##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937272_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 563876 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82277309195639 33.0 31.0 34.0 30.0 34.0 2 31.91909214082529 33.0 31.0 34.0 30.0 34.0 3 31.9541441735417 33.0 31.0 34.0 30.0 34.0 4 35.56368598769942 37.0 35.0 37.0 33.0 37.0 5 35.59075931587796 37.0 35.0 37.0 33.0 37.0 6 35.62829948428378 37.0 35.0 37.0 33.0 37.0 7 35.52255637764331 37.0 35.0 37.0 33.0 37.0 8 35.525546396725524 37.0 35.0 37.0 33.0 37.0 9 37.16277514914626 39.0 37.0 39.0 34.0 39.0 10-11 37.059556533705994 39.0 37.0 39.0 33.0 39.0 12-13 36.921611311706826 39.0 37.0 39.0 33.0 39.0 14-15 38.10048042477424 40.0 38.0 41.0 33.0 41.0 16-17 38.0386237399712 40.0 38.0 41.0 33.0 41.0 18-19 38.03537923231349 40.0 38.0 41.0 33.0 41.0 20-21 37.9381096198455 40.0 38.0 41.0 33.0 41.0 22-23 37.78610279565011 40.0 38.0 41.0 33.0 41.0 24-25 37.72933942923622 40.0 37.5 41.0 32.5 41.0 26-27 37.75216891657031 40.0 38.0 41.0 33.0 41.0 28-29 37.67863413232696 40.0 37.5 41.0 33.0 41.0 30-31 37.46807188105186 40.0 37.0 41.0 32.0 41.0 32-33 37.242553327327286 40.0 36.5 41.0 32.0 41.0 34-35 37.27260780739028 40.0 37.0 41.0 32.0 41.0 36-37 37.15629411430882 40.0 36.5 41.0 31.0 41.0 38-39 37.05036834339465 40.0 36.0 41.0 31.0 41.0 40-41 36.84423259723769 39.5 36.0 41.0 30.5 41.0 42-43 36.583915612652426 39.0 36.0 41.0 30.0 41.0 44-45 36.35064092105357 39.0 36.0 41.0 30.0 41.0 46-47 36.00668941398463 39.0 35.0 41.0 29.5 41.0 48-49 35.67863856592584 39.0 35.0 41.0 27.5 41.0 50-51 34.7075970603466 38.0 34.0 39.5 25.5 40.5 52-53 34.8268422490051 38.0 34.5 39.5 26.0 40.5 54-55 35.224614099553804 39.0 35.0 40.0 26.5 41.0 56-57 35.024564797934296 39.0 35.0 40.0 26.0 41.0 58-59 34.78120189545219 38.0 34.0 40.0 25.5 41.0 60-61 34.491710056821006 38.0 34.0 40.0 24.5 41.0 62-63 34.18550709730508 38.0 34.0 40.0 23.5 41.0 64-65 33.76939610836425 37.0 33.5 40.0 22.0 41.0 66-67 33.4255421404706 37.0 33.0 39.5 22.0 41.0 68-69 32.971067220452724 36.0 32.5 39.0 20.5 41.0 70-71 32.51419815704162 36.0 32.0 39.0 18.5 40.0 72-73 32.06044946051969 35.0 31.5 38.0 17.5 40.0 74-75 31.577015159361277 35.0 31.0 37.5 14.0 39.5 76-77 31.101310394483892 35.0 31.0 37.0 9.5 39.0 78-79 30.596548709290694 34.5 30.5 36.0 7.0 39.0 80-81 30.098641367960333 34.0 30.0 36.0 3.5 37.5 82-83 29.629097532081524 34.0 29.5 35.5 2.0 37.0 84-85 29.106568642751242 34.0 29.0 35.0 2.0 37.0 86-87 28.661903503607178 34.0 29.0 35.0 2.0 36.0 88-89 28.206158800871115 34.0 28.0 35.0 2.0 36.0 90-91 27.715393987330547 33.5 26.5 35.0 2.0 35.5 92-93 27.215300881754146 33.0 26.0 35.0 2.0 35.0 94-95 26.686267016152485 33.0 25.0 35.0 2.0 35.0 96-97 25.979832445431263 32.5 24.5 35.0 2.0 35.0 98-99 25.097765288822366 32.0 19.0 34.0 2.0 35.0 100-101 22.530374585901868 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.29537355962220246 1101 2 -0.24115425440430016 1101 3 -0.2551622773727651 1101 4 -0.3069261979200846 1101 5 -0.3908971523489271 1101 6 -0.33482356439067473 1101 7 -0.25269795197723965 1101 8 -0.5295449272230925 1101 9 -0.5882334869912 1101 10-11 -0.5232185952162496 1101 12-13 -0.4757731498993465 1101 14-15 -0.7124228280178286 1101 16-17 -0.6341181913583327 1101 18-19 -0.35151914330196377 1101 20-21 -0.3696808177271862 1101 22-23 -0.6089947111911798 1101 24-25 -0.4911288635832989 1101 26-27 -0.3346304233780515 1101 28-29 -0.5273280841181602 1101 30-31 -0.5227975532966624 1101 32-33 -0.6880684868443439 1101 34-35 -0.6970345528709601 1101 36-37 -0.5949961187903909 1101 38-39 -0.6541790733007389 1101 40-41 -0.5629388373286375 1101 42-43 -0.6161416463387539 1101 44-45 -0.47522279423257174 1101 46-47 -0.4309770255989207 1101 48-49 -0.38192387384436444 1101 50-51 -0.4445064194303754 1101 52-53 -0.37971335317400445 1101 54-55 -0.3130192266173495 1101 56-57 -0.20441881576746113 1101 58-59 -0.5905546250573437 1101 60-61 -0.37886609534678684 1101 62-63 -0.3940100423995858 1101 64-65 -0.6397313822735597 1101 66-67 -0.6526086875961568 1101 68-69 -0.5483104984877798 1101 70-71 -0.34550985320189653 1101 72-73 -0.35424312277201864 1101 74-75 -0.400535408261149 1101 76-77 -0.16390732196682123 1101 78-79 -0.4882179492429124 1101 80-81 -0.6696729139954662 1101 82-83 -0.4741845753643332 1101 84-85 -0.7425553335363695 1101 86-87 -0.7523256275818291 1101 88-89 -0.7882657952338903 1101 90-91 -0.5087470341821572 1101 92-93 -0.3372973466687874 1101 94-95 -0.9214950229066652 1101 96-97 -0.8860963579110326 1101 98-99 -0.4345301597073785 1101 100-101 0.15550714347340389 1102 1 -0.22278518630033162 1102 2 -0.3323696133458185 1102 3 -0.14004484929690264 1102 4 -0.20346649543837714 1102 5 -0.24343513324876653 1102 6 -0.16063236574959916 1102 7 -0.2127915123152988 1102 8 -0.15354073581547567 1102 9 -0.2752034037899662 1102 10-11 -0.23942984932481437 1102 12-13 -0.20531316978166814 1102 14-15 -0.2838230387823444 1102 16-17 -0.3111365004059863 1102 18-19 -0.3390068661197674 1102 20-21 -0.4382031674231328 1102 22-23 -0.4181990900777066 1102 24-25 -0.2448762029052034 1102 26-27 -0.2284182438162432 1102 28-29 -0.4439984824511498 1102 30-31 -0.2536078323954527 1102 32-33 -0.16504186662946552 1102 34-35 -0.06839804403885097 1102 36-37 -0.19587574717613165 1102 38-39 -0.001503916346848655 1102 40-41 -0.10118692447647248 1102 42-43 -0.0514166402934606 1102 44-45 -0.1563981795644338 1102 46-47 -0.12279064528540573 1102 48-49 -0.4915870984220163 1102 50-51 -0.09927504119166741 1102 52-53 -0.15619101840472638 1102 54-55 -0.08705190407300734 1102 56-57 -0.10715367938251319 1102 58-59 -0.15188978541451803 1102 60-61 -0.011516384476294661 1102 62-63 -0.23137730702846682 1102 64-65 -0.16920184389560688 1102 66-67 -0.20547072639951125 1102 68-69 -0.08852592012128468 1102 70-71 -0.12960683473163925 1102 72-73 -0.041339169892516736 1102 74-75 0.024754392945524728 1102 76-77 0.03027665216825426 1102 78-79 0.21002159985238222 1102 80-81 0.08311538708134592 1102 82-83 0.001131944573213417 1102 84-85 0.16107886166261665 1102 86-87 0.36613985151934614 1102 88-89 -0.4128168757751922 1102 90-91 -0.19535199401450143 1102 92-93 -0.3793987524621265 1102 94-95 -0.07704505559605934 1102 96-97 -0.25695831312506456 1102 98-99 -0.35495461436165954 1102 100-101 -0.20818702706673164 1103 1 0.05399528513662588 1103 2 -0.08648233692419183 1103 3 0.07308510810786473 1103 4 -0.03907872036358384 1103 5 0.05861353902891153 1103 6 -0.1022573226417336 1103 7 0.20166849756456173 1103 8 -0.06302819492125877 1103 9 -0.17715514161956492 1103 10-11 -0.010541053642128873 1103 12-13 -0.058744090006477734 1103 14-15 -0.2540809403379569 1103 16-17 -0.11911075685352301 1103 18-19 -0.37304810054267534 1103 20-21 -0.4023350914787187 1103 22-23 -0.22778814394157365 1103 24-25 -0.08818959011172467 1103 26-27 -0.18209709253326167 1103 28-29 -0.15761078194130818 1103 30-31 -0.10603445581444504 1103 32-33 -0.22557696342410338 1103 34-35 -0.3233775852779317 1103 36-37 -0.16070438757729022 1103 38-39 -0.38040110058754095 1103 40-41 -0.34804587553390576 1103 42-43 -0.1044532982919506 1103 44-45 -0.1403061291212424 1103 46-47 0.11743435448158124 1103 48-49 0.05325906474475772 1103 50-51 -0.049880415461167615 1103 52-53 -0.07064636797458235 1103 54-55 0.0646505071713861 1103 56-57 -0.05890315975795346 1103 58-59 -0.2763410339115637 1103 60-61 -0.05253177281992549 1103 62-63 -0.08202369202599158 1103 64-65 -0.11382571027520072 1103 66-67 -0.035676991720279716 1103 68-69 0.18892330580121808 1103 70-71 0.12473657419192108 1103 72-73 0.05658792421778003 1103 74-75 0.05748608025736246 1103 76-77 -0.1679449735342864 1103 78-79 -0.11603232925498119 1103 80-81 -0.07753015906038385 1103 82-83 -0.33372064982136607 1103 84-85 -0.6088975571687634 1103 86-87 -0.31370384168312526 1103 88-89 -0.08504096212141832 1103 90-91 -0.13337453127368093 1103 92-93 -0.24646573975812203 1103 94-95 -0.5847835003574069 1103 96-97 -0.6334880598838204 1103 98-99 -0.5681381653314688 1103 100-101 0.22382371028751535 1104 1 0.05300043159054724 1104 2 0.025897850184801285 1104 3 -0.022860863691864353 1104 4 -0.1285677100132787 1104 5 -0.005468421597839779 1104 6 -0.10957523574510475 1104 7 -0.09563965832133903 1104 8 -0.08712469571522519 1104 9 0.2057104192291206 1104 10-11 0.10778433999463743 1104 12-13 0.049747558963268546 1104 14-15 0.026343402963050266 1104 16-17 0.07898945550945058 1104 18-19 0.10844188396056609 1104 20-21 0.1652766860567212 1104 22-23 -0.15189229745512733 1104 24-25 0.11398569543037951 1104 26-27 0.17695416155616783 1104 28-29 0.24342520497253162 1104 30-31 0.12466155903183562 1104 32-33 0.09554549922106048 1104 34-35 0.07982507786848458 1104 36-37 0.3246884839144144 1104 38-39 0.33852885789644915 1104 40-41 0.3581183900919953 1104 42-43 0.1555430666662474 1104 44-45 0.12521932573728378 1104 46-47 0.11527246119875656 1104 48-49 0.10402051034348858 1104 50-51 -0.13421816824005361 1104 52-53 -0.08810413452837196 1104 54-55 0.03554625799883837 1104 56-57 0.1668807657045548 1104 58-59 0.3244452830258169 1104 60-61 0.24495813515612497 1104 62-63 0.30032052730094705 1104 64-65 0.38749438385325163 1104 66-67 0.35789281064823797 1104 68-69 0.46262760471644526 1104 70-71 0.5135234268196669 1104 72-73 0.3376531827840523 1104 74-75 0.40303985017834876 1104 76-77 0.4310381713508278 1104 78-79 0.4194715066707104 1104 80-81 0.5255998032499711 1104 82-83 0.7304713186494283 1104 84-85 0.6465071760383019 1104 86-87 0.5898240620456612 1104 88-89 0.013923050964734784 1104 90-91 0.2708134195193317 1104 92-93 0.6052102058310425 1104 94-95 0.5470632921964977 1104 96-97 0.42244853733441445 1104 98-99 0.4997462403391921 1104 100-101 0.9766982630817118 1105 1 -0.145096341634126 1105 2 -0.1342828946199539 1105 3 -0.1590752345057176 1105 4 0.03908677907228508 1105 5 -0.16677381557546056 1105 6 -0.04906276949292021 1105 7 0.11909157722425334 1105 8 0.017088900859434375 1105 9 0.02974287053047675 1105 10-11 0.01494405018890177 1105 12-13 -0.0069988343168674305 1105 14-15 -0.26109957198431033 1105 16-17 -0.13408400710848412 1105 18-19 -0.05003603520486166 1105 20-21 -0.12463834786630201 1105 22-23 -0.1662891417629666 1105 24-25 -0.13376834346179578 1105 26-27 -0.09985136757958912 1105 28-29 -0.12192892602740102 1105 30-31 -0.04975834558990755 1105 32-33 -0.04764203018834934 1105 34-35 -0.1266898779631731 1105 36-37 -0.21674332463491908 1105 38-39 0.00927780082634655 1105 40-41 0.10322266209023923 1105 42-43 -0.029797114679261938 1105 44-45 0.24200346317808652 1105 46-47 -0.008355516868917334 1105 48-49 -0.24470666712081623 1105 50-51 -0.5455469228829841 1105 52-53 -0.4487427483732276 1105 54-55 -0.3216808644010385 1105 56-57 -0.2539924893371719 1105 58-59 -0.43989649685664034 1105 60-61 -0.2984142772330358 1105 62-63 -0.19846555241231556 1105 64-65 0.022895302928567673 1105 66-67 -0.1609992931131643 1105 68-69 -0.4095077936997029 1105 70-71 -0.32786182726093926 1105 72-73 -0.1977565799717098 1105 74-75 -0.24398049696113944 1105 76-77 -0.12323783157259172 1105 78-79 -0.15865573553600498 1105 80-81 0.08032383036169222 1105 82-83 0.1915365983600239 1105 84-85 0.028923770120044878 1105 86-87 -0.061987724411157075 1105 88-89 0.11385330269258986 1105 90-91 0.12250990440665177 1105 92-93 0.01853546304865361 1105 94-95 0.10313472911102295 1105 96-97 0.052396409678390654 1105 98-99 0.3463634491401564 1105 100-101 0.7320587014612876 1106 1 -0.15781252455347783 1106 2 -0.05210507501676531 1106 3 -0.05903267151053271 1106 4 0.10985459207625325 1106 5 -0.1546941251529219 1106 6 0.028647888046783976 1106 7 -0.4285614055045457 1106 8 -0.21114442245142584 1106 9 -0.314835814390122 1106 10-11 -0.7747225136497349 1106 12-13 -0.29602890565869444 1106 14-15 -0.3402218371166228 1106 16-17 -0.12970860930067118 1106 18-19 -0.45000518679550083 1106 20-21 -0.41323888439433176 1106 22-23 -0.2726009606086066 1106 24-25 -0.33805900576543024 1106 26-27 -0.7038934854205152 1106 28-29 -0.2459268408311246 1106 30-31 -0.4559762525058062 1106 32-33 -0.2757474692705486 1106 34-35 0.018436268601909944 1106 36-37 -0.22714197199074704 1106 38-39 -0.4599997216168461 1106 40-41 -0.2810840882446044 1106 42-43 -0.5176520178191595 1106 44-45 0.018969463545140286 1106 46-47 -0.054923924192877394 1106 48-49 -0.0018232780130347237 1106 50-51 -0.16481702608849247 1106 52-53 -0.08471589854250539 1106 54-55 -0.21865596309641688 1106 56-57 -0.24001991708746573 1106 58-59 0.0916381919844369 1106 60-61 -0.08396902144505702 1106 62-63 -0.18973525732189955 1106 64-65 -0.0846904771261805 1106 66-67 -0.08664779306045034 1106 68-69 -0.25673495123354684 1106 70-71 -0.18149491081326374 1106 72-73 -0.09690278190774038 1106 74-75 0.30184110551610743 1106 76-77 0.1484273187060161 1106 78-79 -0.15128823726390905 1106 80-81 -0.4359077045223927 1106 82-83 -0.23247674064837653 1106 84-85 -0.27318758873286697 1106 86-87 -0.1807111469445566 1106 88-89 0.0174490948133581 1106 90-91 0.15445948482923555 1106 92-93 0.0724137719310427 1106 94-95 0.42716310183790185 1106 96-97 0.5979036707691741 1106 98-99 0.6564631793141977 1106 100-101 1.0018583039463387 1107 1 -0.037920051435197166 1107 2 -0.10865443963064791 1107 3 -0.09671595596800842 1107 4 0.17960043762855094 1107 5 0.03655709674346497 1107 6 0.15352569840846542 1107 7 0.1594640099402227 1107 8 0.05122116894642659 1107 9 -0.036292314878878074 1107 10-11 0.014158229263259159 1107 12-13 -0.094807009275506 1107 14-15 0.21383485887555764 1107 16-17 0.19015942588994506 1107 18-19 -0.13047928259218367 1107 20-21 0.019605690385731123 1107 22-23 0.24807352619490786 1107 24-25 0.229951746922751 1107 26-27 0.15314463774664233 1107 28-29 0.25825204479937724 1107 30-31 0.2587353799477583 1107 32-33 0.16921831763072248 1107 34-35 0.195660120019312 1107 36-37 0.09519429389391121 1107 38-39 0.307229017386085 1107 40-41 0.313392940103391 1107 42-43 0.3890928502160378 1107 44-45 0.5072881341218718 1107 46-47 0.4501836026888455 1107 48-49 0.5561679243330033 1107 50-51 0.6302953883787623 1107 52-53 0.5934121561984469 1107 54-55 0.5989149068938033 1107 56-57 0.47542485319601013 1107 58-59 0.4001914666667332 1107 60-61 0.5252440772841709 1107 62-63 0.6181093174581562 1107 64-65 0.7504757007819407 1107 66-67 0.8564118354830441 1107 68-69 0.3804106988153251 1107 70-71 0.753304698179889 1107 72-73 0.7239427703894279 1107 74-75 0.6443875375786696 1107 76-77 0.47882810071970283 1107 78-79 0.7949727598035388 1107 80-81 0.9798547588207178 1107 82-83 0.9403482837895538 1107 84-85 0.8686990192827757 1107 86-87 0.8315078168873136 1107 88-89 1.2036416656833495 1107 90-91 1.4890635904010843 1107 92-93 1.5551361570727842 1107 94-95 1.5860585075075022 1107 96-97 1.501447170280418 1107 98-99 1.5817808723738267 1107 100-101 1.5111935923138873 1108 1 -0.1263070414602545 1108 2 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2316 98-99 2.15021261245505 2316 100-101 1.9514934985931767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2188.0 3 500.0 4 395.0 5 392.0 6 447.0 7 536.0 8 671.0 9 806.0 10 1055.0 11 1356.0 12 1836.0 13 2377.0 14 2800.0 15 3527.0 16 4562.0 17 6193.0 18 5255.0 19 3528.0 20 3518.0 21 3566.0 22 3823.0 23 4189.0 24 4809.0 25 5392.0 26 6315.0 27 7448.0 28 8796.0 29 10565.0 30 12994.0 31 16400.0 32 20898.0 33 27176.0 34 37435.0 35 53483.0 36 79107.0 37 114299.0 38 93913.0 39 11326.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39391257052055 20.303696040895694 15.523585056576408 21.778806332007353 2 14.609463018314027 20.959665066158674 41.30696700773717 23.123904907790127 3 18.35766818948315 26.004445842284547 30.71913542405197 24.918750544180334 4 11.854184362882906 17.497663210943635 38.86426720783029 31.783885218343173 5 12.632875222569329 38.55273960188077 34.826724667967426 13.987660507582461 6 29.799388229188327 38.27068992177794 18.095405675853893 13.83451617317984 7 27.441560380497375 33.7719859517411 21.413463862702443 17.37298980505908 8 25.764498683997733 35.80343671193146 20.057971632642545 18.374092971428265 9 26.250093297175493 17.03304319392662 20.9239796843179 35.79288382457999 10-11 24.02278075170438 27.776148634718872 27.95031883810702 20.250751775469727 12-13 25.154224717543492 25.260595784613255 28.492694431559062 21.09248506628419 14-15 22.332903285423864 26.474335735915044 26.93592641560399 24.256834563057108 16-17 20.96402394375209 30.033128825632893 27.56464031052942 21.438206920085594 18-19 21.001020070871824 28.151379228229306 30.51966746400429 20.327933236894577 20-21 21.775803655601177 27.290215211501877 30.505815002163576 20.42816613073337 22-23 21.624757773676873 27.00060506602861 30.383643848006702 20.990993312287817 24-25 21.21370160954997 27.47829470863556 30.48300714684256 20.824996534971906 26-27 20.977121537776696 27.588232105904293 30.390352821801397 21.04429353451761 28-29 20.93933776811754 27.536686494642538 30.640354166629642 20.883621570610284 30-31 21.05246788514719 27.520580519951377 30.546965528517706 20.87998606638373 32-33 20.868440929488603 27.68036610832186 30.35073532678722 21.10045763540232 34-35 21.312432570779574 27.586482696351222 30.209534913895 20.891549818974205 36-37 21.38066822806899 27.306740922127727 30.13974432333112 21.172846526472167 38-39 21.056461414893842 27.712187875384714 30.21611497700602 21.015235732715425 40-41 21.216517817478792 27.592481368678058 30.23205191537453 20.958948898468623 42-43 21.074517114137787 27.501948069833748 30.058828599175634 21.364706216852824 44-45 21.19362664510224 27.810914325830215 29.78192675796003 21.213532271107518 46-47 21.336911701976398 28.17911986350064 29.148034267026873 21.33593416749609 48-49 21.352453589447126 28.227143097094014 29.024936257419604 21.395467056039262 50-51 21.472004436489414 28.33674305509885 28.848823423810938 21.342429084600795 52-53 21.542113973757985 28.441442546481717 28.708893522691113 21.307549957069185 54-55 21.46812471950553 28.391239241231908 28.74769494643389 21.392941092828675 56-57 21.375402920574448 28.728814462849567 28.66129083846572 21.23449177811026 58-59 21.459866853576244 28.171466440669533 28.8677579201157 21.500908785638522 60-61 21.384148946848985 28.381111771431005 29.003648341457044 21.231090940262963 62-63 21.27817404722837 28.365538801585238 29.277733407839296 21.0785537433471 64-65 21.245139781526685 28.206456129005318 29.059331752716705 21.489072336751292 66-67 21.141147970446596 28.548864652910964 28.7416351378842 21.568352238758237 68-69 21.13233962904431 28.337273149641064 28.962224845805274 21.568162375509356 70-71 21.24864635811479 28.351457402588863 28.836236649026752 21.56365959026959 72-73 21.339377883909805 28.283415153308066 28.724052585102545 21.65315437767958 74-75 21.200655293789456 28.694867279908888 28.45348533495854 21.65099209134312 76-77 21.212438925352764 28.457164892440208 28.831498391334442 21.49889779087259 78-79 21.258914855412083 28.51127800843885 28.559522846996337 21.67028428915273 80-81 21.0945974639326 28.6975580156734 28.54608338123367 21.661761139160323 82-83 21.400447745282683 28.65187804271348 28.4567001883373 21.49097402366654 84-85 21.230091042834907 28.43054163734959 28.43063047696211 21.908736842853386 86-87 21.125311512986926 28.51411081644167 28.45440693702218 21.906170733549228 88-89 21.40048402228919 28.185591630181385 28.521955137317335 21.89196921021209 90-91 21.304890206259763 28.376488483963204 28.63158100498101 21.687040304796025 92-93 21.3616479150527 28.19485213114521 28.638618611361842 21.804881342440243 94-95 21.135485544578028 28.22109870623907 28.52874782455738 22.114667924625522 96-97 21.25796199565883 28.161808542491666 28.491616637183554 22.088612824665947 98-99 21.168341094611176 28.12585798327616 28.4319553177859 22.273845604326766 100-101 21.287987137006866 27.885068090360747 28.737863215215288 22.0890815574171 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 767.0 1 457.5 2 149.0 3 147.0 4 134.0 5 145.0 6 225.5 7 430.5 8 711.0 9 908.5 10 874.0 11 763.5 12 771.0 13 807.0 14 834.5 15 878.0 16 926.0 17 956.5 18 1039.5 19 1148.0 20 1259.0 21 1406.0 22 1625.5 23 1943.0 24 2265.5 25 2828.0 26 3783.5 27 4837.0 28 5597.5 29 6583.0 30 7922.5 31 9272.0 32 10735.5 33 12401.0 34 14217.0 35 15698.0 36 17571.5 37 19497.0 38 20129.0 39 20019.5 40 19812.0 41 20040.0 42 20089.5 43 20464.5 44 21193.0 45 21439.0 46 21363.5 47 20954.5 48 21217.0 49 20617.0 50 19007.5 51 17657.0 52 16312.5 53 15621.5 54 14951.5 55 13343.0 56 11615.0 57 10439.5 58 9427.0 59 8186.0 60 6761.0 61 5369.0 62 4126.5 63 3048.0 64 2244.0 65 1703.0 66 1231.5 67 894.0 68 660.5 69 478.0 70 356.0 71 246.0 72 199.0 73 158.0 74 112.5 75 78.0 76 50.0 77 38.5 78 24.0 79 15.0 80 16.0 81 12.5 82 6.0 83 2.5 84 3.0 85 3.0 86 2.0 87 2.5 88 1.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22593619873872978 2 0.20181742085139284 3 0.19561038242450465 4 0.20181742085139284 5 0.20039866921096128 6 0.2211479119522732 7 0.22061588008711136 8 0.2105072746490363 9 0.20536429995247182 10-11 0.21423149770516925 12-13 0.21689165703097846 14-15 0.20359086040193236 16-17 0.21706900098603238 18-19 0.20749242741311918 20-21 0.20403422028956722 22-23 0.19995530932332642 24-25 0.1968517901098823 26-27 0.20243812469408168 28-29 0.21334477792989948 30-31 0.21423149770516925 32-33 0.21299009001979158 34-35 0.2215025998623811 36-37 0.20110804503117707 38-39 0.1986252296604218 40-41 0.1975611659300981 42-43 0.20208343678397378 44-45 0.21653696912087056 46-47 0.21919712844667977 48-49 0.22371939930055543 50-51 0.22504947896346003 52-53 0.2381729316374522 54-55 0.22230064766012386 56-57 0.1968517901098823 58-59 0.1839056813909441 60-61 0.18044747426739213 62-63 0.18754123246955004 64-65 0.18009278635728423 66-67 0.18275294568309344 68-69 0.18310763359320134 70-71 0.18488107314374083 72-73 0.18709787258191515 74-75 0.19064475168299413 76-77 0.20261546864913565 78-79 0.1988912455930027 80-81 0.1907334236605211 82-83 0.1872752165369691 84-85 0.1886939681774007 86-87 0.19481233462676192 88-89 0.19365959891891124 90-91 0.20199476480644682 92-93 0.19153147145826388 94-95 0.18789592037965794 96-97 0.19942327745816457 98-99 0.20332484446935142 100-101 0.18106817811008094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 563876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.5445694614919 #Duplication Level Percentage of deduplicated Percentage of total 1 82.6435543966424 52.51549082902029 2 10.200309520852882 12.963485537531064 3 2.8477978148045864 5.428862581654047 4 1.2760927945769005 3.2435506889720473 5 0.6941336871618831 2.2054213149709887 6 0.4587085088501132 1.7489060821922038 7 0.3103809370877597 1.3806116111407267 8 0.23322181596721453 1.1855983907731158 9 0.18370941785604172 1.0506362277315138 >10 1.0683812157749333 12.425843046608035 >50 0.061945324544923054 2.660895698239709 >100 0.020369401400882413 1.8080326432858556 >500 5.580657918046812E-4 0.22855291528827001 >1k 8.37098687707022E-4 1.154112432592203 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.5004646411622414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2287 0.40558562520837915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.24828153707552725 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 755 0.13389468606573077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7734395505394804E-4 0.0 18-19 0.0 0.0 0.0 1.7734395505394804E-4 0.0 20-21 0.0 0.0 0.0 1.7734395505394804E-4 0.0 22-23 0.0 0.0 0.0 9.753917527967142E-4 0.0 24-25 0.0 0.0 0.0 0.0012414076853776363 0.0 26-27 0.0 0.0 0.0 0.0012414076853776363 0.0 28-29 0.0 0.0 0.0 0.0020394554831204025 0.0 30-31 0.0 0.0 0.0 0.004167582943767779 0.0 32-33 0.0 0.0 0.0 0.006384382381942129 0.0 34-35 0.0 0.0 0.0 0.007980477977427661 0.0 36-37 0.0 0.0 0.0 0.010197277415602012 0.0 38-39 0.0 0.0 0.0 0.011970716966141492 0.0 40-41 0.0 0.0 0.0 0.015074236179585582 0.0 42-43 0.0 0.0 0.0 0.023498074044648112 0.0 44-45 0.0 0.0 0.0 0.03573480694337053 0.0 46-47 0.0 0.0 0.0 0.05054302719037519 0.0 48-49 0.0 0.0 0.0 0.08352900283040951 0.0 50-51 0.0 0.0 0.0 0.11580560265022806 0.0 52-53 0.0 0.0 0.0 0.1491462662003703 0.0 54-55 0.0 0.0 0.0 0.1933935829863303 0.0 56-57 0.0 0.0 0.0 0.24934560080585094 0.0 58-59 0.0 0.0 0.0 0.3212585745802269 0.0 60-61 0.0 0.0 0.0 0.40265944994998903 0.0 62-63 0.0 0.0 0.0 0.5258248267349559 0.0 64-65 0.0 0.0 0.0 0.6294823684639885 0.0 66-67 0.0 0.0 0.0 0.7667465896757444 0.0 68-69 0.0 0.0 0.0 0.9549971979655101 0.0 70-71 0.0 0.0 0.0 1.0996211933120048 0.0 72-73 0.0 0.0 0.0 1.2882264895118785 0.0 74-75 0.0 0.0 0.0 1.5414736573289163 0.0 76-77 0.0 0.0 0.0 1.7490547567195625 0.0 78-79 0.0 0.0 0.0 1.9426256836609466 0.0 80-81 0.0 0.0 0.0 2.222119756825969 0.0 82-83 0.0 0.0 0.0 2.552688889046528 0.0 84-85 0.0 0.0 0.0 2.894164674502905 0.0 86-87 0.0 0.0 0.0 3.3285686924075506 0.0 88-89 0.0 0.0 0.0 3.834442324198937 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1825 0.0 42.950546 1 GTATCAA 2870 0.0 32.939636 1 ACCGTGC 75 3.075825E-4 31.667791 8 GGACCGA 85 6.401753E-4 27.94217 6 CAACGCA 3685 0.0 25.136578 5 TCAACGC 3690 0.0 25.102518 4 ATCAACG 3705 0.0 25.000889 3 TATCAAC 3730 0.0 24.958454 2 ATATAGG 270 1.8189894E-12 24.628317 2 AACGCAG 3815 0.0 24.529053 6 GTACATG 2395 0.0 24.000896 1 GGCCTAA 185 2.5359805E-7 23.108929 6 CATTCCG 125 2.2533738E-4 22.80081 9 TACATGG 2535 0.0 22.29664 2 TACCTGG 450 0.0 22.165485 2 ACATGGG 2450 0.0 22.102825 3 GAGTACT 2350 0.0 21.727564 12-13 CATGGGA 1540 0.0 21.591677 4 ACGCAGA 4245 0.0 21.484863 7 CGCAGAG 4225 0.0 21.361706 8 >>END_MODULE