##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 412774 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22306395267144 33.0 31.0 34.0 31.0 34.0 2 32.44402021445149 34.0 31.0 34.0 31.0 34.0 3 32.51383081298725 34.0 31.0 34.0 31.0 34.0 4 36.13089244962134 37.0 35.0 37.0 35.0 37.0 5 36.00341591282397 37.0 35.0 37.0 35.0 37.0 6 36.0962269910411 37.0 35.0 37.0 35.0 37.0 7 36.06358443118995 37.0 35.0 37.0 35.0 37.0 8 36.047648349944524 37.0 35.0 37.0 35.0 37.0 9 37.74929137978652 39.0 37.0 39.0 35.0 39.0 10-11 37.6778660962173 39.0 37.0 39.0 35.0 39.0 12-13 37.60117158541962 39.0 37.0 39.0 35.0 39.0 14-15 38.81888273001691 40.0 38.0 41.0 35.0 41.0 16-17 38.79013939831482 40.0 38.0 41.0 35.0 41.0 18-19 38.841590071080056 40.0 38.0 41.0 35.0 41.0 20-21 38.77536133574304 40.0 38.0 41.0 35.0 41.0 22-23 38.65326667861832 40.0 38.0 41.0 34.0 41.0 24-25 38.511336712099116 40.0 38.0 41.0 34.0 41.0 26-27 38.29380968762567 40.0 38.0 41.0 34.0 41.0 28-29 38.28360313391832 40.0 38.0 41.0 34.0 41.0 30-31 38.36195109188078 40.0 38.0 41.0 34.0 41.0 32-33 38.26766826398949 40.0 38.0 41.0 34.0 41.0 34-35 38.148958025457034 40.0 37.5 41.0 34.0 41.0 36-37 37.99023073158678 40.0 37.0 41.0 33.5 41.0 38-39 37.83590899620616 40.0 37.0 41.0 33.0 41.0 40-41 37.66989805559459 40.0 37.0 41.0 33.0 41.0 42-43 37.43422550838958 40.0 37.0 41.0 33.0 41.0 44-45 37.09143502255471 40.0 37.0 41.0 32.0 41.0 46-47 36.67814833298608 40.0 36.0 41.0 31.0 41.0 48-49 36.29954284911356 40.0 36.0 41.0 30.0 41.0 50-51 36.0829812439737 39.0 36.0 41.0 29.5 41.0 52-53 35.85686719609278 39.0 35.5 41.0 29.0 41.0 54-55 35.64882356931396 39.0 35.0 40.5 28.0 41.0 56-57 35.42202755018485 39.0 35.0 40.0 28.0 41.0 58-59 35.18592256295213 38.5 35.0 40.0 27.0 41.0 60-61 34.876765493950685 38.0 34.5 40.0 26.5 41.0 62-63 34.538156473033666 38.0 34.0 40.0 26.0 41.0 64-65 34.202470359082696 37.5 34.0 40.0 26.0 41.0 66-67 33.82739828574474 37.0 34.0 39.5 25.0 41.0 68-69 33.39456579145005 36.0 33.0 39.0 23.5 40.5 70-71 33.01855131379399 36.0 33.0 39.0 22.5 40.0 72-73 32.58725476895347 35.0 32.5 38.0 22.0 40.0 74-75 32.150921569672505 35.0 32.0 37.5 20.5 39.5 76-77 30.805690280880093 34.0 30.5 36.0 19.5 38.5 78-79 31.412918449320934 35.0 31.5 36.0 19.5 39.0 80-81 31.23144020698978 35.0 31.5 36.0 18.5 38.5 82-83 30.889088217765654 35.0 31.0 36.0 17.5 37.0 84-85 30.544523153105573 34.0 31.0 35.0 13.5 37.0 86-87 30.221667304626745 34.0 31.0 35.0 8.5 36.0 88-89 29.855770954565937 34.0 30.5 35.0 4.5 36.0 90-91 29.429222770814054 34.0 30.0 35.0 2.0 36.0 92-93 29.034035573946035 34.0 29.5 35.0 2.0 35.0 94-95 28.642143158241556 34.0 29.0 35.0 2.0 35.0 96-97 28.255248634846186 34.0 29.0 35.0 2.0 35.0 98-99 27.657881794880492 33.5 28.5 35.0 2.0 35.0 100-101 26.07078086313576 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.052386038573231986 1101 2 0.05173149961768786 1101 3 0.04216807573590842 1101 4 0.11459281625830187 1101 5 -0.032210617574378375 1101 6 -0.0461531012483718 1101 7 0.06748090809713148 1101 8 -0.08646783418203796 1101 9 0.048898556740304855 1101 10-11 0.09739458861434258 1101 12-13 0.10279695893287055 1101 14-15 0.23661427421509273 1101 16-17 0.18950749799356714 1101 18-19 0.2579039863530639 1101 20-21 0.2556934908149415 1101 22-23 0.1717953613628893 1101 24-25 0.2746822146117722 1101 26-27 0.14499230511506767 1101 28-29 -0.021241695325350918 1101 30-31 0.05341150267543071 1101 32-33 0.08106587279944222 1101 34-35 0.203855203817632 1101 36-37 0.19552140472630697 1101 38-39 0.17394140704265482 1101 40-41 0.25320146896637397 1101 42-43 0.49421243551542915 1101 44-45 0.344124120716792 1101 46-47 0.29747600494607696 1101 48-49 0.48090283063803696 1101 50-51 0.3680830016049583 1101 52-53 0.4630059669944089 1101 54-55 0.5824387109938769 1101 56-57 0.5899336256077561 1101 58-59 0.7064568181691513 1101 60-61 0.5953696050568098 1101 62-63 0.5746677104780673 1101 64-65 0.5930519293361556 1101 66-67 0.41086687284118995 1101 68-69 0.42401499269487175 1101 70-71 0.27232639627438004 1101 72-73 0.2628465781125726 1101 74-75 0.43597803651596223 1101 76-77 0.46694329475453245 1101 78-79 0.6313020729435337 1101 80-81 0.6092009301228387 1101 82-83 0.3830512362354419 1101 84-85 0.5480925001587522 1101 86-87 0.3186824122858667 1101 88-89 0.299374731654364 1101 90-91 0.501188728391103 1101 92-93 0.623202761249189 1101 94-95 0.3975689790286374 1101 96-97 0.33972166839037854 1101 98-99 -0.061648705214704336 1101 100-101 0.4194122344239659 1102 1 0.1050475279364349 1102 2 0.06180117481899572 1102 3 0.006085855710686872 1102 4 0.10049339230571519 1102 5 -0.060465825601937695 1102 6 0.13994567456280294 1102 7 0.08080538036677609 1102 8 0.2424357500869121 1102 9 0.3680072214046177 1102 10-11 0.14471713054979318 1102 12-13 0.20817747195088288 1102 14-15 0.2759418979549153 1102 16-17 0.27475570562425133 1102 18-19 0.27393022199003525 1102 20-21 0.17624098243724262 1102 22-23 0.2195899193234041 1102 24-25 0.2544195764414141 1102 26-27 0.0030258534779150636 1102 28-29 -0.06447695421792332 1102 30-31 -0.0785862523130163 1102 32-33 -0.07928806876920191 1102 34-35 -0.06953953073793429 1102 36-37 -0.13019313595044224 1102 38-39 -0.14167762671001327 1102 40-41 -0.21271592118422689 1102 42-43 0.019105997075946846 1102 44-45 0.19801637425280205 1102 46-47 -0.018535201324922923 1102 48-49 -0.08534966740697314 1102 50-51 -0.028039421974241918 1102 52-53 -0.07054239586144462 1102 54-55 -0.15548554569829776 1102 56-57 -0.1601972942598877 1102 58-59 -0.14203660530906603 1102 60-61 0.09766158336756092 1102 62-63 -0.009874821453458082 1102 64-65 0.13224687195450713 1102 66-67 0.08414959165349956 1102 68-69 -0.07505271694716953 1102 70-71 -0.528401823473736 1102 72-73 -0.32437893047882227 1102 74-75 -0.4169161731859603 1102 76-77 -0.5519796163397253 1102 78-79 -0.1489110622526546 1102 80-81 -0.1502211907555342 1102 82-83 0.03140399424151852 1102 84-85 -0.002803038702726468 1102 86-87 -0.015234874537839005 1102 88-89 0.1079464017691727 1102 90-91 -0.14981882621363596 1102 92-93 0.11437300874057854 1102 94-95 0.0395495206963794 1102 96-97 0.15694226895444885 1102 98-99 -0.24087154609364347 1102 100-101 0.08639083871250364 1103 1 0.12915264087912703 1103 2 0.09156291809208028 1103 3 0.06242155298193808 1103 4 -0.018336539814718833 1103 5 0.06956342066902721 1103 6 0.0014545433995181156 1103 7 0.09153805642770152 1103 8 0.10821761305809474 1103 9 -0.021904171618601254 1103 10-11 0.2053366740041156 1103 12-13 0.15492918250504317 1103 14-15 0.21151735496181345 1103 16-17 0.12199548706399099 1103 18-19 0.15771292056896158 1103 20-21 0.14629068481981022 1103 22-23 0.18801452742879832 1103 24-25 -0.0425928545802563 1103 26-27 -0.12505951958838324 1103 28-29 0.06217187891555653 1103 30-31 0.07998930778833824 1103 32-33 0.24863913568882623 1103 34-35 0.18005894346402584 1103 36-37 0.21004164812092796 1103 38-39 0.1860745884644146 1103 40-41 0.2105519562093221 1103 42-43 0.454159199244188 1103 44-45 0.2691610483386526 1103 46-47 0.15696955458537332 1103 48-49 0.16475071404253328 1103 50-51 0.1631750307840747 1103 52-53 0.10271462948351484 1103 54-55 0.06371905339990036 1103 56-57 -0.13330456133423496 1103 58-59 -0.11903433180488321 1103 60-61 -0.04274222136067607 1103 62-63 0.010101086998659525 1103 64-65 0.08274118276803222 1103 66-67 -0.1865439085119931 1103 68-69 0.12347330355235187 1103 70-71 0.1204001103613237 1103 72-73 -0.07220038172328458 1103 74-75 -0.19290818607819205 1103 76-77 -0.20616280977206358 1103 78-79 0.16105464527378288 1103 80-81 0.1052346766758383 1103 82-83 0.004249455643492439 1103 84-85 -0.03503497953735746 1103 86-87 0.12748177304730746 1103 88-89 0.15511754400155198 1103 90-91 0.39753680084486476 1103 92-93 0.32053615843916106 1103 94-95 0.08936449326782636 1103 96-97 0.2518515729583122 1103 98-99 0.2434730173013122 1103 100-101 0.4492290835021642 1104 1 -0.031184810450007205 1104 2 -0.05264104287508786 1104 3 -0.1818941707117574 1104 4 -0.020481663114239268 1104 5 -0.18014215064280137 1104 6 -0.22455252288899885 1104 7 -0.047852896800200995 1104 8 0.05951069578654966 1104 9 0.07293791846456088 1104 10-11 -0.020820611002861256 1104 12-13 -0.12305055599421166 1104 14-15 0.019087176622157642 1104 16-17 -0.09265203973502167 1104 18-19 -0.14591516191914167 1104 20-21 0.023231510662235166 1104 22-23 -0.2325347923808394 1104 24-25 -0.22490430004484807 1104 26-27 -0.48878710695558425 1104 28-29 -0.354327544244569 1104 30-31 -0.25589373792622894 1104 32-33 -0.23869742634549596 1104 34-35 -0.43962030952832976 1104 36-37 -0.11666002952171795 1104 38-39 -0.17810365505815895 1104 40-41 -0.16452014877553012 1104 42-43 -0.3773314511427799 1104 44-45 -0.347916416450083 1104 46-47 -0.8352280851781231 1104 48-49 -1.0040530642586702 1104 50-51 -0.8788561666393662 1104 52-53 -0.8922650609457534 1104 54-55 -0.6360297809028523 1104 56-57 -0.7332480790960929 1104 58-59 -0.8110408933585092 1104 60-61 -0.9871883349019939 1104 62-63 -0.9806184005752527 1104 64-65 -0.9271672439012022 1104 66-67 -0.8594186140833102 1104 68-69 -0.8775211443638185 1104 70-71 -0.8904607029829066 1104 72-73 -0.6444585896740591 1104 74-75 -0.8422195834728008 1104 76-77 -0.8783975836027587 1104 78-79 -0.9992617754347712 1104 80-81 -0.7162003326978059 1104 82-83 -0.5866629127649112 1104 84-85 -0.6972291342265855 1104 86-87 -0.7225699021918395 1104 88-89 -0.6024745100291184 1104 90-91 -0.5566615118984934 1104 92-93 -0.9154334132572295 1104 94-95 -1.1801038526438212 1104 96-97 -1.025229857604586 1104 98-99 -0.7506414133096584 1104 100-101 -0.937130455987333 1105 1 0.07200187724372142 1105 2 0.032389410113111694 1105 3 -0.015490342336100582 1105 4 0.08345955174246455 1105 5 -0.17868067911091146 1105 6 -0.05037910399727252 1105 7 -0.013334978479463189 1105 8 0.04995335172215931 1105 9 0.10477760523382784 1105 10-11 0.1072994607791884 1105 12-13 -0.046989459686642476 1105 14-15 0.03536728973325154 1105 16-17 -0.0889167904011785 1105 18-19 -0.011670504787161917 1105 20-21 0.09748785956647055 1105 22-23 -0.06941757556644035 1105 24-25 -0.0746174437720768 1105 26-27 -0.18110996301083304 1105 28-29 -0.07412946387042751 1105 30-31 -0.03628554824760499 1105 32-33 0.043696260097490836 1105 34-35 0.10471665822413456 1105 36-37 0.19089475866686456 1105 38-39 0.2616837178278004 1105 40-41 0.1671387233762971 1105 42-43 0.07304649219291548 1105 44-45 0.24502977420737437 1105 46-47 0.1161456980618567 1105 48-49 0.057621034387260295 1105 50-51 0.05395013060354614 1105 52-53 0.013150540772663533 1105 54-55 0.2108555853487175 1105 56-57 -0.005700360351191591 1105 58-59 0.03539923389148214 1105 60-61 0.29667362061488944 1105 62-63 0.10345699776071626 1105 64-65 0.31314625873623925 1105 66-67 0.33886228754266057 1105 68-69 0.4281269332913027 1105 70-71 0.17804832415288274 1105 72-73 0.25363605930087374 1105 74-75 0.3381939789830142 1105 76-77 0.28296246860235996 1105 78-79 0.3569940024759397 1105 80-81 0.35897708314861987 1105 82-83 0.07563520023742498 1105 84-85 -0.19704560059234666 1105 86-87 0.37529431010899117 1105 88-89 0.26255689461496345 1105 90-91 0.11386502570551116 1105 92-93 -0.17486160402344808 1105 94-95 0.08111209167071465 1105 96-97 0.16841172160037488 1105 98-99 0.12370942142850083 1105 100-101 -0.021035915773076397 1106 1 -0.2113100060681603 1106 2 -0.11800098027728012 1106 3 -0.1058378326835907 1106 4 -0.25286820458529036 1106 5 -0.04194966237989206 1106 6 -0.18232247594064432 1106 7 -0.1876807528252371 1106 8 -0.04591966415085125 1106 9 -0.16136568090946213 1106 10-11 -0.16925566577594253 1106 12-13 -0.3654879581851347 1106 14-15 -0.10401923965327597 1106 16-17 -0.029127000611389064 1106 18-19 -0.2489120320286915 1106 20-21 -0.3216198195412048 1106 22-23 -0.16687360302246645 1106 24-25 -0.18743168658632925 1106 26-27 -0.28854454044541455 1106 28-29 -0.19051299025395707 1106 30-31 -0.09547902744108683 1106 32-33 0.002370648771872652 1106 34-35 0.014382037889511423 1106 36-37 0.15040569817780636 1106 38-39 0.14236582850991653 1106 40-41 0.14838282462039842 1106 42-43 0.02564194478836157 1106 44-45 0.47804993722754574 1106 46-47 0.5307617426778961 1106 48-49 0.2370032737694956 1106 50-51 0.585048371701788 1106 52-53 0.4220737496958762 1106 54-55 0.4669044913976208 1106 56-57 0.8038019991511689 1106 58-59 0.5687282772205222 1106 60-61 0.7764462503875222 1106 62-63 0.807249361553076 1106 64-65 0.5965182021081858 1106 66-67 0.8763912450716163 1106 68-69 0.6937425489069184 1106 70-71 0.7553413314458837 1106 72-73 0.729058624723443 1106 74-75 0.7553625761516116 1106 76-77 0.5165789422188354 1106 78-79 0.4621505876325216 1106 80-81 0.811628305799843 1106 82-83 0.5452619698641961 1106 84-85 0.4097583462115999 1106 86-87 0.6546679694826523 1106 88-89 0.5613556934137627 1106 90-91 0.6833502251907078 1106 92-93 0.6989093097474637 1106 94-95 0.48036563092425055 1106 96-97 0.698643381832035 1106 98-99 0.7166394943585708 1106 100-101 0.7210189962818347 1107 1 -0.09035907802341114 1107 2 0.04410936805732746 1107 3 0.006747979673392024 1107 4 0.013122133578960415 1107 5 0.12599846881767007 1107 6 2.557086512737783E-4 1107 7 -0.02198026973345435 1107 8 -0.06495348057597283 1107 9 -0.020674096876916792 1107 10-11 -0.17187453178153334 1107 12-13 0.07973451829592193 1107 14-15 0.02422383179345644 1107 16-17 0.005538649019932507 1107 18-19 -0.03179278463484536 1107 20-21 -0.05524046535865068 1107 22-23 -0.13412252242241607 1107 24-25 -0.01988528434884529 1107 26-27 -0.1078326157060232 1107 28-29 -0.1882322884995702 1107 30-31 -0.17343732899206543 1107 32-33 -0.12445620096114851 1107 34-35 -0.32664008262917577 1107 36-37 -0.18404891662005696 1107 38-39 0.14048177053890498 1107 40-41 -0.18801178509229288 1107 42-43 -0.5177091866634527 1107 44-45 -0.5231730477711878 1107 46-47 -0.39212409510496116 1107 48-49 -0.05997613126482548 1107 50-51 -0.04680263262061146 1107 52-53 0.16893365307752362 1107 54-55 -0.06005965810657443 1107 56-57 -0.07862871531616378 1107 58-59 0.3597478551508573 1107 60-61 0.417356497018595 1107 62-63 0.41817740630263245 1107 64-65 0.3430933355940766 1107 66-67 0.21973220617001488 1107 68-69 0.2831272425250191 1107 70-71 -0.083028869418591 1107 72-73 0.3971160872202617 1107 74-75 0.39970277447351066 1107 76-77 0.43258962108992804 1107 78-79 0.34120982994119586 1107 80-81 0.3726275430233379 1107 82-83 0.6520888195972248 1107 84-85 0.4403889081236265 1107 86-87 0.48877424967588823 1107 88-89 0.5704454467826885 1107 90-91 0.5700245196224394 1107 92-93 0.6683524828977312 1107 94-95 0.6459186438914521 1107 96-97 0.8332200408709376 1107 98-99 0.33387570081674767 1107 100-101 0.7973246154021005 1108 1 0.024739980348577717 1108 2 0.03876620255347518 1108 3 0.06873429445577273 1108 4 0.03427659302405317 1108 5 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76-77 -0.32003734661407535 2316 78-79 -0.14027314592471285 2316 80-81 -0.14801065305825745 2316 82-83 -0.4383232526379004 2316 84-85 -0.5679718525009676 2316 86-87 -0.7263171970440752 2316 88-89 -0.7895399125196292 2316 90-91 -0.7261124112492574 2316 92-93 -0.8270016894129029 2316 94-95 -0.788114637757527 2316 96-97 -0.7796689607372365 2316 98-99 -0.7537523332372835 2316 100-101 -0.7119756860579329 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 3.0 6 10.0 7 38.0 8 105.0 9 200.0 10 321.0 11 566.0 12 900.0 13 1311.0 14 1703.0 15 2505.0 16 3504.0 17 5248.0 18 4674.0 19 2371.0 20 1981.0 21 1845.0 22 1882.0 23 2001.0 24 2278.0 25 2648.0 26 3253.0 27 3955.0 28 5006.0 29 6361.0 30 8066.0 31 10169.0 32 12880.0 33 17279.0 34 23923.0 35 35579.0 36 56325.0 37 94563.0 38 89580.0 39 9739.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56569708398068 20.808419154448924 16.37973450190628 21.246149259664115 2 15.331634259909782 21.38143390814344 40.52290115171983 22.764030680226952 3 18.462645418558342 26.009147862995246 31.49665434353908 24.031552374907335 4 12.237234509040078 18.255796769653053 38.806075009751126 30.700893711555743 5 12.616266532925325 38.26359158495604 35.04192742593952 14.078214456179117 6 29.705359349183812 38.5392490806107 17.780674170369256 13.974717399836232 7 26.77639580012307 34.3931546076061 21.499173882075905 17.331275710194923 8 25.94349450304525 35.70670633324774 19.907988390741664 18.44181077296535 9 26.222097322021256 16.756627113141818 20.96667910285047 36.05459646198646 10-11 23.922170485544147 27.831452562419145 27.81207149675125 20.434305455285457 12-13 25.33839850952022 25.30205716251608 28.597490508851543 20.762053819112154 14-15 22.46859056045197 26.36503268132198 27.4083396725569 23.758037085669155 16-17 20.54610997785713 29.915280516699212 28.101091638523744 21.43751786691991 18-19 20.6126112594301 27.4446791706842 31.82455774830779 20.11815182157791 20-21 21.201553392413285 26.547335830260625 32.22550354431238 20.025607233013705 22-23 21.08811729591441 26.292251337312972 32.19023761531902 20.4293937514536 24-25 20.822779673499745 26.683649428315665 32.214070012101125 20.27950088608346 26-27 20.689078745107732 26.937133041886675 32.05259122244067 20.32119699056493 28-29 20.472594509211074 26.857477880822938 32.3749164171294 20.295011192836583 30-31 20.595944986711824 26.850230029580423 32.110385126934176 20.443439856773576 32-33 20.48808791251387 26.94065033165849 31.967493107608526 20.603768648219123 34-35 20.904901956034053 26.812493034929524 31.81401929385087 20.46858571518555 36-37 21.074365148967715 26.62740385780112 31.813050240567474 20.485180752663684 38-39 20.741641602476903 27.051155173478918 31.84761134132884 20.35959188271534 40-41 20.822895821926767 27.008605193156544 31.91334725539884 20.25515172951785 42-43 20.753912392453124 26.91973144475646 31.616661861230305 20.709694301560113 44-45 20.826650903399923 27.038040186639662 31.244579358196013 20.8907295517644 46-47 20.9613493097918 27.5655685677877 30.64873272056864 20.824349401851862 48-49 20.972614554211262 27.79959493572754 30.232160940369308 20.99562956969189 50-51 21.309966228493074 27.829151060871084 30.00600813035705 20.854874580278796 52-53 21.33870027993561 27.991864787831584 29.704184013750883 20.965250918481928 54-55 21.15079922669548 28.032894513704836 29.843328310407145 20.972977949192536 56-57 21.203006972338372 28.261469956925584 29.646368230557158 20.889154840178886 58-59 21.188713436408303 27.822004292906044 29.772587420719326 21.216694849966323 60-61 20.976854162326116 28.07541172651378 30.165175132154644 20.78255897900546 62-63 21.15952070624603 28.021992664266644 30.069723383740254 20.748763245747067 64-65 21.00435104924244 27.81425186663889 30.032899358971253 21.148497725147415 66-67 20.885763153687005 28.07565398983463 29.84223812546333 21.196344731015035 68-69 20.983031877007758 27.960700044091922 29.8341223042149 21.222145774685423 70-71 21.01016536894281 27.892139524291743 29.916128438322183 21.181566668443264 72-73 20.88309825715767 27.962880413979562 29.821888006512037 21.332133322350728 74-75 20.933489027894197 28.29405437357973 29.294723020345277 21.477733578180796 76-77 20.91422909388673 27.96360720394211 29.758172753128832 21.363990949042332 78-79 20.99562956969189 28.02768585230659 29.47617824766095 21.500506330340574 80-81 20.909383827469753 28.369034871382404 29.284547960869627 21.437033340278216 82-83 20.93545815960908 28.280329771229084 29.430119848535867 21.354092220625965 84-85 20.862021348243832 28.154026174129182 29.27255592648762 21.711396551139366 86-87 20.705761506296426 28.02187153260622 29.23088663530164 22.041480325795714 88-89 20.88285599383682 27.797899092481597 29.402651329783367 21.916593583898212 90-91 20.734047424643663 27.88554025667553 29.6424617009781 21.73795061770271 92-93 20.707570539617628 27.70284266209909 29.371954225714664 22.21763257256862 94-95 20.53242210022918 27.652904494953656 29.53274673307912 22.281926671738045 96-97 20.777108054286366 27.839689515327997 29.175771729808563 22.20743070057707 98-99 20.525384329320957 27.617564010470865 29.17402053713437 22.68303112307381 100-101 20.137865030377554 27.816874534342084 29.321275190957603 22.72398524432276 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 151.0 1 109.0 2 84.0 3 113.5 4 110.0 5 100.0 6 184.0 7 413.5 8 689.5 9 903.5 10 878.0 11 759.0 12 791.0 13 828.5 14 883.0 15 927.5 16 951.5 17 988.0 18 1037.0 19 1083.5 20 1136.0 21 1234.5 22 1405.0 23 1675.0 24 1965.5 25 2333.5 26 2942.5 27 3774.0 28 4422.5 29 5126.5 30 6042.0 31 6979.0 32 7979.5 33 9185.0 34 10441.0 35 11479.0 36 12934.0 37 14267.0 38 14884.5 39 14735.0 40 14489.5 41 14779.5 42 14661.0 43 14804.5 44 15504.5 45 15783.0 46 15394.5 47 15097.5 48 15404.5 49 14790.0 50 13612.5 51 12799.0 52 11850.0 53 11144.0 54 10543.5 55 9366.0 56 8114.0 57 7194.5 58 6472.0 59 5643.0 60 4564.0 61 3675.0 62 2828.0 63 2057.0 64 1520.5 65 1070.0 66 750.5 67 579.0 68 415.5 69 263.0 70 193.0 71 151.5 72 111.0 73 97.0 74 70.0 75 51.0 76 36.0 77 18.5 78 13.5 79 8.0 80 1.5 81 0.5 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046272294282101105 2 0.0 3 0.0 4 2.4226332084869686E-4 5 0.008963742871401784 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005087529737822634 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014535799250921812 24-25 6.056583021217421E-4 26-27 0.0013324482646678328 28-29 0.0024226332084869686 30-31 2.4226332084869686E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.2113166042434843E-4 40-41 0.0 42-43 0.011386376079888754 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.2113166042434843E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.267899625460906E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00847921622970439 92-93 0.0023015015480626205 94-95 0.0 96-97 0.0 98-99 0.0023015015480626205 100-101 0.012476561023707887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 412774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.09008809583155 #Duplication Level Percentage of deduplicated Percentage of total 1 83.18313592315543 53.31214509402561 2 9.754602060072083 12.503466107395994 3 2.84577290814566 5.471575091513584 4 1.2272314513535962 3.146134873249088 5 0.6855624394656643 2.1968878570273804 6 0.4740943345526848 1.823084860032972 7 0.31183672627188047 1.398995028079637 8 0.23710322193330613 1.2156773105208871 9 0.18003081438302276 1.0384371678395006 >10 1.0216273395713875 11.74266665335197 >50 0.057352980510940926 2.4651918506471864 >100 0.020130516332830126 1.798686933991855 >500 3.798210628835873E-4 0.14410917105469997 >1k 0.0011394631886507617 1.7429420012696821 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2885 0.6989296806484905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2589 0.6272197376772761 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1686 0.4084559589509029 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.14341988594242855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.4226332084869686E-4 0.0 26-27 0.0 0.0 0.0 6.056583021217421E-4 0.0 28-29 0.0 0.0 0.0 0.0021803698876382716 0.0 30-31 0.0 0.0 0.0 0.00460300309612524 0.0 32-33 0.0 0.0 0.0 0.007025636304612209 0.0 34-35 0.0 0.0 0.0 0.00932713785267483 0.0 36-37 0.0 0.0 0.0 0.011022981098615707 0.0 38-39 0.0 0.0 0.0 0.013809009288375721 0.0 40-41 0.0 0.0 0.0 0.016958432459408777 0.0 42-43 0.0 0.0 0.0 0.023741805443172292 0.0 44-45 0.0 0.0 0.0 0.036339498127304526 0.0 46-47 0.0 0.0 0.0 0.04857379583016372 0.0 48-49 0.0 0.0 0.0 0.07922010591752388 0.0 50-51 0.0 0.0 0.0 0.11519620906355536 0.0 52-53 0.0 0.0 0.0 0.14947646896364597 0.0 54-55 0.0 0.0 0.0 0.19792913313338534 0.0 56-57 0.0 0.0 0.0 0.2518327220222204 0.0 58-59 0.0 0.0 0.0 0.332021881223139 0.0 60-61 0.0 0.0 0.0 0.43583171420680566 0.0 62-63 0.0 0.0 0.0 0.5839757349057838 0.0 64-65 0.0 0.0 0.0 0.7106794517096522 0.0 66-67 0.0 0.0 0.0 0.8832920678143488 0.0 68-69 0.0 0.0 0.0 1.1146535392248542 0.0 70-71 0.0 0.0 0.0 1.2760009109100863 0.0 72-73 0.0 0.0 0.0 1.5011846676389502 0.0 74-75 0.0 0.0 0.0 1.8080111634938247 0.0 76-77 0.0 0.0 0.0 2.0370711333562674 0.0 78-79 0.0 0.0 0.0 2.254502463817973 0.0 80-81 0.0 0.0 0.0 2.5971839311584546 0.0 82-83 0.0 0.0 0.0 3.0010368870132327 0.0 84-85 0.0 0.0 0.0 3.416397350608323 0.0 86-87 0.0 0.0 0.0 3.9645181140285 0.0 88-89 0.0 0.0 0.0 4.633528274552177 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1425 0.0 48.363613 1 GTATCAA 2375 0.0 36.422806 1 TCGTATA 60 0.0039453083 31.67115 2 CATATAG 100 4.940752E-5 28.504034 3 ATCAACG 3090 0.0 27.981308 3 TCAACGC 3155 0.0 27.404829 4 CAACGCA 3175 0.0 27.232203 5 TATCAAC 3180 0.0 27.189383 2 TCGTAGC 70 0.008379658 27.146702 7 TAGTACA 70 0.008379658 27.146702 4 AACGCAG 3220 0.0 26.845121 6 TATTCGT 90 8.930409E-4 26.392624 4 GTATAGA 175 1.4723992E-7 24.443878 1 TAGAGTG 100 0.0016504957 23.753363 5 ACGCAGA 3690 0.0 23.431501 7 CGCAGAG 3680 0.0 23.366077 8 TTCGTAG 105 0.002195139 22.61677 6 GAGTACT 2495 0.0 22.372908 12-13 GCAGAGT 3845 0.0 22.239817 9 AGTACTT 2600 0.0 21.92618 12-13 >>END_MODULE