FastQCFastQC Report
Thu 26 May 2016
SRR937269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638612
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99941.5649564994080913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75051.1752049757912473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62490.9785284335402403No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30100.47133470714612313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17530.2745015752914132No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.268081401539589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.2544581060174253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.22157428923978878No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14090.2206347516175706No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12180.1907261373102917No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10590.16582839032150978No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.1578423205326552No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9540.14938648193269152No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9470.1482903547067703No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8860.13873838888088544No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.1340407007697945No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8540.13372752156238843No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.13247480473276418No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8090.12668098939575204No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7290.11415382109950956No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6910.10820341615879439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT1054.6209607E-836.2179681
TACCTGG5100.028.8741192
GTATAGA1658.3364284E-825.9287741
GTATCAA117000.025.0681741
CATATAG1751.483204E-724.42993
AGTACTT68350.024.01003312-13
GTATTAG2052.6431735E-823.1883341
GAGTACT66650.023.09022112-13
GTACATG69250.022.9957941
TACATGG71000.022.279382
GGTATCA85900.022.0802061
GTATAGG2606.184564E-1021.939731
ATGGGAG16600.021.748175
ACATGGG70500.021.6962153
TGTATCG1100.002876379521.5903939
GTACTTT72050.021.29373214-15
TACTTTT76050.021.2042914-15
CATGGGG14700.021.0045434
TATCAAC140050.020.8936772
ATCAACG140450.020.7665273
Produced by FastQC (version 0.11.5)