##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937270_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 620937 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16582841737568 33.0 31.0 34.0 31.0 34.0 2 32.352117847704356 34.0 31.0 34.0 31.0 34.0 3 32.4080993724001 34.0 31.0 34.0 31.0 34.0 4 36.054751126120685 37.0 35.0 37.0 35.0 37.0 5 35.935822796837684 37.0 35.0 37.0 35.0 37.0 6 36.057719221112606 37.0 35.0 37.0 35.0 37.0 7 36.017575051897374 37.0 35.0 37.0 35.0 37.0 8 36.008337399768415 37.0 35.0 37.0 35.0 37.0 9 37.67515867149163 39.0 37.0 39.0 35.0 39.0 10-11 37.6094644062119 39.0 37.0 39.0 35.0 39.0 12-13 37.52245074782144 39.0 37.0 39.0 35.0 39.0 14-15 38.60495106588913 40.0 38.0 41.0 34.0 41.0 16-17 38.63275662426301 40.0 38.0 41.0 34.5 41.0 18-19 38.765428698885714 40.0 38.0 41.0 35.0 41.0 20-21 38.73512288686292 40.0 38.0 41.0 34.5 41.0 22-23 38.60519344152466 40.0 38.0 41.0 34.0 41.0 24-25 38.44975013568204 40.0 38.0 41.0 34.0 41.0 26-27 38.204764734586604 40.0 37.5 41.0 34.0 41.0 28-29 38.141825177111365 40.0 37.0 41.0 34.0 41.0 30-31 38.08440469806115 40.0 37.0 41.0 34.0 41.0 32-33 37.87544630131559 40.0 36.5 41.0 33.5 41.0 34-35 37.67625459587688 40.0 36.0 41.0 33.0 41.0 36-37 37.45469347131835 40.0 35.5 41.0 33.0 41.0 38-39 37.26161993889879 40.0 35.0 41.0 33.0 41.0 40-41 37.026519598606626 40.0 35.0 41.0 33.0 41.0 42-43 36.692365731145024 39.5 35.0 41.0 31.5 41.0 44-45 36.18294206980741 39.0 35.0 41.0 30.5 41.0 46-47 35.54037929773874 39.0 35.0 41.0 28.0 41.0 48-49 34.99073658036161 39.0 35.0 41.0 24.0 41.0 50-51 34.70983610253536 39.0 35.0 41.0 23.0 41.0 52-53 34.46236011060704 39.0 35.0 40.0 22.5 41.0 54-55 34.257293413019354 38.0 34.5 40.0 21.5 41.0 56-57 34.021009377762965 38.0 34.0 40.0 20.0 41.0 58-59 33.77248174935622 38.0 34.0 40.0 18.5 41.0 60-61 33.43232566266787 37.5 33.5 40.0 16.0 41.0 62-63 33.07974077885518 37.0 33.0 40.0 12.0 41.0 64-65 32.74047367124201 36.5 33.0 39.5 8.0 41.0 66-67 32.347127808457216 36.0 32.5 39.0 6.0 40.5 68-69 31.911034452770572 35.0 32.0 39.0 2.0 40.0 70-71 31.523764890802127 35.0 31.5 38.0 2.0 40.0 72-73 31.09469962331122 35.0 31.0 37.5 2.0 39.5 74-75 30.672792086797855 35.0 31.0 37.0 2.0 39.0 76-77 29.358258567294268 33.5 29.5 35.5 2.0 38.0 78-79 29.93026184620984 34.0 30.5 36.0 2.0 38.5 80-81 29.735564155461827 34.0 30.0 36.0 2.0 37.0 82-83 29.39236428172262 34.0 30.0 35.0 2.0 37.0 84-85 29.05068469103951 34.0 30.0 35.0 2.0 36.5 86-87 28.72210143702179 34.0 29.0 35.0 2.0 36.0 88-89 28.341829364331645 34.0 29.0 35.0 2.0 36.0 90-91 27.883629096027455 34.0 29.0 35.0 2.0 35.0 92-93 27.49950880685158 34.0 27.0 35.0 2.0 35.0 94-95 27.114820021999012 34.0 27.0 35.0 2.0 35.0 96-97 26.72026550197524 33.0 25.5 35.0 2.0 35.0 98-99 26.14701008314853 33.0 25.0 35.0 2.0 35.0 100-101 24.686280572747314 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.035922686119626235 1101 2 0.1394726169212177 1101 3 0.1561306455917446 1101 4 0.1696322076397152 1101 5 -0.1373164824295543 1101 6 0.09780049636306387 1101 7 0.05067198010953433 1101 8 0.07972873493734767 1101 9 0.26865290606418313 1101 10-11 0.20808993394172148 1101 12-13 0.21042808072689212 1101 14-15 0.15396930898705108 1101 16-17 0.13339836626016677 1101 18-19 0.25232205151500864 1101 20-21 0.015106650840664315 1101 22-23 0.24004611360551564 1101 24-25 0.20183027454057623 1101 26-27 0.08486661018701369 1101 28-29 0.18091314956836868 1101 30-31 0.24826294471187538 1101 32-33 0.22962285894146817 1101 34-35 0.269493313427958 1101 36-37 0.47518119510799295 1101 38-39 0.4990998218119671 1101 40-41 0.46906611677280097 1101 42-43 0.33087501158425425 1101 44-45 0.42245848627567284 1101 46-47 0.39028390195586127 1101 48-49 0.4139728497757673 1101 50-51 0.5135439060654221 1101 52-53 0.8359424459228606 1101 54-55 0.9132194348553355 1101 56-57 0.8692134054029523 1101 58-59 0.7764151512039987 1101 60-61 0.4373993259814739 1101 62-63 0.41478647413398306 1101 64-65 0.37739926473446417 1101 66-67 0.05028760307728675 1101 68-69 0.21502950887511574 1101 70-71 0.3300762393592116 1101 72-73 0.34255689416461976 1101 74-75 0.4870589656084299 1101 76-77 0.6026227824257262 1101 78-79 0.5914824998808257 1101 80-81 0.42350814443890883 1101 82-83 0.15766006398218124 1101 84-85 0.2584884941730614 1101 86-87 0.013270333237386467 1101 88-89 0.07915792710103986 1101 90-91 0.01908031435404567 1101 92-93 -0.24782397661404332 1101 94-95 -0.306638692045734 1101 96-97 -0.13944420887680664 1101 98-99 -0.27272944693843826 1101 100-101 -0.6005774585324382 1102 1 0.12262099962360651 1102 2 0.15163061075208617 1102 3 0.07550131418125972 1102 4 0.10243275244602756 1102 5 0.004883974246325806 1102 6 0.0949462721111658 1102 7 0.12951867321532973 1102 8 0.16984210272166678 1102 9 0.27893812819293373 1102 10-11 0.17765989748777145 1102 12-13 0.08956420218665073 1102 14-15 0.2483940576150232 1102 16-17 0.28617447490096026 1102 18-19 0.24661861042218902 1102 20-21 0.25598815546751297 1102 22-23 0.27534335352059713 1102 24-25 0.20115427405985997 1102 26-27 0.3192535027880581 1102 28-29 0.2516378220836941 1102 30-31 0.24483286819863537 1102 32-33 0.014759861705563537 1102 34-35 0.20352067392149564 1102 36-37 0.09479822831714557 1102 38-39 -0.07996218996546389 1102 40-41 0.07668539330206414 1102 42-43 0.08943267692895773 1102 44-45 -0.04075206534071185 1102 46-47 0.13706914411425686 1102 48-49 0.30212731838931717 1102 50-51 0.465277471742624 1102 52-53 0.2561543595550404 1102 54-55 0.4466288808353909 1102 56-57 0.35977443572782875 1102 58-59 0.22115837116954395 1102 60-61 0.419527876235847 1102 62-63 0.31454311396093004 1102 64-65 0.5541658793240529 1102 66-67 -0.025424450762400852 1102 68-69 0.21644660617912237 1102 70-71 0.23583175752609975 1102 72-73 0.1672173917009694 1102 74-75 0.41496226248781554 1102 76-77 0.17452920392954852 1102 78-79 0.24902065635240334 1102 80-81 0.308953603718642 1102 82-83 0.4072294266389136 1102 84-85 0.4578685766951942 1102 86-87 0.24116762122360313 1102 88-89 0.19925567176314019 1102 90-91 0.27605063042182465 1102 92-93 0.21138565363873596 1102 94-95 0.380052611082899 1102 96-97 0.6084326488726646 1102 98-99 0.8126619323028379 1102 100-101 0.4500374515344632 1103 1 0.15818199913365305 1103 2 0.07583933196717396 1103 3 0.2151485459304041 1103 4 0.2032509788056558 1103 5 0.13956305312922979 1103 6 0.06378017705972638 1103 7 0.15697580207373107 1103 8 -3.244822856771634E-4 1103 9 0.17933989203418577 1103 10-11 0.2278093340384828 1103 12-13 0.2522810076741564 1103 14-15 0.2498957724606825 1103 16-17 0.21662768411212596 1103 18-19 0.22561910037809696 1103 20-21 0.19263930686388875 1103 22-23 0.28517431873372345 1103 24-25 0.20816554304565926 1103 26-27 0.40511092562000073 1103 28-29 0.36133404942322755 1103 30-31 0.41733164330887007 1103 32-33 0.4357912188834163 1103 34-35 0.4096916685319769 1103 36-37 0.4375517804974436 1103 38-39 0.308736977109497 1103 40-41 0.5809529092256369 1103 42-43 0.6058290512552702 1103 44-45 0.7285252497009296 1103 46-47 0.5099500848295904 1103 48-49 0.043462448227629125 1103 50-51 0.1049590004050458 1103 52-53 0.15699953348938323 1103 54-55 0.18827023311368407 1103 56-57 0.056903294130570714 1103 58-59 -0.09381223147621398 1103 60-61 -0.0041149459101603725 1103 62-63 0.17139024771889666 1103 64-65 0.4098738656052916 1103 66-67 0.2782992141077614 1103 68-69 0.37551813974110715 1103 70-71 0.46615267864319776 1103 72-73 0.2770924529454568 1103 74-75 0.28741939134621575 1103 76-77 0.04643023283694703 1103 78-79 0.36762183224657363 1103 80-81 0.5396934371336357 1103 82-83 0.3130035569671854 1103 84-85 0.22542124695486976 1103 86-87 0.251025534011454 1103 88-89 0.07802343266466139 1103 90-91 0.28575665687944607 1103 92-93 0.3422969716201152 1103 94-95 0.452517089279862 1103 96-97 0.2860686116360185 1103 98-99 -0.004606563532533414 1103 100-101 0.1776905627544494 1104 1 0.15767693687367768 1104 2 0.19944226458675018 1104 3 0.20659972712439867 1104 4 0.1792826324772463 1104 5 0.09461483946694216 1104 6 0.22673607139231677 1104 7 0.1117738935435284 1104 8 0.22821968156861772 1104 9 0.11841122916906244 1104 10-11 0.11736594988858684 1104 12-13 0.23388929348819687 1104 14-15 0.2990206415401673 1104 16-17 0.24599577441819065 1104 18-19 0.3215436901604747 1104 20-21 0.1247247252276722 1104 22-23 0.0996023415429903 1104 24-25 0.05983201575599395 1104 26-27 0.175851062941085 1104 28-29 0.12410956437726384 1104 30-31 0.05638552437486055 1104 32-33 0.1195338000541355 1104 34-35 0.20884097610791486 1104 36-37 0.5247297160263642 1104 38-39 0.4079072319681032 1104 40-41 0.5239786278338059 1104 42-43 0.6370672684087708 1104 44-45 0.6230836111579237 1104 46-47 0.6878390642314329 1104 48-49 0.8903296657302491 1104 50-51 1.0064113453369643 1104 52-53 0.8867504935731745 1104 54-55 1.06213617050458 1104 56-57 1.0469160952477594 1104 58-59 0.8590610374636256 1104 60-61 0.8774677376694981 1104 62-63 1.0311481902302404 1104 64-65 1.04972607088866 1104 66-67 0.8707640110354973 1104 68-69 0.851612635260711 1104 70-71 0.871327821201092 1104 72-73 0.8470633594973052 1104 74-75 0.9352280728311477 1104 76-77 0.9106918411227625 1104 78-79 1.0971842667863108 1104 80-81 1.0199541144291082 1104 82-83 1.0734620697101143 1104 84-85 1.131641426170514 1104 86-87 1.0382348904643806 1104 88-89 1.3074985868599356 1104 90-91 1.359580699704999 1104 92-93 1.2463910016445539 1104 94-95 1.0830070229467985 1104 96-97 1.2305129267762425 1104 98-99 1.3730450133323515 1104 100-101 1.4594350059058527 1105 1 -0.0030714796596242877 1105 2 0.05410049193600486 1105 3 -0.024777981955601547 1105 4 0.028725764511989382 1105 5 -0.18843872801292605 1105 6 -0.12984333401675485 1105 7 -0.10351846487495209 1105 8 -0.0607408242649754 1105 9 -0.14998884001524004 1105 10-11 -0.0747332463912187 1105 12-13 -0.09200709206980662 1105 14-15 0.0738443419808874 1105 16-17 -0.07479388997225556 1105 18-19 -0.07320311937031931 1105 20-21 -0.011946020977589455 1105 22-23 -0.028164209597562717 1105 24-25 -0.10988299307813776 1105 26-27 -0.15260141992157372 1105 28-29 -0.2533904301492953 1105 30-31 -0.07102525305091945 1105 32-33 0.12051792488364299 1105 34-35 0.11739399514892312 1105 36-37 -0.0992711071792769 1105 38-39 0.029906964864146346 1105 40-41 0.04193699026760811 1105 42-43 -0.14182429395233953 1105 44-45 0.045973763560439806 1105 46-47 0.11605049671872081 1105 48-49 0.2671104500087722 1105 50-51 0.25207911617928147 1105 52-53 0.41045049641935805 1105 54-55 0.27972409039966806 1105 56-57 0.3349373584743276 1105 58-59 0.4007040771066599 1105 60-61 0.5782784638638603 1105 62-63 0.41350312760311425 1105 64-65 0.5785535939811126 1105 66-67 0.3008473606067028 1105 68-69 0.23141038064058606 1105 70-71 0.20285199958275157 1105 72-73 0.23279322106301592 1105 74-75 0.1571422010979724 1105 76-77 0.2347414874289555 1105 78-79 0.21216485796181317 1105 80-81 0.27322121181915193 1105 82-83 0.10246364825071907 1105 84-85 0.02222075714077576 1105 86-87 0.11284118579816038 1105 88-89 0.33003055723328956 1105 90-91 0.18283669695513893 1105 92-93 -0.017740877650730624 1105 94-95 0.0913682525883317 1105 96-97 -0.06232168113922398 1105 98-99 -0.1303485205624817 1105 100-101 0.08751244853871043 1106 1 -0.034112058359809794 1106 2 0.1695605000162601 1106 3 0.01821410666213552 1106 4 -5.576793013801762E-4 1106 5 -0.024018358074044954 1106 6 -0.02064659262553903 1106 7 0.006886039546742495 1106 8 -0.0118958946251837 1106 9 -0.0832348153854241 1106 10-11 -0.10542325316752255 1106 12-13 -0.020276656227672163 1106 14-15 -7.109767723605387E-4 1106 16-17 0.214698357899195 1106 18-19 0.007003034021998644 1106 20-21 0.020882688297817253 1106 22-23 0.010848673920634155 1106 24-25 0.06444210252752924 1106 26-27 0.14875255725277015 1106 28-29 -0.09807851733054918 1106 30-31 0.09534891407355417 1106 32-33 0.12784893959697285 1106 34-35 0.029863239944319275 1106 36-37 0.027636998086357778 1106 38-39 0.030153861554445882 1106 40-41 0.09480815294794098 1106 42-43 0.028282181581374743 1106 44-45 0.15865591667059675 1106 46-47 0.4629895121673897 1106 48-49 0.5555531197422354 1106 50-51 0.6448703741125783 1106 52-53 0.5365302686331148 1106 54-55 0.6118482356133939 1106 56-57 0.6237434259337746 1106 58-59 0.5135369333914142 1106 60-61 0.4940193570099609 1106 62-63 0.4935468208355829 1106 64-65 0.24503464278969744 1106 66-67 0.5265494129488033 1106 68-69 0.9846034709534628 1106 70-71 0.8354504857105809 1106 72-73 0.8997285170290859 1106 74-75 0.9919234249787579 1106 76-77 0.6466688420858944 1106 78-79 0.8143790189299018 1106 80-81 0.8158734166814128 1106 82-83 0.7653203391510282 1106 84-85 0.46193414321665927 1106 86-87 0.8585621775746866 1106 88-89 0.8318764039179278 1106 90-91 0.7167406359727302 1106 92-93 0.8443603735062624 1106 94-95 0.782093273804346 1106 96-97 0.996018617317187 1106 98-99 0.606303560317027 1106 100-101 0.6536262518806879 1107 1 0.04551205280091608 1107 2 0.0949538221281756 1107 3 0.042219207826647676 1107 4 -0.011605908493095285 1107 5 -0.11400581985824942 1107 6 0.06749663502036896 1107 7 -0.018153893811778232 1107 8 0.007282852240258819 1107 9 -0.0460044729031992 1107 10-11 0.0270803537164781 1107 12-13 0.03327161590313921 1107 14-15 0.14642305331936 1107 16-17 0.27128779144665316 1107 18-19 0.22620496799883938 1107 20-21 0.011431793447073346 1107 22-23 -0.11654303368129604 1107 24-25 0.03687305646246841 1107 26-27 0.032879261934859016 1107 28-29 0.0025728687434423136 1107 30-31 0.009529764954663733 1107 32-33 -0.02645162548152058 1107 34-35 -0.013863787639756708 1107 36-37 0.11213362307347552 1107 38-39 0.10735728122399024 1107 40-41 0.1408656999233031 1107 42-43 0.2870052587686871 1107 44-45 0.4785274858368922 1107 46-47 0.35703192740562173 1107 48-49 0.45692845896108025 1107 50-51 0.511034581800395 1107 52-53 0.5840455978900962 1107 54-55 0.7527872277469498 1107 56-57 0.615986478388173 1107 58-59 0.7201770350711953 1107 60-61 0.4871104565943085 1107 62-63 0.5513321081840061 1107 64-65 0.6385746706142328 1107 66-67 0.6092361550737664 1107 68-69 0.5601827193475479 1107 70-71 0.43175643535160546 1107 72-73 0.5013299305840988 1107 74-75 0.4773187652281514 1107 76-77 0.5967768339102903 1107 78-79 0.8235744261642814 1107 80-81 0.9007779630728123 1107 82-83 0.652220909382212 1107 84-85 0.44363457432517706 1107 86-87 0.6836529937610081 1107 88-89 1.0293919478703835 1107 90-91 0.8810674797774745 1107 92-93 0.7027665601710993 1107 94-95 0.3767568030544588 1107 96-97 0.7939375740689307 1107 98-99 0.7260821663422412 1107 100-101 1.1201927988904075 1108 1 0.03848616488194523 1108 2 0.036072966527456174 1108 3 -0.03130064957323242 1108 4 -0.0867291842977167 1108 5 -0.12276006787584492 1108 6 0.004163690394527464 1108 7 0.02202319021567689 1108 8 0.011560022893952748 1108 9 -0.09496912273328206 1108 10-11 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-2.4357911204205145 2316 100-101 -2.3865855413695627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 9.0 5 15.0 6 43.0 7 92.0 8 167.0 9 402.0 10 711.0 11 1325.0 12 2203.0 13 3073.0 14 4075.0 15 5757.0 16 8618.0 17 12502.0 18 10244.0 19 4830.0 20 3618.0 21 3534.0 22 3781.0 23 4035.0 24 4394.0 25 5033.0 26 5761.0 27 7143.0 28 8765.0 29 10598.0 30 13106.0 31 16583.0 32 20849.0 33 26899.0 34 38105.0 35 56748.0 36 82406.0 37 125867.0 38 116317.0 39 13326.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.534156256596084 19.784478105418177 15.991576342218346 18.689789295767387 2 15.302679659933293 22.686198438811022 39.84591029363688 22.165211607618808 3 19.637418932999644 28.73270557238496 30.764634737501552 20.865240757113845 4 11.98736747850426 17.888771324627136 37.82122824054614 32.30263295632246 5 11.854677121117433 40.25661510311636 32.5771681495751 15.311539626191106 6 28.208819896382405 38.009653153218444 17.683436483894504 16.09809046650465 7 26.30411780905309 36.47616424854696 20.164847641548175 17.054870300851775 8 27.867239349563643 32.603790722730324 19.3103326102326 20.21863731747343 9 28.420274520603538 16.44466346827456 20.618355807432962 34.51670620368894 10-11 24.78697516817326 28.4021567405389 25.594464494787715 21.21640359650013 12-13 27.506770375750822 26.383971100179014 27.11653260128184 18.992725922788324 14-15 23.64692392303889 26.081470422925353 28.88127136891504 21.390334285120712 16-17 19.64998059384446 29.945388984711812 29.01236357311611 21.39226684832761 18-19 19.55689546604567 26.74941258130857 34.65488447298196 19.0388074796638 20-21 21.505160748997078 24.78141904895344 34.70464797555952 19.008772226489967 22-23 20.53315188507561 25.528078849467732 34.55792118274202 19.38084808271464 24-25 20.422169452382736 25.700575985750536 34.928265496174724 18.94898906569201 26-27 20.070394465072123 25.59429049513673 35.309886227019334 19.025428812771818 28-29 19.427965879909713 26.173310925885513 35.32047777145208 19.078245422752683 30-31 19.609669917672676 25.864581857067392 35.114488449695294 19.41125977556463 32-33 19.508581385873285 26.12946240922992 34.79451216468015 19.567444040216643 34-35 20.04309615951377 25.79955776511949 34.83847797763702 19.318868097729723 36-37 20.29927399925274 25.54909040545241 34.71734607109268 19.43428952420217 38-39 20.04617219313086 25.933328126144943 34.69267791473041 19.327821765993786 40-41 19.69161122625967 26.339870228380658 34.467184271512245 19.501334273847426 42-43 19.691476339271333 26.540604967303416 33.65549399220436 20.11242470122089 44-45 20.022723722374412 26.3508214198864 33.25699708666097 20.369457771078224 46-47 20.51504419933101 27.07698204487734 32.40183786760976 20.00613588818189 48-49 20.46785744769598 27.29439540565307 31.659250455360205 20.57849669129074 50-51 20.374128132161555 27.627440464974708 31.457539170640498 20.540892232223236 52-53 20.570621955707228 28.298223731412147 30.482505054864706 20.648649258015915 54-55 20.04051940857124 27.944058737037736 30.763346362030287 21.25207549236074 56-57 20.765552705024824 28.03867381070865 30.096451008717473 21.099322475549048 58-59 21.10439545396715 27.445457429658727 30.461463884419835 20.98868323195429 60-61 20.723358408340943 27.84855790523032 31.233120268239773 20.194963418188962 62-63 20.977490470047684 27.7670681566729 31.100417594699625 20.155023778579793 64-65 20.55087714212553 28.157284877531858 31.015545860530136 20.276292119812478 66-67 20.763378571417068 28.09367133863822 30.390522710033384 20.752427379911328 68-69 20.72512992461393 28.04503516459802 30.558736232500237 20.671098678287812 70-71 20.89390711134946 28.363263906000125 30.274810488020528 20.468018494629888 72-73 20.752185809510465 28.02579005599602 30.3502609765564 20.871763157937117 74-75 20.317198041025094 29.046586046571555 29.856491077194626 20.779724835208725 76-77 20.309145694329697 28.33556383336796 30.43456904645721 20.920721425845134 78-79 20.15582901324933 28.289182316402467 30.497055256813493 21.05793341353471 80-81 20.136342334246468 28.970169276432227 30.077447470516333 20.81604091880497 82-83 20.138098746643156 28.48723492489119 30.195311084538172 21.179355243927482 84-85 19.993010563068395 28.343455133129446 29.88668737730237 21.77684692649979 86-87 20.076271827898804 28.292000637745858 29.77025044408692 21.861477090268416 88-89 20.046317098191928 27.9423677442317 29.868166979902956 22.143148177673417 90-91 19.92637963690714 27.639767558722344 30.18716741183595 22.246685392534562 92-93 19.98308960896734 27.84617986214005 30.25768214906912 21.91304837982349 94-95 19.578314708255427 27.539267268660105 30.38295350413971 22.499464518944755 96-97 19.464132432114692 27.47895519191158 30.502691899500274 22.554220476473457 98-99 19.558176726088575 27.707443870213407 29.822829414729913 22.91154998896811 100-101 19.197180693938428 27.478167451059974 30.496793168208068 22.827858686793522 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1135.0 1 843.5 2 868.5 3 1117.5 4 824.0 5 698.5 6 1305.5 7 2926.0 8 4308.5 9 4542.0 10 3749.0 11 2749.0 12 2469.0 13 2480.0 14 2528.0 15 2488.5 16 2427.5 17 2437.0 18 2513.0 19 2592.0 20 2670.5 21 2776.0 22 2992.0 23 3294.0 24 3620.0 25 4123.0 26 4746.5 27 5451.5 28 6179.5 29 7194.0 30 8289.5 31 9011.5 32 10247.5 33 11899.0 34 13386.5 35 14414.5 36 16248.5 37 18164.5 38 18587.5 39 18474.0 40 18479.5 41 18891.5 42 19117.0 43 19722.5 44 20467.0 45 21005.0 46 20853.5 47 22470.0 48 28835.5 49 27961.0 50 21568.0 51 18251.5 52 15617.5 53 14735.0 54 14047.0 55 12999.5 56 11923.5 57 10737.0 58 9917.0 59 9334.5 60 8429.0 61 7630.0 62 6433.0 63 4498.5 64 2966.5 65 2202.0 66 1564.0 67 1142.5 68 790.5 69 458.0 70 338.0 71 277.5 72 249.5 73 257.0 74 204.0 75 145.0 76 98.0 77 46.5 78 23.5 79 18.0 80 13.5 81 6.0 82 3.5 83 3.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04895826790801643 2 0.0 3 0.0 4 0.0 5 0.013688989382175647 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0053950722859162845 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011273285373556415 24-25 2.415704008619232E-4 26-27 0.001207852004309616 28-29 0.00201308667384936 30-31 1.610469339079488E-4 32-33 0.0 34-35 0.0 36-37 1.610469339079488E-4 38-39 8.05234669539744E-5 40-41 0.0 42-43 0.012400613910912055 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.05234669539744E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.247112025857696E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007891299761489492 92-93 0.0027377978764351295 94-95 0.0 96-97 0.0 98-99 0.0016104693390794878 100-101 0.01207852004309616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 620937.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.435764039078556 #Duplication Level Percentage of deduplicated Percentage of total 1 77.60733459335243 39.91792549845495 2 12.443011248839158 12.800315810617821 3 4.114890698244082 6.349576410044455 4 1.8050723619436488 3.71381104329583 5 0.9779981046594836 2.5152039870965623 6 0.6297170532747854 1.9433986658175435 7 0.4234379419440236 1.5245897846918088 8 0.29918796246785506 1.2311169152663428 9 0.23126871885856565 1.0705934927546261 >10 1.2954970860408799 12.298648153506788 >50 0.11085702746286275 3.920783813934821 >100 0.053538900484958955 5.088076803727132 >500 0.004724020631025601 1.8197852535201946 >1k 0.002519477669880321 2.0838274435894415 >5k 9.448041262051203E-4 3.7223469236817297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9415 1.5162568827433378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7474 1.2036647840280092 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6090 0.9807758274994081 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2926 0.4712233286146582 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1722 0.27732282018948784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1682 0.2708809428331699 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 0.26782105108891885 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1316 0.21193776502286063 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1287 0.20726740393953008 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1216 0.19583307163206573 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1052 0.16942137447116212 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 978 0.1575039013619739 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 951 0.1531556341464593 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 853 0.13737303462348033 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.13672884688784853 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 843 0.13576256528440084 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.1346352367470452 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 826 0.1330247674079657 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 803 0.12932068792808288 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 750 0.1207852004309616 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 727 0.11708112095107877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 3.2209386781589756E-4 0.0 26-27 0.0 0.0 0.0 3.2209386781589756E-4 0.0 28-29 3.2209386781589756E-4 0.0 0.0 8.857581364937183E-4 0.0 30-31 3.2209386781589756E-4 0.0 0.0 0.0027377978764351295 0.0 32-33 3.2209386781589756E-4 0.0 0.0 0.004831408017238463 0.0 34-35 4.0261733476987195E-4 0.0 0.0 0.0066029242902259 0.0 36-37 8.05234669539744E-4 0.0 0.0 0.009179675232753082 0.0 38-39 8.05234669539744E-4 0.0 0.0 0.010870668038786543 0.0 40-41 8.857581364937184E-4 0.0 0.0 0.016426787258610777 0.0 42-43 0.0010468050704016672 0.0 0.0 0.024318087020100267 0.0 44-45 0.0012883754712635902 0.0 0.0 0.03687974786492027 0.0 46-47 0.0012883754712635902 0.0 0.0 0.06691500103875272 0.0 48-49 0.0012883754712635902 0.0 0.0 0.08881738405023376 0.0 50-51 0.0012883754712635902 0.0 0.0 0.11667850361630891 0.0 52-53 0.0013688989382175647 0.0 0.0 0.15259196987778148 0.0 54-55 0.0014494224051715392 0.0 0.0 0.18552606786195702 0.0 56-57 0.0014494224051715392 0.0 0.0 0.23190758482744628 0.0 58-59 0.0014494224051715392 0.0 0.0 0.30896854270239976 0.0 60-61 0.0014494224051715392 0.0 0.0 0.4115554396017631 0.0 62-63 0.0014494224051715392 0.0 0.0 0.5382188531203649 0.0 64-65 0.0014494224051715392 0.0 0.0 0.6441877356317951 0.0 66-67 0.001610469339079488 0.0 0.0 0.8048320522049741 0.0 68-69 0.0017715162729874368 0.0 0.0 0.993901152612906 0.0 70-71 0.0017715162729874368 0.0 0.0 1.1395681043326458 0.0 72-73 0.0017715162729874368 0.0 0.0 1.3844399673396817 0.0 74-75 0.0017715162729874368 0.0 0.0 1.588325385667145 0.0 76-77 0.0017715162729874368 0.0 0.0 1.7770723922072609 0.0 78-79 0.0017715162729874368 0.0 0.0 2.0120398687789582 0.0 80-81 0.002415704008619232 0.0 0.0 2.2627094214066803 0.0 82-83 0.002657274409481155 0.0 0.0 2.5736105273159757 0.0 84-85 0.0027377978764351295 0.0 0.0 2.9789656599622827 0.0 86-87 0.0027377978764351295 0.0 0.0 3.4890012996487565 0.0 88-89 0.0027377978764351295 0.0 0.0 4.078996742020527 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 30 8.293001E-5 39.581825 18-19 GTACACG 55 0.0025730915 34.560837 1 CGACCGT 35 2.0538874E-4 33.927277 18-19 GCGGTAT 90 2.4038625E-5 31.680765 1 CGGTATC 90 8.936477E-4 26.392132 2 CATATAG 110 9.461834E-5 25.912277 3 GGATATG 285 0.0 25.011133 1 GAGTACT 6345 0.0 23.99432 12-13 TCAATAC 180 1.9457184E-7 23.75292 3 GTACATG 6605 0.0 23.310905 1 GTATCAA 11835 0.0 23.248623 1 AGTACTT 6490 0.0 22.689714 12-13 CAATACA 210 3.4353434E-8 22.62183 4 TACATGG 6745 0.0 22.53798 2 GTACTTT 6765 0.0 22.502838 14-15 GATATGA 240 5.9062586E-9 21.77351 2 GTTCGAC 55 0.0028781316 21.590086 16-17 GGTATCA 8920 0.0 21.469757 1 GTAAGGT 200 5.371512E-7 21.377628 3 CTTACAC 290 1.05501385E-10 21.295721 3 >>END_MODULE