##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937276_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1260458 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.720637260424386 33.0 31.0 34.0 30.0 34.0 2 31.79811624028726 33.0 31.0 34.0 30.0 34.0 3 31.83631029355996 33.0 31.0 34.0 30.0 34.0 4 35.46903347830709 37.0 35.0 37.0 33.0 37.0 5 35.48674926098291 37.0 35.0 37.0 33.0 37.0 6 35.525107540275044 37.0 35.0 37.0 33.0 37.0 7 35.42058838929976 37.0 35.0 37.0 33.0 37.0 8 35.426913074453886 37.0 35.0 37.0 33.0 37.0 9 37.03513326108446 39.0 37.0 39.0 33.0 39.0 10-11 36.92077958964123 39.0 37.0 39.0 33.0 39.0 12-13 36.768210444140145 39.0 37.0 39.0 32.5 39.0 14-15 37.899757072429225 40.0 38.0 41.0 33.0 41.0 16-17 37.838140977327285 40.0 38.0 41.0 33.0 41.0 18-19 37.829582580300176 40.0 38.0 41.0 33.0 41.0 20-21 37.74204138495689 40.0 37.5 41.0 32.0 41.0 22-23 37.57719614616274 40.0 37.0 41.0 32.0 41.0 24-25 37.50363399653142 40.0 37.0 41.0 32.0 41.0 26-27 37.52310628358899 40.0 37.0 41.0 32.0 41.0 28-29 37.43335438388269 40.0 37.0 41.0 32.0 41.0 30-31 37.194676855555684 39.0 36.5 41.0 31.5 41.0 32-33 36.95491083399844 39.0 36.0 41.0 31.0 41.0 34-35 36.951660031512354 39.0 36.0 41.0 31.0 41.0 36-37 36.798601778083835 39.0 35.5 41.0 30.5 41.0 38-39 36.67459010930948 39.0 35.0 41.0 30.0 41.0 40-41 36.425110555052214 39.0 35.0 41.0 30.0 41.0 42-43 36.130294305720625 39.0 35.0 41.0 30.0 41.0 44-45 35.84677553714602 39.0 35.0 40.5 28.5 41.0 46-47 35.43015475327222 39.0 35.0 40.0 26.5 41.0 48-49 35.05488996856698 38.5 35.0 40.0 25.5 41.0 50-51 34.044093496173616 37.5 33.5 39.5 23.5 40.5 52-53 34.145089721355255 38.0 33.5 39.5 24.0 40.5 54-55 34.49999603318794 38.0 34.0 40.0 24.0 41.0 56-57 34.28017752277347 38.0 34.0 40.0 23.0 41.0 58-59 33.98867475155856 38.0 34.0 40.0 22.5 41.0 60-61 33.66111167527994 37.0 33.0 40.0 22.0 41.0 62-63 33.32829138297349 37.0 33.0 40.0 20.5 41.0 64-65 32.88051843060221 36.0 32.0 39.0 19.0 41.0 66-67 32.51336577656693 36.0 32.0 39.0 18.0 40.5 68-69 32.03302291706665 35.0 31.5 38.5 14.5 40.0 70-71 31.565173135479327 35.0 31.0 38.0 10.0 40.0 72-73 31.094551742303196 35.0 31.0 37.0 7.5 39.5 74-75 30.604922179080937 35.0 30.0 36.5 6.5 39.0 76-77 30.13051843060221 34.0 30.0 36.0 2.0 39.0 78-79 29.640272424785277 34.0 29.0 35.5 2.0 37.5 80-81 29.16379760372817 34.0 29.0 35.0 2.0 37.0 82-83 28.703376074411047 34.0 29.0 35.0 2.0 36.5 84-85 28.189097137707087 34.0 27.5 35.0 2.0 36.0 86-87 27.728627609963997 33.0 26.5 35.0 2.0 36.0 88-89 27.25858061117467 33.0 26.0 35.0 2.0 35.0 90-91 26.736241509038777 33.0 25.0 35.0 2.0 35.0 92-93 26.230036224927765 32.0 24.5 35.0 2.0 35.0 94-95 25.667953236045946 32.0 22.5 34.5 2.0 35.0 96-97 24.89294724615973 32.0 19.0 34.0 2.0 35.0 98-99 23.936186291014856 31.0 2.0 34.0 2.0 35.0 100-101 21.378203002400713 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04674679329730225 1101 2 0.1229628646263734 1101 3 0.040681576430159794 1101 4 0.24077510330891272 1101 5 0.06402334424351608 1101 6 0.025194455911801583 1101 7 -0.034331631202704216 1101 8 0.009498407240620566 1101 9 0.1540258232919811 1101 10-11 -0.06491114542829735 1101 12-13 0.16872340918013862 1101 14-15 0.11094585753453856 1101 16-17 0.010599354954557327 1101 18-19 0.12397997890503376 1101 20-21 0.2850441260976666 1101 22-23 0.11229648062427344 1101 24-25 0.20710966302620903 1101 26-27 0.13245086520396399 1101 28-29 0.036158065507315484 1101 30-31 0.26235910465158696 1101 32-33 0.037563715564729705 1101 34-35 0.022083350840155447 1101 36-37 -0.014188011584458593 1101 38-39 -0.03289952873851831 1101 40-41 -0.13668684570344425 1101 42-43 -0.15159308547099926 1101 44-45 -0.085337608265462 1101 46-47 0.16587268973275116 1101 48-49 0.1940702966327521 1101 50-51 0.03188592626732145 1101 52-53 0.2353057950745594 1101 54-55 0.3114519651879277 1101 56-57 0.19603667702909888 1101 58-59 0.09065728115657379 1101 60-61 0.2124485247162724 1101 62-63 0.03290148518840397 1101 64-65 -0.105431152540163 1101 66-67 -0.21757453451990472 1101 68-69 -0.25195545606419145 1101 70-71 -0.33534443345832443 1101 72-73 -0.0932365182594026 1101 74-75 -0.34364221602575284 1101 76-77 -0.216069419839517 1101 78-79 -0.31748768403849326 1101 80-81 -0.4254040148205185 1101 82-83 -0.4482542240548817 1101 84-85 -0.4642226615354339 1101 86-87 -0.5259116970139885 1101 88-89 -0.7917676425610018 1101 90-91 -0.5756872391523551 1101 92-93 -0.29363364236689904 1101 94-95 -0.382179083366303 1101 96-97 -0.23451474917600024 1101 98-99 -0.1284873130467119 1101 100-101 0.0218317635605203 1102 1 0.03816304237633972 1102 2 0.016618642082285362 1102 3 -0.06721885943245809 1102 4 0.02343701010033783 1102 5 0.03640921588216628 1102 6 0.032738124564602344 1102 7 0.14499877254695548 1102 8 0.013895017086326789 1102 9 -0.1403934259384343 1102 10-11 0.04856451122836347 1102 12-13 0.10765655959603038 1102 14-15 0.1483737374368701 1102 16-17 0.020474271376514253 1102 18-19 0.1964606421446078 1102 20-21 0.15082687179197052 1102 22-23 0.04973290685244791 1102 24-25 0.20742693839050474 1102 26-27 0.03582850723356046 1102 28-29 0.13931556982377202 1102 30-31 0.06819859124642136 1102 32-33 0.018450947074647672 1102 34-35 0.18694504838623516 1102 36-37 0.08606402124014068 1102 38-39 0.19805487242378206 1102 40-41 0.19509939784003194 1102 42-43 0.14498592642849673 1102 44-45 0.3186229155293532 1102 46-47 0.22214884889103104 1102 48-49 0.255391301658328 1102 50-51 0.10866237505108955 1102 52-53 0.06869908302484617 1102 54-55 0.22515697618364072 1102 56-57 0.2992494811009436 1102 58-59 0.09604314218213972 1102 60-61 0.20406139514782495 1102 62-63 0.29674052826803177 1102 64-65 0.13806428355426448 1102 66-67 -0.006195967476216424 1102 68-69 0.059621799090557204 1102 70-71 0.024384106186854382 1102 72-73 -0.04611442366106644 1102 74-75 0.01914177475091705 1102 76-77 -0.057982221621173835 1102 78-79 -0.19992068335758262 1102 80-81 -0.28891974983526225 1102 82-83 -0.293415188236839 1102 84-85 -0.03195145589463877 1102 86-87 -8.893955722300007E-4 1102 88-89 -0.22336547592775702 1102 90-91 -0.08404951176003905 1102 92-93 -0.033460023478291134 1102 94-95 -0.1517832655918454 1102 96-97 0.04054092762318007 1102 98-99 0.1498371937381222 1102 100-101 0.0025894037244533763 1103 1 -0.027229456496037585 1103 2 0.029342810923314033 1103 3 0.0194511948647893 1103 4 0.0961759094249075 1103 5 -0.08127535764749183 1103 6 0.03263364090230425 1103 7 0.07871330947878619 1103 8 0.03372350401444635 1103 9 0.12367561321153886 1103 10-11 0.0754673414224385 1103 12-13 -0.008851002623138982 1103 14-15 -0.15272162817030477 1103 16-17 -0.08811051740091358 1103 18-19 -0.12132812957286632 1103 20-21 0.0077519119011313364 1103 22-23 0.018488295183395564 1103 24-25 -0.08743166609601616 1103 26-27 -0.20260206822591442 1103 28-29 -0.04791601278969182 1103 30-31 -0.21385676796612785 1103 32-33 -0.22066505262846192 1103 34-35 -0.3139156571925028 1103 36-37 -0.3231299700928574 1103 38-39 -0.3579777744385666 1103 40-41 -0.19266088352362232 1103 42-43 -0.0436701738758174 1103 44-45 -0.06615387196914924 1103 46-47 -0.16313665414356393 1103 48-49 -0.3055101125822901 1103 50-51 -0.4343914597276921 1103 52-53 -0.3533743752701852 1103 54-55 -0.40874483201175593 1103 56-57 -0.48645566942975904 1103 58-59 -0.518569440391353 1103 60-61 -0.42083169465673365 1103 62-63 -0.4637278211755742 1103 64-65 -0.3164813267045403 1103 66-67 -0.2718565268920159 1103 68-69 -0.1283734277760189 1103 70-71 -0.2333388351138801 1103 72-73 -0.3527537038086024 1103 74-75 -0.2586331800013788 1103 76-77 -0.2328576120075354 1103 78-79 -0.13927191403809402 1103 80-81 -0.19337943227344212 1103 82-83 -0.29937104246214474 1103 84-85 -0.47759996828708395 1103 86-87 -0.15484692838752423 1103 88-89 -0.17205115138893845 1103 90-91 -0.20268357343394783 1103 92-93 -0.41168260222643127 1103 94-95 -0.49376799841627417 1103 96-97 -0.3504560634757219 1103 98-99 -0.14032849565421657 1103 100-101 0.2628302300018106 1104 1 0.02218307914270312 1104 2 0.13576238233874705 1104 3 0.03055668552973856 1104 4 -0.05229392410770117 1104 5 0.021056105610114173 1104 6 0.055294130187689916 1104 7 -0.0010438872470501792 1104 8 -0.11111117822596128 1104 9 4.023675571644958E-4 1104 10-11 0.04912743983133794 1104 12-13 0.03897560916753662 1104 14-15 0.11977561270158077 1104 16-17 0.1897147148257048 1104 18-19 0.11229816951662741 1104 20-21 0.0698960993379032 1104 22-23 0.032807595284559454 1104 24-25 0.08763156392330274 1104 26-27 0.11982849371651128 1104 28-29 0.19170679140418656 1104 30-31 0.09443038829250128 1104 32-33 0.050142222272668846 1104 34-35 0.3041319147747501 1104 36-37 0.11717744276467812 1104 38-39 0.3060110051071021 1104 40-41 0.3529894659659405 1104 42-43 0.3888013151336551 1104 44-45 0.3346727168287913 1104 46-47 0.19285065386711153 1104 48-49 0.3037645816143453 1104 50-51 0.3133811191373823 1104 52-53 0.30842894488064587 1104 54-55 0.27412500611939095 1104 56-57 0.36092775740736727 1104 58-59 0.3354652096592332 1104 60-61 0.28046452073919426 1104 62-63 0.4392572105543948 1104 64-65 0.6366951195384445 1104 66-67 0.40281122809739855 1104 68-69 0.2771735693728843 1104 70-71 0.3307051269940935 1104 72-73 0.3066221584189357 1104 74-75 0.18420573290214648 1104 76-77 0.4004548362588132 1104 78-79 0.3235356137831147 1104 80-81 0.5261299027766846 1104 82-83 0.6713684038728154 1104 84-85 0.8407143193980069 1104 86-87 0.7376436445914152 1104 88-89 0.5691098667293346 1104 90-91 0.7076395894465826 1104 92-93 0.8732991185498058 1104 94-95 0.9543914332033339 1104 96-97 0.9088375997267057 1104 98-99 1.072875082015237 1104 100-101 1.3909969043327237 1105 1 0.10665471285588524 1105 2 0.02521182797682897 1105 3 -0.06865959267563326 1105 4 0.018875063354563792 1105 5 0.05529463988264638 1105 6 0.17374932795392795 1105 7 0.2085756291934331 1105 8 -0.055669887102098414 1105 9 0.06734961478535695 1105 10-11 -0.027977537640623495 1105 12-13 0.03611852620816336 1105 14-15 0.15737578766781013 1105 16-17 -0.07291169374310869 1105 18-19 0.05553244975269678 1105 20-21 0.17801452110624183 1105 22-23 0.15463094965010526 1105 24-25 0.07589896762388548 1105 26-27 0.1374016078434579 1105 28-29 0.0998209070088123 1105 30-31 0.2210024213457089 1105 32-33 0.11900174614694237 1105 34-35 -0.01282978076587682 1105 36-37 0.10145144622774893 1105 38-39 0.20444446807861993 1105 40-41 0.14660212200771383 1105 42-43 0.09493113917020679 1105 44-45 0.133229812026741 1105 46-47 0.05617075141304895 1105 48-49 0.017569859157930523 1105 50-51 -0.026992508556233474 1105 52-53 -0.16745257215816167 1105 54-55 0.08768823193462083 1105 56-57 -0.00488311588263457 1105 58-59 0.16853875666893714 1105 60-61 0.29479016417071335 1105 62-63 0.21443948593130102 1105 64-65 0.16828366332986633 1105 66-67 0.19556746750197362 1105 68-69 -0.07709168577864745 1105 70-71 -0.019855127568174424 1105 72-73 0.17050404034845812 1105 74-75 0.060955961808740966 1105 76-77 0.03172058775237119 1105 78-79 0.21030418921350247 1105 80-81 0.2615014113844758 1105 82-83 0.2681482999809468 1105 84-85 0.4633416798556418 1105 86-87 0.19626594417349352 1105 88-89 0.44018757485302373 1105 90-91 0.6143791322679775 1105 92-93 0.643733281839534 1105 94-95 0.5239127464472801 1105 96-97 0.5181167025941562 1105 98-99 0.7703835130304952 1105 100-101 1.283204844445315 1106 1 -0.06831832740514798 1106 2 -0.06088596219144904 1106 3 -0.042930812850880073 1106 4 0.1027189846702683 1106 5 0.0993369710071903 1106 6 -0.10223344555306113 1106 7 -0.4766143719508378 1106 8 -0.26143250963454534 1106 9 -0.40305015219927753 1106 10-11 -0.5896702624987213 1106 12-13 -0.3583180585910597 1106 14-15 -0.4026794147816233 1106 16-17 -0.14915400891719344 1106 18-19 -0.5155953168713197 1106 20-21 -0.3720577760715642 1106 22-23 -0.2690235773321703 1106 24-25 -0.35445128359580025 1106 26-27 -0.4986211111545771 1106 28-29 -0.1554323411629568 1106 30-31 -0.2270519697109421 1106 32-33 -0.13562735372718038 1106 34-35 -0.11845301017851995 1106 36-37 -0.38904676281338624 1106 38-39 -0.31685372570555614 1106 40-41 -0.1124419601995541 1106 42-43 -0.07825701033410581 1106 44-45 0.07212135932504538 1106 46-47 -0.18438640496730585 1106 48-49 0.002821755008383775 1106 50-51 -0.12880756648065983 1106 52-53 0.20060247772169504 1106 54-55 0.048423235904891726 1106 56-57 0.0346826849516475 1106 58-59 0.08275910875793357 1106 60-61 -0.15080144172818422 1106 62-63 -0.1234527305926818 1106 64-65 -0.07260637214256604 1106 66-67 0.07147551658777473 1106 68-69 -0.08013700934753132 1106 70-71 0.09856921005444619 1106 72-73 -0.04596966046952389 1106 74-75 0.1667499651305988 1106 76-77 0.4056339629036394 1106 78-79 0.31669893527183746 1106 80-81 -0.11790013442280767 1106 82-83 -0.012297133854129783 1106 84-85 -0.09420566005898934 1106 86-87 0.0020561332557527123 1106 88-89 0.3189415668015094 1106 90-91 0.41502963349373445 1106 92-93 0.5936661858975363 1106 94-95 0.605897225959616 1106 96-97 0.48523535665302475 1106 98-99 0.41104226688374723 1106 100-101 0.9475612955380939 1107 1 0.15367864597978453 1107 2 0.06348216354070502 1107 3 0.029689716168615377 1107 4 0.030548573479087793 1107 5 0.07748061671090056 1107 6 0.02527866774444476 1107 7 0.12928752743243876 1107 8 0.1697712535784035 1107 9 0.252967524326678 1107 10-11 0.10739872973874043 1107 12-13 0.14324766772818265 1107 14-15 0.12193633273304272 1107 16-17 0.2745738099599535 1107 18-19 0.06197707802900965 1107 20-21 0.08865548405778867 1107 22-23 0.24353812869416203 1107 24-25 0.03321582977007864 1107 26-27 0.026425951798103142 1107 28-29 0.07759194924623358 1107 30-31 -0.05075904898182415 1107 32-33 0.029212062464296196 1107 34-35 0.12435308134012502 1107 36-37 -0.16546127233309704 1107 38-39 -0.01493747724963157 1107 40-41 -0.0874968859699976 1107 42-43 -0.157615062543222 1107 44-45 0.02246766516814347 1107 46-47 0.05601696897918629 1107 48-49 0.3471208702704516 1107 50-51 0.274708252597037 1107 52-53 0.08518656884832865 1107 54-55 0.023761906837989955 1107 56-57 0.21645222918169793 1107 58-59 -0.04688280256772259 1107 60-61 0.33408143647334754 1107 62-63 0.42282166123545295 1107 64-65 0.4661635868796168 1107 66-67 0.3905418240718106 1107 68-69 0.3254892355986172 1107 70-71 0.3849398701209026 1107 72-73 0.311429592904652 1107 74-75 0.3963875462087856 1107 76-77 0.14158515873566202 1107 78-79 0.12680618866423998 1107 80-81 0.3118678098947676 1107 82-83 0.3222657618507192 1107 84-85 0.38962770290235227 1107 86-87 0.5578815695851596 1107 88-89 0.4876896010970704 1107 90-91 0.6736166281652523 1107 92-93 0.7903169673932489 1107 94-95 0.7977716305505886 1107 96-97 0.7705872690270503 1107 98-99 1.0415502277018405 1107 100-101 1.6012246848911573 1108 1 -0.08412376534911559 1108 2 -0.0941187083765449 1108 3 -0.09908807189007973 1108 4 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1.3593472588965767 2310 80-81 1.3915070593246348 2310 82-83 1.3558275116977576 2310 84-85 1.3850110049175903 2310 86-87 1.3888965780857845 2310 88-89 1.5345624472224557 2310 90-91 1.4767984375529402 2310 92-93 1.6152654557813726 2310 94-95 1.9509062209607215 2310 96-97 1.7357123358349504 2310 98-99 1.8602759078123157 2310 100-101 1.9874275535800763 2311 1 0.4432513042979629 2311 2 0.3935628148379777 2311 3 0.2791079196439128 2311 4 0.3261622081134945 2311 5 0.349001840805542 2311 6 0.31602738149531007 2311 7 0.4329840014962514 2311 8 0.2626724176961872 2311 9 0.3985812901440653 2311 10-11 0.4851004432508432 2311 12-13 0.5540255303376327 2311 14-15 0.44186702876370987 2311 16-17 0.5871576030896364 2311 18-19 0.4261826863638518 2311 20-21 0.5117101060277918 2311 22-23 0.567345281839799 2311 24-25 0.5595557327471425 2311 26-27 0.7247182117397983 2311 28-29 0.6160384016648592 2311 30-31 0.5787772585320781 2311 32-33 0.6320887084827618 2311 34-35 0.546565508566367 2311 36-37 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0.3641629715296588 2312 62-63 0.3788242354598239 2312 64-65 0.37694422849695997 2312 66-67 0.37558116446719936 2312 68-69 0.5176331390490461 2312 70-71 0.47741537998427575 2312 72-73 0.5903074895802405 2312 74-75 0.4519568644701586 2312 76-77 0.5527293153002049 2312 78-79 0.7046710903536422 2312 80-81 0.8779682908115838 2312 82-83 0.7351352680627876 2312 84-85 0.7743638449047516 2312 86-87 0.876897575013647 2312 88-89 1.100712456762789 2312 90-91 0.8847279337493816 2312 92-93 1.0071623846814823 2312 94-95 0.906292959453527 2312 96-97 0.880783459390404 2312 98-99 1.0148466889511525 2312 100-101 0.9016832522652862 2313 1 0.13620159685647693 2313 2 0.20866535581968648 2313 3 0.2027812012662622 2313 4 0.06059609403520483 2313 5 0.2246993530815189 2313 6 0.3578155322974865 2313 7 0.35966857486469905 2313 8 0.3341158525300614 2313 9 0.38537689144170173 2313 10-11 0.4675050757130066 2313 12-13 0.3970070360472846 2313 14-15 0.4154757713162027 2313 16-17 0.28139987295144664 2313 18-19 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66-67 0.4307714295589875 2315 68-69 0.3958079429326915 2315 70-71 0.6387679794970005 2315 72-73 0.8774100915723118 2315 74-75 0.578338273995481 2315 76-77 0.7747697187395559 2315 78-79 0.7661126157801732 2315 80-81 0.7487992687652287 2315 82-83 0.7244550674670798 2315 84-85 0.5647532346217439 2315 86-87 0.6487745068382189 2315 88-89 0.7514203929873737 2315 90-91 0.9698654052782132 2315 92-93 1.1550744438949003 2315 94-95 1.2598742936066998 2315 96-97 1.2637161302422122 2315 98-99 1.428610866683968 2315 100-101 1.3571161627873067 2316 1 -0.06807489357061769 2316 2 -0.024640513094539074 2316 3 -0.027475845799187937 2316 4 0.07191227883848939 2316 5 -0.03159884720012229 2316 6 0.016855772947295122 2316 7 -0.01951496312528178 2316 8 -0.11870247621632046 2316 9 0.09064598999073326 2316 10-11 -0.5131569283334017 2316 12-13 -0.06149787453659172 2316 14-15 0.044771232089864554 2316 16-17 -0.1130992239786579 2316 18-19 -0.12121902362907377 2316 20-21 -0.04613761928785465 2316 22-23 0.04537353104364428 2316 24-25 0.2893942314439357 2316 26-27 0.2989705886760987 2316 28-29 0.2032500809112605 2316 30-31 0.40846171419792654 2316 32-33 0.27106931076706786 2316 34-35 0.2952381400771671 2316 36-37 0.002182608865389568 2316 38-39 0.18416353498589189 2316 40-41 0.14595458119112692 2316 42-43 0.08809573453644504 2316 44-45 0.14112445155103615 2316 46-47 0.12199048299304138 2316 48-49 0.3682301923018301 2316 50-51 0.32192520017567716 2316 52-53 0.24253472607966842 2316 54-55 0.27999313762234124 2316 56-57 0.28604044486733216 2316 58-59 0.5097678615909302 2316 60-61 0.318241632194038 2316 62-63 0.3606135234690413 2316 64-65 0.5757434391962093 2316 66-67 0.5962144800218496 2316 68-69 0.627154989536983 2316 70-71 0.6487647274822983 2316 72-73 0.6584015175807565 2316 74-75 0.6114384853764356 2316 76-77 0.6194389724021825 2316 78-79 0.7873173958763822 2316 80-81 0.6949450832578847 2316 82-83 0.6790118160370149 2316 84-85 0.704652897179546 2316 86-87 0.6639315444355276 2316 88-89 0.5844431406474762 2316 90-91 0.7775311164371921 2316 92-93 0.4969520804475458 2316 94-95 0.8687784194633181 2316 96-97 1.194927798032463 2316 98-99 0.6849500116233429 2316 100-101 0.34247067806355247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4575.0 3 1075.0 4 779.0 5 901.0 6 1080.0 7 1306.0 8 1664.0 9 2070.0 10 2637.0 11 3435.0 12 4687.0 13 6376.0 14 7145.0 15 9190.0 16 12093.0 17 15856.0 18 13549.0 19 8839.0 20 8851.0 21 9207.0 22 10084.0 23 11170.0 24 12392.0 25 14496.0 26 16711.0 27 19576.0 28 23530.0 29 28036.0 30 34094.0 31 41995.0 32 53404.0 33 68882.0 34 92293.0 35 130200.0 36 181756.0 37 233606.0 38 157759.0 39 15159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.134267496267825 19.035783509410162 14.845412153397211 21.984536840924797 2 15.128261398495308 20.686016796192312 40.04449512032606 24.141226684986318 3 18.97935105121054 25.742811984213553 29.791427490165272 25.48640947441063 4 12.433849392877361 16.535664082128523 37.092283277253735 33.93820324774038 5 13.633855494298421 38.139270840456 33.00315968075616 15.223713984489418 6 31.207171897766102 36.165767422328834 17.533753475558157 15.093307204346912 7 28.790499800766867 33.653935629803364 19.855390946305675 17.700173623124087 8 27.129381189969127 33.778881220983585 19.53990447546597 19.55183311358131 9 27.761586876225085 16.351997709859926 19.80732606266873 36.07908935124626 10-11 25.116269267535422 26.949220073388013 26.539891810730808 21.394618848345747 12-13 26.684051864514753 24.35295609998954 27.353374028693526 21.60961800680218 14-15 23.478564422431983 25.098831437283238 26.546066403434242 24.876537736850537 16-17 21.933145961972205 29.06506780680072 26.694096370594465 22.30768986063261 18-19 21.89204609839546 26.917184713654375 30.07333835641765 21.11743083153251 20-21 22.93891078181604 25.90391814483682 29.778369893991584 21.378801179355552 22-23 22.51992433404658 25.991962679096364 29.6755492262051 21.812563760651955 24-25 22.251672005130015 26.253472121453786 29.801347145356612 21.69350872805959 26-27 22.163818229599652 26.36111319838791 29.755820421041502 21.71924815097094 28-29 21.71106914682741 26.285878567875375 30.34184720520654 21.661205080090678 30-31 21.766183942170088 26.456335097937313 30.017023066832138 21.760457893060465 32-33 21.64130941781782 26.621075180223297 29.638654730381813 22.09896067157707 34-35 22.19548092382614 26.43097649792474 29.366797249216486 22.006745329032633 36-37 22.372400369099783 26.165691769877974 29.429107914773095 22.03279994624915 38-39 22.227001926238387 26.235883694163437 29.755512838607462 21.78160154099071 40-41 21.969779492386433 26.399372181835094 29.887396645752645 21.743451680025824 42-43 21.980197704893005 26.577408971781892 29.302967799801532 22.139425523523574 44-45 21.97778033154936 26.956945664444753 28.804250359571483 22.261023644434406 46-47 22.128766103517442 26.9834029780472 28.268860939098122 22.618969979337233 48-49 22.467348398021457 27.107212422919474 28.006687404407423 22.418751774651653 50-51 22.46121774375714 27.398717544914565 27.743629879199155 22.39643483212914 52-53 22.59485191867699 27.399300881773602 27.796830897397957 22.20901630215145 54-55 22.03278884105664 27.289701042919777 27.902687332849485 22.774822783174095 56-57 22.34900153341123 27.950027571187135 27.44845857781972 22.25251231758191 58-59 22.441199186402088 27.154088719060283 27.67318072343753 22.731531371100104 60-61 22.376067451995464 27.071743267758535 28.149688926482348 22.402500353763646 62-63 22.627990825286908 27.154964402272213 28.04941942510962 22.167625347331263 64-65 22.421284388207795 27.16228694127547 28.01625091372635 22.40017775679039 66-67 22.185489955091857 27.532929856960127 27.856047494993646 22.425532692954377 68-69 22.024528098637095 27.563722136781934 27.985787011070755 22.425962753510216 70-71 21.995198136495357 27.66080081409077 27.715537287980634 22.628463761433245 72-73 22.327581169431312 27.52837953707874 27.344688195774186 22.799351097715764 74-75 22.02918392423485 27.93406485943703 27.34385900889781 22.692892207430305 76-77 22.320742392020424 27.51265196974739 27.66464656584849 22.5019590723837 78-79 22.204469287068804 27.48011242789389 27.672488162869374 22.642930122167932 80-81 21.886647691133444 28.273756519526778 27.37811665182547 22.461479137514313 82-83 22.03443596146645 28.01119880300172 27.169698516393137 22.784666719138695 84-85 22.032455785025245 27.69140807765283 27.05140349824118 23.22473263908075 86-87 22.336609920721056 27.522141414045038 27.296453636805275 22.84479502842863 88-89 22.38713647617427 27.232981225323154 27.34933906204532 23.03054323645725 90-91 22.008754457736483 27.538931892545193 27.712231989154333 22.74008166056399 92-93 22.05335165149694 27.46198026981129 27.618646682059012 22.866021396632767 94-95 21.88036403272701 27.384939883081476 27.41276626869635 23.321929815495164 96-97 22.15308210450821 27.176611698682844 27.267414424195824 23.402891772613117 98-99 21.91224098543022 27.249020557361902 27.33573313676892 23.503005320438962 100-101 22.160703688289843 26.84971974816488 27.989329720924744 23.000246842620538 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2440.0 1 1484.0 2 563.0 3 636.0 4 613.0 5 623.0 6 1007.0 7 1961.0 8 2992.0 9 3327.0 10 2874.5 11 2492.5 12 2583.5 13 2594.5 14 2592.0 15 2709.0 16 2751.5 17 2789.0 18 2826.5 19 2905.5 20 3118.5 21 3455.0 22 3943.5 23 4578.0 24 5220.5 25 6001.5 26 7188.5 27 8460.0 28 9580.5 29 10926.0 30 12472.0 31 14119.5 32 16240.5 33 18750.5 34 21908.5 35 24119.5 36 26568.0 37 29619.0 38 31370.5 39 32014.5 40 32482.5 41 35027.0 42 37986.0 43 40251.0 44 42565.0 45 44947.0 46 45536.0 47 46799.0 48 55977.5 49 57262.0 50 50286.0 51 47207.0 52 44185.5 53 43367.5 54 42429.0 55 39396.0 56 35612.5 57 31652.5 58 28220.0 59 25366.0 60 22360.0 61 18839.0 62 15202.5 63 11618.5 64 8536.0 65 6600.0 66 5030.0 67 3897.5 68 2846.0 69 1873.5 70 1443.0 71 1144.5 72 911.5 73 731.5 74 518.0 75 386.0 76 288.0 77 179.0 78 100.0 79 65.5 80 42.0 81 24.0 82 15.5 83 11.0 84 9.0 85 8.5 86 6.0 87 6.0 88 4.0 89 1.5 90 2.0 91 2.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2496711512799316 2 0.2215067856287159 3 0.21182776419365026 4 0.2159532487397438 5 0.21706395611753826 6 0.24641836538781933 7 0.24887778886722128 8 0.23642199898766958 9 0.22951974599708996 10-11 0.24054748353376315 12-13 0.24328458385761367 14-15 0.22908339667009925 16-17 0.242927570771894 18-19 0.2324948550447536 20-21 0.22388687286684683 22-23 0.2241248815906599 24-25 0.2190870302699495 26-27 0.22519592084781884 28-29 0.24114250534329587 30-31 0.24169785903219304 32-33 0.24272923016871645 34-35 0.24939347443548296 36-37 0.22241915240333276 38-39 0.2241645497112954 40-41 0.21968205207948222 42-43 0.22420421783193095 44-45 0.24264989392744543 46-47 0.2478464177306979 48-49 0.25141654858789425 50-51 0.25244791972441766 52-53 0.2733926874199695 54-55 0.2515355529498008 56-57 0.22249848864460378 58-59 0.2053618605300613 60-61 0.20278343268875285 62-63 0.2104790480920427 64-65 0.20306110953320142 66-67 0.20286276893002383 68-69 0.20583787797768746 70-71 0.20730559844120153 72-73 0.20956668131742587 74-75 0.21234344976191194 76-77 0.22590994701925807 78-79 0.21920603463185603 80-81 0.21404917894923908 82-83 0.20984435816187452 84-85 0.2105980524539493 86-87 0.21793665477151955 88-89 0.21420785143178114 90-91 0.22317284669540755 92-93 0.21341448901907087 94-95 0.21095506553966892 96-97 0.22107043630172524 98-99 0.2265049688287908 100-101 0.20401314442845378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1260458.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.24688544792701 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03618270138077 35.237683403473866 2 14.00198677946935 13.51104504384892 3 5.16556616955473 7.476674377685826 4 2.4931512244353073 4.81147126118757 5 1.368206730624934 3.3005856700771954 6 0.8680129175077328 2.51273518789899 7 0.5667263992587871 1.9139948565748393 8 0.38731509340982656 1.4949397555197643 9 0.2930677445615189 1.2725645310307743 >10 1.5988662099818165 14.125348547554855 >50 0.1445778405874322 4.8095705787576675 >100 0.07173345962600688 6.0799371358336165 >500 0.00263241691533151 0.8474469836191952 >1k 0.0016452605720821936 1.4794601799076958 >5k 3.2905211441643876E-4 1.1265424870291887 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8190 0.6497638160097362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6002 0.4761761201087224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4407 0.3496348152814294 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2771 0.21984072456202428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2207 0.17509508448516334 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1858 0.14740673628157383 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1532 0.12154312162721805 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1362 0.10805596061114293 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1315 0.10432715727140451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.3800872381309014E-4 0.0 24-25 0.0 0.0 0.0 3.5701308571963527E-4 0.0 26-27 0.0 0.0 0.0 6.743580508037554E-4 0.0 28-29 0.0 0.0 0.0 0.001547056704785086 0.0 30-31 0.0 0.0 0.0 0.004085816425458048 0.0 32-33 0.0 0.0 0.0 0.0076559472826544 0.0 34-35 0.0 0.0 0.0 0.010670724450953541 0.0 36-37 0.0 0.0 0.0 0.013288820412897534 0.0 38-39 0.0 0.0 0.0 0.01547056704785086 0.0 40-41 0.0 0.0 0.0 0.01832667173360794 0.0 42-43 0.0 0.0 0.0 0.021103440178093992 0.0 44-45 0.0 0.0 0.0 0.02463390291465483 0.0 46-47 0.0 0.0 0.0 0.030822129733795178 0.0 48-49 0.0 0.0 0.0 0.03962845251487951 0.0 50-51 0.0 0.0 0.0 0.050616521930917174 0.0 52-53 0.0 0.0 0.0 0.076559472826544 0.0 54-55 0.0 0.0 0.0 0.1074609388016102 0.0 56-57 0.0 0.0 0.0 0.14514565340534946 0.0 58-59 0.0 0.0 0.0 0.20333878637765002 0.0 60-61 0.0 0.0 0.0 0.2892996037948111 0.0 62-63 0.0 0.0 0.0 0.35415698103387816 0.0 64-65 0.0 0.0 0.0 0.41885568579040316 0.0 66-67 0.0 7.933624127103005E-5 0.0 0.5353212879762752 0.0 68-69 0.0 7.933624127103005E-5 0.0 0.6808239544673444 0.0 70-71 0.0 7.933624127103005E-5 0.0 0.8113717394788245 0.0 72-73 0.0 7.933624127103005E-5 0.0 0.9341445728457434 0.0 74-75 0.0 7.933624127103005E-5 0.0 1.1423625380615618 0.0 76-77 0.0 7.933624127103005E-5 0.0 1.307302583664033 0.0 78-79 0.0 7.933624127103005E-5 0.0 1.4536779488090836 0.0 80-81 0.0 7.933624127103005E-5 0.0 1.628693697052976 0.0 82-83 0.0 7.933624127103005E-5 0.0 1.9018880438697678 0.0 84-85 0.0 7.933624127103005E-5 0.0 2.1917826694741116 0.0 86-87 0.0 7.933624127103005E-5 0.0 2.5440752488381206 0.0 88-89 0.0 7.933624127103005E-5 0.0 2.9503164722664303 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10730 0.0 28.60866 1 GTACATG 8675 0.0 26.621437 1 GGTATCA 7620 0.0 25.879618 1 TACATGG 8855 0.0 25.644863 2 ACATGGG 8915 0.0 24.832796 3 AGTACTT 6205 0.0 23.655165 12-13 TATCAAC 13215 0.0 23.187483 2 TCAACGC 13150 0.0 23.124222 4 ATCAACG 13245 0.0 22.99149 3 GAGTACT 6220 0.0 22.910793 12-13 CAACGCA 13395 0.0 22.768589 5 CATGGGG 3060 0.0 22.514355 4 AACGCAG 13575 0.0 22.46579 6 CATGGGA 6050 0.0 22.146578 4 AGGACCG 195 4.2173997E-7 21.92561 5 GTACTTT 6535 0.0 21.768372 14-15 ATGGGAG 2135 0.0 21.138275 5 ATTCGCG 135 1.0059011E-9 21.105995 80-81 ACGCAGA 14735 0.0 20.47152 7 TACTTTT 7130 0.0 20.351498 14-15 >>END_MODULE