FastQCFastQC Report
Thu 26 May 2016
SRR937273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444629
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31050.6983350163844463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.6146697583828316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.39785978872273287No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.14753873454048208No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.10210759981917508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.043.9313621
GTATCAA29700.035.0459561
AGGACCG1102.7302685E-630.2247335
GTAAGGT1601.900844E-929.6850034
GTATAGG1951.6370905E-1129.2480491
TAAGGTG1652.6411726E-928.785465
AGCGTCC1004.945413E-528.5008098
ATCAACG36700.027.9540543
GGACCGA1205.3754575E-627.7060036
TCAACGC37100.027.6526624
CAACGCA37300.027.504395
GTGTAAG1754.9130904E-927.1589011
TATCAAC38000.027.122722
AACGCAG38150.026.8915796
GTCCTAC908.9080894E-426.4044881
GGCGCGC908.937401E-426.3896378
GATCGTA651.0015534E-525.5776510-11
CTAGACA1501.0584408E-625.3312034
GAGTACT28150.024.2147212-13
GTACATG18150.024.0913661
Produced by FastQC (version 0.11.5)