FastQCFastQC Report
Thu 26 May 2016
SRR937273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444629
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.5332535664565289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19810.44553998951935203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.27326152815043553No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6110.13741793720157705No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.10660573196979953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG255.266862E-576.014251
CCATGCG504.233807E-757.004269
GGTATCA11350.044.3695531
GTATCAA21400.042.402811
ATCAACG27000.033.6081543
TCAACGC27100.033.491694
TATCAAC27950.032.8057942
CAACGCA28050.032.3610425
AACGCAG28500.031.8393026
CGCAGAG31450.028.248548
ACGCAGA31750.028.131267
ATAGTAC856.39391E-427.9464173
GTAATAC1205.364076E-627.713533
GCAGAGT33100.026.4068099
GTACATG20300.024.3395391
TAGTACA1000.00164808223.7598134
GTTGCCG500.001651545523.75445412-13
CATGGGA11250.023.6542154
ACATGGG19950.023.575853
AGTACTT19700.023.51329212-13
Produced by FastQC (version 0.11.5)