##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 638612 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12922557045593 33.0 31.0 34.0 31.0 34.0 2 32.30821844876075 33.0 31.0 34.0 31.0 34.0 3 32.365738507888985 34.0 31.0 34.0 30.0 34.0 4 36.00965218317226 37.0 35.0 37.0 35.0 37.0 5 35.93122271426155 37.0 35.0 37.0 35.0 37.0 6 36.04107501894734 37.0 35.0 37.0 35.0 37.0 7 35.993202445303254 37.0 35.0 37.0 35.0 37.0 8 35.988083531158196 37.0 35.0 37.0 35.0 37.0 9 37.64366626370942 39.0 37.0 39.0 35.0 39.0 10-11 37.58113298841863 39.0 37.0 39.0 35.0 39.0 12-13 37.494419929472045 39.0 37.0 39.0 35.0 39.0 14-15 38.57492499357983 40.0 38.0 41.0 34.0 41.0 16-17 38.59774792831954 40.0 38.0 41.0 34.5 41.0 18-19 38.72143022680439 40.0 38.0 41.0 35.0 41.0 20-21 38.68544280408135 40.0 38.0 41.0 34.0 41.0 22-23 38.55362645863216 40.0 38.0 41.0 34.0 41.0 24-25 38.400021452775704 40.0 38.0 41.0 34.0 41.0 26-27 38.28081526811272 40.0 38.0 41.0 34.0 41.0 28-29 38.16474479026388 40.0 37.5 41.0 34.0 41.0 30-31 38.06127899256513 40.0 37.0 41.0 34.0 41.0 32-33 37.8329838775344 40.0 36.0 41.0 33.0 41.0 34-35 37.63862016373009 40.0 36.0 41.0 33.0 41.0 36-37 37.41538993943114 40.0 35.0 41.0 33.0 41.0 38-39 37.22986962349596 40.0 35.0 41.0 33.0 41.0 40-41 36.98996495524669 40.0 35.0 41.0 32.5 41.0 42-43 36.66091382560929 39.0 35.0 41.0 31.5 41.0 44-45 36.1539620301529 39.0 35.0 41.0 30.5 41.0 46-47 35.499450370491004 39.0 35.0 41.0 27.5 41.0 48-49 34.952699761357444 39.0 35.0 41.0 24.0 41.0 50-51 34.67641149868778 39.0 35.0 40.5 23.0 41.0 52-53 34.43533162546272 38.5 35.0 40.0 22.5 41.0 54-55 34.20999448804595 38.0 34.0 40.0 21.0 41.0 56-57 33.95786173764351 38.0 34.0 40.0 19.5 41.0 58-59 33.725330090884604 38.0 34.0 40.0 18.5 41.0 60-61 33.38567158149236 37.5 33.5 40.0 16.5 41.0 62-63 33.034160022047814 37.0 33.0 40.0 12.5 41.0 64-65 32.684731887280535 36.0 33.0 39.5 8.0 41.0 66-67 32.24323924386012 36.0 32.0 39.0 6.5 40.5 68-69 31.856008812862896 35.0 32.0 39.0 2.0 40.0 70-71 31.437581035119916 35.0 31.5 38.0 2.0 40.0 72-73 31.006412344271638 35.0 31.0 37.0 2.0 39.5 74-75 30.56783539927217 35.0 31.0 37.0 2.0 39.0 76-77 29.233229253443405 33.5 28.5 35.5 2.0 38.0 78-79 29.838628932747895 34.0 30.0 36.0 2.0 38.0 80-81 29.666312252196953 34.0 30.0 35.5 2.0 37.0 82-83 29.327164224912778 34.0 30.0 35.0 2.0 37.0 84-85 28.955498800523635 34.0 29.5 35.0 2.0 36.5 86-87 28.58914254664804 34.0 29.0 35.0 2.0 36.0 88-89 28.170774272954468 34.0 29.0 35.0 2.0 36.0 90-91 27.711640244780867 34.0 27.5 35.0 2.0 35.0 92-93 27.355593067465065 34.0 27.0 35.0 2.0 35.0 94-95 26.961794485540516 33.0 26.0 35.0 2.0 35.0 96-97 26.50039930348944 33.0 25.0 35.0 2.0 35.0 98-99 26.055640983883798 33.0 24.5 35.0 2.0 35.0 100-101 24.297879620176257 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08953958801086515 1101 2 0.23420887478548735 1101 3 0.16432782480775643 1101 4 0.06534206477992655 1101 5 -0.02097878048670765 1101 6 0.06271963944100634 1101 7 -0.0013273508465871942 1101 8 0.0802016223716393 1101 9 0.10307814912842161 1101 10-11 0.10225606444780766 1101 12-13 0.16014083504986587 1101 14-15 0.17145531516359824 1101 16-17 0.08600720050502986 1101 18-19 0.09429298818825771 1101 20-21 0.15663812760264761 1101 22-23 0.25274385416817324 1101 24-25 0.20457773153743375 1101 26-27 0.18643679250519796 1101 28-29 0.18053241219342198 1101 30-31 0.16558783803971266 1101 32-33 0.02787577858338608 1101 34-35 -0.08523389890640942 1101 36-37 0.035346109383148416 1101 38-39 0.009773946816402201 1101 40-41 -0.09044229611210142 1101 42-43 0.09386560876495054 1101 44-45 0.029881808335808557 1101 46-47 0.27558014368555206 1101 48-49 0.31926699332149155 1101 50-51 0.21550910069935725 1101 52-53 0.2644078521906579 1101 54-55 0.16211384448577348 1101 56-57 0.31383599296059117 1101 58-59 0.2419594724598042 1101 60-61 0.18836650533337718 1101 62-63 0.022707380939735344 1101 64-65 0.311528849656483 1101 66-67 0.5559659544205786 1101 68-69 0.27301476000434377 1101 70-71 -0.05810300328408147 1101 72-73 -0.2844356987718193 1101 74-75 -0.3566999221972402 1101 76-77 0.02381625299819845 1101 78-79 0.1430505435937981 1101 80-81 0.042848797131846084 1101 82-83 0.29544431899761037 1101 84-85 0.16161532170469073 1101 86-87 0.07858177538466649 1101 88-89 -0.08802064287511868 1101 90-91 0.20072708287895935 1101 92-93 0.15228954871951217 1101 94-95 -0.20891924944922025 1101 96-97 -0.16564379500055182 1101 98-99 0.10164380112046345 1101 100-101 0.35866391621202354 1102 1 0.20671278725017572 1102 2 0.20818542257666905 1102 3 0.14897159007674077 1102 4 0.15531896395062006 1102 5 -0.08191226291891951 1102 6 -0.07423052877007308 1102 7 -0.03084560477527276 1102 8 0.05587096474587128 1102 9 0.07197393898447046 1102 10-11 0.16903597978433282 1102 12-13 0.12625289187838007 1102 14-15 0.06390587867662845 1102 16-17 0.02209886307622355 1102 18-19 0.017357492446365086 1102 20-21 0.016461440684182094 1102 22-23 -0.02601525380268299 1102 24-25 0.10239870367161075 1102 26-27 0.13980303205581635 1102 28-29 0.246744897087666 1102 30-31 0.18939498766328455 1102 32-33 0.28203475882303763 1102 34-35 0.34161624485054176 1102 36-37 0.1794888739327476 1102 38-39 0.11712870005538889 1102 40-41 0.4544879859174529 1102 42-43 0.47168050298309794 1102 44-45 0.5023139481658703 1102 46-47 0.6680591974299972 1102 48-49 0.6421752761440231 1102 50-51 0.7369610578595527 1102 52-53 0.4911188686508936 1102 54-55 0.643427813958489 1102 56-57 0.8322715468700466 1102 58-59 0.8667443118579925 1102 60-61 0.6809424258646217 1102 62-63 0.30659473056473274 1102 64-65 0.17625129544788365 1102 66-67 0.4519588033724844 1102 68-69 0.7197736400533472 1102 70-71 0.5727455070329448 1102 72-73 0.5477556511190897 1102 74-75 0.09227407334403992 1102 76-77 -0.04020485826327658 1102 78-79 0.13444530661442755 1102 80-81 0.320806617344239 1102 82-83 0.2453086342705113 1102 84-85 0.0751818950658496 1102 86-87 0.25818063292638627 1102 88-89 -0.08120198082387375 1102 90-91 -0.06480780934486674 1102 92-93 0.09349995620104679 1102 94-95 0.03992392844616077 1102 96-97 0.26778521802661004 1102 98-99 0.35214534790261354 1102 100-101 0.5287707783216788 1103 1 0.26843141578606833 1103 2 0.17427150033199013 1103 3 0.25026979846685293 1103 4 0.20240702628657203 1103 5 0.14122502067490927 1103 6 0.12861053101428155 1103 7 0.10313187892281661 1103 8 0.1724902270671933 1103 9 0.25696520500058995 1103 10-11 0.26584144515477703 1103 12-13 0.18293576138077583 1103 14-15 0.286511578172707 1103 16-17 0.3543937973655673 1103 18-19 0.26789936526858327 1103 20-21 0.3487083609259898 1103 22-23 0.22812424301010026 1103 24-25 0.2564437728575655 1103 26-27 0.03861914282651213 1103 28-29 0.012822743863402764 1103 30-31 0.14022517340905694 1103 32-33 0.28156605026373427 1103 34-35 0.3118441058018675 1103 36-37 0.46433678372121534 1103 38-39 0.3026869891090982 1103 40-41 0.47640411223554935 1103 42-43 0.3099691908734954 1103 44-45 -0.04134225296268568 1103 46-47 -0.0821671545084044 1103 48-49 -0.032449528370214864 1103 50-51 0.15911656850038014 1103 52-53 -0.0037321963464052033 1103 54-55 -0.1926396526374461 1103 56-57 -0.2590911832544265 1103 58-59 -0.05367469073018327 1103 60-61 -0.2550194098267369 1103 62-63 -0.2540595865840558 1103 64-65 -0.2873563429011625 1103 66-67 0.00708121632987968 1103 68-69 0.12441088249228116 1103 70-71 -0.03424007960726172 1103 72-73 0.19663120785340382 1103 74-75 0.2658391392376025 1103 76-77 -0.0497630832982594 1103 78-79 -0.002034623513697653 1103 80-81 -0.3746688589093843 1103 82-83 -0.34510867639409426 1103 84-85 -0.13218620348641252 1103 86-87 -0.07952159766908196 1103 88-89 0.07903118453650748 1103 90-91 0.15395447996491995 1103 92-93 0.39153572438008055 1103 94-95 0.005933412363742008 1103 96-97 -0.02796416693630377 1103 98-99 0.2701487866229684 1103 100-101 0.5080801534074162 1104 1 0.18055174563989596 1104 2 0.10594800038764873 1104 3 0.07077476939484484 1104 4 0.08393752406443156 1104 5 -0.004713033256024346 1104 6 -0.03124911840899358 1104 7 -0.150411138229245 1104 8 0.015685493339383072 1104 9 -0.015236988887011194 1104 10-11 -0.03459784615616002 1104 12-13 0.06861405114177899 1104 14-15 0.14011646432518887 1104 16-17 0.05993910088761112 1104 18-19 0.024499438911959714 1104 20-21 -0.12926177925332638 1104 22-23 -0.16176927380370643 1104 24-25 -0.12161863562340613 1104 26-27 0.041314404817107686 1104 28-29 0.056227379970927416 1104 30-31 0.06930381768585647 1104 32-33 0.13220486374903118 1104 34-35 0.1300766219220577 1104 36-37 0.003358140300740331 1104 38-39 -0.017122223163653416 1104 40-41 -0.22893567488371502 1104 42-43 -0.14124635301710242 1104 44-45 -0.020242568944162542 1104 46-47 0.11807004567314294 1104 48-49 0.508533867617075 1104 50-51 0.499453872296435 1104 52-53 0.3341324882287324 1104 54-55 0.40798738647061583 1104 56-57 0.34310077762895475 1104 58-59 0.3094588778052483 1104 60-61 0.12329240137219699 1104 62-63 0.07227156028310588 1104 64-65 0.22505385071241335 1104 66-67 0.21070729636139163 1104 68-69 -0.14815738748776397 1104 70-71 -0.14723449641165232 1104 72-73 0.003150359413186976 1104 74-75 -0.3087429774100805 1104 76-77 -0.16979215530606595 1104 78-79 -0.026014748364911355 1104 80-81 0.02564605216195659 1104 82-83 0.19613589668839282 1104 84-85 0.06714972163711153 1104 86-87 0.0025982612096555613 1104 88-89 0.00587697872895987 1104 90-91 0.034317173599905715 1104 92-93 0.08046417041918019 1104 94-95 0.12246228313674123 1104 96-97 0.3802524216626715 1104 98-99 0.06076824731367836 1104 100-101 0.27387173408544996 1105 1 -0.04774919847147174 1105 2 4.9888610377024634E-5 1105 3 -0.03334882649792803 1105 4 -0.03306423322314345 1105 5 0.01655962197104799 1105 6 -0.08320970003672556 1105 7 -0.010975968358110322 1105 8 0.036518826672718774 1105 9 0.14162461610230537 1105 10-11 -0.033472622188135404 1105 12-13 -0.0385438807719396 1105 14-15 -0.10801857116513247 1105 16-17 0.00800220818552333 1105 18-19 -0.03430724222648962 1105 20-21 0.018461284284683188 1105 22-23 0.10467837797769874 1105 24-25 0.025190250738660325 1105 26-27 -0.007903645282816285 1105 28-29 0.14477012340693562 1105 30-31 0.17415638642680875 1105 32-33 0.08730358196280719 1105 34-35 -0.06053631826125638 1105 36-37 -0.229894866039281 1105 38-39 -0.16586929435102604 1105 40-41 -0.26565350962976453 1105 42-43 -0.356979245305709 1105 44-45 -0.07723704727094827 1105 46-47 -0.1833532280502439 1105 48-49 -0.3397191817937184 1105 50-51 -0.5415473509135396 1105 52-53 -0.5802062031088724 1105 54-55 -0.7923552415357236 1105 56-57 -0.709781518560149 1105 58-59 -0.8422859192452634 1105 60-61 -0.9679314977388245 1105 62-63 -0.7874404685226324 1105 64-65 -0.8951243761499654 1105 66-67 -0.875341649266062 1105 68-69 -0.7536269450647737 1105 70-71 -0.7198733565866924 1105 72-73 -0.7701067817211289 1105 74-75 -0.7416365581419377 1105 76-77 -0.7911559052199273 1105 78-79 -0.7701118988025151 1105 80-81 -0.9198048126991836 1105 82-83 -0.8555253430607621 1105 84-85 -0.7015279194627446 1105 86-87 -0.4395393843695601 1105 88-89 -0.5101356582361021 1105 90-91 -0.8155219570595946 1105 92-93 -0.9210831471330216 1105 94-95 -0.81495803593155 1105 96-97 -0.8189176045243585 1105 98-99 -0.8991242480346813 1105 100-101 -0.8949603157388211 1106 1 -0.07901076997409717 1106 2 -0.04522245827550364 1106 3 -0.01587585752806575 1106 4 0.04890366279995817 1106 5 -0.06895378245759787 1106 6 -0.18333559798532661 1106 7 0.06368568090501725 1106 8 -0.10251046311473999 1106 9 -0.16947060347852982 1106 10-11 -0.2742397202404234 1106 12-13 -0.42127595882867297 1106 14-15 -0.27437512156133437 1106 16-17 -0.05971191089913219 1106 18-19 0.018780497535047402 1106 20-21 0.03394974055365907 1106 22-23 0.012130657100854592 1106 24-25 -0.06714802594059677 1106 26-27 -0.07070797580195887 1106 28-29 0.025289730160999113 1106 30-31 0.05486692297424156 1106 32-33 -0.10294580706185741 1106 34-35 -0.13990872624466277 1106 36-37 0.05170806479090828 1106 38-39 0.22115349688054664 1106 40-41 0.22626032433305454 1106 42-43 -0.12092059167317615 1106 44-45 0.04438754541216383 1106 46-47 0.3422487215165191 1106 48-49 0.3719004582862695 1106 50-51 0.3488820906920296 1106 52-53 0.5721280004014488 1106 54-55 0.3616788157108104 1106 56-57 0.4691324264891037 1106 58-59 0.47364155983346024 1106 60-61 0.5186825880198995 1106 62-63 0.4462691700761994 1106 64-65 0.28501383387743573 1106 66-67 0.30855498481525245 1106 68-69 0.495574621253823 1106 70-71 0.390291977042871 1106 72-73 0.5675947938546706 1106 74-75 0.5616085540553506 1106 76-77 0.4960661472869248 1106 78-79 0.5759662875264517 1106 80-81 0.5205741771549199 1106 82-83 0.3150779488003046 1106 84-85 0.09731539313445481 1106 86-87 0.4236842974048578 1106 88-89 0.809060770335325 1106 90-91 0.6025899902854412 1106 92-93 0.6319571494677341 1106 94-95 0.09201865443355217 1106 96-97 0.5580842654304661 1106 98-99 0.9696826837582542 1106 100-101 1.2943066527126348 1107 1 -0.0674351378398299 1107 2 -0.040618853036946234 1107 3 -0.08887653979890331 1107 4 0.007147309296371418 1107 5 -0.07599242839119569 1107 6 -0.13898799362890202 1107 7 -0.15843367743878645 1107 8 -0.0758950088762731 1107 9 -0.15495913926098837 1107 10-11 0.043773148719928656 1107 12-13 -0.02558184588413326 1107 14-15 -0.23689157531509153 1107 16-17 -0.04506146558531299 1107 18-19 -0.0511246966824217 1107 20-21 0.06876967388867428 1107 22-23 -0.03444753642055787 1107 24-25 -0.013348558433449398 1107 26-27 -0.1575539319115009 1107 28-29 -0.16024543894798882 1107 30-31 -0.17321713646831682 1107 32-33 -0.23854311565272468 1107 34-35 -0.06620624686784993 1107 36-37 -0.14034428398962007 1107 38-39 -0.2305959420263548 1107 40-41 -0.16090655176242308 1107 42-43 0.013141033234070676 1107 44-45 -0.11607589337068447 1107 46-47 0.17577119165855493 1107 48-49 0.37097927779691275 1107 50-51 0.2155590182220024 1107 52-53 0.25712102837455575 1107 54-55 0.24680232510983302 1107 56-57 0.3115749560828931 1107 58-59 0.19575172934445817 1107 60-61 0.5845584790495266 1107 62-63 0.25957850524117987 1107 64-65 0.2393866845520165 1107 66-67 0.1293072745575259 1107 68-69 0.3186654689466195 1107 70-71 0.3041076168957595 1107 72-73 0.2509694386488519 1107 74-75 0.1988242458251399 1107 76-77 0.3251320335153913 1107 78-79 0.42434939753286116 1107 80-81 0.3789616288848343 1107 82-83 0.25902721725435285 1107 84-85 0.35178633459929287 1107 86-87 0.18485504659337337 1107 88-89 0.4050725871743204 1107 90-91 0.5861879237169418 1107 92-93 0.3793791075620625 1107 94-95 0.22681194015341433 1107 96-97 -0.12544544091740661 1107 98-99 -0.004315216593450799 1107 100-101 0.28830289907443074 1108 1 -0.04044910862420892 1108 2 0.06412221565440035 1108 3 0.013427903061824509 1108 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-0.8833068128530428 2316 82-83 -1.2724107765556454 2316 84-85 -1.0215501327099155 2316 86-87 -1.2855479715685725 2316 88-89 -1.325834221587133 2316 90-91 -1.2611608042786138 2316 92-93 -1.069511632016475 2316 94-95 -1.1030835648183874 2316 96-97 -0.6794910140173975 2316 98-99 -1.102562974090528 2316 100-101 -1.230232500137621 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 4.0 5 14.0 6 37.0 7 104.0 8 204.0 9 412.0 10 801.0 11 1328.0 12 2290.0 13 3214.0 14 4093.0 15 6058.0 16 8740.0 17 12845.0 18 10592.0 19 4876.0 20 3751.0 21 3747.0 22 3851.0 23 4156.0 24 4672.0 25 5279.0 26 6298.0 27 7472.0 28 9237.0 29 11459.0 30 13890.0 31 17394.0 32 22149.0 33 28890.0 34 39963.0 35 59460.0 36 85407.0 37 128779.0 38 114541.0 39 12600.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.528738063343596 19.82671774263041 15.969699106170635 18.674845087855356 2 15.355333128722917 22.73164300075789 39.77642136383282 22.136602506686376 3 19.518267743167996 28.790721126442975 30.729143830682794 20.96186729970624 4 11.944185201656092 17.84933574690109 37.930386525777784 32.276092525665035 5 11.773485733616917 40.22559334935019 32.630063869041834 15.370857047991054 6 28.195054273956643 38.090890869573386 17.576713246854116 16.137341609615856 7 26.302199144394407 36.39972314958065 20.26191177115369 17.036165934871253 8 27.77633367365474 32.51630097774549 19.425253518568393 20.28211183003138 9 28.54628475506254 16.449268100192292 20.60656548890406 34.397881655841104 10-11 24.77842571076021 28.315706563609826 25.663861624899 21.242006100730958 12-13 27.541356350493896 26.36439940118635 27.122781852689336 18.971462395630414 14-15 23.651920101720606 26.077962831891664 28.922726162364626 21.3473909040231 16-17 19.649959599882244 29.923881793639957 28.998985299368 21.427173307109797 18-19 19.52468791691982 26.798666482934863 34.51375796258135 19.162887637563966 20-21 21.57170551132769 24.895319849924526 34.54178750164419 18.991187137103594 22-23 20.581015979348752 25.509019261500253 34.52037290627782 19.389591852873174 24-25 20.33254960175318 25.680245344783177 34.93180125945117 19.05540379401248 26-27 20.111007934507043 25.61677599384907 35.2621268612894 19.01008921035448 28-29 19.498852165851858 26.154724284913417 35.26951396348854 19.076909585746186 30-31 19.612769569002776 25.90046851609428 35.059316141882704 19.42744577302024 32-33 19.543478669364184 26.150776997613573 34.74198730997852 19.563757023043728 34-35 19.99915441614 25.75061226535048 34.88283965850939 19.367393660000126 36-37 20.284538968888775 25.616180090571426 34.66932973386031 19.429951206679487 38-39 20.036422741821326 25.998493608012375 34.66267467570293 19.30240897446337 40-41 19.769907236318765 26.334300013153527 34.41228789938178 19.483504851145923 42-43 19.701564764721496 26.596145368153913 33.535751228798 20.166538638326596 44-45 20.0327428861343 26.33774498443499 33.20294638998328 20.426565739447426 46-47 20.52224198730998 27.028461726369063 32.32408723920002 20.125209047120943 48-49 20.52967999348587 27.317995903615966 31.569090464945855 20.583233637952308 50-51 20.36228570712733 27.690522570825475 31.422052827068704 20.525138894978483 52-53 20.6420330341428 28.321187199739434 30.396077743606448 20.64070202251132 54-55 20.06406080687491 27.920709288268935 30.669091717662685 21.346138187193475 56-57 20.858361571658534 27.999708743337116 30.070997726318954 21.0709319586854 58-59 21.174124507525697 27.36544255353798 30.41588632847488 21.04454661046144 60-61 20.764016335427456 27.806085698358313 31.27243146073046 20.157466505483768 62-63 21.00751316918567 27.714793959399447 31.081157259807206 20.196535611607676 64-65 20.563346758282027 28.145102190375376 30.951109593931843 20.34044145741076 66-67 20.75266358915899 28.029460768040686 30.34518612240296 20.872689520397362 68-69 20.764251219833014 28.031339843285124 30.487839251376425 20.71656968550544 70-71 20.892341515662093 28.37528890781883 30.133555272998315 20.59881430352076 72-73 20.724418227741857 28.054020535257095 30.331157249584646 20.890403987416402 74-75 20.321337525758988 29.037428047077096 29.873616530851283 20.76761789631263 76-77 20.332063913612647 28.323692633398682 30.424890230687808 20.919353222300867 78-79 20.237875266985274 28.254010259750835 30.47993147638942 21.028182996874474 80-81 20.199823993285438 28.93791535392382 30.045786800122766 20.816473852667976 82-83 20.179984513251817 28.49240656080663 30.069502458854025 21.25810646708753 84-85 20.040400718756974 28.235404653129294 29.877740848180206 21.846453779933526 86-87 20.11111598278767 28.205624072206597 29.712564123442714 21.970695821563016 88-89 20.107592716704353 27.891818506385725 29.741220020920373 22.259368755989552 90-91 19.97815362931642 27.678412027249237 30.009631195677706 22.333803147756637 92-93 20.058424038571452 27.914272784357202 30.12376939181688 21.903533785254467 94-95 19.61151685217315 27.631018521418326 30.280827795281017 22.476636831127507 96-97 19.429716322273933 27.502145277570733 30.424420461876693 22.643717938278645 98-99 19.588842033726323 27.575628982275596 29.918604596538422 22.91692438745966 100-101 19.27410084786374 27.343214114566532 30.48206393901144 22.90062109855829 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1188.0 1 860.5 2 880.5 3 1162.0 4 854.5 5 728.5 6 1375.5 7 3009.0 8 4427.5 9 4700.5 10 3803.5 11 2758.5 12 2579.0 13 2579.0 14 2583.0 15 2552.5 16 2450.5 17 2422.5 18 2500.0 19 2589.0 20 2679.5 21 2848.5 22 3062.5 23 3342.0 24 3783.5 25 4232.0 26 4778.0 27 5519.5 28 6227.0 29 7285.0 30 8506.5 31 9376.5 32 10707.5 33 12140.0 34 13480.5 35 14717.0 36 16632.0 37 18524.0 38 18996.5 39 18931.0 40 18945.0 41 19572.0 42 19938.5 43 20315.5 44 21071.0 45 21621.5 46 21310.0 47 23186.5 48 29907.5 49 28952.5 50 22171.0 51 18630.0 52 16077.5 53 15228.5 54 14514.0 55 13453.5 56 12243.5 57 11061.0 58 10392.0 59 9724.5 60 8751.5 61 7944.0 62 6628.5 63 4620.0 64 3033.5 65 2216.5 66 1617.5 67 1239.0 68 824.0 69 479.0 70 369.5 71 316.0 72 267.0 73 245.5 74 196.0 75 146.0 76 98.5 77 45.5 78 31.0 79 20.5 80 9.0 81 8.0 82 6.5 83 1.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054336592484951735 2 0.0 3 0.0 4 0.0 5 0.018477573236957652 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0025054336592484953 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010178324240697012 24-25 7.829480185151548E-5 26-27 0.0014093064333272785 28-29 0.002818612866654557 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012762052701797023 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.697688111090929E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3488440555454644E-4 84-85 0.001487601235178794 86-87 0.0 88-89 0.0 90-91 0.009708555429587918 92-93 7.829480185151547E-4 94-95 0.0 96-97 0.0 98-99 1.5658960370303095E-4 100-101 0.010334913844400042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 638612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.295208978381304 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34713277985767 39.67537339821406 2 12.654304285593698 12.9821036561111 3 4.0910787746748785 6.295582220819041 4 1.8532471079395936 3.8025079076136894 5 1.0250848045700878 2.629096963549291 6 0.6108193371825923 1.8799263329290457 7 0.4108474076226494 1.4752152542561008 8 0.2948866445437995 1.210101764544627 9 0.2434200234070023 1.1237652873166188 >10 1.2982253819919982 12.366439435931852 >50 0.11141132327113874 3.933024916909099 >100 0.05156225634001055 5.025618625935935 >500 0.004296854695000539 1.6255094390322122 >1k 0.002762263732500346 2.2369843698991483 >5k 9.207545775001154E-4 3.738750426938206 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9994 1.5649564994080913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7505 1.1752049757912473 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6249 0.9785284335402403 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3010 0.47133470714612313 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1753 0.2745015752914132 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.268081401539589 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1625 0.2544581060174253 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1415 0.22157428923978878 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1409 0.2206347516175706 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1218 0.1907261373102917 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1059 0.16582839032150978 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.1578423205326552 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 954 0.14938648193269152 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 947 0.1482903547067703 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 886 0.13873838888088544 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.1340407007697945 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 854 0.13372752156238843 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.13247480473276418 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 809 0.12668098939575204 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 729 0.11415382109950956 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 691 0.10820341615879439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.5658960370303095E-4 0.0 24-25 0.0 0.0 0.0 4.6976881110909283E-4 0.0 26-27 0.0 0.0 0.0 5.480636129606084E-4 0.0 28-29 0.0 0.0 0.0 8.612428203666702E-4 0.0 30-31 0.0 0.0 0.0 0.0021139596499909178 0.0 32-33 0.0 0.0 0.0 0.004619393309239413 0.0 34-35 0.0 0.0 0.0 0.005715520535160629 0.0 36-37 0.0 0.0 0.0 0.007594595779597001 0.0 38-39 0.0 0.0 0.0 0.01056979824995459 0.0 40-41 7.829480185151548E-5 0.0 0.0 0.016285318785115216 0.0 42-43 1.5658960370303095E-4 0.0 0.0 0.022783787338791002 0.0 44-45 3.131792074060619E-4 0.0 0.0 0.034762892022072875 0.0 46-47 3.131792074060619E-4 0.0 0.0 0.06247925187750935 0.0 48-49 3.131792074060619E-4 0.0 0.0 0.08722040926258824 0.0 50-51 3.131792074060619E-4 0.0 0.0 0.12167012207725504 0.0 52-53 3.131792074060619E-4 0.0 0.0 0.1664547487363219 0.0 54-55 3.131792074060619E-4 0.0 0.0 0.20607191847318873 0.0 56-57 3.131792074060619E-4 0.0 0.0 0.2555542332433465 0.0 58-59 3.131792074060619E-4 0.0 0.0 0.3349451623207832 0.0 60-61 3.131792074060619E-4 0.0 0.0 0.44142609283884426 0.0 62-63 3.131792074060619E-4 0.0 0.0 0.5731962443549448 0.0 64-65 4.6976881110909283E-4 0.0 0.0 0.6829655565507695 0.0 66-67 4.6976881110909283E-4 0.0 0.0 0.8451923859871096 0.0 68-69 4.6976881110909283E-4 0.0 0.0 1.0439828878881072 0.0 70-71 5.480636129606084E-4 0.0 0.0 1.203391104457793 0.0 72-73 7.046532166636393E-4 0.0 0.0 1.4474360018289665 0.0 74-75 9.395376222181857E-4 0.0 0.0 1.6596932096484251 0.0 76-77 0.0010961272259212165 0.0 0.0 1.85730928952165 0.0 78-79 0.0010961272259212165 0.0 0.0 2.104564273768736 0.0 80-81 0.001644190838881825 0.0 0.0 2.3585526109750523 0.0 82-83 0.0017224856407333405 0.0 0.0 2.6927931200791715 0.0 84-85 0.0017224856407333405 0.0 0.0 3.090139239475613 0.0 86-87 0.0017224856407333405 0.0 0.0 3.5885639480623603 0.0 88-89 0.0017224856407333405 0.0 0.0 4.1621516664265625 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 105 4.6209607E-8 36.217968 1 TACCTGG 510 0.0 28.874119 2 GTATAGA 165 8.3364284E-8 25.928774 1 GTATCAA 11700 0.0 25.068174 1 CATATAG 175 1.483204E-7 24.4299 3 AGTACTT 6835 0.0 24.010033 12-13 GTATTAG 205 2.6431735E-8 23.188334 1 GAGTACT 6665 0.0 23.090221 12-13 GTACATG 6925 0.0 22.995794 1 TACATGG 7100 0.0 22.27938 2 GGTATCA 8590 0.0 22.080206 1 GTATAGG 260 6.184564E-10 21.93973 1 ATGGGAG 1660 0.0 21.74817 5 ACATGGG 7050 0.0 21.696215 3 TGTATCG 110 0.0028763795 21.590393 9 GTACTTT 7205 0.0 21.293732 14-15 TACTTTT 7605 0.0 21.20429 14-15 CATGGGG 1470 0.0 21.004543 4 TATCAAC 14005 0.0 20.893677 2 ATCAACG 14045 0.0 20.766527 3 >>END_MODULE