FastQCFastQC Report
Thu 26 May 2016
SRR937274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412774
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.5387936255675018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18380.44527998371990485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.31470005378245725No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5300.12839956004980932No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.10611133453172923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.044.200641
GTATCAA23700.034.9024471
TATCAAC29300.027.7381782
ATCAACG29550.027.1818313
GGACCGA700.00838616527.142376
TCAACGC29600.027.1359164
CAACGCA29750.026.9990965
GTACCTG3000.026.9390741
AACGCAG30600.026.233196
GAGTACT18700.025.01960612-13
TACCTGG3300.024.4841182
GTACATG20050.024.421811
GTACTTT19650.023.81000914-15
GTCTTAG1201.6986087E-423.769771
CATGGGT2801.8189894E-1223.7639964
GGGTATG1401.7761346E-523.7495737
ACGCAGA33950.023.2249167
TACATGG21100.023.2008692
ATGGGTA1652.411216E-623.0438755
CGCAGAG34250.023.0214868
Produced by FastQC (version 0.11.5)