FastQCFastQC Report
Thu 26 May 2016
SRR937268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671890
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81051.2062986500766493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57840.8608551995118249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.6507017517748441No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28760.428046257571924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20460.30451413177752307No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16640.24765958713479885No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13800.20539076336900386No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.19422822188155797No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11750.17487981663665184No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10360.15419190641325217No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9860.14675021208828826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.14392236824480198No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9310.13856434833082798No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9040.13454583339534745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.12055544806441532No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7870.11713226867493191No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7590.11296491985295211No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7260.10805340159847594No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7140.1062673949604846No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7110.10582089330098676No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6840.10180237836550626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA550.002576260434.5525679
CGTCACT1303.02427E-729.2411481
TACCTGG3950.026.4591662
GTACATG77850.025.818411
GTATCAA111450.025.6663551
TACATGG78650.025.3084682
GAGTACT54500.024.88257812-13
AGTACTT55500.024.81937212-13
ACATGGG78800.024.2993243
ATGGGTA2402.2737368E-1023.7602065
CATGGGG16800.022.9116274
GTACTTT59300.022.90596214-15
GTATTGC1252.2491273E-422.8080941
ATGGGAG19400.022.7804055
TACTTTT62700.022.11833814-15
ATGGGAT19450.021.988885
GTCTAGG1302.9326888E-421.930861
CATGGGA61600.021.7544754
ATCAACG132300.021.6916433
GTACTAG1100.00287020921.5985761
Produced by FastQC (version 0.11.5)