##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937275_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 444802 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25225156361707 33.0 31.0 34.0 31.0 34.0 2 32.45000696939312 34.0 31.0 34.0 31.0 34.0 3 32.51317664938557 34.0 31.0 34.0 31.0 34.0 4 36.138657200282374 37.0 35.0 37.0 35.0 37.0 5 36.07493671341406 37.0 35.0 37.0 35.0 37.0 6 36.11858759627879 37.0 35.0 37.0 35.0 37.0 7 36.0566027131173 37.0 35.0 37.0 35.0 37.0 8 36.04005827311928 37.0 35.0 37.0 35.0 37.0 9 37.74278892630878 39.0 37.0 39.0 35.0 39.0 10-11 37.666767460577965 39.0 37.0 39.0 35.0 39.0 12-13 37.568193263519504 39.0 37.0 39.0 35.0 39.0 14-15 38.78873858480852 40.0 38.0 41.0 35.0 41.0 16-17 38.74702451877465 40.0 38.0 41.0 35.0 41.0 18-19 38.766819843435954 40.0 38.0 41.0 35.0 41.0 20-21 38.67876942999357 40.0 38.0 41.0 34.0 41.0 22-23 38.621484390807595 40.0 38.0 41.0 34.0 41.0 24-25 38.65600874096789 40.0 38.0 41.0 34.0 41.0 26-27 38.49783049536648 40.0 38.0 41.0 34.0 41.0 28-29 38.354744358163856 40.0 38.0 41.0 34.0 41.0 30-31 38.352697379957824 40.0 38.0 41.0 34.0 41.0 32-33 38.21283739731386 40.0 38.0 41.0 34.0 41.0 34-35 38.08951173780694 40.0 37.0 41.0 34.0 41.0 36-37 37.91415843453942 40.0 37.0 41.0 33.0 41.0 38-39 37.78273254167023 40.0 37.0 41.0 33.0 41.0 40-41 37.57928134315942 40.0 37.0 41.0 33.0 41.0 42-43 37.35615622231914 40.0 37.0 41.0 33.0 41.0 44-45 37.007057072585106 40.0 36.0 41.0 31.5 41.0 46-47 36.57617996321959 40.0 36.0 41.0 30.5 41.0 48-49 36.17587263546477 39.0 36.0 41.0 30.0 41.0 50-51 35.93301738751175 39.0 35.5 41.0 29.0 41.0 52-53 35.69161559525362 39.0 35.0 40.0 28.0 41.0 54-55 35.480220412678 39.0 35.0 40.0 28.0 41.0 56-57 35.193563653041124 38.5 35.0 40.0 27.0 41.0 58-59 34.940730931965234 38.0 34.5 40.0 26.5 41.0 60-61 34.61273555424661 38.0 34.0 40.0 26.0 41.0 62-63 34.24258434089775 37.5 34.0 40.0 25.5 41.0 64-65 33.82753225030463 37.0 33.5 39.5 24.5 41.0 66-67 33.4111301208178 36.5 33.0 39.0 23.0 40.5 68-69 32.96406490978008 36.0 32.5 39.0 22.0 40.0 70-71 32.53479525721556 35.5 32.0 38.5 21.5 40.0 72-73 32.04835634731858 35.0 31.5 38.0 20.0 40.0 74-75 31.561382367885038 35.0 31.0 37.0 18.0 39.0 76-77 30.23373883210957 33.5 29.5 35.5 17.5 38.5 78-79 30.872002598909177 34.0 31.0 36.0 17.5 38.5 80-81 30.67797019797573 34.0 31.0 36.0 14.0 37.5 82-83 30.278581256379244 34.0 30.5 35.0 9.0 37.0 84-85 29.825677492457316 34.0 30.0 35.0 6.5 36.5 86-87 29.436429017855133 34.0 29.5 35.0 2.0 36.0 88-89 28.980199054860364 34.0 29.0 35.0 2.0 36.0 90-91 28.425454471877373 34.0 29.0 35.0 2.0 35.0 92-93 27.902990544107265 33.0 27.5 35.0 2.0 35.0 94-95 27.41040395501819 33.0 26.5 35.0 2.0 35.0 96-97 26.74462232633846 33.0 25.5 34.5 2.0 35.0 98-99 25.76486166878746 32.0 24.0 34.0 2.0 35.0 100-101 22.799396135808742 29.0 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22435826760795408 1101 2 0.2450792689066219 1101 3 0.23675188211256426 1101 4 0.20547202264756947 1101 5 0.11536047775351932 1101 6 0.1760745126336971 1101 7 0.20835489011723496 1101 8 0.10170202506161985 1101 9 0.14013528847696222 1101 10-11 0.13049462592314143 1101 12-13 0.22732941041736154 1101 14-15 0.3215545156087245 1101 16-17 0.2690691543025139 1101 18-19 0.2324431878933737 1101 20-21 0.3333368779527959 1101 22-23 0.3334544375608317 1101 24-25 0.25039812291927177 1101 26-27 0.21308202833446188 1101 28-29 0.154180211736751 1101 30-31 0.25670605547112757 1101 32-33 0.05786682650780506 1101 34-35 0.10763319796166826 1101 36-37 0.24809851222280344 1101 38-39 0.15510432414993858 1101 40-41 0.12196375613161337 1101 42-43 0.20335963144512448 1101 44-45 0.36112396289024673 1101 46-47 0.42801886765547437 1101 48-49 0.49980725001451987 1101 50-51 0.45724782533969943 1101 52-53 0.5818044005271688 1101 54-55 0.40223511860529726 1101 56-57 0.391241497654363 1101 58-59 0.4418292803086956 1101 60-61 0.23335395722553187 1101 62-63 0.23080709207358296 1101 64-65 0.11910644088802513 1101 66-67 0.22105278499585523 1101 68-69 0.5405809601958751 1101 70-71 0.5803505499647272 1101 72-73 0.5900776047336649 1101 74-75 0.8118496828264803 1101 76-77 0.7898539440948547 1101 78-79 0.8125851229061531 1101 80-81 0.6300383940276113 1101 82-83 0.5649051940975127 1101 84-85 0.36793851957533263 1101 86-87 0.5870883804351656 1101 88-89 0.7002045837343935 1101 90-91 0.6200129839944921 1101 92-93 0.4189541140779731 1101 94-95 0.6082852046167062 1101 96-97 1.1769019637244789 1101 98-99 1.4402717158420053 1101 100-101 2.8450528841229534 1102 1 0.24811101664256086 1102 2 0.2735302441155767 1102 3 0.15469371022398093 1102 4 0.10589905979462344 1102 5 0.019147673179560343 1102 6 0.08128425682019014 1102 7 0.03944766373304276 1102 8 0.041202824904651436 1102 9 0.1463051168172953 1102 10-11 0.18137527081191962 1102 12-13 0.1856340157145766 1102 14-15 0.21762125004176625 1102 16-17 0.2218089232609799 1102 18-19 0.14389497793390404 1102 20-21 0.33163908508353046 1102 22-23 0.12860605346176612 1102 24-25 0.14653571227141526 1102 26-27 0.22409315528194895 1102 28-29 0.1745537261679928 1102 30-31 0.32576234970435536 1102 32-33 0.43861254727946886 1102 34-35 0.4026540047811835 1102 36-37 0.49290907069582346 1102 38-39 0.405294406096381 1102 40-41 0.5312912611936511 1102 42-43 0.4607919036806294 1102 44-45 0.6924974309932708 1102 46-47 0.4679619657050509 1102 48-49 0.4364670401204549 1102 50-51 0.6270993297803997 1102 52-53 0.6558505161787451 1102 54-55 0.6199790979810729 1102 56-57 0.6082196988659874 1102 58-59 0.5218203961919201 1102 60-61 0.7836636749776886 1102 62-63 0.7500196759072111 1102 64-65 0.6193339941859151 1102 66-67 0.8001568517956841 1102 68-69 0.6390052558025943 1102 70-71 0.4165476029451298 1102 72-73 0.7159023151141852 1102 74-75 0.4441494427373165 1102 76-77 0.5863804179453034 1102 78-79 0.614518372121978 1102 80-81 0.546901047217311 1102 82-83 0.7397873977945046 1102 84-85 0.7463501158003965 1102 86-87 0.9102086130573177 1102 88-89 0.7475315819112396 1102 90-91 1.1526880024576478 1102 92-93 0.943072038464873 1102 94-95 0.8962164317421468 1102 96-97 1.434037190337584 1102 98-99 2.107155489247976 1102 100-101 2.791733194903525 1103 1 0.09786105508611342 1103 2 0.056328872072988645 1103 3 0.07684943643055675 1103 4 0.12298996546198993 1103 5 -0.07083534889174814 1103 6 -0.13055698542441263 1103 7 0.15388706257000706 1103 8 -0.14131147766651964 1103 9 0.133610898413977 1103 10-11 0.12094984973732181 1103 12-13 0.032820104483100465 1103 14-15 0.25447998802619054 1103 16-17 0.17552916088921933 1103 18-19 0.12786009315472313 1103 20-21 0.338882164459271 1103 22-23 0.102957141963536 1103 24-25 -0.12496051084556115 1103 26-27 0.03350256814689345 1103 28-29 0.07747041506392094 1103 30-31 -0.00863694230660883 1103 32-33 -0.00844518709283193 1103 34-35 -0.06182885880853917 1103 36-37 -0.032272241657267386 1103 38-39 0.022815565742256183 1103 40-41 -0.22832982658632517 1103 42-43 -0.2550255004223203 1103 44-45 -0.06832717539181488 1103 46-47 -0.06468681084844974 1103 48-49 -0.09686587477964537 1103 50-51 -0.2713328008711997 1103 52-53 -0.3500603763707346 1103 54-55 -0.2217401605133844 1103 56-57 -0.45492501522380735 1103 58-59 -0.46463962721941954 1103 60-61 -0.38636667189301477 1103 62-63 -0.4612158842232077 1103 64-65 -0.1425011284725599 1103 66-67 -0.10195644528040759 1103 68-69 -0.17325525654798213 1103 70-71 -0.16735819936398855 1103 72-73 -0.3784946545922949 1103 74-75 -0.1608689317123151 1103 76-77 -0.32482281821737047 1103 78-79 -0.26141878757411163 1103 80-81 -0.25853220378928654 1103 82-83 -0.4437138841803332 1103 84-85 -0.5178653689492805 1103 86-87 -0.5425701497820477 1103 88-89 -0.26658897079722266 1103 90-91 0.06657914914255869 1103 92-93 0.06334073536961782 1103 94-95 0.0817277623912318 1103 96-97 0.46792714448776707 1103 98-99 0.8171420261584963 1103 100-101 1.6872169403412975 1104 1 0.09323457823471415 1104 2 0.05916817406590269 1104 3 0.06678576832622696 1104 4 -0.04652501113538676 1104 5 0.02663053804782578 1104 6 0.015573384657706413 1104 7 -0.14730955188665718 1104 8 -0.048151384992856094 1104 9 -0.0745337186225612 1104 10-11 -0.10706087331183056 1104 12-13 -0.1351545147513704 1104 14-15 -0.25056085396376915 1104 16-17 -0.20553183005600317 1104 18-19 -0.022174317167653612 1104 20-21 -0.1588658510920382 1104 22-23 -0.09047210789143634 1104 24-25 -0.14727677593835864 1104 26-27 -0.14344756030630634 1104 28-29 -0.2464045835848836 1104 30-31 -0.16600021081882232 1104 32-33 0.19010597619224967 1104 34-35 -0.006608398538944016 1104 36-37 0.07107042389962004 1104 38-39 0.045645283289353245 1104 40-41 0.191355400035377 1104 42-43 -0.029524388842794735 1104 44-45 0.1930633619496973 1104 46-47 0.06395014403619115 1104 48-49 0.23046817198235914 1104 50-51 0.2610363228005852 1104 52-53 0.5333264641131024 1104 54-55 0.471013494006975 1104 56-57 0.33465496265269223 1104 58-59 0.33528326483305193 1104 60-61 0.11476472509401958 1104 62-63 0.3820387917972994 1104 64-65 -0.11408108473001022 1104 66-67 0.2773624104953072 1104 68-69 0.28532984864671107 1104 70-71 0.06941184239681064 1104 72-73 0.2597934048456736 1104 74-75 -0.052517852741527093 1104 76-77 0.17925597601610832 1104 78-79 0.24638028496861253 1104 80-81 0.17238051649983532 1104 82-83 0.03271954896095153 1104 84-85 -0.0037913669387066307 1104 86-87 0.078887478680155 1104 88-89 0.06938909307265817 1104 90-91 0.43247797919159936 1104 92-93 0.5369222554050701 1104 94-95 0.7126347176001637 1104 96-97 0.7286042531559218 1104 98-99 1.116650553072411 1104 100-101 1.7416483052600675 1105 1 0.07242936006107215 1105 2 0.10351515262340882 1105 3 0.20781140935186926 1105 4 0.1429711652028942 1105 5 0.26890824085184306 1105 6 0.21837740325119626 1105 7 -0.052730154675678875 1105 8 0.19337658021932214 1105 9 0.0890590239175495 1105 10-11 0.14260174477183796 1105 12-13 0.13446986671511496 1105 14-15 0.2151199077696262 1105 16-17 0.3219460526850355 1105 18-19 0.09358516701288266 1105 20-21 0.08654200615792007 1105 22-23 0.37121630116539706 1105 24-25 -0.006082443954554151 1105 26-27 -0.20605064922518324 1105 28-29 -0.07872577116923196 1105 30-31 -0.15634733932384393 1105 32-33 0.0502424284339682 1105 34-35 0.03680140802875087 1105 36-37 0.0683901120087782 1105 38-39 -0.0026003307457287406 1105 40-41 -0.057519993576860884 1105 42-43 -0.3727917452680458 1105 44-45 0.10843673127043019 1105 46-47 0.21601567110172226 1105 48-49 0.6281289906958136 1105 50-51 0.49382984506778627 1105 52-53 0.3183579530919616 1105 54-55 0.5080317876042173 1105 56-57 0.3225841874240274 1105 58-59 0.35398921774953607 1105 60-61 0.383808059926821 1105 62-63 0.5143674767575632 1105 64-65 0.449440432738875 1105 66-67 0.42686008630877126 1105 68-69 0.44556751546332407 1105 70-71 0.5291696771546412 1105 72-73 0.5381147423775445 1105 74-75 0.8297171150197578 1105 76-77 0.3638788580074106 1105 78-79 0.4819853232052296 1105 80-81 0.5716532710357463 1105 82-83 0.49523266936880717 1105 84-85 0.6302617144827956 1105 86-87 0.9800158337558784 1105 88-89 1.1831242857552198 1105 90-91 1.364926622166557 1105 92-93 1.1668705235112355 1105 94-95 1.0462679459702358 1105 96-97 1.6957657466332066 1105 98-99 1.4925689816224157 1105 100-101 2.3201458311260126 1106 1 -0.024841180676801855 1106 2 0.19131526429286794 1106 3 0.03133071528323228 1106 4 0.15301067901125265 1106 5 0.13770736467608913 1106 6 0.0998789279688026 1106 7 0.10870737485230109 1106 8 -0.06690989489127475 1106 9 -0.04301158553999329 1106 10-11 -0.22086086743448874 1106 12-13 -0.08427879110389114 1106 14-15 0.12034582367393654 1106 16-17 -0.11263067711782071 1106 18-19 0.05823748844912302 1106 20-21 0.2956484357417395 1106 22-23 0.35037491474611926 1106 24-25 0.19228653770588977 1106 26-27 0.389534112188727 1106 28-29 0.5674967946513263 1106 30-31 0.5285568826406788 1106 32-33 0.522604237479193 1106 34-35 0.2641239180145831 1106 36-37 0.3902075324442933 1106 38-39 0.48860569940502074 1106 40-41 0.5001508392196072 1106 42-43 0.44118196945943566 1106 44-45 0.3241574287750453 1106 46-47 0.7703525263179714 1106 48-49 0.9498766177198164 1106 50-51 0.7433896097029233 1106 52-53 1.007385355059057 1106 54-55 0.7254704767765716 1106 56-57 0.9238705336852462 1106 58-59 0.9751076303252333 1106 60-61 0.8294443610907649 1106 62-63 1.1067417412423737 1106 64-65 1.115607808077101 1106 66-67 0.9576228746092497 1106 68-69 0.8154242779080292 1106 70-71 1.0044574407589906 1106 72-73 1.0714491494556668 1106 74-75 0.9435394317315264 1106 76-77 0.562220996048044 1106 78-79 0.9111074294630122 1106 80-81 0.8317249971878766 1106 82-83 0.8790320079420155 1106 84-85 1.3976456426707458 1106 86-87 1.5204691688072032 1106 88-89 1.6375760316642527 1106 90-91 1.5122873574669917 1106 92-93 1.8260800325951188 1106 94-95 1.0422628778948138 1106 96-97 1.316411711500276 1106 98-99 1.3296282093851666 1106 100-101 1.9353801222396996 1107 1 -0.002447890348094006 1107 2 0.013089620708512939 1107 3 -0.0041362174893748715 1107 4 0.041816409792815534 1107 5 0.054460159382486495 1107 6 0.04352743052888286 1107 7 0.15180703390406336 1107 8 0.15059607467285474 1107 9 -0.04587460037777191 1107 10-11 -0.10544335433709051 1107 12-13 0.15295049955745554 1107 14-15 -0.014444558496968796 1107 16-17 -0.05607722427441075 1107 18-19 0.18787503789850035 1107 20-21 0.19385697251544087 1107 22-23 0.08716465541161966 1107 24-25 0.1143258120919981 1107 26-27 0.03362929258242531 1107 28-29 0.13408564908899478 1107 30-31 -0.0701725802767399 1107 32-33 0.02010058446233387 1107 34-35 0.0031589275777577086 1107 36-37 -0.10152714567443155 1107 38-39 -0.10721025982374499 1107 40-41 -0.4716407428039915 1107 42-43 -0.3481963642896915 1107 44-45 -0.11830904068810355 1107 46-47 -0.6113847653412776 1107 48-49 -0.7678520967746394 1107 50-51 -0.9082432591329805 1107 52-53 -0.8359655602741114 1107 54-55 -0.40570252772584325 1107 56-57 -0.46983261563107703 1107 58-59 -0.6126865414794196 1107 60-61 -0.5121275647322108 1107 62-63 -0.4894513994017444 1107 64-65 -0.4735228333094952 1107 66-67 -0.32800443451319694 1107 68-69 -0.14378964038319708 1107 70-71 -0.6059230669806546 1107 72-73 -0.5085073707339305 1107 74-75 -0.3001019429907892 1107 76-77 -0.38739410071235625 1107 78-79 -0.12944402758582285 1107 80-81 -0.08242019686750979 1107 82-83 -0.35046654867083404 1107 84-85 -0.6102365579303708 1107 86-87 -0.44958500275985 1107 88-89 -0.07255856567231334 1107 90-91 -0.3127420448638141 1107 92-93 -0.2925539172961855 1107 94-95 -0.41790735863060036 1107 96-97 -0.023728706903803243 1107 98-99 0.12813908527762763 1107 100-101 1.0406404665142652 1108 1 -0.2309316932999792 1108 2 -0.16600436689611087 1108 3 -0.3296106280701707 1108 4 -0.15242038018865145 1108 5 -0.10086537892178171 1108 6 -0.1686634837896932 1108 7 -0.11712146906699417 1108 8 -0.1137959769532344 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1.2163442009521503 2316 96-97 1.5063032175480373 2316 98-99 1.2781680785422687 2316 100-101 1.6444714007345986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 4.0 5 5.0 6 9.0 7 34.0 8 86.0 9 196.0 10 326.0 11 620.0 12 973.0 13 1451.0 14 1893.0 15 2633.0 16 3859.0 17 5786.0 18 4920.0 19 2705.0 20 2192.0 21 2315.0 22 2353.0 23 2546.0 24 3035.0 25 3452.0 26 4191.0 27 5050.0 28 6285.0 29 7882.0 30 9991.0 31 12609.0 32 16116.0 33 21572.0 34 30023.0 35 43664.0 36 67397.0 37 99895.0 38 73320.0 39 5411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53810859380447 20.828544502947523 16.335853139036146 21.29749376421186 2 15.242737217908193 21.430883853939505 40.50656247049249 22.819816457659815 3 18.459674192112445 25.894892558936334 31.477151631512445 24.16828161743877 4 12.219144698090387 18.251266855814496 38.78984357084725 30.739744875247865 5 12.687275179856114 38.482239208633096 34.797661870503596 14.032823741007194 6 29.744020935157668 38.542317705406 17.746997540478684 13.966663818957647 7 27.00437498032833 34.30335295254967 21.370857145426505 17.321414921695496 8 26.019217539489482 35.69655711979712 19.9700091276568 18.314216213056596 9 26.181087315254874 16.78612056600465 21.056784816615036 35.97600730212544 10-11 23.94986982972199 27.82631373060373 27.945243051964695 20.278573387709585 12-13 25.296859911799192 25.302143416808963 28.588708362889108 20.812288308502733 14-15 22.334207130363623 26.42085692060737 27.488073344993953 23.756862604035053 16-17 20.71865684057176 29.868345915710808 28.020332642389196 21.39266460132823 18-19 20.612654619358725 27.63746565887743 31.632389242854124 20.117490478909716 20-21 21.334259590243725 26.649895121638668 31.97160078327162 20.044244504845988 22-23 21.13736510791367 26.58082284172662 31.85611510791367 20.42569694244604 24-25 20.813530514700922 26.75403887572448 32.04009874056322 20.39233186901138 26-27 20.7512916091494 27.027680229499502 31.84193132101602 20.379096840335077 28-29 20.509701780280334 26.96871216609205 32.17061104185238 20.350975011775233 30-31 20.708047765234078 26.874857379881544 31.914644880120413 20.502449974763966 32-33 20.66566921574953 26.978724169599626 31.71275661785452 20.64284999679632 34-35 20.93335911259392 26.912873593194277 31.609570100853862 20.544197193357945 36-37 21.164248362192616 26.69884577857114 31.542461589651126 20.594444269585118 38-39 20.782304016510736 27.070614644959605 31.648162157726535 20.49891918080312 40-41 20.897388051312717 27.019999910072347 31.69252836093363 20.390083677681307 42-43 20.77320862468242 26.888616588349034 31.513141623760593 20.82503316320795 44-45 21.020813755333833 27.099136244890985 31.040440465645386 20.839609534129792 46-47 21.038462057274923 27.603293150660296 30.468950229540336 20.889294562524448 48-49 21.07735576728522 27.86048623882087 30.090354809555713 20.9718031843382 50-51 21.302849357691738 27.74706498621859 30.003462214648312 20.94662344144136 52-53 21.464044676058112 27.981214113245894 29.54797865117513 21.006762559520865 54-55 21.202242795670884 28.112283667789264 29.603958615293998 21.08151492124586 56-57 21.19830846084325 28.324288110215335 29.474462794681678 21.002940634259737 58-59 21.188416418990922 27.82035602357903 29.807307521099276 21.183920036330772 60-61 21.223937842006105 28.059001533266485 29.88262193065679 20.83443869407062 62-63 21.15334463424175 28.009653733571344 30.03920845679651 20.797793175390396 64-65 21.07645649075319 27.843399984712295 29.970638621229224 21.10950490330529 66-67 20.892442030386555 28.20513396972136 29.655554606319217 21.24686939357287 68-69 20.97629956699835 27.9334400474818 29.80281113843913 21.287449247080723 70-71 20.995296783737484 27.988408325502135 29.768751039788487 21.24754385097189 72-73 21.048922042403376 27.906955830660777 29.651449698122413 21.39267242881343 74-75 21.055885540083004 28.288317048934132 29.196249117584905 21.45954829339796 76-77 21.053187710486913 28.01201433446792 29.65443051065418 21.280367444390986 78-79 21.027783148457065 28.052144549709755 29.429948606346194 21.490123695486982 80-81 20.819825450425135 28.294949213357857 29.28673881862042 21.598486517596594 82-83 20.973536480358632 28.25094817682514 29.341211013464445 21.43430432935178 84-85 21.006898582851 28.125916841557412 29.157500592961128 21.709683982630455 86-87 20.75822500798108 28.021793966753744 29.221316450915243 21.998664574349934 88-89 20.917959001982904 27.71840054676013 29.343056011438794 22.020584439818165 90-91 20.958277030445156 27.815687764604842 29.46587763930328 21.760157565646733 92-93 20.633907153150762 27.65115322270609 29.471196973001167 22.243742651141982 94-95 20.57376090934843 27.608239171586458 29.475362071213706 22.342637847851403 96-97 20.896263955647683 27.657699380848104 29.138245781268967 22.307790882235242 98-99 20.48803847113653 27.623026926648098 29.14842817349781 22.74050642871756 100-101 20.30673136160664 27.426397514131406 29.57129109575181 22.695580028510143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 126.0 2 94.5 3 116.5 4 101.0 5 100.0 6 188.0 7 405.5 8 692.0 9 884.0 10 860.0 11 783.5 12 804.5 13 847.0 14 894.0 15 952.0 16 978.0 17 989.0 18 1034.5 19 1067.5 20 1169.0 21 1333.5 22 1534.5 23 1773.0 24 2067.0 25 2515.0 26 3195.0 27 3987.5 28 4613.5 29 5453.5 30 6470.0 31 7455.0 32 8581.5 33 9821.0 34 11140.0 35 12479.0 36 14247.5 37 15602.0 38 16046.5 39 15961.0 40 15712.5 41 15942.5 42 15953.0 43 16159.0 44 16648.5 45 16762.5 46 16384.5 47 16211.5 48 16726.0 49 16106.5 50 14778.0 51 13659.0 52 12609.5 53 12106.0 54 11416.0 55 10103.5 56 8768.5 57 7738.0 58 6995.0 59 6184.0 60 5061.5 61 4039.0 62 3107.5 63 2235.0 64 1663.5 65 1218.5 66 834.0 67 615.0 68 449.5 69 304.0 70 230.0 71 174.5 72 121.5 73 94.0 74 80.5 75 62.5 76 34.0 77 20.5 78 19.5 79 7.5 80 4.0 81 5.0 82 2.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04294045440443164 2 0.0 3 0.0 4 0.0 5 4.4963826601499094E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004833611359661153 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2481913300749547E-4 22-23 4.4963826601499094E-4 24-25 0.0 26-27 0.0017985530640599638 28-29 0.0028102391625936932 30-31 3.372286995112432E-4 32-33 3.372286995112432E-4 34-35 0.0 36-37 0.0 38-39 1.1240956650374773E-4 40-41 0.0 42-43 0.007194212256239855 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.620478325187386E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.2481913300749547E-4 84-85 1.1240956650374773E-4 86-87 0.0 88-89 0.0 90-91 0.007868669655262341 92-93 0.0011240956650374772 94-95 0.0 96-97 0.0 98-99 2.2481913300749547E-4 100-101 0.010791318384359783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 444802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.55920548030998 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9600390154282 52.72874166436134 2 9.838336965350072 12.50633761530429 3 2.8284019410722774 5.393129404605614 4 1.2819053863723078 3.2590755143501466 5 0.7361878892451238 2.339575866232325 6 0.47543694863836966 1.8131036826862634 7 0.3415317231781553 1.5195239480067302 8 0.2422680278615199 1.2318690691327845 9 0.18112652885759983 1.0361032439035933 >10 1.0342540401397735 11.973422892727765 >50 0.058068053204018616 2.5219433959061464 >100 0.021018497754043675 1.8999485554219517 >500 3.5624572464480807E-4 0.13449760957223683 >1k 0.0010687371739344243 1.6427275377888522 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.6591696979779768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2570 0.5777851718292634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1753 0.39410794016213957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.1335425650064523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1240956650374773E-4 0.0 22-23 0.0 0.0 0.0 2.2481913300749547E-4 0.0 24-25 0.0 0.0 0.0 2.2481913300749547E-4 0.0 26-27 0.0 0.0 0.0 6.744573990224864E-4 0.0 28-29 0.0 0.0 0.0 0.0013489147980449729 0.0 30-31 0.0 0.0 0.0 0.002922648729097441 0.0 32-33 0.0 0.0 0.0 0.004946020926164901 0.0 34-35 0.0 0.0 0.0 0.006294935724209872 0.0 36-37 0.0 0.0 0.0 0.007419031389247351 0.0 38-39 0.0 0.0 0.0 0.010116860985337296 0.0 40-41 0.0 0.0 0.0 0.013489147980449728 0.0 42-43 0.0 0.0 0.0 0.0223695037342458 0.0 44-45 0.0 0.0 0.0 0.03687033781322926 0.0 46-47 0.0 0.0 0.0 0.04979743796116025 0.0 48-49 0.0 0.0 0.0 0.08509404184333703 0.0 50-51 0.0 0.0 0.0 0.1260111240507012 0.0 52-53 0.0 0.0 0.0 0.1606332705338555 0.0 54-55 0.0 0.0 0.0 0.2129037189580982 0.0 56-57 0.0 0.0 0.0 0.2747289805351595 0.0 58-59 0.0 0.0 0.0 0.35881133627996276 0.0 60-61 0.0 0.0 0.0 0.4539098295421333 0.0 62-63 0.0 0.0 0.0 0.5890261284796381 0.0 64-65 0.0 0.0 0.0 0.7145876142643244 0.0 66-67 0.0 0.0 0.0 0.8879231658131034 0.0 68-69 0.0 0.0 0.0 1.1164518145152225 0.0 70-71 0.0 0.0 0.0 1.2780967711476117 0.0 72-73 0.0 0.0 0.0 1.5031407232881149 0.0 74-75 0.0 0.0 0.0 1.8025998084540986 0.0 76-77 0.0 0.0 0.0 2.05068772172787 0.0 78-79 0.0 0.0 0.0 2.280902513927545 0.0 80-81 0.0 0.0 0.0 2.611948687281082 0.0 82-83 0.0 0.0 0.0 3.0186464988916417 0.0 84-85 0.0 0.0 0.0 3.410281428590699 0.0 86-87 0.0 0.0 0.0 3.9119653238969248 0.0 88-89 0.0 0.0 0.0 4.528981434435996 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1715 0.0 46.261417 1 GTATCAA 2755 0.0 33.109013 1 ATAGCCC 100 4.9482915E-5 28.498383 3 GTATAGG 170 3.621608E-9 27.945871 1 CTCGCCA 85 6.403085E-4 27.939589 4 TAACGTA 35 0.008388291 27.144367 86-87 CCTAGAC 140 5.7955185E-7 27.141315 3 ATCAACG 3405 0.0 26.503633 3 ACGCGAA 45 8.943046E-4 26.390356 78-79 TAACGCG 45 8.943046E-4 26.390356 76-77 TCAACGC 3450 0.0 26.157934 4 TATCAAC 3505 0.0 25.747467 2 CAACGCA 3505 0.0 25.747467 5 GTACATG 1975 0.0 25.738503 1 AACGCAG 3630 0.0 24.991693 6 TACATGG 2050 0.0 24.327885 2 GAGTACT 2385 0.0 24.099873 12-13 TGGCGCG 50 0.0016528174 23.751322 84-85 AACGCGA 50 0.0016528174 23.751322 76-77 ACGCAGA 3930 0.0 22.844782 7 >>END_MODULE