FastQCFastQC Report
Thu 26 May 2016
SRR937272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563876
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.5004646411622414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.40558562520837915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.24828153707552725No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7550.13389468606573077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.042.9505461
GTATCAA28700.032.9396361
ACCGTGC753.075825E-431.6677918
GGACCGA856.401753E-427.942176
CAACGCA36850.025.1365785
TCAACGC36900.025.1025184
ATCAACG37050.025.0008893
TATCAAC37300.024.9584542
ATATAGG2701.8189894E-1224.6283172
AACGCAG38150.024.5290536
GTACATG23950.024.0008961
GGCCTAA1852.5359805E-723.1089296
CATTCCG1252.2533738E-422.800819
TACATGG25350.022.296642
TACCTGG4500.022.1654852
ACATGGG24500.022.1028253
GAGTACT23500.021.72756412-13
CATGGGA15400.021.5916774
ACGCAGA42450.021.4848637
CGCAGAG42250.021.3617068
Produced by FastQC (version 0.11.5)