FastQCFastQC Report
Thu 26 May 2016
SRR937277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227174
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80440.6554897675472263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58560.4771939431572051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.3575695052209385No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25780.21007615871913846No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21180.1725916618181285No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18460.1504269158244878No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15120.12320991155288492No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.11017182567427275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12360.10071921341227894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105550.027.703011
GTACATG80800.027.5976181
TACATGG82500.026.2113822
GGTATCA75550.025.6135651
ACATGGG82600.025.142943
TCAACGC127050.023.0055664
ATCAACG127250.022.9684683
CATGGGG27950.022.9553764
TATCAAC127150.022.9500942
GAGTACT61300.022.9382112-13
GTGTAGG4600.022.739191
CAACGCA130350.022.424985
ATGGGAG18700.022.3670675
AGTACTT61850.022.23512-13
AACGCAG132600.022.0694796
TATAGGG1302.9307377E-421.9351372
GTACTTT64300.021.64547714-15
CATGGGA57350.021.3805434
GTATAGG3600.021.1313651
ACGCAGA145100.020.0381057
Produced by FastQC (version 0.11.5)