FastQCFastQC Report
Thu 26 May 2016
SRR937276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260458
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117310.9306934463504536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.7304487733823737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67580.536154318509621No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27480.2180159910127906No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25290.20064135417443502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.16144925098654617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.13495094640202213No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13440.1066279082682644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127150.027.927571
GGTATCA90700.027.2536661
GTACATG81000.024.707341
GAGTACT82450.023.93671612-13
TACATGG83250.023.8483872
ACATGGG82100.023.0253853
TCAACGC153650.022.9989154
ATCAACG154050.022.9391963
TATCAAC154650.022.8501972
AGTACTT83050.022.8486912-13
CAACGCA155600.022.7717385
GTACTTT86750.022.74932314-15
AACGCAG158750.022.289976
TATAGGG1503.034952E-522.1653062
CATGGGG29000.021.1280144
ATTCGCG1501.6734703E-1020.58370480-81
ACGCAGA173350.020.3860597
CGCAGAG174450.020.2847428
ACTTTTT98200.020.12096416-17
TACTTTT94250.019.93113714-15
Produced by FastQC (version 0.11.5)