##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937278_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1164793 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65124790413404 31.0 31.0 34.0 30.0 34.0 2 31.743841180364235 33.0 31.0 34.0 30.0 34.0 3 31.76794331696705 33.0 31.0 34.0 30.0 34.0 4 35.41830522676561 37.0 35.0 37.0 33.0 37.0 5 35.4333576867306 37.0 35.0 37.0 33.0 37.0 6 35.49046482937312 37.0 35.0 37.0 33.0 37.0 7 35.42907022964595 37.0 35.0 37.0 33.0 37.0 8 35.42138817798527 37.0 35.0 37.0 33.0 37.0 9 37.02124068396702 39.0 37.0 39.0 33.0 39.0 10-11 36.89676191391946 39.0 37.0 39.0 33.0 39.0 12-13 36.75304496163696 39.0 37.0 39.0 32.5 39.0 14-15 37.86931712330002 40.0 38.0 41.0 33.0 41.0 16-17 37.84448009217088 40.0 37.5 41.0 33.0 41.0 18-19 37.84617953576301 40.0 38.0 41.0 33.0 41.0 20-21 37.807650801472874 40.0 37.5 41.0 33.0 41.0 22-23 37.65635095677945 40.0 37.0 41.0 32.0 41.0 24-25 37.487541563179036 40.0 37.0 41.0 32.0 41.0 26-27 37.31103165970262 39.5 37.0 41.0 32.0 41.0 28-29 37.36262709339771 39.5 37.0 41.0 32.0 41.0 30-31 37.17407771166207 39.0 36.5 41.0 31.5 41.0 32-33 36.95741818503374 39.0 36.0 41.0 31.0 41.0 34-35 36.816302982590045 39.0 35.5 41.0 31.0 41.0 36-37 36.791767292557566 39.0 36.0 41.0 31.0 41.0 38-39 36.68391679895055 39.0 35.0 41.0 30.0 41.0 40-41 36.48357476392801 39.0 35.0 41.0 30.0 41.0 42-43 36.204924394291524 39.0 35.0 41.0 30.0 41.0 44-45 35.92733386962318 39.0 35.0 41.0 29.5 41.0 46-47 35.49982829567142 39.0 35.0 40.0 27.0 41.0 48-49 35.11712038104625 39.0 35.0 40.0 26.0 41.0 50-51 34.21389637472066 37.5 34.0 39.5 24.0 40.5 52-53 34.28552798651778 38.0 33.5 39.5 24.0 40.5 54-55 34.5787513317817 38.0 34.0 40.0 24.0 41.0 56-57 34.3745957436214 38.0 34.0 40.0 23.5 41.0 58-59 34.12502221424751 38.0 34.0 40.0 23.0 41.0 60-61 33.827568074327374 37.5 33.5 40.0 22.0 41.0 62-63 33.477332882323296 37.0 33.0 40.0 21.5 41.0 64-65 33.08345989373219 36.0 33.0 39.0 20.0 41.0 66-67 32.69730930731898 36.0 32.0 39.0 19.0 41.0 68-69 32.224795736238114 35.0 32.0 39.0 17.5 40.0 70-71 31.782712894050704 35.0 31.0 38.0 13.5 40.0 72-73 31.38615659606471 35.0 31.0 37.0 10.0 39.5 74-75 30.94492068547802 35.0 31.0 37.0 7.5 39.0 76-77 30.502824106944324 34.5 30.0 36.0 6.5 39.0 78-79 30.040244489793466 34.0 30.0 36.0 2.0 37.5 80-81 29.56920027850442 34.0 29.0 35.0 2.0 37.0 82-83 29.108290485949006 34.0 29.0 35.0 2.0 37.0 84-85 28.68870005228397 34.0 29.0 35.0 2.0 36.0 86-87 28.250367661893574 34.0 27.5 35.0 2.0 36.0 88-89 27.833185810697692 33.5 27.0 35.0 2.0 35.5 90-91 27.400154362191394 33.0 26.0 35.0 2.0 35.0 92-93 26.954512947794157 33.0 25.5 35.0 2.0 35.0 94-95 26.41223161540291 33.0 25.0 35.0 2.0 35.0 96-97 25.74407341046864 32.0 23.5 34.5 2.0 35.0 98-99 24.93649687111787 32.0 19.0 34.0 2.0 35.0 100-101 23.025618715085 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04855725717917991 1101 2 0.10942053026277421 1101 3 0.07533984964978657 1101 4 -0.06414863910608659 1101 5 0.11009478750614932 1101 6 0.0806736243188908 1101 7 0.07453772016086901 1101 8 0.2194262055251457 1101 9 0.3006610700881325 1101 10-11 0.19867647922000486 1101 12-13 0.13217232720725036 1101 14-15 0.1743450632855854 1101 16-17 0.4126011179452007 1101 18-19 0.4102169127993136 1101 20-21 0.32332189454852767 1101 22-23 0.39918291259529326 1101 24-25 0.4080647033489413 1101 26-27 0.3371854858491261 1101 28-29 0.3019869570600875 1101 30-31 0.1869697429211996 1101 32-33 0.2537679362782299 1101 34-35 0.26023886974096655 1101 36-37 0.3126102593011879 1101 38-39 0.41421182023130854 1101 40-41 0.3523589155299547 1101 42-43 0.460213078409204 1101 44-45 0.548467209658007 1101 46-47 0.552266677074563 1101 48-49 0.5131950186985677 1101 50-51 0.6014615028784718 1101 52-53 0.5850496258465512 1101 54-55 0.8277737434837391 1101 56-57 0.9220884331375245 1101 58-59 0.849496177789014 1101 60-61 0.8541945041922077 1101 62-63 0.717715760895679 1101 64-65 0.753457669672251 1101 66-67 0.7412235829571685 1101 68-69 0.9811184887359943 1101 70-71 0.9001230670915028 1101 72-73 0.8152063089805601 1101 74-75 0.7022196192015784 1101 76-77 0.8284515532150536 1101 78-79 0.7411802834102623 1101 80-81 0.8570252640723304 1101 82-83 0.7619897074920807 1101 84-85 0.8097555120128028 1101 86-87 0.98428458084733 1101 88-89 0.7850881973342823 1101 90-91 0.7998930437865361 1101 92-93 0.9531910107707162 1101 94-95 1.017072910507629 1101 96-97 1.10090574859791 1101 98-99 1.502968061249252 1101 100-101 2.0736204985070543 1102 1 0.23765545263811916 1102 2 0.24863464039919236 1102 3 0.3229531996764763 1102 4 0.13545211826253478 1102 5 0.13320028665195593 1102 6 0.10455280168445569 1102 7 0.19695362462771016 1102 8 0.13153183692949 1102 9 0.20586390192858772 1102 10-11 0.3267541458229388 1102 12-13 0.22295411225901773 1102 14-15 0.4646333800591904 1102 16-17 0.5639737629036503 1102 18-19 0.5429688567600621 1102 20-21 0.4740647695477591 1102 22-23 0.5604067632651137 1102 24-25 0.5277816426532382 1102 26-27 0.343159391855103 1102 28-29 0.48039071292863156 1102 30-31 0.36915554493221947 1102 32-33 0.42672887614993016 1102 34-35 0.2915649569507437 1102 36-37 0.36624486800434397 1102 38-39 0.5989403441505701 1102 40-41 0.7364315604198666 1102 42-43 0.675033162246045 1102 44-45 0.6321032642965392 1102 46-47 0.5741630041931174 1102 48-49 0.4592568320934092 1102 50-51 0.6431736548346265 1102 52-53 0.74741035177464 1102 54-55 0.6282384301097963 1102 56-57 0.8075066202634886 1102 58-59 0.675054696027324 1102 60-61 0.6599769436839225 1102 62-63 0.39689456553884384 1102 64-65 0.5432608100246057 1102 66-67 0.5858287021166362 1102 68-69 0.596592532825376 1102 70-71 0.5545414275926568 1102 72-73 0.542390853507488 1102 74-75 0.7545525434242819 1102 76-77 0.7795648891935585 1102 78-79 0.4951026401067615 1102 80-81 0.5275887608511809 1102 82-83 0.6716972105261476 1102 84-85 0.7742512883053543 1102 86-87 0.5728467522996326 1102 88-89 0.5471554189386119 1102 90-91 0.7437794439762193 1102 92-93 0.8405111854068181 1102 94-95 0.8357248601561871 1102 96-97 1.1719912897238096 1102 98-99 1.1604126068863287 1102 100-101 1.5802450754339006 1103 1 0.22701145343107143 1103 2 0.1296393639259108 1103 3 0.3865355606195422 1103 4 0.09864142162778933 1103 5 0.0261293517217851 1103 6 0.13051237485810674 1103 7 -0.014304227979117456 1103 8 0.14761096796463846 1103 9 0.13709523169364246 1103 10-11 0.19765899854597535 1103 12-13 0.25273659761335665 1103 14-15 0.3664424702847029 1103 16-17 0.2516625159882935 1103 18-19 0.17694885525619242 1103 20-21 0.11019974893787321 1103 22-23 -0.06620596906608966 1103 24-25 0.13273963676078893 1103 26-27 0.1289901788589063 1103 28-29 0.019784652322577756 1103 30-31 0.06952672800134962 1103 32-33 -0.07481498565785927 1103 34-35 0.20594949714173794 1103 36-37 0.3161361790393471 1103 38-39 0.16153014926236864 1103 40-41 0.13997523082571206 1103 42-43 0.24953227017623902 1103 44-45 0.1936523213001422 1103 46-47 0.11917493263612755 1103 48-49 0.054668653898197306 1103 50-51 0.01149868269045129 1103 52-53 -0.2137593192190721 1103 54-55 -0.28140583569282995 1103 56-57 -0.2703033996286379 1103 58-59 -0.0309580595455472 1103 60-61 -0.09329602967702755 1103 62-63 -0.2963827827238461 1103 64-65 -6.266319566776701E-4 1103 66-67 -0.08754386861663477 1103 68-69 0.14674838869970586 1103 70-71 0.2592451305368293 1103 72-73 0.3317345498893083 1103 74-75 0.19160454323288434 1103 76-77 0.2553887720555643 1103 78-79 0.3665837494177815 1103 80-81 0.32712187680658644 1103 82-83 0.21777859736999972 1103 84-85 0.27968368581986525 1103 86-87 0.25730730640827915 1103 88-89 0.49521952492060706 1103 90-91 0.5226103882030593 1103 92-93 0.7097241815992845 1103 94-95 0.6558128366957767 1103 96-97 0.7291401525911461 1103 98-99 0.8356339153920977 1103 100-101 1.2951193995457615 1104 1 0.12421868115251655 1104 2 0.13793195537748204 1104 3 0.16198595375079705 1104 4 0.09694030853725621 1104 5 0.09519722211571491 1104 6 0.11207070160576649 1104 7 0.12033284557058721 1104 8 0.21694003288821762 1104 9 0.23402251245767047 1104 10-11 0.2751915808892207 1104 12-13 0.2427435628008041 1104 14-15 0.37729543785639663 1104 16-17 0.30796467117803417 1104 18-19 0.34434240174212505 1104 20-21 0.3464984215338731 1104 22-23 0.34036206876023556 1104 24-25 0.29397234099482006 1104 26-27 0.15586709595978476 1104 28-29 0.08425348980600234 1104 30-31 0.09543454642427207 1104 32-33 0.11659774188276373 1104 34-35 0.3625798198174337 1104 36-37 0.19948000480250982 1104 38-39 0.10691571257191157 1104 40-41 0.08991294597633726 1104 42-43 0.19456153735815462 1104 44-45 0.1372564039715627 1104 46-47 0.2914327249395967 1104 48-49 0.3743359322511921 1104 50-51 0.25567940251578136 1104 52-53 0.23129807660448876 1104 54-55 0.3796654637164707 1104 56-57 0.3838817404118089 1104 58-59 0.38300781103591675 1104 60-61 0.19238582199127308 1104 62-63 0.02400981274610814 1104 64-65 0.024950340062019905 1104 66-67 0.16077176153190464 1104 68-69 0.4176740889019612 1104 70-71 0.315308862002059 1104 72-73 0.4331095046825624 1104 74-75 0.3735339001585558 1104 76-77 0.45636590748827643 1104 78-79 0.5370828318882772 1104 80-81 0.47278872653266646 1104 82-83 0.509997009575148 1104 84-85 0.779786154443034 1104 86-87 0.9066650357571682 1104 88-89 0.6633946458067932 1104 90-91 0.8605833847070734 1104 92-93 1.0914780693794022 1104 94-95 1.2369900873219741 1104 96-97 1.4262482603372355 1104 98-99 1.6749544077772676 1104 100-101 2.09114321512903 1105 1 -0.0016153087477057682 1105 2 0.10495280400036222 1105 3 0.15055205022178697 1105 4 0.15596834962013162 1105 5 0.1903745722931518 1105 6 0.12546980730382273 1105 7 0.1752691789326235 1105 8 0.19711385495580913 1105 9 0.36056276696998424 1105 10-11 0.3190882721776873 1105 12-13 0.25738232676705053 1105 14-15 0.33682049900387767 1105 16-17 0.27997403577123237 1105 18-19 0.24362032811323076 1105 20-21 0.23307579510351673 1105 22-23 0.17541618572381878 1105 24-25 0.15819955641002537 1105 26-27 0.2785068290316062 1105 28-29 0.3437266896666742 1105 30-31 0.22034891424996772 1105 32-33 0.31599153677940706 1105 34-35 0.24245731921656244 1105 36-37 0.24543700941796942 1105 38-39 0.21920246757198925 1105 40-41 0.10040280699836757 1105 42-43 0.26185621614592236 1105 44-45 0.0390554186713743 1105 46-47 0.1551705677414148 1105 48-49 0.2874184720275821 1105 50-51 -0.009870950487993468 1105 52-53 -0.006590995509050401 1105 54-55 0.049874271266752146 1105 56-57 0.04556653175206549 1105 58-59 0.1692477327877029 1105 60-61 0.1049131182509555 1105 62-63 0.03288921051253624 1105 64-65 0.15753690085540484 1105 66-67 0.017151342451576568 1105 68-69 -0.018549059868590234 1105 70-71 0.022786462681725794 1105 72-73 0.25770043030692236 1105 74-75 0.08271418335845482 1105 76-77 -0.13293415150635113 1105 78-79 -0.1400350301157154 1105 80-81 -0.21854632565636933 1105 82-83 -0.05872240865303269 1105 84-85 0.08377266151146401 1105 86-87 -0.03955763745760166 1105 88-89 0.031448478722193585 1105 90-91 0.004321086127443863 1105 92-93 0.4978321373735959 1105 94-95 0.5787347601331625 1105 96-97 0.4041930753010483 1105 98-99 0.20277956262209074 1105 100-101 0.7082327622842399 1106 1 0.08854886243988247 1106 2 0.0433236176613363 1106 3 -0.001496019480068611 1106 4 -0.04456365745929247 1106 5 0.03846173637505501 1106 6 0.048748924581708764 1106 7 0.13289899023553886 1106 8 0.03761151528005513 1106 9 -0.049756883680451836 1106 10-11 -0.23433432420592482 1106 12-13 -0.3888313242300043 1106 14-15 -0.2995959238918644 1106 16-17 -0.20432374639471362 1106 18-19 0.029214056941931688 1106 20-21 0.07859844085402301 1106 22-23 0.15659998025249422 1106 24-25 0.11760020815808758 1106 26-27 0.07713683202512556 1106 28-29 0.22776930012510377 1106 30-31 0.08567116454012336 1106 32-33 0.21304350594085264 1106 34-35 0.19106388606406455 1106 36-37 0.19375439916961312 1106 38-39 0.1550383042620993 1106 40-41 0.16955420844050906 1106 42-43 0.04950011539543908 1106 44-45 0.12053508633763244 1106 46-47 -0.04045987115129179 1106 48-49 0.23372015627901277 1106 50-51 0.004785174945929782 1106 52-53 -0.09818956386906308 1106 54-55 -0.016488753978627813 1106 56-57 -0.06157440285780069 1106 58-59 -0.06975040807601118 1106 60-61 -0.23989035948929427 1106 62-63 -0.12060080582055122 1106 64-65 0.20559839677738267 1106 66-67 0.38200486982132276 1106 68-69 -0.019457620823580157 1106 70-71 0.13633655957738355 1106 72-73 -0.08186897482198319 1106 74-75 -0.06277603254293496 1106 76-77 0.4503042118235001 1106 78-79 0.33327139650879545 1106 80-81 0.08890078874353691 1106 82-83 0.10939714588950977 1106 84-85 -0.009335798609011903 1106 86-87 0.02632583303517677 1106 88-89 0.24859782933744512 1106 90-91 0.2132451372589408 1106 92-93 0.2446935319358836 1106 94-95 0.4886168704104037 1106 96-97 0.6913206572159609 1106 98-99 1.2208283491942957 1106 100-101 0.6642699660172156 1107 1 -0.032614272391636945 1107 2 -0.13926908975473395 1107 3 0.07507650254722265 1107 4 0.11142500454226223 1107 5 0.012459501080329005 1107 6 0.10572288782700667 1107 7 0.12884654157176811 1107 8 0.0813795239919699 1107 9 0.020949943607860178 1107 10-11 0.120445767602682 1107 12-13 0.13072913293726884 1107 14-15 0.15048542468385762 1107 16-17 0.1968693226284799 1107 18-19 0.12139488915935459 1107 20-21 -0.07562354371254543 1107 22-23 0.21541291630431658 1107 24-25 0.2740040800637189 1107 26-27 0.35930859801216997 1107 28-29 0.23242323325383296 1107 30-31 0.3391367832061434 1107 32-33 0.2974444443478035 1107 34-35 0.41755095626482586 1107 36-37 0.34527409998401026 1107 38-39 0.451015229663696 1107 40-41 0.5341075272539797 1107 42-43 0.45073975305885483 1107 44-45 0.3535118059253861 1107 46-47 0.42805354061709266 1107 48-49 0.48938655026403666 1107 50-51 0.38372722662344927 1107 52-53 0.34619184827446503 1107 54-55 0.5157185083845306 1107 56-57 0.5983738961819043 1107 58-59 0.6234599493106217 1107 60-61 0.7799309471941811 1107 62-63 0.6548279513243145 1107 64-65 0.7946881133795287 1107 66-67 1.0268906561604894 1107 68-69 0.9185377001678745 1107 70-71 0.9596333197026503 1107 72-73 0.6502304306625639 1107 74-75 0.6110757736689933 1107 76-77 0.7802003670123554 1107 78-79 0.8409034430279405 1107 80-81 1.0637605617751476 1107 82-83 0.9671540484692187 1107 84-85 0.9151155381389238 1107 86-87 0.9794012520878077 1107 88-89 1.110703200818417 1107 90-91 1.19177113232983 1107 92-93 1.2267102210944465 1107 94-95 1.0650718310452483 1107 96-97 1.0325795606189239 1107 98-99 1.334372138044916 1107 100-101 1.5608680454353134 1108 1 -0.1583411872677054 1108 2 -0.02013937005887456 1108 3 -0.23498553856045135 1108 4 -0.17478153464077195 1108 5 -0.1997159359884506 1108 6 -0.022165684700134136 1108 7 0.005193591568300349 1108 8 -0.08536185528665641 1108 9 -0.0502130527378668 1108 10-11 -0.03620726273472741 1108 12-13 0.10387674563934723 1108 14-15 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-2.0237280625155947 2316 68-69 -2.1332724198865733 2316 70-71 -2.478748652160874 2316 72-73 -2.3323685326457984 2316 74-75 -2.1046785546348694 2316 76-77 -2.299734605169565 2316 78-79 -2.466373849928477 2316 80-81 -2.550083033008832 2316 82-83 -2.5871539642764922 2316 84-85 -2.6892449668452336 2316 86-87 -2.807864427612497 2316 88-89 -2.8661224182812184 2316 90-91 -3.050696492109658 2316 92-93 -3.3103363065179146 2316 94-95 -3.3440620590506818 2316 96-97 -3.502553346199935 2316 98-99 -3.587859147008391 2316 100-101 -4.275033758388883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3990.0 3 818.0 4 725.0 5 856.0 6 962.0 7 1164.0 8 1486.0 9 1847.0 10 2358.0 11 3183.0 12 4200.0 13 5639.0 14 6314.0 15 8204.0 16 10942.0 17 14642.0 18 11979.0 19 7789.0 20 7566.0 21 8017.0 22 8587.0 23 9570.0 24 10851.0 25 12294.0 26 14124.0 27 17005.0 28 20305.0 29 24444.0 30 29832.0 31 37193.0 32 47293.0 33 61388.0 34 83024.0 35 117464.0 36 166888.0 37 222670.0 38 161528.0 39 17650.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.234971665617245 19.022409960058813 14.806319269361397 21.936299104962544 2 15.146004883475051 20.6519680266697 40.20506806902988 23.996959020825372 3 18.92441717812538 25.833565064521714 29.836502526372154 25.40551523098075 4 12.380270157877654 16.557480225473086 37.129273359857216 33.932976256792045 5 13.525199553324283 38.102007027307664 33.1320078280178 15.240785591350264 6 31.111554648597867 36.18075796732887 17.565116847274368 15.14257053679889 7 28.60133934907807 33.795037440699176 19.902710095870553 17.700913114352197 8 27.092615038663798 33.71479547200455 19.57620798016615 19.6163815091655 9 27.67561225481636 16.42170166023395 19.92272140395184 35.979964680997846 10-11 25.176681029439095 26.929983205192627 26.520523736431656 21.37281202893663 12-13 26.691079243516956 24.411874268203402 27.369214344030645 21.527832144248993 14-15 23.49307885996251 25.141661641373886 26.57746162640554 24.78779787225806 16-17 21.877134515098387 29.124807947407266 26.756861847730896 22.241195689763448 18-19 21.85911439079475 26.9407029011781 30.125835422561774 21.07434728546538 20-21 22.837573974676577 25.962315923479217 29.904090971648774 21.296019130195432 22-23 22.512261922580002 25.979577512038276 29.769204037359508 21.738956528022214 24-25 22.21833740379161 26.224158610685716 29.965582802324526 21.591921183198146 26-27 22.13448645115233 26.36195915348193 29.81735611472117 21.686198280644565 28-29 21.705122938920873 26.289638204939063 30.426140945875908 21.57909791026416 30-31 21.697715104006924 26.42567796890869 30.183881712099748 21.692725214984637 32-33 21.58403414299055 26.687330712044975 29.819198205789633 21.90943693917484 34-35 22.20295513440407 26.406244085085294 29.53620185904694 21.854598921463698 36-37 22.28115672765119 26.225281483584066 29.542271996146535 21.951289792618205 38-39 22.15925438666284 26.298559289996916 29.89313053265284 21.649055790687402 40-41 21.960983996996454 26.359347872552252 30.01469518774532 21.66497294270597 42-43 21.989001886349385 26.607934365707493 29.416814400595925 21.986249347347197 44-45 21.97154736421516 26.9245872251734 28.931892852519315 22.171972558092122 46-47 22.02387583362187 27.018058491706253 28.39981160844665 22.55825406622522 48-49 22.365376045478868 27.21458276338497 28.095013188390087 22.325028002746077 50-51 22.44834399225348 27.437338067508456 27.844107754391295 22.27021018584677 52-53 22.53881640826351 27.486138994710313 27.83684845960624 22.13819613741994 54-55 21.94604206535499 27.381838781733226 27.995437048431032 22.676682104480754 56-57 22.270248837692357 27.998981650354292 27.53736994862237 22.193399563330985 58-59 22.372358295581897 27.22988623393647 27.71855478308587 22.679200687395763 60-61 22.364962612176374 27.120707538974674 28.19013518790241 22.32419466094654 62-63 22.540029331188688 27.188205593569503 28.13652335098079 22.13524172426102 64-65 22.43796606087712 27.1893572552831 28.11464129976692 22.258035384072866 66-67 22.076913450758646 27.627244083043166 27.97282007815453 22.323022388043658 68-69 22.00877065485779 27.556437762149955 28.139370676702725 22.295420906289536 70-71 21.896498039748632 27.77621997478079 27.867351450104334 22.459930535366237 72-73 22.197106826871924 27.604743885325856 27.500540798110496 22.69760848969172 74-75 22.003646982423092 27.976483844469964 27.46573427776416 22.554134895342788 76-77 22.262414598964565 27.60525303994571 27.786957370658772 22.345374990430955 78-79 22.12439694241526 27.572278746999228 27.784644470190955 22.518679840394558 80-81 21.795345432587993 28.353870297790646 27.52850667718352 22.322277592437846 82-83 22.005236331609105 28.105029389857272 27.288271752536886 22.601462525996734 84-85 21.985305975011126 27.77850165667929 27.19452489534216 23.041667472967422 86-87 22.24348458037064 27.586633956081524 27.493963520960506 22.675917942587333 88-89 22.311231707998814 27.276421540591794 27.468984764370212 22.94336198703918 90-91 21.921987913983894 27.535164871080774 27.89008193238643 22.652765282548902 92-93 21.973676741509834 27.565025483831572 27.71274225981295 22.748555514845645 94-95 21.727602123617373 27.494274265889075 27.502229660635763 23.275893949857792 96-97 21.978403793419137 27.27936904778798 27.417102809244113 23.325124349548766 98-99 21.80280951182958 27.334662185553356 27.45438790013396 23.408140402483106 100-101 21.909549726665986 27.051660622916884 28.105264017908937 22.933525632508193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2061.0 1 1210.0 2 422.5 3 522.0 4 483.0 5 510.5 6 951.5 7 1874.0 8 2898.0 9 3301.0 10 2829.5 11 2350.5 12 2355.0 13 2474.0 14 2579.5 15 2584.0 16 2571.5 17 2663.5 18 2725.0 19 2803.5 20 2958.0 21 3240.5 22 3720.5 23 4288.0 24 4964.0 25 5700.0 26 6812.0 27 8093.0 28 9039.0 29 10268.5 30 11789.5 31 13322.5 32 15362.5 33 17662.5 34 20501.0 35 22640.0 36 25065.0 37 27897.5 38 29510.5 39 30043.0 40 30380.0 41 32611.0 42 35067.5 43 37162.5 44 39286.5 45 41426.0 46 42030.0 47 43154.0 48 51891.0 49 53093.0 50 46236.5 51 42994.5 52 40053.0 53 39718.5 54 38807.0 55 35756.5 56 32438.0 57 28844.5 58 25625.0 59 22968.5 60 20309.5 61 17026.0 62 13767.0 63 10614.5 64 7722.5 65 5989.0 66 4609.5 67 3603.5 68 2588.0 69 1692.0 70 1276.5 71 978.0 72 801.0 73 664.0 74 507.5 75 382.0 76 259.0 77 149.0 78 83.0 79 51.5 80 30.0 81 17.0 82 12.5 83 11.5 84 10.5 85 7.0 86 6.5 87 9.0 88 8.5 89 5.0 90 5.5 91 5.5 92 4.0 93 3.5 94 3.5 95 3.0 96 3.0 97 3.5 98 4.5 99 4.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32932890221696043 2 0.2854584462647011 3 0.2786761252857804 4 0.285372594100411 5 0.285372594100411 6 0.20784808974641847 7 0.19642975189583042 8 0.20055065578175693 9 0.19282396099564472 10-11 0.21712012348975313 12-13 0.2072471245963875 14-15 0.20089406443891747 16-17 0.20183843824610895 18-19 0.19595756499223466 20-21 0.19170788285987295 22-23 0.1932532218170954 24-25 0.18436752281306637 26-27 0.18441044889521144 28-29 0.1996492080567105 30-31 0.2096939112786564 32-33 0.20497204224269894 34-35 0.21265581094666608 36-37 0.1891323179311689 38-39 0.18943280050618436 40-41 0.1860845660988691 42-43 0.19145032636700254 44-45 0.19441222603501224 46-47 0.19921994723525982 48-49 0.20664615944635656 50-51 0.21158265889303934 52-53 0.23351788686916905 54-55 0.20497204224269894 56-57 0.18265047952726365 58-59 0.1840670402380509 60-61 0.18157732747363695 62-63 0.18956157875261959 64-65 0.18226414478795802 66-67 0.17998906243426946 68-69 0.1761686411233584 70-71 0.1877586833025267 72-73 0.18539774878454798 74-75 0.18784453546681687 76-77 0.18801623979539714 78-79 0.18681430949533523 80-81 0.19011961782050543 82-83 0.18432459673092128 84-85 0.17818616698417658 86-87 0.1777569061627259 88-89 0.17668375410909923 90-91 0.18260755344511856 92-93 0.17986028418783423 94-95 0.1768554584376795 96-97 0.17535304556260212 98-99 0.18333729684158473 100-101 0.17355015011250927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1164793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.27935861147344 #Duplication Level Percentage of deduplicated Percentage of total 1 73.73285573877536 36.33507839399149 2 13.758121487798853 13.559828052349163 3 5.102773414251302 7.543842029819478 4 2.4126977855885676 4.755847975885075 5 1.289081009365762 3.176254266988777 6 0.7840104569554285 2.318131947807103 7 0.5199664908889339 1.7936530619325646 8 0.36505033043644913 1.4391556915851722 9 0.2818167847129589 1.2498975356942077 >10 1.5468188594798886 14.010066863144763 >50 0.13661517916762256 4.712732501519551 >100 0.06583809322477475 5.725610852055569 >500 0.0024384468382846504 0.8100844128033422 >1k 0.001567572967468704 1.3945159413875716 >5k 3.4834954832637863E-4 1.175300473036254 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7877 0.6762574981133986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5816 0.49931618751142903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4153 0.3565440382969335 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2355 0.20218184690326949 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2118 0.18183488396650735 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1627 0.13968147130005074 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1487 0.12766216829943175 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 0.10963321379850324 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.2926082145067836E-5 0.0 22-23 0.0 0.0 0.0 1.287782464352035E-4 0.0 24-25 0.0 0.0 0.0 4.2926082145067836E-4 0.0 26-27 0.0 0.0 0.0 6.438912321760175E-4 0.0 28-29 0.0 0.0 0.0 0.0017170432858027134 0.0 30-31 0.0 0.0 0.0 0.004421386460941987 0.0 32-33 0.0 0.0 0.0 0.007383286128951668 0.0 34-35 0.0 0.0 0.0 0.009872998893365603 0.0 36-37 0.0 0.0 0.0 0.011332485686297907 0.0 38-39 0.0 0.0 0.0 0.012148081247054198 0.0 40-41 0.0 0.0 0.0 0.015024128750773743 0.0 42-43 0.0 0.0 0.0 0.017986028418783426 0.0 44-45 0.0 0.0 0.0 0.021505967154678986 0.0 46-47 0.0 0.0 0.0 0.02597027969776604 0.0 48-49 0.0 0.0 0.0 0.0329672310874121 0.0 50-51 0.0 0.0 0.0 0.04138074318784539 0.0 52-53 0.0 0.0 0.0 0.06288671034252438 0.0 54-55 0.0 0.0 0.0 0.08636727727587648 0.0 56-57 0.0 0.0 0.0 0.11688772168101971 0.0 58-59 0.0 0.0 0.0 0.16754049861219977 0.0 60-61 0.0 0.0 0.0 0.24347673792682478 0.0 62-63 0.0 0.0 0.0 0.29584655814380756 0.0 64-65 0.0 0.0 0.0 0.3517792431788309 0.0 66-67 0.0 0.0 0.0 0.45849348339146956 0.0 68-69 0.0 0.0 0.0 0.5889887731124758 0.0 70-71 0.0 0.0 0.0 0.7061769773685109 0.0 72-73 0.0 0.0 0.0 0.8204891341208266 0.0 74-75 0.0 0.0 0.0 1.0290669672637112 0.0 76-77 0.0 0.0 0.0 1.1808965198108163 0.0 78-79 0.0 0.0 0.0 1.3075284621387664 0.0 80-81 0.0 0.0 0.0 1.4627921012574765 0.0 82-83 0.0 0.0 0.0 1.7255426500674367 0.0 84-85 0.0 0.0 0.0 2.0082109009927085 0.0 86-87 0.0 0.0 0.0 2.3573716531606905 0.0 88-89 0.0 0.0 0.0 2.782468644643297 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7390 0.0 27.743423 1 GTATCAA 10170 0.0 27.549997 1 TACATGG 7630 0.0 26.482996 2 ACATGGG 7770 0.0 25.210352 3 ATAGCGT 95 0.0012195392 25.02464 4 GGACCGT 115 1.2818084E-4 24.782486 6 GGTATCA 7320 0.0 24.499516 1 GAGTACT 5625 0.0 23.221605 12-13 AGTACTT 5775 0.0 22.947445 12-13 TATCAAC 12330 0.0 22.519176 2 ATCAACG 12420 0.0 22.31771 3 GTATAGG 320 0.0 22.298132 1 TCAACGC 12400 0.0 22.277983 4 CATGGGG 2780 0.0 22.234121 4 GTATAGC 300 7.2759576E-12 22.19903 1 CAACGCA 12550 0.0 21.896168 5 AACGCAG 12725 0.0 21.687515 6 GTACTTT 6185 0.0 21.155209 14-15 CATGGGA 5175 0.0 21.131916 4 ATGGGAG 1715 0.0 20.791275 5 >>END_MODULE