FastQCFastQC Report
Thu 26 May 2016
SRR937277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227174
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112420.9160885090459869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91270.7437413113380824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65910.5370876501620797No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27100.22083257956899346No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24750.2016828909347819No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20580.16770237961364892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.1399964471215981No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13230.10780867260877432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC600.00395054131.6675078
GTATCAA120900.027.9634741
GGTATCA85450.027.711821
GTACACG1452.3157121E-522.9550271
AGTACTT80550.022.73337712-13
TATCAAC147950.022.6055952
ATCAACG147850.022.5566223
GAGTACT80650.022.4401512-13
TCAACGC149100.022.431244
GTACATG82900.022.2548661
CAACGCA151250.022.1752015
CTGGTCG2606.2573235E-1021.923669
AACGCAG154250.021.7394876
TACATGG84000.021.5478482
GTACTTT85000.021.09442914-15
ATGGGAG19050.020.698625
ACATGGG85450.020.5150473
ACGCAGA168000.019.905297
CGCAGAG168900.019.7148518
TACTTTT92300.019.58045214-15
Produced by FastQC (version 0.11.5)