FastQCFastQC Report
Thu 26 May 2016
SRR937271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633600
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76311.2043876262626263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.8832070707070707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43470.6860795454545454No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27200.4292929292929293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18610.2937184343434343No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15540.24526515151515155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.21038510101010102No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12500.19728535353535354No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10870.17155934343434343No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9900.15625No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9730.15356691919191917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.15151515151515152No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8650.13652146464646464No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8540.13478535353535354No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.11963383838383837No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7200.11363636363636363No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6930.10937500000000001No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6860.10827020202020202No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6500.10258838383838385No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6370.10053661616161616No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6350.10022095959595959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1500.038.018821
GTACATG76050.026.8081421
TACATGG78200.025.8785442
ATGGGAG19000.025.2545195
ACATGGG78550.024.9145873
CATGGGG15050.024.3066734
GAGTACT50700.023.9847212-13
GTATCAA111350.022.9615231
GTATATA4350.022.942391
AGTACTT52450.022.8674912-13
GATAGTC1252.2528225E-422.8022777
CATGGGA61600.022.5202624
GTACTTT54800.022.44851114-15
TACCTGG4700.022.2362632
CGTCACT1100.002867699821.6016031
TGGGAGA9900.021.5913586
ATGGGAT22200.021.1862225
CATGGGT4950.021.1148874
CCTAGAC1150.003721006720.6526033
ATGGGGG8100.020.5283625
Produced by FastQC (version 0.11.5)