##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937277_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1227174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07427390084862 31.0 31.0 34.0 31.0 34.0 2 32.28821585203076 33.0 31.0 34.0 31.0 34.0 3 32.35746846005538 34.0 31.0 34.0 30.0 34.0 4 36.004736899575775 37.0 35.0 37.0 35.0 37.0 5 35.89724847495139 37.0 35.0 37.0 35.0 37.0 6 35.98824535070006 37.0 35.0 37.0 35.0 37.0 7 35.93474763969902 37.0 35.0 37.0 35.0 37.0 8 35.91993311461944 37.0 35.0 37.0 35.0 37.0 9 37.59029037447012 39.0 37.0 39.0 35.0 39.0 10-11 37.51519344445042 39.0 37.0 39.0 35.0 39.0 12-13 37.427042130944756 39.0 37.0 39.0 34.0 39.0 14-15 38.5694379933082 40.0 38.0 41.0 34.0 41.0 16-17 38.56014591247859 40.0 38.0 41.0 34.0 41.0 18-19 38.61129065641873 40.0 38.0 41.0 34.0 41.0 20-21 38.5268820884406 40.0 38.0 41.0 34.0 41.0 22-23 38.40261486961099 40.0 38.0 41.0 34.0 41.0 24-25 38.25255464995185 40.0 38.0 41.0 34.0 41.0 26-27 38.17373534641379 40.0 38.0 41.0 33.5 41.0 28-29 38.0629914747216 40.0 37.0 41.0 33.0 41.0 30-31 38.0782554063238 40.0 37.0 41.0 34.0 41.0 32-33 37.956158213912616 40.0 37.0 41.0 33.0 41.0 34-35 37.816076611792624 40.0 37.0 41.0 33.0 41.0 36-37 37.6301612485271 40.0 36.5 41.0 33.0 41.0 38-39 37.48073826531527 40.0 36.0 41.0 33.0 41.0 40-41 37.27569725238638 40.0 36.0 41.0 32.5 41.0 42-43 37.02390207093697 40.0 36.0 41.0 31.5 41.0 44-45 36.64572668586525 39.0 35.0 41.0 31.0 41.0 46-47 36.16762781806003 39.0 35.0 41.0 30.0 41.0 48-49 35.76763890043303 39.0 35.0 41.0 28.5 41.0 50-51 35.51595820967523 39.0 35.0 40.5 28.0 41.0 52-53 35.25484976050666 39.0 35.0 40.0 27.0 41.0 54-55 35.00761464959329 38.0 35.0 40.0 26.5 41.0 56-57 34.75506366660311 38.0 34.0 40.0 26.0 41.0 58-59 34.49702690897949 38.0 34.0 40.0 26.0 41.0 60-61 34.16102076804104 37.5 34.0 40.0 25.5 41.0 62-63 33.774446411022396 37.0 33.5 40.0 23.5 41.0 64-65 33.39772925436817 36.0 33.0 39.0 23.0 41.0 66-67 32.958417877171456 36.0 32.5 39.0 22.0 40.0 68-69 32.57336530923895 35.0 32.0 38.5 21.5 40.0 70-71 32.16815545309793 35.0 32.0 38.0 20.0 40.0 72-73 31.724263225915802 35.0 31.0 37.0 18.5 39.0 74-75 31.286907969041067 35.0 31.0 36.5 18.0 39.0 76-77 29.955855893296306 33.5 29.5 35.0 14.0 37.5 78-79 30.597590072801413 34.0 31.0 36.0 14.0 37.5 80-81 30.48050520953019 34.0 31.0 35.0 11.5 37.0 82-83 30.175810031829226 34.0 30.5 35.0 7.5 37.0 84-85 29.829407647163322 34.0 30.0 35.0 4.5 36.0 86-87 29.45210377664455 34.0 30.0 35.0 2.0 36.0 88-89 29.056194150136818 34.0 29.0 35.0 2.0 35.5 90-91 28.617394925251027 34.0 29.0 35.0 2.0 35.0 92-93 28.2660266596261 34.0 29.0 35.0 2.0 35.0 94-95 27.851266405578997 33.0 28.0 35.0 2.0 35.0 96-97 27.357366600009453 33.0 27.0 35.0 2.0 35.0 98-99 26.838547345364226 33.0 26.5 35.0 2.0 35.0 100-101 24.9328750446147 31.5 21.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24378848685999088 1101 2 0.20625645424522787 1101 3 0.1912787309687758 1101 4 0.1851987333579217 1101 5 0.1200073697395041 1101 6 0.10096530656790748 1101 7 0.14274414963583126 1101 8 0.16686218883144477 1101 9 0.20394152204988103 1101 10-11 0.20149477480260458 1101 12-13 0.19260728987082132 1101 14-15 0.16752862502958266 1101 16-17 0.23772843250299047 1101 18-19 0.28281592045026116 1101 20-21 0.24514888472263863 1101 22-23 0.2248543748490448 1101 24-25 0.33038907085165903 1101 26-27 0.11132447622858166 1101 28-29 0.24559960052276608 1101 30-31 0.4658469039313218 1101 32-33 0.36515787028712055 1101 34-35 0.33596872425127344 1101 36-37 0.2963705947600275 1101 38-39 0.38252910245243754 1101 40-41 0.4493762766420346 1101 42-43 0.5144491921913641 1101 44-45 0.6125328849592862 1101 46-47 0.6065387027661586 1101 48-49 0.5940285800071692 1101 50-51 0.2869619443523419 1101 52-53 0.37648568698284635 1101 54-55 0.542845909608964 1101 56-57 0.6265673515233701 1101 58-59 0.48869411003894214 1101 60-61 0.3933898394027793 1101 62-63 0.5011944955673897 1101 64-65 0.6682538793086579 1101 66-67 0.46899445474254975 1101 68-69 0.5027276661053008 1101 70-71 0.5282815490557482 1101 72-73 0.6232427183072993 1101 74-75 0.5134778958130113 1101 76-77 0.4398893138986075 1101 78-79 0.4464363431114613 1101 80-81 0.39081107657192504 1101 82-83 0.510968442565293 1101 84-85 0.3277592967080061 1101 86-87 0.30890467682872824 1101 88-89 0.3257530275968641 1101 90-91 0.38655460256608265 1101 92-93 0.07065620788062432 1101 94-95 0.19440127019574405 1101 96-97 0.315397746306072 1101 98-99 0.13037757520781312 1101 100-101 0.3358354986108196 1102 1 0.22544521277068696 1102 2 0.20502882421112645 1102 3 0.20383647202503852 1102 4 0.13603933343392782 1102 5 0.10707872769422266 1102 6 0.09940987319491512 1102 7 0.12663215843297593 1102 8 0.13782612654619442 1102 9 0.2377242028020845 1102 10-11 0.2800800748471417 1102 12-13 0.20886228691274766 1102 14-15 0.2093317131721193 1102 16-17 0.20239162436720193 1102 18-19 0.2831234809534777 1102 20-21 0.26071627834653555 1102 22-23 0.27749880748154254 1102 24-25 0.3332782458973398 1102 26-27 0.24896824933643558 1102 28-29 0.28989386344193235 1102 30-31 0.30216464070393556 1102 32-33 0.3823744020233164 1102 34-35 0.3502134771123693 1102 36-37 0.38197232284519345 1102 38-39 0.2534408658907452 1102 40-41 0.37110441405301486 1102 42-43 0.5694143679840664 1102 44-45 0.6353370923347299 1102 46-47 0.48990372810888516 1102 48-49 0.4952484394043566 1102 50-51 0.3874091804216704 1102 52-53 0.44365435420760946 1102 54-55 0.441384899269444 1102 56-57 0.4716250958446082 1102 58-59 0.5100525871508168 1102 60-61 0.47301536000281175 1102 62-63 0.4257896062042761 1102 64-65 0.3642959478391461 1102 66-67 0.5699333958202359 1102 68-69 0.4873849026578583 1102 70-71 0.4228973465647101 1102 72-73 0.4638130830570866 1102 74-75 0.4611191483613233 1102 76-77 0.33020579430941766 1102 78-79 0.21157504571547392 1102 80-81 0.44965993833686824 1102 82-83 0.6181771122453128 1102 84-85 0.4248488340351422 1102 86-87 0.5697714881119502 1102 88-89 0.3902894731899984 1102 90-91 0.41884878190193575 1102 92-93 0.5870362534074154 1102 94-95 0.585247329307439 1102 96-97 0.5766021558486791 1102 98-99 0.5393674185282613 1102 100-101 1.1391681876560504 1103 1 0.1325167920605992 1103 2 0.1883847271868646 1103 3 0.17003133280856275 1103 4 0.1086886421879143 1103 5 0.03904502825011491 1103 6 -0.02329958568456192 1103 7 0.08516316297210125 1103 8 0.09635713108531974 1103 9 0.023323276272350313 1103 10-11 0.056851044436413645 1103 12-13 -0.0015910874516436024 1103 14-15 0.05219575595236847 1103 16-17 0.06663426446446152 1103 18-19 0.07589765086252953 1103 20-21 0.020771086064712563 1103 22-23 0.10844556661327687 1103 24-25 0.06630521288331437 1103 26-27 0.14550170600207935 1103 28-29 -0.1363402390528563 1103 30-31 0.014102871201266964 1103 32-33 -0.06758729150699594 1103 34-35 0.018695964393721454 1103 36-37 -0.013331547389398679 1103 38-39 0.11182511437289833 1103 40-41 0.1868842453764401 1103 42-43 0.22577554802832367 1103 44-45 -0.041200323093157465 1103 46-47 0.12330504795253461 1103 48-49 0.3753953107650787 1103 50-51 0.054729341795685116 1103 52-53 0.07569060570815367 1103 54-55 0.11667671385588108 1103 56-57 0.011215280676523776 1103 58-59 0.12253684498539741 1103 60-61 0.15137521533502962 1103 62-63 0.2001805141188555 1103 64-65 0.16144173150111385 1103 66-67 0.20498868397071845 1103 68-69 0.24242335646012236 1103 70-71 0.17380152017629058 1103 72-73 0.14475382077050725 1103 74-75 0.32205673443877814 1103 76-77 0.23963917884616137 1103 78-79 0.22659656267729744 1103 80-81 0.257209643863888 1103 82-83 0.3296602131988777 1103 84-85 0.37380193999801037 1103 86-87 0.2684023841374348 1103 88-89 0.07974827579893784 1103 90-91 0.2635359051629287 1103 92-93 0.2784289562171125 1103 94-95 0.2431827258947017 1103 96-97 0.2891406064480613 1103 98-99 0.29631222774538557 1103 100-101 0.6224116694707256 1104 1 0.1292943117227736 1104 2 0.1673813902771002 1104 3 0.25828472139613723 1104 4 0.23994165672660728 1104 5 0.11429037478671233 1104 6 0.1244152709464359 1104 7 0.1284014911704645 1104 8 0.09068241580542491 1104 9 0.15311946884846606 1104 10-11 0.11686681847151448 1104 12-13 0.16436849558228772 1104 14-15 0.14693778526599033 1104 16-17 0.15238804678769924 1104 18-19 0.143823205291568 1104 20-21 0.03486682289179299 1104 22-23 0.14872226435225144 1104 24-25 0.043358421336812114 1104 26-27 0.11150509212880166 1104 28-29 0.1981615868962976 1104 30-31 0.19313570875577568 1104 32-33 0.06403792627804705 1104 34-35 0.16163800650375038 1104 36-37 0.13723481675703653 1104 38-39 0.014600089518339132 1104 40-41 0.04884421230264735 1104 42-43 0.04892425214580953 1104 44-45 0.1789612807890677 1104 46-47 0.20232533518270657 1104 48-49 -0.022002711697538757 1104 50-51 0.10710049179913028 1104 52-53 0.2275089262286727 1104 54-55 0.20500700394063642 1104 56-57 0.34720284355118736 1104 58-59 0.24372376114921934 1104 60-61 0.39778545344667293 1104 62-63 0.27083145760794025 1104 64-65 0.22399771579106442 1104 66-67 0.17662372053892028 1104 68-69 0.18902418724237435 1104 70-71 0.3275928255084608 1104 72-73 0.11409903835868107 1104 74-75 0.21758389727800775 1104 76-77 0.16770428796015935 1104 78-79 0.16784258840792177 1104 80-81 0.25354236526637663 1104 82-83 0.39733783720100035 1104 84-85 0.3929860950904889 1104 86-87 0.5518299269315783 1104 88-89 0.6467319406905148 1104 90-91 0.5687013479679024 1104 92-93 0.3298908229179034 1104 94-95 0.2605280123866187 1104 96-97 0.5114437632237632 1104 98-99 0.9475190275603325 1104 100-101 1.4762649881702181 1105 1 0.07681853532525906 1105 2 0.17043480642616515 1105 3 0.08615304437750382 1105 4 0.1009764393353052 1105 5 0.053537579755662534 1105 6 0.05783638895886156 1105 7 0.16207726756798024 1105 8 0.120471235681201 1105 9 0.12916543158138438 1105 10-11 0.11507861971375632 1105 12-13 0.11477594899844235 1105 14-15 0.19630859271940437 1105 16-17 0.20976009647714022 1105 18-19 0.12116357796237764 1105 20-21 0.26188297196011945 1105 22-23 0.19209120845162886 1105 24-25 0.09567953934923423 1105 26-27 0.16837714181824737 1105 28-29 0.2523963695049929 1105 30-31 0.12760651620919106 1105 32-33 0.24630811261344832 1105 34-35 0.33120943507518064 1105 36-37 0.24063295340300073 1105 38-39 0.13827772926988757 1105 40-41 0.2440702992591639 1105 42-43 0.15492673661785972 1105 44-45 0.3033283615344118 1105 46-47 0.24800235381624702 1105 48-49 0.14746250569376684 1105 50-51 0.11333092658173882 1105 52-53 0.20884432995538305 1105 54-55 0.1969156995928074 1105 56-57 0.12933762615988798 1105 58-59 0.134257922639911 1105 60-61 0.2526208571733761 1105 62-63 0.28584263773216634 1105 64-65 0.176215107095409 1105 66-67 0.14632434165692842 1105 68-69 0.4029260505964132 1105 70-71 0.33094003047740017 1105 72-73 0.42890152283073135 1105 74-75 0.2088484935512973 1105 76-77 0.2848030457241322 1105 78-79 0.3654612219482978 1105 80-81 0.21596907334091142 1105 82-83 0.23224404838112633 1105 84-85 0.45440162304079834 1105 86-87 0.49345911947816035 1105 88-89 0.4357698288892742 1105 90-91 0.32185476411697067 1105 92-93 0.17533989124088123 1105 94-95 0.10542614903257785 1105 96-97 0.2999431421057501 1105 98-99 0.2831413878087794 1105 100-101 0.6816991496609006 1106 1 -0.07234965418404826 1106 2 0.029403334344948462 1106 3 0.04313984640796065 1106 4 0.027525339504506974 1106 5 -0.0073977840345236245 1106 6 -0.22115650444215618 1106 7 -0.035013764580916984 1106 8 0.03709390911605226 1106 9 -0.22736654811404833 1106 10-11 -0.19972273392414763 1106 12-13 -0.3501032388188179 1106 14-15 -0.3140419994971779 1106 16-17 -0.0908260287343623 1106 18-19 -0.06041137973643629 1106 20-21 -0.00156474377592275 1106 22-23 -0.07397376616766138 1106 24-25 0.15157260195498878 1106 26-27 0.045638732342780486 1106 28-29 0.10926472821903843 1106 30-31 0.027523944297179526 1106 32-33 -0.061556523596365764 1106 34-35 -0.123200040390131 1106 36-37 -0.06885537147010012 1106 38-39 -0.11002616243908392 1106 40-41 -0.15007250953948414 1106 42-43 -0.3099605730268067 1106 44-45 -0.10177079244190423 1106 46-47 -0.07158140252893475 1106 48-49 0.038023588604083614 1106 50-51 -0.1477411546365346 1106 52-53 -0.09059729441010234 1106 54-55 -0.12120409736151316 1106 56-57 -0.03811956504147673 1106 58-59 -0.04891635823995699 1106 60-61 -0.21590029341883366 1106 62-63 -0.21872724382451736 1106 64-65 -0.1750403920585697 1106 66-67 -0.2939138986137948 1106 68-69 -0.08510982080798613 1106 70-71 -0.13430260911651004 1106 72-73 0.011136379006703834 1106 74-75 0.06043631081018219 1106 76-77 -0.09987106595099604 1106 78-79 0.04092721179601355 1106 80-81 -0.023674243071948098 1106 82-83 -0.2584402155782648 1106 84-85 -0.31599160876968924 1106 86-87 -0.17902615970965385 1106 88-89 -0.013444384308694879 1106 90-91 -0.08545691100993125 1106 92-93 -0.1250472491990493 1106 94-95 -0.6019708052821393 1106 96-97 0.07694754142893245 1106 98-99 0.08642192926393832 1106 100-101 0.5083922122666529 1107 1 0.08838432070628244 1107 2 0.12763418434218465 1107 3 0.11069674577719013 1107 4 0.10501874104603104 1107 5 -0.1438720625460519 1107 6 0.012594709332113041 1107 7 0.0888293671014182 1107 8 -0.008063756542753708 1107 9 0.03373899301217875 1107 10-11 0.01636197896724667 1107 12-13 0.0777887017200598 1107 14-15 0.062474067058445826 1107 16-17 -0.014870074595954463 1107 18-19 0.123592193825516 1107 20-21 0.10142449606587434 1107 22-23 0.14179385230854535 1107 24-25 0.1347843605000918 1107 26-27 0.17373763948543086 1107 28-29 0.08773322798089112 1107 30-31 0.11421242600701476 1107 32-33 0.19311371136928557 1107 34-35 0.12889528266460104 1107 36-37 0.06799718357407158 1107 38-39 0.29523760485309225 1107 40-41 0.43302333191856945 1107 42-43 0.22820325294756572 1107 44-45 0.36445931021248157 1107 46-47 0.2903009541272894 1107 48-49 0.2582351339985891 1107 50-51 0.3023934460218669 1107 52-53 0.4408661028947307 1107 54-55 0.4533038799971649 1107 56-57 0.47365240065443714 1107 58-59 0.4387866940240457 1107 60-61 0.5848012615279714 1107 62-63 0.4757207092718261 1107 64-65 0.48925398734424164 1107 66-67 0.4862179652961274 1107 68-69 0.4661739510629772 1107 70-71 0.32594282985531464 1107 72-73 0.43840136730973356 1107 74-75 0.33762485436000844 1107 76-77 0.41467054183610585 1107 78-79 0.3757710197710047 1107 80-81 0.3750956674311148 1107 82-83 0.4883230530467557 1107 84-85 0.5752022451247818 1107 86-87 0.49574154560568573 1107 88-89 0.38647153962022074 1107 90-91 0.40832817002677047 1107 92-93 0.39757566106980846 1107 94-95 0.2123829141958744 1107 96-97 0.3396800005816445 1107 98-99 0.7721497859425277 1107 100-101 1.0860829754773853 1108 1 -0.07778534120022584 1108 2 -0.01601228617972339 1108 3 -0.1380525550481977 1108 4 -0.025441938266993702 1108 5 -0.07319206848554671 1108 6 -0.13217825569297048 1108 7 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-1.2274388379352743 2316 96-97 -1.4069677180158848 2316 98-99 -1.4716398459010378 2316 100-101 -1.8536860805736772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 13.0 5 41.0 6 65.0 7 150.0 8 345.0 9 722.0 10 1208.0 11 2040.0 12 3048.0 13 4583.0 14 5808.0 15 8360.0 16 11920.0 17 16590.0 18 14154.0 19 7612.0 20 6222.0 21 6251.0 22 6528.0 23 6954.0 24 8276.0 25 9602.0 26 11777.0 27 14642.0 28 18047.0 29 22760.0 30 28282.0 31 36076.0 32 45996.0 33 60367.0 34 82766.0 35 120340.0 36 180807.0 37 265862.0 38 202162.0 39 16793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.934180550957805 19.385851476542793 15.485557456259794 21.194410516239607 2 15.891389485109691 21.361436927444682 39.17977401737651 23.567399570069117 3 19.31062750677573 25.765376385092903 30.47049562653707 24.453500481594297 4 13.050553548233584 17.358500098600523 36.711582872518484 32.879363480647406 5 13.447193831809217 38.1485647219913 33.19428822925327 15.209953216946223 6 31.234364482950255 36.779788359270974 16.85987480178035 15.125972355998416 7 28.340805786302514 34.15171768632647 19.835980879647057 17.671495647723958 8 27.30435944698959 33.88036252397785 19.158081901995967 19.65719612703659 9 27.638867837812732 16.19680664681618 19.778857765891388 36.3854677494797 10-11 25.290015922762375 27.120440948064417 26.17680948259986 21.412733646573344 12-13 26.95937320593073 24.445628776331183 27.418491957069058 21.17650606066903 14-15 23.599954040747278 25.124717440232597 26.879929007622387 24.39539951139773 16-17 21.646971008186288 29.006440814424035 27.20082889630973 22.145759281079943 18-19 21.617798291032894 26.300345346299707 31.2480544731228 20.833801889544596 20-21 22.553280952823314 25.259172700855785 31.26948582678577 20.91806051953513 22-23 22.247818749424486 25.204516433432232 31.188073629990477 21.35959118715281 24-25 22.090723112909462 25.478711852887958 31.3168431358728 21.11372189832978 26-27 22.043295087845106 25.610800547605855 31.277706248573946 21.068198115975097 28-29 21.51527177575699 25.550694179033567 31.773491544926262 21.16054250028318 30-31 21.517812469951288 25.692811288374756 31.538111139903553 21.251265101770407 32-33 21.60402681282361 25.80457213076548 31.108872906368617 21.48252815004229 34-35 22.092425361032745 25.459836991331304 30.998701080694342 21.449036566941608 36-37 22.229977167052105 25.271925578605803 31.000004889282206 21.498092365059883 38-39 22.105381958874617 25.401491557024514 31.258362709770577 21.23476377433029 40-41 21.848409435010847 25.51308942334176 31.444196177559174 21.19430496408822 42-43 21.926033443791187 25.731898739541688 30.74231426862285 21.599753548044276 44-45 21.97288241113322 25.98840914165391 30.13570202758533 21.903006419627534 46-47 22.10069639676199 26.159941458994407 29.53778355799585 22.201578586247752 48-49 22.485768114383127 26.36985464164006 29.10284116188902 22.04153608208779 50-51 22.575445698816957 26.61933026612363 28.74457900835578 22.06064502670363 52-53 22.676124168210865 26.830017585118327 28.591503731337202 21.902354515333606 54-55 22.060033866428068 26.732639381212444 28.695400978182395 22.511925774177094 56-57 22.40774331920331 27.25273677571396 28.257036084532427 22.082483820550305 58-59 22.549980687335292 26.474851976981263 28.578750853587188 22.396416482096264 60-61 22.446857576839143 26.450650030069085 29.053826107789117 22.048666285302655 62-63 22.662474922056695 26.475137185109855 28.9255231939399 21.936864698893555 64-65 22.547536046233052 26.444497683295115 28.96198909038164 22.04597718009019 66-67 22.23771853054253 26.940882059104904 28.774933302041926 22.04646610831064 68-69 22.202800906798874 26.823498542179024 28.859762348289646 22.113938202732456 70-71 22.081587452146152 27.028685418693684 28.641618873933115 22.248108255227052 72-73 22.40075360197788 26.869968618931477 28.22674553628543 22.502532242805213 74-75 22.28453340765042 27.276857234589393 27.99297410147216 22.445635256288025 76-77 22.41389566597728 26.91549853565998 28.54460736619257 22.125998432170174 78-79 22.268154312265416 26.783569404175772 28.52370568476842 22.424570598790392 80-81 21.86645903514905 27.651987411728086 28.22517426216657 22.256379290956293 82-83 22.07044579379964 27.383358710138484 28.02744198951979 22.518753506542083 84-85 22.067614765172863 27.141248315212874 27.79079792496158 23.000338994652687 86-87 22.28249620673189 26.86652422557844 28.08692980783491 22.764049759854757 88-89 22.349683093025114 26.580990144836836 28.176199952085035 22.893126810053015 90-91 22.033590277180707 26.930206270885943 28.497907382045497 22.538296069887856 92-93 21.9160015156886 26.805360322369037 28.346228909722814 22.932409252219546 94-95 21.717498903985906 26.671727073748304 28.17974468168328 23.43102934058251 96-97 22.06875308635939 26.615052144194713 28.049689775044122 23.26650499440177 98-99 21.86926527006809 26.54941605577702 28.07454863702888 23.50677003712601 100-101 21.477798989835193 26.63302167229341 28.649724639423326 23.23945469844808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 929.0 1 668.0 2 556.0 3 713.5 4 574.5 5 497.5 6 1033.0 7 2456.0 8 4008.0 9 4511.5 10 3686.0 11 2795.0 12 2645.5 13 2758.0 14 2868.0 15 2957.5 16 3012.0 17 3035.0 18 3154.0 19 3230.0 20 3341.0 21 3655.0 22 4088.5 23 4585.5 24 5185.0 25 6026.5 26 7111.5 27 8335.5 28 9467.5 29 10681.5 30 12189.5 31 13804.0 32 15767.5 33 18077.5 34 20845.5 35 22849.0 36 25556.5 37 28832.0 38 30993.5 39 31414.5 40 31559.5 41 34262.0 42 37218.0 43 39418.0 44 41506.5 45 43734.5 46 44103.0 47 45268.0 48 55301.0 49 56219.0 50 48401.5 51 45613.0 52 42493.0 53 41686.0 54 40903.5 55 37994.5 56 34287.0 57 30469.0 58 27237.0 59 24447.5 60 21481.0 61 17756.5 62 14252.5 63 11166.0 64 8160.0 65 6190.0 66 4691.0 67 3698.5 68 2606.0 69 1606.0 70 1161.0 71 901.5 72 748.0 73 665.5 74 546.5 75 393.5 76 261.0 77 141.0 78 76.5 79 50.5 80 32.5 81 16.0 82 8.5 83 4.5 84 4.0 85 2.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05932329074768533 2 0.0 3 0.0 4 0.0 5 0.019068200597470285 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033002654880237036 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.963684041545861E-4 24-25 4.0744018370663004E-5 26-27 0.001140832514378564 28-29 0.0033410095063943664 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01303808587861216 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.148803674132601E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.85208128594641E-4 84-85 0.0013038085878612161 86-87 0.0 88-89 0.0 90-91 0.009982284500812436 92-93 7.333923306719341E-4 94-95 0.0 96-97 0.0 98-99 1.6297607348265202E-4 100-101 0.01193799738260426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1227174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.20389813958973 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65996724540628 33.109698272930885 2 14.4774526060586 13.378294910621392 3 5.559409883938195 7.705992239811262 4 2.6453819959957037 4.889078411331603 5 1.4756231744822716 3.4089771423098463 6 0.9160303854432226 2.5394504773072666 7 0.5899498892963014 1.9080589214755985 8 0.3960535012978791 1.4639372505436061 9 0.30862997286677796 1.2833917046244858 >10 1.733935283278908 14.584301766805002 >50 0.1545726925349123 4.928775178631234 >100 0.0767998383624219 6.2187829197138775 >500 0.00442395074179462 1.3593668283399123 >1k 0.0012387062077024937 1.0176037258221189 >5k 3.5391605934356957E-4 1.2851273792762385 >10k+ 1.7695802967178479E-4 0.9191628704557497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11242 0.9160885090459869 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9127 0.7437413113380824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6591 0.5370876501620797 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2710 0.22083257956899346 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2475 0.2016828909347819 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2058 0.16770237961364892 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1718 0.1399964471215981 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1323 0.10780867260877432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.148803674132601E-5 0.0 22-23 0.0 0.0 0.0 1.22232055111989E-4 0.0 24-25 0.0 0.0 0.0 1.6297607348265202E-4 0.0 26-27 0.0 0.0 0.0 2.85208128594641E-4 0.0 28-29 0.0 0.0 0.0 0.001385296624602542 0.0 30-31 0.0 0.0 0.0 0.003870681745212985 0.0 32-33 0.0 0.0 0.0 0.006274578829082103 0.0 34-35 0.0 0.0 0.0 0.00896368404154586 0.0 36-37 0.0 0.0 0.0 0.011367581125414979 0.0 38-39 0.0 0.0 0.0 0.012956597841870835 0.0 40-41 0.0 0.0 0.0 0.015808679127817245 0.0 42-43 0.0 0.0 0.0 0.019434896762806252 0.0 44-45 0.0 0.0 0.0 0.022409210103864652 0.0 46-47 0.0 0.0 0.0 0.02725774828997355 0.0 48-49 0.0 0.0 0.0 0.03341009506394366 0.0 50-51 0.0 0.0 0.0 0.04278121928919615 0.0 52-53 0.0 0.0 0.0 0.06547563752165544 0.0 54-55 4.0744018370663004E-5 0.0 0.0 0.08955535237871728 0.0 56-57 8.148803674132601E-5 0.0 0.0 0.12353586369985022 0.0 58-59 8.148803674132601E-5 0.0 0.0 0.17617713543474683 0.0 60-61 8.148803674132601E-5 0.0 0.0 0.25151282540210274 0.0 62-63 8.148803674132601E-5 0.0 0.0 0.3071284104780577 0.0 64-65 8.148803674132601E-5 0.0 0.0 0.3613586989294102 0.0 66-67 8.148803674132601E-5 0.0 0.0 0.4684747232258832 0.0 68-69 8.148803674132601E-5 0.0 0.0 0.6065969455024308 0.0 70-71 8.148803674132601E-5 0.0 0.0 0.7315995938636248 0.0 72-73 8.148803674132601E-5 0.0 0.0 0.8456828453014813 0.0 74-75 8.148803674132601E-5 0.0 0.0 1.055840492057361 0.0 76-77 8.148803674132601E-5 0.0 0.0 1.2131938910048616 0.0 78-79 8.148803674132601E-5 0.0 0.0 1.3417005249459328 0.0 80-81 8.148803674132601E-5 0.0 0.0 1.4979538353974253 0.0 82-83 8.148803674132601E-5 0.0 0.0 1.768575605415369 0.0 84-85 8.148803674132601E-5 0.0 0.0 2.0405419280395445 0.0 86-87 8.148803674132601E-5 0.0 0.0 2.3869068282085504 0.0 88-89 8.148803674132601E-5 0.0 0.0 2.8068146815366037 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 60 0.003950541 31.667507 8 GTATCAA 12090 0.0 27.963474 1 GGTATCA 8545 0.0 27.71182 1 GTACACG 145 2.3157121E-5 22.955027 1 AGTACTT 8055 0.0 22.733377 12-13 TATCAAC 14795 0.0 22.605595 2 ATCAACG 14785 0.0 22.556622 3 GAGTACT 8065 0.0 22.44015 12-13 TCAACGC 14910 0.0 22.43124 4 GTACATG 8290 0.0 22.254866 1 CAACGCA 15125 0.0 22.175201 5 CTGGTCG 260 6.2573235E-10 21.92366 9 AACGCAG 15425 0.0 21.739487 6 TACATGG 8400 0.0 21.547848 2 GTACTTT 8500 0.0 21.094429 14-15 ATGGGAG 1905 0.0 20.69862 5 ACATGGG 8545 0.0 20.515047 3 ACGCAGA 16800 0.0 19.90529 7 CGCAGAG 16890 0.0 19.714851 8 TACTTTT 9230 0.0 19.580452 14-15 >>END_MODULE