##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937273_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 444629 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775986271700678 33.0 31.0 34.0 30.0 34.0 2 31.874191741879184 33.0 31.0 34.0 30.0 34.0 3 31.907100076693155 33.0 31.0 34.0 30.0 34.0 4 35.54522309610934 37.0 35.0 37.0 33.0 37.0 5 35.51789019609607 37.0 35.0 37.0 33.0 37.0 6 35.5942842234762 37.0 35.0 37.0 33.0 37.0 7 35.55061635655794 37.0 35.0 37.0 33.0 37.0 8 35.54543225925435 37.0 35.0 37.0 33.0 37.0 9 37.168715940705624 39.0 37.0 39.0 34.0 39.0 10-11 37.084893248078735 39.0 37.0 39.0 33.0 39.0 12-13 36.98627507427541 39.0 37.0 39.0 33.0 39.0 14-15 38.159832129708136 40.0 38.0 41.0 33.0 41.0 16-17 38.10711177183674 40.0 38.0 41.0 33.0 41.0 18-19 38.09211050111441 40.0 38.0 41.0 33.5 41.0 20-21 38.035193385946485 40.0 38.0 41.0 33.0 41.0 22-23 37.90561569308345 40.0 38.0 41.0 33.0 41.0 24-25 37.77339422304888 40.0 37.5 41.0 33.0 41.0 26-27 37.60823068220921 40.0 37.0 41.0 32.0 41.0 28-29 37.435690204642526 40.0 37.0 41.0 32.0 41.0 30-31 37.404990452714515 39.5 37.0 41.0 32.0 41.0 32-33 37.256315939806 40.0 36.5 41.0 32.0 41.0 34-35 37.073267825535446 39.0 36.0 41.0 31.0 41.0 36-37 37.07223100607473 39.5 36.0 41.0 31.0 41.0 38-39 37.060418911047186 40.0 36.0 41.0 31.0 41.0 40-41 36.91140928729345 40.0 36.0 41.0 31.0 41.0 42-43 36.68309646919117 39.5 36.0 41.0 30.5 41.0 44-45 36.44260158469196 39.0 36.0 41.0 30.0 41.0 46-47 36.083736103582986 39.0 35.5 41.0 29.5 41.0 48-49 35.77650355689799 39.0 35.0 41.0 28.0 41.0 50-51 34.84807333754658 38.0 34.0 40.0 26.0 40.5 52-53 34.97801313004775 38.5 35.0 40.0 27.0 40.5 54-55 35.358717042748 39.0 35.0 40.0 27.0 41.0 56-57 35.16261534897634 39.0 35.0 40.0 26.0 41.0 58-59 34.94546577033887 38.5 35.0 40.0 26.0 41.0 60-61 34.68096660361785 38.0 34.0 40.0 25.5 41.0 62-63 34.3817148679011 38.0 34.0 40.0 25.0 41.0 64-65 34.03779892899473 37.5 34.0 40.0 23.0 41.0 66-67 33.65584228649053 37.0 33.5 40.0 22.5 41.0 68-69 33.19108627642372 36.0 33.0 39.0 21.5 41.0 70-71 32.78041693186904 36.0 32.5 39.0 20.0 40.0 72-73 32.375390494097324 35.5 32.0 38.5 19.0 40.0 74-75 31.929230886874226 35.0 32.0 37.5 18.0 39.5 76-77 31.4839754042134 35.0 31.0 37.0 14.5 39.0 78-79 31.023751262288336 35.0 31.0 36.5 9.5 39.0 80-81 30.57883538860488 35.0 31.0 36.0 7.0 38.5 82-83 30.148726241428246 34.0 30.0 35.5 4.0 37.0 84-85 29.694373286492784 34.0 30.0 35.0 2.0 37.0 86-87 29.274849368799607 34.0 29.0 35.0 2.0 36.0 88-89 28.909635898693068 34.0 29.0 35.0 2.0 36.0 90-91 28.500039358656316 34.0 29.0 35.0 2.0 36.0 92-93 28.090007624333996 34.0 28.5 35.0 2.0 35.0 94-95 27.59477789347973 34.0 27.0 35.0 2.0 35.0 96-97 27.01881006412087 33.0 26.0 35.0 2.0 35.0 98-99 26.368154124000007 33.0 25.0 35.0 2.0 35.0 100-101 24.567070523964922 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12622852577235122 1101 2 0.0746858908011454 1101 3 0.3168507707359822 1101 4 0.0950332932689264 1101 5 0.24307413589239246 1101 6 0.09872311676700463 1101 7 0.1940286605567394 1101 8 0.09888571285265613 1101 9 0.20761216424763518 1101 10-11 0.11807172494886942 1101 12-13 0.22620854716187466 1101 14-15 0.5287595581392495 1101 16-17 0.3811835043005516 1101 18-19 0.3481753721152643 1101 20-21 0.2738692216705303 1101 22-23 0.12046451351976373 1101 24-25 0.2138270029049636 1101 26-27 0.5778038655731876 1101 28-29 0.29683127067045945 1101 30-31 0.3292374437523051 1101 32-33 0.47724365499413324 1101 34-35 0.26186769572912283 1101 36-37 0.39391460815547674 1101 38-39 0.3014217749284285 1101 40-41 0.323578738911273 1101 42-43 0.5421608671054301 1101 44-45 0.507515452364494 1101 46-47 0.5424414510163658 1101 48-49 0.7496803401184877 1101 50-51 0.6735863620727756 1101 52-53 0.5759316754677997 1101 54-55 0.504493168721666 1101 56-57 0.6168559085278744 1101 58-59 0.4364894123308005 1101 60-61 0.6211531108059702 1101 62-63 0.7113055708296798 1101 64-65 0.65549651991752 1101 66-67 0.7753589091637991 1101 68-69 0.3395049964786665 1101 70-71 0.7847354944877267 1101 72-73 1.0378212959995636 1101 74-75 0.7058555826745874 1101 76-77 0.7232658436007711 1101 78-79 0.6879017393880069 1101 80-81 0.89195523380452 1101 82-83 0.8427384174984205 1101 84-85 0.8938568703996452 1101 86-87 1.1769306754145603 1101 88-89 1.1538774073587135 1101 90-91 1.120335298700315 1101 92-93 1.1714175465581391 1101 94-95 1.1643684561781242 1101 96-97 1.3188164118808672 1101 98-99 1.418137276827217 1101 100-101 1.6749773445348808 1102 1 -0.028059714722786566 1102 2 -0.005799384312776823 1102 3 -0.08607720314689615 1102 4 -0.2298408820694391 1102 5 -0.1443723036097495 1102 6 0.01722614336021877 1102 7 -0.1180590086264175 1102 8 0.07161236727692 1102 9 0.15370309807066462 1102 10-11 -0.10383026784171534 1102 12-13 0.0966346506781619 1102 14-15 0.1674174850929404 1102 16-17 0.1945761792648497 1102 18-19 0.2058362236421658 1102 20-21 0.39057159533576424 1102 22-23 0.37490663417541015 1102 24-25 0.5201420406523596 1102 26-27 0.5485198759112109 1102 28-29 0.3489804919178283 1102 30-31 0.25704979497314895 1102 32-33 0.25758610211376975 1102 34-35 0.390072823934247 1102 36-37 0.055361251697611635 1102 38-39 0.26525993720823493 1102 40-41 0.3668267155146836 1102 42-43 0.47010499415432605 1102 44-45 0.6374846763323916 1102 46-47 0.530339329000455 1102 48-49 0.4870341114576675 1102 50-51 0.35795349803142784 1102 52-53 0.30673916373549304 1102 54-55 0.37510205806397323 1102 56-57 0.05957593165239672 1102 58-59 0.24605208661496647 1102 60-61 0.5486849070380302 1102 62-63 0.5520991163646087 1102 64-65 0.5617741503330862 1102 66-67 0.6862827002268546 1102 68-69 0.6495225099113 1102 70-71 0.532601576239756 1102 72-73 0.39282537679940077 1102 74-75 0.3493594594344245 1102 76-77 0.08246549673279091 1102 78-79 0.40840520806786884 1102 80-81 0.2765536150872485 1102 82-83 0.49202767819351934 1102 84-85 0.6753045341417412 1102 86-87 0.44922204452290515 1102 88-89 0.36209596819664114 1102 90-91 0.1268135684411824 1102 92-93 0.03634732279428121 1102 94-95 0.03555545883064681 1102 96-97 0.21023738039069784 1102 98-99 0.41638168273077625 1102 100-101 0.6956363937085221 1103 1 -0.1752376142226666 1103 2 -0.31311614471360727 1103 3 -0.28368835598548614 1103 4 -0.18830626760079383 1103 5 -0.1706789333933969 1103 6 -0.26850580747056796 1103 7 -0.1301635390091107 1103 8 0.044563802600350755 1103 9 0.12540423570416692 1103 10-11 -0.1714242004630364 1103 12-13 -0.01021542351532645 1103 14-15 0.028112339179074297 1103 16-17 -0.34732482280524124 1103 18-19 -0.4653744125899024 1103 20-21 -0.36974221564486953 1103 22-23 -0.23285253925416072 1103 24-25 -0.4116800964865206 1103 26-27 -0.6141310299821328 1103 28-29 -0.7362458440229673 1103 30-31 -0.5531110491838902 1103 32-33 -0.7460090762253984 1103 34-35 -0.6829207459633508 1103 36-37 -0.5444060921754783 1103 38-39 -0.4576548593550456 1103 40-41 -0.44602020774543405 1103 42-43 -0.4365921132155606 1103 44-45 -0.7252275994841924 1103 46-47 -1.003150787693997 1103 48-49 -0.9509731063780791 1103 50-51 -1.0619702017505759 1103 52-53 -0.9019094155965774 1103 54-55 -1.0112513777357535 1103 56-57 -0.5898827306664671 1103 58-59 -0.6329209289381907 1103 60-61 -0.6771770197943638 1103 62-63 -0.6854574447213722 1103 64-65 -0.544798843236812 1103 66-67 -0.3914968077146028 1103 68-69 -0.5441179172966386 1103 70-71 -0.5068377591331412 1103 72-73 -0.4831432604219188 1103 74-75 -0.5081203434490433 1103 76-77 -0.6361808842736778 1103 78-79 -0.43260776205322315 1103 80-81 -0.28688181825768666 1103 82-83 -0.3638423091661096 1103 84-85 -0.32573051083234006 1103 86-87 -0.3258984054010696 1103 88-89 -0.2038789892108035 1103 90-91 -0.2381039027515932 1103 92-93 -0.3754379923906548 1103 94-95 -0.347541707161259 1103 96-97 -0.23806990889063329 1103 98-99 -0.26253912258917467 1103 100-101 -0.2458217373483258 1104 1 -0.07686173237726379 1104 2 -0.04999377756139367 1104 3 0.03745563223965931 1104 4 0.04463287872209776 1104 5 0.02155067631071006 1104 6 0.059058522834462224 1104 7 -0.0426540226917993 1104 8 -0.22681619029413724 1104 9 -0.08829418585600735 1104 10-11 -0.04229665643421043 1104 12-13 -0.029029817262468782 1104 14-15 0.09662982382608476 1104 16-17 -0.1635380898204204 1104 18-19 -0.07272862260868607 1104 20-21 0.06949292621170144 1104 22-23 0.21745198289982426 1104 24-25 0.08947708769863283 1104 26-27 0.011045339098714635 1104 28-29 0.11287611709970946 1104 30-31 0.0781963659305589 1104 32-33 0.05084927021930952 1104 34-35 0.049845837805627013 1104 36-37 0.02226395807067405 1104 38-39 0.2134459882316122 1104 40-41 0.06466598217487984 1104 42-43 -0.01579325009826249 1104 44-45 0.032588469134800846 1104 46-47 0.07936202761332112 1104 48-49 -0.020008278686866277 1104 50-51 -0.1535146366082074 1104 52-53 -0.1765909361771989 1104 54-55 0.0287323810826976 1104 56-57 -0.18355628291738668 1104 58-59 -0.24230274896559933 1104 60-61 0.03574233258851933 1104 62-63 0.1404345514213574 1104 64-65 0.038886382363344296 1104 66-67 0.17998723024916075 1104 68-69 0.03673271458682592 1104 70-71 -0.059121675288032804 1104 72-73 0.0813063157847509 1104 74-75 0.24435897442133125 1104 76-77 0.4728913382305109 1104 78-79 0.33242548161525676 1104 80-81 0.6859725693990164 1104 82-83 0.5143843568238431 1104 84-85 0.4210897703431158 1104 86-87 0.4636463339787191 1104 88-89 0.4800244772663831 1104 90-91 0.4169435519507374 1104 92-93 0.26629930649783873 1104 94-95 0.5862805534081978 1104 96-97 0.7588696434617965 1104 98-99 0.7164107835164977 1104 100-101 1.0484227939413238 1105 1 0.07675664009357064 1105 2 0.04598909055262013 1105 3 0.08401448617556184 1105 4 -0.01295831903303224 1105 5 0.1824574847585012 1105 6 0.06323072782633687 1105 7 0.08898827534394371 1105 8 -0.2842301616425331 1105 9 -0.07116229987165923 1105 10-11 0.13232304523467064 1105 12-13 0.0499503924554503 1105 14-15 0.43694144692459247 1105 16-17 0.10270027969664142 1105 18-19 0.16824759951507673 1105 20-21 0.06716682962331078 1105 22-23 0.12572624076424432 1105 24-25 0.07546881713297893 1105 26-27 0.13044055398573562 1105 28-29 0.06616957449152494 1105 30-31 0.2358466391546017 1105 32-33 0.21112840180991554 1105 34-35 0.21569652455794852 1105 36-37 0.21291895733223498 1105 38-39 0.22459279077185812 1105 40-41 0.49490523782397844 1105 42-43 0.40129802150119787 1105 44-45 0.3848259525198259 1105 46-47 0.7526193012804256 1105 48-49 0.7323030490343001 1105 50-51 0.5969321216254357 1105 52-53 0.7201340675150121 1105 54-55 0.8134982945371405 1105 56-57 0.8378290368285874 1105 58-59 1.082730480985667 1105 60-61 1.2840919116758087 1105 62-63 0.8960873353528243 1105 64-65 1.1179072064661426 1105 66-67 0.8762248613228536 1105 68-69 0.8820842136268467 1105 70-71 0.8651952646026686 1105 72-73 0.8643590406407569 1105 74-75 0.7993434671107451 1105 76-77 0.8827680181891537 1105 78-79 0.9560556691798681 1105 80-81 1.199737153655402 1105 82-83 0.859824469067231 1105 84-85 0.9822771872682381 1105 86-87 0.9146986157687067 1105 88-89 0.8169909519377754 1105 90-91 0.7788472589364126 1105 92-93 0.5228271458125668 1105 94-95 1.1394189376941242 1105 96-97 0.8554543429153476 1105 98-99 0.7754493302599634 1105 100-101 1.4057555365199725 1106 1 0.052116328139824475 1106 2 0.13513794089550046 1106 3 0.11840335265398139 1106 4 0.19219833015346666 1106 5 0.12806727365187243 1106 6 0.09237554428459305 1106 7 0.19632322223112908 1106 8 -0.2733050574338449 1106 9 -0.2548385133986173 1106 10-11 -0.18736231176411877 1106 12-13 -0.11471613974840267 1106 14-15 -0.26098782563154543 1106 16-17 -0.15738443188060813 1106 18-19 0.031129137769745796 1106 20-21 0.07634391715860289 1106 22-23 0.04681337267226837 1106 24-25 -0.027059083902379655 1106 26-27 0.008398736048391697 1106 28-29 0.05558062733540936 1106 30-31 0.1574884301993862 1106 32-33 0.2914699373753322 1106 34-35 0.31161893951021824 1106 36-37 -0.1749222420761285 1106 38-39 -0.24658174196984106 1106 40-41 -0.20773023346821873 1106 42-43 -0.19324614953550423 1106 44-45 -0.39290700431761394 1106 46-47 -0.3679098099203273 1106 48-49 -0.3819567331359295 1106 50-51 -0.6994407437772807 1106 52-53 -0.537177348378485 1106 54-55 -0.4101469925409873 1106 56-57 -0.4066579875091847 1106 58-59 -0.7548384312101106 1106 60-61 -0.7934763282058626 1106 62-63 -0.4966953727283894 1106 64-65 -0.4717122637503479 1106 66-67 -0.1983179650856357 1106 68-69 -0.44020636052939466 1106 70-71 -0.7504763771883738 1106 72-73 -0.13221215742297687 1106 74-75 -0.46659037718130847 1106 76-77 -0.7262645218135333 1106 78-79 -0.4912349049763769 1106 80-81 -0.5746342326562797 1106 82-83 -0.5894326238465801 1106 84-85 -0.529521925277141 1106 86-87 -0.6541295407460197 1106 88-89 -0.56649701363974 1106 90-91 -0.6302222583420622 1106 92-93 -0.4124626215667533 1106 94-95 -0.5429462633277922 1106 96-97 -0.54317749788067 1106 98-99 -0.4487674238075421 1106 100-101 -0.21670647773308716 1107 1 -0.07873415876215972 1107 2 -0.02789759233880318 1107 3 0.17136131167404756 1107 4 0.04213113060617957 1107 5 0.33236568679939893 1107 6 0.1829416709969962 1107 7 -0.028972102095110586 1107 8 0.17901863217498004 1107 9 0.1780798332567315 1107 10-11 0.10674770686312485 1107 12-13 0.1433654900174659 1107 14-15 0.3647519588583279 1107 16-17 0.1350750715500979 1107 18-19 0.13198740567194278 1107 20-21 0.4189024515011113 1107 22-23 0.2730459538422494 1107 24-25 0.3242412862845967 1107 26-27 0.48158375880192494 1107 28-29 0.46807384843371125 1107 30-31 0.16752120471542753 1107 32-33 0.002853776473678238 1107 34-35 0.11573815386498154 1107 36-37 -0.15184450281036987 1107 38-39 -0.038274934773589564 1107 40-41 0.2659440491471443 1107 42-43 0.2911564818572927 1107 44-45 0.543465634856183 1107 46-47 0.7633415163865251 1107 48-49 0.7061513048730816 1107 50-51 0.5821430434477364 1107 52-53 0.731155159697245 1107 54-55 0.8641686331221621 1107 56-57 0.9836063307101384 1107 58-59 0.6789582253176789 1107 60-61 1.0780428531385908 1107 62-63 1.0241188028392685 1107 64-65 0.8289998156630105 1107 66-67 1.181276951327554 1107 68-69 1.1629272604651604 1107 70-71 1.068591789089247 1107 72-73 0.8652236493252659 1107 74-75 0.9609932685709737 1107 76-77 0.6035260985443927 1107 78-79 1.0621356828428183 1107 80-81 0.9818664393391927 1107 82-83 1.1966429225918453 1107 84-85 1.1546225183813412 1107 86-87 1.1007335843866102 1107 88-89 1.1672748851600971 1107 90-91 1.6046873170039575 1107 92-93 1.3090659912931173 1107 94-95 1.2597169608357497 1107 96-97 1.2699488136894423 1107 98-99 1.4399171155808297 1107 100-101 1.3445924777265823 1108 1 0.09002869949919656 1108 2 0.10115145854451413 1108 3 -0.029385820985730504 1108 4 -0.14812116201661496 1108 5 0.05115394923704031 1108 6 0.028979868883041604 1108 7 0.09988537631134164 1108 8 -0.023914925492498185 1108 9 0.012515402435219869 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2316 46-47 -0.6775608788997474 2316 48-49 -0.388192446461197 2316 50-51 -0.5280678783745643 2316 52-53 -0.6537398063588569 2316 54-55 -0.6065467505079098 2316 56-57 -0.6531619541624067 2316 58-59 -0.5141163658611845 2316 60-61 -0.6629801603962662 2316 62-63 -0.8854441961787032 2316 64-65 -1.0859216223626902 2316 66-67 -1.0077841476861522 2316 68-69 -1.0266995701569357 2316 70-71 -1.159579510172101 2316 72-73 -1.1896950134132993 2316 74-75 -1.5080889653216865 2316 76-77 -1.1769166664955257 2316 78-79 -0.8333587452345448 2316 80-81 -1.0558934769752248 2316 82-83 -0.9870223777796134 2316 84-85 -0.9501552451641935 2316 86-87 -0.8769680508979611 2316 88-89 -0.9273784174315942 2316 90-91 -1.0342158041266494 2316 92-93 -1.1990197010342847 2316 94-95 -1.2130585397833507 2316 96-97 -1.0690952066341985 2316 98-99 -1.0477488679382354 2316 100-101 -1.4219471661827292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1693.0 3 364.0 4 263.0 5 309.0 6 330.0 7 406.0 8 427.0 9 618.0 10 783.0 11 1016.0 12 1427.0 13 1864.0 14 2142.0 15 2654.0 16 3490.0 17 4835.0 18 4093.0 19 2608.0 20 2476.0 21 2549.0 22 2812.0 23 3027.0 24 3425.0 25 3970.0 26 4423.0 27 5315.0 28 6459.0 29 7602.0 30 9484.0 31 11788.0 32 15131.0 33 20230.0 34 27944.0 35 40542.0 36 60549.0 37 91366.0 38 83994.0 39 12220.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44065029283023 20.47015206424449 15.48403246502838 21.605165177896897 2 14.74280354952745 20.95763292691725 41.28535098477744 23.01421253877787 3 18.388241554084523 26.13205930628957 30.686901390667558 24.792797748958353 4 11.931811776695067 17.568320117600113 39.06461016781012 31.435257937894693 5 12.75694466362173 38.47282777775273 34.84099752467436 13.929230033951187 6 29.635479924017943 38.34998163541683 18.193781278941117 13.820757161624107 7 27.3341671455453 34.05852460309412 21.381649370697133 17.225658880663445 8 25.99121622991935 35.546101272497424 20.154448332818806 18.308234164764418 9 26.23463188635708 16.995285910008654 21.038992644937988 35.731089558696276 10-11 23.945674354874512 27.83778027929052 27.955306305727674 20.261239060107293 12-13 25.185641751421468 25.318154467458886 28.56426528685172 20.931938494267925 14-15 22.378215650994342 26.509706807214727 27.034846711200096 24.07723083059084 16-17 20.928023580871642 30.09107615895905 27.655017076075577 21.325883184093726 18-19 21.003574860830543 28.18957465578022 30.637447371356274 20.16940311203296 20-21 21.77455699168965 27.38156400411011 30.599007625331243 20.244871378869 22-23 21.541496632363113 27.103111205251214 30.601217261589557 20.75417490079611 24-25 21.097239888284143 27.36080864434678 30.854464071454885 20.687487395914193 26-27 20.956689275976295 27.580616084908847 30.65157626698515 20.811118372129705 28-29 20.84497552885612 27.65813857066888 30.89155182204115 20.605334078433845 30-31 20.921179535252655 27.65014602224586 30.676502136251376 20.752172306250113 32-33 20.823582566135272 27.643805272343574 30.542501326705253 20.9901108348159 34-35 21.2289558685848 27.58937312908194 30.394919327308838 20.78675167502442 36-37 21.226679225342988 27.404804369698287 30.38531175674625 20.98320464821247 38-39 20.981050449516687 27.789707307181 30.415267794777044 20.81397444852527 40-41 21.178425573862835 27.62754541613857 30.442022250387268 20.752006759611323 42-43 20.8625726217742 27.680147626252328 30.193083511801426 21.26419624017205 44-45 21.15526752235362 27.839270262474763 30.04804225555235 20.957419959619266 46-47 21.197581449215278 28.249322078006937 29.412632838349822 21.14046363442796 48-49 21.244793550255125 28.29075226368605 29.211436219652747 21.253017966406077 50-51 21.456441180016384 28.384186978267067 29.063812958637687 21.09555888307887 52-53 21.46198006749334 28.62303449596899 28.822021532515674 21.092963904021993 54-55 21.308449756786814 28.55740834012212 28.888115173866158 21.246026729224905 56-57 21.241865039355353 28.920411724482104 28.787706394526865 21.050016841635678 58-59 21.34631480173522 28.389133145663024 29.06986044132937 21.194691611272383 60-61 21.293081500273747 28.506282683585717 29.15877153652563 21.041864279614906 62-63 21.274186861813824 28.510643571048767 29.348790994871983 20.866378572265432 64-65 21.17389154182114 28.433688522097068 29.17639690119489 21.216023034886906 66-67 21.044282583836097 28.696533892146757 28.85415082329216 21.405032700724995 68-69 21.101829999831025 28.475225440884078 29.106516241318907 21.31642831796599 70-71 21.128248920453203 28.530565848130045 29.077070572449898 21.264114658966857 72-73 21.1215841976822 28.590211882834627 28.847432190958195 21.440771728524975 74-75 21.071220154071124 28.760468747535604 28.743006752722383 21.425304345670888 76-77 21.125541905886383 28.538498977025895 29.004132454867467 21.33182666222026 78-79 21.127920522578915 28.696662227720203 28.672214223105506 21.503203026595376 80-81 21.024694181248375 28.74206234320417 28.670303785404737 21.56293969014272 82-83 21.097964370856747 28.873869407878427 28.620711411150218 21.407454810114604 84-85 21.175752277786287 28.585879978010688 28.569771004749335 21.66859673945369 86-87 20.997099163545215 28.586926521530962 28.708142056495902 21.707832258427914 88-89 21.112957465624948 28.27209382234815 28.868059506441273 21.746889205585628 90-91 21.05097137964637 28.400335716015796 28.903121145039457 21.645571759298377 92-93 21.19447041990108 28.260232765128045 28.755506483849523 21.78979033112135 94-95 21.02465596007278 28.324667789525865 28.705914163235185 21.94476208716617 96-97 21.0215328738512 28.354696473673656 28.686578059765722 21.93719259270942 98-99 20.943607514792102 28.232180758779503 28.607897831994194 22.216313894434204 100-101 20.970908783688643 28.279591089582922 28.84620800360472 21.903292123123716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 617.0 1 365.5 2 113.0 3 125.0 4 115.5 5 112.5 6 188.0 7 391.0 8 676.5 9 847.5 10 798.0 11 718.5 12 680.5 13 700.5 14 775.5 15 823.5 16 817.0 17 805.5 18 857.0 19 963.0 20 1060.5 21 1182.0 22 1385.0 23 1629.5 24 1970.5 25 2369.0 26 3034.0 27 3993.0 28 4706.5 29 5345.5 30 6350.5 31 7337.5 32 8327.0 33 9847.0 34 11489.0 35 12597.0 36 14061.0 37 15509.5 38 15895.5 39 16014.0 40 15830.5 41 15746.0 42 15810.5 43 16113.5 44 16595.0 45 16794.5 46 16504.5 47 16200.5 48 16751.0 49 16215.0 50 14792.5 51 13668.0 52 12707.0 53 12166.5 54 11636.5 55 10449.0 56 8944.5 57 7884.5 58 7223.0 59 6399.0 60 5185.5 61 4040.0 62 3113.5 63 2293.0 64 1673.5 65 1249.5 66 873.5 67 627.5 68 483.0 69 370.5 70 275.0 71 204.5 72 145.0 73 110.0 74 88.5 75 53.5 76 36.5 77 20.5 78 15.0 79 18.5 80 11.0 81 7.5 82 5.5 83 2.5 84 1.0 85 0.0 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26943811582240473 2 0.2422244163111268 3 0.24762217489187616 4 0.24559801542409515 5 0.23637684451531502 6 0.18892155032622704 7 0.19881744105760082 8 0.1925200560467266 9 0.1918453362241329 10-11 0.20185368025927233 12-13 0.20196613356303797 14-15 0.18948381684505508 16-17 0.19780536132371035 18-19 0.18790947059233654 20-21 0.19004608336388315 22-23 0.19027098997141437 24-25 0.1854354979094931 26-27 0.19319477586932027 28-29 0.18914645693375826 30-31 0.1936445890843827 32-33 0.1935321357806171 34-35 0.18656003094714918 36-37 0.19015853666764876 38-39 0.18419851156807138 40-41 0.1836362450492433 42-43 0.18116227236639984 44-45 0.1918453362241329 46-47 0.18206189879652473 48-49 0.186335124339618 50-51 0.19240760274296098 52-53 0.19825517453877275 54-55 0.198480081146304 56-57 0.17733886003836907 58-59 0.17216600806515095 60-61 0.1776762199496659 62-63 0.17835093977225958 64-65 0.17553960717811928 66-67 0.18700984416221164 68-69 0.1746399807479944 70-71 0.18183699218899352 72-73 0.19027098997141437 74-75 0.1817245388852279 76-77 0.1855479512132587 78-79 0.18667248425091482 80-81 0.17531470057058807 82-83 0.18779701728857093 84-85 0.17475243405176 86-87 0.17801357986096275 88-89 0.1825117120115872 90-91 0.18026264593627495 92-93 0.1875721106810397 94-95 0.18476077808689942 96-97 0.17902565959485323 98-99 0.1823992587078216 100-101 0.17239091467268217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 444629.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.87101919225226 #Duplication Level Percentage of deduplicated Percentage of total 1 83.98042103383338 55.31875925693065 2 9.378468657705428 12.35538577891301 3 2.6346605026343775 5.206433176022943 4 1.206220459608326 3.1781988417978955 5 0.6437553970161008 2.1202412055981776 6 0.43034150363020546 1.7008220066908775 7 0.29247010407472585 1.3485712687066398 8 0.22321893813802948 1.176292716653145 9 0.16690716214550805 0.989491039291001 >10 0.9785998145796807 11.631380375888504 >50 0.05058205867551998 2.33930319120672 >100 0.012987285295982153 1.244281465484362 >500 3.417706656837409E-4 0.13755309850026146 >1k 0.0010253119970512227 1.253286578315803 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2371 0.5332535664565289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 0.44553998951935203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 0.27326152815043553 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 611 0.13741793720157705 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 474 0.10660573196979953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.2490660753122265E-4 0.0 22-23 0.0 0.0 0.0 4.498132150624453E-4 0.0 24-25 0.0 0.0 0.0 8.996264301248906E-4 0.0 26-27 0.0 0.0 0.0 0.0012369863414217245 0.0 28-29 0.0 0.0 0.0 0.0015743462527185587 0.0 30-31 0.0 0.0 0.0 0.0035985057204995625 0.0 32-33 0.0 0.0 0.0 0.007084558137233514 0.0 34-35 0.0 0.0 0.0 0.008996264301248906 0.0 36-37 0.0 0.0 0.0 0.010907970465264299 0.0 38-39 0.0 0.0 0.0 0.012707223325514081 0.0 40-41 0.0 0.0 0.0 0.016418182349779254 0.0 42-43 0.0 0.0 0.0 0.02440236691713766 0.0 44-45 0.0 0.0 0.0 0.03441071095227707 0.0 46-47 0.0 0.0 0.0 0.047905107404150424 0.0 48-49 0.0 0.0 0.0 0.08400261791291166 0.0 50-51 0.0 0.0 0.0 0.11863823547271996 0.0 52-53 0.0 0.0 0.0 0.15102478695721602 0.0 54-55 0.0 0.0 0.0 0.2002793340065538 0.0 56-57 0.0 0.0 0.0 0.26269091759646807 0.0 58-59 0.0 0.0 0.0 0.3421954033587553 0.0 60-61 0.0 0.0 0.0 0.43924260450847785 0.0 62-63 0.0 0.0 0.0 0.5889179518205065 0.0 64-65 0.0 0.0 0.0 0.7109297864061948 0.0 66-67 0.0 0.0 0.0 0.8803969151809711 0.0 68-69 0.0 0.0 0.0 1.1059782425347873 0.0 70-71 0.0 0.0 0.0 1.2601517219974405 0.0 72-73 0.0 0.0 0.0 1.4763994251387111 0.0 74-75 0.0 0.0 0.0 1.76315534974102 0.0 76-77 0.0 0.0 0.0 2.000094460775163 0.0 78-79 0.0 0.0 0.0 2.215105177575012 0.0 80-81 0.0 0.0 0.0 2.549878662885237 0.0 82-83 0.0 0.0 0.0 2.955722636175328 0.0 84-85 0.0 0.0 0.0 3.347509946494718 0.0 86-87 0.0 0.0 0.0 3.8683936495370297 0.0 88-89 0.0 0.0 0.0 4.480139622021955 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGG 25 5.266862E-5 76.01425 1 CCATGCG 50 4.233807E-7 57.00426 9 GGTATCA 1135 0.0 44.369553 1 GTATCAA 2140 0.0 42.40281 1 ATCAACG 2700 0.0 33.608154 3 TCAACGC 2710 0.0 33.49169 4 TATCAAC 2795 0.0 32.805794 2 CAACGCA 2805 0.0 32.361042 5 AACGCAG 2850 0.0 31.839302 6 CGCAGAG 3145 0.0 28.24854 8 ACGCAGA 3175 0.0 28.13126 7 ATAGTAC 85 6.39391E-4 27.946417 3 GTAATAC 120 5.364076E-6 27.71353 3 GCAGAGT 3310 0.0 26.406809 9 GTACATG 2030 0.0 24.339539 1 TAGTACA 100 0.001648082 23.759813 4 GTTGCCG 50 0.0016515455 23.754454 12-13 CATGGGA 1125 0.0 23.654215 4 ACATGGG 1995 0.0 23.57585 3 AGTACTT 1970 0.0 23.513292 12-13 >>END_MODULE