FastQCFastQC Report
Thu 26 May 2016
SRR937278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164793
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111900.9606857184066182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85420.7333491873663389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64070.5500548166068993No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25110.21557478453253068No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24060.20656030728206645No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.16887120715869688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.13487375009980315No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12430.10671424021263863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81050.028.6757051
GTATCAA113950.028.4881731
TATCAAC139750.023.2848342
ATCAACG141850.022.906633
GAGTACT77200.022.88878812-13
TCAACGC142750.022.7622094
CAACGCA144100.022.5819285
AACGCAG146500.022.2415476
GTACATG78800.022.1359461
AGTACTT79100.021.94866212-13
GTACTTT81300.021.82119814-15
GTATAGG3351.8189894E-1221.2816221
TACATGG81250.020.9311962
ACGCAGA160600.020.2601287
ACATGGG81800.020.2097243
CGCAGAG161600.020.1641488
TACTTTT88100.019.651714-15
ACTTTTT91750.019.54375616-17
GCAGAGT165450.019.4078359
CATATAG2803.484638E-818.662473
Produced by FastQC (version 0.11.5)