FastQCFastQC Report
Thu 26 May 2016
SRR937270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620937
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79301.2771021858900338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56190.9049227216287642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43510.7007152094334852No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27310.43981917650260816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19390.3122700048475127No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15960.25703090651708627No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.21242090582458445No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12540.2019528551205678No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10070.16217426244530445No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9830.15830913603151367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.1515451648073798No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8870.14284863037635057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8350.13447418981313725No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8250.13286372047405776No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7420.119496824959698No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7270.11708112095107877No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.11675902708326287No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7080.11402122920682774No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6870.11063924359476082No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6420.10339213156890313No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6410.10323108463499517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG459.543204E-442.2924961
GTACACG702.0322911E-433.985041
CGATCCG300.003953255231.66309270-71
TCGACCG404.4968157E-429.68414718-19
GTTCGAC504.9558716E-528.4979316-17
CGACCGT458.9538493E-426.38590818-19
GTATAGA1701.1031807E-725.1889131
GTACATG75400.024.9253671
CATATAG1151.274801E-424.7998123
TACATGG76150.024.5311342
CTTACTA3150.024.1262059
CCAAGAC2602.3646862E-1123.7664873
TAATACC1401.7693474E-523.7645684
GAGTACT52200.023.38526212-13
ACATGGG76750.023.3484443
TAGACTG1852.5227564E-723.1222825
GATAGTC1452.3326818E-522.9302947
CATGGGG16850.022.5657634
GTATCAA114750.022.3901441
ATGGGAG17250.022.318035
Produced by FastQC (version 0.11.5)