FastQCFastQC Report
Thu 26 May 2016
SRR937275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444802
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.5323717069617493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.4368235754335637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.2785509057962869No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6100.13713967113457223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.043.0685771
GTATCAA21900.036.4520231
GTACTAG550.002573188934.558411
TGGACCG550.00257603634.550625
TATCAAC27900.028.092432
AGGGCCG856.395337E-427.9453545
ATCAACG28150.027.8429413
TCAACGC28800.027.217614
CGCTCCG350.00838905227.14385448-49
CAACGCA29050.026.983385
AACGCAG29150.026.884756
GTCTAGG950.001222691625.0093771
GTACATG20650.024.1615981
GGACCGA1000.001652781923.7481966
CGCAGAG33250.023.2839478
TACATGG20950.023.1273422
ACGCAGA33500.023.1101867
TAAGGTG2503.8016879E-1022.8034085
ACATGGG21150.022.6840523
GAGTACT18650.022.15649412-13
Produced by FastQC (version 0.11.5)