##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 563876 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.247877192858006 33.0 31.0 34.0 31.0 34.0 2 32.45854939738524 34.0 31.0 34.0 31.0 34.0 3 32.50879271329158 34.0 31.0 34.0 31.0 34.0 4 36.139594520781166 37.0 35.0 37.0 35.0 37.0 5 36.06679482723152 37.0 35.0 37.0 35.0 37.0 6 36.11559456334371 37.0 35.0 37.0 35.0 37.0 7 36.0595095375579 37.0 35.0 37.0 35.0 37.0 8 36.03592633841483 37.0 35.0 37.0 35.0 37.0 9 37.76144755229873 39.0 37.0 39.0 35.0 39.0 10-11 37.6761016606488 39.0 37.0 39.0 35.0 39.0 12-13 37.5889974036845 39.0 37.0 39.0 35.0 39.0 14-15 38.78786027424469 40.0 38.0 41.0 35.0 41.0 16-17 38.75584614347835 40.0 38.0 41.0 35.0 41.0 18-19 38.7827864636906 40.0 38.0 41.0 35.0 41.0 20-21 38.69602359383978 40.0 38.0 41.0 34.0 41.0 22-23 38.555753392589864 40.0 38.0 41.0 34.0 41.0 24-25 38.507779192588444 40.0 38.0 41.0 34.0 41.0 26-27 38.45749154069334 40.0 38.0 41.0 34.0 41.0 28-29 38.33012577233293 40.0 38.0 41.0 34.0 41.0 30-31 38.3743509211245 40.0 38.0 41.0 34.0 41.0 32-33 38.29068092984983 40.0 38.0 41.0 34.0 41.0 34-35 38.16478445615702 40.0 38.0 41.0 34.0 41.0 36-37 37.989674148216984 40.0 37.0 41.0 33.5 41.0 38-39 37.85873844604133 40.0 37.0 41.0 33.0 41.0 40-41 37.6748930615951 40.0 37.0 41.0 33.0 41.0 42-43 37.45311557860239 40.0 37.0 41.0 33.0 41.0 44-45 37.13592438763132 40.0 37.0 41.0 32.0 41.0 46-47 36.73409136051188 40.0 36.0 41.0 31.0 41.0 48-49 36.3725366924643 40.0 36.0 41.0 30.0 41.0 50-51 36.12848037511793 39.0 36.0 41.0 29.5 41.0 52-53 35.89985830217991 39.0 35.0 41.0 29.0 41.0 54-55 35.6765175322234 39.0 35.0 40.0 28.0 41.0 56-57 35.426743645766095 39.0 35.0 40.0 28.0 41.0 58-59 35.17108814703942 38.0 35.0 40.0 27.0 41.0 60-61 34.875100199334604 38.0 34.0 40.0 26.5 41.0 62-63 34.52326486674375 38.0 34.0 40.0 26.0 41.0 64-65 34.137567479374894 37.0 34.0 40.0 26.0 41.0 66-67 33.7281352637814 36.5 33.5 39.0 25.0 41.0 68-69 33.30205311096766 36.0 33.0 39.0 23.0 40.0 70-71 32.90629234086927 36.0 32.5 39.0 22.5 40.0 72-73 32.44551284324922 35.0 32.0 38.0 22.0 40.0 74-75 31.966047499804922 35.0 31.5 37.0 20.5 39.0 76-77 30.6931293404933 34.0 30.0 36.0 20.0 38.5 78-79 31.287443515950315 34.5 31.0 36.0 20.0 39.0 80-81 31.089504607395952 35.0 31.0 36.0 18.5 37.5 82-83 30.737799622612066 34.0 31.0 35.5 17.5 37.0 84-85 30.36713479559336 34.0 31.0 35.0 13.0 37.0 86-87 29.954309990139677 34.0 30.0 35.0 7.5 36.0 88-89 29.497290184366776 34.0 30.0 35.0 4.5 36.0 90-91 28.9964611013769 34.0 29.0 35.0 2.0 35.5 92-93 28.647762096631176 34.0 29.0 35.0 2.0 35.0 94-95 28.229925551007668 34.0 29.0 35.0 2.0 35.0 96-97 27.776640608928204 33.5 28.5 35.0 2.0 35.0 98-99 27.047800402925468 33.0 26.5 35.0 2.0 35.0 100-101 24.545460349438528 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07299266014846495 1101 2 -0.06608751933726609 1101 3 -0.024689204975175016 1101 4 -0.012015306979215268 1101 5 -0.06478655143676804 1101 6 -0.14018100424588198 1101 7 -0.1173141170694123 1101 8 -0.12548823277123233 1101 9 -0.09585396699956306 1101 10-11 -0.18974093054386287 1101 12-13 -0.13818812107427192 1101 14-15 -0.21978347825362476 1101 16-17 -0.18076314266001248 1101 18-19 -0.228979275134769 1101 20-21 -0.3657882064767719 1101 22-23 -0.1951691688082846 1101 24-25 -0.1086782713165988 1101 26-27 -0.06998882772001735 1101 28-29 -0.28037924976248974 1101 30-31 -0.2386808327366694 1101 32-33 -0.13745233305001392 1101 34-35 -0.11909677358753612 1101 36-37 -0.15643421722462136 1101 38-39 -0.21412775352004587 1101 40-41 0.005110432687715161 1101 42-43 0.28381272836966076 1101 44-45 0.36326304530012266 1101 46-47 0.22397391009985057 1101 48-49 0.23625811177672063 1101 50-51 0.2948530470014816 1101 52-53 0.24168538174513543 1101 54-55 0.2935852262265257 1101 56-57 0.4904435501288873 1101 58-59 0.34745451257222015 1101 60-61 0.1710431416312801 1101 62-63 0.13615392325539233 1101 64-65 0.20830673524823595 1101 66-67 0.2973829562190815 1101 68-69 0.12269782637317661 1101 70-71 -0.09247397707683547 1101 72-73 0.21303985782371626 1101 74-75 0.019366607109230216 1101 76-77 0.16623605357052185 1101 78-79 -0.1127953271678912 1101 80-81 -0.03130053258229637 1101 82-83 -0.03851966947982888 1101 84-85 0.0037497567371502782 1101 86-87 -0.09803122891594285 1101 88-89 0.048101434900015505 1101 90-91 -0.26187795668698044 1101 92-93 -0.4223337028617635 1101 94-95 -0.456695437962793 1101 96-97 -0.2589359235139419 1101 98-99 -0.1912333170038032 1101 100-101 0.18506587517080675 1102 1 -0.007264582195446678 1102 2 0.005472406745887781 1102 3 -0.033822831468214076 1102 4 -0.10741908820206447 1102 5 0.07195513664966313 1102 6 -0.11818059226991551 1102 7 -0.09283289332169176 1102 8 -0.04114063253121003 1102 9 -0.06250182606796528 1102 10-11 -0.0798702253186292 1102 12-13 -0.36648997407049677 1102 14-15 -0.10574002374562497 1102 16-17 0.0407224486299782 1102 18-19 -0.08441228276826251 1102 20-21 0.048270771763135656 1102 22-23 -0.0662303950039771 1102 24-25 -0.11349361354025689 1102 26-27 -0.1162823874610055 1102 28-29 -0.16696196886354642 1102 30-31 -0.012385869889143919 1102 32-33 -0.10653995556061346 1102 34-35 -0.08013146017233908 1102 36-37 -0.03051660047571403 1102 38-39 0.005451702611978249 1102 40-41 0.04271741956119968 1102 42-43 0.16571593790123273 1102 44-45 0.22595862193212923 1102 46-47 0.10196558699128389 1102 48-49 0.10854929519259571 1102 50-51 0.12099098133013086 1102 52-53 -0.07347772192651547 1102 54-55 0.07752323877090106 1102 56-57 -0.006985331774330916 1102 58-59 0.009212545357151214 1102 60-61 -0.07140769739658026 1102 62-63 0.060284668902028216 1102 64-65 0.0320937197931741 1102 66-67 0.1599994215250362 1102 68-69 0.13442048296136733 1102 70-71 -0.003703605910374108 1102 72-73 0.07488834620031781 1102 74-75 0.17506665278482458 1102 76-77 0.23357322604217146 1102 78-79 0.07079146094212518 1102 80-81 -0.09548593967723917 1102 82-83 0.11809836611131175 1102 84-85 -0.010163387982842664 1102 86-87 -0.03013729182885072 1102 88-89 0.19393719381646335 1102 90-91 -0.0015240753062037982 1102 92-93 -8.13630974938917E-4 1102 94-95 0.05512648094958905 1102 96-97 0.3105581520926002 1102 98-99 0.34059481140134196 1102 100-101 0.8226613685120228 1103 1 -0.03177315250554358 1103 2 -0.05774469845594865 1103 3 -0.03057459539554941 1103 4 -0.10954418956381318 1103 5 -0.036448334349231004 1103 6 -0.2109021201059491 1103 7 -0.14225548091221185 1103 8 -0.18220821634688633 1103 9 -0.22319863561973108 1103 10-11 -0.039602816730614165 1103 12-13 -0.16638791328141878 1103 14-15 -0.19732909625072637 1103 16-17 -0.25162112806474823 1103 18-19 -0.12377792137053945 1103 20-21 -0.1484315928781612 1103 22-23 -0.05550935184019323 1103 24-25 -0.03526797997111686 1103 26-27 -0.2127857568651379 1103 28-29 -0.3191071095583027 1103 30-31 -0.35030727842124065 1103 32-33 -0.12174188679308173 1103 34-35 -0.4755890560116143 1103 36-37 -0.2411020286183927 1103 38-39 -0.22524529335218801 1103 40-41 -0.1934950532924944 1103 42-43 -0.3318607008967831 1103 44-45 -0.28689684321341247 1103 46-47 -0.03417604915035355 1103 48-49 0.05876786373178078 1103 50-51 0.0016940356973051962 1103 52-53 0.09893044666485906 1103 54-55 0.10154014637559783 1103 56-57 -0.15635525786180438 1103 58-59 -0.14917974089773622 1103 60-61 -0.0851253198928319 1103 62-63 0.00814046026926718 1103 64-65 -0.28063638301326677 1103 66-67 0.04807974662825387 1103 68-69 0.2051057349443255 1103 70-71 0.16580351970362273 1103 72-73 0.10448364487958628 1103 74-75 -0.011278172872977166 1103 76-77 0.21169074041922897 1103 78-79 0.09910983468034473 1103 80-81 0.11522764699054378 1103 82-83 0.08979397330310235 1103 84-85 0.021734103838312535 1103 86-87 -0.1576550243084185 1103 88-89 -0.03276824662943767 1103 90-91 -0.011692871734542365 1103 92-93 0.14548049051001755 1103 94-95 0.18792434466348595 1103 96-97 0.3195618710199817 1103 98-99 0.1940700045711914 1103 100-101 0.82552046869937 1104 1 0.21068413575326872 1104 2 0.1342237723393609 1104 3 0.11015891777669395 1104 4 0.016448272507396666 1104 5 0.05322240739061357 1104 6 0.12377043915479646 1104 7 0.16338457342209267 1104 8 0.06672518586091769 1104 9 0.05914845347705011 1104 10-11 0.11391552109868996 1104 12-13 0.10565191912402128 1104 14-15 0.1417055131735978 1104 16-17 0.011675472214584204 1104 18-19 0.07802907704152062 1104 20-21 0.00791346824793493 1104 22-23 0.0642078801711321 1104 24-25 0.11422311417647535 1104 26-27 -0.01950197489112071 1104 28-29 0.15599285409128072 1104 30-31 0.010427573976933502 1104 32-33 0.10710312650351739 1104 34-35 0.22331947485754 1104 36-37 -0.0173747715444037 1104 38-39 0.05941674341912773 1104 40-41 0.12979408558523176 1104 42-43 0.18472178585444965 1104 44-45 0.340138301901284 1104 46-47 0.36494675786718744 1104 48-49 0.5255694095811307 1104 50-51 0.5120969513834694 1104 52-53 0.5360107428251055 1104 54-55 0.5355593547017463 1104 56-57 0.4621040583677001 1104 58-59 0.5114938897437611 1104 60-61 0.5599463286942878 1104 62-63 0.7160265878018421 1104 64-65 0.5934811627595593 1104 66-67 0.4920474904152101 1104 68-69 0.6201701134478341 1104 70-71 0.757076549606623 1104 72-73 0.6772275274868704 1104 74-75 0.418029829958531 1104 76-77 0.4150043679996287 1104 78-79 0.2949094482180108 1104 80-81 0.3751618336021103 1104 82-83 0.3751351889376089 1104 84-85 0.27993686264372286 1104 86-87 0.517128308068326 1104 88-89 0.5076381127805369 1104 90-91 0.5061714455472135 1104 92-93 0.6885700847245637 1104 94-95 0.8169348480211163 1104 96-97 1.0382909456148681 1104 98-99 0.7607156262589996 1104 100-101 1.4290330669889855 1105 1 0.00996763077578322 1105 2 0.023515910808036722 1105 3 0.03814632971533172 1105 4 -0.11442318042586663 1105 5 -0.050707523962401524 1105 6 -0.011324288343040223 1105 7 -0.01792422453818432 1105 8 -0.09407127933669557 1105 9 -0.02412564388352223 1105 10-11 -0.09245092764633966 1105 12-13 -0.019536217662682986 1105 14-15 -0.09249516829038384 1105 16-17 -0.04571500798058281 1105 18-19 -0.13154195439859961 1105 20-21 -0.20221738485018648 1105 22-23 -0.04371422202176234 1105 24-25 -0.01449415169125956 1105 26-27 -0.24939428222403137 1105 28-29 -0.20054763161032696 1105 30-31 0.03364473805891066 1105 32-33 -0.1764069135767059 1105 34-35 -0.16256367509456737 1105 36-37 -0.31153060053098613 1105 38-39 -0.28667568472179994 1105 40-41 -0.3139417520114165 1105 42-43 -0.40683283304966267 1105 44-45 -0.4738799327666072 1105 46-47 -0.7274738742724054 1105 48-49 -0.728767331072099 1105 50-51 -0.6094969801831311 1105 52-53 -0.38022542015225014 1105 54-55 -0.5846179193755816 1105 56-57 -0.46705972993127887 1105 58-59 -0.2739150387025262 1105 60-61 -0.5000555401589324 1105 62-63 -0.38358829494430324 1105 64-65 -0.4263979291112676 1105 66-67 -0.37019800347930953 1105 68-69 -0.2418259219135379 1105 70-71 -0.11590483861759537 1105 72-73 -0.3205315172621539 1105 74-75 -0.12740916700124316 1105 76-77 -0.3770298119909583 1105 78-79 -0.39436366799462874 1105 80-81 -0.5086030045919081 1105 82-83 -0.7710116361811146 1105 84-85 -0.6567615745277635 1105 86-87 -0.6912988850809896 1105 88-89 -0.574329455941534 1105 90-91 -0.38358039744242234 1105 92-93 -0.010976718368389982 1105 94-95 0.025056683902427324 1105 96-97 0.3564456866618926 1105 98-99 0.3140864034015891 1105 100-101 0.7299693290823015 1106 1 -0.11413083422299053 1106 2 -0.1359274120732863 1106 3 0.006600902503912209 1106 4 -0.03480890268865977 1106 5 -0.10120324618614518 1106 6 -0.1614330387481715 1106 7 -0.1331742723727416 1106 8 -0.11518486786577142 1106 9 -0.05024526542430863 1106 10-11 -0.1623782191169454 1106 12-13 -0.10347610621791858 1106 14-15 -0.33212385496816665 1106 16-17 -0.17555947732936517 1106 18-19 -0.1035392131855204 1106 20-21 -0.25954954412169684 1106 22-23 -0.4051638629697081 1106 24-25 -0.34569868445500873 1106 26-27 -0.4059685077922879 1106 28-29 -0.38869364220812486 1106 30-31 -0.3444552342165679 1106 32-33 -0.19484860032087425 1106 34-35 -0.36266344431722786 1106 36-37 -0.4214017010382278 1106 38-39 -0.5948288412169518 1106 40-41 -0.2856764088326287 1106 42-43 -0.45212142187160964 1106 44-45 -0.29800397461110606 1106 46-47 -0.25216236391086255 1106 48-49 -0.43717469407977916 1106 50-51 -0.09964501349941202 1106 52-53 -0.1122337687470818 1106 54-55 -0.5438804471489149 1106 56-57 -0.3642212857844669 1106 58-59 -0.6008691292406496 1106 60-61 -0.6176016468163326 1106 62-63 -0.30648386722134546 1106 64-65 -0.17173650016608377 1106 66-67 -0.3157730139248187 1106 68-69 -0.27058132526625656 1106 70-71 -0.2671059741514483 1106 72-73 -0.34140593299718347 1106 74-75 -0.49455404909393863 1106 76-77 -0.30851998540588 1106 78-79 -0.23028917884819222 1106 80-81 -0.3498820811701542 1106 82-83 -0.3709878165232574 1106 84-85 -0.03554392142789098 1106 86-87 0.20871614863749244 1106 88-89 0.14081379391886983 1106 90-91 -0.27471772753211354 1106 92-93 -0.12701359588458416 1106 94-95 -0.3031362240873108 1106 96-97 -0.4994463595248142 1106 98-99 -0.44437738486117695 1106 100-101 0.18809163716809962 1107 1 -0.03563621056707689 1107 2 -0.02668987541639467 1107 3 -0.02937172701710722 1107 4 -0.012863643646625178 1107 5 0.07968629438082075 1107 6 -0.11275268684201478 1107 7 -0.07476762525642044 1107 8 0.03852969713911847 1107 9 -0.018329331895465373 1107 10-11 0.02239695194854363 1107 12-13 -0.06210269468321883 1107 14-15 -0.025012418018015126 1107 16-17 0.029572487480017173 1107 18-19 -0.01649131484729338 1107 20-21 0.03412103261339183 1107 22-23 0.006444152670560754 1107 24-25 -0.0342617343083802 1107 26-27 -0.12305061922923954 1107 28-29 0.0023132004116206417 1107 30-31 -0.0121971697004426 1107 32-33 0.02523937621871397 1107 34-35 -0.021656601697479516 1107 36-37 -0.043542463501573536 1107 38-39 -0.08688651472623832 1107 40-41 -0.10461580277201676 1107 42-43 -0.1624586652733626 1107 44-45 0.05487970085320626 1107 46-47 0.20597546600116345 1107 48-49 0.14728278392608019 1107 50-51 0.12530611064525488 1107 52-53 0.04810347465467402 1107 54-55 0.24715083339849286 1107 56-57 0.06285593806694578 1107 58-59 -0.06665603051142455 1107 60-61 0.06819991721103236 1107 62-63 0.19165885027620533 1107 64-65 0.0474228351222834 1107 66-67 0.2916316781572874 1107 68-69 0.41067478421566506 1107 70-71 0.33773273552596805 1107 72-73 0.32755789886987685 1107 74-75 0.21724946996764416 1107 76-77 0.19038030784925297 1107 78-79 0.21675383190449793 1107 80-81 0.3213056021143039 1107 82-83 0.3990757220886678 1107 84-85 0.4922175643981106 1107 86-87 0.4858911241995614 1107 88-89 0.4701637450430134 1107 90-91 0.5300721062899783 1107 92-93 0.6844233542620444 1107 94-95 0.4565167573398661 1107 96-97 0.6711059376403874 1107 98-99 1.0828220286285593 1107 100-101 2.0227446185952758 1108 1 -0.14965313488040977 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138.0 9 243.0 10 462.0 11 715.0 12 1149.0 13 1664.0 14 2171.0 15 3139.0 16 4525.0 17 6878.0 18 5892.0 19 3344.0 20 2624.0 21 2653.0 22 2699.0 23 2920.0 24 3476.0 25 3924.0 26 4893.0 27 5938.0 28 7450.0 29 9417.0 30 11926.0 31 14933.0 32 19075.0 33 25516.0 34 35121.0 35 51761.0 36 79571.0 37 128347.0 38 110877.0 39 10347.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.592130388795425 20.806979435086 16.251925520511673 21.348964655606903 2 15.218771502954551 21.38892238719151 40.40693343926679 22.98537267058715 3 18.385425164397844 25.860650213876813 31.333839354751753 24.42008526697359 4 12.33533613773241 18.15328192723223 38.646439997446244 30.864941937589112 5 12.6498812654847 38.36180065015793 35.005488921954445 13.982829162402924 6 30.102717618767244 38.37971468904511 17.645014152047615 13.872553540140032 7 27.162709531882896 34.09579411076194 21.421021643056275 17.32047471429889 8 26.09882314551426 35.700579560045114 19.84993154523335 18.350665749207273 9 26.25985145670325 16.79589129524931 20.915414027197468 36.02884322084998 10-11 24.090512888040003 27.780334488730222 27.792659904535665 20.33649271869411 12-13 25.347546446677327 25.2464549242656 28.542215908435907 20.863782720621163 14-15 22.446335719200675 26.333449198050634 27.252002213252556 23.96821286949613 16-17 20.89368939270336 29.859579056388284 27.89283459484 21.353896956068354 18-19 20.802445927828103 27.70759883378615 31.300764707134192 20.18919053125155 20-21 21.370301271910847 26.823539217842217 31.624506097085174 20.18165341316176 22-23 21.35043179164047 26.494511604150944 31.592481620369178 20.5625749838394 24-25 21.06813058968952 26.665578951288676 31.712460075229703 20.553830383792096 26-27 20.891775659499 26.955530924407007 31.610463311904237 20.542230104189755 28-29 20.708952576880783 26.843986095839394 31.864558578370517 20.582502748909302 30-31 20.80372422966083 26.956861006428728 31.59361560629572 20.645799157614718 32-33 20.78409082848002 27.036351963906956 31.41444218232377 20.765115025289248 34-35 21.16307486043031 26.832849775482554 31.336588186055092 20.667487178032047 36-37 21.25520504508083 26.702679312472956 31.305996353808286 20.736119288637926 38-39 20.913023931786118 27.074778296905244 31.359338575052735 20.6528591962559 40-41 21.040656173797384 26.92547107071603 31.446685878962537 20.58718687652405 42-43 21.052645581474543 26.973012089221864 31.07582723196549 20.898515097338098 44-45 21.134471292396366 27.074351585014412 30.81800044336067 20.97317667922855 46-47 21.15917329341912 27.59046315147302 30.194847803417773 21.055515751690088 48-49 21.24979605445169 27.739343401740808 29.84042590924246 21.170434634565048 50-51 21.553763593414153 27.7278160446623 29.639051848278697 21.079368513644845 52-53 21.598631614042805 27.957476466457166 29.36505543772035 21.078836481779682 54-55 21.473781469684823 27.903563904120766 29.439628570820535 21.183026055373876 56-57 21.40009505635991 28.268892451531897 29.33251282196795 20.998499670140244 58-59 21.45844121757266 27.707776177741206 29.487156750774997 21.346625853911146 60-61 21.383956756449997 28.02823670452369 29.618923309380072 20.968883229646234 62-63 21.308851591484654 27.9165100128397 29.85443608169172 20.92020231398393 64-65 21.18437491964095 27.737949245888498 29.880886826968013 21.19678900750254 66-67 21.161390092857296 28.00509331838915 29.51340365612298 21.32011293263058 68-69 21.21069171236229 27.761068036234914 29.613869006661037 21.41437124474175 70-71 21.227007356227258 27.902233824457863 29.608903375919525 21.261855443395355 72-73 21.269392561485148 27.844774383020383 29.32843391100171 21.557399144492763 74-75 21.15181351928438 28.195826742049668 29.02748121927516 21.62487851939079 76-77 21.165291659868483 27.97512218998503 29.448141080663127 21.411445069483364 78-79 21.26531365051891 27.991526505827522 29.18567202718328 21.55748781647029 80-81 21.222396413395852 28.236261163801974 28.98083975909597 21.560502663706206 82-83 21.30545006025277 28.207695152024076 28.994306357176995 21.49254843054616 84-85 21.284630963655 28.073825003458218 28.882250289958396 21.759293742928385 86-87 21.023327823847797 27.956589746681892 28.987135469500387 22.032946959969923 88-89 21.23747064957544 27.774102816931386 29.035727713185167 21.95269882030801 90-91 21.11404546494531 27.85166870928436 29.22365156193743 21.810634263832895 92-93 21.022697042893473 27.65029386622968 29.136650457026718 22.190358633850128 94-95 20.909739020635744 27.621675687562515 29.205268534216742 22.263316757585 96-97 21.161478764834822 27.575832275181067 29.062683994353367 22.20000496563074 98-99 20.93130487137675 27.55351136908768 28.89724770804903 22.61793605148654 100-101 20.585838251530888 27.571821942773024 29.19888437099542 22.643455434700673 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 133.0 2 90.0 3 122.0 4 122.5 5 131.0 6 217.0 7 475.5 8 846.0 9 1046.0 10 1002.0 11 913.0 12 916.0 13 982.0 14 1048.0 15 1104.0 16 1152.0 17 1187.0 18 1239.0 19 1311.0 20 1419.5 21 1555.0 22 1764.0 23 2105.5 24 2505.0 25 3004.0 26 3760.5 27 4841.0 28 5789.5 29 6697.0 30 7864.0 31 9300.5 32 10831.5 33 12205.5 34 13885.0 35 15583.0 36 17698.0 37 19345.5 38 20004.0 39 20124.0 40 19934.5 41 20097.5 42 19900.0 43 20179.5 44 21073.5 45 21381.5 46 20888.5 47 20815.0 48 21422.0 49 20534.5 50 18999.5 51 17753.0 52 16398.5 53 15771.5 54 14934.5 55 13221.0 56 11537.0 57 10365.0 58 9331.0 59 8072.5 60 6574.5 61 5198.5 62 4020.0 63 2976.0 64 2199.5 65 1601.0 66 1152.0 67 871.0 68 633.5 69 424.5 70 314.5 71 231.0 72 160.5 73 125.5 74 102.0 75 95.5 76 73.0 77 34.0 78 17.5 79 13.0 80 9.5 81 7.5 82 4.0 83 2.5 84 2.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06951883038114763 2 0.0 3 0.0 4 0.0 5 0.0023054714157013244 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.867197752697402E-5 12-13 0.005408990629145415 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.980477977427662E-4 24-25 5.320318651618442E-4 26-27 1.7734395505394804E-4 28-29 0.0028375032808631686 30-31 1.7734395505394804E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.4335988763487006E-4 40-41 1.7734395505394804E-4 42-43 0.012059388943668467 44-45 1.7734395505394804E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.867197752697402E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.660159325809221E-4 84-85 3.5468791010789607E-4 86-87 0.0 88-89 0.0 90-91 0.010108605438075038 92-93 0.0024828153707552726 94-95 0.0 96-97 0.0 98-99 8.867197752697402E-5 100-101 0.01214806092119544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 563876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.39376685382957 #Duplication Level Percentage of deduplicated Percentage of total 1 81.64081791576263 50.12237340876282 2 10.592006426170283 13.005663460851261 3 3.1091766060247066 5.726521909729859 4 1.3878179515788822 3.4081348701917293 5 0.7862144494682055 2.4134333303881492 6 0.47254438518452735 1.7406767887263062 7 0.3310293749045213 1.4226198185259984 8 0.2602806538333839 1.2783687822407275 9 0.18831602210878073 1.040528696056839 >10 1.1323818799699576 12.762245558790195 >50 0.0730391073136201 3.0291116212653098 >100 0.02521587701323091 2.3385171995983702 >500 2.898376668187073E-4 0.13683759903294626 >1k 8.695130004561218E-4 1.5749669558395414 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3606 0.6395023019245366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3136 0.556150643049181 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2109 0.3740184012087764 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 769 0.13637750143648605 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7734395505394804E-4 0.0 18-19 0.0 0.0 0.0 1.7734395505394804E-4 0.0 20-21 0.0 0.0 0.0 1.7734395505394804E-4 0.0 22-23 0.0 0.0 0.0 9.753917527967142E-4 0.0 24-25 0.0 0.0 0.0 0.0012414076853776363 0.0 26-27 0.0 0.0 0.0 0.0012414076853776363 0.0 28-29 0.0 0.0 0.0 0.0021281274606473766 0.0 30-31 0.0 0.0 0.0 0.004256254921294752 0.0 32-33 0.0 0.0 0.0 0.006561726336996077 0.0 34-35 0.0 0.0 0.0 0.008157821932481608 0.0 36-37 1.7734395505394804E-4 0.0 0.0 0.01037462137065596 0.0 38-39 1.7734395505394804E-4 0.0 0.0 0.012059388943668467 0.0 40-41 1.7734395505394804E-4 0.0 0.0 0.01525158013463953 0.0 42-43 1.7734395505394804E-4 0.0 0.0 0.023320730089594167 0.0 44-45 1.7734395505394804E-4 0.0 0.0 0.035291447055735656 0.0 46-47 1.7734395505394804E-4 0.0 0.0 0.05009966730274032 0.0 48-49 1.7734395505394804E-4 0.0 0.0 0.08344033085288255 0.0 50-51 1.7734395505394804E-4 0.0 0.0 0.11616029056033596 0.0 52-53 1.7734395505394804E-4 0.0 0.0 0.150210329930694 0.0 54-55 1.7734395505394804E-4 0.0 0.0 0.19365959891891124 0.0 56-57 1.7734395505394804E-4 0.0 0.0 0.2494342727833779 0.0 58-59 1.7734395505394804E-4 0.0 0.0 0.3213472465577538 0.0 60-61 1.7734395505394804E-4 0.0 0.0 0.402570777972462 0.0 62-63 1.7734395505394804E-4 0.0 0.0 0.5258248267349559 0.0 64-65 1.7734395505394804E-4 0.0 0.0 0.6289503365988267 0.0 66-67 1.7734395505394804E-4 0.0 0.0 0.7671899495633792 0.0 68-69 1.7734395505394804E-4 0.0 0.0 0.9546425100554022 0.0 70-71 1.7734395505394804E-4 0.0 0.0 1.1003305691322205 0.0 72-73 1.7734395505394804E-4 0.0 0.0 1.289911257084891 0.0 74-75 1.7734395505394804E-4 0.0 0.0 1.5454638963176301 0.0 76-77 1.7734395505394804E-4 0.0 0.0 1.754552419326235 0.0 78-79 1.7734395505394804E-4 0.0 0.0 1.948744050110308 0.0 80-81 1.7734395505394804E-4 0.0 0.0 2.227794763387695 0.0 82-83 1.7734395505394804E-4 0.0 0.0 2.560314679113848 0.0 84-85 1.7734395505394804E-4 0.0 0.0 2.9053373436713033 0.0 86-87 1.7734395505394804E-4 0.0 0.0 3.3431108967219743 0.0 88-89 1.7734395505394804E-4 0.0 0.0 3.8541275032099254 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 115 2.4992914E-9 37.170753 6 GGTATCA 2050 0.0 36.404015 1 GTATCAA 3330 0.0 35.543365 1 AGGACCG 150 3.3331162E-8 28.497581 5 ATCAACG 4205 0.0 28.01189 3 TATCAAC 4250 0.0 27.827047 2 TCAACGC 4235 0.0 27.813457 4 CAACGCA 4240 0.0 27.78066 5 TATAGGG 155 4.58258E-8 27.578302 2 AACGCAG 4360 0.0 27.016052 6 GTACATG 2575 0.0 26.951262 1 ACATGGG 2495 0.0 26.07995 3 TACATGG 2715 0.0 25.71605 2 GTATAGG 245 1.0913936E-11 25.222069 1 CATGGGG 960 0.0 24.242733 4 ACGCAGA 4890 0.0 23.897915 7 TAACGCG 50 0.0016544439 23.747982 76-77 CGCAGAG 4890 0.0 23.703623 8 GTATTAC 145 2.3187882E-5 22.9474 1 GAGTACT 2975 0.0 22.913883 12-13 >>END_MODULE