19509719386466884 1105 18-19 0.21105493210907156 1105 20-21 0.2477948904794829 1105 22-23 0.20675021553267214 1105 24-25 0.25618902616807304 1105 26-27 0.2141215298946193 1105 28-29 0.16034707598594622 1105 30-31 0.1348145155466014 1105 32-33 0.19148917706480972 1105 34-35 0.2890338213938506 1105 36-37 0.30240507678249173 1105 38-39 0.2975696213625554 1105 40-41 0.21627758463193203 1105 42-43 0.11220618883621825 1105 44-45 0.10484466177305762 1105 46-47 0.29702366337424024 1105 48-49 0.30666256258849245 1105 50-51 0.24154189601346587 1105 52-53 0.23026244490127112 1105 54-55 0.34838289743108675 1105 56-57 0.32444826355089873 1105 58-59 0.28220393137492294 1105 60-61 0.37260899822768323 1105 62-63 0.47201986496662585 1105 64-65 0.43644340452868136 1105 66-67 0.4968561695145084 1105 68-69 0.4401935516630928 1105 70-71 0.2894081230328567 1105 72-73 0.4392849219854753 1105 74-75 0.33577907946189356 1105 76-77 0.45665469932982816 1105 78-79 0.5046715036613705 1105 80-81 0.5243845642306155 1105 82-83 0.5574889641034382 1105 84-85 0.7979204185081166 1105 86-87 0.9961301228859156 1105 88-89 0.9995558324747194 1105 90-91 1.1580362198109668 1105 92-93 0.8814579190288612 1105 94-95 0.941139920814674 1105 96-97 1.0991931636113144 1105 98-99 1.051858775120774 1105 100-101 1.6025290336930667 1106 1 -0.09230538906326302 1106 2 0.1162966420440199 1106 3 0.05145416997972063 1106 4 0.08338225047654646 1106 5 0.024316249815584 1106 6 -0.012438654599890242 1106 7 0.045373496159690774 1106 8 -0.062058553188386156 1106 9 -0.032756738214928305 1106 10-11 -0.10990043702653196 1106 12-13 -0.2967378604266173 1106 14-15 -0.037024418346241816 1106 16-17 0.04169193709970642 1106 18-19 0.02532982132786543 1106 20-21 0.13640565481355793 1106 22-23 0.1914376663278219 1106 24-25 0.18796720508253628 1106 26-27 0.15342172446465696 1106 28-29 0.22580709915440167 1106 30-31 0.10281519597938171 1106 32-33 0.26708961681557497 1106 34-35 -0.15099109031859115 1106 36-37 0.34880173535144365 1106 38-39 0.4253728919386148 1106 40-41 0.21827530152690144 1106 42-43 0.22119352491377242 1106 44-45 0.34024136532930527 1106 46-47 0.42795847116603625 1106 48-49 0.5828374338123794 1106 50-51 0.5872586841458158 1106 52-53 0.6658539996620547 1106 54-55 0.4835190964548701 1106 56-57 0.45832692720340873 1106 58-59 0.8046385311005011 1106 60-61 0.7528739171380963 1106 62-63 0.767632760573612 1106 64-65 0.6743052710513879 1106 66-67 0.6369929871135298 1106 68-69 0.6213208207095917 1106 70-71 0.5540205575201185 1106 72-73 0.6401637205998689 1106 74-75 0.8271063732514001 1106 76-77 0.5541084186541205 1106 78-79 0.7208298139387139 1106 80-81 0.5611356664192471 1106 82-83 0.4735187794306057 1106 84-85 0.7290420641432256 1106 86-87 0.9104581533423115 1106 88-89 0.9024648231848431 1106 90-91 0.9860441825614927 1106 92-93 1.023085643571676 1106 94-95 0.8236935681593032 1106 96-97 1.222547612854406 1106 98-99 1.6453845415917456 1106 100-101 1.9036901275590523 1107 1 0.1036613546238172 1107 2 0.06819602037928263 1107 3 0.002497383931419961 1107 4 0.09343685244606803 1107 5 0.04263129080144523 1107 6 0.12952715383934787 1107 7 0.10637366120342762 1107 8 0.1011250029539923 1107 9 0.23914043953717368 1107 10-11 0.1578998738058317 1107 12-13 0.22010863514471168 1107 14-15 0.24185410946362396 1107 16-17 0.14298134129182216 1107 18-19 0.16055737537968895 1107 20-21 0.11751811836639092 1107 22-23 0.16288509989772137 1107 24-25 0.1382443410235581 1107 26-27 0.05263123373460843 1107 28-29 0.23662262151783153 1107 30-31 0.33641500617908804 1107 32-33 0.3056235470570883 1107 34-35 0.2208270039764031 1107 36-37 0.266370297270349 1107 38-39 0.20188441749288444 1107 40-41 0.33294425129859206 1107 42-43 0.5041335040082373 1107 44-45 0.35641943376744223 1107 46-47 0.36372791881967004 1107 48-49 0.2627677716018866 1107 50-51 0.2965214200208166 1107 52-53 0.32805657709507585 1107 54-55 0.22023582638829708 1107 56-57 0.20242420673249484 1107 58-59 0.456967023150888 1107 60-61 0.45022290211911553 1107 62-63 0.3944544308162321 1107 64-65 0.4540062668152629 1107 66-67 0.28478621481887956 1107 68-69 0.36713283589360657 1107 70-71 0.36846325983623274 1107 72-73 0.5431417285219631 1107 74-75 0.4083333358964971 1107 76-77 0.5257181452316608 1107 78-79 0.5217595603215059 1107 80-81 0.4499350420037729 1107 82-83 0.537586694928212 1107 84-85 0.7027822302872515 1107 86-87 0.8721662872477935 1107 88-89 1.1158347412131207 1107 90-91 1.0836015352599588 1107 92-93 0.9544800568411915 1107 94-95 0.9789839075992539 1107 96-97 1.3904043136301105 1107 98-99 1.3485024928488265 1107 100-101 2.0733898167266815 1108 1 0.007786464334181176 1108 2 0.052055963462876775 1108 3 -3.9763987240348797E-4 1108 4 0.03500045786110206 1108 5 0.0871451076582801 1108 6 0.04579753337071679 1108 7 0.03167486602270486 1108 8 0.07516021665648509 1108 9 0.03708586361760524 1108 10-11 -0.03162943548430519 1108 12-13 -0.028897322460544217 1108 14-15 -0.04672005474287033 1108 16-17 0.04722015083462594 1108 18-19 0.18532199677920147 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94-95 0.3154433277776896 2316 96-97 0.5676423450630459 2316 98-99 0.8601780007143809 2316 100-101 1.552702143517891 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 8.0 5 31.0 6 62.0 7 131.0 8 305.0 9 640.0 10 1140.0 11 1938.0 12 2945.0 13 4368.0 14 5715.0 15 8275.0 16 11690.0 17 17093.0 18 14478.0 19 7907.0 20 6530.0 21 6799.0 22 7112.0 23 7788.0 24 8793.0 25 10588.0 26 12786.0 27 16008.0 28 19726.0 29 24319.0 30 30664.0 31 38829.0 32 49296.0 33 65457.0 34 89132.0 35 128824.0 36 185793.0 37 247668.0 38 153532.0 39 9200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93979204356609 19.377051155676124 15.497072108634057 21.18608469212373 2 15.962431497453947 21.30571373128935 39.20545410747623 23.52640066378047 3 19.213317084554152 25.77717704505858 30.43576448861094 24.573741381776333 4 12.979757890959762 17.342086707924288 36.780483398392576 32.89767200272337 5 13.463486362537608 38.004059998310424 33.297506538738524 15.234947100413443 6 31.197154177439014 36.80733235918724 16.86541310179102 15.130100361582736 7 28.41356098352511 34.12159986349687 19.803576013924655 17.66126313905336 8 27.307484168731914 33.89468014188965 19.158029972138976 19.639805717239458 9 27.721008349943165 16.1988730127796 19.762591618942153 36.31752701833508 10-11 25.26809231024016 27.077678811151433 26.26815336862452 21.386075509983883 12-13 26.930661350136116 24.485525712108533 27.45390799708745 21.129904940667902 14-15 23.508230753853578 25.126654326739306 26.921394439672223 24.44372047973489 16-17 21.653310493594308 29.036231041580763 27.162073208166433 22.1483852566585 18-19 21.599612572004983 26.321140336423333 31.16838145932884 20.910865632242842 20-21 22.56379673537701 25.28018956576943 31.24945371451131 20.906559984342255 22-23 22.213839423873132 25.242394840954525 31.209381299296567 21.334384435875776 24-25 22.059367200412186 25.533633997336846 31.30206695350191 21.104931848749057 26-27 21.979871315265054 25.68295544128994 31.2558785336548 21.081294709790203 28-29 21.521557022212164 25.596540805670415 31.75451898872349 21.127383183393928 30-31 21.579416422815978 25.687525118677257 31.514932779178544 21.218125679328224 32-33 21.580754691572963 25.847657339630448 31.12206725720564 21.449520711590957 34-35 22.059892420145253 25.513204084722275 30.99904899475316 21.427854500379315 36-37 22.234536127744178 25.38556696891961 30.943469136659534 21.436427766676676 38-39 22.06505483549352 25.434309487644448 31.256774977082173 21.243860699779855 40-41 21.88123391467049 25.58864882813905 31.341896005025188 21.188221252165274 42-43 21.878136752365528 25.78163686612508 30.6734230918508 21.66680328965859 44-45 21.98875522028276 26.07172101838694 30.143646122332562 21.79587763899774 46-47 22.051653720337544 26.239924321060045 29.50320221951409 22.20521973908832 48-49 22.47203651458668 26.379271264000565 29.153872983505035 21.994819237907723 50-51 22.48880665988054 26.7095720309106 28.73198464005246 22.0696366691564 52-53 22.631415626095183 26.8897360855888 28.59272133873435 21.886126949581666 54-55 22.031747014203184 26.815462324885807 28.669546168920945 22.48324449199006 56-57 22.391615094636315 27.32513255106112 28.219386957092684 22.063865397209884 58-59 22.503945793537348 26.54283245662973 28.539734370935538 22.413487378897386 60-61 22.444225675134266 26.484617887430083 29.061741736416806 22.00941470101884 62-63 22.68804100446897 26.497038668358456 28.922520255909905 21.892400071262664 64-65 22.58729467027218 26.454381440927687 28.911981411485836 22.0463424773143 66-67 22.224750057921824 27.058901266919655 28.669253423242502 22.047095251916023 68-69 22.185522137009997 26.844025938939943 28.84398077246384 22.126471151586223 70-71 22.098074820776915 27.120628784260653 28.52710448595113 22.254191909011297 72-73 22.380756021793715 26.973152644671096 28.211720897242664 22.434370436292525 74-75 22.328256565658254 27.34976500886183 27.907236422246328 22.414742003233584 76-77 22.398975557408683 26.965431753872814 28.54717847533032 22.08841421338818 78-79 22.194597253041838 26.88450848418797 28.4834017382402 22.437492524529997 80-81 21.86132720853613 27.726946905134508 28.16058689653615 22.251138989793212 82-83 22.094803077687438 27.40464195829113 27.98273296569929 22.517821998322145 84-85 22.108506694681058 27.127133699572425 27.794176196243487 22.970183409503033 86-87 22.30149124648601 26.880953715235407 28.129304929753584 22.688250108525004 88-89 22.329427548372042 26.559017110566696 28.256314733388148 22.855240607673117 90-91 21.928489872395954 26.929179963111515 28.51697414041882 22.62535602407371 92-93 21.87391133971554 26.74420890232932 28.417436631345804 22.964443126609336 94-95 21.66932786428645 26.7234565402312 28.222523517933183 23.384692077549165 96-97 21.975539843941462 26.534593756822023 28.176060596682607 23.313805802553915 98-99 21.81453351505908 26.54589906358826 28.060610192003928 23.578957229348735 100-101 21.586445702632087 26.540820948615114 28.692380371213222 23.18035297753958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 900.0 1 622.5 2 473.5 3 612.5 4 495.0 5 445.0 6 924.5 7 2291.0 8 3728.0 9 4231.0 10 3554.0 11 2669.5 12 2529.0 13 2615.5 14 2721.0 15 2846.5 16 2929.0 17 2998.0 18 3073.0 19 3157.0 20 3312.0 21 3627.5 22 4090.5 23 4559.0 24 5114.0 25 5906.0 26 7005.5 27 8221.0 28 9237.0 29 10464.0 30 12134.5 31 13671.0 32 15428.0 33 17733.5 34 20732.0 35 22758.5 36 25006.0 37 28166.5 38 30340.5 39 30797.5 40 30942.5 41 33429.0 42 36264.0 43 38503.0 44 40646.0 45 42806.0 46 43207.5 47 44098.5 48 53595.5 49 54702.0 50 47118.5 51 44406.0 52 41546.0 53 40665.0 54 39726.0 55 36777.0 56 33194.5 57 29464.0 58 26111.5 59 23332.5 60 20619.0 61 17128.0 62 13857.0 63 10858.5 64 7826.5 65 5952.5 66 4519.0 67 3494.0 68 2519.5 69 1626.5 70 1193.5 71 908.0 72 708.5 73 630.0 74 539.0 75 398.0 76 249.0 77 137.0 78 83.0 79 58.5 80 35.0 81 14.0 82 7.0 83 5.5 84 2.5 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04341000203249143 2 8.364162241327827E-5 3 0.0 4 0.0 5 8.364162241327828E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0050603181560033355 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.091040560331957E-4 22-23 5.85491356892948E-4 24-25 8.364162241327827E-5 26-27 0.0016310116370589264 28-29 0.003220202462911214 30-31 2.091040560331957E-4 32-33 2.091040560331957E-4 34-35 0.0 36-37 0.0 38-39 8.364162241327827E-5 40-41 0.0 42-43 0.006105838436169315 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.509248672398348E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.345664896531131E-4 84-85 8.364162241327827E-5 86-87 0.0 88-89 0.0 90-91 0.006858613037888818 92-93 0.0011291619025792568 94-95 0.0 96-97 0.0 98-99 2.091040560331957E-4 100-101 0.009911532255973476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1195577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.579778591436906 #Duplication Level Percentage of deduplicated Percentage of total 1 71.89360679007865 33.487882864216886 2 14.561944060854131 13.5658426043095 3 5.412937538000811 7.564002961480662 4 2.5732174984552927 4.794396053826346 5 1.4161884041216732 3.2982871153873954 6 0.8955237097447046 2.5027977673974333 7 0.584683788448397 1.9064108988350204 8 0.4212147004711678 1.5696069989924333 9 0.3094728119330714 1.2973657553920674 >10 1.6955143888522206 14.402073127365961 >50 0.15564550321824075 5.008782288556154 >100 0.07389305363991881 6.099034409031068 >500 0.004527758957187113 1.4255288642425228 >1k 0.001086662149724907 0.916748855942922 >5k 3.62220716574969E-4 1.2536859067054167 >10k+ 1.811103582874845E-4 0.9075535283182069 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10758 0.8998165739220477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8491 0.7102010159111458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6370 0.5327971347725826 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2631 0.22006110856933517 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2474 0.20692937385045043 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1927 0.16117740639038722 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1541 0.12889174013886182 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1228 0.10271191232350572 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.182081120663914E-5 0.0 16-17 0.0 0.0 0.0 1.6728324482655655E-4 0.0 18-19 0.0 0.0 0.0 1.6728324482655655E-4 0.0 20-21 0.0 0.0 0.0 1.6728324482655655E-4 0.0 22-23 0.0 0.0 0.0 3.345664896531131E-4 0.0 24-25 0.0 0.0 0.0 5.018497344796696E-4 0.0 26-27 0.0 0.0 0.0 6.691329793062262E-4 0.0 28-29 0.0 0.0 0.0 0.0012546243361991742 0.0 30-31 0.0 0.0 0.0 0.0038475146310108007 0.0 32-33 0.0 0.0 0.0 0.0073604627723684885 0.0 34-35 0.0 0.0 0.0 0.009618786577527003 0.0 36-37 0.0 0.0 0.0 0.011082514969759372 0.0 38-39 0.0 0.0 0.0 0.012211676872338628 0.0 40-41 0.0 0.0 0.0 0.014386359055083862 0.0 42-43 0.0 0.0 0.0 0.017648382329201715 0.0 44-45 0.0 0.0 0.0 0.020408555868839898 0.0 46-47 0.0 0.0 0.0 0.024841561856743646 0.0 48-49 0.0 0.0 0.0 0.032285666251525416 0.0 50-51 0.0 0.0 0.0 0.04027344119199349 0.0 52-53 0.0 0.0 0.0 0.06202026301944584 0.0 54-55 0.0 0.0 0.0 0.08941289435979448 0.0 56-57 0.0 0.0 0.0 0.12625702903284355 0.0 58-59 0.0 0.0 0.0 0.18183688712646695 0.0 60-61 0.0 0.0 0.0 0.2608363994958083 0.0 62-63 0.0 0.0 0.0 0.3178381651704575 0.0 64-65 0.0 0.0 0.0 0.3796493241338701 0.0 66-67 0.0 0.0 0.0 0.4854141556754604 0.0 68-69 0.0 0.0 0.0 0.6274794513444135 0.0 70-71 0.0 0.0 0.0 0.7620588218073783 0.0 72-73 0.0 0.0 0.0 0.8861829894686833 0.0 74-75 0.0 0.0 0.0 1.0990509185104766 0.0 76-77 0.0 0.0 0.0 1.2710599150033834 0.0 78-79 0.0 0.0 0.0 1.4078139676490933 0.0 80-81 0.0 0.0 0.0 1.570998772977399 0.0 82-83 0.0 0.0 0.0 1.8441304909679594 0.0 84-85 0.0 0.0 0.0 2.1318158512584304 0.0 86-87 0.0 0.0 0.0 2.4839470816183313 0.0 88-89 0.0 0.0 0.0 2.9053335753364276 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATG 90 2.41287E-5 31.667 9 GTACGCT 90 2.4135597E-5 31.665674 3 GTATCAA 11630 0.0 29.538195 1 TACCGTA 65 0.005843863 29.23108 7 TACGCTC 115 3.8656817E-6 28.91214 4 TGTACGC 115 3.8656817E-6 28.91214 2 GGTATCA 8175 0.0 28.479588 1 GTATAGG 280 0.0 27.151094 1 GTGTACG 185 8.940333E-9 25.683464 1 TGCACCG 195 1.5705155E-8 24.358213 5 TATCAAC 14265 0.0 23.974014 2 AACGTCG 70 1.7809554E-5 23.749258 22-23 ATACCGA 100 0.0016539855 23.749256 6 ATCAACG 14410 0.0 23.699816 3 TCAACGC 14505 0.0 23.544592 4 CAACGCA 14755 0.0 23.24224 5 GTACATG 7885 0.0 23.01903 1 AACGCAG 14990 0.0 22.972933 6 GAGTACT 8140 0.0 22.81658 12-13 AGTACTT 8280 0.0 22.488161 12-13 >>END_MODULE