##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 620937 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782420760882346 33.0 31.0 34.0 30.0 34.0 2 31.831395133483753 33.0 31.0 34.0 30.0 34.0 3 31.834559705735042 33.0 31.0 34.0 30.0 34.0 4 35.50244549769139 37.0 35.0 37.0 33.0 37.0 5 35.54187944992809 37.0 35.0 37.0 33.0 37.0 6 35.63229441956269 37.0 35.0 37.0 33.0 37.0 7 35.58884878820235 37.0 35.0 37.0 33.0 37.0 8 35.58887777665045 37.0 35.0 37.0 33.0 37.0 9 37.17950130206446 39.0 37.0 39.0 34.0 39.0 10-11 37.05669979402097 39.0 37.0 39.0 33.0 39.0 12-13 36.92933824204388 39.0 37.0 39.0 33.0 39.0 14-15 37.98085554573169 40.0 38.0 41.0 33.0 41.0 16-17 37.9998598891675 40.0 38.0 41.0 33.0 41.0 18-19 38.08466076268607 40.0 38.0 41.0 33.5 41.0 20-21 38.08649911343663 40.0 38.0 41.0 33.0 41.0 22-23 37.936586159304404 40.0 38.0 41.0 33.0 41.0 24-25 37.75985405926849 40.0 37.0 41.0 33.0 41.0 26-27 37.59007516060406 40.0 37.0 41.0 32.5 41.0 28-29 37.61240995463308 40.0 37.0 41.0 33.0 41.0 30-31 37.364292995907796 39.0 36.5 41.0 32.5 41.0 32-33 37.05943920236675 39.0 35.5 41.0 32.0 41.0 34-35 36.87150467760819 39.0 35.0 41.0 31.0 41.0 36-37 36.79035151714264 39.0 35.0 41.0 31.0 41.0 38-39 36.65261612691787 39.0 35.0 41.0 31.0 41.0 40-41 36.42008851139488 39.0 35.0 41.0 30.5 41.0 42-43 36.06890312543784 39.0 35.0 41.0 30.0 41.0 44-45 35.64506705189093 39.0 35.0 41.0 28.0 41.0 46-47 35.048875328737054 39.0 35.0 40.5 25.0 41.0 48-49 34.548298780713665 38.5 35.0 40.0 23.0 41.0 50-51 33.608511008363166 37.5 33.5 39.5 20.5 40.5 52-53 33.66967743909608 38.0 33.5 39.5 20.0 40.5 54-55 33.979447190294664 38.0 34.0 40.0 19.5 41.0 56-57 33.7772060611624 38.0 34.0 40.0 17.5 41.0 58-59 33.54405116783184 38.0 34.0 40.0 14.5 41.0 60-61 33.22943793009597 37.5 33.0 40.0 11.5 41.0 62-63 32.91216178130793 37.0 33.0 40.0 8.0 41.0 64-65 32.54668911660925 36.0 33.0 39.5 7.0 41.0 66-67 32.160207879382284 36.0 32.0 39.0 2.0 41.0 68-69 31.706164232442262 35.0 31.5 39.0 2.0 40.0 70-71 31.24816607804012 35.0 31.0 38.5 2.0 40.0 72-73 30.858260177763604 35.0 31.0 37.5 2.0 40.0 74-75 30.425507740720878 35.0 30.5 37.0 2.0 39.0 76-77 30.00008857581365 35.0 30.0 36.5 2.0 39.0 78-79 29.537614927118213 34.5 29.5 36.0 2.0 38.5 80-81 29.064130499551485 34.0 29.0 35.5 2.0 37.0 82-83 28.584880591750853 34.0 29.0 35.0 2.0 37.0 84-85 28.149313859538083 34.0 27.5 35.0 2.0 36.5 86-87 27.727261380784203 34.0 27.0 35.0 2.0 36.0 88-89 27.299768736602907 34.0 26.0 35.0 2.0 36.0 90-91 26.851158813212933 33.0 25.5 35.0 2.0 35.0 92-93 26.413457403891215 33.0 25.0 35.0 2.0 35.0 94-95 25.893211388594978 33.0 23.5 35.0 2.0 35.0 96-97 25.26503735483632 32.5 19.0 35.0 2.0 35.0 98-99 24.553705126284953 32.0 8.5 34.5 2.0 35.0 100-101 22.758104284331584 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06960552332298064 1101 2 0.14513045926546653 1101 3 -0.019237995173050138 1101 4 0.10679733710859551 1101 5 0.17901583839665136 1101 6 0.2384137132016093 1101 7 0.3075145803812376 1101 8 0.1777331053096276 1101 9 0.15540150443494127 1101 10-11 0.3094306111973424 1101 12-13 0.29997236762221036 1101 14-15 0.21310982647948862 1101 16-17 0.2653021255339283 1101 18-19 0.37355808395142986 1101 20-21 0.4310409051886026 1101 22-23 0.3485888973545954 1101 24-25 0.4058384213248303 1101 26-27 0.3209525479069981 1101 28-29 0.3114647398619468 1101 30-31 0.3498632305703495 1101 32-33 0.17408421788108797 1101 34-35 0.29877030928710013 1101 36-37 0.46094606575262276 1101 38-39 0.38218455682086727 1101 40-41 0.35697775121334985 1101 42-43 0.2223670112219267 1101 44-45 0.21669786098669874 1101 46-47 0.26870995215847415 1101 48-49 0.03415917514915634 1101 50-51 -0.24042329738448842 1101 52-53 -0.20437193572492163 1101 54-55 0.08759146443497201 1101 56-57 0.012655354901305316 1101 58-59 0.2927800073660549 1101 60-61 -0.0651902487973075 1101 62-63 0.1609813076377904 1101 64-65 0.1650668092947356 1101 66-67 0.31198070630359354 1101 68-69 0.6029077256522619 1101 70-71 0.4483396842097669 1101 72-73 0.5058082310158198 1101 74-75 0.33160068717257474 1101 76-77 0.27802939994123577 1101 78-79 0.17137806191607297 1101 80-81 0.14261974166651115 1101 82-83 0.32626867746407484 1101 84-85 0.23585816367871715 1101 86-87 0.5157334624420109 1101 88-89 0.48824434160748353 1101 90-91 0.6864972553395638 1101 92-93 0.5389206592963909 1101 94-95 0.6267738432113781 1101 96-97 0.6383505969296159 1101 98-99 0.6582083695239085 1101 100-101 0.6285288442292618 1102 1 0.2714041853427709 1102 2 0.22573976103207727 1102 3 0.3443200707645353 1102 4 0.11886519754203562 1102 5 0.06539768352744346 1102 6 0.04053084655897976 1102 7 0.23256365300872517 1102 8 0.300324540633909 1102 9 0.13048462745701528 1102 10-11 0.3485284760373091 1102 12-13 0.3902860318452568 1102 14-15 0.6976769858753897 1102 16-17 0.4912865825302859 1102 18-19 0.40368767574728537 1102 20-21 0.32496891864852273 1102 22-23 0.5850238210416521 1102 24-25 0.42510693873059324 1102 26-27 0.30367698006659083 1102 28-29 0.45419097468948877 1102 30-31 0.4216745112657776 1102 32-33 0.41226672799605524 1102 34-35 0.5922296290803857 1102 36-37 0.6869856042696227 1102 38-39 0.9119285779721693 1102 40-41 0.47476707941908813 1102 42-43 0.724244790335014 1102 44-45 0.4582904179710354 1102 46-47 0.38983421606906177 1102 48-49 0.7552563990405119 1102 50-51 0.9501737862993593 1102 52-53 0.9387198401033032 1102 54-55 0.7669869697828844 1102 56-57 0.9246901664223586 1102 58-59 1.0605713385361852 1102 60-61 0.7959692672864946 1102 62-63 0.7320865433866928 1102 64-65 1.0825196394834151 1102 66-67 1.0392570753297576 1102 68-69 1.0016678906965275 1102 70-71 0.7277331865932943 1102 72-73 0.7376213143791972 1102 74-75 0.7040118557019959 1102 76-77 0.9544805476398288 1102 78-79 0.6544129535557026 1102 80-81 0.5580696074678997 1102 82-83 0.6368783798235675 1102 84-85 0.7226486135445178 1102 86-87 1.007160774864392 1102 88-89 0.9164580979668955 1102 90-91 0.9366324554357064 1102 92-93 0.9193537001969254 1102 94-95 0.8504849356281561 1102 96-97 0.754537553625525 1102 98-99 0.866331391596578 1102 100-101 1.2720362351678531 1103 1 0.03610286246182781 1103 2 0.18614642443237273 1103 3 0.3499153671878581 1103 4 0.008316446929590882 1103 5 0.12534868793704845 1103 6 0.26798797541738395 1103 7 0.16577776373875963 1103 8 0.2800746631228819 1103 9 0.0813763472022373 1103 10-11 0.36947899049101096 1103 12-13 0.17304203380508199 1103 14-15 0.3842031985162535 1103 16-17 0.2793684051662453 1103 18-19 0.4589620510535113 1103 20-21 0.25980723320021326 1103 22-23 0.006332003305253409 1103 24-25 0.2621059588187791 1103 26-27 0.205546161840239 1103 28-29 0.34764761359199525 1103 30-31 0.3878700036714662 1103 32-33 0.6613725584713137 1103 34-35 0.48817524397504997 1103 36-37 0.5178038306292478 1103 38-39 0.4007404349050532 1103 40-41 0.244553948600867 1103 42-43 0.2816921200753342 1103 44-45 0.3476436761777961 1103 46-47 0.46680138902350166 1103 48-49 0.765334791985147 1103 50-51 0.7413594795869614 1103 52-53 0.794077507913201 1103 54-55 0.6823519869313444 1103 56-57 0.5692688043449365 1103 58-59 0.6644297412799318 1103 60-61 0.35167235400869146 1103 62-63 0.26268183981001414 1103 64-65 0.38405810102187843 1103 66-67 0.422324199288596 1103 68-69 0.49985015429280466 1103 70-71 0.5409497471028466 1103 72-73 0.5661734946829711 1103 74-75 0.5623239576128256 1103 76-77 0.7758450748323824 1103 78-79 0.5089082989746139 1103 80-81 0.5184591224115529 1103 82-83 0.3760993499362577 1103 84-85 0.4105001569056128 1103 86-87 0.6109971295924694 1103 88-89 0.8019260058551865 1103 90-91 0.7718455862539315 1103 92-93 0.673003871681491 1103 94-95 1.013914626955934 1103 96-97 1.0397898809160715 1103 98-99 0.5561280598964302 1103 100-101 0.9203140401654046 1104 1 0.13158755930809818 1104 2 0.3617405126437099 1104 3 0.19296668803040617 1104 4 0.18046121592761466 1104 5 0.1281090742980382 1104 6 0.019003918626971483 1104 7 0.10379475431248153 1104 8 0.15396503901023095 1104 9 0.27438693721736485 1104 10-11 0.26785790355977923 1104 12-13 0.34253580780717385 1104 14-15 0.5866528668509474 1104 16-17 0.41735281107803246 1104 18-19 0.3044327830446676 1104 20-21 0.41190894389496435 1104 22-23 0.4761364953336411 1104 24-25 0.27431124218897196 1104 26-27 0.3922888593847489 1104 28-29 0.29211869470452 1104 30-31 0.30600220429987957 1104 32-33 0.38144703769859234 1104 34-35 0.5257348855437627 1104 36-37 0.2785858625156692 1104 38-39 0.3239430416779996 1104 40-41 0.2895056534769225 1104 42-43 0.38159902104123944 1104 44-45 0.35521844633955624 1104 46-47 0.21289472080518124 1104 48-49 0.31702104824024246 1104 50-51 0.27416176051330154 1104 52-53 0.041554524089157496 1104 54-55 0.10356925680216733 1104 56-57 0.2563303660812366 1104 58-59 0.19031709144867648 1104 60-61 0.13531950866946119 1104 62-63 -0.18534096945334255 1104 64-65 -0.14440925612929334 1104 66-67 0.03962083608598732 1104 68-69 0.14913794668195735 1104 70-71 0.0656849967246309 1104 72-73 -0.016027133735512678 1104 74-75 0.09146162780949751 1104 76-77 0.3967353343459443 1104 78-79 0.29304073235061523 1104 80-81 0.21635973919265794 1104 82-83 0.30819524194138026 1104 84-85 0.4070741124508288 1104 86-87 0.44927945241336786 1104 88-89 0.5928290664859297 1104 90-91 0.4647456211660703 1104 92-93 0.8642234690281718 1104 94-95 0.8290688222311609 1104 96-97 0.6518462058316992 1104 98-99 1.0763189129163315 1104 100-101 1.489238746321579 1105 1 0.17486496866242973 1105 2 0.26900070473962145 1105 3 0.15672155665685494 1105 4 0.10031689221513318 1105 5 0.08664446885656218 1105 6 0.1989553582516237 1105 7 0.3239409608889332 1105 8 0.18183799385871424 1105 9 0.23040673794029942 1105 10-11 0.14147832256269766 1105 12-13 0.03831644101870779 1105 14-15 0.15523180811216264 1105 16-17 0.29469735985843926 1105 18-19 0.12099366632680386 1105 20-21 0.2259443811463342 1105 22-23 0.13785042699444006 1105 24-25 0.36956226517523305 1105 26-27 0.30913961614280794 1105 28-29 0.3258887357115512 1105 30-31 0.06262342680404487 1105 32-33 0.1468005891960189 1105 34-35 0.2510885246162573 1105 36-37 0.5723958377538167 1105 38-39 0.7138852640267217 1105 40-41 0.5680950818525616 1105 42-43 0.6173817560544066 1105 44-45 0.591135828020164 1105 46-47 0.6120290412554965 1105 48-49 0.6839967089393966 1105 50-51 0.6624849387876779 1105 52-53 0.7888643274612406 1105 54-55 0.9708547143052684 1105 56-57 1.2056397271992196 1105 58-59 1.1704911906843662 1105 60-61 1.0699490989713993 1105 62-63 1.0421363650243123 1105 64-65 1.008713561335803 1105 66-67 0.8752719895591099 1105 68-69 0.7653216003575913 1105 70-71 1.0964490235138449 1105 72-73 1.1861946457460917 1105 74-75 1.147794290463768 1105 76-77 1.0507098193498052 1105 78-79 0.9023792997538642 1105 80-81 1.0727683732186328 1105 82-83 1.1313592853698573 1105 84-85 1.2789583557256066 1105 86-87 1.0761713475597396 1105 88-89 1.1354036859175238 1105 90-91 0.8539773907735793 1105 92-93 0.9157011888361168 1105 94-95 0.8986658236608243 1105 96-97 0.8900514861704387 1105 98-99 1.3002001961538063 1105 100-101 1.2578260072142733 1106 1 0.26090686200733515 1106 2 0.2764952484296437 1106 3 0.0880564875150931 1106 4 0.2774540449298186 1106 5 0.24999815958799587 1106 6 0.22587457921240173 1106 7 0.2328905918264752 1106 8 0.10729656972440438 1106 9 0.09623602223916805 1106 10-11 -0.15746226618437476 1106 12-13 0.007801720204348328 1106 14-15 0.14412252586541996 1106 16-17 0.09420762798183091 1106 18-19 0.18960743226019616 1106 20-21 0.08551681165720026 1106 22-23 0.24348800830468065 1106 24-25 0.09248432491354208 1106 26-27 0.3310728065397015 1106 28-29 0.3335446707445868 1106 30-31 0.40719394413277143 1106 32-33 0.5897556967510553 1106 34-35 0.5003045474632941 1106 36-37 0.4514250100406798 1106 38-39 0.4119391623223905 1106 40-41 0.2749357233991816 1106 42-43 0.5161541130306802 1106 44-45 0.6533623445109384 1106 46-47 1.0249493267578487 1106 48-49 1.109157084906407 1106 50-51 1.0989427087654704 1106 52-53 0.7998143559986488 1106 54-55 0.8361004246088797 1106 56-57 0.6774315131280346 1106 58-59 0.7697757246909092 1106 60-61 0.6267160990376652 1106 62-63 0.6739396514223301 1106 64-65 0.8011311609014342 1106 66-67 0.8976690464650545 1106 68-69 0.9747177829713571 1106 70-71 1.052692730854698 1106 72-73 0.877166563652402 1106 74-75 0.6167109590899322 1106 76-77 0.7416069200772419 1106 78-79 0.7522158776588519 1106 80-81 0.8596612585324515 1106 82-83 0.6252755799101273 1106 84-85 0.4866187797893815 1106 86-87 0.669117797698366 1106 88-89 0.5728736254438687 1106 90-91 0.4889379621203105 1106 92-93 0.7799860327995454 1106 94-95 0.8516421579183238 1106 96-97 0.7624123101854714 1106 98-99 1.0695356272183254 1106 100-101 1.1443101769680375 1107 1 0.08015474081603458 1107 2 0.16177701477226591 1107 3 0.3004654566332938 1107 4 0.19580750238576172 1107 5 0.20850611190842727 1107 6 0.24024200046210353 1107 7 0.3248833093559327 1107 8 0.20256124630714112 1107 9 0.19567867278363593 1107 10-11 0.42048077939977446 1107 12-13 0.29610554006625733 1107 14-15 0.5045296743659833 1107 16-17 0.3983420187619515 1107 18-19 0.304170011697515 1107 20-21 0.040395336956919436 1107 22-23 0.31824389954854126 1107 24-25 0.3125912003214637 1107 26-27 0.42345090244846517 1107 28-29 0.4664669338066645 1107 30-31 0.23956723086287468 1107 32-33 0.4934722901350028 1107 34-35 0.362504403187927 1107 36-37 0.25972019914445355 1107 38-39 0.4557091290016473 1107 40-41 0.5015038591554202 1107 42-43 0.5139148392166604 1107 44-45 0.5532809749255563 1107 46-47 0.8723738398285121 1107 48-49 0.918579425258848 1107 50-51 1.04334516831684 1107 52-53 0.8754236617593563 1107 54-55 0.9454512713678369 1107 56-57 0.9411784644522712 1107 58-59 1.035695394231631 1107 60-61 1.069252854903148 1107 62-63 0.8495691385577828 1107 64-65 1.030194058088064 1107 66-67 1.1546520168198953 1107 68-69 1.2450022115378836 1107 70-71 1.3582462587307553 1107 72-73 1.4153125823394959 1107 74-75 1.122760004124455 1107 76-77 0.9629515149039385 1107 78-79 1.170290230996077 1107 80-81 1.3284971367228238 1107 82-83 1.3998023910811632 1107 84-85 1.2175752910737714 1107 86-87 1.2337475768196562 1107 88-89 1.3102377232075995 1107 90-91 1.3088782338389038 1107 92-93 1.4191129951161976 1107 94-95 1.4251914605874205 1107 96-97 1.5561919381611489 1107 98-99 1.345987183331136 1107 100-101 1.5339835392709489 1108 1 0.10806671522797373 1108 2 -0.07274270935647209 1108 3 -0.023445884740727507 1108 4 0.0344702930834373 1108 5 -0.03412232815288263 1108 6 0.13217576470545822 1108 7 0.2663772788368419 1108 8 0.16856044668029568 1108 9 0.29404110364922786 1108 10-11 0.15285567111697418 1108 12-13 0.18778525619872966 1108 14-15 0.19142162478912184 1108 16-17 0.21678299630575992 1108 18-19 0.3226759589221473 1108 20-21 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-2.3175999967597996 2316 76-77 -2.7758571772881204 2316 78-79 -2.4678685363573543 2316 80-81 -2.778417157202366 2316 82-83 -2.2262697638396993 2316 84-85 -1.974340439760077 2316 86-87 -2.6345044790650327 2316 88-89 -2.560946178994186 2316 90-91 -2.7430798305346045 2316 92-93 -2.434863925920144 2316 94-95 -2.8936723867907723 2316 96-97 -3.4126962261327733 2316 98-99 -3.83367890120331 2316 100-101 -4.266316553575454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1984.0 3 360.0 4 319.0 5 347.0 6 410.0 7 509.0 8 654.0 9 846.0 10 1114.0 11 1624.0 12 2551.0 13 3761.0 14 4193.0 15 5984.0 16 8243.0 17 11550.0 18 8657.0 19 4752.0 20 4402.0 21 4453.0 22 4685.0 23 5219.0 24 5628.0 25 6482.0 26 7443.0 27 8728.0 28 10277.0 29 12139.0 30 14960.0 31 18397.0 32 23325.0 33 30295.0 34 41665.0 35 60389.0 36 82951.0 37 112786.0 38 95869.0 39 12986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.34812760951901 19.39462390610256 15.175605279145824 19.08164320523261 2 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19.22775306299285 20-21 22.172527930853946 25.815861675617285 32.71037989510955 19.301230498419223 22-23 21.07716944523999 26.584351516080496 32.62374500320682 19.714734035472695 24-25 20.700158591616102 26.72865668222467 33.064496612786385 19.50668811337284 26-27 20.453953275257472 26.57886181747487 33.47189473098303 19.495290176284623 28-29 19.739018458961528 27.270094029520784 33.43332811574651 19.55755939577117 30-31 19.954634253801256 26.976641724382517 33.258288609098486 19.81043541271774 32-33 19.714526624951084 27.401006176649762 32.857114040077626 20.02735315832153 34-35 20.26527806021168 26.960736845842735 32.96019780056616 19.813787293379427 36-37 20.45868169952146 26.793326212733824 32.86506248810096 19.882929599643756 38-39 20.341641087281836 27.22625352430815 32.68331444290722 19.7487909455028 40-41 19.9890452420211 27.48982772473387 32.6276737514964 19.89345328174863 42-43 19.82840477755951 27.74348652126385 31.836359309262676 20.591749391913968 44-45 20.183976222823894 27.58207288698419 31.524120860857696 20.70983002933422 46-47 20.727962486041992 28.130950306269327 30.84122404456235 20.299863163126336 48-49 20.674504551029628 28.36808469433865 30.163724097863277 20.793686656768447 50-51 20.49953076192482 28.729151586896194 30.14235303326305 20.628964617915937 52-53 20.62228765085645 29.29954503962441 29.32698632536164 20.7511809841575 54-55 20.079802148767666 28.93114341390203 29.515579170751582 21.473475266578713 56-57 20.886793761726608 29.001727834592504 28.947117936407295 21.16436046727359 58-59 21.18873554372999 28.418221696283013 29.280536135330937 21.112506624656064 60-61 20.87923079405027 28.76359177878876 30.009598941696513 20.34757848546446 62-63 20.949276309314644 28.848161288396913 29.869685661227898 20.332876741060552 64-65 20.510417179210652 29.218919265550348 29.84921728987982 20.42144626535918 66-67 20.681764285495348 29.1054515653912 29.237897195733993 20.97488695337946 68-69 20.64427302115798 29.064958026029668 29.44348193602922 20.847287016783138 70-71 20.850935790591997 29.207992275220285 29.261071901920467 20.68000003226725 72-73 20.648813120170626 29.031951013125173 29.299439612996593 21.01979625370761 74-75 20.148917204131926 30.092730404198075 28.947455459961358 20.810896931708637 76-77 20.27298043847251 29.42207658102448 29.3078439724551 20.99709900804791 78-79 20.265585887048957 29.287939083526204 29.347877821779463 21.098597207645376 80-81 20.23987957619339 29.988496493285854 28.89493732766924 20.876686602851517 82-83 20.218195311019066 29.585428909063776 28.972626773088976 21.223749006828182 84-85 20.167207759472486 29.31015708495493 28.778125063015462 21.744510092557118 86-87 20.439278570045815 29.374475704975154 28.682164289862556 21.504081435116472 88-89 20.355253626987725 28.971618105174173 28.727130174698768 21.945998093139334 90-91 20.172918799313035 28.888071458476013 28.957687489361977 21.981322252848972 92-93 20.27358719613858 29.202073642207182 29.030990900592137 21.4933482610621 94-95 20.00546877394605 28.805113222979173 29.271733536812594 21.917684466262184 96-97 19.873347577899466 28.840003516778502 29.33428834841273 21.9523605569093 98-99 20.04928870191629 29.07767205669411 28.70530438395173 22.167734857437875 100-101 19.971511901766068 28.588355046232667 29.51599269569033 21.924140356310936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1573.0 1 996.0 2 610.5 3 799.5 4 698.0 5 708.5 6 1177.5 7 2357.0 8 3601.5 9 3908.5 10 3257.5 11 2554.0 12 2437.0 13 2492.5 14 2505.0 15 2434.5 16 2307.0 17 2282.5 18 2367.0 19 2486.0 20 2569.0 21 2756.0 22 3010.5 23 3198.0 24 3584.5 25 4204.0 26 4827.5 27 5575.0 28 6332.0 29 7214.0 30 8427.5 31 9464.5 32 10661.0 33 12186.0 34 13709.5 35 14814.0 36 16501.0 37 18266.5 38 18686.5 39 18482.5 40 18461.0 41 18985.5 42 19101.0 43 19541.5 44 20361.5 45 20962.5 46 20854.0 47 22341.5 48 28378.5 49 28010.0 50 21831.5 51 18243.5 52 15630.5 53 14694.5 54 14265.5 55 13238.0 56 12026.5 57 10872.5 58 9955.5 59 9314.5 60 8535.0 61 7799.0 62 6589.5 63 4571.0 64 2966.0 65 2230.0 66 1671.5 67 1202.0 68 813.0 69 511.0 70 363.5 71 299.0 72 270.5 73 244.0 74 183.5 75 126.5 76 86.0 77 53.5 78 30.5 79 21.0 80 15.5 81 9.0 82 6.5 83 4.5 84 1.5 85 1.0 86 0.5 87 0.5 88 1.5 89 2.5 90 2.5 91 2.5 92 3.0 93 3.5 94 2.0 95 0.0 96 1.0 97 2.0 98 3.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3299851675773871 2 0.2877908708935045 3 0.28263736900845016 4 0.2876298239595965 5 0.2876298239595965 6 0.20630112233608242 7 0.19341736762344652 8 0.19953715111194853 9 0.19357841455735444 10-11 0.2116961946219987 12-13 0.2019528551205678 14-15 0.19607464203292763 16-17 0.19792668177286907 18-19 0.19325632068953855 20-21 0.18480135665937125 22-23 0.18753915453580638 24-25 0.17811790890219137 26-27 0.17964785477431688 28-29 0.1993761041780406 30-31 0.2104078191507351 32-33 0.20364384792660126 34-35 0.21306509356021625 36-37 0.18343245772115369 38-39 0.18270774651856792 40-41 0.1795673313073629 42-43 0.18753915453580638 44-45 0.1905185228131034 46-47 0.1966383063016055 48-49 0.2088778732786096 50-51 0.21185724155590663 52-53 0.23069973282313663 54-55 0.20605955193522046 56-57 0.1748969702240324 58-59 0.176104822228342 60-61 0.17344754781886085 62-63 0.18166094144816625 64-65 0.17256178968236713 66-67 0.17070974994242571 68-69 0.16571729499127932 70-71 0.17932576090650099 72-73 0.1767490099639738 74-75 0.18190251184902814 76-77 0.18504292706023315 78-79 0.18319088732029173 80-81 0.18133884758035035 82-83 0.17385016515363072 84-85 0.16901875713639228 86-87 0.17022660914070187 88-89 0.1723202192815052 90-91 0.17852052623696124 92-93 0.17095132034328764 94-95 0.16974346833897805 96-97 0.16934085100420815 98-99 0.18029204250994868 100-101 0.16571729499127932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 620937.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.745491139296206 #Duplication Level Percentage of deduplicated Percentage of total 1 78.596036645965 42.24182591139511 2 11.979205071596839 12.876565200626402 3 3.8310013315581877 6.176971443596776 4 1.7785674277797234 3.8235991972150387 5 0.9610179886182756 2.582519189599389 6 0.5795880803189294 1.8690147621133635 7 0.3913939364055397 1.472496153874074 8 0.2911136655154392 1.251683754439046 9 0.22297481115739642 1.0785501663611483 >10 1.2187046398956054 12.16715371918311 >50 0.09676900641399797 3.5250170034291664 >100 0.04553834640121638 4.430584309231291 >500 0.005392698915932812 2.036400630863641 >1k 0.0020971606895294264 2.285979264360806 >5k 5.99188768436979E-4 2.181639293711669 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7930 1.2771021858900338 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5619 0.9049227216287642 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4351 0.7007152094334852 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2731 0.43981917650260816 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1939 0.3122700048475127 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1596 0.25703090651708627 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1319 0.21242090582458445 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1254 0.2019528551205678 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1007 0.16217426244530445 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 983 0.15830913603151367 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.1515451648073798 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 887 0.14284863037635057 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 835 0.13447418981313725 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 825 0.13286372047405776 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 742 0.119496824959698 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 727 0.11708112095107877 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 0.11675902708326287 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 708 0.11402122920682774 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 687 0.11063924359476082 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 642 0.10339213156890313 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 641 0.10323108463499517 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 8.052346695397439E-5 0.0 0.0 0.0 0.0 18-19 1.6104693390794878E-4 0.0 0.0 0.0 0.0 20-21 1.6104693390794878E-4 0.0 0.0 0.0 0.0 22-23 1.6104693390794878E-4 0.0 0.0 0.0 0.0 24-25 3.2209386781589756E-4 0.0 0.0 3.2209386781589756E-4 0.0 26-27 3.2209386781589756E-4 0.0 0.0 4.0261733476987195E-4 0.0 28-29 4.0261733476987195E-4 0.0 0.0 0.0010468050704016672 0.0 30-31 4.831408017238464E-4 0.0 0.0 0.0028988448103430785 0.0 32-33 4.831408017238464E-4 0.0 0.0 0.004831408017238463 0.0 34-35 5.636642686778207E-4 0.0 0.0 0.0066029242902259 0.0 36-37 6.441877356317951E-4 0.0 0.0 0.009340722166661031 0.0 38-39 6.441877356317951E-4 0.0 0.0 0.011112238439648467 0.0 40-41 6.441877356317951E-4 0.0 0.0 0.0166683576594727 0.0 42-43 6.441877356317951E-4 0.0 0.0 0.024479133954008216 0.0 44-45 6.441877356317951E-4 0.0 0.0 0.03704079479882822 0.0 46-47 9.662816034476928E-4 0.0 0.0 0.06739814184047657 0.0 48-49 9.662816034476928E-4 0.0 0.0 0.0896226187197735 0.0 50-51 9.662816034476928E-4 0.0 0.0 0.1176447852197566 0.0 52-53 9.662816034476928E-4 0.0 0.0 0.15420243921686097 0.0 54-55 9.662816034476928E-4 0.0 0.0 0.18649234946540472 0.0 56-57 9.662816034476928E-4 0.0 0.0 0.23222967869526218 0.0 58-59 9.662816034476928E-4 0.0 0.0 0.30896854270239976 0.0 60-61 9.662816034476928E-4 0.0 0.0 0.4106696814652694 0.0 62-63 9.662816034476928E-4 0.0 0.0 0.5365278603143314 0.0 64-65 9.662816034476928E-4 0.0 0.0 0.6412083673544982 0.0 66-67 9.662816034476928E-4 0.0 0.0 0.7999201207207817 0.0 68-69 9.662816034476928E-4 0.0 0.0 0.9867345640540022 0.0 70-71 0.0010468050704016672 0.0 0.0 1.1302273821659847 0.0 72-73 0.0011273285373556415 0.0 0.0 1.3718783064948616 0.0 74-75 0.0012883754712635902 0.0 0.0 1.5738311616154297 0.0 76-77 0.0014494224051715392 0.0 0.0 1.7611287457503741 0.0 78-79 0.0014494224051715392 0.0 0.0 1.9951299407186236 0.0 80-81 0.001610469339079488 0.0 0.0 2.243061695469911 0.0 82-83 0.001610469339079488 0.0 0.0 2.5493729637628295 0.0 84-85 0.0016909928060334623 0.0 0.0 2.9468367966476468 0.0 86-87 0.0017715162729874368 0.0 0.0 3.4489811365726313 0.0 88-89 0.0017715162729874368 0.0 0.0 4.029555333310787 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 45 9.543204E-4 42.292496 1 GTACACG 70 2.0322911E-4 33.98504 1 CGATCCG 30 0.0039532552 31.663092 70-71 TCGACCG 40 4.4968157E-4 29.684147 18-19 GTTCGAC 50 4.9558716E-5 28.49793 16-17 CGACCGT 45 8.9538493E-4 26.385908 18-19 GTATAGA 170 1.1031807E-7 25.188913 1 GTACATG 7540 0.0 24.925367 1 CATATAG 115 1.274801E-4 24.799812 3 TACATGG 7615 0.0 24.531134 2 CTTACTA 315 0.0 24.126205 9 CCAAGAC 260 2.3646862E-11 23.766487 3 TAATACC 140 1.7693474E-5 23.764568 4 GAGTACT 5220 0.0 23.385262 12-13 ACATGGG 7675 0.0 23.348444 3 TAGACTG 185 2.5227564E-7 23.122282 5 GATAGTC 145 2.3326818E-5 22.930294 7 CATGGGG 1685 0.0 22.565763 4 GTATCAA 11475 0.0 22.390144 1 ATGGGAG 1725 0.0 22.31803 5 >>END_MODULE