##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937278_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1164793 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.135869635205566 33.0 31.0 34.0 31.0 34.0 2 32.347824033969985 34.0 31.0 34.0 31.0 34.0 3 32.42290432720664 34.0 31.0 34.0 31.0 34.0 4 36.06312452083761 37.0 35.0 37.0 35.0 37.0 5 35.9226154346738 37.0 35.0 37.0 35.0 37.0 6 36.021548893236826 37.0 35.0 37.0 35.0 37.0 7 35.98290683408983 37.0 35.0 37.0 35.0 37.0 8 35.96144465153894 37.0 35.0 37.0 35.0 37.0 9 37.64729269492519 39.0 37.0 39.0 35.0 39.0 10-11 37.571498970203294 39.0 37.0 39.0 35.0 39.0 12-13 37.47582789388329 39.0 37.0 39.0 35.0 39.0 14-15 38.63739007703515 40.0 38.0 41.0 34.0 41.0 16-17 38.630230435794175 40.0 38.0 41.0 34.5 41.0 18-19 38.680795643517776 40.0 38.0 41.0 34.0 41.0 20-21 38.62081932154469 40.0 38.0 41.0 34.0 41.0 22-23 38.49634570262699 40.0 38.0 41.0 34.0 41.0 24-25 38.34311246719374 40.0 38.0 41.0 34.0 41.0 26-27 38.11553039896359 40.0 37.5 41.0 33.5 41.0 28-29 38.07325550548467 40.0 37.0 41.0 33.0 41.0 30-31 38.1282678553185 40.0 37.0 41.0 34.0 41.0 32-33 38.0321546403524 40.0 37.0 41.0 33.5 41.0 34-35 37.89009763966645 40.0 37.0 41.0 33.0 41.0 36-37 37.70814170414829 40.0 37.0 41.0 33.0 41.0 38-39 37.548916846169234 40.0 36.0 41.0 33.0 41.0 40-41 37.348258016660466 40.0 36.0 41.0 33.0 41.0 42-43 37.094068645673524 40.0 36.0 41.0 32.0 41.0 44-45 36.71541638728941 39.5 35.5 41.0 31.0 41.0 46-47 36.24834927751111 39.0 35.0 41.0 30.0 41.0 48-49 35.83239983413362 39.0 35.0 41.0 28.5 41.0 50-51 35.580535769016464 39.0 35.0 41.0 28.0 41.0 52-53 35.32044749582115 39.0 35.0 40.0 27.0 41.0 54-55 35.094655445216446 38.5 35.0 40.0 27.0 41.0 56-57 34.86117747960367 38.0 34.5 40.0 26.0 41.0 58-59 34.594726273251986 38.0 34.0 40.0 26.0 41.0 60-61 34.25345233015652 37.5 34.0 40.0 25.5 41.0 62-63 33.86903638672279 37.0 33.5 40.0 24.5 41.0 64-65 33.50662521151827 36.5 33.0 39.0 23.0 41.0 66-67 33.11942036052758 36.0 33.0 39.0 22.5 40.5 68-69 32.694803282643356 35.5 32.5 39.0 22.0 40.0 70-71 32.31583122494727 35.0 32.0 38.0 20.5 40.0 72-73 31.882992514549798 35.0 32.0 37.0 19.5 39.5 74-75 31.45423822086843 35.0 31.0 37.0 18.0 39.0 76-77 30.14162215947383 33.5 29.5 35.5 17.0 37.5 78-79 30.755002390982774 34.0 31.0 36.0 16.0 37.5 80-81 30.60716496407516 34.0 31.0 35.5 13.0 37.0 82-83 30.299909082558017 34.0 31.0 35.0 8.5 37.0 84-85 29.98960759551268 34.0 30.5 35.0 7.0 36.0 86-87 29.660956925393613 34.0 30.0 35.0 2.0 36.0 88-89 29.301684505315535 34.0 30.0 35.0 2.0 35.5 90-91 28.866150466220176 34.0 29.0 35.0 2.0 35.0 92-93 28.481988215931928 34.0 29.0 35.0 2.0 35.0 94-95 28.104047242728967 34.0 29.0 35.0 2.0 35.0 96-97 27.68989082180267 33.5 28.0 35.0 2.0 35.0 98-99 27.066492930503532 33.0 27.0 35.0 2.0 35.0 100-101 25.50160672325469 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12916914109842992 1101 2 0.22155689351596664 1101 3 0.22678691283988428 1101 4 0.11354865865111918 1101 5 -0.03323197101876474 1101 6 0.10669798292466481 1101 7 0.10422268362916043 1101 8 0.10797328657346839 1101 9 0.07531059157405906 1101 10-11 0.16696685628843255 1101 12-13 0.17475710694333912 1101 14-15 0.2507105853969236 1101 16-17 0.20765892259581165 1101 18-19 0.24396253167815019 1101 20-21 0.17175850308264984 1101 22-23 0.2542232777846607 1101 24-25 0.2687844291556658 1101 26-27 0.15747124075925 1101 28-29 0.21018764789119615 1101 30-31 0.22606674301599838 1101 32-33 0.14154376468827934 1101 34-35 0.004041005779676254 1101 36-37 0.2114986845832192 1101 38-39 0.2991824743820004 1101 40-41 0.015299304999551566 1101 42-43 -0.025728180073564033 1101 44-45 0.2630866444830602 1101 46-47 0.20345405381366533 1101 48-49 0.23199834546775122 1101 50-51 0.23657652076401803 1101 52-53 0.3081234543477862 1101 54-55 0.23604028446762726 1101 56-57 0.07325627021816672 1101 58-59 0.21890695413554795 1101 60-61 0.34473329545017606 1101 62-63 0.07993694459508305 1101 64-65 0.31369931022909014 1101 66-67 0.2851701323301512 1101 68-69 0.10678126631736973 1101 70-71 0.1362791096318361 1101 72-73 0.05638460480423291 1101 74-75 -0.012764452174568675 1101 76-77 0.07449821200813389 1101 78-79 0.13916051704908128 1101 80-81 0.023643042752496513 1101 82-83 0.208357028892582 1101 84-85 0.12093577081465767 1101 86-87 0.07117261732095415 1101 88-89 0.1486878992242282 1101 90-91 0.3851910950186834 1101 92-93 0.47832227929353976 1101 94-95 0.475401928076149 1101 96-97 0.4291497766751142 1101 98-99 0.29239819407455414 1101 100-101 0.4505405062286947 1102 1 0.18582993493750877 1102 2 0.16770916353171827 1102 3 0.10579227376523193 1102 4 0.09753303980790662 1102 5 0.06704810186734278 1102 6 0.11559082814859067 1102 7 0.16364994245775932 1102 8 0.21290502266216293 1102 9 0.2693277084337353 1102 10-11 0.23772380688610184 1102 12-13 0.1621062002870346 1102 14-15 0.29515733694204727 1102 16-17 0.2964482635165382 1102 18-19 0.20480063968197726 1102 20-21 0.11214039448947943 1102 22-23 0.28730470313431056 1102 24-25 0.3389328533944749 1102 26-27 0.26912177942156745 1102 28-29 0.25483312989135243 1102 30-31 0.401678678630077 1102 32-33 0.307963731479731 1102 34-35 0.5042541270607543 1102 36-37 0.36591533189079684 1102 38-39 0.30734173011588695 1102 40-41 0.4737985611308986 1102 42-43 0.5841964873784349 1102 44-45 0.5212265411295149 1102 46-47 0.5877833351015553 1102 48-49 0.5441679132564232 1102 50-51 0.4374947239939999 1102 52-53 0.5556836953735669 1102 54-55 0.8616608888412074 1102 56-57 0.7408782136238514 1102 58-59 0.6968889902729671 1102 60-61 0.39173099223272345 1102 62-63 0.3782111419124945 1102 64-65 0.4331260345412886 1102 66-67 0.4627827354044456 1102 68-69 0.4954284806988838 1102 70-71 0.30261512993796913 1102 72-73 0.4047124936083719 1102 74-75 0.18669034110121174 1102 76-77 0.2205424823007398 1102 78-79 0.09094405435524422 1102 80-81 0.09371540119645161 1102 82-83 0.12358032996990787 1102 84-85 0.14557560612878717 1102 86-87 0.14029723707763964 1102 88-89 0.19179047908817637 1102 90-91 0.03243220234477917 1102 92-93 0.1177519091820507 1102 94-95 -0.05615167124076592 1102 96-97 0.06087483240530389 1102 98-99 -0.12507487280921126 1102 100-101 0.18347761215134284 1103 1 0.22690000855890702 1103 2 0.23093469655197651 1103 3 0.20453585667303997 1103 4 0.18913186131875648 1103 5 0.184419196071282 1103 6 0.15680308607639404 1103 7 0.17924031493962644 1103 8 0.2011912232650772 1103 9 0.19998004729721686 1103 10-11 0.23251869918832568 1103 12-13 0.25083775221506954 1103 14-15 0.4138132167416231 1103 16-17 0.2944518728104697 1103 18-19 0.2218384894177774 1103 20-21 0.20203334611263557 1103 22-23 0.2697134966867907 1103 24-25 0.16730853440945026 1103 26-27 0.27666814312629384 1103 28-29 0.23763771533523226 1103 30-31 0.16943223494668302 1103 32-33 0.2738186488009262 1103 34-35 0.2423769524269872 1103 36-37 0.293965287799999 1103 38-39 0.20831206964928128 1103 40-41 0.23636409920963075 1103 42-43 0.2514192450704016 1103 44-45 0.15162937387838582 1103 46-47 0.3949674022562917 1103 48-49 0.337675627438621 1103 50-51 0.36848987742317263 1103 52-53 0.4053965924471683 1103 54-55 0.38485265308868577 1103 56-57 0.4341921333307255 1103 58-59 0.26276680747043457 1103 60-61 0.2651715790164175 1103 62-63 0.438342115533203 1103 64-65 0.38029465282428276 1103 66-67 0.2069473684253822 1103 68-69 0.38658434340899106 1103 70-71 0.3343372689914901 1103 72-73 0.3367090893879663 1103 74-75 0.175839901218513 1103 76-77 -0.09091394983017764 1103 78-79 0.4063584054000948 1103 80-81 0.3732928130319273 1103 82-83 0.5348094722840955 1103 84-85 0.40786497853546066 1103 86-87 0.4012201362703358 1103 88-89 0.6368420550781657 1103 90-91 0.7184735314946131 1103 92-93 0.7916327204528919 1103 94-95 0.8169536203396 1103 96-97 0.8474418333418043 1103 98-99 0.732408601683062 1103 100-101 1.166806160435904 1104 1 0.10428190175043284 1104 2 0.15034479875270534 1104 3 0.21194574456031035 1104 4 0.1330795152560924 1104 5 -0.10274476712037739 1104 6 0.08939212662182427 1104 7 0.14469432461052634 1104 8 0.03677663387627206 1104 9 0.13013261169171386 1104 10-11 0.14537817653137353 1104 12-13 0.1196665291549408 1104 14-15 0.1495660597436128 1104 16-17 0.18267936654849848 1104 18-19 0.21849185079045697 1104 20-21 0.09168803354985045 1104 22-23 -0.03168556837073311 1104 24-25 0.033717578437475026 1104 26-27 0.17899009552191814 1104 28-29 0.1509747098568468 1104 30-31 0.20276869368464645 1104 32-33 0.13747041621724065 1104 34-35 0.19770264191357256 1104 36-37 0.2176261635383696 1104 38-39 0.22253846503082286 1104 40-41 0.38514987951154467 1104 42-43 0.4427770547868377 1104 44-45 0.5250236972506102 1104 46-47 0.42961318229031775 1104 48-49 0.46240807047811927 1104 50-51 0.5656174073265916 1104 52-53 0.7820330766348533 1104 54-55 0.7198868762693635 1104 56-57 0.5700889681888128 1104 58-59 0.7198257570665945 1104 60-61 0.5336092338913758 1104 62-63 0.6780182343164398 1104 64-65 0.7085317906680118 1104 66-67 0.40766130013975044 1104 68-69 0.24053775702057578 1104 70-71 0.49434503785442274 1104 72-73 0.5399612961468705 1104 74-75 0.3765397686120586 1104 76-77 0.46198731863761466 1104 78-79 0.6162958526588902 1104 80-81 0.5639072229332065 1104 82-83 0.5621391265696936 1104 84-85 0.4399463640719823 1104 86-87 0.5437961840622343 1104 88-89 0.4578441044506292 1104 90-91 0.5443853682949111 1104 92-93 0.4207458645652977 1104 94-95 0.5309107844367347 1104 96-97 0.4155359169508408 1104 98-99 0.636979505951917 1104 100-101 1.0503241158854948 1105 1 0.012260571145333188 1105 2 0.09804265888018193 1105 3 0.0018638332887377373 1105 4 0.028062658731279555 1105 5 -0.02415788757609505 1105 6 0.009385648780792621 1105 7 -0.06636890245965787 1105 8 0.0844099726506542 1105 9 0.12630245965524267 1105 10-11 0.12709305641497082 1105 12-13 0.011027094269451254 1105 14-15 1.6938043749803455E-5 1105 16-17 0.03386864160868441 1105 18-19 -0.02357247262861506 1105 20-21 0.09831368245857419 1105 22-23 -0.04563638304921369 1105 24-25 0.010144470731802357 1105 26-27 0.03424248069126179 1105 28-29 -0.1396438596970384 1105 30-31 -0.17837384837955028 1105 32-33 -0.04366878410355923 1105 34-35 -0.052694190113697914 1105 36-37 -0.054859270958964146 1105 38-39 -0.1993563163227634 1105 40-41 -0.16052050634413462 1105 42-43 -0.2309058806005524 1105 44-45 -0.05732521417647973 1105 46-47 -0.2004628937831896 1105 48-49 -0.3844558961455746 1105 50-51 -0.26891681148304514 1105 52-53 -0.26990275675095887 1105 54-55 -0.3272956484691747 1105 56-57 -0.3905160544514956 1105 58-59 -0.27017503981068103 1105 60-61 -0.3113762873627479 1105 62-63 -0.3454677952682701 1105 64-65 -0.32512181449471456 1105 66-67 -0.4156163845976053 1105 68-69 -0.5223931832817428 1105 70-71 -0.403604545520146 1105 72-73 -0.35561597074403295 1105 74-75 -0.24884469253824548 1105 76-77 -0.18932168080585754 1105 78-79 -0.17600997598421841 1105 80-81 -0.153698752103967 1105 82-83 -0.27488308445625975 1105 84-85 -0.38396657323048444 1105 86-87 -0.5097671426176014 1105 88-89 -0.47106030497976903 1105 90-91 -0.4067268538744706 1105 92-93 -0.29177969655840386 1105 94-95 -0.2549740770801314 1105 96-97 -0.30645609628246007 1105 98-99 -0.3468352098006733 1105 100-101 -0.11824145361827121 1106 1 -0.11193065205080188 1106 2 -0.06283959056969479 1106 3 -0.05271744090235586 1106 4 0.03723951826145111 1106 5 -0.0761795669958758 1106 6 -0.06972939709365988 1106 7 -0.11107220216398161 1106 8 -0.0626134398622824 1106 9 -0.07677273032852838 1106 10-11 -0.06169822927754609 1106 12-13 -0.18380868202498135 1106 14-15 -0.012022807559183946 1106 16-17 -0.021916278905365516 1106 18-19 -5.932329838458372E-4 1106 20-21 -0.26970602228536933 1106 22-23 -0.16692927053995987 1106 24-25 -0.019373913185098957 1106 26-27 0.05874835220078012 1106 28-29 -0.06651914526353409 1106 30-31 -0.14383605907115538 1106 32-33 -0.11644392577079543 1106 34-35 0.02235957516246856 1106 36-37 -0.04752874646194982 1106 38-39 -0.03344809673460958 1106 40-41 -0.040857317242675606 1106 42-43 -0.39745875832296207 1106 44-45 -0.05020344862722226 1106 46-47 0.04043836717337257 1106 48-49 -0.18898136998900128 1106 50-51 0.13865024084061162 1106 52-53 0.11060703522533544 1106 54-55 0.2142585422025789 1106 56-57 0.12257536358632848 1106 58-59 -0.0011343199993305575 1106 60-61 0.1844767620934249 1106 62-63 0.3113580971434047 1106 64-65 0.032816408612141856 1106 66-67 0.11846220067320701 1106 68-69 0.1479130884147395 1106 70-71 0.19923451052178365 1106 72-73 -0.002750983952303443 1106 74-75 -0.1770273474268791 1106 76-77 -0.09786622392573818 1106 78-79 -0.059015684688024095 1106 80-81 0.018973126096344828 1106 82-83 -0.25977005264612174 1106 84-85 -0.1796480202057147 1106 86-87 -0.026272885458087814 1106 88-89 -0.27427939421306036 1106 90-91 -0.08645025483344781 1106 92-93 -0.10484393654453683 1106 94-95 -0.2730038258866614 1106 96-97 -0.11068648475791676 1106 98-99 -0.20583232656388617 1106 100-101 -0.05147896353103931 1107 1 -0.1154470728697845 1107 2 -0.09572160973382893 1107 3 -0.06671473666467165 1107 4 0.010785274067224293 1107 5 -0.16255104275933974 1107 6 -0.13323404223410762 1107 7 -0.06956033009161189 1107 8 -0.08038312113738755 1107 9 -0.06777814401436899 1107 10-11 -0.09743526430759175 1107 12-13 -0.10410857104181304 1107 14-15 -0.05215650840484187 1107 16-17 0.0314842739262744 1107 18-19 -0.10308332005091358 1107 20-21 0.0014159497760459772 1107 22-23 -0.05037610543556781 1107 24-25 -0.08101711533597467 1107 26-27 0.033974812567294066 1107 28-29 -0.0948914963871701 1107 30-31 -0.1401216401175276 1107 32-33 0.08682753748752248 1107 34-35 0.03177132530966276 1107 36-37 -0.04139568911022451 1107 38-39 0.21394666465158707 1107 40-41 0.04119955772150519 1107 42-43 -0.0015224095127663873 1107 44-45 0.06907902957996015 1107 46-47 -0.14711145801511094 1107 48-49 -0.07939721817653123 1107 50-51 0.08285775330667633 1107 52-53 0.07217585781379654 1107 54-55 -0.15773631505638974 1107 56-57 -0.14707595127759987 1107 58-59 -0.04374527711188847 1107 60-61 -0.24751934568008238 1107 62-63 -0.20084028135607923 1107 64-65 -0.2882825070875512 1107 66-67 -0.11020643121179319 1107 68-69 -0.2881963654751374 1107 70-71 -0.24947387730446025 1107 72-73 -0.1752366681661215 1107 74-75 -0.015512648670622298 1107 76-77 -0.1700645503204541 1107 78-79 -0.029524383579758506 1107 80-81 0.059803076961546964 1107 82-83 -0.046828594963013614 1107 84-85 -0.18373303435216215 1107 86-87 -0.019076565097105203 1107 88-89 0.04094014924492484 1107 90-91 0.07241687018153087 1107 92-93 -6.285302054713782E-4 1107 94-95 -0.16641211627698027 1107 96-97 0.14998294433009463 1107 98-99 0.0754839441933548 1107 100-101 0.26672520641388786 1108 1 -0.11860155994982335 1108 2 -0.055038906122540254 1108 3 -0.05905541636342804 1108 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2316 50-51 -0.9972075471983501 2316 52-53 -0.8278984092798609 2316 54-55 -0.6247243513173402 2316 56-57 -0.5587590633735928 2316 58-59 -0.6567014362680297 2316 60-61 -0.6936809159077981 2316 62-63 -0.3898143448167488 2316 64-65 -0.5193494108314596 2316 66-67 -0.5760948974359081 2316 68-69 -0.7156616359846097 2316 70-71 -0.7422408558768794 2316 72-73 -0.6357776630404075 2316 74-75 -0.46323896079612936 2316 76-77 -0.41798912190118287 2316 78-79 -0.3902090203166253 2316 80-81 -0.7684827406702333 2316 82-83 -0.6051615473366319 2316 84-85 -0.5260778694607673 2316 86-87 -0.5961038121378515 2316 88-89 -0.658187894121923 2316 90-91 -0.47172874144327537 2316 92-93 -0.8615938740998068 2316 94-95 -1.0355480435048534 2316 96-97 -0.9417798244369813 2316 98-99 -0.8284805230926331 2316 100-101 -0.7825569451824137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 7.0 5 19.0 6 58.0 7 133.0 8 296.0 9 598.0 10 1138.0 11 1917.0 12 2900.0 13 4279.0 14 5379.0 15 7710.0 16 11313.0 17 16155.0 18 13491.0 19 7112.0 20 5600.0 21 5635.0 22 5959.0 23 6369.0 24 7226.0 25 8667.0 26 10437.0 27 12958.0 28 16311.0 29 20279.0 30 25469.0 31 32604.0 32 41543.0 33 55166.0 34 76122.0 35 112230.0 36 170718.0 37 258439.0 38 202953.0 39 17598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.989967747338405 19.400040369509856 15.528518481934645 21.0814734012171 2 15.970305453415328 21.345852868277884 39.16386860154551 23.51997307676128 3 19.207790568796344 25.828881183180187 30.581141885296358 24.38218636272711 4 12.956390497187481 17.291241698088587 36.89808995940906 32.85427784531487 5 13.496751836161344 38.040866932610264 33.243235353210984 15.21914587801741 6 31.137292205567856 36.84920840011917 16.828140279002362 15.185359115310618 7 28.25334630273362 34.25475599527126 19.802574362998403 17.689323338996715 8 27.376967409659912 33.82824244307787 19.12391300428488 19.670877142977336 9 27.612202339814885 16.2768835320954 19.846702375443535 36.26421175264618 10-11 25.202203309944345 27.177618684178217 26.207446301617544 21.412731704259897 12-13 26.94450991073832 24.48057325291926 27.42617011877947 21.148746717562954 14-15 23.5702824450353 25.06947586395179 26.921349973772163 24.438891717240747 16-17 21.60821708234854 28.957892088980618 27.236341564552674 22.197549264118173 18-19 21.598945048605202 26.258957600191625 31.310241390530337 20.83185596067284 20-21 22.50777605978058 25.244657205185817 31.415453217867896 20.832113517165713 22-23 22.242483637404806 25.18240639505209 31.305946939989727 21.26916302755338 24-25 22.024794275338365 25.491313847619946 31.445545744493618 21.038346132548067 26-27 21.951238358196655 25.622699112612985 31.341563298442267 21.084499230748097 28-29 21.506852490515204 25.499523935852825 31.895095895374066 21.098527678257906 30-31 21.47901622708475 25.722002676898057 31.621309070117043 21.177672025900147 32-33 21.52914723903732 25.75891166928373 31.27851901582513 21.433422075853823 34-35 22.065165226782785 25.402024222329633 31.10801661754492 21.424793933342663 36-37 22.14990592728907 25.3100450252642 31.07468682136401 21.465362226082714 38-39 22.0398381337041 25.39752925101853 31.350670100194154 21.21196251508321 40-41 21.873080398440063 25.51411517502044 31.49771311199205 21.115091314547442 42-43 21.805937047343715 25.826662533819082 30.85803239248085 21.50936802635635 44-45 21.988198761496676 25.958432099094004 30.260484051672698 21.792885087736618 46-47 21.99686983008998 26.258742969780897 29.596031226149194 22.14835597397993 48-49 22.42103207223604 26.363322222627634 29.208550020711844 22.00709568442448 50-51 22.508868536185002 26.64385572703109 28.841844724208272 22.005431012575634 52-53 22.588291552608343 26.84137032249978 28.719946874612322 21.850391250279557 54-55 22.02219621855557 26.790167866736837 28.795502720225823 22.392133194481765 56-57 22.30636688235592 27.274416999415347 28.38040750588302 22.03880861234571 58-59 22.496314795847848 26.501747520804127 28.614225875327204 22.387711808020825 60-61 22.416257652647296 26.533684525920055 29.05889716026796 21.991160661164688 62-63 22.604145114196257 26.495609091057382 29.02412703373046 21.876118761015906 64-65 22.45433308750997 26.48204444909954 29.025887003098404 22.037735460292083 66-67 22.15397929074093 26.99260727013298 28.83340645075992 22.020006988366173 68-69 22.15526707320528 26.867649445008684 28.907196385967293 22.069887095818743 70-71 22.06310477483982 27.135164788936745 28.6729916817838 22.12873875443963 72-73 22.40428127572882 26.989087331397084 28.234287122261208 22.37234427061289 74-75 22.210598793090274 27.377310818317074 28.038072000776104 22.374018387816548 76-77 22.429564738112266 26.983421088553932 28.58190253547197 22.005111637861834 78-79 22.183855843913896 26.897568924263798 28.596282772990566 22.32229245883174 80-81 21.811557933469725 27.76883961356224 28.210119738013535 22.209482714954504 82-83 22.034815762689966 27.517699565200033 28.017575722974602 22.429908949135402 84-85 21.987187412001454 27.157896002032984 27.898071071916704 22.956845514048858 86-87 22.256229218410482 26.94598954492343 28.103062089143737 22.69471914752235 88-89 22.284560432626225 26.62910920652854 28.266567536034298 22.819762824810933 90-91 21.92098263424418 26.953797782444667 28.575108224736546 22.55011135857461 92-93 21.84667214130226 26.843686561801494 28.41486118879723 22.89478010809901 94-95 21.636505370482137 26.80042720036951 28.307690722729273 23.25537670641908 96-97 21.945315605433755 26.67718641853102 28.186166984176587 23.19133099185864 98-99 21.81779859826584 26.617066094079394 28.089329108756523 23.475806198898237 100-101 21.36210801797609 26.85771273627108 28.59796609820222 23.182213147550613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 890.0 1 628.5 2 497.5 3 650.0 4 523.5 5 449.5 6 952.0 7 2310.5 8 3768.5 9 4294.0 10 3571.0 11 2695.5 12 2589.5 13 2702.0 14 2784.0 15 2847.5 16 2906.5 17 3000.0 18 3059.0 19 3137.5 20 3358.5 21 3615.0 22 3964.0 23 4459.0 24 5078.5 25 5803.0 26 6913.0 27 8177.5 28 9095.5 29 10183.5 30 11737.5 31 13207.0 32 15124.5 33 17400.0 34 19995.0 35 21954.5 36 24533.0 37 27635.0 38 29660.0 39 30130.0 40 30158.5 41 32529.0 42 35325.5 43 37580.0 44 39656.5 45 41635.5 46 41983.5 47 42873.5 48 52263.0 49 53172.5 50 45820.5 51 43314.5 52 40036.5 53 39431.0 54 38482.5 55 35255.5 56 31934.0 57 28433.5 58 25421.5 59 22827.0 60 20120.0 61 16748.5 62 13441.0 63 10455.0 64 7733.5 65 5922.0 66 4385.0 67 3360.0 68 2437.5 69 1530.0 70 1062.5 71 846.5 72 736.5 73 628.5 74 471.0 75 361.0 76 258.0 77 145.0 78 73.5 79 47.0 80 29.5 81 14.5 82 5.5 83 4.5 84 2.5 85 1.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04704698603099435 2 0.0 3 0.0 4 8.585216429013567E-5 5 0.011590042179168316 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006138429746744701 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015024128750773741 24-25 6.868173143210854E-4 26-27 0.002146304107253392 28-29 0.002918973585864613 30-31 0.001030225971481628 32-33 0.0 34-35 0.0 36-37 2.1463041072533918E-4 38-39 3.863347393056105E-4 40-41 4.2926082145067836E-5 42-43 0.012019303000618993 44-45 0.0 46-47 0.0 48-49 4.2926082145067836E-5 50-51 8.585216429013567E-5 52-53 1.287782464352035E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.863347393056105E-4 84-85 8.585216429013567E-5 86-87 0.0 88-89 0.0 90-91 0.007469138293241804 92-93 0.002360934517978731 94-95 0.0 96-97 0.0 98-99 0.001845821532237917 100-101 0.011590042179168316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1164793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.77298976296562 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99360546868328 33.67356171585709 2 14.608764044522667 13.665911422080782 3 5.346262694007344 7.501820707707917 4 2.529109985596129 4.731761418628076 5 1.4647070821826194 3.425436468033545 6 0.8736007961036737 2.4516552655845447 7 0.5931716217193092 1.9421087133251276 8 0.3909597294075748 1.4629084337049847 9 0.30664496313584166 1.2908431549455925 >10 1.6631338225558365 14.359212421149694 >50 0.15159865172680656 4.913201201313591 >100 0.07236471542527909 6.012302343585459 >500 0.004235084044571665 1.3101337821630858 >1k 0.0012889386222609417 1.0079210920756372 >5k 3.6826817778884044E-4 1.2874829022847734 >10k+ 1.8413408889442022E-4 0.9637389575601454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11190 0.9606857184066182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8542 0.7333491873663389 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6407 0.5500548166068993 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2511 0.21557478453253068 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2406 0.20656030728206645 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.16887120715869688 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.13487375009980315 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1243 0.10671424021263863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.2926082145067836E-5 0.0 22-23 0.0 0.0 0.0 1.287782464352035E-4 0.0 24-25 0.0 0.0 0.0 4.2926082145067836E-4 0.0 26-27 0.0 0.0 0.0 6.438912321760175E-4 0.0 28-29 0.0 0.0 0.0 0.0017170432858027134 0.0 30-31 0.0 0.0 0.0 0.00437846037879692 0.0 32-33 0.0 0.0 0.0 0.007254507882516465 0.0 34-35 0.0 0.0 0.0 0.009787146729075467 0.0 36-37 0.0 0.0 0.0 0.011332485686297907 0.0 38-39 0.0 0.0 0.0 0.012148081247054198 0.0 40-41 0.0 0.0 0.0 0.014981202668628676 0.0 42-43 0.0 0.0 0.0 0.017900176254493288 0.0 44-45 0.0 0.0 0.0 0.021334262826098713 0.0 46-47 0.0 0.0 0.0 0.025884427533475904 0.0 48-49 0.0 0.0 0.0 0.033010157169557165 0.0 50-51 0.0 0.0 0.0 0.04176707792715101 0.0 52-53 0.0 0.0 0.0 0.06310134075324972 0.0 54-55 0.0 0.0 0.0 0.08679653809732717 0.0 56-57 0.0 0.0 0.0 0.11761746507748586 0.0 58-59 0.0 0.0 0.0 0.16857072458368139 0.0 60-61 0.0 0.0 0.0 0.24472159430903173 0.0 62-63 0.0 0.0 0.0 0.29674800586885397 0.0 64-65 0.0 0.0 0.0 0.35238020832886185 0.0 66-67 0.0 0.0 0.0 0.4602534527594173 0.0 68-69 0.0 0.0 0.0 0.5920794510269207 0.0 70-71 0.0 0.0 0.0 0.7098686204329868 0.0 72-73 0.0 0.0 0.0 0.8246529640888982 0.0 74-75 0.0 0.0 0.0 1.0359780664890672 0.0 76-77 0.0 0.0 0.0 1.1892671058291044 0.0 78-79 0.0 0.0 0.0 1.3172297567035516 0.0 80-81 0.0 0.0 0.0 1.475111886833111 0.0 82-83 0.0 0.0 0.0 1.742756009007609 0.0 84-85 0.0 0.0 0.0 2.027527637957989 0.0 86-87 0.0 0.0 0.0 2.379993698451141 0.0 88-89 0.0 0.0 0.0 2.810714006694752 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8105 0.0 28.675705 1 GTATCAA 11395 0.0 28.488173 1 TATCAAC 13975 0.0 23.284834 2 ATCAACG 14185 0.0 22.90663 3 GAGTACT 7720 0.0 22.888788 12-13 TCAACGC 14275 0.0 22.762209 4 CAACGCA 14410 0.0 22.581928 5 AACGCAG 14650 0.0 22.241547 6 GTACATG 7880 0.0 22.135946 1 AGTACTT 7910 0.0 21.948662 12-13 GTACTTT 8130 0.0 21.821198 14-15 GTATAGG 335 1.8189894E-12 21.281622 1 TACATGG 8125 0.0 20.931196 2 ACGCAGA 16060 0.0 20.260128 7 ACATGGG 8180 0.0 20.209724 3 CGCAGAG 16160 0.0 20.164148 8 TACTTTT 8810 0.0 19.6517 14-15 ACTTTTT 9175 0.0 19.543756 16-17 GCAGAGT 16545 0.0 19.407835 9 CATATAG 280 3.484638E-8 18.66247 3 >>END_MODULE