FastQCFastQC Report
Thu 26 May 2016
SRR937271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633600
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96031.515625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74131.1699810606060606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59270.9354482323232324No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29480.46527777777777773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.26815025252525254No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16590.2618371212121212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16400.25883838383838387No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13980.2206439393939394No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.20044191919191917No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12150.19176136363636365No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10580.16698232323232323No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.1545138888888889No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9340.14741161616161616No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9000.14204545454545456No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.13399621212121213No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8280.13068181818181818No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.12973484848484848No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8080.1275252525252525No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7820.12342171717171717No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7330.11568813131313133No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7010.11063762626262626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT1500.041.1845931
CGACCGT404.4949047E-429.68636318-19
GGTATGC1004.9482744E-528.501168
GACCGGT458.945928E-426.38996310-11
GTACATG65500.026.0456871
TACATGG67000.025.2378432
ATGGGAT17000.025.1460955
ACATGGG65950.024.4873143
GTATCAA106200.023.9840761
GGTATCA78750.023.2926771
GTCGGGA2903.6379788E-1222.9301592
CATGGGA50000.022.8941254
AGTACTT65150.022.71197512-13
GAGTACT63600.022.63063812-13
TACCTCC2955.456968E-1222.5432917
ATAAGGC1954.2168176E-721.922243
ATGGGAG15250.021.4909825
GATCCTA1805.1608913E-621.111979
TAGATAG1805.16442E-621.1103065
GTACTTT68250.020.94791814-15
Produced by FastQC (version 0.11.5)