FastQCFastQC Report
Thu 26 May 2016
SRR937272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563876
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36060.6395023019245366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.556150643049181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.3740184012087764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.13637750143648605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1152.4992914E-937.1707536
GGTATCA20500.036.4040151
GTATCAA33300.035.5433651
AGGACCG1503.3331162E-828.4975815
ATCAACG42050.028.011893
TATCAAC42500.027.8270472
TCAACGC42350.027.8134574
CAACGCA42400.027.780665
TATAGGG1554.58258E-827.5783022
AACGCAG43600.027.0160526
GTACATG25750.026.9512621
ACATGGG24950.026.079953
TACATGG27150.025.716052
GTATAGG2451.0913936E-1125.2220691
CATGGGG9600.024.2427334
ACGCAGA48900.023.8979157
TAACGCG500.001654443923.74798276-77
CGCAGAG48900.023.7036238
GTATTAC1452.3187882E-522.94741
GAGTACT29750.022.91388312-13
Produced by FastQC (version 0.11.5)