##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937271_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 633600 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.761089015151516 33.0 31.0 34.0 30.0 34.0 2 31.81290404040404 33.0 31.0 34.0 30.0 34.0 3 31.82602746212121 33.0 31.0 34.0 30.0 34.0 4 35.49438446969697 37.0 35.0 37.0 33.0 37.0 5 35.531246843434346 37.0 35.0 37.0 33.0 37.0 6 35.61637310606061 37.0 35.0 37.0 33.0 37.0 7 35.559943181818184 37.0 35.0 37.0 33.0 37.0 8 35.552488952020205 37.0 35.0 37.0 33.0 37.0 9 37.11866950757576 39.0 37.0 39.0 33.0 39.0 10-11 37.00278409090909 39.0 37.0 39.0 33.0 39.0 12-13 36.85466145833333 39.0 37.0 39.0 33.0 39.0 14-15 37.83443734217172 40.0 37.5 41.0 33.0 41.0 16-17 37.81774305555555 40.0 37.5 41.0 33.0 41.0 18-19 37.8639117739899 40.0 37.5 41.0 33.0 41.0 20-21 37.79887784090909 40.0 37.5 41.0 33.0 41.0 22-23 37.846796085858585 40.0 37.5 41.0 33.0 41.0 24-25 37.792612847222216 40.0 37.0 41.0 33.0 41.0 26-27 37.68231928661616 40.0 37.0 41.0 33.0 41.0 28-29 37.541687973484855 40.0 37.0 41.0 32.5 41.0 30-31 37.19136600378788 39.0 36.5 41.0 32.0 41.0 32-33 36.84157196969697 39.0 35.0 40.5 31.0 41.0 34-35 36.75334201388888 39.0 35.0 41.0 31.0 41.0 36-37 36.55867108585859 39.0 35.0 41.0 30.5 41.0 38-39 36.36997711489899 39.0 35.0 41.0 30.0 41.0 40-41 36.07572048611111 39.0 35.0 40.5 30.0 41.0 42-43 35.69005839646465 38.0 35.0 40.0 29.0 41.0 44-45 35.246109532828285 38.0 35.0 40.0 26.5 41.0 46-47 34.59287484217172 38.0 34.5 40.0 23.5 41.0 48-49 34.06404829545455 38.0 34.0 40.0 21.0 41.0 50-51 33.031586963383845 37.5 32.0 39.5 16.5 40.5 52-53 33.11058554292929 37.5 33.0 39.5 16.5 40.0 54-55 33.40506155303031 38.0 33.0 40.0 14.5 41.0 56-57 33.13960779671717 38.0 33.0 40.0 10.5 41.0 58-59 32.86557607323232 37.0 33.0 40.0 8.0 41.0 60-61 32.50112215909091 37.0 32.0 40.0 7.0 41.0 62-63 32.124191919191915 36.0 31.5 39.0 2.0 41.0 64-65 31.680044191919194 36.0 31.0 39.0 2.0 40.5 66-67 31.22572048611111 35.0 31.0 39.0 2.0 40.0 68-69 30.74119870580808 35.0 30.0 38.0 2.0 40.0 70-71 30.24199889520202 35.0 29.5 37.5 2.0 40.0 72-73 29.737495265151516 34.5 29.0 37.0 2.0 39.0 74-75 29.185754419191916 34.0 28.5 36.5 2.0 39.0 76-77 28.644116950757578 34.0 27.5 36.0 2.0 39.0 78-79 28.10616319444444 34.0 26.5 35.5 2.0 37.5 80-81 27.51175505050505 33.5 26.0 35.0 2.0 37.0 82-83 26.946654829545455 33.0 25.0 35.0 2.0 36.5 84-85 26.36447206439394 33.0 24.0 35.0 2.0 36.0 86-87 25.765144412878787 32.0 21.0 35.0 2.0 35.5 88-89 25.15621369949495 32.0 19.0 35.0 2.0 35.0 90-91 24.55469696969697 31.5 15.0 34.0 2.0 35.0 92-93 23.865328282828283 31.0 4.5 34.0 2.0 35.0 94-95 23.068050031565654 31.0 2.0 34.0 2.0 35.0 96-97 22.170008680555554 30.0 2.0 34.0 2.0 35.0 98-99 21.0079853219697 29.5 2.0 34.0 2.0 35.0 100-101 18.12449731691919 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41985312548043296 1101 2 0.36894549824643263 1101 3 0.2637224986452722 1101 4 0.32044885779258436 1101 5 0.3652470915618622 1101 6 0.2802678698722829 1101 7 0.4322622298219869 1101 8 0.4399968399314531 1101 9 0.44598791036222707 1101 10-11 0.55755389689363 1101 12-13 0.7080036516648036 1101 14-15 1.0173303801947355 1101 16-17 1.0099908919227332 1101 18-19 0.756608819031193 1101 20-21 0.7309434449040353 1101 22-23 0.7621301146320363 1101 24-25 0.8735283611956035 1101 26-27 0.8656473429672573 1101 28-29 0.745337312455284 1101 30-31 0.9964814916146096 1101 32-33 0.684785665175184 1101 34-35 0.5603100672464691 1101 36-37 0.8709902432824137 1101 38-39 0.9119086955593332 1101 40-41 1.066038831097508 1101 42-43 1.0149973697259256 1101 44-45 0.9490758745236292 1101 46-47 1.5862108651939977 1101 48-49 1.278704399327026 1101 50-51 1.3145235468609968 1101 52-53 1.3294449657120282 1101 54-55 1.4710240113727622 1101 56-57 1.5182423036338122 1101 58-59 1.4662960672376002 1101 60-61 1.3352852329301683 1101 62-63 1.2645318982337912 1101 64-65 1.180269285121522 1101 66-67 1.378298521387027 1101 68-69 1.3351900662444258 1101 70-71 1.4377841793871937 1101 72-73 1.422699639500415 1101 74-75 1.5558697042958265 1101 76-77 1.487314999696224 1101 78-79 1.5375103533463914 1101 80-81 1.433161010525371 1101 82-83 1.3195978822031087 1101 84-85 1.7306028557093214 1101 86-87 1.8325226565573551 1101 88-89 2.0504593298407414 1101 90-91 1.7869059799901912 1101 92-93 1.8903928810241872 1101 94-95 2.104212576831472 1101 96-97 2.132407650810066 1101 98-99 2.4620365793693573 1101 100-101 2.888992446050075 1102 1 0.23794269988954042 1102 2 0.24712618477784076 1102 3 0.1999047856536862 1102 4 0.08590313697644092 1102 5 0.17120233050889766 1102 6 0.19075360941295827 1102 7 0.13907680985368387 1102 8 0.07946585814865159 1102 9 0.128923478086989 1102 10-11 0.24154337349580857 1102 12-13 0.3643325967621678 1102 14-15 0.5170139460156662 1102 16-17 0.45448546023851577 1102 18-19 0.4927890139128053 1102 20-21 0.3525796972772852 1102 22-23 0.4451467522704533 1102 24-25 0.46561463004435666 1102 26-27 0.4738612943148439 1102 28-29 0.33538768040808975 1102 30-31 0.4626831830991236 1102 32-33 0.5493269925250388 1102 34-35 0.44985230990179303 1102 36-37 0.5962678423576904 1102 38-39 0.26426599943551565 1102 40-41 0.4724527688462743 1102 42-43 0.6618646713530723 1102 44-45 0.5953547654872153 1102 46-47 0.4877959127637226 1102 48-49 0.6784653738396926 1102 50-51 0.40809391816119955 1102 52-53 0.7539074198813793 1102 54-55 0.8610304446626813 1102 56-57 0.8830673756814278 1102 58-59 0.749411819383333 1102 60-61 0.8956156320556588 1102 62-63 0.9324224590806764 1102 64-65 0.7865009463225014 1102 66-67 0.6883674046620492 1102 68-69 0.8813515365911471 1102 70-71 0.5788353684997638 1102 72-73 0.6049631114894503 1102 74-75 0.42044764386474043 1102 76-77 0.37926057301742233 1102 78-79 0.5703462835321211 1102 80-81 0.7694050152744971 1102 82-83 0.8787854570620226 1102 84-85 0.7988689533174984 1102 86-87 0.8158314074007436 1102 88-89 0.7836150939116031 1102 90-91 1.0133041744847553 1102 92-93 1.322120245519752 1102 94-95 1.156327455514269 1102 96-97 1.1583225640129555 1102 98-99 1.4887164264348876 1102 100-101 1.691040638190998 1103 1 0.28283398356624545 1103 2 0.37582602629923656 1103 3 0.21296342273767976 1103 4 0.16051486439324236 1103 5 0.1587573225849681 1103 6 0.27852793587888414 1103 7 0.182662229821986 1103 8 0.11619485973343302 1103 9 0.2576816397351678 1103 10-11 0.1806482863325769 1103 12-13 0.4129462259222336 1103 14-15 0.2349435815148695 1103 16-17 0.2338337962131618 1103 18-19 0.3313969378430741 1103 20-21 0.07624245480501912 1103 22-23 0.07347004862543827 1103 24-25 0.3425857869381801 1103 26-27 0.012167474980458337 1103 28-29 -0.009791400416006013 1103 30-31 0.10101218468391693 1103 32-33 0.36740876748541496 1103 34-35 0.4635562718669348 1103 36-37 0.4753968439424767 1103 38-39 0.5894070453943172 1103 40-41 0.5991266198763867 1103 42-43 0.3843280627952268 1103 44-45 0.3563550824444235 1103 46-47 0.42221746585405384 1103 48-49 0.5358713960266925 1103 50-51 0.5146397184781506 1103 52-53 0.3676773089463481 1103 54-55 0.5483863876103854 1103 56-57 0.7032271221156563 1103 58-59 0.7889376513960187 1103 60-61 0.678024506857561 1103 62-63 1.1644896540093654 1103 64-65 0.7127564138343807 1103 66-67 0.907637135248418 1103 68-69 1.1547055777955855 1103 70-71 0.9731161925885168 1103 72-73 1.0598296725037137 1103 74-75 1.0698168990152972 1103 76-77 1.0768212703232898 1103 78-79 1.1606892312341834 1103 80-81 1.2495596903933581 1103 82-83 1.5126922716420559 1103 84-85 1.7944391593396851 1103 86-87 2.034435527844483 1103 88-89 2.1817108149892555 1103 90-91 2.081996409033092 1103 92-93 2.367191560892177 1103 94-95 2.165992114785265 1103 96-97 2.33690006005099 1103 98-99 2.475435919303351 1103 100-101 2.2454888156870396 1104 1 0.16114133880881099 1104 2 0.23369394492208784 1104 3 0.26216530067195265 1104 4 0.34592957487837594 1104 5 0.23281553854740622 1104 6 0.2729467152216074 1104 7 0.26168320008802937 1104 8 0.2355313229572218 1104 9 0.4263176334753851 1104 10-11 0.4490226212308528 1104 12-13 0.300412892588902 1104 14-15 0.5937880259593129 1104 16-17 0.7743233110801384 1104 18-19 0.7489080489541848 1104 20-21 0.5590706238191032 1104 22-23 0.5847877325065056 1104 24-25 0.6257983064999948 1104 26-27 0.5477968959507251 1104 28-29 0.5497578953586455 1104 30-31 0.674304203463258 1104 32-33 0.6791108019142129 1104 34-35 0.9734517335257777 1104 36-37 0.918999973832932 1104 38-39 1.137582319259728 1104 40-41 1.098073724727719 1104 42-43 1.0621471551270005 1104 44-45 1.0768358336181336 1104 46-47 1.2770218477006878 1104 48-49 1.2913944007215292 1104 50-51 1.0051912051761178 1104 52-53 0.6626582166145809 1104 54-55 0.8123137741518534 1104 56-57 0.7255820516931237 1104 58-59 0.9944224714273133 1104 60-61 0.9866317056056033 1104 62-63 1.2325434881251667 1104 64-65 1.2217442072616187 1104 66-67 1.3716358832922317 1104 68-69 1.2223087076860395 1104 70-71 0.9770085243569007 1104 72-73 1.277802755445812 1104 74-75 0.9828247237414303 1104 76-77 1.3540118806519281 1104 78-79 0.974176555177845 1104 80-81 1.2532930237266946 1104 82-83 1.4102058866655263 1104 84-85 1.6366751530799029 1104 86-87 1.6744593150119336 1104 88-89 1.730021300122278 1104 90-91 1.8895532165448294 1104 92-93 2.107629745555709 1104 94-95 1.8815952446757223 1104 96-97 2.0654984951057607 1104 98-99 2.3294578285365297 1104 100-101 2.1766324776588704 1105 1 0.4355345752822224 1105 2 0.2274804641690622 1105 3 0.39633620380276824 1105 4 0.36513024900862945 1105 5 0.38915495572106096 1105 6 0.19897527315699648 1105 7 0.27101962627168774 1105 8 0.2707865757097636 1105 9 0.4883112255339839 1105 10-11 0.40825302006038555 1105 12-13 0.45401249811158095 1105 14-15 0.5886926939408994 1105 16-17 0.4449509559764735 1105 18-19 0.6133886538194062 1105 20-21 0.6951548808405263 1105 22-23 0.7812925338325414 1105 24-25 0.594235491080191 1105 26-27 0.7822041613709914 1105 28-29 0.64829735698045 1105 30-31 0.4979388119028485 1105 32-33 0.43725847162743037 1105 34-35 0.8285692896184074 1105 36-37 0.7883282048892255 1105 38-39 0.7363833767552634 1105 40-41 0.6591633062669189 1105 42-43 0.7457446308425659 1105 44-45 0.6790249049296264 1105 46-47 0.5131642114161892 1105 48-49 0.7437897392219668 1105 50-51 0.6752042155195781 1105 52-53 0.6292086698930959 1105 54-55 0.7597272160127488 1105 56-57 0.6223442818789735 1105 58-59 0.7112796632303358 1105 60-61 0.8922256902895143 1105 62-63 0.7599890622933856 1105 64-65 0.8370593724142701 1105 66-67 0.7836679044791914 1105 68-69 0.5295067612275375 1105 70-71 0.958359979570762 1105 72-73 1.2637101458764946 1105 74-75 1.135065419725354 1105 76-77 1.1143703827493248 1105 78-79 0.9246620122992688 1105 80-81 1.3504330631744246 1105 82-83 1.1762141651331781 1105 84-85 1.011538567623706 1105 86-87 1.2807621550634174 1105 88-89 1.5497782706105596 1105 90-91 1.4485928587372356 1105 92-93 1.6538187798270876 1105 94-95 1.55121933372018 1105 96-97 1.3553166280983255 1105 98-99 1.3407909488891505 1105 100-101 1.3911503541485786 1106 1 0.20228013741239792 1106 2 -0.2934662813930693 1106 3 -0.019344269570012784 1106 4 0.14666871054708963 1106 5 0.20755732258496806 1106 6 -0.006764371813417824 1106 7 0.30149299905275484 1106 8 0.18173332127189923 1106 9 0.3294354858890145 1106 10-11 -0.18778248289819288 1106 12-13 -0.029330697154691165 1106 14-15 -0.02373334156204976 1106 16-17 0.0628799500593189 1106 18-19 0.24395078399691528 1106 20-21 0.1948886086511763 1106 22-23 0.43531620247159 1106 24-25 0.38240117155356046 1106 26-27 0.44235209036507683 1106 28-29 0.467439368814766 1106 30-31 0.6180275692992936 1106 32-33 0.6104241521008049 1106 34-35 0.553894733405393 1106 36-37 0.6254583824040196 1106 38-39 0.5561762761635478 1106 40-41 0.803157389107156 1106 42-43 0.39038960125676425 1106 44-45 0.4096166209059646 1106 46-47 0.6883713120079022 1106 48-49 0.6871329344882326 1106 50-51 0.42931664155507576 1106 52-53 0.5859542320232762 1106 54-55 0.7601710029949942 1106 56-57 0.7941194298079708 1106 58-59 0.7267530360113952 1106 60-61 0.7264860453190991 1106 62-63 0.7720896540093634 1106 64-65 0.9461717984497717 1106 66-67 1.4121294429407278 1106 68-69 1.342613270103275 1106 70-71 1.1963777310500525 1106 72-73 1.0953373648114066 1106 74-75 1.1347399759383734 1106 76-77 1.221559731861749 1106 78-79 1.240135385080336 1106 80-81 0.8499596903933586 1106 82-83 1.0165691947189792 1106 84-85 1.164408390108914 1106 86-87 1.1604662970752493 1106 88-89 1.4822338919123332 1106 90-91 1.4727348705715535 1106 92-93 1.4971915608921726 1106 94-95 1.330361345554497 1106 96-97 1.1741923677432986 1106 98-99 1.1577436116110427 1106 100-101 0.5603811233793472 1107 1 0.31376332488361314 1107 2 0.23710027779624454 1107 3 0.31817300357600686 1107 4 0.24249091229743414 1107 5 0.19659923875263274 1107 6 0.294101588573497 1107 7 0.5131005531752777 1107 8 0.23157210524241378 1107 9 0.2171139750645068 1107 10-11 0.26566984321880227 1107 12-13 0.27521329179050014 1107 14-15 0.5138645395986998 1107 16-17 0.5587942752550745 1107 18-19 0.46788675820234715 1107 20-21 0.5518807781583135 1107 22-23 0.4197035815595669 1107 24-25 0.5121570444232049 1107 26-27 0.34915310372296915 1107 28-29 0.6161427313205223 1107 30-31 0.6806576936659496 1107 32-33 0.46670697107822434 1107 34-35 0.5882245353399824 1107 36-37 0.6218770834634384 1107 38-39 0.4275926741368252 1107 40-41 0.5112918893374641 1107 42-43 0.5202813562084074 1107 44-45 0.5592688548995142 1107 46-47 0.7367983041774053 1107 48-49 0.5534618151883706 1107 50-51 0.39614989811887114 1107 52-53 0.6401527580481527 1107 54-55 0.5835588427002065 1107 56-57 0.7414271221156596 1107 58-59 0.739173579539731 1107 60-61 0.8904496565581539 1107 62-63 0.8742752827518743 1107 64-65 0.7229635994631245 1107 66-67 0.9276107879430278 1107 68-69 0.9768325239033686 1107 70-71 0.9353114021693507 1107 72-73 0.9722811695096993 1107 74-75 1.0749725876380474 1107 76-77 1.3913386355927493 1107 78-79 1.322546716264121 1107 80-81 1.2814866365011426 1107 82-83 1.3054084393067242 1107 84-85 1.3413936503576487 1107 86-87 1.5420738511977774 1107 88-89 1.3931551263665014 1107 90-91 1.5325137743025543 1107 92-93 1.6444071297544518 1107 94-95 1.9287837315517358 1107 96-97 1.7705719163384153 1107 98-99 1.9494478953512555 1107 100-101 1.9342756420343434 1108 1 0.02054324001541019 1108 2 0.08924878805947856 1108 3 0.08495128313221656 1108 4 0.13453610870280386 1108 5 0.04722348343474181 1108 6 0.2109734593993693 1108 7 0.019515644086020245 1108 8 0.02717513287456086 1108 9 0.23988255020557858 1108 10-11 0.10463493276656521 1108 12-13 0.022972326073976035 1108 14-15 0.1948671020004511 1108 16-17 0.2558016262586804 1108 18-19 0.1992536904986082 1108 20-21 0.003288281815649441 1108 22-23 0.08104516243424342 1108 24-25 0.20613176569386127 1108 26-27 0.34883393932036455 1108 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2.2479352945602784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2221.0 3 329.0 4 349.0 5 378.0 6 461.0 7 587.0 8 748.0 9 1012.0 10 1320.0 11 1871.0 12 2823.0 13 4172.0 14 4748.0 15 6593.0 16 9004.0 17 12526.0 18 9797.0 19 5611.0 20 5424.0 21 5672.0 22 6183.0 23 6865.0 24 7806.0 25 8924.0 26 10104.0 27 12001.0 28 13948.0 29 16297.0 30 20060.0 31 24135.0 32 29463.0 33 37626.0 34 48828.0 35 66965.0 36 82773.0 37 97359.0 38 62922.0 39 5695.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.177126549038164 19.51586275232959 15.214566221826855 19.092444476805383 2 14.755901126269386 22.184405646853314 40.87291415479163 22.18677907208567 3 19.388836850166456 29.1652953760079 30.09281528081923 21.353052493006416 4 11.60634747839791 17.34259073194736 38.390092389386695 32.66096940026804 5 11.859735064967943 40.35417497357612 32.47131347269954 15.3147764887564 6 28.09478765944523 37.824901295808864 18.268184349058515 15.812126695687386 7 26.769789020458912 36.1808242406603 20.264191055277337 16.78519568360345 8 27.369491697011306 32.757022503013374 19.591668222125907 20.281817577849417 9 28.77016802003395 16.780805123021164 20.578617904313532 33.87040895263135 10-11 24.739058244493823 28.348775600769198 25.805132375287837 21.107033779449143 12-13 27.442543282373187 26.50926711045864 26.88268831629037 19.165501290877796 14-15 23.784433600392283 26.274725361636836 28.297360782669905 21.643480255300975 16-17 19.98631816204674 30.44050773063387 28.125430028866305 21.44774407845308 18-19 20.01269984864564 27.64494512811348 33.06989977715957 19.272455246081314 20-21 22.21684464088433 25.85572740091213 32.59249242195983 19.334935536243712 22-23 21.126033262083425 26.572104355417643 32.602013501897495 19.699848880601444 24-25 20.70568400008857 26.668206103127478 33.084097515918934 19.542012380865014 26-27 20.453954168073594 26.581062284068114 33.44541297208474 19.51957057577356 28-29 19.864612308590292 27.214523608218805 33.36367785037438 19.55718623281653 30-31 19.979275595035634 27.0400487260819 33.23435188774017 19.746323791142295 32-33 19.710074087080596 27.28978275329959 32.890329449041104 20.109813710578706 34-35 20.36249484912789 27.01413929223656 32.86473908707169 19.758626771563865 36-37 20.553278413031563 26.790135856177034 32.76546679752218 19.891118933269222 38-39 20.296336828920754 27.069241730070644 32.82415102455873 19.810270416449875 40-41 20.016968960876504 27.415862894591598 32.70375800016131 19.86341014437059 42-43 19.90263545391712 27.6528121999864 31.756879652929236 20.68767269316724 44-45 20.2234476061388 27.576515542720042 31.511205198383298 20.688831652757866 46-47 20.759897914717506 28.16400710832444 30.702140801450135 20.373954175507915 48-49 20.679924103114097 28.400035749679482 30.05717575950273 20.86286438770369 50-51 20.50399149493588 28.764471739962726 30.053045907728915 20.67849085737248 52-53 20.785924431073397 29.25279904690406 29.16926075733472 20.792015764687825 54-55 20.135596652161052 28.89116676078707 29.495435555998473 21.477801031053406 56-57 20.927957819092743 29.030054212713736 28.85443222145609 21.187555746737434 58-59 21.277663967640144 28.419080992143282 29.236932676669486 21.06632236354709 60-61 20.968604022303257 28.81253814989231 29.87947006043892 20.339387767365515 62-63 21.006154140543394 28.851477491881326 29.746646772258845 20.39572159531643 64-65 20.59973481113793 29.170616694563943 29.75365267216916 20.475995822128958 66-67 20.81789177798951 29.019423348343302 29.213459158782808 20.94922571488438 68-69 20.69508107539652 28.967879008798224 29.41391822640701 20.923121689398247 70-71 20.9436892344089 29.267507764711837 29.031798946149905 20.757004054729357 72-73 20.78650574819892 28.893051633763143 29.261768210668983 21.058674407368954 74-75 20.272839127395567 29.929908241791264 28.83338025095127 20.963872379861904 76-77 20.345861938342086 29.28604586976757 29.15666595228285 21.211426239607494 78-79 20.354351818022216 29.28495396621289 29.20255024491778 21.158143970847114 80-81 20.381130565567457 29.869935774462558 28.74594933125416 21.002984328715822 82-83 20.33385942827502 29.52357305972179 28.76843177318244 21.374135738820748 84-85 20.216218609442684 29.164607761827472 28.77174895302218 21.847424675707664 86-87 20.427422734208843 29.193383881909252 28.730144925244506 21.649048458637402 88-89 20.548447804152893 28.807505574620844 28.595511837173625 22.04853478405263 90-91 20.28337468747183 28.682297424024622 28.959678528899662 22.074649359603885 92-93 20.50324640665365 28.93639162157439 28.93987090284977 21.620491068922192 94-95 20.13533890471638 28.693413417022683 29.0519239299837 22.11932374827723 96-97 20.008745869237394 28.614491415051596 29.296685030883356 22.080077684827657 98-99 20.202870105088515 28.85889322155734 28.538147471873458 22.40008920148068 100-101 20.560522285449522 27.815795861471326 29.56483855689943 22.058843296179717 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1644.0 1 1043.5 2 581.0 3 767.5 4 720.5 5 696.5 6 1135.0 7 2306.0 8 3516.0 9 3791.5 10 3166.0 11 2528.0 12 2408.0 13 2441.0 14 2459.5 15 2439.0 16 2376.0 17 2334.5 18 2370.0 19 2404.5 20 2509.0 21 2746.0 22 2960.5 23 3287.0 24 3713.0 25 4186.0 26 4935.0 27 5728.0 28 6352.0 29 7418.0 30 8659.0 31 9586.0 32 11009.0 33 12445.5 34 13872.5 35 15126.0 36 16997.5 37 18933.0 38 19153.5 39 18858.0 40 18846.0 41 19398.5 42 19546.5 43 19969.5 44 20824.5 45 21379.5 46 21336.0 47 22875.0 48 28416.0 49 28152.0 50 22527.0 51 18953.5 52 16315.5 53 15143.0 54 14444.5 55 13454.5 56 12281.5 57 11122.0 58 10288.5 59 9556.5 60 8735.5 61 8004.0 62 6740.5 63 4752.0 64 3133.5 65 2297.0 66 1680.0 67 1257.5 68 884.0 69 558.0 70 412.0 71 342.0 72 302.0 73 260.5 74 192.5 75 142.5 76 102.5 77 60.5 78 33.5 79 22.5 80 15.0 81 13.0 82 12.0 83 9.5 84 7.5 85 4.0 86 2.5 87 2.0 88 0.5 89 3.0 90 4.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2899305555555556 2 0.25284090909090906 3 0.2512626262626263 4 0.2526830808080808 5 0.2518939393939394 6 0.2222222222222222 7 0.22190656565656566 8 0.22380050505050506 9 0.23248106060606058 10-11 0.23903093434343436 12-13 0.23232323232323235 14-15 0.22174873737373738 16-17 0.2170138888888889 18-19 0.2065972222222222 20-21 0.21235795454545453 22-23 0.2080965909090909 24-25 0.2105429292929293 26-27 0.20699179292929293 28-29 0.22340593434343436 30-31 0.23595328282828282 32-33 0.22703598484848483 34-35 0.22498421717171718 36-37 0.21314709595959594 38-39 0.20186237373737373 40-41 0.20028409090909088 42-43 0.21101641414141414 44-45 0.20935921717171718 46-47 0.2183554292929293 48-49 0.22514204545454547 50-51 0.23768939393939395 52-53 0.24534406565656566 54-55 0.2258522727272727 56-57 0.2013888888888889 58-59 0.19120896464646464 60-61 0.19349747474747475 62-63 0.19910037878787876 64-65 0.1926294191919192 66-67 0.1961016414141414 68-69 0.1983112373737374 70-71 0.1976010101010101 72-73 0.2006786616161616 74-75 0.2029671717171717 76-77 0.2136205808080808 78-79 0.21259469696969696 80-81 0.20470328282828285 82-83 0.20012626262626262 84-85 0.186947601010101 86-87 0.19018308080808083 88-89 0.20044191919191917 90-91 0.19839015151515155 92-93 0.20288825757575757 94-95 0.19910037878787876 96-97 0.2054135101010101 98-99 0.20888573232323232 100-101 0.1934185606060606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 633600.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.72910393123516 #Duplication Level Percentage of deduplicated Percentage of total 1 78.59341169472582 42.22753585256276 2 11.883400596751613 12.769689314387387 3 3.9932203997067863 6.43656461628523 4 1.7506101631670414 3.7623486159951396 5 0.9574496986168725 2.572145718295786 6 0.5837245661047238 1.8817798727673511 7 0.3809773966493845 1.4328701898018328 8 0.29067665764004946 1.2494237078981019 9 0.20575842083866108 0.9949694020170519 >10 1.2070879253039983 12.123386431430976 >50 0.0984390757597506 3.615337174498624 >100 0.047603359399580394 4.658777401487653 >500 0.004995414257979269 1.9525247432624622 >1k 0.0020569352826973458 2.2343438317013313 >5k 5.876957950563845E-4 2.08830312760836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7631 1.2043876262626263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5596 0.8832070707070707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4347 0.6860795454545454 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2720 0.4292929292929293 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1861 0.2937184343434343 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1554 0.24526515151515155 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1333 0.21038510101010102 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1250 0.19728535353535354 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1087 0.17155934343434343 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 990 0.15625 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 973 0.15356691919191917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 960 0.15151515151515152 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 865 0.13652146464646464 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 854 0.13478535353535354 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.11963383838383837 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 720 0.11363636363636363 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 693 0.10937500000000001 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 686 0.10827020202020202 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 650 0.10258838383838385 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 637 0.10053661616161616 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 635 0.10022095959595959 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 7.891414141414142E-5 0.0 0.0 0.0 0.0 20-21 1.5782828282828284E-4 0.0 0.0 0.0 0.0 22-23 1.5782828282828284E-4 0.0 0.0 0.0 0.0 24-25 3.156565656565657E-4 0.0 0.0 4.734848484848485E-4 0.0 26-27 3.156565656565657E-4 0.0 0.0 8.680555555555555E-4 0.0 28-29 3.945707070707071E-4 0.0 0.0 0.00220959595959596 0.0 30-31 4.734848484848485E-4 0.0 0.0 0.004261363636363636 0.0 32-33 4.734848484848485E-4 0.0 0.0 0.006313131313131313 0.0 34-35 4.734848484848485E-4 0.0 0.0 0.0078125 0.0 36-37 6.313131313131314E-4 0.0 0.0 0.010337752525252524 0.0 38-39 6.313131313131314E-4 0.0 0.0 0.012784090909090908 0.0 40-41 6.313131313131314E-4 0.0 0.0 0.01680871212121212 0.0 42-43 6.313131313131314E-4 0.0 0.0 0.023121843434343436 0.0 44-45 6.313131313131314E-4 0.0 0.0 0.035274621212121215 0.0 46-47 6.313131313131314E-4 0.0 0.0 0.06691919191919192 0.0 48-49 6.313131313131314E-4 0.0 0.0 0.09154040404040403 0.0 50-51 6.313131313131314E-4 0.0 0.0 0.1264993686868687 0.0 52-53 6.313131313131314E-4 0.0 0.0 0.16848169191919193 0.0 54-55 6.313131313131314E-4 0.0 0.0 0.20991161616161616 0.0 56-57 6.313131313131314E-4 0.0 0.0 0.2597064393939394 0.0 58-59 6.313131313131314E-4 0.0 0.0 0.3381470959595959 0.0 60-61 6.313131313131314E-4 0.0 0.0 0.44120896464646464 0.0 62-63 6.313131313131314E-4 0.0 0.0 0.5795454545454546 0.0 64-65 6.313131313131314E-4 0.0 0.0 0.6946811868686869 0.0 66-67 6.313131313131314E-4 0.0 0.0 0.8545612373737375 0.0 68-69 6.313131313131314E-4 0.0 0.0 1.0620265151515151 0.0 70-71 6.313131313131314E-4 0.0 0.0 1.2212752525252526 0.0 72-73 6.313131313131314E-4 0.0 0.0 1.4616477272727273 0.0 74-75 6.313131313131314E-4 0.0 0.0 1.675268308080808 0.0 76-77 6.313131313131314E-4 0.0 0.0 1.8618213383838382 0.0 78-79 6.313131313131314E-4 0.0 0.0 2.0990372474747474 0.0 80-81 0.0014204545454545455 0.0 0.0 2.3613478535353534 0.0 82-83 0.0014204545454545455 0.0 0.0 2.678582702020202 0.0 84-85 0.0014204545454545455 0.0 0.0 3.072048611111111 0.0 86-87 0.001499368686868687 0.0 0.0 3.5504261363636362 0.0 88-89 0.0015782828282828283 0.0 0.0 4.126736111111111 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 150 0.0 38.01882 1 GTACATG 7605 0.0 26.808142 1 TACATGG 7820 0.0 25.878544 2 ATGGGAG 1900 0.0 25.254519 5 ACATGGG 7855 0.0 24.914587 3 CATGGGG 1505 0.0 24.306673 4 GAGTACT 5070 0.0 23.98472 12-13 GTATCAA 11135 0.0 22.961523 1 GTATATA 435 0.0 22.94239 1 AGTACTT 5245 0.0 22.86749 12-13 GATAGTC 125 2.2528225E-4 22.802277 7 CATGGGA 6160 0.0 22.520262 4 GTACTTT 5480 0.0 22.448511 14-15 TACCTGG 470 0.0 22.236263 2 CGTCACT 110 0.0028676998 21.601603 1 TGGGAGA 990 0.0 21.591358 6 ATGGGAT 2220 0.0 21.186222 5 CATGGGT 495 0.0 21.114887 4 CCTAGAC 115 0.0037210067 20.652603 3 ATGGGGG 810 0.0 20.528362 5 >>END_MODULE