##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937269_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 638612 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74830726638397 33.0 31.0 34.0 30.0 34.0 2 31.778042066231137 33.0 31.0 34.0 30.0 34.0 3 31.806016798932685 33.0 31.0 34.0 30.0 34.0 4 35.472742447683416 37.0 35.0 37.0 33.0 37.0 5 35.47669946696899 37.0 35.0 37.0 33.0 37.0 6 35.59724840748373 37.0 35.0 37.0 33.0 37.0 7 35.548862219939494 37.0 35.0 37.0 33.0 37.0 8 35.54407057806618 37.0 35.0 37.0 33.0 37.0 9 37.13375727358709 39.0 37.0 39.0 34.0 39.0 10-11 37.039838744026106 39.0 37.0 39.0 33.0 39.0 12-13 36.93372501612873 39.0 37.0 39.0 33.0 39.0 14-15 37.96993479608902 40.0 38.0 41.0 33.0 41.0 16-17 37.96621501005305 40.0 38.0 41.0 33.0 41.0 18-19 38.03306076302982 40.0 38.0 41.0 33.0 41.0 20-21 38.026967078601714 40.0 38.0 41.0 33.0 41.0 22-23 37.897469825183364 40.0 38.0 41.0 33.0 41.0 24-25 37.73795982537128 40.0 37.0 41.0 33.0 41.0 26-27 37.56405141149868 40.0 37.0 41.0 32.5 41.0 28-29 37.38462947767972 39.5 37.0 41.0 32.0 41.0 30-31 37.254804169041606 39.0 36.5 41.0 32.0 41.0 32-33 36.997269077311415 39.0 35.5 41.0 32.0 41.0 34-35 36.75236058827582 39.0 35.0 41.0 31.0 41.0 36-37 36.68086882175719 39.0 35.0 41.0 31.0 41.0 38-39 36.589254508214694 39.0 35.0 41.0 31.0 41.0 40-41 36.37422723030573 39.0 35.0 41.0 30.5 41.0 42-43 36.02775942199646 39.0 35.0 41.0 30.0 41.0 44-45 35.623836539244486 39.0 35.0 41.0 28.0 41.0 46-47 35.064733359222814 39.0 35.0 40.5 25.0 41.0 48-49 34.5832038859276 38.5 35.0 40.0 23.0 41.0 50-51 33.584844944974414 37.5 33.5 39.5 21.0 40.5 52-53 33.68263828427903 38.0 33.5 39.5 20.0 40.5 54-55 34.02041145484269 38.0 34.0 40.0 20.0 41.0 56-57 33.807408097561584 38.0 34.0 40.0 18.5 41.0 58-59 33.57789314951802 38.0 34.0 40.0 16.0 41.0 60-61 33.28332696535611 37.5 33.0 40.0 13.0 41.0 62-63 32.97949224255103 37.0 33.0 40.0 9.0 41.0 64-65 32.62624018966133 36.5 33.0 39.5 7.5 41.0 66-67 32.224076591106964 36.0 32.0 39.0 4.0 41.0 68-69 31.742651249898216 35.0 31.5 39.0 2.0 40.5 70-71 31.321000858111027 35.0 31.0 38.5 2.0 40.0 72-73 30.918837259556664 35.0 31.0 37.5 2.0 40.0 74-75 30.47861612371831 35.0 30.5 37.0 2.0 39.0 76-77 30.052676742685698 35.0 30.0 36.5 2.0 39.0 78-79 29.59497081169787 34.5 29.5 36.0 2.0 38.5 80-81 29.152523754642882 34.0 29.0 35.5 2.0 37.0 82-83 28.70874412006038 34.0 29.0 35.0 2.0 37.0 84-85 28.24646577264442 34.0 28.5 35.0 2.0 36.5 86-87 27.828380143185534 34.0 27.0 35.0 2.0 36.0 88-89 27.429692050885357 34.0 26.5 35.0 2.0 36.0 90-91 26.980937564593212 33.5 25.5 35.0 2.0 35.0 92-93 26.557978083719064 33.0 25.0 35.0 2.0 35.0 94-95 26.03877628356498 33.0 24.0 35.0 2.0 35.0 96-97 25.435947022605276 32.5 20.5 35.0 2.0 35.0 98-99 24.777711662167327 32.0 10.0 35.0 2.0 35.0 100-101 23.08223224743663 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22130796703718403 1101 2 0.3328779862569782 1101 3 0.34973889164659155 1101 4 0.2918697872427103 1101 5 0.45264381448744473 1101 6 0.14084307401387974 1101 7 0.21329788182157472 1101 8 0.231565204119498 1101 9 0.24439256942437027 1101 10-11 0.17444269844017413 1101 12-13 0.2642305995499399 1101 14-15 0.4962060310320453 1101 16-17 0.39631249750905795 1101 18-19 0.447980392503041 1101 20-21 0.34277858580363585 1101 22-23 0.3698847699793575 1101 24-25 0.406730575279326 1101 26-27 0.512099269454275 1101 28-29 0.5695449111112367 1101 30-31 0.5266321214244627 1101 32-33 0.48473775719936896 1101 34-35 0.2855400154994854 1101 36-37 0.43420400086277056 1101 38-39 0.6758899805512542 1101 40-41 0.404109728235845 1101 42-43 0.6471047615278707 1101 44-45 0.4222465201861496 1101 46-47 0.5698977207436258 1101 48-49 0.49344992965727386 1101 50-51 0.4110534788539084 1101 52-53 0.5951016015414368 1101 54-55 0.6320448629528812 1101 56-57 0.4893511503361694 1101 58-59 0.6419793024643283 1101 60-61 0.5755614314411943 1101 62-63 0.3146522137899126 1101 64-65 0.2575940873033744 1101 66-67 0.5106762965572429 1101 68-69 0.5282983967964547 1101 70-71 0.5492199841256671 1101 72-73 0.7631540794679346 1101 74-75 0.711582989343718 1101 76-77 0.5860685296697952 1101 78-79 0.7007855269069836 1101 80-81 0.6493008340248032 1101 82-83 0.8037126860632284 1101 84-85 0.6480620851339332 1101 86-87 0.7169043407111566 1101 88-89 0.7257674141055155 1101 90-91 0.9139956340089803 1101 92-93 0.8039784609160527 1101 94-95 0.772045059703192 1101 96-97 0.9937501639521926 1101 98-99 1.0933679323753935 1101 100-101 1.6434619280806402 1102 1 0.1714405653225448 1102 2 0.06597437583240406 1102 3 0.16971149354071713 1102 4 0.17136797930010772 1102 5 0.18468606550062816 1102 6 0.29218555745986663 1102 7 0.11135271127192681 1102 8 0.2856301665954888 1102 9 0.24505021893279633 1102 10-11 0.18031406029697905 1102 12-13 0.32875385114005695 1102 14-15 0.6310989671165856 1102 16-17 0.40997475056404653 1102 18-19 0.3286941677673454 1102 20-21 0.4136136107444557 1102 22-23 0.5321318894982525 1102 24-25 0.4723768168242586 1102 26-27 0.6708361550446327 1102 28-29 0.4393018182117103 1102 30-31 0.5366238829992156 1102 32-33 0.3444672899358352 1102 34-35 0.5530704110151206 1102 36-37 0.6440547357920323 1102 38-39 0.7776547129686193 1102 40-41 0.9657792083128669 1102 42-43 0.7081237146543202 1102 44-45 0.6147443299996098 1102 46-47 0.9392533904183793 1102 48-49 0.6250724245943218 1102 50-51 0.5020773940289942 1102 52-53 0.43125190650561507 1102 54-55 0.46500307490595816 1102 56-57 0.4161181975848436 1102 58-59 0.6686639654605031 1102 60-61 0.6981121618595054 1102 62-63 0.8594103673380147 1102 64-65 0.8648218215939778 1102 66-67 0.8496281409435937 1102 68-69 0.9013198095795509 1102 70-71 0.6013749140040865 1102 72-73 0.5157555937237355 1102 74-75 0.5739549910436352 1102 76-77 0.8083410054946505 1102 78-79 0.5067587413410877 1102 80-81 0.36203145651261437 1102 82-83 0.5337736789003102 1102 84-85 0.4144243811616839 1102 86-87 0.27314287042528207 1102 88-89 0.41868782901824986 1102 90-91 0.4113104296984069 1102 92-93 0.040665806742349986 1102 94-95 0.13903604017076177 1102 96-97 0.5452889925763316 1102 98-99 0.44372299188436415 1102 100-101 0.27565897601783007 1103 1 0.32286315295961643 1103 2 0.2752470316036941 1103 3 0.43366757669189937 1103 4 0.23126207402953725 1103 5 0.13504639949548647 1103 6 0.37273483816370856 1103 7 0.3020416442626086 1103 8 0.33566994966344765 1103 9 0.3244082939810369 1103 10-11 0.33051914887145983 1103 12-13 0.43262424064830185 1103 14-15 0.43984758385473555 1103 16-17 0.5732773853920605 1103 18-19 0.4459296103654111 1103 20-21 0.2986748400869885 1103 22-23 0.4074782765555014 1103 24-25 0.29584745509099264 1103 26-27 0.5338932323152363 1103 28-29 -0.04188925026089407 1103 30-31 0.19152977546603722 1103 32-33 0.5825095057155352 1103 34-35 0.4518476532711375 1103 36-37 0.5574156733920574 1103 38-39 0.48837350612410546 1103 40-41 0.4209352311035346 1103 42-43 0.4718157205440434 1103 44-45 0.49504530948618 1103 46-47 0.7023577958215554 1103 48-49 0.6916770540630566 1103 50-51 0.6975505838994067 1103 52-53 0.5504524954779342 1103 54-55 0.8233913576492569 1103 56-57 0.9509055182180788 1103 58-59 0.8789895007028221 1103 60-61 1.0000222063982491 1103 62-63 1.1090445460234335 1103 64-65 0.8809636870997721 1103 66-67 0.8538545203185919 1103 68-69 0.9028836956993693 1103 70-71 1.1065032715578447 1103 72-73 1.1555987935627776 1103 74-75 1.0757223652696943 1103 76-77 1.1549452691420719 1103 78-79 1.0513340685225216 1103 80-81 1.0389392146681438 1103 82-83 0.9870571538369788 1103 84-85 1.0025701701010767 1103 86-87 0.979049970102448 1103 88-89 1.0933755090709276 1103 90-91 1.1264339401197425 1103 92-93 1.0057470190741888 1103 94-95 1.1057805962923233 1103 96-97 1.2335280659509422 1103 98-99 1.2192950449650368 1103 100-101 1.3345539212818132 1104 1 0.11514601203530361 1104 2 0.02009413596320897 1104 3 0.28863507941071376 1104 4 0.006264428904984243 1104 5 0.10631477822796143 1104 6 0.08847568197980848 1104 7 0.08245164948615269 1104 8 0.08424203882331227 1104 9 0.13548519993159402 1104 10-11 0.17301330449876673 1104 12-13 -0.005618080316942553 1104 14-15 0.045761318344673896 1104 16-17 -0.04637169535019581 1104 18-19 -0.03641631655637667 1104 20-21 -0.025242559445466384 1104 22-23 -0.07329737660132452 1104 24-25 -0.13050702645106327 1104 26-27 -0.006030469720229803 1104 28-29 0.006842756349882961 1104 30-31 -0.4265468907153789 1104 32-33 -0.311861485644215 1104 34-35 -0.5182539917163993 1104 36-37 -0.4966704636702559 1104 38-39 -0.4151633027199537 1104 40-41 -0.42729625743464794 1104 42-43 -0.38081510349110204 1104 44-45 -0.2571468654767841 1104 46-47 -0.23289704451788396 1104 48-49 0.022975917728608408 1104 50-51 -0.21401849101989256 1104 52-53 -0.07912773726939548 1104 54-55 0.10058374669180381 1104 56-57 0.0028348034491543217 1104 58-59 -0.20990169548827708 1104 60-61 -0.3989729040239993 1104 62-63 -0.2838792673334183 1104 64-65 -0.5105150890500454 1104 66-67 -0.2606962550776686 1104 68-69 0.01607185452929727 1104 70-71 -0.0700888383249314 1104 72-73 -0.2235778991645141 1104 74-75 -0.00266715681903662 1104 76-77 -0.09353048420399546 1104 78-79 -0.10839927394486892 1104 80-81 -0.18118704947181286 1104 82-83 -0.2244995044663156 1104 84-85 -0.023955361030377986 1104 86-87 -0.11574875227058357 1104 88-89 0.012749925704053311 1104 90-91 0.03682236612099743 1104 92-93 0.3063415180851372 1104 94-95 0.06953906313995972 1104 96-97 0.4069828394975552 1104 98-99 0.811461658825408 1104 100-101 0.9367833460927812 1105 1 0.19807448009401796 1105 2 0.23370363909876346 1105 3 0.32780148765273154 1105 4 0.18035865052996058 1105 5 0.30820257024780773 1105 6 0.28768447186334356 1105 7 0.061560170608053966 1105 8 -0.017906762086340677 1105 9 0.10974395191284714 1105 10-11 0.22400587048318243 1105 12-13 0.23905298818281295 1105 14-15 0.3245950479291295 1105 16-17 0.2794490182463818 1105 18-19 0.3080034339776958 1105 20-21 0.484915682577828 1105 22-23 0.5505203306552389 1105 24-25 0.49604029110113856 1105 26-27 0.3743544537860757 1105 28-29 0.09172233046606948 1105 30-31 0.2140361152800736 1105 32-33 0.19878345612410442 1105 34-35 0.43362949322605715 1105 36-37 0.42834762605478005 1105 38-39 0.3545814168031711 1105 40-41 0.6455891829209506 1105 42-43 0.356902188563204 1105 44-45 0.1637019733351508 1105 46-47 0.29983243656543124 1105 48-49 0.5509816116849251 1105 50-51 0.2757328183316403 1105 52-53 0.39243263072729917 1105 54-55 0.28051836525703777 1105 56-57 0.22168601976780877 1105 58-59 0.31011006283299736 1105 60-61 0.5014926910418112 1105 62-63 0.44778873452724355 1105 64-65 0.33761520865514427 1105 66-67 0.3942928480165335 1105 68-69 0.6117277362741866 1105 70-71 0.3229815048629945 1105 72-73 0.2091746247828361 1105 74-75 0.36279266676307387 1105 76-77 0.20269199357148437 1105 78-79 0.238635949334018 1105 80-81 0.30262264662080796 1105 82-83 0.20593042799871597 1105 84-85 0.20681784550260218 1105 86-87 0.021363826118225404 1105 88-89 0.14217006387509912 1105 90-91 0.4934167169582899 1105 92-93 0.04063360684539319 1105 94-95 0.25946825478767366 1105 96-97 0.12088630066494588 1105 98-99 0.34084777108507325 1105 100-101 0.7524049004308679 1106 1 0.2111549066658931 1106 2 0.31052453075247044 1106 3 0.02632450105716444 1106 4 0.23754733082322588 1106 5 0.24931982196964952 1106 6 0.0581637172162317 1106 7 0.09523092895805263 1106 8 0.16773948319248433 1106 9 0.24882647061239282 1106 10-11 0.16215271748015425 1106 12-13 -0.030492436907898934 1106 14-15 -0.13979480988025017 1106 16-17 -0.11662847379241725 1106 18-19 0.1737092030660463 1106 20-21 0.0988602689660425 1106 22-23 0.22827943974211706 1106 24-25 0.13215240507580717 1106 26-27 0.5111747437216465 1106 28-29 0.5764896044051966 1106 30-31 0.476755510969916 1106 32-33 0.681456288087368 1106 34-35 0.5082217134734108 1106 36-37 0.36507002107810393 1106 38-39 0.42423144827765924 1106 40-41 0.5453232697905364 1106 42-43 0.6291553452691261 1106 44-45 0.35862624942194543 1106 46-47 0.26654816640324697 1106 48-49 0.35320033159901953 1106 50-51 0.26997252432290253 1106 52-53 0.28558137545069684 1106 54-55 0.3821971588584532 1106 56-57 0.3559709934773636 1106 58-59 0.3620880954924033 1106 60-61 0.3641782979439796 1106 62-63 0.5905729769178194 1106 64-65 0.6001332601992431 1106 66-67 0.781444082927294 1106 68-69 0.7152186271873084 1106 70-71 0.525873917660153 1106 72-73 0.6152012947821 1106 74-75 0.7215509056729452 1106 76-77 0.5497086287636641 1106 78-79 0.6804579055659694 1106 80-81 0.7570355548634211 1106 82-83 0.8677856638722226 1106 84-85 0.8349560370155977 1106 86-87 0.5929115840238772 1106 88-89 0.33951394116162703 1106 90-91 0.8564922834990583 1106 92-93 1.0820086716458057 1106 94-95 0.9126147777825651 1106 96-97 1.0655872467510008 1106 98-99 1.3552022200964018 1106 100-101 1.092237946790938 1107 1 0.08204261683172831 1107 2 0.03297124126518369 1107 3 0.06555162832939132 1107 4 -0.10064076917883824 1107 5 0.2405261089690569 1107 6 0.25905457455730385 1107 7 0.1708021872079044 1107 8 0.25760626808880005 1107 9 0.06822455611907685 1107 10-11 0.11655198604906047 1107 12-13 0.1795148911862654 1107 14-15 0.26054540665459314 1107 16-17 0.19290539969113496 1107 18-19 0.2938566378885028 1107 20-21 0.5220301406210197 1107 22-23 0.34207049946722634 1107 24-25 0.332764151620232 1107 26-27 0.5094139336341428 1107 28-29 0.2899840945513219 1107 30-31 0.39550899985487575 1107 32-33 0.3269201059095721 1107 34-35 0.3348766796929681 1107 36-37 0.3267043468819608 1107 38-39 0.5156660777724298 1107 40-41 0.6436208087913826 1107 42-43 0.6100069662940939 1107 44-45 0.5466194313080024 1107 46-47 0.37185448763105455 1107 48-49 0.020719645376978235 1107 50-51 -0.17358621873665925 1107 52-53 -0.074662220507129 1107 54-55 0.1117261622606236 1107 56-57 0.017019071747832015 1107 58-59 0.1486671160087738 1107 60-61 0.09936187933751484 1107 62-63 0.22199281239176827 1107 64-65 0.11217867141099447 1107 66-67 0.2444843001512993 1107 68-69 0.11472151133220976 1107 70-71 0.3326947882561164 1107 72-73 0.3764248797592735 1107 74-75 0.2685173273806427 1107 76-77 0.30834785451261126 1107 78-79 0.2786727450715176 1107 80-81 0.5084018194420779 1107 82-83 0.5156454698500212 1107 84-85 0.5798395800457534 1107 86-87 0.6079514061280697 1107 88-89 0.5144279681446342 1107 90-91 0.3915327488215503 1107 92-93 0.7354575851811838 1107 94-95 0.6795331418946589 1107 96-97 0.7009192277944365 1107 98-99 0.9646113551745401 1107 100-101 1.4337799707691943 1108 1 0.10137636688647333 1108 2 -0.04600211112585484 1108 3 -0.02611248359614038 1108 4 -0.14351264327058288 1108 5 -0.05896387180070661 1108 6 0.07292144401069578 1108 7 0.052659676448129744 1108 8 -0.030962692005481074 1108 9 0.17238433825606592 1108 10-11 0.21194668360088542 1108 12-13 0.22988575997077731 1108 14-15 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-1.4333087724575577 2316 54-55 -1.2897779075331854 2316 56-57 -1.2934497599075527 2316 58-59 -1.4482956318358262 2316 60-61 -1.5943536240593517 2316 62-63 -1.576836957833713 2316 64-65 -1.7238101894524043 2316 66-67 -1.6275874408518405 2316 68-69 -1.681299611983377 2316 70-71 -1.7618399237084326 2316 72-73 -2.0769663764480235 2316 74-75 -1.9796327121122275 2316 76-77 -1.8299535464700547 2316 78-79 -1.7592833617668333 2316 80-81 -1.7600792919898538 2316 82-83 -1.743467121812813 2316 84-85 -2.0705304120556747 2316 86-87 -2.0236600699507044 2316 88-89 -2.123899681874576 2316 90-91 -2.0179892084975144 2316 92-93 -2.02947274748956 2316 94-95 -2.1220659016859003 2316 96-97 -2.2408783062278914 2316 98-99 -2.442273767110173 2316 100-101 -2.390056779313724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2200.0 3 374.0 4 306.0 5 313.0 6 406.0 7 504.0 8 682.0 9 901.0 10 1160.0 11 1675.0 12 2583.0 13 3559.0 14 4496.0 15 5910.0 16 8285.0 17 11482.0 18 9026.0 19 4788.0 20 4453.0 21 4467.0 22 4865.0 23 5280.0 24 5897.0 25 6656.0 26 7464.0 27 8956.0 28 10674.0 29 12804.0 30 15268.0 31 19161.0 32 24362.0 33 31519.0 34 42777.0 35 62076.0 36 85449.0 37 115305.0 38 98563.0 39 13964.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.281552604955415 19.43159753423108 15.167400825693262 19.11944903512024 2 14.869512505378744 22.07498515938351 40.804314300700725 22.25118803453702 3 19.381591924166568 29.20501158985119 29.972799969847543 21.440596516134704 4 11.555905234824285 17.224949511129537 38.436870852139066 32.78227440190712 5 11.915446868513033 40.30594139316076 32.45940199794615 15.319209740380055 6 28.141663188222036 37.69132753139231 18.25744047152017 15.909568808865481 7 26.836311135174157 36.191238436006685 20.19930478723822 16.773145641580943 8 27.48148624748717 32.81175213306363 19.504134306505197 20.202627312944013 9 28.850288275338254 16.759986064861167 20.507879316676032 33.88184634312454 10-11 24.69732744191959 28.477782041232047 25.7015670940845 21.123323422763864 12-13 27.41201793187113 26.51832775022187 26.814315050703126 19.25533926720387 14-15 23.792779073608134 26.2723001158794 28.29881664928868 21.636104161223788 16-17 20.06821910778446 30.50305903142049 28.031195293870248 21.397526566924796 18-19 20.068036817709658 27.72335564614578 32.95423519775554 19.25437233838902 20-21 22.239369940355232 25.79867654835526 32.606433977275046 19.355519534014466 22-23 21.139950195436622 26.629279246058708 32.47990321532863 19.750867343176036 24-25 20.707509720178116 26.764302547800682 33.010164215309395 19.518023516711803 26-27 20.423391098916532 26.634204747045747 33.401620532922614 19.540783621115104 28-29 19.872076026259567 27.269132299783365 33.24626698794122 19.61252468601585 30-31 20.064735076603174 27.038703729132745 33.13062753790925 19.765933656354825 32-33 19.758724538878873 27.43790381938396 32.772439292714814 20.03093234902236 34-35 20.332952279925966 27.05013663785359 32.912814614248155 19.704096467972292 36-37 20.47627304417279 26.786200446741354 32.8146195990312 19.92290691005466 38-39 20.35683774261648 27.162768877623467 32.78700742215601 19.693385957604043 40-41 20.02232661181506 27.5706983224446 32.57049435480328 19.83648071093706 42-43 20.007389979908982 27.72019119807892 31.675870146599316 20.596548675412784 44-45 20.222033737390063 27.648370996735167 31.477272103137643 20.652323162737122 46-47 20.71214498666248 28.15691197238349 30.733328102934255 20.39761493801977 48-49 20.66354912868475 28.385497171461978 30.137464594236217 20.813489105617062 50-51 20.55256942428261 28.744203199912118 30.080350907478753 20.62287646832652 52-53 20.76526660498579 29.237048389666175 29.219784022770128 20.777900982577897 54-55 20.134013839255587 28.919382961927138 29.535856039097702 21.410747159719573 56-57 20.928940958671465 29.08765007432626 28.85106115933275 21.13234780766953 58-59 21.189199194943768 28.431646302341594 29.279998619545044 21.099155883169587 60-61 20.935758112011094 28.833153702666536 29.920978194876525 20.310109990445845 62-63 20.971170651045018 28.879866207723175 29.757093594977142 20.39186954625466 64-65 20.59519327309237 29.272480547188756 29.675341992971887 20.456984186746986 66-67 20.748734000949256 29.16422488693285 29.126803877036288 20.960237235081607 68-69 20.707905041605482 29.045631110609083 29.413836489827677 20.832627357957755 70-71 20.896969544831613 29.23611443417932 29.129898278811915 20.737017742177148 72-73 20.77128636122557 29.009895086350557 29.20658856463189 21.01222998779198 74-75 20.285972490033245 30.049861226385342 28.900997695212666 20.763168588368746 76-77 20.239104902215384 29.481785080186757 29.16325052742575 21.115859490172113 78-79 20.303575687583947 29.402137755294177 29.212667737845678 21.0816188192762 80-81 20.34977898580567 29.95273786971341 28.795305948752947 20.902177195727976 82-83 20.167842416102328 29.63877086066114 28.912295786758385 21.28109093647815 84-85 20.19972013140019 29.34698372557817 28.762616834159026 21.69067930886261 86-87 20.39077287777679 29.401627069823117 28.725367839994604 21.482232212405485 88-89 20.371089505671623 29.00981345107278 28.590335292257897 22.028761750997703 90-91 20.19456837153914 28.789639128309847 29.03526188909085 21.98053061106016 92-93 20.372702090153613 29.14761302935378 29.011501610978662 21.468183269513947 94-95 20.08129009785878 28.784241551305644 29.154055796920673 21.9804125539149 96-97 19.901079418871003 28.87696507577897 29.21169475038439 22.010260754965643 98-99 20.085655154450727 29.079760184396296 28.616572758240327 22.21801190291265 100-101 20.019107276060137 28.63824577008362 29.46583554722031 21.876811406635934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1779.0 1 1105.0 2 622.0 3 817.0 4 725.5 5 757.5 6 1236.0 7 2453.0 8 3766.5 9 4049.0 10 3314.0 11 2640.5 12 2523.5 13 2544.5 14 2551.5 15 2465.0 16 2405.0 17 2426.0 18 2397.0 19 2383.5 20 2502.0 21 2671.0 22 2951.5 23 3325.5 24 3668.0 25 4198.5 26 4819.0 27 5629.5 28 6408.5 29 7363.5 30 8659.5 31 9506.0 32 10836.5 33 12519.5 34 14039.0 35 15295.0 36 16971.0 37 18752.0 38 19204.0 39 18951.0 40 19023.5 41 19543.5 42 19694.5 43 20234.5 44 21096.0 45 21621.5 46 21447.0 47 23080.0 48 29170.5 49 28574.5 50 22406.5 51 18830.0 52 16211.5 53 15406.5 54 14784.5 55 13661.5 56 12318.0 57 11122.0 58 10343.0 59 9685.0 60 8855.5 61 8066.0 62 6835.0 63 4743.0 64 3020.5 65 2268.5 66 1667.0 67 1234.5 68 894.0 69 562.0 70 414.5 71 310.5 72 262.5 73 256.5 74 199.0 75 142.5 76 101.5 77 64.0 78 41.0 79 26.5 80 18.0 81 10.5 82 6.0 83 4.0 84 1.5 85 2.0 86 2.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3219482252134316 2 0.2890644084357951 3 0.2893775876432012 4 0.28781169160617087 5 0.2752845233099284 6 0.2120223234139039 7 0.21875567637313423 8 0.21656342192129177 9 0.21515411548796454 10-11 0.22133940483423423 12-13 0.22274871126756152 14-15 0.20536726525652507 16-17 0.21930373998609481 18-19 0.20513238085097052 20-21 0.20967347935835845 22-23 0.2068548664917039 24-25 0.20012151353247357 26-27 0.21178743900834937 28-29 0.21272697663056753 30-31 0.21914715038239183 32-33 0.22157428923978878 34-35 0.21037813257502208 36-37 0.2083424677268827 38-39 0.1978509642787796 40-41 0.19667654225100686 42-43 0.19769437467507658 44-45 0.21397969346019177 46-47 0.2054455600583766 48-49 0.21249209222501297 50-51 0.22032157241016456 52-53 0.22885570581197973 54-55 0.22533243972866152 56-57 0.19096102171584625 58-59 0.17866873782515832 60-61 0.18681139721771592 62-63 0.1888470620658553 64-65 0.18352301553995226 66-67 0.19879050190099778 68-69 0.18728116602882502 70-71 0.19299668656398564 72-73 0.20763781451021904 74-75 0.19464087740286745 76-77 0.20646339248244627 78-79 0.20481920164356449 80-81 0.18939512567881595 82-83 0.20168740956950387 84-85 0.1832098363325462 86-87 0.1892385360751129 88-89 0.19926027071210686 90-91 0.19612847863804628 92-93 0.1983207330898887 94-95 0.19808584868433415 96-97 0.19291839176213413 98-99 0.2018439991732069 100-101 0.18140905588996137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 638612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.49155787244769 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33709382898559 41.90373188112548 2 12.179594182351023 13.030109341363138 3 3.9206412686966736 6.291636279647846 4 1.720431813342059 3.6811431163594746 5 0.9765670275663779 2.611904583569556 6 0.5850291976126899 1.8776473908702505 7 0.3971654476903368 1.4871498971044972 8 0.2749424478299249 1.1765679887749485 9 0.21975858326655426 1.0579706077292999 >10 1.2337928728834766 12.216459863527831 >50 0.0969744592646362 3.4659410401495783 >100 0.05009856998646941 4.747723543080743 >500 0.005273533682784534 2.047813287256121 >1k 0.002050818654416208 2.255619242632383 >5k 5.859481869760594E-4 2.1485819368088634 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8042 1.2592935929797748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5668 0.8875498737887794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4469 0.6997989389488454 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2687 0.42075626515004416 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1935 0.3030008831653649 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1584 0.24803793226560103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1399 0.21906885558054032 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1293 0.202470357588019 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1026 0.16066093339930976 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1000 0.15658960370303096 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 948 0.14844694431047334 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 941 0.1473508170845521 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 893 0.13983451610680664 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 888 0.1390515680882915 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 770 0.12057399485133384 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 735 0.11509335872172774 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 713 0.11164838744026105 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 713 0.11164838744026105 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 652 0.10209642161437618 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 646 0.101156883992158 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.10037393597364284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 7.829480185151548E-5 0.0 0.0 0.0 0.0 18-19 1.5658960370303095E-4 0.0 0.0 0.0 0.0 20-21 1.5658960370303095E-4 0.0 0.0 0.0 0.0 22-23 3.131792074060619E-4 0.0 0.0 1.5658960370303095E-4 0.0 24-25 6.263584148121238E-4 0.0 0.0 4.6976881110909283E-4 0.0 26-27 6.263584148121238E-4 0.0 0.0 5.480636129606084E-4 0.0 28-29 7.829480185151548E-4 0.0 0.0 8.612428203666702E-4 0.0 30-31 7.829480185151548E-4 0.0 0.0 0.0019573700462878867 0.0 32-33 7.829480185151548E-4 0.0 0.0 0.004462803705536382 0.0 34-35 8.612428203666702E-4 0.0 0.0 0.005558930931457599 0.0 36-37 9.395376222181857E-4 0.0 0.0 0.00743800617589397 0.0 38-39 9.395376222181857E-4 0.0 0.0 0.010256619042548527 0.0 40-41 9.395376222181857E-4 0.0 0.0 0.01605043437956067 0.0 42-43 9.395376222181857E-4 0.0 0.0 0.022392313329533425 0.0 44-45 9.395376222181857E-4 0.0 0.0 0.03421482840911226 0.0 46-47 9.395376222181857E-4 0.0 0.0 0.06146141945343965 0.0 48-49 0.0010961272259212165 0.0 0.0 0.08628087164037004 0.0 50-51 0.0010961272259212165 0.0 0.0 0.12026081564392777 0.0 52-53 0.0010961272259212165 0.0 0.0 0.16402760987892492 0.0 54-55 0.0010961272259212165 0.0 0.0 0.20301842120097963 0.0 56-57 0.0010961272259212165 0.0 0.0 0.2517960827544738 0.0 58-59 0.0010961272259212165 0.0 0.0 0.33056065341709834 0.0 60-61 0.0010961272259212165 0.0 0.0 0.4368066995296048 0.0 62-63 0.0010961272259212165 0.0 0.0 0.5672458394142297 0.0 64-65 0.0010961272259212165 0.0 0.0 0.675449255573024 0.0 66-67 0.0012527168296242476 0.0 0.0 0.8359535993686307 0.0 68-69 0.0012527168296242476 0.0 0.0 1.0318471936011224 0.0 70-71 0.0012527168296242476 0.0 0.0 1.1892197453226685 0.0 72-73 0.0013310116314757632 0.0 0.0 1.4320902206660695 0.0 74-75 0.0014093064333272785 0.0 0.0 1.6423900584392401 0.0 76-77 0.0014093064333272785 0.0 0.0 1.8383619474735835 0.0 78-79 0.0014093064333272785 0.0 0.0 2.0822502552410542 0.0 80-81 0.0015658960370303096 0.0 0.0 2.332245557552943 0.0 82-83 0.0015658960370303096 0.0 0.0 2.6606139565181985 0.0 84-85 0.0015658960370303096 0.0 0.0 3.0524794397850337 0.0 86-87 0.0015658960370303096 0.0 0.0 3.5429963733847782 0.0 88-89 0.0015658960370303096 0.0 0.0 4.106014293499026 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 90 2.237357E-10 47.527157 5 GTACGTG 85 6.373867E-4 27.963737 1 GTACATG 7320 0.0 27.016333 1 TACATGG 7415 0.0 26.091154 2 GTATCAA 11050 0.0 25.296429 1 AGTACTT 5415 0.0 24.82901 12-13 ATGGGAT 2070 0.0 24.79678 5 GAGTACT 5305 0.0 24.269196 12-13 ACATGGG 7600 0.0 24.267675 3 ATGGGAG 1650 0.0 23.907602 5 TACCTGG 535 0.0 23.100937 2 GTAAGGT 165 2.413386E-6 23.04528 4 ACGTGGG 105 0.0021858795 22.635532 3 TAAGGTG 170 3.1297677E-6 22.365723 5 GTACTTT 5755 0.0 22.289839 14-15 CATGGGA 5870 0.0 22.186481 4 CATGGGC 710 0.0 22.091822 4 TGGGATG 1025 0.0 21.784391 6 TACTTTT 6145 0.0 21.764313 14-15 TGGGATA 460 0.0 21.68867 6 >>END_MODULE