FastQCFastQC Report
Thu 26 May 2016
SRR937276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260458
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81900.6497638160097362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60020.4761761201087224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.3496348152814294No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27710.21984072456202428No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22070.17509508448516334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18580.14740673628157383No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15320.12154312162721805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13620.10805596061114293No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.10432715727140451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107300.028.608661
GTACATG86750.026.6214371
GGTATCA76200.025.8796181
TACATGG88550.025.6448632
ACATGGG89150.024.8327963
AGTACTT62050.023.65516512-13
TATCAAC132150.023.1874832
TCAACGC131500.023.1242224
ATCAACG132450.022.991493
GAGTACT62200.022.91079312-13
CAACGCA133950.022.7685895
CATGGGG30600.022.5143554
AACGCAG135750.022.465796
CATGGGA60500.022.1465784
AGGACCG1954.2173997E-721.925615
GTACTTT65350.021.76837214-15
ATGGGAG21350.021.1382755
ATTCGCG1351.0059011E-921.10599580-81
ACGCAGA147350.020.471527
TACTTTT71300.020.35149814-15
Produced by FastQC (version 0.11.5)