FastQCFastQC Report
Thu 26 May 2016
SRR937278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164793
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78770.6762574981133986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.49931618751142903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41530.3565440382969335No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23550.20218184690326949No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21180.18183488396650735No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16270.13968147130005074No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14870.12766216829943175No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.10963321379850324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73900.027.7434231
GTATCAA101700.027.5499971
TACATGG76300.026.4829962
ACATGGG77700.025.2103523
ATAGCGT950.001219539225.024644
GGACCGT1151.2818084E-424.7824866
GGTATCA73200.024.4995161
GAGTACT56250.023.22160512-13
AGTACTT57750.022.94744512-13
TATCAAC123300.022.5191762
ATCAACG124200.022.317713
GTATAGG3200.022.2981321
TCAACGC124000.022.2779834
CATGGGG27800.022.2341214
GTATAGC3007.2759576E-1222.199031
CAACGCA125500.021.8961685
AACGCAG127250.021.6875156
GTACTTT61850.021.15520914-15
CATGGGA51750.021.1319164
ATGGGAG17150.020.7912755
Produced by FastQC (version 0.11.5)