FastQCFastQC Report
Thu 26 May 2016
SRR937269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638612
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80421.2592935929797748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56680.8875498737887794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.6997989389488454No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26870.42075626515004416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19350.3030008831653649No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15840.24803793226560103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.21906885558054032No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12930.202470357588019No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10260.16066093339930976No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10000.15658960370303096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.14844694431047334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9410.1473508170845521No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8930.13983451610680664No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8880.1390515680882915No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.12057399485133384No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7350.11509335872172774No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7130.11164838744026105No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7130.11164838744026105No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6520.10209642161437618No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6460.101156883992158No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.10037393597364284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG902.237357E-1047.5271575
GTACGTG856.373867E-427.9637371
GTACATG73200.027.0163331
TACATGG74150.026.0911542
GTATCAA110500.025.2964291
AGTACTT54150.024.8290112-13
ATGGGAT20700.024.796785
GAGTACT53050.024.26919612-13
ACATGGG76000.024.2676753
ATGGGAG16500.023.9076025
TACCTGG5350.023.1009372
GTAAGGT1652.413386E-623.045284
ACGTGGG1050.002185879522.6355323
TAAGGTG1703.1297677E-622.3657235
GTACTTT57550.022.28983914-15
CATGGGA58700.022.1864814
CATGGGC7100.022.0918224
TGGGATG10250.021.7843916
TACTTTT61450.021.76431314-15
TGGGATA4600.021.688676
Produced by FastQC (version 0.11.5)