##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937275_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 444802 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79781340911237 33.0 31.0 34.0 30.0 34.0 2 31.92372336455322 33.0 31.0 34.0 30.0 34.0 3 31.948341059617537 33.0 31.0 34.0 30.0 34.0 4 35.57014356949834 37.0 35.0 37.0 33.0 37.0 5 35.583790090871894 37.0 35.0 37.0 33.0 37.0 6 35.63641350533496 37.0 35.0 37.0 33.0 37.0 7 35.57577304058885 37.0 35.0 37.0 33.0 37.0 8 35.56361257368447 37.0 35.0 37.0 33.0 37.0 9 37.17882788296815 39.0 37.0 39.0 34.0 39.0 10-11 37.06830454899034 39.0 37.0 39.0 33.0 39.0 12-13 36.931282907900595 39.0 37.0 39.0 33.0 39.0 14-15 38.03976263595937 40.0 38.0 41.0 33.0 41.0 16-17 37.97107589444292 40.0 38.0 41.0 33.0 41.0 18-19 37.91427871277557 40.0 38.0 41.0 33.0 41.0 20-21 37.803158483999624 40.0 38.0 41.0 33.0 41.0 22-23 37.879293483392615 40.0 38.0 41.0 33.0 41.0 24-25 37.844706183875076 40.0 38.0 41.0 33.0 41.0 26-27 37.762995669983496 40.0 38.0 41.0 33.0 41.0 28-29 37.62812329980531 40.0 37.0 41.0 32.5 41.0 30-31 37.3680311689246 40.0 37.0 41.0 32.0 41.0 32-33 37.12368087373708 39.0 36.0 41.0 31.5 41.0 34-35 37.11315596602533 39.5 36.0 41.0 31.0 41.0 36-37 36.99092405160049 39.5 36.0 41.0 31.0 41.0 38-39 36.83956344620753 39.0 36.0 41.0 31.0 41.0 40-41 36.59720167625146 39.0 35.5 41.0 30.0 41.0 42-43 36.33082135422053 39.0 35.0 41.0 30.0 41.0 44-45 36.05433428806525 39.0 35.0 40.5 29.5 41.0 46-47 35.61944303307989 39.0 35.0 40.0 27.5 41.0 48-49 35.26273712798054 39.0 35.0 40.0 26.5 41.0 50-51 34.295048133776376 38.0 34.0 39.5 24.5 40.5 52-53 34.40304450069918 38.0 34.0 39.5 24.5 40.5 54-55 34.714920346581174 38.0 34.0 40.0 24.5 41.0 56-57 34.46190439791188 38.0 34.0 40.0 23.5 41.0 58-59 34.18809267943939 38.0 34.0 40.0 23.0 41.0 60-61 33.86635177000103 38.0 33.5 40.0 22.0 41.0 62-63 33.48796093542745 37.0 33.0 40.0 20.5 41.0 64-65 33.0623030022347 37.0 32.5 39.5 19.0 41.0 66-67 32.61372363433618 36.0 32.0 39.0 17.0 40.5 68-69 32.12361342799717 36.0 31.0 39.0 13.0 40.0 70-71 31.651764155736707 35.0 31.0 38.5 8.0 40.0 72-73 31.152092391670898 35.0 30.5 38.0 7.0 40.0 74-75 30.595575109824146 35.0 30.0 37.0 3.5 39.0 76-77 30.047223258888224 34.0 29.0 36.5 2.0 39.0 78-79 29.478380268074332 34.0 29.0 36.0 2.0 38.5 80-81 28.895934595617824 34.0 28.5 35.5 2.0 37.0 82-83 28.311491405164546 34.0 27.5 35.0 2.0 37.0 84-85 27.69714389773427 33.0 26.0 35.0 2.0 36.0 86-87 27.084469044653574 33.0 25.5 35.0 2.0 36.0 88-89 26.495147279014034 32.5 25.0 35.0 2.0 35.5 90-91 25.89317718895149 32.0 23.5 35.0 2.0 35.0 92-93 25.19815221154581 32.0 19.5 34.0 2.0 35.0 94-95 24.388947216963953 31.0 13.0 34.0 2.0 35.0 96-97 23.4916311077738 31.0 2.0 34.0 2.0 35.0 98-99 22.261854712883483 30.5 2.0 34.0 2.0 35.0 100-101 19.148525636125736 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1832576037452327 1101 2 0.2107430805800945 1101 3 0.10902886423106395 1101 4 -0.043230853529358626 1101 5 0.05999540843748008 1101 6 0.0915640938134743 1101 7 0.1880176966629108 1101 8 0.020905978552704596 1101 9 0.03921112925076642 1101 10-11 0.17633637743839614 1101 12-13 0.2180695871862639 1101 14-15 0.32632304427936987 1101 16-17 0.3509402098250405 1101 18-19 0.3232922564268037 1101 20-21 0.26656128667497114 1101 22-23 0.3236826178678385 1101 24-25 0.3271855361374705 1101 26-27 0.33173408302413776 1101 28-29 0.31565539306809853 1101 30-31 0.335299777895365 1101 32-33 0.3875165499900177 1101 34-35 0.3674116748595395 1101 36-37 0.25892825469473735 1101 38-39 0.39650116789068335 1101 40-41 0.2898981250513444 1101 42-43 0.34952690609974013 1101 44-45 0.2031356981778174 1101 46-47 0.2955835284382857 1101 48-49 0.27702615659283936 1101 50-51 0.3175498629033555 1101 52-53 0.38991005198916895 1101 54-55 0.07141714878904537 1101 56-57 0.369154516961828 1101 58-59 0.4360150371168743 1101 60-61 0.48094303038141817 1101 62-63 0.2504752978705653 1101 64-65 0.2516620568159098 1101 66-67 0.49386150046091615 1101 68-69 0.3519193624243897 1101 70-71 0.4887551050880248 1101 72-73 0.7422792607090614 1101 74-75 0.5362053738966104 1101 76-77 0.563474325540323 1101 78-79 0.7292766029480262 1101 80-81 0.7505849267690365 1101 82-83 0.35297979966266624 1101 84-85 0.6223754728972537 1101 86-87 0.6609519782081392 1101 88-89 1.0406109197796347 1101 90-91 0.83031828268415 1101 92-93 0.8347355048368179 1101 94-95 0.9030567335264088 1101 96-97 0.7367685011590517 1101 98-99 1.0107003744402725 1101 100-101 0.9402801495631898 1102 1 -0.023379867061176896 1102 2 -0.02700515009081883 1102 3 0.04982412894231203 1102 4 0.009324420120513821 1102 5 -0.07293659536688324 1102 6 -0.03976004836509617 1102 7 -0.3088551487343807 1102 8 -0.24297409132115177 1102 9 -0.02415496994066757 1102 10-11 -0.10953324406008136 1102 12-13 -0.18570670748299278 1102 14-15 0.14954819668557917 1102 16-17 0.15595156023804435 1102 18-19 0.15281123328177415 1102 20-21 -0.037479896384162714 1102 22-23 0.05403332246550718 1102 24-25 0.1245213911792078 1102 26-27 0.08285754227029685 1102 28-29 0.26294614622863577 1102 30-31 0.3632738449949784 1102 32-33 0.24476432691692906 1102 34-35 0.544768350346736 1102 36-37 0.5214307414298816 1102 38-39 0.5777862036042833 1102 40-41 0.5453955129574979 1102 42-43 0.3889195159316827 1102 44-45 0.4241635305350826 1102 46-47 0.4657606929879279 1102 48-49 0.3490597200229857 1102 50-51 0.5679606851746897 1102 52-53 0.9044571119188092 1102 54-55 0.9226681468895919 1102 56-57 0.9086002679830898 1102 58-59 0.8003346800080848 1102 60-61 1.0107102719721652 1102 62-63 0.73115779425288 1102 64-65 0.6711804068773688 1102 66-67 0.8998997021630686 1102 68-69 0.8517953663159616 1102 70-71 1.0021875626363865 1102 72-73 1.0396219287237898 1102 74-75 1.1605652950841367 1102 76-77 1.2867068359573572 1102 78-79 1.1288506558766507 1102 80-81 1.36671750177306 1102 82-83 1.2221148518636653 1102 84-85 1.380186546431034 1102 86-87 1.3940196964344764 1102 88-89 1.4288808879221726 1102 90-91 1.6395700274865312 1102 92-93 1.8917369246603926 1102 94-95 1.7527952516230307 1102 96-97 1.9901484170597108 1102 98-99 2.0947792741178795 1102 100-101 2.4124355289504074 1103 1 0.36308925727312413 1103 2 0.24397326189227186 1103 3 0.21833242490432525 1103 4 0.23274580226826913 1103 5 0.23514219979644935 1103 6 0.34269525511930254 1103 7 0.1934561718722989 1103 8 0.319651242510119 1103 9 0.29046393675604065 1103 10-11 0.4125584618608542 1103 12-13 0.3154607212552136 1103 14-15 0.16654966224799495 1103 16-17 0.36221770260785746 1103 18-19 0.2519704962953142 1103 20-21 0.11229462040390104 1103 22-23 0.26787519863646736 1103 24-25 0.5665422354310294 1103 26-27 0.3545546133422235 1103 28-29 0.39825019551170016 1103 30-31 0.3972814834937566 1103 32-33 0.2903301375737968 1103 34-35 0.2786856072657997 1103 36-37 0.3328298043486342 1103 38-39 0.6317248633835462 1103 40-41 0.7644013783454326 1103 42-43 0.5909960954910005 1103 44-45 0.7218484440292343 1103 46-47 0.6041198279608935 1103 48-49 0.384674142161451 1103 50-51 0.7062821611561034 1103 52-53 0.8033380122197684 1103 54-55 1.1335961585889862 1103 56-57 1.1266379834631408 1103 58-59 1.1237344082356913 1103 60-61 0.9918541010756527 1103 62-63 1.2535743089759706 1103 64-65 0.9944530360084798 1103 66-67 1.209792405419627 1103 68-69 1.163597694269285 1103 70-71 1.3673981862379065 1103 72-73 1.272020272983461 1103 74-75 1.1682775427296654 1103 76-77 0.9897374433027437 1103 78-79 1.2015842906911338 1103 80-81 0.9768527917002174 1103 82-83 1.1795256706642903 1103 84-85 1.5923525974777952 1103 86-87 1.5200204056788174 1103 88-89 1.5590225171129113 1103 90-91 1.2355722117080425 1103 92-93 1.4114482684901226 1103 94-95 1.4213600360723753 1103 96-97 1.8113984445193942 1103 98-99 1.582508719369553 1103 100-101 1.6756373591773936 1104 1 0.06182418248922161 1104 2 0.2381649436021931 1104 3 0.05617513189773149 1104 4 0.1343322802726874 1104 5 0.006293738532384907 1104 6 0.11102911091303014 1104 7 0.08949736337024916 1104 8 0.08438552636104646 1104 9 -0.1477794433104691 1104 10-11 0.17418680876014037 1104 12-13 0.03261687926164569 1104 14-15 0.12002546512549372 1104 16-17 0.08134299581623594 1104 18-19 0.17592008066906573 1104 20-21 0.07932196489986865 1104 22-23 0.24905941817072375 1104 24-25 0.09902305753146123 1104 26-27 -0.06516484344309248 1104 28-29 0.2732272252703254 1104 30-31 0.2732908229236699 1104 32-33 0.1830149714494027 1104 34-35 0.27900088508860676 1104 36-37 0.24720575243409115 1104 38-39 0.10149242255668156 1104 40-41 0.211276164406911 1104 42-43 -0.18927327788080817 1104 44-45 -0.06659293051001214 1104 46-47 0.06423267940559185 1104 48-49 -0.10016369694236715 1104 50-51 -0.11725594061918088 1104 52-53 0.0024260485974210155 1104 54-55 0.3674584380296295 1104 56-57 0.2501710407932549 1104 58-59 0.10820112030236828 1104 60-61 0.16725306264059014 1104 62-63 0.15406542174454785 1104 64-65 0.20227516887195662 1104 66-67 0.35415361651885036 1104 68-69 0.3093191039755183 1104 70-71 0.2565735275486709 1104 72-73 0.34799141875711825 1104 74-75 0.30786454679765285 1104 76-77 0.26111418048354906 1104 78-79 0.4488846117340337 1104 80-81 0.6689560731118007 1104 82-83 0.7872912336784204 1104 84-85 0.39015321938582304 1104 86-87 -0.14185403808102492 1104 88-89 -0.009841365740658858 1104 90-91 0.17657562391488213 1104 92-93 0.6304654664449636 1104 94-95 0.7816577304006138 1104 96-97 0.7585254528391268 1104 98-99 1.0408271896055261 1104 100-101 1.267934491410319 1105 1 0.15925258254920038 1105 2 -0.10840125822716473 1105 3 0.0400182867935186 1105 4 0.12526045541139297 1105 5 0.06960907816350925 1105 6 0.2367226181854818 1105 7 0.10606656414997673 1105 8 0.19354731973336925 1105 9 0.19638759822499452 1105 10-11 0.1898188375500638 1105 12-13 0.26694736359213067 1105 14-15 0.5329809307125402 1105 16-17 0.42704699941497637 1105 18-19 0.44278917648552607 1105 20-21 0.527908709539247 1105 22-23 0.5677353815835247 1105 24-25 0.6193409462704054 1105 26-27 0.5024427439013408 1105 28-29 0.32209137281868294 1105 30-31 0.31090890659437775 1105 32-33 0.050911207766695554 1105 34-35 0.37867849792411334 1105 36-37 0.671426025338576 1105 38-39 0.48935042225678416 1105 40-41 0.32701690514765147 1105 42-43 0.4588373833877455 1105 44-45 0.5887714419596222 1105 46-47 0.6125345237600612 1105 48-49 0.4773029397347912 1105 50-51 0.5559709299761053 1105 52-53 0.5332027767425771 1105 54-55 0.8466157329763959 1105 56-57 0.7046057386490077 1105 58-59 0.18366220892134777 1105 60-61 0.27798930495577423 1105 62-63 0.1339537108433575 1105 64-65 0.441891303224935 1105 66-67 0.5326848805764328 1105 68-69 0.555739106974471 1105 70-71 0.6274124839139432 1105 72-73 0.73146345354494 1105 74-75 1.127327734681895 1105 76-77 1.0071968015661668 1105 78-79 1.0598832622063732 1105 80-81 0.9595356202702945 1105 82-83 1.3671945175290716 1105 84-85 1.390340653783781 1105 86-87 1.208929437702455 1105 88-89 1.2112749935335962 1105 90-91 1.604019768464287 1105 92-93 1.6498086963145049 1105 94-95 2.093848463643983 1105 96-97 1.9815723864123775 1105 98-99 2.250172668687849 1105 100-101 2.8396281486302897 1106 1 -0.08984547182523528 1106 2 -0.7644700637934569 1106 3 -0.08673728594195396 1106 4 0.08948327893069319 1106 5 -0.07256546978992873 1106 6 -0.32616661430174076 1106 7 -0.16640691435365085 1106 8 -0.028954076134859008 1106 9 0.22871805392227884 1106 10-11 -0.4629535715037534 1106 12-13 -0.3357992755866732 1106 14-15 -0.10274041103895115 1106 16-17 -0.15758556511708832 1106 18-19 0.3198561352107774 1106 20-21 0.0223950300305944 1106 22-23 0.07114989002315752 1106 24-25 -0.043717937894008685 1106 26-27 0.14455823589119632 1106 28-29 0.2513047473838199 1106 30-31 0.37419005593081067 1106 32-33 0.0606071232415033 1106 34-35 0.3162509756975993 1106 36-37 0.07881016827548137 1106 38-39 0.07731696436906077 1106 40-41 0.4997555986149891 1106 42-43 0.31387109897422505 1106 44-45 0.5567577409760744 1106 46-47 0.6890012591463943 1106 48-49 0.8954674803867633 1106 50-51 0.5868518302037415 1106 52-53 0.39544131988564146 1106 54-55 0.33679096812650755 1106 56-57 0.6622411378673192 1106 58-59 0.6742991543321679 1106 60-61 0.5430109946005857 1106 62-63 0.9329647918896171 1106 64-65 0.846671615040421 1106 66-67 1.0534027863445843 1106 68-69 1.053446661842834 1106 70-71 1.5151470971510719 1106 72-73 1.189614250263574 1106 74-75 0.6767092576815052 1106 76-77 1.1573546420506418 1106 78-79 1.3079827339239323 1106 80-81 1.2689835006980736 1106 82-83 1.226732592519408 1106 84-85 0.8175729692070526 1106 86-87 0.9623707897618949 1106 88-89 1.5789627914977764 1106 90-91 1.5610001833592726 1106 92-93 2.041328259944624 1106 94-95 1.6551700287343891 1106 96-97 1.8989155122982133 1106 98-99 0.6591674288132587 1106 100-101 0.7619910314203082 1107 1 0.24904163510421284 1107 2 0.15923831260389676 1107 3 0.0889817346931352 1107 4 0.2561024266788934 1107 5 -0.0049044697542939275 1107 6 -0.14511953493326502 1107 7 0.11197672963461258 1107 8 -0.047587432039684074 1107 9 0.15042469587911 1107 10-11 0.010195269628624715 1107 12-13 0.19888953489600425 1107 14-15 0.10353921403677901 1107 16-17 0.21037124018602782 1107 18-19 0.17542238252298858 1107 20-21 0.3083460617851088 1107 22-23 0.12797360671536495 1107 24-25 0.05532930779067868 1107 26-27 0.16835927417957208 1107 28-29 0.0740235423040474 1107 30-31 0.037381901684398144 1107 32-33 0.4639863123311656 1107 34-35 0.5882165957600876 1107 36-37 0.5204212972061839 1107 38-39 0.4490931027441505 1107 40-41 0.46738333540769617 1107 42-43 0.5462623697489022 1107 44-45 0.878846910226585 1107 46-47 1.077732741617858 1107 48-49 0.7272802083290841 1107 50-51 0.5426503262299676 1107 52-53 0.8199367283701449 1107 54-55 0.8307946746021457 1107 56-57 0.8952085755284429 1107 58-59 0.7089849948824352 1107 60-61 0.8342722654940573 1107 62-63 0.9618087754005558 1107 64-65 0.6698418396861072 1107 66-67 0.922960385213635 1107 68-69 0.9952508363798174 1107 70-71 1.0097774593640239 1107 72-73 1.200071382259253 1107 74-75 1.1908847450474127 1107 76-77 1.2607201694512362 1107 78-79 1.006555384410408 1107 80-81 1.2422006502757483 1107 82-83 0.9688266324590593 1107 84-85 1.314917849637574 1107 86-87 0.9156715726360751 1107 88-89 1.0066241479253044 1107 90-91 1.3836387901045875 1107 92-93 1.3706670341941205 1107 94-95 1.0693894710998855 1107 96-97 1.1043219772470714 1107 98-99 1.3113883442652252 1107 100-101 1.3769303854006125 1108 1 0.1650853383819566 1108 2 0.15476575493985223 1108 3 -0.0917095949343647 1108 4 0.10735793750887268 1108 5 0.2511175096719427 1108 6 0.16597879744166022 1108 7 0.3448053028887159 1108 8 0.10946323564928662 1108 9 0.14735779919519132 1108 10-11 0.15545755318877497 1108 12-13 0.2478320944768626 1108 14-15 -0.055492157760546945 1108 16-17 -0.1253900530220804 1108 18-19 -0.1676866839903326 1108 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0.36101012116927933 2310 32-33 0.27853154065018515 2310 34-35 0.2590203782660012 2310 36-37 0.6151394756309685 2310 38-39 0.38999144789781326 2310 40-41 0.4578648583640259 2310 42-43 0.597276429721525 2310 44-45 0.20241291744320478 2310 46-47 0.05838472618921031 2310 48-49 -0.02365297569480873 2310 50-51 -0.08606685679851722 2310 52-53 0.19784515191125962 2310 54-55 0.1754506407586689 2310 56-57 0.29159404274257383 2310 58-59 0.1973824068520713 2310 60-61 0.1857715811591092 2310 62-63 -0.03501839891822556 2310 64-65 0.08044281019749633 2310 66-67 0.6889489381562797 2310 68-69 0.7050306049501742 2310 70-71 0.7536495509404801 2310 72-73 0.4829816721684246 2310 74-75 0.876090667655351 2310 76-77 0.8205683715166856 2310 78-79 1.0102881205059653 2310 80-81 1.1460515896810008 2310 82-83 1.7638994208129226 2310 84-85 1.8618881121733466 2310 86-87 1.8094130184491917 2310 88-89 1.8946420650585623 2310 90-91 2.1829109065659544 2310 92-93 2.5368982149829726 2310 94-95 2.818207438002954 2310 96-97 3.383281788121778 2310 98-99 3.5247453182604964 2310 100-101 4.017934491410319 2311 1 0.4380987363953537 2311 2 0.22172694690104322 2311 3 0.1906593124345406 2311 4 0.26201259216352923 2311 5 0.06213044568487902 2311 6 0.14163714810001693 2311 7 0.5024340855174998 2311 8 0.21064133682738628 2311 9 0.37053289737028905 2311 10-11 0.30573764096886435 2311 12-13 0.49985334649811364 2311 14-15 0.5116134093450242 2311 16-17 0.4553589652269423 2311 18-19 0.447062680759025 2311 20-21 0.5556864873170255 2311 22-23 0.42831230466045156 2311 24-25 0.5328024847319384 2311 26-27 0.6071435986021925 2311 28-29 0.5881170138443252 2311 30-31 0.5336653168507866 2311 32-33 0.8329624898179802 2311 34-35 0.8322041389497628 2311 36-37 0.8786909826035298 2311 38-39 0.645333328239694 2311 40-41 0.7869528025835493 2311 42-43 0.9775339645843317 2311 44-45 0.8831090487972375 2311 46-47 0.9079405066660442 2311 48-49 0.4821106320424846 2311 50-51 0.6644111009162756 2311 52-53 0.9183523493921442 2311 54-55 1.0148849637456294 2311 56-57 -0.20992417588091428 2311 58-59 0.5271451149042505 2311 60-61 0.6919850314515088 2311 62-63 1.1676075569972113 2311 64-65 1.1277887391223658 2311 66-67 1.1667660771576251 2311 68-69 1.4472989360342936 2311 70-71 1.559036415537875 2311 72-73 1.5220617441432296 2311 74-75 1.625725170579333 2311 76-77 1.7876455195148857 2311 78-79 1.909242236565344 2311 80-81 1.9242792100138857 2311 82-83 1.8575791329136884 2311 84-85 2.1018791153222445 2311 86-87 2.21266875394177 2311 88-89 2.6968519166105196 2311 90-91 2.8283787428232614 2311 92-93 3.233676217682028 2311 94-95 3.3673527371482557 2311 96-97 3.411059565899553 2311 98-99 3.810001728516905 2311 100-101 4.694329003331145 2312 1 0.35960140922311723 2312 2 0.2766897991253785 2312 3 0.26197624638398764 2312 4 0.26613508921888496 2312 5 0.3463222550533658 2312 6 0.33361639970824086 2312 7 0.14709522531997976 2312 8 0.28856106139021875 2312 9 0.3564325983759957 2312 10-11 0.4757283540249446 2312 12-13 0.5723368112077267 2312 14-15 0.6241892904721382 2312 16-17 0.5047100117069618 2312 18-19 0.6479098312528251 2312 20-21 0.7902271233594291 2312 22-23 0.2168474591408298 2312 24-25 0.2982391976068186 2312 26-27 0.18903858818269725 2312 28-29 0.1461943899428988 2312 30-31 0.6577989129366841 2312 32-33 0.4199879496349581 2312 34-35 0.26893400213721463 2312 36-37 0.36896234257454097 2312 38-39 0.6783699323893728 2312 40-41 0.8901794493172659 2312 42-43 0.9432458881142693 2312 44-45 1.16375030094909 2312 46-47 1.475242536203055 2312 48-49 1.6578660456512537 2312 50-51 1.9374076126495368 2312 52-53 1.309281723316218 2312 54-55 1.4260757311643104 2312 56-57 1.5717533633054614 2312 58-59 1.845353187750142 2312 60-61 1.9361693659626908 2312 62-63 1.8042558762868666 2312 64-65 1.8981747135719473 2312 66-67 2.0135258397742817 2312 68-69 2.557819231404416 2312 70-71 2.881395299292514 2312 72-73 2.906054223983773 2312 74-75 2.7534489632766146 2312 76-77 2.769620920439049 2312 78-79 2.9907295066568516 2312 80-81 2.8531321591848524 2312 82-83 3.1487588013018346 2312 84-85 3.3768179573988917 2312 86-87 3.4004179158517083 2312 88-89 3.1692685606261044 2312 90-91 3.294957070267312 2312 92-93 3.139464701871571 2312 94-95 3.3092753610496786 2312 96-97 3.2223080935791764 2312 98-99 3.4249219967057236 2312 100-101 3.777718964371285 2313 1 0.15506777364740998 2313 2 0.1250530233403282 2313 3 0.13322099603396254 2313 4 0.20226416449057183 2313 5 0.24131525458666658 2313 6 0.23903676106566252 2313 7 0.39516509664473887 2313 8 -0.006871967478375041 2313 9 -0.046194243413452796 2313 10-11 0.17366821668246502 2313 12-13 0.2379278506103546 2313 14-15 0.48713348675188684 2313 16-17 0.13277188249834637 2313 18-19 0.25603703973339265 2313 20-21 0.6014412042981618 2313 22-23 0.5597769877760754 2313 24-25 0.5170671194682726 2313 26-27 0.29670576599454535 2313 28-29 0.20717845554727887 2313 30-31 0.6852074068362768 2313 32-33 0.506160361129055 2313 34-35 0.3280979464001703 2313 36-37 0.6606817905377369 2313 38-39 0.6111654520906811 2313 40-41 0.30296030137406404 2313 42-43 0.47121441934968544 2313 44-45 0.4763178054493835 2313 46-47 0.6308662444502744 2313 48-49 0.40445581830276467 2313 50-51 0.4929246471185067 2313 52-53 0.5643187257830462 2313 54-55 0.8123148137698166 2313 56-57 0.708955843470811 2313 58-59 0.6559005575747889 2313 60-61 0.942849811325722 2313 62-63 0.7844012193097427 2313 64-65 1.1622589858567665 2313 66-67 1.4599022648692852 2313 68-69 1.1593393327445085 2313 70-71 1.0650475427754174 2313 72-73 1.5114545840077724 2313 74-75 1.4758037869315253 2313 76-77 1.5085635166121278 2313 78-79 1.6334923575135782 2313 80-81 1.6754403130617774 2313 82-83 1.8078694086756606 2313 84-85 2.1307615536386457 2313 86-87 2.4586351303326737 2313 88-89 2.542540112062863 2313 90-91 2.4124982396786088 2313 92-93 2.556759188170659 2313 94-95 2.044517710539644 2313 96-97 2.8679778048932647 2313 98-99 3.0955000352416313 2313 100-101 3.2252554668047786 2314 1 -0.13131866055368846 2314 2 -0.3150711705932778 2314 3 -0.07698917831521257 2314 4 -0.08081441205587936 2314 5 -0.08242489349071747 2314 6 -0.07740699341248103 2314 7 0.16548991154800774 2314 8 0.04235182043660046 2314 9 0.13842481557866648 2314 10-11 -0.07252834410090969 2314 12-13 0.13816071423585896 2314 14-15 0.25605514903992344 2314 16-17 0.21552206162422038 2314 18-19 -0.0420642260894013 2314 20-21 0.1569523101018362 2314 22-23 0.2961514802527887 2314 24-25 0.2729842439010426 2314 26-27 0.07809458854052309 2314 28-29 0.5453143523707737 2314 30-31 0.48090241519548727 2314 32-33 0.11951717985368049 2314 34-35 0.35360546968652784 2314 36-37 0.28675654573238774 2314 38-39 0.31017428896672783 2314 40-41 0.6861941203375252 2314 42-43 0.8398554511146799 2314 44-45 1.0761267378592265 2314 46-47 0.6705784488925985 2314 48-49 0.5266375713478979 2314 50-51 0.7447218766384438 2314 52-53 0.5223973023295017 2314 54-55 0.44248558042852437 2314 56-57 0.5636071451187661 2314 58-59 0.5986519308730962 2314 60-61 0.3341263816624789 2314 62-63 0.2182201827680572 2314 64-65 0.5923700438935455 2314 66-67 0.9306211566772689 2314 68-69 1.2247859532365126 2314 70-71 0.9376472561740314 2314 72-73 0.862941328693708 2314 74-75 0.8411787570772198 2314 76-77 1.0269955682003804 2314 78-79 1.0673889421804041 2314 80-81 0.8605012158561429 2314 82-83 1.048913115386906 2314 84-85 0.8421420169775473 2314 86-87 1.35943522586647 2314 88-89 1.492271423264203 2314 90-91 1.4539061689836004 2314 92-93 1.8481074088537248 2314 94-95 1.5288262846977538 2314 96-97 2.2554688485999783 2314 98-99 2.679808609399739 2314 100-101 3.398726926083498 2315 1 0.07491089427492881 2315 2 0.23602397945649756 2315 3 0.18406899264197563 2315 4 -0.0039050108327245425 2315 5 0.24057325755465797 2315 6 0.13209378883755107 2315 7 0.1675522070962927 2315 8 0.1966684183600833 2315 9 0.21657870549474723 2315 10-11 0.18536526989424829 2315 12-13 0.2505736000273586 2315 14-15 0.10762320479301479 2315 16-17 0.22594020982503338 2315 18-19 0.2554752419805837 2315 20-21 0.3618662625979212 2315 22-23 0.39771953600588006 2315 24-25 0.4257007849336176 2315 26-27 0.219575174516919 2315 28-29 0.4974370945127191 2315 30-31 0.05640732203661969 2315 32-33 0.4258391824137746 2315 34-35 0.16054971659308848 2315 36-37 0.4248592338279593 2315 38-39 0.3700164166868305 2315 40-41 0.29892701750720363 2315 42-43 0.4085156701446877 2315 44-45 0.31549524873960877 2315 46-47 0.1394840099792134 2315 48-49 0.31434557874371905 2315 50-51 0.30952418072036636 2315 52-53 0.5157126202074735 2315 54-55 0.4090977266381657 2315 56-57 0.7909041156777192 2315 58-59 1.1298352618359715 2315 60-61 0.8031741700282851 2315 62-63 0.9553308355912691 2315 64-65 0.979992714921849 2315 66-67 0.9719915165122828 2315 68-69 1.1131553174805688 2315 70-71 1.0086186684909109 2315 72-73 0.9375039798101881 2315 74-75 1.154985470204796 2315 76-77 1.5853443094889563 2315 78-79 1.8536746767843049 2315 80-81 1.9827277839118977 2315 82-83 2.1499300404331336 2315 84-85 2.317198907889228 2315 86-87 2.1751975640829393 2315 88-89 2.2793749647234556 2315 90-91 2.486618443197795 2315 92-93 3.0363807696843317 2315 94-95 3.1869652407495224 2315 96-97 3.4213591913677206 2315 98-99 3.387104868822295 2315 100-101 3.850191867059177 2316 1 -0.041158641446926936 2316 2 -0.11052649148447458 2316 3 -0.23119948914123967 2316 4 -0.1211859702803153 2316 5 0.24401067237642593 2316 6 0.2697645238689006 2316 7 0.13047506969056144 2316 8 0.29447574141650534 2316 9 0.16404834418762704 2316 10-11 0.01571673420267672 2316 12-13 -0.21705867230070197 2316 14-15 -0.35976678278989027 2316 16-17 -0.2707321606283344 2316 18-19 -0.14881471228817134 2316 20-21 -0.2492326501492812 2316 22-23 -0.2782284030595079 2316 24-25 -0.03630215276891846 2316 26-27 -0.34372550085181786 2316 28-29 -0.1763794959352012 2316 30-31 -0.013983967826796118 2316 32-33 -0.30381268469304246 2316 34-35 0.30896048009289245 2316 36-37 0.24154432423046046 2316 38-39 0.6313810855138655 2316 40-41 0.4601031585169224 2316 42-43 0.44291253255723717 2316 44-45 0.11567090517984013 2316 46-47 0.39151314471981635 2316 48-49 0.38450972665855687 2316 50-51 0.5163747818893896 2316 52-53 0.4923510707965235 2316 54-55 0.31420160622265314 2316 56-57 0.5698722855193239 2316 58-59 0.4049571613483778 2316 60-61 0.2897500808674991 2316 62-63 0.6569079578542159 2316 64-65 0.8644153200885683 2316 66-67 0.6437297581307391 2316 68-69 0.5520617047121661 2316 70-71 0.5302766532875438 2316 72-73 0.5677888158136994 2316 74-75 0.7132700942091574 2316 76-77 0.6619327888493238 2316 78-79 0.933492357513579 2316 80-81 0.9282705017410251 2316 82-83 1.2553087617753924 2316 84-85 1.583322200538916 2316 86-87 1.7502793351844268 2316 88-89 1.7223244786396847 2316 90-91 1.6375656251233544 2316 92-93 1.6888346598687711 2316 94-95 2.0833317267121494 2316 96-97 2.359622009745017 2316 98-99 2.405473081063729 2316 100-101 2.23226355305815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1706.0 3 307.0 4 311.0 5 336.0 6 376.0 7 451.0 8 575.0 9 734.0 10 924.0 11 1233.0 12 1665.0 13 2054.0 14 2421.0 15 3204.0 16 4130.0 17 5398.0 18 4649.0 19 3237.0 20 3158.0 21 3396.0 22 3724.0 23 4261.0 24 4715.0 25 5488.0 26 6529.0 27 7294.0 28 8659.0 29 10509.0 30 12511.0 31 15732.0 32 19481.0 33 25283.0 34 33586.0 35 45842.0 36 63126.0 37 78951.0 38 53845.0 39 5001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34052288612947 20.465318616125668 15.509643798193634 21.684514699551226 2 14.572653713296846 21.060529926971114 41.43200607484954 22.934810284882502 3 18.309808375245616 26.21996286481712 30.810484379788367 24.6597443801489 4 11.889922396776898 17.51886001676446 39.122029040369895 31.469188546088745 5 12.690937523236975 38.49823229990289 35.018262854413166 13.792567322446974 6 29.725023596115598 38.234267191375196 18.199081384635832 13.841627827873376 7 27.118605887494258 34.134896964345245 21.455249096692228 17.29124805146827 8 25.803188877225075 35.694880586054175 20.25126262057407 18.250667916146675 9 26.22072930606433 17.00234751303298 21.010016355992917 35.766906824909775 10-11 23.97845210696893 27.83449758194519 28.007078992043454 20.179971319042423 12-13 25.137367898152885 25.420664547480065 28.489505070052555 20.95246248431449 14-15 22.344541421267998 26.552502080890612 27.099897391537446 24.003059106303944 16-17 20.864620371423648 30.14412967503528 27.640978914967206 21.35027103857386 18-19 20.949505505767036 28.162634659867695 30.74152738018284 20.14633245418242 20-21 21.73419961100844 27.25733920311914 30.812095903200337 20.196365282672083 22-23 21.52285773169405 27.00470409899668 30.623605635008317 20.848832534300957 24-25 20.972255852061245 27.42982312894525 30.822864286237166 20.77505673275634 26-27 20.90399043215229 27.600245052219996 30.725449168556736 20.770315347070973 28-29 20.815772104621747 27.44180860194223 31.067775267332397 20.674644026103625 30-31 20.972136955285077 27.54031326916253 30.739578815750196 20.747970959802192 32-33 20.716175620833095 27.715282400633477 30.616840073034552 20.95170190549888 34-35 21.247710679441056 27.58988891867477 30.453538900990967 20.7088615008932 36-37 21.253522300583164 27.390490520478334 30.432902285581715 20.923084893356787 38-39 20.961137825024494 27.802727446762987 30.493350300109235 20.742784428103285 40-41 21.084683313101465 27.72651620763976 30.494303171711117 20.69449730754765 42-43 21.013077636925516 27.72950059013794 30.111788343199 21.145633429737547 44-45 21.17612743088559 28.002653308527076 29.928779613220595 20.892439647366736 46-47 21.23731241749974 28.247075042010373 29.40219129698291 21.113421243506977 48-49 21.186689809209184 28.410136613837544 29.170979612110866 21.23219396484241 50-51 21.33126063104773 28.38746015117209 29.12777533709574 21.153503880684443 52-53 21.407746537580238 28.55575357620027 28.940016267233243 21.09648361898625 54-55 21.323763793664686 28.439982563974333 28.98874430203522 21.247509340325767 56-57 21.238073037548045 28.873919070640046 28.791600085134274 21.096407806677636 58-59 21.211834586042848 28.36888776285787 29.00261802212651 21.416659628972777 60-61 21.30150329373346 28.486121276955128 29.202747593040932 21.009627836270482 62-63 21.207412036958836 28.513257095882388 29.299617686638175 20.979713180520598 64-65 21.21087574374935 28.415842030114813 29.1805357247418 21.192746501394037 66-67 20.99414104947621 28.604245515138345 28.96560189089943 21.43601154448602 68-69 20.949855297681385 28.467281510759772 29.167370471718296 21.415492719840547 70-71 21.145552044647342 28.503444718960928 29.049487289786562 21.301515946605168 72-73 21.121093503662127 28.501657642723938 28.864830046576156 21.512418807037776 74-75 21.084412652044016 28.826565373436203 28.700999906529738 21.38802206799004 76-77 21.05346143926587 28.576400112173406 28.93409361974342 21.436044828817302 78-79 21.169877534287924 28.629316539065368 28.726284515982258 21.47452141066445 80-81 20.947957818489773 28.859581157964442 28.549436243485154 21.64302478006063 82-83 21.18654851630761 28.822967024428475 28.573986425996683 21.41649803326723 84-85 21.228369650401305 28.455113365101386 28.591696467981226 21.724820516516083 86-87 21.01090991801516 28.49741749437839 28.800089180050243 21.69158340755621 88-89 21.306319443897046 28.23452175979154 28.74148261840441 21.717676177907002 90-91 21.122524356681815 28.381333525514275 28.86024692247751 21.6358951953264 92-93 21.25708751639912 28.198152037432223 28.750063344237926 21.794697101930733 94-95 21.029031382630716 28.273420424102998 28.587708422715668 22.109839770550618 96-97 21.150097356125215 28.22437968550987 28.640381451321375 21.985141507043537 98-99 21.135311672954558 28.134579649755054 28.502393152767613 22.227715524522775 100-101 21.458629158394828 27.474711531856773 28.981378363678743 22.08528094606966 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 605.0 1 350.0 2 104.5 3 121.0 4 106.0 5 98.0 6 172.5 7 362.0 8 653.5 9 857.0 10 793.5 11 679.0 12 697.5 13 732.5 14 754.0 15 775.0 16 805.5 17 873.5 18 915.0 19 915.5 20 1003.0 21 1154.0 22 1327.0 23 1637.5 24 1999.5 25 2402.5 26 3120.5 27 3984.0 28 4662.0 29 5358.5 30 6242.5 31 7459.0 32 8765.5 33 10076.0 34 11440.5 35 12633.5 36 14215.0 37 15545.5 38 15922.5 39 15952.0 40 15628.5 41 15674.0 42 15929.5 43 16195.5 44 16664.0 45 16776.5 46 16497.5 47 16316.0 48 16612.0 49 15983.5 50 14772.5 51 13683.5 52 12535.5 53 11961.5 54 11513.0 55 10355.5 56 8877.0 57 7852.5 58 7148.0 59 6277.5 60 5188.0 61 4137.0 62 3161.0 63 2386.0 64 1809.0 65 1354.5 66 978.0 67 705.0 68 517.0 69 343.0 70 244.0 71 194.5 72 152.0 73 123.5 74 88.0 75 59.5 76 46.0 77 26.0 78 13.0 79 11.5 80 10.5 81 8.5 82 6.5 83 3.0 84 1.5 85 1.0 86 2.0 87 1.5 88 0.5 89 1.5 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2583171838256123 2 0.22594322867253291 3 0.2288658774016304 4 0.22616804780554045 5 0.22594322867253291 6 0.19626710311554355 7 0.19739119878058103 8 0.198740113578626 9 0.20863215543095578 10-11 0.21436504332264694 12-13 0.20705842149990333 14-15 0.19817806574610725 16-17 0.19368168308595735 18-19 0.18277795513509382 20-21 0.18738674736174746 22-23 0.1860378325637025 24-25 0.18761156649475497 26-27 0.1832275934011088 28-29 0.19761601791358852 30-31 0.2100934797955045 32-33 0.20289926753926465 34-35 0.20076348577569345 36-37 0.18997216739133366 38-39 0.1791808490069739 40-41 0.17603338114486894 42-43 0.1883984334602812 44-45 0.18615024213020626 46-47 0.19424373091847608 48-49 0.19930216141114473 50-51 0.21065552762802325 52-53 0.21695046335223314 54-55 0.20087589534219719 56-57 0.17929325857347764 58-59 0.1718742271842303 60-61 0.17468446634682397 62-63 0.17861880117445514 64-65 0.17266109414975653 66-67 0.1754713333123502 68-69 0.17693265767689895 70-71 0.1773822959429139 72-73 0.17940566813998138 74-75 0.1822159073025751 76-77 0.1903093960908449 78-79 0.18907289085930368 80-81 0.1834524125341163 82-83 0.17794434377543267 84-85 0.16861434975562162 86-87 0.17007567412017033 88-89 0.17614579071137268 90-91 0.1744596472138165 92-93 0.17974289683949263 94-95 0.1759209715783652 96-97 0.18289036470159759 98-99 0.18592542299719875 100-101 0.17434723764731275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 444802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.91230519431657 #Duplication Level Percentage of deduplicated Percentage of total 1 84.1692814217189 55.477913650546576 2 9.16709372918624 12.084485592460583 3 2.670155266291787 5.279882664841079 4 1.1938355562889322 3.147538141517713 5 0.6415574898788108 2.1143266536295924 6 0.42524971831557573 1.6817513530448032 7 0.3094362888599008 1.4276961376671342 8 0.2159736954597146 1.1388259303268076 9 0.18309529769271735 1.0861409827049968 >10 0.956258327579188 11.481433972004659 >50 0.05369810944087376 2.4358504151832827 >100 0.012996994593412055 1.2554605204466995 >500 3.4202617351084355E-4 0.13751677455063507 >1k 0.0010260785205325304 1.2511772110754502 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2368 0.5323717069617493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1943 0.4368235754335637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.2785509057962869 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 610 0.13713967113457223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1240956650374773E-4 0.0 22-23 0.0 0.0 0.0 2.2481913300749547E-4 0.0 24-25 0.0 0.0 0.0 2.2481913300749547E-4 0.0 26-27 0.0 0.0 0.0 6.744573990224864E-4 0.0 28-29 0.0 0.0 0.0 0.0011240956650374774 0.0 30-31 0.0 0.0 0.0 0.0024730104630824503 0.0 32-33 0.0 0.0 0.0 0.004608792226653657 0.0 34-35 0.0 0.0 0.0 0.006070116591202377 0.0 36-37 0.0 0.0 0.0 0.007194212256239855 0.0 38-39 0.0 0.0 0.0 0.009892041852329801 0.0 40-41 0.0 0.0 0.0 0.01337673841394598 0.0 42-43 0.0 0.0 0.0 0.02180745590172706 0.0 44-45 0.0 0.0 0.0 0.03585865171469553 0.0 46-47 0.0 0.0 0.0 0.04889816142913026 0.0 48-49 0.0 0.0 0.0 0.08419476531130705 0.0 50-51 0.0 0.0 0.0 0.1260111240507012 0.0 52-53 0.0 0.0 0.0 0.16029604183434426 0.0 54-55 0.0 0.0 0.0 0.2124540806920832 0.0 56-57 0.0 0.0 0.0 0.2747289805351595 0.0 58-59 0.0 0.0 0.0 0.35813687888094026 0.0 60-61 0.0 0.0 0.0 0.4530105530101034 0.0 62-63 0.0 0.0 0.0 0.5877896232480969 0.0 64-65 0.0 0.0 0.0 0.7141379759983093 0.0 66-67 0.0 0.0 0.0 0.8887100327786296 0.0 68-69 0.0 0.0 0.0 1.116226995382215 0.0 70-71 0.0 0.0 0.0 1.2768602659160706 0.0 72-73 0.0 0.0 0.0 1.4997684362930022 0.0 74-75 0.0 0.0 0.0 1.7933822240007915 0.0 76-77 0.0 0.0 0.0 2.036861345047909 1.1240956650374773E-4 78-79 0.0 0.0 0.0 2.266401679848562 2.2481913300749547E-4 80-81 0.0 0.0 0.0 2.594750023606009 2.2481913300749547E-4 82-83 0.0 0.0 0.0 2.996164585590892 2.2481913300749547E-4 84-85 0.0 0.0 0.0 3.3806053030337093 2.2481913300749547E-4 86-87 0.0 0.0 0.0 3.873184023453132 2.2481913300749547E-4 88-89 0.0 0.0 0.0 4.475586890346716 2.2481913300749547E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1335 0.0 43.068577 1 GTATCAA 2190 0.0 36.452023 1 GTACTAG 55 0.0025731889 34.55841 1 TGGACCG 55 0.002576036 34.55062 5 TATCAAC 2790 0.0 28.09243 2 AGGGCCG 85 6.395337E-4 27.945354 5 ATCAACG 2815 0.0 27.842941 3 TCAACGC 2880 0.0 27.21761 4 CGCTCCG 35 0.008389052 27.143854 48-49 CAACGCA 2905 0.0 26.98338 5 AACGCAG 2915 0.0 26.88475 6 GTCTAGG 95 0.0012226916 25.009377 1 GTACATG 2065 0.0 24.161598 1 GGACCGA 100 0.0016527819 23.748196 6 CGCAGAG 3325 0.0 23.283947 8 TACATGG 2095 0.0 23.127342 2 ACGCAGA 3350 0.0 23.110186 7 TAAGGTG 250 3.8016879E-10 22.803408 5 ACATGGG 2115 0.0 22.684052 3 GAGTACT 1865 0.0 22.156494 12-13 >>END_MODULE