##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937277_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1227174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.580903767517892 31.0 31.0 34.0 30.0 34.0 2 31.65232232755909 33.0 31.0 34.0 30.0 34.0 3 31.69095091649595 33.0 31.0 34.0 30.0 34.0 4 35.348514554578244 37.0 35.0 37.0 33.0 37.0 5 35.33036635391558 37.0 35.0 37.0 33.0 37.0 6 35.41697998816794 37.0 35.0 37.0 33.0 37.0 7 35.35411359758274 37.0 35.0 37.0 33.0 37.0 8 35.341283306197816 37.0 35.0 37.0 33.0 37.0 9 36.92719532845383 39.0 37.0 39.0 33.0 39.0 10-11 36.834558098525555 39.0 37.0 39.0 33.0 39.0 12-13 36.70962878939743 39.0 37.0 39.0 32.0 39.0 14-15 37.800804531386746 40.0 37.5 41.0 32.5 41.0 16-17 37.756506819733794 40.0 37.0 41.0 32.5 41.0 18-19 37.73810600615724 40.0 37.5 41.0 32.0 41.0 20-21 37.68715235166325 40.0 37.0 41.0 32.0 41.0 22-23 37.54592176822521 40.0 37.0 41.0 32.0 41.0 24-25 37.39237182339261 40.0 37.0 41.0 32.0 41.0 26-27 37.21727970116707 39.0 36.5 41.0 31.5 41.0 28-29 37.02581581747984 39.0 36.0 41.0 31.0 41.0 30-31 36.9894623745288 39.0 36.0 41.0 31.0 41.0 32-33 36.82895864808087 39.0 36.0 41.0 31.0 41.0 34-35 36.614659779297796 39.0 35.0 40.5 30.0 41.0 36-37 36.58933207515805 39.0 35.0 41.0 30.0 41.0 38-39 36.55081390251097 39.0 35.0 41.0 30.0 41.0 40-41 36.36200897346261 39.0 35.0 41.0 30.0 41.0 42-43 36.09166059580793 39.0 35.0 41.0 30.0 41.0 44-45 35.82067905610777 39.0 35.0 40.5 28.5 41.0 46-47 35.422759934614 39.0 35.0 40.0 26.5 41.0 48-49 35.06522383948813 38.5 35.0 40.0 25.5 41.0 50-51 34.099530302956225 37.5 33.5 39.5 23.5 40.5 52-53 34.20906652194391 38.0 33.5 39.5 24.0 40.5 54-55 34.546466923191005 38.0 34.0 40.0 24.0 41.0 56-57 34.32624346669665 38.0 34.0 40.0 23.0 41.0 58-59 34.067864051878544 38.0 34.0 40.0 23.0 41.0 60-61 33.76738873216023 37.5 33.0 40.0 22.0 41.0 62-63 33.431289287419716 37.0 33.0 40.0 21.5 41.0 64-65 33.04561170624541 36.0 33.0 39.0 20.0 41.0 66-67 32.649661743159484 36.0 32.0 39.0 18.5 40.5 68-69 32.156196268825774 35.0 31.5 39.0 17.0 40.0 70-71 31.732109708973624 35.0 31.0 38.0 13.5 40.0 72-73 31.313794946763863 35.0 31.0 37.0 9.5 39.5 74-75 30.86100463340977 35.0 30.5 37.0 7.5 39.0 76-77 30.42992884464632 34.5 30.0 36.0 6.0 39.0 78-79 29.967464679010476 34.0 30.0 36.0 2.0 37.5 80-81 29.54214724236335 34.0 29.0 35.0 2.0 37.0 82-83 29.12591246229141 34.0 29.0 35.0 2.0 37.0 84-85 28.67723199807036 34.0 29.0 35.0 2.0 36.0 86-87 28.227244465739986 34.0 27.5 35.0 2.0 36.0 88-89 27.846379160575438 33.5 27.0 35.0 2.0 35.5 90-91 27.415063389543782 33.0 26.0 35.0 2.0 35.0 92-93 26.979869195403424 33.0 25.5 35.0 2.0 35.0 94-95 26.43264769299219 33.0 25.0 35.0 2.0 35.0 96-97 25.777870945766452 32.0 23.5 35.0 2.0 35.0 98-99 25.039242601293704 32.0 19.0 34.0 2.0 35.0 100-101 23.206777930432033 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19878805514063558 1101 2 0.2522075317000656 1101 3 0.32537113812439244 1101 4 0.24307432194115108 1101 5 0.28585987925075074 1101 6 0.11448881770461838 1101 7 0.15582772494263963 1101 8 0.2484805652383386 1101 9 0.3234954272260353 1101 10-11 0.358539170315062 1101 12-13 0.518345094175622 1101 14-15 0.6154569714155613 1101 16-17 0.546111572971391 1101 18-19 0.571737832260304 1101 20-21 0.4665635938967938 1101 22-23 0.4474491549327766 1101 24-25 0.6034828921243829 1101 26-27 0.6740026395947396 1101 28-29 0.6000567924395526 1101 30-31 0.32196893308054797 1101 32-33 0.5483316080224299 1101 34-35 0.30472920151634497 1101 36-37 0.3658585937015886 1101 38-39 0.369964708625524 1101 40-41 0.552685468489365 1101 42-43 0.6148192815878559 1101 44-45 0.48508169425227976 1101 46-47 0.4073911479461856 1101 48-49 0.4756214873168574 1101 50-51 0.5034838531946448 1101 52-53 0.4426158754576477 1101 54-55 0.5049315387710394 1101 56-57 0.6217048632098425 1101 58-59 0.6316800957078925 1101 60-61 0.5558506770330212 1101 62-63 0.5820010139140877 1101 64-65 0.4094742225346977 1101 66-67 0.6192404715290607 1101 68-69 0.5755306056606102 1101 70-71 0.5701997743200344 1101 72-73 0.43616856044893737 1101 74-75 0.4990674006184257 1101 76-77 0.6672001472383293 1101 78-79 0.8292647141716039 1101 80-81 0.8362439765757799 1101 82-83 0.8602973207490017 1101 84-85 0.5963344053686193 1101 86-87 0.8595276552509539 1101 88-89 0.6752271349065389 1101 90-91 0.5202453172895076 1101 92-93 0.5155998523222038 1101 94-95 0.6655392878022575 1101 96-97 0.5848290880686946 1101 98-99 0.6529619577532451 1101 100-101 0.9447221177349867 1102 1 0.13634609004450837 1102 2 0.062138405681231745 1102 3 0.15318361894266985 1102 4 -0.028079114835250607 1102 5 0.1044461979670146 1102 6 0.07366750709201852 1102 7 0.13439643892181863 1102 8 0.1729460564274774 1102 9 0.2961937188613035 1102 10-11 0.15253370866739857 1102 12-13 0.2667054244864602 1102 14-15 0.3170658197549301 1102 16-17 0.2904710047615211 1102 18-19 0.3682509711666384 1102 20-21 0.4403909133666133 1102 22-23 0.638215026295434 1102 24-25 0.6171996987713513 1102 26-27 0.4697560191688055 1102 28-29 0.4651219678404601 1102 30-31 0.5117533534106258 1102 32-33 0.4087402782248404 1102 34-35 0.3575793274790229 1102 36-37 0.33951809353376916 1102 38-39 0.5303326972581957 1102 40-41 0.42242935090853706 1102 42-43 0.325922672850524 1102 44-45 0.32966111995649783 1102 46-47 0.1901225072330135 1102 48-49 0.12594870296052108 1102 50-51 0.2116549717615115 1102 52-53 0.1722414613571459 1102 54-55 0.1572794465107208 1102 56-57 0.1324998450761754 1102 58-59 0.158846307672583 1102 60-61 0.17446977608317837 1102 62-63 0.25736633179844404 1102 64-65 0.3212621481403204 1102 66-67 0.3263429642710278 1102 68-69 0.46974938239258535 1102 70-71 0.3848400460703125 1102 72-73 0.19337958090986973 1102 74-75 0.08741660389959449 1102 76-77 0.12719312101536673 1102 78-79 0.2966877881310914 1102 80-81 0.4342950485971748 1102 82-83 0.37395829634920474 1102 84-85 0.31353774661189604 1102 86-87 0.18087483555454398 1102 88-89 0.313813505862381 1102 90-91 0.420277052831878 1102 92-93 0.14566007149334226 1102 94-95 0.34740647390444224 1102 96-97 0.5280276911824338 1102 98-99 0.45293466257482606 1102 100-101 0.5914699429361434 1103 1 0.12729602643527116 1103 2 0.013516733956144833 1103 3 0.14225154182239663 1103 4 0.1202559630011919 1103 5 -0.04630385295880757 1103 6 0.10252611522195565 1103 7 0.17821442810119947 1103 8 0.20606257599644806 1103 9 0.09967063953359911 1103 10-11 0.2566296183307486 1103 12-13 0.19677497827374424 1103 14-15 0.32753027609131635 1103 16-17 0.27943739627927044 1103 18-19 0.2909279528218178 1103 20-21 0.3065117235164223 1103 22-23 0.3143610495329838 1103 24-25 0.14213221404589405 1103 26-27 0.10956022058140746 1103 28-29 0.01998157270730161 1103 30-31 0.19651737146463688 1103 32-33 0.02550070392630488 1103 34-35 0.17793479614394414 1103 36-37 0.11862940905992758 1103 38-39 0.00862964798633925 1103 40-41 0.015081362894996175 1103 42-43 0.055597823738310126 1103 44-45 -0.02749674497668053 1103 46-47 -0.061490464183187044 1103 48-49 -0.16654521476640838 1103 50-51 -0.19753371258421737 1103 52-53 -0.3304620914077816 1103 54-55 -0.20022272824621012 1103 56-57 -0.07439768960142601 1103 58-59 0.147519635479874 1103 60-61 0.12592689525730094 1103 62-63 0.2585100809897156 1103 64-65 0.09555985218633367 1103 66-67 0.13789435067055678 1103 68-69 0.15075481832405302 1103 70-71 0.09552434823276812 1103 72-73 0.14767349274349684 1103 74-75 0.2681552397536784 1103 76-77 0.4011372268795874 1103 78-79 0.5429285654321347 1103 80-81 0.4369252553624321 1103 82-83 0.47038682659786346 1103 84-85 0.6539153920323493 1103 86-87 0.7979943043373332 1103 88-89 0.6274044875713614 1103 90-91 0.9472325084290425 1103 92-93 0.8121705442771763 1103 94-95 0.8955131221908381 1103 96-97 0.9535453089556114 1103 98-99 1.1921143660391884 1103 100-101 1.3419888098333352 1104 1 0.10487154985762004 1104 2 0.09131890147098431 1104 3 0.10316466404778879 1104 4 -0.030341776956092303 1104 5 0.08122958996428764 1104 6 0.09492209518081296 1104 7 0.08713598611323192 1104 8 0.19087914439325715 1104 9 0.24089500980262102 1104 10-11 0.16633249555412277 1104 12-13 0.37503264873667064 1104 14-15 0.4826873520134001 1104 16-17 0.32124289578217 1104 18-19 0.2717862473557844 1104 20-21 0.2707408383002132 1104 22-23 0.26082714889218295 1104 24-25 0.40830859778067463 1104 26-27 0.6453950878432764 1104 28-29 0.5439379271087788 1104 30-31 0.3144639661433004 1104 32-33 0.3155860707402951 1104 34-35 0.4058314602185149 1104 36-37 0.45332748762988473 1104 38-39 0.5250422731204551 1104 40-41 0.5040370774760206 1104 42-43 0.5447534822119877 1104 44-45 0.6401768186263936 1104 46-47 0.7001438095221602 1104 48-49 0.5837439348629871 1104 50-51 0.34751826052363555 1104 52-53 0.5067909625044749 1104 54-55 0.7846535157124066 1104 56-57 0.9387315492090949 1104 58-59 0.7615479450742342 1104 60-61 0.7114475122410866 1104 62-63 0.8123232878682742 1104 64-65 0.6555276396871861 1104 66-67 0.8154445273594391 1104 68-69 0.8314068938031838 1104 70-71 0.9300593956286995 1104 72-73 0.9334499148675093 1104 74-75 0.7545812026834646 1104 76-77 0.5794164297617321 1104 78-79 0.7033973815674237 1104 80-81 0.7450492525669112 1104 82-83 0.9418723666555486 1104 84-85 0.9425870017221847 1104 86-87 0.906637048281187 1104 88-89 0.881308622780189 1104 90-91 1.097137519699725 1104 92-93 1.2984224022173443 1104 94-95 1.152740017289812 1104 96-97 1.091169458732658 1104 98-99 1.0886670872448114 1104 100-101 1.3202244557074785 1105 1 0.14189887905016718 1105 2 0.15731641699893473 1105 3 0.10853236591114168 1105 4 0.17587561434825716 1105 5 0.28578362723136763 1105 6 0.1896500454913692 1105 7 0.15512853269707705 1105 8 0.19986796426903197 1105 9 0.36290867439889496 1105 10-11 0.34449522847338443 1105 12-13 0.3018164996683552 1105 14-15 0.3233115756158824 1105 16-17 0.3290944485772016 1105 18-19 0.3465850051197563 1105 20-21 0.32816940972878683 1105 22-23 0.38486627932696393 1105 24-25 0.35779306983036463 1105 26-27 0.4690721064768084 1105 28-29 0.33763482151871216 1105 30-31 0.3146409847768368 1105 32-33 0.32440656763470344 1105 34-35 0.5003233856843607 1105 36-37 0.47425730129447885 1105 38-39 0.33676277001486454 1105 40-41 0.3459029159853273 1105 42-43 0.4167242896654031 1105 44-45 0.4567320981294998 1105 46-47 0.2618997101432825 1105 48-49 0.1976557361052258 1105 50-51 0.1910101859894766 1105 52-53 0.320966117783982 1105 54-55 0.5509696647807303 1105 56-57 0.47351229455071575 1105 58-59 0.7222758953847901 1105 60-61 0.6712251519926369 1105 62-63 0.6727027909738652 1105 64-65 0.5862102483828409 1105 66-67 0.7918569497196941 1105 68-69 0.8833037882131194 1105 70-71 0.7857836192311858 1105 72-73 0.8729362502712306 1105 74-75 0.7443029418139027 1105 76-77 0.7186915850412383 1105 78-79 0.6913222262879195 1105 80-81 0.6462678861072817 1105 82-83 0.8848705033015101 1105 84-85 0.7954012874364693 1105 86-87 0.8354656197097547 1105 88-89 0.8350862625317426 1105 90-91 1.0281884513767459 1105 92-93 0.969908737621072 1105 94-95 0.9526300794016116 1105 96-97 0.781757036372408 1105 98-99 0.8811260934559932 1105 100-101 1.1004623439062335 1106 1 0.17976960663053987 1106 2 0.24504298214275977 1106 3 0.18089243359303708 1106 4 0.2114830593567163 1106 5 0.21150410100463546 1106 6 0.1696717036978015 1106 7 0.23993259361078145 1106 8 0.2108946986175937 1106 9 0.18454116170853752 1106 10-11 -0.007290219919177332 1106 12-13 -0.005413957184437379 1106 14-15 0.23207976512518513 1106 16-17 0.02814757886485353 1106 18-19 0.2883690998744086 1106 20-21 0.09809157554942516 1106 22-23 0.26575155851477916 1106 24-25 0.34941405121784186 1106 26-27 0.4666660151062487 1106 28-29 0.46155834097725545 1106 30-31 0.3124480913758205 1106 32-33 0.4178150278715975 1106 34-35 0.40966484084510313 1106 36-37 0.04677303733509319 1106 38-39 0.09036344683381259 1106 40-41 0.3677431867129144 1106 42-43 0.32721701386168434 1106 44-45 0.39473142131055283 1106 46-47 0.3446726373852371 1106 48-49 0.5365071743454948 1106 50-51 0.5199218611163801 1106 52-53 0.4097255086469289 1106 54-55 0.4012464837316614 1106 56-57 0.47770315749487224 1106 58-59 0.32487623379426367 1106 60-61 0.365903324581474 1106 62-63 0.5650012681312191 1106 64-65 0.6306021265554307 1106 66-67 0.7054535656249428 1106 68-69 0.614298035252034 1106 70-71 0.5315034161854975 1106 72-73 0.8799264363964454 1106 74-75 0.7948592869915672 1106 76-77 0.9050455613525727 1106 78-79 0.6929120740036581 1106 80-81 0.7750712702020373 1106 82-83 0.5568481682761295 1106 84-85 0.6691853821911202 1106 86-87 0.6250622356150011 1106 88-89 0.8090300865588169 1106 90-91 0.9885180622058449 1106 92-93 0.8705334415127801 1106 94-95 1.0194341403153189 1106 96-97 1.1781062749510873 1106 98-99 1.362952827804552 1106 100-101 1.401587893821631 1107 1 0.2147710407920016 1107 2 0.09788749009380382 1107 3 0.1518144809966806 1107 4 0.13888027997811747 1107 5 0.13626325398097805 1107 6 0.19987461780863214 1107 7 0.05746383596301996 1107 8 0.1691158647355948 1107 9 0.19213169150620502 1107 10-11 -0.020714102786335786 1107 12-13 0.16636766607582132 1107 14-15 0.23930846519597537 1107 16-17 0.2019547907233985 1107 18-19 0.21266774228927687 1107 20-21 0.27490657924666806 1107 22-23 0.24300562613878895 1107 24-25 0.39551313203876504 1107 26-27 0.2217066321377743 1107 28-29 0.3530817888593063 1107 30-31 0.37123818539892284 1107 32-33 0.20142243077623334 1107 34-35 0.25688388335154144 1107 36-37 0.18661562166773393 1107 38-39 0.3135808104503255 1107 40-41 0.44622142298377554 1107 42-43 0.30379676244923104 1107 44-45 0.41140519299730727 1107 46-47 0.2725099745289725 1107 48-49 0.5056924390601196 1107 50-51 0.4530419122725249 1107 52-53 0.26473967921477026 1107 54-55 0.49497090894869444 1107 56-57 0.3274102727777688 1107 58-59 0.3894398145138709 1107 60-61 0.3745694754763065 1107 62-63 0.41987728553062453 1107 64-65 0.559303249938047 1107 66-67 0.4276249123946556 1107 68-69 0.37441545228777073 1107 70-71 0.4601058587335167 1107 72-73 0.673601258047281 1107 74-75 0.7037156945355179 1107 76-77 0.4494374637348564 1107 78-79 0.627699831264593 1107 80-81 0.6469901256096158 1107 82-83 0.8065383104554762 1107 84-85 0.8707001987895033 1107 86-87 0.7640298498808313 1107 88-89 0.9410091241180574 1107 90-91 0.9286556364467309 1107 92-93 1.0580138698139194 1107 94-95 0.898396175824633 1107 96-97 0.7280170674766104 1107 98-99 0.982138224093628 1107 100-101 1.0086421261768415 1108 1 -0.037170180533465214 1108 2 -0.18576527718627744 1108 3 -0.19912707414628628 1108 4 -0.027848721617282024 1108 5 0.03485641976546816 1108 6 -0.1497796745373492 1108 7 -0.09605481260084048 1108 8 -0.12581301204109252 1108 9 -0.13903647994425228 1108 10-11 -0.16087419004779946 1108 12-13 -0.06232104519884274 1108 14-15 0.09968343491237164 1108 16-17 -0.048472529168670064 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-2.3206723223267964 2316 94-95 -2.209904064820801 2316 96-97 -2.3985849271375805 2316 98-99 -2.8311713869957913 2316 100-101 -3.1218684988392056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4493.0 3 793.0 4 782.0 5 850.0 6 1089.0 7 1246.0 8 1596.0 9 2023.0 10 2673.0 11 3439.0 12 4484.0 13 6002.0 14 7008.0 15 8788.0 16 11390.0 17 14902.0 18 12683.0 19 8270.0 20 8144.0 21 8639.0 22 9270.0 23 10106.0 24 11745.0 25 13471.0 26 15438.0 27 18048.0 28 21857.0 29 26376.0 30 32220.0 31 40250.0 32 50744.0 33 65330.0 34 87631.0 35 124153.0 36 175391.0 37 229622.0 38 167436.0 39 18792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.201205394516535 19.021835103599834 14.794860558811218 21.982098943072415 2 15.209527888016424 20.697641728396675 40.0871847846696 24.0056455989173 3 19.01776162498478 25.751781842491745 29.73848330286296 25.491973229660513 4 12.410691372594531 16.60049396599775 37.045243166286085 33.94357149512164 5 13.692467978182263 38.04400318686033 33.10093767236624 15.162591162591163 6 31.2690168793944 36.179393583053646 17.496263994708347 15.055325542843612 7 28.82892521082384 33.60670062932734 19.859989775268236 17.70438438458058 8 27.191929387860437 33.73219261538662 19.48995991807215 19.58591807868079 9 27.713984959213157 16.373698793911494 19.798095116766582 36.11422113010877 10-11 25.164523115722346 26.904817517771274 26.51717345285386 21.41348591365252 12-13 26.694167595319833 24.332400800853613 27.3524258162994 21.62100578752715 14-15 23.54591107663414 25.087412730167802 26.525129628873557 24.841546564324503 16-17 21.9243364638628 29.093221723152308 26.703103307472436 22.279338505512456 18-19 21.92196483412036 26.93607903626578 29.99992650979502 21.14202961981884 20-21 22.93035058457983 25.961223994006193 29.734463772790654 21.373961648623325 22-23 22.574717055086477 25.940332511309634 29.67312309124463 21.811827342359262 24-25 22.3045484551958 26.214416267362438 29.775841212932274 21.70519406450949 26-27 22.214332757479532 26.376118413289362 29.74104544617509 21.668503383056013 28-29 21.77786742635671 26.304118013824844 30.20592723193997 21.71208732787847 30-31 21.757401453809237 26.465110334247083 30.052958968539457 21.724529243404223 32-33 21.698715723707046 26.68052351103579 29.603446515711457 22.017314249545706 34-35 22.26097061246955 26.383489021017226 29.402107030485062 21.953433336028162 36-37 22.355007610548938 26.200539597718798 29.431429933954806 22.013022857777457 38-39 22.237522203229926 26.30781157699168 29.724424798053178 21.730241421725218 40-41 21.999681566366878 26.364345231048098 29.86384920942727 21.77212399315776 42-43 22.021054316151144 26.640903312302967 29.272325405372747 22.065716966173145 44-45 22.033771655459535 26.98665924061549 28.755273503533633 22.22429560039134 46-47 22.09946894315728 27.009182747328076 28.260822523636662 22.63052578587798 48-49 22.45816935765087 27.177239100023602 27.981635267994587 22.382956274330944 50-51 22.50549226183184 27.359467516027603 27.79260075952468 22.342439462615868 52-53 22.623158226604847 27.43754847040768 27.7807567325626 22.15853657042487 54-55 22.037633185679347 27.324304755541924 27.871850986404045 22.766211072374684 56-57 22.364579961210293 27.968267718367635 27.41761065700421 22.24954166341786 58-59 22.46702076898681 27.145032696572297 27.705207165367796 22.682739369073097 60-61 22.44214089308924 27.08348300883684 28.115631674166767 22.35874442390715 62-63 22.585518704754627 27.18261597960238 28.042109680458573 22.18975563518442 64-65 22.424429452726084 27.158454353564803 28.04905875874613 22.368057434962978 66-67 22.125116760619502 27.60147559294798 27.833853720989477 22.439553925443036 68-69 22.030243135410775 27.59457511105233 27.992575556812827 22.38260619672407 70-71 22.00096341473371 27.746793380197744 27.70882830806411 22.543414897004432 72-73 22.261534736798268 27.53630703064811 27.464613633422186 22.737544599131432 74-75 22.044457798322064 27.947928586329212 27.371906535013036 22.635707080335685 76-77 22.35166299616128 27.62069593435389 27.67507984063719 22.35256122884764 78-79 22.120861749105067 27.562237072456952 27.72946552219696 22.587435656241016 80-81 21.85350361372993 28.264572792012032 27.487085074203417 22.39483852005462 82-83 22.07253238701849 28.074750924833047 27.17863965336265 22.674077034785807 84-85 21.988788486257874 27.770069674990193 27.098627930490736 23.142513908261193 86-87 22.391176737126823 27.56534865816551 27.33630076425498 22.70717384045269 88-89 22.36986068720677 27.24184838078068 27.36240595855726 23.025884973455288 90-91 21.974636312978017 27.58322396017252 27.760071686197346 22.682068040652116 92-93 22.073743536774806 27.502015770983924 27.610775546197385 22.813465146043885 94-95 21.845927360553393 27.436447424501974 27.438162082542007 23.27946313240263 96-97 22.093837349193475 27.269461405408848 27.329961597482672 23.30673964791501 98-99 21.880182412763993 27.321578025549222 27.392126202850502 23.40611335883628 100-101 21.97894645751636 27.1063236958686 27.992174211553444 22.922555635061588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2274.0 1 1356.5 2 499.0 3 552.5 4 524.5 5 551.0 6 969.0 7 2003.5 8 3049.0 9 3368.0 10 2921.5 11 2466.5 12 2510.5 13 2641.0 14 2675.0 15 2671.0 16 2621.5 17 2695.5 18 2753.0 19 2862.0 20 3065.5 21 3337.0 22 3821.5 23 4434.0 24 5073.5 25 5743.0 26 6916.5 27 8238.0 28 9166.0 29 10507.5 30 12220.5 31 13891.0 32 16005.5 33 18281.5 34 21140.0 35 23552.5 36 26068.5 37 29046.0 38 30864.0 39 31302.5 40 31672.0 41 34215.0 42 36819.5 43 39065.0 44 41406.5 45 43692.5 46 44420.0 47 45365.0 48 54728.0 49 56091.0 50 48814.0 51 45716.5 52 42788.0 53 42182.0 54 41217.5 55 38203.0 56 34681.0 57 30853.5 58 27150.0 59 24452.0 60 21759.0 61 18249.0 62 14880.5 63 11425.5 64 8321.5 65 6544.5 66 4981.0 67 3813.5 68 2766.0 69 1819.5 70 1390.5 71 1070.0 72 855.0 73 690.0 74 507.5 75 372.0 76 264.5 77 157.0 78 94.0 79 61.5 80 34.0 81 22.5 82 15.0 83 8.5 84 5.5 85 4.5 86 3.5 87 4.0 88 4.5 89 3.5 90 3.0 91 4.0 92 3.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.326441075185752 2 0.29058633901956854 3 0.2918086595706884 4 0.2940088365627042 5 0.2769778368837671 6 0.21219484767441293 7 0.21936579490764962 8 0.21855091454023634 9 0.2148839528868767 10-11 0.22213638815685469 12-13 0.22250308432219065 14-15 0.20567580473510685 16-17 0.21789901024630576 18-19 0.20543134062488289 20-21 0.20995392666402646 22-23 0.2081204458373466 24-25 0.1987900656304648 26-27 0.21313196009693813 28-29 0.21508767297873 30-31 0.2229105245058973 32-33 0.2240106130019052 34-35 0.2124393117846369 36-37 0.20844639798431194 38-39 0.19626393649148371 40-41 0.19793444124468085 42-43 0.19858634553861146 44-45 0.21643222558496186 46-47 0.20702035734133872 48-49 0.2161877614747379 50-51 0.22197341208337204 52-53 0.23179272051070182 54-55 0.22837022296756612 56-57 0.19267846287486534 58-59 0.17915144877580522 60-61 0.18758546057853245 62-63 0.18945968542358296 64-65 0.18542602760488733 66-67 0.1993197378692834 68-69 0.18750397254179113 70-71 0.1926377188564947 72-73 0.20490166838606425 74-75 0.1951231039771051 76-77 0.20755002958015734 78-79 0.20388306792679767 80-81 0.18998935766240158 82-83 0.2031904196144964 84-85 0.18518156349466333 86-87 0.18913373327661767 88-89 0.19948271394276607 90-91 0.19487863986688114 92-93 0.20017536225506735 94-95 0.19883080964883545 96-97 0.19430822360969185 98-99 0.20200884308174716 100-101 0.18330733864961285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1227174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.623073929860055 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26397438810928 35.62319643068411 2 13.957648148963072 13.573275156680115 3 5.1658244730830045 7.535347957901858 4 2.388082348205076 4.644636182694768 5 1.3619021602744836 3.3109934712131173 6 0.8393669897995452 2.448756191958443 7 0.5716161636834057 1.9455614490396858 8 0.37731875417470534 1.4677118143487513 9 0.275456983817496 1.2054208759788936 >10 1.5875451945692332 14.26470489789169 >50 0.13729523465965557 4.660863177070373 >100 0.06994363158156731 6.044012186974533 >500 0.0020127645397753687 0.6635368973616919 >1k 0.0016773037831461406 1.4783595548765227 >5k 3.354607566292282E-4 1.1336237553254076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8044 0.6554897675472263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5856 0.4771939431572051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4388 0.3575695052209385 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2578 0.21007615871913846 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2118 0.1725916618181285 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1846 0.1504269158244878 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1512 0.12320991155288492 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1352 0.11017182567427275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1236 0.10071921341227894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.0744018370663004E-5 0.0 24-25 0.0 0.0 0.0 8.148803674132601E-5 0.0 26-27 0.0 0.0 0.0 1.22232055111989E-4 0.0 28-29 0.0 0.0 0.0 0.0012223205511198902 0.0 30-31 0.0 0.0 0.0 0.003707705671730333 0.0 32-33 0.0 0.0 0.0 0.00611160275559945 0.0 34-35 0.0 0.0 0.0 0.00867847591295122 0.0 36-37 0.0 0.0 0.0 0.011082372996820336 0.0 38-39 0.0 0.0 0.0 0.012793621768388183 0.0 40-41 0.0 0.0 0.0 0.015564215017593266 0.0 42-43 0.0 0.0 0.0 0.019149688634211612 0.0 44-45 0.0 0.0 0.0 0.022327722067123325 0.0 46-47 0.0 0.0 0.0 0.02701328417974957 0.0 48-49 0.0 0.0 0.0 0.03283967880675438 0.0 50-51 0.0 0.0 0.0 0.04188485088504157 0.0 52-53 0.0 0.0 0.0 0.06449778108075954 0.0 54-55 0.0 0.0 0.0 0.08857749593782137 0.0 56-57 0.0 0.0 0.0 0.12247651922221299 0.0 58-59 0.0 0.0 0.0 0.17426216657132565 0.0 60-61 0.0 0.0 0.0 0.24870148813452697 0.0 62-63 0.0 0.0 0.0 0.30313549667773276 0.0 64-65 0.0 0.0 0.0 0.35744727316582653 0.0 66-67 0.0 0.0 0.0 0.4645225534439289 0.0 68-69 0.0 0.0 0.0 0.6013409671326153 0.0 70-71 0.0 0.0 0.0 0.7251212949426895 0.0 72-73 0.0 0.0 0.0 0.8390415703070633 0.0 74-75 0.0 0.0 0.0 1.0466730879239619 0.0 76-77 0.0 0.0 0.0 1.2024782141733774 0.0 78-79 0.0 0.0 0.0 1.3291921113061391 0.0 80-81 0.0 0.0 0.0 1.4841823571881412 0.0 82-83 0.0 8.148803674132601E-5 0.0 1.751177909571096 0.0 84-85 0.0 8.148803674132601E-5 0.0 2.0205773590379197 0.0 86-87 0.0 8.148803674132601E-5 0.0 2.3618492569105927 0.0 88-89 0.0 8.148803674132601E-5 0.0 2.775441787391193 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10555 0.0 27.70301 1 GTACATG 8080 0.0 27.597618 1 TACATGG 8250 0.0 26.211382 2 GGTATCA 7555 0.0 25.613565 1 ACATGGG 8260 0.0 25.14294 3 TCAACGC 12705 0.0 23.005566 4 ATCAACG 12725 0.0 22.968468 3 CATGGGG 2795 0.0 22.955376 4 TATCAAC 12715 0.0 22.950094 2 GAGTACT 6130 0.0 22.93821 12-13 GTGTAGG 460 0.0 22.73919 1 CAACGCA 13035 0.0 22.42498 5 ATGGGAG 1870 0.0 22.367067 5 AGTACTT 6185 0.0 22.235 12-13 AACGCAG 13260 0.0 22.069479 6 TATAGGG 130 2.9307377E-4 21.935137 2 GTACTTT 6430 0.0 21.645477 14-15 CATGGGA 5735 0.0 21.380543 4 GTATAGG 360 0.0 21.131365 1 ACGCAGA 14510 0.0 20.038105 7 >>END_MODULE