FastQCFastQC Report
Thu 26 May 2016
SRR937274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412774
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.6989296806484905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.6272197376772761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.4084559589509029No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.14341988594242855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.048.3636131
GTATCAA23750.036.4228061
TCGTATA600.003945308331.671152
CATATAG1004.940752E-528.5040343
ATCAACG30900.027.9813083
TCAACGC31550.027.4048294
CAACGCA31750.027.2322035
TATCAAC31800.027.1893832
TCGTAGC700.00837965827.1467027
TAGTACA700.00837965827.1467024
AACGCAG32200.026.8451216
TATTCGT908.930409E-426.3926244
GTATAGA1751.4723992E-724.4438781
TAGAGTG1000.001650495723.7533635
ACGCAGA36900.023.4315017
CGCAGAG36800.023.3660778
TTCGTAG1050.00219513922.616776
GAGTACT24950.022.37290812-13
GCAGAGT38450.022.2398179
AGTACTT26000.021.9261812-13
Produced by FastQC (version 0.11.5)