##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937271_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 633600 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1872490530303 33.0 31.0 34.0 31.0 34.0 2 32.34616319444444 34.0 31.0 34.0 31.0 34.0 3 32.3872821969697 34.0 31.0 34.0 30.0 34.0 4 36.05583333333333 37.0 35.0 37.0 35.0 37.0 5 36.00709595959596 37.0 35.0 37.0 35.0 37.0 6 36.07660827020202 37.0 35.0 37.0 35.0 37.0 7 36.01232481060606 37.0 35.0 37.0 35.0 37.0 8 36.00836647727273 37.0 35.0 37.0 35.0 37.0 9 37.66583964646465 39.0 37.0 39.0 35.0 39.0 10-11 37.59487768308081 39.0 37.0 39.0 35.0 39.0 12-13 37.482274305555556 39.0 37.0 39.0 35.0 39.0 14-15 38.56958254419192 40.0 38.0 41.0 34.0 41.0 16-17 38.57097932449495 40.0 38.0 41.0 34.0 41.0 18-19 38.681339962121214 40.0 38.0 41.0 34.5 41.0 20-21 38.64425110479798 40.0 38.0 41.0 34.0 41.0 22-23 38.570651041666665 40.0 38.0 41.0 34.0 41.0 24-25 38.561594065656564 40.0 38.0 41.0 34.0 41.0 26-27 38.36736900252525 40.0 38.0 41.0 34.0 41.0 28-29 38.19852430555555 40.0 37.5 41.0 34.0 41.0 30-31 38.06919349747474 40.0 37.0 41.0 34.0 41.0 32-33 37.8146819760101 40.0 36.0 41.0 33.0 41.0 34-35 37.606921559343434 40.0 36.0 41.0 33.0 41.0 36-37 37.36750789141414 40.0 35.0 41.0 33.0 41.0 38-39 37.20532986111111 40.0 35.0 41.0 33.0 41.0 40-41 36.94280618686869 39.0 35.0 41.0 32.5 41.0 42-43 36.624866635101014 39.0 35.0 41.0 31.5 41.0 44-45 36.11130445075757 39.0 35.0 41.0 30.5 41.0 46-47 35.45147411616162 39.0 35.0 41.0 27.5 41.0 48-49 34.89681502525252 39.0 35.0 40.5 24.0 41.0 50-51 34.5750339330808 39.0 35.0 40.0 23.0 41.0 52-53 34.32206991792929 38.0 34.0 40.0 22.0 41.0 54-55 34.10914457070707 38.0 34.0 40.0 20.5 41.0 56-57 33.81693813131313 38.0 34.0 40.0 19.5 41.0 58-59 33.5609935290404 38.0 34.0 40.0 18.0 41.0 60-61 33.19830808080808 37.0 33.0 40.0 15.0 41.0 62-63 32.838802083333334 36.5 33.0 40.0 10.5 41.0 64-65 32.42348405934344 36.0 32.5 39.0 7.5 41.0 66-67 31.981245265151514 35.5 32.0 39.0 4.5 40.0 68-69 31.540160984848484 35.0 31.0 38.5 2.0 40.0 70-71 31.093529829545453 35.0 31.0 38.0 2.0 40.0 72-73 30.62928740530303 35.0 30.5 37.0 2.0 39.0 74-75 30.16367108585859 34.5 30.0 36.5 2.0 39.0 76-77 28.839181660353535 33.5 28.5 35.0 2.0 37.5 78-79 29.46011284722222 34.0 29.5 36.0 2.0 38.0 80-81 29.253276515151516 34.0 29.0 35.0 2.0 37.0 82-83 28.863319917929292 34.0 29.0 35.0 2.0 37.0 84-85 28.428143150252524 34.0 29.0 35.0 2.0 36.0 86-87 28.08545533459596 34.0 29.0 35.0 2.0 36.0 88-89 27.63624763257576 34.0 27.0 35.0 2.0 35.5 90-91 27.08055476641414 33.0 26.0 35.0 2.0 35.0 92-93 26.61283380681818 33.0 25.0 35.0 2.0 35.0 94-95 26.146080334595958 33.0 24.5 35.0 2.0 35.0 96-97 25.519569917929292 32.5 21.5 34.5 2.0 35.0 98-99 24.62626815025253 32.0 10.0 34.0 2.0 35.0 100-101 21.924394728535354 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28300885121098673 1101 2 0.4929898881571404 1101 3 0.43245856509958713 1101 4 0.34047552292167893 1101 5 0.3423610389972467 1101 6 0.33367128068863394 1101 7 0.3454682569838283 1101 8 0.3826134700041024 1101 9 0.5506625278646524 1101 10-11 0.3637181388979087 1101 12-13 0.4552957242423261 1101 14-15 0.5750966280346717 1101 16-17 0.5242097901611942 1101 18-19 0.45655370979853416 1101 20-21 0.3438623664068885 1101 22-23 0.33333043022901876 1101 24-25 0.06027624629208361 1101 26-27 0.3262947748987841 1101 28-29 0.18851362394830318 1101 30-31 0.2201720803998839 1101 32-33 0.35770506138235447 1101 34-35 0.5649508300807895 1101 36-37 0.7622566571267626 1101 38-39 0.703360594673164 1101 40-41 0.762483536446048 1101 42-43 0.9433785470236202 1101 44-45 0.8153326879055243 1101 46-47 0.595036740188057 1101 48-49 0.7269261073186186 1101 50-51 0.5915101846402493 1101 52-53 0.6068800302991235 1101 54-55 0.5060950810934131 1101 56-57 0.8266163374884101 1101 58-59 0.7552749106596295 1101 60-61 0.34849042690900944 1101 62-63 0.5873666224280782 1101 64-65 0.804703908319226 1101 66-67 0.6319206021282611 1101 68-69 0.7193092588422267 1101 70-71 0.7581112302340713 1101 72-73 0.7509874191834278 1101 74-75 0.908215285932453 1101 76-77 1.0155070261813357 1101 78-79 0.8599741218387855 1101 80-81 0.4782444799341192 1101 82-83 0.21710364416913563 1101 84-85 0.3317197324412895 1101 86-87 0.6109264210704275 1101 88-89 0.8439456207172107 1101 90-91 0.9710012941875483 1101 92-93 1.1926302768624666 1101 94-95 1.1603675361380539 1101 96-97 1.5304062894666295 1101 98-99 2.1702768357699895 1101 100-101 2.93941306380977 1102 1 0.37815354793105627 1102 2 0.35854720458608824 1102 3 0.33049615015762157 1102 4 0.26172002455091814 1102 5 0.22826690375170955 1102 6 0.24640344138489212 1102 7 0.2995853010178635 1102 8 0.11883027710477734 1102 9 0.32815176648740874 1102 10-11 0.25181158374160617 1102 12-13 0.1366321225414211 1102 14-15 0.32892129734788256 1102 16-17 0.3965429822759674 1102 18-19 0.41322314853321274 1102 20-21 0.4614882732130994 1102 22-23 0.5327048843146756 1102 24-25 0.5031651072859447 1102 26-27 0.3068902987934834 1102 28-29 0.3258774395691617 1102 30-31 0.3320498688126392 1102 32-33 0.4218941268816039 1102 34-35 0.39941129672967435 1102 36-37 0.4278890286047954 1102 38-39 0.1941879262177295 1102 40-41 0.1264900638816684 1102 42-43 0.20784821903043849 1102 44-45 0.12606582144074707 1102 46-47 -0.03336492723700957 1102 48-49 0.08584484388323688 1102 50-51 -0.014632561171573855 1102 52-53 0.0929281596762479 1102 54-55 0.2962390717206844 1102 56-57 0.41708513080197207 1102 58-59 0.17268035960388772 1102 60-61 0.2717734445927107 1102 62-63 0.1442803169671052 1102 64-65 0.1856684310789518 1102 66-67 0.3324666472240594 1102 68-69 0.3974617016617863 1102 70-71 0.1917476604381072 1102 72-73 -0.11176383216991681 1102 74-75 -0.1543118463370341 1102 76-77 -0.031454682667838796 1102 78-79 -0.007881918915845887 1102 80-81 0.19341972170552424 1102 82-83 0.12466955923924772 1102 84-85 0.34560230136379033 1102 86-87 0.2224943132003716 1102 88-89 0.6589336171396738 1102 90-91 0.6318963060342178 1102 92-93 0.33857181237893386 1102 94-95 0.2835009667552839 1102 96-97 0.09100997265587196 1102 98-99 0.8464927537167739 1102 100-101 1.6933972577917693 1103 1 0.19006099642550822 1103 2 0.23405655482380183 1103 3 0.22212721196427765 1103 4 0.17348674404378528 1103 5 0.11222506539989041 1103 6 0.16943101666223015 1103 7 0.10258244840296982 1103 8 0.03192568122522488 1103 9 0.12169091070293803 1103 10-11 0.2047966867526938 1103 12-13 0.2450264173116281 1103 14-15 0.3768312815000243 1103 16-17 0.24269955913809582 1103 18-19 0.13729034346190616 1103 20-21 0.11034025419566973 1103 22-23 0.06394165135112928 1103 24-25 0.19799109777722634 1103 26-27 0.0037257980010920733 1103 28-29 -0.09162366978107883 1103 30-31 0.027967459937833894 1103 32-33 0.16503707458367245 1103 34-35 0.3731330082986162 1103 36-37 0.3844177132323736 1103 38-39 0.34704244285797614 1103 40-41 0.3428082889212902 1103 42-43 0.5777930684757635 1103 44-45 0.6446369783345673 1103 46-47 0.6581786543794763 1103 48-49 0.8308350182097044 1103 50-51 0.8188672803498207 1103 52-53 0.6979348157776712 1103 54-55 0.5301294045257592 1103 56-57 0.5196486807227316 1103 58-59 0.4281884420127611 1103 60-61 0.6593537932456428 1103 62-63 0.5302682725930978 1103 64-65 0.5226484627746757 1103 66-67 0.5609027803460833 1103 68-69 0.4406769156079058 1103 70-71 0.7038947285839079 1103 72-73 0.6183458350250142 1103 74-75 0.674830467450608 1103 76-77 0.6357934948282029 1103 78-79 0.4936876531919161 1103 80-81 0.7116675822443526 1103 82-83 0.7304673405387732 1103 84-85 0.6381157720452499 1103 86-87 0.7541000184301687 1103 88-89 0.9766426504201817 1103 90-91 1.050391393197451 1103 92-93 1.214594633298109 1103 94-95 1.1354553249169328 1103 96-97 1.0944432531630035 1103 98-99 1.520366604746883 1103 100-101 2.3003741199153787 1104 1 0.17222312475102086 1104 2 0.14948441084884223 1104 3 0.14944364388915687 1104 4 0.12483071900466314 1104 5 0.07495683378799356 1104 6 0.168902691153626 1104 7 0.22055928721361084 1104 8 0.1878242727745203 1104 9 0.19051594982656894 1104 10-11 0.2614815067839942 1104 12-13 0.3400824423507558 1104 14-15 0.31286758822928107 1104 16-17 0.2262814057734559 1104 18-19 0.31428314471385477 1104 20-21 0.3625681102207068 1104 22-23 0.20297514117898885 1104 24-25 0.2373513481684668 1104 26-27 0.35092329408873724 1104 28-29 0.10587366981203417 1104 30-31 0.29329453349026124 1104 32-33 0.35545835783875646 1104 34-35 0.154338642101429 1104 36-37 0.11264556925740976 1104 38-39 0.38295355396909514 1104 40-41 0.2463150181857685 1104 42-43 0.17975112794368187 1104 44-45 0.13679722872580413 1104 46-47 0.268482253753497 1104 48-49 0.1857533280688557 1104 50-51 0.4077724446690709 1104 52-53 0.038928868985806275 1104 54-55 -0.1616252120626669 1104 56-57 0.013898445980949248 1104 58-59 0.02673867675454744 1104 60-61 0.29396912970887 1104 62-63 0.424682044111087 1104 64-65 0.4321733454037755 1104 66-67 0.4263162388750388 1104 68-69 0.5774105619302823 1104 70-71 0.5269757927466614 1104 72-73 0.5464134406588137 1104 74-75 0.5275578539920751 1104 76-77 0.3336363743274191 1104 78-79 0.38261159685389146 1104 80-81 0.5183442645604686 1104 82-83 0.4921737568142035 1104 84-85 0.4450891679763913 1104 86-87 0.38289375864925645 1104 88-89 0.5622864688865334 1104 90-91 0.8610367766090299 1104 92-93 0.9293088742996751 1104 94-95 1.0542960134928308 1104 96-97 1.1050510778891365 1104 98-99 1.4314251336983723 1104 100-101 2.0452792842346277 1105 1 0.10623614435450435 1105 2 0.12415359624392153 1105 3 0.13815798119504308 1105 4 0.14055656652899273 1105 5 0.06842388196793081 1105 6 0.1100250995024652 1105 7 0.1219978330183551 1105 8 0.1748440244204872 1105 9 0.16881043733015844 1105 10-11 0.0937233139716298 1105 12-13 0.11651635814003214 1105 14-15 0.2581697430384864 1105 16-17 0.23329009168246984 1105 18-19 0.319931763580243 1105 20-21 0.2440420293435963 1105 22-23 0.17779017206118652 1105 24-25 0.28127630487782795 1105 26-27 0.07291633054547475 1105 28-29 0.27193727696448633 1105 30-31 0.1761863948490756 1105 32-33 0.2799080805008316 1105 34-35 0.3593661443932845 1105 36-37 0.4090603167826643 1105 38-39 0.37539273871596635 1105 40-41 0.047730182412415445 1105 42-43 -0.09853710903902169 1105 44-45 -0.26454408675419216 1105 46-47 0.1436165242019527 1105 48-49 -0.035296342737041186 1105 50-51 -0.08235520485727221 1105 52-53 -0.20325334990279487 1105 54-55 -0.2764102404446618 1105 56-57 -0.028945402117500407 1105 58-59 -0.013257711833389862 1105 60-61 0.1966508346657605 1105 62-63 0.15490377555168067 1105 64-65 0.1200709479817661 1105 66-67 -0.041119704861010575 1105 68-69 0.16954555466115906 1105 70-71 0.3386568587614214 1105 72-73 0.23159553916702436 1105 74-75 0.5459772130127369 1105 76-77 0.5547497078459536 1105 78-79 0.32738824490789753 1105 80-81 0.4355989431910956 1105 82-83 0.26678923402989696 1105 84-85 0.40084358269613674 1105 86-87 0.5022254622171474 1105 88-89 0.6382071474615998 1105 90-91 0.6390032275169766 1105 92-93 0.47228930785432155 1105 94-95 0.8437286976979976 1105 96-97 1.204037336003239 1105 98-99 1.0864577290072432 1105 100-101 1.4249741199153796 1106 1 0.15215330411781736 1106 2 0.18427193943919207 1106 3 0.17585028888735366 1106 4 0.08499443635147941 1106 5 0.15031737309219295 1106 6 0.09410793973915332 1106 7 0.20430552532604196 1106 8 0.05087952737907386 1106 9 0.16833706454909247 1106 10-11 -0.07772639017037619 1106 12-13 -0.08854281345760029 1106 14-15 0.23470820457693975 1106 16-17 0.16805340529193558 1106 18-19 0.22365957423112803 1106 20-21 0.12274025419566925 1106 22-23 0.20400318981266707 1106 24-25 0.25571417470030866 1106 26-27 0.18037195184724908 1106 28-29 0.17326863791122804 1106 30-31 0.2608905368609129 1106 32-33 0.40322168996828367 1106 34-35 -0.1386516070859969 1106 36-37 0.3135869440016066 1106 38-39 0.5556885967041367 1106 40-41 0.4277005966135974 1106 42-43 0.47628537616807165 1106 44-45 0.5920831321807256 1106 46-47 0.795717115917931 1106 48-49 0.7221888643635452 1106 50-51 0.4401595880421283 1106 52-53 0.6101194311622891 1106 54-55 0.5154832506796012 1106 56-57 0.6869717576458072 1106 58-59 0.7975115189358419 1106 60-61 0.8013845624764144 1106 62-63 0.8977144264392507 1106 64-65 0.7140946166208266 1106 66-67 0.7352412418845446 1106 68-69 0.8143384540694392 1106 70-71 1.0192485747377589 1106 72-73 0.9997612196403978 1106 74-75 1.0069535443736832 1106 76-77 1.0009627255974358 1106 78-79 1.1091338070380736 1106 80-81 1.1600368130135834 1106 82-83 0.9525288790003117 1106 84-85 1.1775003874298662 1106 86-87 1.2499769415070894 1106 88-89 1.2703964965740262 1106 90-91 1.5142990855051437 1106 92-93 1.552348479451954 1106 94-95 1.2951784018400083 1106 96-97 1.5861970993168448 1106 98-99 1.2356589124391917 1106 100-101 2.0268971968384584 1107 1 0.08440111618599389 1107 2 0.12672242308727988 1107 3 0.21623260118583687 1107 4 0.23861488775636275 1107 5 0.18472087378312096 1107 6 0.21546215438678473 1107 7 0.10714292744488318 1107 8 0.1649053219437846 1107 9 0.274610671181982 1107 10-11 0.15219069872873803 1107 12-13 0.25623839335953136 1107 14-15 0.2569606228173811 1107 16-17 0.4151630321919768 1107 18-19 0.4224412416654886 1107 20-21 0.3068719907226196 1107 22-23 0.3205416513511281 1107 24-25 0.28513840316644945 1107 26-27 0.2345796902166697 1107 28-29 0.2699176475841938 1107 30-31 0.40450638209352263 1107 32-33 0.2362203081166072 1107 34-35 0.21382881668184694 1107 36-37 0.031500347962911235 1107 38-39 0.310764598546605 1107 40-41 0.2959364326338658 1107 42-43 0.5212277990146887 1107 44-45 0.3754525471968364 1107 46-47 0.6032505106668964 1107 48-49 0.4604960960540154 1107 50-51 0.5733954240623973 1107 52-53 0.7310821211668923 1107 54-55 0.8208862907533003 1107 56-57 0.7151684412017758 1107 58-59 0.6563070048870188 1107 60-61 0.854159781269594 1107 62-63 1.0454850390601607 1107 64-65 0.9171646304393377 1107 66-67 1.1790021815436873 1107 68-69 0.820278113212698 1107 70-71 0.7180947285839068 1107 72-73 1.0394871523902829 1107 74-75 1.164820886612283 1107 76-77 0.9385767283611379 1107 78-79 1.0544193897188627 1107 80-81 0.9285789594898617 1107 82-83 1.0920912926345814 1107 84-85 1.2584331373147108 1107 86-87 1.3513910363942365 1107 88-89 1.4639432492225772 1107 90-91 1.5725901955926602 1107 92-93 1.5734700823999042 1107 94-95 1.4798720913839993 1107 96-97 1.576689959749828 1107 98-99 1.694463610734907 1107 100-101 2.4811118444662803 1108 1 -0.04531138597206308 1108 2 -0.05506271679986696 1108 3 -0.0912397076108391 1108 4 -0.023034045030563277 1108 5 -0.1266313837655133 1108 6 -0.02799781490074338 1108 7 0.0010395045486433219 1108 8 -0.021070980383271376 1108 9 -0.0494241120588228 1108 10-11 -0.09245733449161264 1108 12-13 0.05395631109008292 1108 14-15 -0.03739755006295553 1108 16-17 -0.21542972766008717 1108 18-19 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100-101 0.5367698945632675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 5.0 5 18.0 6 34.0 7 86.0 8 212.0 9 372.0 10 763.0 11 1371.0 12 2148.0 13 3208.0 14 4167.0 15 5899.0 16 8692.0 17 12811.0 18 10447.0 19 5112.0 20 4069.0 21 3896.0 22 4227.0 23 4416.0 24 5166.0 25 5723.0 26 6780.0 27 8254.0 28 10127.0 29 12438.0 30 15147.0 31 19013.0 32 24090.0 33 31838.0 34 43336.0 35 64112.0 36 89675.0 37 124814.0 38 93498.0 39 7632.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.492111313223184 19.873300995401948 15.936852609772117 18.697735081602747 2 15.254601096276982 22.61888039596022 39.87837733329756 22.24814117446524 3 19.65609217171717 28.67140151515152 30.672506313131315 21.0 4 11.845959595959595 17.72348484848485 38.01136363636364 32.41919191919192 5 11.82878517653372 40.35906501049575 32.537445351094554 15.274704461875977 6 28.255839646464647 38.051609848484844 17.574021464646464 16.118529040404038 7 26.428345959595962 36.27367424242424 20.193339646464647 17.104640151515152 8 27.78203914141414 32.540719696969695 19.366477272727273 20.31076388888889 9 28.40261994949495 16.333964646464647 20.71322601010101 34.5501893939394 10-11 24.779277146464647 28.35929608585859 25.65301452020202 21.208412247474747 12-13 27.522606347297263 26.33392838258668 27.1666816612765 18.976783608839558 14-15 23.764914772727273 25.990767045454543 28.805871212121215 21.43844696969697 16-17 19.742503156565657 29.984611742424246 28.875157828282827 21.397727272727273 18-19 19.623027146464647 26.812736742424242 34.41059027777777 19.153645833333332 20-21 21.573643918595266 24.85560673580077 34.50026396801134 19.07048537759263 22-23 20.63385716788432 25.5214276582749 34.4075177123629 19.43719746147788 24-25 20.349921361996024 25.716402869636102 34.80250536813871 19.131170400229166 26-27 20.12607552062577 25.683546970944075 35.1052773211635 19.085100187266658 28-29 19.58453484095906 26.105584307560697 35.166096098513364 19.143784752966873 30-31 19.63369604931822 25.886226235973986 34.98787483260325 19.492202882104547 32-33 19.527808598182762 26.224157550753713 34.605799567234165 19.642234283829364 34-35 20.023121843434343 25.74865845959596 34.728298611111114 19.499921085858585 36-37 20.38817866161616 25.590041035353533 34.483901515151516 19.53787878787879 38-39 20.050031565656564 26.038352272727273 34.54237689393939 19.369239267676768 40-41 19.816761363636363 26.273674242424246 34.41848169191919 19.4910827020202 42-43 19.63391650777215 26.663341456946952 33.450715341002365 20.252026694278534 44-45 20.08041351010101 26.324810606060606 33.13375946969697 20.461016414141415 46-47 20.662247474747474 27.04971590909091 32.250473484848484 20.03756313131313 48-49 20.627525252525253 27.32378472222222 31.442313762626263 20.606376262626263 50-51 20.364741161616163 27.693497474747474 31.350694444444443 20.59106691919192 52-53 20.64559659090909 28.294981060606062 30.320785984848484 20.738636363636363 54-55 20.130287247474747 27.96212121212121 30.620186237373737 21.2874053030303 56-57 20.846354166666668 28.03527462121212 29.997395833333336 21.12097537878788 58-59 21.14441287878788 27.45793876262626 30.388967803030305 21.008680555555557 60-61 20.822837752525253 27.83325441919192 31.208175505050505 20.13573232323232 62-63 20.98587436868687 27.772017045454543 30.98682133838384 20.255287247474747 64-65 20.598011363636363 28.090356691919194 30.950757575757574 20.36087436868687 66-67 20.819444444444443 28.0652619949495 30.272964015151516 20.842329545454547 68-69 20.682291666666668 28.124526515151516 30.50497159090909 20.688210227272727 70-71 20.888573232323232 28.33270202020202 30.13936237373737 20.639362373737374 72-73 20.776817477327896 28.060931379202586 30.235890896120253 20.926360247349265 74-75 20.415167297979796 29.002130681818183 29.784327651515152 20.79837436868687 76-77 20.237215909090907 28.32512626262626 30.46875 20.96890782828283 78-79 20.189551767676768 28.322679924242422 30.350142045454547 21.137626262626263 80-81 20.208412247474747 28.884232954545453 30.007575757575754 20.89977904040404 82-83 20.213921973877653 28.509987839244438 29.99973958148377 21.276350605394136 84-85 20.10632908195878 28.26803703919987 29.779403060926548 21.8462308179148 86-87 20.05129419191919 28.236032196969695 29.773358585858585 21.939315025252526 88-89 20.096748737373737 27.82804608585859 29.902067550505052 22.173137626262626 90-91 19.98055351453396 27.57604767545836 30.15483790517786 22.288560904829815 92-93 20.004766515700698 27.829980594599157 30.161864244266567 22.003388645433574 94-95 19.645675505050505 27.478298611111114 30.35187815656566 22.524147727272727 96-97 19.49202967171717 27.417140151515152 30.450047348484848 22.64078282828283 98-99 19.61852753522544 27.597488942112303 29.789416782736673 22.994566739925585 100-101 19.34047703421447 27.13551380109657 30.659799200812603 22.864209963876352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1111.0 1 804.5 2 801.0 3 1076.0 4 810.5 5 664.5 6 1303.0 7 2905.0 8 4265.5 9 4520.0 10 3685.5 11 2717.0 12 2520.5 13 2531.5 14 2500.5 15 2408.0 16 2377.0 17 2446.5 18 2472.0 19 2488.5 20 2625.5 21 2765.5 22 2949.5 23 3339.5 24 3790.5 25 4209.5 26 4845.5 27 5557.0 28 6137.0 29 7152.5 30 8513.0 31 9471.0 32 10745.5 33 12084.0 34 13373.5 35 14768.5 36 16657.5 37 18550.5 38 19107.0 39 18902.5 40 18939.0 41 19423.5 42 19602.0 43 19975.0 44 20802.5 45 21610.5 46 21191.5 47 22748.5 48 29503.5 49 28752.0 50 22168.0 51 18748.0 52 16141.0 53 15146.5 54 14366.0 55 13296.5 56 12185.0 57 11086.5 58 10219.0 59 9606.0 60 8715.0 61 7727.5 62 6449.5 63 4591.5 64 3066.5 65 2244.5 66 1606.0 67 1201.5 68 834.0 69 493.0 70 350.5 71 305.5 72 270.5 73 252.0 74 201.0 75 149.5 76 102.0 77 44.5 78 31.5 79 22.5 80 11.5 81 8.0 82 7.5 83 4.0 84 0.5 85 0.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045454545454545456 2 1.5782828282828281E-4 3 0.0 4 0.0 5 0.0015782828282828283 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006155303030303031 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.891414141414141E-5 22-23 4.734848484848485E-4 24-25 7.891414141414141E-5 26-27 0.0018150252525252527 28-29 0.00331439393939394 30-31 7.891414141414141E-5 32-33 1.5782828282828281E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008838383838383838 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.1565656565656563E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.102272727272727E-4 84-85 1.5782828282828281E-4 86-87 0.0 88-89 0.0 90-91 0.010495580808080808 92-93 0.0021306818181818185 94-95 0.0 96-97 0.0 98-99 3.9457070707070706E-4 100-101 0.01286300505050505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 633600.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.447051450778105 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41256316096468 39.8264811987876 2 12.600584624995973 12.965258510241027 3 4.043219401400005 6.24035149711831 4 1.8800008654425135 3.868820050077134 5 1.0318436270309606 2.6542656084509653 6 0.6374255607656855 1.9676199372451977 7 0.4042576563595963 1.4558505112272186 8 0.29045460315252747 1.1954426330002732 9 0.2248736129285448 1.0412175900831484 >10 1.3064492819867726 12.482042806644948 >50 0.10501172307164237 3.6819921816841314 >100 0.055285574928314055 5.156568080892866 >500 0.004632869407400165 1.770762809486839 >1k 0.0024708636839467554 2.047722570518386 >5k 9.265738814800333E-4 3.645604014542045 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9603 1.515625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7413 1.1699810606060606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5927 0.9354482323232324 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2948 0.46527777777777773 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1699 0.26815025252525254 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1659 0.2618371212121212 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1640 0.25883838383838387 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1398 0.2206439393939394 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1270 0.20044191919191917 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1215 0.19176136363636365 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1058 0.16698232323232323 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 979 0.1545138888888889 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 934 0.14741161616161616 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 900 0.14204545454545456 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.13399621212121213 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 828 0.13068181818181818 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 822 0.12973484848484848 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 808 0.1275252525252525 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 782 0.12342171717171717 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 733 0.11568813131313133 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 701 0.11063762626262626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.891414141414142E-5 0.0 24-25 0.0 0.0 0.0 6.313131313131314E-4 0.0 26-27 1.5782828282828284E-4 0.0 0.0 8.680555555555555E-4 0.0 28-29 3.156565656565657E-4 0.0 0.0 0.00220959595959596 0.0 30-31 3.156565656565657E-4 0.0 0.0 0.004261363636363636 0.0 32-33 3.156565656565657E-4 0.0 0.0 0.005997474747474748 0.0 34-35 3.156565656565657E-4 0.0 0.0 0.007654671717171717 0.0 36-37 3.156565656565657E-4 0.0 0.0 0.010337752525252524 0.0 38-39 4.734848484848485E-4 0.0 0.0 0.012941919191919192 0.0 40-41 4.734848484848485E-4 0.0 0.0 0.017124368686868688 0.0 42-43 4.734848484848485E-4 0.0 0.0 0.0234375 0.0 44-45 6.313131313131314E-4 0.0 0.0 0.03566919191919192 0.0 46-47 7.891414141414141E-4 0.0 0.0 0.06818181818181818 0.0 48-49 7.891414141414141E-4 0.0 0.0 0.09288194444444445 0.0 50-51 7.891414141414141E-4 0.0 0.0 0.12878787878787878 0.0 52-53 7.891414141414141E-4 0.0 0.0 0.1709280303030303 0.0 54-55 7.891414141414141E-4 0.0 0.0 0.2126736111111111 0.0 56-57 7.891414141414141E-4 0.0 0.0 0.2628630050505051 0.0 58-59 7.891414141414141E-4 0.0 0.0 0.3416982323232323 0.0 60-61 7.891414141414141E-4 0.0 0.0 0.4454703282828283 0.0 62-63 7.891414141414141E-4 0.0 0.0 0.5850694444444444 0.0 64-65 7.891414141414141E-4 0.0 0.0 0.7007575757575757 0.0 66-67 0.0011047979797979798 0.0 0.0 0.860479797979798 0.0 68-69 0.0011047979797979798 0.0 0.0 1.0695233585858586 0.0 70-71 0.0011047979797979798 0.0 0.0 1.2302714646464645 0.0 72-73 0.0011047979797979798 0.0 0.0 1.4738005050505052 0.0 74-75 0.0011047979797979798 0.0 0.0 1.6913667929292928 0.0 76-77 0.0011047979797979798 0.0 0.0 1.8821022727272727 0.0 78-79 0.0011047979797979798 0.0 0.0 2.124368686868687 0.0 80-81 0.001341540404040404 0.0 0.0 2.392440025252525 0.0 82-83 0.0014204545454545455 0.0 0.0 2.7151199494949494 0.0 84-85 0.0014204545454545455 0.0 0.0 3.116635101010101 0.0 86-87 0.0014204545454545455 0.0 0.0 3.6070075757575757 0.0 88-89 0.0014204545454545455 0.0 0.0 4.197916666666666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 150 0.0 41.184593 1 CGACCGT 40 4.4949047E-4 29.686363 18-19 GGTATGC 100 4.9482744E-5 28.50116 8 GACCGGT 45 8.945928E-4 26.389963 10-11 GTACATG 6550 0.0 26.045687 1 TACATGG 6700 0.0 25.237843 2 ATGGGAT 1700 0.0 25.146095 5 ACATGGG 6595 0.0 24.487314 3 GTATCAA 10620 0.0 23.984076 1 GGTATCA 7875 0.0 23.292677 1 GTCGGGA 290 3.6379788E-12 22.930159 2 CATGGGA 5000 0.0 22.894125 4 AGTACTT 6515 0.0 22.711975 12-13 GAGTACT 6360 0.0 22.630638 12-13 TACCTCC 295 5.456968E-12 22.543291 7 ATAAGGC 195 4.2168176E-7 21.92224 3 ATGGGAG 1525 0.0 21.490982 5 GATCCTA 180 5.1608913E-6 21.11197 9 TAGATAG 180 5.16442E-6 21.110306 5 GTACTTT 6825 0.0 20.947918 14-15 >>END_MODULE