FastQCFastQC Report
Thu 26 May 2016
SRR937270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620937
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94151.5162568827433378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74741.2036647840280092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60900.9807758274994081No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29260.4712233286146582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17220.27732282018948784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.2708809428331699No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.26782105108891885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.21193776502286063No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12870.20726740393953008No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12160.19583307163206573No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10520.16942137447116212No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.1575039013619739No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9510.1531556341464593No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8530.13737303462348033No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.13672884688784853No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8430.13576256528440084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.1346352367470452No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8260.1330247674079657No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8030.12932068792808288No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7500.1207852004309616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7270.11708112095107877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG308.293001E-539.58182518-19
GTACACG550.002573091534.5608371
CGACCGT352.0538874E-433.92727718-19
GCGGTAT902.4038625E-531.6807651
CGGTATC908.936477E-426.3921322
CATATAG1109.461834E-525.9122773
GGATATG2850.025.0111331
GAGTACT63450.023.9943212-13
TCAATAC1801.9457184E-723.752923
GTACATG66050.023.3109051
GTATCAA118350.023.2486231
AGTACTT64900.022.68971412-13
CAATACA2103.4353434E-822.621834
TACATGG67450.022.537982
GTACTTT67650.022.50283814-15
GATATGA2405.9062586E-921.773512
GTTCGAC550.002878131621.59008616-17
GGTATCA89200.021.4697571
GTAAGGT2005.371512E-721.3776283
CTTACAC2901.05501385E-1021.2957213
Produced by FastQC (version 0.11.5)