##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937274_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 412774 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8159404419852 33.0 31.0 34.0 30.0 34.0 2 31.925748714793084 33.0 31.0 34.0 30.0 34.0 3 31.94463071802003 33.0 31.0 34.0 30.0 34.0 4 35.56897963534525 37.0 35.0 37.0 33.0 37.0 5 35.590238241749724 37.0 35.0 37.0 33.0 37.0 6 35.63255679863558 37.0 35.0 37.0 33.0 37.0 7 35.58336280870404 37.0 35.0 37.0 33.0 37.0 8 35.577957914015904 37.0 35.0 37.0 33.0 37.0 9 37.2074961116737 39.0 37.0 39.0 34.0 39.0 10-11 37.10545358961562 39.0 37.0 39.0 34.0 39.0 12-13 36.98927015751961 39.0 37.0 39.0 33.0 39.0 14-15 38.15924331474366 40.0 38.0 41.0 33.0 41.0 16-17 38.131638620649554 40.0 38.0 41.0 33.0 41.0 18-19 38.13902038403582 40.0 38.0 41.0 33.5 41.0 20-21 38.08954173470228 40.0 38.0 41.0 33.0 41.0 22-23 37.94368589106872 40.0 38.0 41.0 33.0 41.0 24-25 37.801439770915806 40.0 38.0 41.0 33.0 41.0 26-27 37.65010514228125 40.0 37.0 41.0 32.5 41.0 28-29 37.70280952773189 40.0 37.0 41.0 33.0 41.0 30-31 37.535108800457394 40.0 37.0 41.0 32.5 41.0 32-33 37.33058647104711 40.0 36.5 41.0 32.0 41.0 34-35 37.21631328523599 40.0 36.5 41.0 32.0 41.0 36-37 37.221938639546096 40.0 37.0 41.0 32.0 41.0 38-39 37.129677499067284 40.0 37.0 41.0 31.0 41.0 40-41 36.95750580220653 40.0 36.0 41.0 31.0 41.0 42-43 36.71349697413112 40.0 36.0 41.0 31.0 41.0 44-45 36.476502880510886 39.5 36.0 41.0 30.0 41.0 46-47 36.10893733616943 39.0 36.0 41.0 29.5 41.0 48-49 35.768048617403224 39.0 35.0 41.0 28.0 41.0 50-51 34.88437619617515 38.5 34.0 40.0 26.0 40.5 52-53 34.96918652822126 38.5 35.0 40.0 26.5 40.5 54-55 35.303078682281345 39.0 35.0 40.0 27.0 41.0 56-57 35.11750982377766 39.0 35.0 40.0 26.0 41.0 58-59 34.912753710262756 38.5 35.0 40.0 26.0 41.0 60-61 34.64971509833468 38.0 34.0 40.0 25.5 41.0 62-63 34.34099531462738 38.0 34.0 40.0 24.5 41.0 64-65 33.97378347473436 37.5 34.0 40.0 23.0 41.0 66-67 33.61659649105806 37.0 33.5 40.0 22.0 41.0 68-69 33.16864918817561 36.0 33.0 39.0 21.5 41.0 70-71 32.74438797017254 36.0 32.5 39.0 20.0 40.0 72-73 32.35549477438017 35.5 32.0 38.5 18.5 40.0 74-75 31.908184624031552 35.0 32.0 37.5 17.5 39.5 76-77 31.4649747803883 35.0 31.0 37.0 12.5 39.0 78-79 30.99877778154632 35.0 31.0 36.5 8.0 39.0 80-81 30.51349891223769 34.5 31.0 36.0 7.0 38.5 82-83 30.04519906777074 34.0 30.0 35.5 2.0 37.0 84-85 29.611596176115743 34.0 30.0 35.0 2.0 37.0 86-87 29.178377271824292 34.0 29.0 35.0 2.0 36.0 88-89 28.790484623547023 34.0 29.0 35.0 2.0 36.0 90-91 28.382914137033826 34.0 29.0 35.0 2.0 35.5 92-93 27.947142988657234 34.0 28.0 35.0 2.0 35.0 94-95 27.460937462146354 33.5 27.0 35.0 2.0 35.0 96-97 26.86942854927878 33.0 25.5 35.0 2.0 35.0 98-99 26.140910037938436 33.0 24.5 35.0 2.0 35.0 100-101 24.251956276315852 31.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23631746938710307 1101 2 0.10793692106219055 1101 3 0.20489206647835445 1101 4 0.057688276438398134 1101 5 0.12105990256223009 1101 6 0.11963842555186943 1101 7 0.11702623934424139 1101 8 0.11174247667020154 1101 9 0.40448314333219315 1101 10-11 0.17866693351825802 1101 12-13 0.2100197231866261 1101 14-15 0.3272097240660017 1101 16-17 0.32086055398549007 1101 18-19 0.2131908650028791 1101 20-21 0.3005194771002948 1101 22-23 0.3409385617979268 1101 24-25 0.4213798831572788 1101 26-27 0.37017756419537307 1101 28-29 0.15950385044735071 1101 30-31 0.6093792383082572 1101 32-33 0.2913056288445972 1101 34-35 0.36679728016542157 1101 36-37 0.5893337802619314 1101 38-39 0.4759866638203434 1101 40-41 0.47251509156810556 1101 42-43 0.32562688104913917 1101 44-45 0.8431737195575977 1101 46-47 0.6718926990276373 1101 48-49 0.7546407574408747 1101 50-51 0.5398520387415431 1101 52-53 0.5074984770213931 1101 54-55 0.7065120422243183 1101 56-57 0.5100185136798672 1101 58-59 0.7156306151496494 1101 60-61 0.524142021960877 1101 62-63 0.286449136345432 1101 64-65 0.2881438484919201 1101 66-67 0.1537394209781695 1101 68-69 0.5522679820744969 1101 70-71 0.5871132783016293 1101 72-73 0.23786778096663141 1101 74-75 0.09055059237227425 1101 76-77 0.036267760308394514 1101 78-79 0.20673011944952435 1101 80-81 0.2753257555744568 1101 82-83 0.06061479128121405 1101 84-85 0.06028937815972313 1101 86-87 0.16886441105467043 1101 88-89 0.2706602429558309 1101 90-91 0.36575299569907926 1101 92-93 0.621308495189627 1101 94-95 0.6530966830942049 1101 96-97 0.4714296362067465 1101 98-99 0.7558291511488946 1101 100-101 1.4807924183415757 1102 1 0.008610833217524316 1102 2 0.048763489223890844 1102 3 0.3196271856447872 1102 4 0.18803984618521952 1102 5 0.11498944771255282 1102 6 0.016226994310045484 1102 7 0.015228116605982223 1102 8 0.11385832959846454 1102 9 -0.05133639155589975 1102 10-11 0.15358433071387623 1102 12-13 0.13592494894413676 1102 14-15 0.42314756824435307 1102 16-17 0.44486339077831616 1102 18-19 0.25803121321450107 1102 20-21 0.2340485835810071 1102 22-23 0.2559674581023472 1102 24-25 0.4484198260456935 1102 26-27 0.3003438500398232 1102 28-29 0.4836966678194159 1102 30-31 0.3201977986358955 1102 32-33 0.4131057241871332 1102 34-35 0.3399217209673324 1102 36-37 0.49164994645853 1102 38-39 0.6725555065356801 1102 40-41 0.6254811604260837 1102 42-43 0.7432788713833816 1102 44-45 0.45980159950114086 1102 46-47 0.6053196527865055 1102 48-49 0.3730968188546271 1102 50-51 0.23296999812938424 1102 52-53 0.024380682017245192 1102 54-55 0.027659064648602794 1102 56-57 0.2151857975681537 1102 58-59 0.27519269699864424 1102 60-61 0.49227154691549657 1102 62-63 0.1956154057515178 1102 64-65 0.5700477873599752 1102 66-67 0.39802767482416357 1102 68-69 0.48921861765229124 1102 70-71 0.4660270778427673 1102 72-73 0.6306555639707696 1102 74-75 0.7181393419525044 1102 76-77 0.6475015068710128 1102 78-79 0.4363119038579697 1102 80-81 0.4196182364296277 1102 82-83 0.34037352299059265 1102 84-85 0.23838853909840196 1102 86-87 0.3330120933620542 1102 88-89 0.44792222124309333 1102 90-91 0.4659272424108174 1102 92-93 0.4622661006358939 1102 94-95 0.5271059778177225 1102 96-97 0.7256901092090722 1102 98-99 0.9894723868774555 1102 100-101 1.1955374005294281 1103 1 -0.005388309470635733 1103 2 0.19066487665830323 1103 3 0.05590612670188477 1103 4 -0.23436258033284219 1103 5 0.09871680084363987 1103 6 0.026238009139369467 1103 7 -0.09713128153940431 1103 8 -0.0532849228690182 1103 9 -0.014233237407971444 1103 10-11 -0.015755105088125276 1103 12-13 0.04075383926790721 1103 14-15 -0.14289528649555194 1103 16-17 -0.1365378257363261 1103 18-19 0.13003943906733895 1103 20-21 -0.058632523544993376 1103 22-23 -0.3785993009876023 1103 24-25 -0.03146997140081709 1103 26-27 0.045422446001779804 1103 28-29 -0.07313008465078497 1103 30-31 -0.13263784166210257 1103 32-33 -0.06655075421457468 1103 34-35 -0.2654136887246281 1103 36-37 -0.06611008219087466 1103 38-39 -0.26843512272035497 1103 40-41 0.13881290212562902 1103 42-43 -0.13384473887614234 1103 44-45 -0.14020002830614686 1103 46-47 -0.14976453198047324 1103 48-49 -0.23914456326228617 1103 50-51 -0.13755556658409773 1103 52-53 0.11398740377615013 1103 54-55 0.05467523962703069 1103 56-57 0.017362447215191423 1103 58-59 0.1370176317506946 1103 60-61 0.016045672376030495 1103 62-63 -0.26073629829141254 1103 64-65 -0.34738304439529344 1103 66-67 -0.27711896348248644 1103 68-69 -0.05870267243924587 1103 70-71 -0.09737737258236479 1103 72-73 0.13635967201376786 1103 74-75 0.09064133601643576 1103 76-77 0.29131285485823 1103 78-79 0.36203912676447203 1103 80-81 0.14163894213834993 1103 82-83 0.38995246350508594 1103 84-85 -0.0047448271528161 1103 86-87 0.1884587365521213 1103 88-89 0.48049735811455463 1103 90-91 0.49314824980649163 1103 92-93 0.4214746064715804 1103 94-95 0.31270341022302617 1103 96-97 0.2459644489975652 1103 98-99 0.6796952336953623 1103 100-101 0.6594502694312574 1104 1 -0.055891842614894216 1104 2 -0.11077602128910868 1104 3 -0.08558788858437083 1104 4 -0.044239761508634956 1104 5 -0.2920561645940367 1104 6 -0.2793489254373682 1104 7 -0.11560217516051097 1104 8 -0.05125396579552444 1104 9 0.18483660828735538 1104 10-11 0.00706011387068628 1104 12-13 -0.030591038874668186 1104 14-15 -0.03135488366903161 1104 16-17 -0.05013201888154839 1104 18-19 -0.06645265341931861 1104 20-21 -0.14636325949653894 1104 22-23 -0.0756622246043861 1104 24-25 0.022296312308263566 1104 26-27 0.01636413710401996 1104 28-29 -0.051507602316505086 1104 30-31 -0.1386976647645355 1104 32-33 -0.08573969330990394 1104 34-35 -0.19988828373496403 1104 36-37 0.042227657558207454 1104 38-39 -0.04017177128066152 1104 40-41 -0.23121485829354782 1104 42-43 -0.3741736577820731 1104 44-45 -0.3456421520382946 1104 46-47 -0.04609859437532293 1104 48-49 0.16554766862734738 1104 50-51 0.2231105633010131 1104 52-53 0.2355083382567571 1104 54-55 0.45589441474600534 1104 56-57 0.4802163625158755 1104 58-59 0.42851705491082015 1104 60-61 0.3855867241474087 1104 62-63 0.1833222509562944 1104 64-65 0.31380464488029247 1104 66-67 0.42378955932672824 1104 68-69 0.41236820676087405 1104 70-71 0.15932238226069018 1104 72-73 0.35391543320205443 1104 74-75 0.38999719815170764 1104 76-77 0.45540276977434857 1104 78-79 0.4120138900197716 1104 80-81 0.6349331785465608 1104 82-83 0.6773268806564587 1104 84-85 0.6086372571619485 1104 86-87 0.6453399556070707 1104 88-89 0.5625197827648449 1104 90-91 0.3480940508928718 1104 92-93 0.37429391137952805 1104 94-95 0.43995669099983914 1104 96-97 0.4941648287504883 1104 98-99 0.39248605713429185 1104 100-101 1.3857818081516342 1105 1 0.20132034945645216 1105 2 0.2557814315359508 1105 3 0.17097853699614163 1105 4 0.027467509142297786 1105 5 0.12282501437164939 1105 6 0.21381954484377275 1105 7 0.2197477623020987 1105 8 0.2682984840386169 1105 9 0.29195227526218304 1105 10-11 0.284461005708188 1105 12-13 0.2729905860097759 1105 14-15 0.2780760261139932 1105 16-17 0.2679385667946761 1105 18-19 0.23736348666442808 1105 20-21 0.3219432008873895 1105 22-23 0.2722631655745289 1105 24-25 0.12733193142838672 1105 26-27 0.21494752934938788 1105 28-29 0.13291311115350624 1105 30-31 0.2501319344523836 1105 32-33 0.10524744574061629 1105 34-35 0.07004890697687216 1105 36-37 -0.04332620616467864 1105 38-39 0.2597119907509935 1105 40-41 0.18504282292892782 1105 42-43 0.22925434265297184 1105 44-45 0.19935417728901683 1105 46-47 0.009930145632289111 1105 48-49 0.30802754790300213 1105 50-51 0.27561137900605814 1105 52-53 0.040051815335438334 1105 54-55 0.2421151173399494 1105 56-57 0.1963917934800321 1105 58-59 0.12756607878378645 1105 60-61 0.03729766547102997 1105 62-63 -0.05574730149353968 1105 64-65 0.13682044236792024 1105 66-67 0.23262908295497908 1105 68-69 -0.021739819776733782 1105 70-71 0.18530938536036956 1105 72-73 0.14428263642137296 1105 74-75 0.17958608281101007 1105 76-77 0.03496460021646186 1105 78-79 0.10755878098719762 1105 80-81 -0.22108128662283377 1105 82-83 -0.2169169607704795 1105 84-85 0.044940889768401604 1105 86-87 0.24085901591485737 1105 88-89 0.3934139314406835 1105 90-91 0.44321545499315107 1105 92-93 0.5334107747217445 1105 94-95 0.977764619652838 1105 96-97 0.7556696686003974 1105 98-99 0.7413461093394105 1105 100-101 1.4336704916036958 1106 1 -0.08061015247405123 1106 2 -0.11053345477893473 1106 3 -0.29917161315400875 1106 4 -0.14268264100785188 1106 5 0.04755259909923382 1106 6 0.0158701668943948 1106 7 0.11461691717125433 1106 8 0.058182653922784766 1106 9 -0.12043725712734954 1106 10-11 -0.3712575700103784 1106 12-13 -0.6934744504552626 1106 14-15 -0.6237257756878094 1106 16-17 -0.45200995315384773 1106 18-19 -0.030654531353597747 1106 20-21 0.0642040331482221 1106 22-23 -0.022391489080128224 1106 24-25 0.08412729822375553 1106 26-27 -0.1409598065579445 1106 28-29 0.10011602835642464 1106 30-31 -0.06030564598519561 1106 32-33 -0.14881089831772698 1106 34-35 -0.3164375795096106 1106 36-37 -0.21143431427278614 1106 38-39 -0.13252310148410373 1106 40-41 -0.13031110242500432 1106 42-43 -0.08013218673355738 1106 44-45 -0.28513745720262307 1106 46-47 0.3967769924791398 1106 48-49 0.5105672304426818 1106 50-51 0.17586877926346034 1106 52-53 0.04488236016599245 1106 54-55 0.009536824761653406 1106 56-57 0.04841667550491735 1106 58-59 -0.03140861019090124 1106 60-61 -0.0328052946319346 1106 62-63 0.045638369892131436 1106 64-65 0.2706943162417943 1106 66-67 0.40754542787131953 1106 68-69 -0.24693930497621608 1106 70-71 0.1789859190368972 1106 72-73 0.17460009673883548 1106 74-75 0.04096746419239494 1106 76-77 0.4589199841718461 1106 78-79 0.10839104181945913 1106 80-81 0.2610208154792666 1106 82-83 0.467248633395112 1106 84-85 0.3129760678035787 1106 86-87 0.47354026859611054 1106 88-89 0.5844564024831556 1106 90-91 0.5026363044140005 1106 92-93 0.42139876270973176 1106 94-95 0.3304887723769916 1106 96-97 0.6160857990165276 1106 98-99 0.7794070274003317 1106 100-101 0.5252106031438082 1107 1 0.06437840587835097 1107 2 0.14889700542956064 1107 3 8.690682596679267E-4 1107 4 0.1125067818245924 1107 5 0.1826352332207719 1107 6 0.08936622588244347 1107 7 0.08463471528973798 1107 8 0.0720499308106568 1107 9 -0.1139434864688198 1107 10-11 0.03705415132193224 1107 12-13 0.12986396805478506 1107 14-15 0.08744890013217343 1107 16-17 0.22243703387895408 1107 18-19 0.0027169408050653487 1107 20-21 -0.22591292591611278 1107 22-23 -0.05204569706386053 1107 24-25 0.1491616231665418 1107 26-27 0.21313630328187116 1107 28-29 0.095628359909945 1107 30-31 -0.07477551631318846 1107 32-33 0.05345963308323576 1107 34-35 0.1150269513828377 1107 36-37 0.03392037680273319 1107 38-39 0.18127044034147133 1107 40-41 -0.01128745635329409 1107 42-43 0.003007909884800597 1107 44-45 0.37245216903689027 1107 46-47 0.03717363626250858 1107 48-49 0.2524538310020503 1107 50-51 0.010918359736372452 1107 52-53 -0.07695338862628631 1107 54-55 0.24657470932814363 1107 56-57 -0.17018490089986216 1107 58-59 0.20263919047017254 1107 60-61 0.440085628327644 1107 62-63 0.4325084436271922 1107 64-65 0.2917774588810005 1107 66-67 0.29055076730686835 1107 68-69 0.18775939830371868 1107 70-71 0.2923395930770738 1107 72-73 0.0875647547606988 1107 74-75 0.37033690228036775 1107 76-77 0.232675387172101 1107 78-79 0.5123015427093627 1107 80-81 0.6318471566946258 1107 82-83 0.5339356407686218 1107 84-85 0.5960373441829319 1107 86-87 0.6385313695368708 1107 88-89 0.43196212330695616 1107 90-91 0.5889749771828754 1107 92-93 0.7844930927377014 1107 94-95 0.7692505318481331 1107 96-97 0.9534643904225781 1107 98-99 1.10550415427549 1107 100-101 1.0388541322564464 1108 1 -0.040218954430041975 1108 2 -0.008555731334219274 1108 3 -0.05075542190163418 1108 4 0.1663692470660365 1108 5 -0.0714476725660731 1108 6 0.05890738504164261 1108 7 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13 1661.0 14 1954.0 15 2561.0 16 3430.0 17 4448.0 18 4048.0 19 2430.0 20 2267.0 21 2470.0 22 2585.0 23 2920.0 24 3188.0 25 3549.0 26 4086.0 27 4764.0 28 5825.0 29 7242.0 30 8680.0 31 10988.0 32 14282.0 33 18297.0 34 25544.0 35 37374.0 36 56652.0 37 85912.0 38 77623.0 39 10894.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.485787862591714 20.420290559253683 15.51042223409941 21.583499344055195 2 14.693939011239884 21.059240117741922 41.33207690140571 22.91474396961248 3 18.23445267741567 26.299850155066434 30.738854524126978 24.726842643390913 4 11.885211064075756 17.625386463046482 39.037807197138044 31.451595275739713 5 12.579144859075397 38.40678089594288 35.06053503989119 13.953539205090529 6 29.643564548702813 38.439636088239865 18.16378942053752 13.753009942519807 7 27.212953209419283 34.06091173429513 21.334883472570787 17.3912515837148 8 25.855339805825245 35.83228155339806 20.084466019417476 18.227912621359224 9 26.209574661731693 17.11158303501001 21.030762696438323 35.64807960681998 10-11 23.8947709554437 27.900213502225448 27.955804189692905 20.249211352637946 12-13 25.20131902648369 25.433374477056002 28.49912797154165 20.866178524918652 14-15 22.372114529265204 26.581221579152402 27.152217417739582 23.89444647384281 16-17 20.855906368022993 30.181846425693454 27.68145807240847 21.280789133875086 18-19 20.884919238377243 28.18259043966846 30.79499532784001 20.13749499411429 20-21 21.690738363189357 27.344290384508035 30.826535738642292 20.138435513660312 22-23 21.434084256818746 27.18711925946421 30.807764558547273 20.571031925169773 24-25 21.135796357035293 27.452679196562592 30.856476509782084 20.555047936620035 26-27 20.950347036839297 27.624132407901765 30.74916274328981 20.676357811969133 28-29 20.728429325026365 27.674271006386768 30.988766417449494 20.608533251137374 30-31 20.92132218726941 27.639908345955185 30.760175152433106 20.678594314342305 32-33 20.73051004396701 27.69884967361506 30.723265135729775 20.847375146688155 34-35 21.14839852900125 27.709756896823762 30.527350624446253 20.614493949728736 36-37 21.226017282239773 27.53472647779411 30.386259636468655 20.852996603497466 38-39 20.905511094391827 27.957139303331658 30.482789585772966 20.654560016503552 40-41 21.055243697927665 27.699874043495036 30.621749460619785 20.623132797957513 42-43 20.905243026650776 27.639412377163964 30.328274677028293 21.12706991915696 44-45 21.01457101839368 27.966477070699842 30.069912248659946 20.949039662246534 46-47 21.146058225233524 28.280342278645694 29.508244468796992 21.065355027323797 48-49 21.11463464658858 28.42716415265849 29.242593597198002 21.21560760355493 50-51 21.33049345857909 28.48430787155028 29.111993009539066 21.073205660331563 52-53 21.414526148669808 28.584866956519626 28.981776162628385 21.018830732182174 54-55 21.31119687371053 28.67132697395568 28.87764266122964 21.139833491104156 56-57 21.207028562484 29.00210284144478 28.738671290571915 21.052197305499302 58-59 21.242214318736963 28.427671582278556 29.11605139611386 21.214062702870613 60-61 21.155234569429226 28.609061467468 29.247555291842154 20.988148671260618 62-63 21.108255304276916 28.57246872770165 29.46525219282278 20.85402377519865 64-65 21.17538852810701 28.322995233796366 29.25827775998136 21.243338478115263 66-67 21.04968112059175 28.576958171952207 28.99569973887805 21.377660968578002 68-69 20.896331897121794 28.58307680920793 29.19096921831831 21.329622075351963 70-71 21.039794390391293 28.510859303519293 29.135672733185615 21.3136735729038 72-73 21.01850773218394 28.429245420391997 29.091553329256097 21.46069351816796 74-75 20.979037102205503 28.872008008979765 28.76534296028881 21.383611928525923 76-77 20.984720976711955 28.62690229361305 29.04470609935309 21.343670630321903 78-79 21.088894335895315 28.656161494572274 28.788183986933657 21.466760182598758 80-81 20.913677987122202 28.929778873714586 28.683557145526873 21.47298599363634 82-83 21.127073324928194 28.852358757867684 28.639884285747225 21.3806836314569 84-85 21.143486005212555 28.679364786620138 28.535704058900897 21.64144514926641 86-87 20.959313153913072 28.65951687327782 28.698829007987403 21.682340964821705 88-89 21.140655807322474 28.29726696393363 28.804804804804807 21.757272423939092 90-91 21.026313681998314 28.339202120043584 28.99164456265607 21.64283963530203 92-93 21.080813485780013 28.31924014477824 28.923129937621106 21.676816431820637 94-95 20.915855849953104 28.364328189970745 28.746774023288914 21.973041936787236 96-97 21.07094627796847 28.35145422094743 28.63245841053062 21.94514109055348 98-99 20.887128088142504 28.29199631121681 28.607605688491965 22.213269912148718 100-101 20.953325026177247 28.279921231659245 28.810624747479036 21.95612899468447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 553.0 1 311.0 2 91.0 3 114.5 4 106.5 5 114.5 6 192.0 7 404.0 8 651.0 9 783.0 10 740.5 11 634.5 12 656.5 13 714.5 14 724.5 15 719.5 16 749.0 17 821.5 18 877.0 19 900.0 20 995.5 21 1156.5 22 1324.0 23 1557.5 24 1818.0 25 2232.0 26 2891.5 27 3739.0 28 4412.0 29 5082.0 30 5994.5 31 6928.5 32 8092.5 33 9341.5 34 10586.5 35 11750.5 36 13261.0 37 14608.5 38 14979.5 39 14791.0 40 14487.5 41 14616.0 42 14720.0 43 14958.0 44 15524.5 45 15680.0 46 15260.0 47 15054.0 48 15497.5 49 14872.5 50 13526.5 51 12607.0 52 11673.5 53 11089.5 54 10525.5 55 9408.0 56 8247.5 57 7301.0 58 6572.5 59 5764.0 60 4689.5 61 3757.5 62 2878.0 63 2092.0 64 1574.5 65 1157.0 66 824.5 67 609.5 68 438.5 69 321.0 70 229.0 71 169.5 72 131.5 73 109.0 74 87.0 75 57.5 76 39.0 77 25.0 78 15.5 79 12.0 80 4.5 81 5.5 82 5.0 83 6.0 84 5.0 85 1.0 86 1.0 87 1.5 88 2.0 89 2.0 90 2.0 91 1.5 92 1.5 93 1.0 94 0.0 95 1.0 96 1.5 97 2.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27957187225939617 2 0.24856216719076296 3 0.2458972706614273 4 0.2488044305116117 5 0.24928895715330907 6 0.19526423660404968 7 0.1860582304117992 8 0.18751181033689138 9 0.18145522731567396 10-11 0.20216874124823753 12-13 0.19465857830192793 14-15 0.18763294199731573 16-17 0.18848086362028615 18-19 0.1838778605241609 20-21 0.17951712074888437 22-23 0.18121296399482525 24-25 0.1743084593506374 26-27 0.17297601108596958 28-29 0.181818622296947 30-31 0.19138802347047054 32-33 0.18424125550543397 34-35 0.19599102656659576 36-37 0.17745788252167047 38-39 0.17963825240930872 40-41 0.1761254342570026 42-43 0.17951712074888437 44-45 0.1835144655428879 46-47 0.18666388871392095 48-49 0.18957104856410528 50-51 0.18993444354537833 52-53 0.20374345283375406 54-55 0.18993444354537833 56-57 0.17261261610469653 58-59 0.1743084593506374 60-61 0.17212808946299912 62-63 0.18121296399482525 64-65 0.17103790451918 66-67 0.17103790451918 68-69 0.16825187632941999 70-71 0.17709448754039742 72-73 0.17685222421954871 74-75 0.17842693580506525 76-77 0.17891146244676262 78-79 0.17515638097360783 80-81 0.18097070067397658 82-83 0.17551977595488089 84-85 0.16764621802729823 86-87 0.1665560330834791 88-89 0.1663137697626304 90-91 0.17128016784002867 92-93 0.16849413965026866 94-95 0.1670405597251765 96-97 0.1648601898375382 98-99 0.17297601108596958 100-101 0.16461792651668952 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 412774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.42377212537576 #Duplication Level Percentage of deduplicated Percentage of total 1 84.23034016174465 55.948969209466114 2 9.300501672332185 12.355488074693385 3 2.591335487899428 5.1637883384589305 4 1.1196936829619124 2.974971121891392 5 0.6476503678980366 2.150969022708748 6 0.4115723238685186 1.64029117522523 7 0.2860606953726308 1.330086131042071 8 0.21512851009809433 1.143171770594193 9 0.18598076555229368 1.111818959167364 >10 0.9467286888337987 11.300318666119075 >50 0.05186003016628356 2.348972256936515 >100 0.011686767353005018 1.1020738363432605 >500 3.652114797814068E-4 0.12857123778602864 >1k 0.0010956344393442204 1.3005101995677348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2224 0.5387936255675018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1838 0.44527998371990485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1299 0.31470005378245725 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 530 0.12839956004980932 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 438 0.10611133453172923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.4226332084869686E-4 0.0 26-27 0.0 0.0 0.0 6.056583021217421E-4 0.0 28-29 0.0 0.0 0.0 0.0021803698876382716 0.0 30-31 0.0 0.0 0.0 0.00460300309612524 0.0 32-33 0.0 0.0 0.0 0.007025636304612209 0.0 34-35 0.0 0.0 0.0 0.00932713785267483 0.0 36-37 0.0 0.0 0.0 0.011022981098615707 0.0 38-39 0.0 0.0 0.0 0.014051272609224418 0.0 40-41 0.0 0.0 0.0 0.017442959101106173 0.0 42-43 0.0 0.0 0.0 0.024226332084869687 0.0 44-45 0.0 0.0 0.0 0.037187419750274966 0.0 46-47 0.0 0.0 0.0 0.049785112434407204 0.0 48-49 0.0 0.0 0.0 0.08091594916346476 0.0 50-51 0.0 0.0 0.0 0.11640752566779884 0.0 52-53 0.0 0.0 0.0 0.1500821272657677 0.0 54-55 0.0 0.0 0.0 0.19914044973762884 0.0 56-57 0.0 0.0 0.0 0.25425535523070736 0.0 58-59 0.0 0.0 0.0 0.33420225111077734 0.0 60-61 0.0 0.0 0.0 0.43680076749020047 0.0 62-63 0.0 0.0 0.0 0.5840968665662082 0.0 64-65 0.0 0.0 0.0 0.7104371883888037 0.0 66-67 0.0 0.0 0.0 0.8845033844185923 0.0 68-69 0.0 0.0 0.0 1.1119886426955188 0.0 70-71 0.0 0.0 0.0 1.270307722870142 0.0 72-73 0.0 0.0 0.0 1.4929477147300945 0.0 74-75 0.0 0.0 0.0 1.7945655491867218 0.0 76-77 0.0 0.0 0.0 2.0207183591989804 0.0 78-79 0.0 0.0 0.0 2.2363327147543206 0.0 80-81 0.0 0.0 0.0 2.576349285565467 0.0 82-83 0.0 0.0 0.0 2.9776584765513334 0.0 84-85 0.0 0.0 0.0 3.3865989621439336 0.0 86-87 0.0 0.0 0.0 3.9295110641658635 0.0 88-89 0.0 0.0 0.0 4.589072955176441 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 44.20064 1 GTATCAA 2370 0.0 34.902447 1 TATCAAC 2930 0.0 27.738178 2 ATCAACG 2955 0.0 27.181831 3 GGACCGA 70 0.008386165 27.14237 6 TCAACGC 2960 0.0 27.135916 4 CAACGCA 2975 0.0 26.999096 5 GTACCTG 300 0.0 26.939074 1 AACGCAG 3060 0.0 26.23319 6 GAGTACT 1870 0.0 25.019606 12-13 TACCTGG 330 0.0 24.484118 2 GTACATG 2005 0.0 24.42181 1 GTACTTT 1965 0.0 23.810009 14-15 GTCTTAG 120 1.6986087E-4 23.76977 1 CATGGGT 280 1.8189894E-12 23.763996 4 GGGTATG 140 1.7761346E-5 23.749573 7 ACGCAGA 3395 0.0 23.224916 7 TACATGG 2110 0.0 23.200869 2 ATGGGTA 165 2.411216E-6 23.043875 5 CGCAGAG 3425 0.0 23.021486 8 >>END_MODULE