##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1260458 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19087823632362 33.0 31.0 34.0 31.0 34.0 2 32.386859379685795 34.0 31.0 34.0 31.0 34.0 3 32.43241662951086 34.0 31.0 34.0 31.0 34.0 4 36.09117241510626 37.0 35.0 37.0 35.0 37.0 5 36.02025850920856 37.0 35.0 37.0 35.0 37.0 6 36.07032681771229 37.0 35.0 37.0 35.0 37.0 7 36.01311666076934 37.0 35.0 37.0 35.0 37.0 8 35.99442424896347 37.0 35.0 37.0 35.0 37.0 9 37.69099486059829 39.0 37.0 39.0 35.0 39.0 10-11 37.61752394764443 39.0 37.0 39.0 35.0 39.0 12-13 37.51063740322962 39.0 37.0 39.0 35.0 39.0 14-15 38.67109455451907 40.0 38.0 41.0 34.0 41.0 16-17 38.652168894163864 40.0 38.0 41.0 34.5 41.0 18-19 38.68446350453565 40.0 38.0 41.0 34.5 41.0 20-21 38.617418827124744 40.0 38.0 41.0 34.0 41.0 22-23 38.47306812285693 40.0 38.0 41.0 34.0 41.0 24-25 38.39826713781816 40.0 38.0 41.0 34.0 41.0 26-27 38.335099622518165 40.0 38.0 41.0 34.0 41.0 28-29 38.19569513621239 40.0 38.0 41.0 34.0 41.0 30-31 38.20162948705946 40.0 37.5 41.0 34.0 41.0 32-33 38.09845667209855 40.0 37.0 41.0 34.0 41.0 34-35 37.947782869401436 40.0 37.0 41.0 33.0 41.0 36-37 37.75953740624439 40.0 37.0 41.0 33.0 41.0 38-39 37.62131145980271 40.0 37.0 41.0 33.0 41.0 40-41 37.4059972644864 40.0 36.0 41.0 33.0 41.0 42-43 37.15053774104334 40.0 36.0 41.0 32.5 41.0 44-45 36.78447120015106 40.0 36.0 41.0 31.0 41.0 46-47 36.31445672922065 39.0 35.0 41.0 30.5 41.0 48-49 35.90517494434563 39.0 35.0 41.0 29.0 41.0 50-51 35.62797173725741 39.0 35.0 41.0 28.0 41.0 52-53 35.359041713408935 39.0 35.0 40.0 27.5 41.0 54-55 35.11148090614681 38.5 35.0 40.0 27.0 41.0 56-57 34.852671806597286 38.0 34.5 40.0 26.0 41.0 58-59 34.57726318528662 38.0 34.0 40.0 26.0 41.0 60-61 34.23578056547699 37.5 34.0 40.0 25.5 41.0 62-63 33.86276932670505 37.0 33.5 40.0 24.5 41.0 64-65 33.44495849921219 36.0 33.0 39.0 23.0 41.0 66-67 33.02789343238727 36.0 33.0 39.0 22.0 40.0 68-69 32.6085153174481 35.0 32.0 38.5 21.5 40.0 70-71 32.20539954524466 35.0 32.0 38.0 20.0 40.0 72-73 31.739673198154957 35.0 31.0 37.0 18.5 39.0 74-75 31.281222381071007 35.0 31.0 36.5 18.0 39.0 76-77 30.01175604423154 33.5 29.5 35.0 16.0 37.5 78-79 30.609868793724186 34.0 31.0 36.0 15.5 37.5 80-81 30.449878139533407 34.0 31.0 35.0 11.5 37.0 82-83 30.132949293034756 34.0 30.5 35.0 7.5 37.0 84-85 29.800617711974535 34.0 30.0 35.0 4.5 36.0 86-87 29.400976867138773 34.0 30.0 35.0 2.0 36.0 88-89 28.9440227282464 34.0 29.0 35.0 2.0 35.5 90-91 28.45190438713547 34.0 29.0 35.0 2.0 35.0 92-93 28.13943701416469 33.5 29.0 35.0 2.0 35.0 94-95 27.72743954975096 33.0 27.5 35.0 2.0 35.0 96-97 27.25847509397378 33.0 27.0 35.0 2.0 35.0 98-99 26.530014883478863 33.0 25.5 35.0 2.0 35.0 100-101 24.089580136743947 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03421107232495757 1101 2 -0.006211433118274101 1101 3 0.025693290490551135 1101 4 0.019276657214774673 1101 5 0.08800430726435593 1101 6 -0.06750700871612736 1101 7 0.019383073582574184 1101 8 -0.001994164879000948 1101 9 0.068950511173135 1101 10-11 -0.05294352846213002 1101 12-13 -0.06683979319912225 1101 14-15 -0.18733792626230183 1101 16-17 -0.03299893956888411 1101 18-19 0.15289607773610214 1101 20-21 -0.01152503382208181 1101 22-23 -0.09409171517838644 1101 24-25 -0.05210659472755452 1101 26-27 -0.11386669677059302 1101 28-29 -0.24432965311366672 1101 30-31 -5.622742581863349E-4 1101 32-33 0.07756929961424675 1101 34-35 0.00826691992322992 1101 36-37 -0.11189865648986341 1101 38-39 0.03781854302151544 1101 40-41 0.07326178687127793 1101 42-43 0.09346435132290054 1101 44-45 0.24687174718817317 1101 46-47 0.03147541592959868 1101 48-49 -0.03692837955986761 1101 50-51 0.14946838130740758 1101 52-53 -0.1085813305215737 1101 54-55 0.057427658857236 1101 56-57 0.14421008911436672 1101 58-59 0.16509750650352117 1101 60-61 0.23412367608118956 1101 62-63 0.1712714243707225 1101 64-65 0.22137695273328006 1101 66-67 0.16376750873139656 1101 68-69 0.29776206386569015 1101 70-71 0.23588738667143616 1101 72-73 0.08797116093301582 1101 74-75 0.09116020534121816 1101 76-77 0.14633359414436597 1101 78-79 -0.0806673414754151 1101 80-81 -0.01274204315927463 1101 82-83 -0.07995103108119395 1101 84-85 -0.1086641353050446 1101 86-87 -0.160139291733163 1101 88-89 -0.016580223943375216 1101 90-91 -0.0563540417497137 1101 92-93 -0.2016956833755401 1101 94-95 -0.15496651447609366 1101 96-97 -0.24415085477351894 1101 98-99 0.06037320268470481 1101 100-101 0.6786632150180836 1102 1 -0.005562447529328551 1102 2 -0.03706311079555036 1102 3 -0.0656177773365485 1102 4 -0.10844846166573774 1102 5 -0.04420958772085015 1102 6 -0.08246587184252974 1102 7 -0.04114558090734732 1102 8 -0.08353449208597397 1102 9 -0.16102553118535923 1102 10-11 -0.09284956356463425 1102 12-13 -0.15704737954354187 1102 14-15 -0.06284393298876267 1102 16-17 -0.2747960028725842 1102 18-19 -0.14353295452377068 1102 20-21 0.0028283249119027687 1102 22-23 -0.036686658993268395 1102 24-25 -0.265134379223845 1102 26-27 -0.19507131536146716 1102 28-29 -0.30574589660687224 1102 30-31 -0.10125921875118848 1102 32-33 -0.05982433912642193 1102 34-35 -0.102539825306323 1102 36-37 -0.2747681663832182 1102 38-39 -0.07266143092614641 1102 40-41 -0.022922083139668814 1102 42-43 -0.09206884165704565 1102 44-45 -0.04108022357780072 1102 46-47 0.015106409040591018 1102 48-49 0.0955991599976116 1102 50-51 0.0817669575342066 1102 52-53 0.036841385835927554 1102 54-55 -0.00967999077468562 1102 56-57 0.044336775826721464 1102 58-59 0.037903879716544964 1102 60-61 0.008927431772455918 1102 62-63 0.14399719400088173 1102 64-65 0.05780977218851291 1102 66-67 0.13709068329484353 1102 68-69 0.05712797117547552 1102 70-71 0.2510990087808409 1102 72-73 0.22456099817856767 1102 74-75 0.1152354286488908 1102 76-77 0.09641132102278505 1102 78-79 0.04425424738151662 1102 80-81 -0.1269279385935782 1102 82-83 0.10968328113228765 1102 84-85 0.1215696932869399 1102 86-87 -0.02071185087736538 1102 88-89 -0.01376105358493973 1102 90-91 0.04030183122394959 1102 92-93 0.17961121183365947 1102 94-95 0.2897342887109602 1102 96-97 0.15894581981200062 1102 98-99 0.3050533941447462 1102 100-101 0.9684702412150834 1103 1 -0.04993888560705528 1103 2 0.03492898727064642 1103 3 -0.0717891647996538 1103 4 -0.043809615439421634 1103 5 -0.026243536346861163 1103 6 -0.10796502398330432 1103 7 -0.043165598378379855 1103 8 -0.20755260258744102 1103 9 -0.10656898896654354 1103 10-11 -0.12385066549615686 1103 12-13 -0.16955687157023647 1103 14-15 -0.09210678076806289 1103 16-17 -0.14433387677279086 1103 18-19 -0.017501963413444344 1103 20-21 -0.14338656886303625 1103 22-23 -0.2720435306496469 1103 24-25 -0.0609715902523007 1103 26-27 -0.06522039013865566 1103 28-29 -0.16772354966401792 1103 30-31 -0.10144877381065953 1103 32-33 -0.2007374007261049 1103 34-35 -0.020859008620909947 1103 36-37 -0.020272514159657362 1103 38-39 -0.012807422578873684 1103 40-41 -0.007210423790660059 1103 42-43 0.08881607215820253 1103 44-45 0.04123748668842353 1103 46-47 0.013851177720617613 1103 48-49 -0.18235193251324944 1103 50-51 -0.10930539882930645 1103 52-53 -0.11985042161723669 1103 54-55 -0.11965818052387078 1103 56-57 -0.2671975353518832 1103 58-59 -0.25710802456956827 1103 60-61 -0.1927976905533555 1103 62-63 -0.037508626823914426 1103 64-65 -0.1083903340079928 1103 66-67 -0.11040827472327663 1103 68-69 -0.2429271254005414 1103 70-71 -0.06781481789047206 1103 72-73 -0.24561748047079135 1103 74-75 -0.060760163625026564 1103 76-77 -0.06726804545223075 1103 78-79 -0.1018181946243395 1103 80-81 -0.19588315906552722 1103 82-83 -0.31575592136618624 1103 84-85 -0.2634899994283266 1103 86-87 -0.16433006871723776 1103 88-89 -0.010355913902273528 1103 90-91 -0.22385855976190427 1103 92-93 -0.2099352833960637 1103 94-95 -0.2912702389144073 1103 96-97 -0.3314533877108232 1103 98-99 -0.2732202845365883 1103 100-101 0.4674753626273862 1104 1 0.174123010833668 1104 2 0.1700929254661787 1104 3 0.1047927353199114 1104 4 0.1030668216064683 1104 5 0.046035273549208 1104 6 0.11321588285013462 1104 7 0.041523539853933755 1104 8 0.08979288623809367 1104 9 0.15206133385144938 1104 10-11 0.0542108170753508 1104 12-13 0.1330160153974802 1104 14-15 0.1682468474854133 1104 16-17 0.0880065536511978 1104 18-19 0.08227040851702583 1104 20-21 0.10560405010136265 1104 22-23 -0.0401549074545855 1104 24-25 0.08512999164781831 1104 26-27 -0.1685641085236469 1104 28-29 -0.05466425140226505 1104 30-31 0.050661384379345975 1104 32-33 0.04795776160259635 1104 34-35 0.14469959985881076 1104 36-37 -0.0634505698940444 1104 38-39 -0.04946646332200544 1104 40-41 0.034829491596077844 1104 42-43 -0.009373250015990209 1104 44-45 0.1030804693059224 1104 46-47 -0.09135552696070448 1104 48-49 0.1474023901207957 1104 50-51 0.09499366766568329 1104 52-53 0.14508278448963097 1104 54-55 0.2042830040618071 1104 56-57 0.257411588165084 1104 58-59 0.05849484492367196 1104 60-61 0.18029793622857682 1104 62-63 0.13376310131000224 1104 64-65 0.15451606257068562 1104 66-67 0.35151748173768027 1104 68-69 0.3883096537772417 1104 70-71 0.46940294629604296 1104 72-73 0.6556770104700718 1104 74-75 0.46976499042629527 1104 76-77 0.547987633753138 1104 78-79 0.5444161703163388 1104 80-81 0.46021278961469037 1104 82-83 0.5004148686641656 1104 84-85 0.6227172340858864 1104 86-87 0.45791770098753304 1104 88-89 0.5626598131279863 1104 90-91 0.5837129476417573 1104 92-93 0.8050364355407567 1104 94-95 0.8396549887136615 1104 96-97 0.8764601366154885 1104 98-99 1.268773645381554 1104 100-101 1.686265599912403 1105 1 7.897228283724189E-4 1105 2 0.01918851776765962 1105 3 0.03516320138280804 1105 4 0.045730818451637845 1105 5 -0.00443353169433891 1105 6 -0.021607836406118963 1105 7 -0.05332878139494568 1105 8 0.04082217268410204 1105 9 0.0415691393397708 1105 10-11 0.06950053123674138 1105 12-13 0.05690910468464949 1105 14-15 0.031806050437168665 1105 16-17 0.07127994065292853 1105 18-19 0.0926040682122391 1105 20-21 0.1031869324207122 1105 22-23 0.06561693839382343 1105 24-25 -0.06082799379281312 1105 26-27 0.14169923605103918 1105 28-29 -0.11420744675705663 1105 30-31 0.020010281094883453 1105 32-33 0.09715058254777631 1105 34-35 0.0938627875984821 1105 36-37 0.1828472625191253 1105 38-39 0.11113786142620086 1105 40-41 -0.024171750392191882 1105 42-43 0.20912319626575737 1105 44-45 0.2634564540575681 1105 46-47 0.13311062205975333 1105 48-49 0.23203068886883926 1105 50-51 0.21298680273417148 1105 52-53 0.281298924444485 1105 54-55 0.28194636631442904 1105 56-57 0.405569683299575 1105 58-59 0.38835605046819666 1105 60-61 0.36547289112717607 1105 62-63 0.3828378427135348 1105 64-65 0.5141184110163906 1105 66-67 0.5514013852977442 1105 68-69 0.5813294757287011 1105 70-71 0.7966019794489725 1105 72-73 0.7972285439176261 1105 74-75 0.746120978713531 1105 76-77 0.8192226868322727 1105 78-79 0.742583840999071 1105 80-81 0.6728616355628176 1105 82-83 0.6828069652672824 1105 84-85 0.6013532793957914 1105 86-87 0.6149340281471858 1105 88-89 0.8693085298912031 1105 90-91 1.068284416360875 1105 92-93 1.021808149937847 1105 94-95 1.0649920026837663 1105 96-97 1.0351578507224808 1105 98-99 1.1963303734223878 1105 100-101 1.4509431022467965 1106 1 -0.148182339600325 1106 2 -0.06389255562871199 1106 3 -0.10336477953212864 1106 4 -0.11261040342267847 1106 5 -0.05312056640045171 1106 6 -0.07979579519846425 1106 7 -0.0671345949895894 1106 8 -0.047584966185425515 1106 9 -0.03538314452287494 1106 10-11 -0.12422296413242151 1106 12-13 -0.10177799937127219 1106 14-15 -0.15658011011457518 1106 16-17 0.03383711090324226 1106 18-19 -0.0034202805063472397 1106 20-21 -0.023412014155667293 1106 22-23 -0.00623813437146481 1106 24-25 -0.17490669889314603 1106 26-27 -0.024815727904403673 1106 28-29 -0.2455585891218064 1106 30-31 -0.1581571848283545 1106 32-33 -0.20496753405006274 1106 34-35 -0.16051145172077952 1106 36-37 -0.14376954163353872 1106 38-39 -0.4630777055258477 1106 40-41 -0.3340423089702469 1106 42-43 -0.2973350048695025 1106 44-45 -0.24033871434110665 1106 46-47 -0.32845484589542906 1106 48-49 -0.3174564692799393 1106 50-51 -0.0936659246205167 1106 52-53 0.12909776144815766 1106 54-55 0.09861494010089444 1106 56-57 -0.023275677024706454 1106 58-59 -0.04137005245385694 1106 60-61 -0.03286288678698668 1106 62-63 0.09495464166496248 1106 64-65 0.009969558582383797 1106 66-67 0.09323145745622696 1106 68-69 0.19314304263220095 1106 70-71 0.12068144739722442 1106 72-73 0.15708124491938946 1106 74-75 0.15739569758673255 1106 76-77 0.3239046659371745 1106 78-79 0.4046422127348599 1106 80-81 0.1615428795597218 1106 82-83 0.12109343546593365 1106 84-85 -0.03994782708043587 1106 86-87 0.020097294939379395 1106 88-89 0.21477981386827238 1106 90-91 0.0841871948610482 1106 92-93 0.07360032242469572 1106 94-95 0.4327462477590629 1106 96-97 0.44624445823376035 1106 98-99 0.43367750043309883 1106 100-101 1.1961224235201975 1107 1 0.01596366722778697 1107 2 -0.03320021743182622 1107 3 0.04266142074409629 1107 4 0.024438510141024494 1107 5 -0.03665081203943288 1107 6 -0.03857525797428707 1107 7 0.021364267750684007 1107 8 0.011423771798135363 1107 9 0.026777557797679208 1107 10-11 -0.012628498644495778 1107 12-13 0.02365274629734415 1107 14-15 0.016840033284992728 1107 16-17 0.050103457587411526 1107 18-19 0.11503648767043728 1107 20-21 0.19369975636857362 1107 22-23 0.16453030222687204 1107 24-25 0.17410383956173092 1107 26-27 -0.01329726965482081 1107 28-29 0.15701369182464475 1107 30-31 0.0716507573013061 1107 32-33 0.014608489141835435 1107 34-35 0.05809539371674077 1107 36-37 -0.052251470827151536 1107 38-39 -0.14341086109112666 1107 40-41 -0.1319012295079247 1107 42-43 -0.22180979143388413 1107 44-45 -0.08364529130327725 1107 46-47 -0.0436476330705986 1107 48-49 0.19259540252649998 1107 50-51 0.06031824490639082 1107 52-53 0.16743070733829768 1107 54-55 0.29426235767805053 1107 56-57 0.20885236611367475 1107 58-59 0.3283100106310073 1107 60-61 0.3167595840066326 1107 62-63 0.3480855245340493 1107 64-65 0.28933091545403045 1107 66-67 0.1927000905110532 1107 68-69 0.2106144516035613 1107 70-71 0.30245213116609193 1107 72-73 0.4149053183603826 1107 74-75 0.41415829063007337 1107 76-77 0.4752337100464423 1107 78-79 0.2705264720574263 1107 80-81 0.402873268324786 1107 82-83 0.219986031654404 1107 84-85 0.34092649546374076 1107 86-87 0.44418919646499333 1107 88-89 0.7167569136964325 1107 90-91 0.5754856765395431 1107 92-93 0.6954608930145696 1107 94-95 0.8685554570558764 1107 96-97 0.7860931046895665 1107 98-99 1.1656266359789598 1107 100-101 1.7164050747256034 1108 1 -0.021204710493989865 1108 2 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0.1994296756273748 2316 86-87 -0.15474559009051703 2316 88-89 -0.06619059351362822 2316 90-91 0.10727912811087492 2316 92-93 0.14991066666787134 2316 94-95 0.2157561838653841 2316 96-97 0.19043632723554182 2316 98-99 0.24291897293046816 2316 100-101 -0.022729986243657407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 14.0 5 34.0 6 76.0 7 157.0 8 343.0 9 617.0 10 1150.0 11 1922.0 12 3024.0 13 4511.0 14 5620.0 15 8082.0 16 11685.0 17 17199.0 18 14629.0 19 7926.0 20 6456.0 21 6510.0 22 6700.0 23 7236.0 24 8471.0 25 9794.0 26 11790.0 27 14942.0 28 18661.0 29 23107.0 30 29057.0 31 36269.0 32 46558.0 33 61371.0 34 85644.0 35 124538.0 36 187967.0 37 276373.0 38 206001.0 39 16019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09390990905157 19.28757726249598 15.386600291384314 21.231912537068133 2 15.96737059069005 21.265286110286898 39.16473218465034 23.602611114372714 3 19.182630440681088 25.732471847534782 30.396728808099915 24.688168903684215 4 12.908958489691841 17.307915059446646 36.75092704397925 33.03219940688226 5 13.541028659924025 38.03937685055013 33.190261147729835 15.229333341796014 6 31.235471550817245 36.710148215965944 16.86490148818921 15.189478745027602 7 28.462669918394738 34.1080781747587 19.77677955155983 17.65247235528673 8 27.40829127190275 33.82778323434815 19.121144853696038 19.642780640053058 9 27.60441046032474 16.247744867341872 19.773447429426447 36.374397242906944 10-11 25.295012281269635 27.07349562380599 26.191949580151945 21.43954251477243 12-13 26.9488839944525 24.43684008416456 27.413463155790126 21.200812765592815 14-15 23.60050870397903 25.0836600664203 26.801845043626997 24.513986185973668 16-17 21.74566705118298 28.93940139219236 27.132042479797025 22.18288907682763 18-19 21.672400032369186 26.25204489161876 31.1606971434193 20.914857932592756 20-21 22.527724049512162 25.25772377976894 31.224404145159934 20.990148025558963 22-23 22.33474830586097 25.19916521777764 31.107776319302218 21.358310157059172 24-25 22.13618999639016 25.418086469461183 31.260626207410873 21.18509732673778 26-27 22.084264875989128 25.64008682585798 31.160002665706166 21.11564563244672 28-29 21.539721799634645 25.566982499915703 31.69183349234086 21.20146220810879 30-31 21.513112928709447 25.709297717609005 31.44215043065815 21.3354389230234 32-33 21.60135442831098 25.792331041573778 31.05728235292251 21.549032177192736 34-35 22.161825304770172 25.478794216070668 30.885995408018356 21.473385071140807 36-37 22.28602619047997 25.301200039985467 30.873301609414987 21.539472160119573 38-39 22.108616591508444 25.403625048792023 31.17516573419694 21.312592625502596 40-41 21.916773043427902 25.539863716096622 31.305946970027538 21.23741627044794 42-43 21.996433452548853 25.727660975662975 30.688296051209115 21.587609520579058 44-45 22.022673549363702 26.00321867769231 30.058260684331973 21.915847088612015 46-47 22.089192975886544 26.22134176624687 29.44425756352056 22.24520769434602 48-49 22.499123334533955 26.356649725734616 29.074868024162647 22.069358915568785 50-51 22.577507540909732 26.641982517465873 28.657004041388127 22.12350590023626 52-53 22.705556234321175 26.800655000007932 28.572748953158296 21.921039812512593 54-55 22.057101466292412 26.70886296885735 28.67560838996619 22.55842717488405 56-57 22.451799266615787 27.192695036248725 28.255880005521806 22.099625691613685 58-59 22.638001424878894 26.460143852472672 28.488176519963375 22.413678202685055 60-61 22.479646287301914 26.45161520653604 28.96609803738006 22.10264046878198 62-63 22.74153521973759 26.44594266528516 28.854630618394268 21.957891496582988 64-65 22.595715208281433 26.418730334529194 28.8813669317026 22.10418752548677 66-67 22.252070279215967 26.94139749202274 28.70892961130002 22.097602617461273 68-69 22.252823973508043 26.766580084382024 28.81250307427935 22.168092867830584 70-71 22.138302109233308 27.035093592963825 28.556683364300913 22.26992093350195 72-73 22.467547510508084 26.92779132664476 28.146872010015407 22.457789152831747 74-75 22.28324942203548 27.29051662173591 27.920882726754876 22.50535122947373 76-77 22.473656401085954 26.89930961602846 28.483892362934743 22.143141619950843 78-79 22.28646253980696 26.81366614357638 28.45794941203912 22.44192190457754 80-81 21.93489268777408 27.647501006578963 28.095988956390833 22.321617349256123 82-83 22.154614253764322 27.380195850222105 27.941700325200543 22.523489570813034 84-85 22.114628629548267 27.00739337365217 27.817379941973243 23.060598054826322 86-87 22.33644437180771 26.80720023991279 28.04012509738524 22.816230290894264 88-89 22.408640351364344 26.55348294032804 28.09324071091619 22.944635997391423 90-91 22.018724585936724 26.897748685906976 28.412575622334625 22.67095110582168 92-93 21.895693574536637 26.7958050840566 28.30501166069847 23.0034896807083 94-95 21.788191276504254 26.710053012476415 28.13993008890419 23.361825622115138 96-97 22.046827423047812 26.602790414277983 28.042981202070994 23.307400960603207 98-99 21.91044352202695 26.508581021471596 27.95857213579847 23.622403320702983 100-101 21.598625152536023 26.64175575511085 28.523483010382765 23.23613608197036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 971.0 1 683.0 2 548.0 3 725.0 4 564.0 5 481.0 6 1022.5 7 2493.5 8 4092.5 9 4675.0 10 3863.0 11 2815.5 12 2650.5 13 2803.0 14 2930.5 15 2968.0 16 2970.0 17 3004.5 18 3088.0 19 3162.0 20 3337.0 21 3620.0 22 4061.0 23 4634.0 24 5258.5 25 6065.5 26 7207.0 27 8569.0 28 9720.5 29 10899.0 30 12364.0 31 13926.0 32 15951.5 33 18361.5 34 21366.5 35 23610.0 36 26156.0 37 29527.5 38 31624.0 39 32114.5 40 32460.5 41 35017.0 42 37814.5 43 40180.5 44 42596.5 45 44928.0 46 45566.5 47 46613.0 48 56841.5 49 58093.5 50 50302.5 51 47339.0 52 43641.5 53 43101.5 54 42458.0 55 39214.5 56 35279.5 57 31178.5 58 27794.0 59 25018.5 60 22200.5 61 18462.5 62 14932.0 63 11681.0 64 8470.0 65 6502.0 66 4920.0 67 3739.5 68 2656.5 69 1682.0 70 1244.5 71 990.5 72 827.0 73 673.0 74 510.0 75 399.0 76 281.5 77 162.0 78 103.0 79 71.0 80 40.5 81 26.0 82 14.5 83 11.0 84 8.0 85 4.0 86 2.5 87 1.5 88 1.0 89 1.0 90 1.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07560743793129164 2 0.0 3 0.0 4 0.0 5 0.0017453973079626611 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.586724825420601E-4 12-13 0.005553536888972104 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.536942920747855E-4 24-25 0.0010313711365233908 26-27 3.173449650841202E-4 28-29 0.002816436565121567 30-31 2.776768444486052E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.7601744762618034E-4 40-41 7.933624127103005E-5 42-43 0.010750060692224572 44-45 1.983406031775751E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.9668120635515026E-5 82-83 3.966812063551502E-4 84-85 3.966812063551502E-4 86-87 0.0 88-89 0.0 90-91 0.006584908025495494 92-93 0.0021817466349533265 94-95 0.0 96-97 0.0 98-99 1.586724825420601E-4 100-101 0.007933624127103005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1260458.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.73160239846392 #Duplication Level Percentage of deduplicated Percentage of total 1 71.077404295937 32.50483592776662 2 14.858275568165698 13.589855012203286 3 5.611068848376422 7.698095088130722 4 2.7117437821994295 4.960495538162042 5 1.4585359193562706 3.33505923739395 6 0.9366536093622443 2.570080226906418 7 0.6335000009177534 2.0279679112978064 8 0.4198900249262496 1.5361794936806683 9 0.32178920021029467 1.3244342184123208 >10 1.7288073843289267 14.450762739764167 >50 0.15678977172529482 4.963070535465248 >100 0.07912164240068896 6.380847785298624 >500 0.004511317687411844 1.3810853226151214 >1k 0.0013880977499728753 1.0795556342533024 >5k 3.470244374932188E-4 1.26682144070959 >10k+ 1.735122187466094E-4 0.9308538879401316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11731 0.9306934463504536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9207 0.7304487733823737 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6758 0.536154318509621 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2748 0.2180159910127906 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2529 0.20064135417443502 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2035 0.16144925098654617 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1701 0.13495094640202213 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1344 0.1066279082682644 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.3800872381309014E-4 0.0 24-25 0.0 0.0 0.0 3.9668120635515027E-4 0.0 26-27 0.0 0.0 0.0 6.743580508037554E-4 0.0 28-29 0.0 0.0 0.0 0.001586724825420601 0.0 30-31 0.0 0.0 0.0 0.004284157028635623 0.0 32-33 0.0 0.0 0.0 0.007933624127103005 0.0 34-35 0.0 0.0 0.0 0.011067405657308692 0.0 36-37 0.0 0.0 0.0 0.013844174101794744 0.0 38-39 0.0 0.0 0.0 0.015986252616112556 0.0 40-41 0.0 0.0 0.0 0.018842357301869637 0.0 42-43 0.0 0.0 0.0 0.021658793866991204 0.0 44-45 0.0 0.0 0.0 0.02503058412100998 7.933624127103005E-5 46-47 0.0 0.0 0.0 0.031218810940150325 7.933624127103005E-5 48-49 0.0 0.0 0.0 0.04034247868631878 7.933624127103005E-5 50-51 0.0 0.0 0.0 0.051330548102356446 7.933624127103005E-5 52-53 0.0 0.0 0.0 0.0781065295313291 7.933624127103005E-5 54-55 0.0 0.0 0.0 0.10972202167783457 7.933624127103005E-5 56-57 0.0 0.0 0.0 0.1475257406434804 7.933624127103005E-5 58-59 0.0 0.0 0.0 0.20595688233959403 7.933624127103005E-5 60-61 0.0 0.0 0.0 0.2920367041186616 7.933624127103005E-5 62-63 0.0 0.0 0.0 0.3569734175989997 7.933624127103005E-5 64-65 0.0 0.0 0.0 0.42194979919997333 7.933624127103005E-5 66-67 0.0 0.0 0.0 0.5390500913160137 7.933624127103005E-5 68-69 0.0 7.933624127103005E-5 0.0 0.6860204782705969 7.933624127103005E-5 70-71 0.0 7.933624127103005E-5 0.0 0.8175202981773292 7.933624127103005E-5 72-73 0.0 7.933624127103005E-5 0.0 0.9403327996648837 7.933624127103005E-5 74-75 0.0 7.933624127103005E-5 0.0 1.1504548346712067 7.933624127103005E-5 76-77 0.0 7.933624127103005E-5 0.0 1.3175369587879961 7.933624127103005E-5 78-79 0.0 7.933624127103005E-5 0.0 1.4652610400346542 7.933624127103005E-5 80-81 0.0 7.933624127103005E-5 0.0 1.6419031812246025 7.933624127103005E-5 82-83 0.0 7.933624127103005E-5 0.0 1.9164859122636375 7.933624127103005E-5 84-85 0.0 7.933624127103005E-5 0.0 2.2070945640394206 7.933624127103005E-5 86-87 0.0 7.933624127103005E-5 0.0 2.5630762786225327 7.933624127103005E-5 88-89 0.0 7.933624127103005E-5 0.0 2.9759817463176086 7.933624127103005E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12715 0.0 27.92757 1 GGTATCA 9070 0.0 27.253666 1 GTACATG 8100 0.0 24.70734 1 GAGTACT 8245 0.0 23.936716 12-13 TACATGG 8325 0.0 23.848387 2 ACATGGG 8210 0.0 23.025385 3 TCAACGC 15365 0.0 22.998915 4 ATCAACG 15405 0.0 22.939196 3 TATCAAC 15465 0.0 22.850197 2 AGTACTT 8305 0.0 22.84869 12-13 CAACGCA 15560 0.0 22.771738 5 GTACTTT 8675 0.0 22.749323 14-15 AACGCAG 15875 0.0 22.28997 6 TATAGGG 150 3.034952E-5 22.165306 2 CATGGGG 2900 0.0 21.128014 4 ATTCGCG 150 1.6734703E-10 20.583704 80-81 ACGCAGA 17335 0.0 20.386059 7 CGCAGAG 17445 0.0 20.284742 8 ACTTTTT 9820 0.0 20.120964 16-17 TACTTTT 9425 0.0 19.931137 14-15 >>END_MODULE