FastQCFastQC Report
Thu 26 May 2016
SRR937275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444802
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.6591696979779768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.5777851718292634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17530.39410794016213957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.1335425650064523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.046.2614171
GTATCAA27550.033.1090131
ATAGCCC1004.9482915E-528.4983833
GTATAGG1703.621608E-927.9458711
CTCGCCA856.403085E-427.9395894
TAACGTA350.00838829127.14436786-87
CCTAGAC1405.7955185E-727.1413153
ATCAACG34050.026.5036333
ACGCGAA458.943046E-426.39035678-79
TAACGCG458.943046E-426.39035676-77
TCAACGC34500.026.1579344
TATCAAC35050.025.7474672
CAACGCA35050.025.7474675
GTACATG19750.025.7385031
AACGCAG36300.024.9916936
TACATGG20500.024.3278852
GAGTACT23850.024.09987312-13
TGGCGCG500.001652817423.75132284-85
AACGCGA500.001652817423.75132276-77
ACGCAGA39300.022.8447827
Produced by FastQC (version 0.11.5)