##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937268_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 671890 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.849274434803316 33.0 31.0 34.0 30.0 34.0 2 31.8823349060114 33.0 31.0 34.0 30.0 34.0 3 31.906733245025226 33.0 31.0 34.0 30.0 34.0 4 35.55075682031285 37.0 35.0 37.0 33.0 37.0 5 35.59298843560702 37.0 35.0 37.0 33.0 37.0 6 35.66999360014288 37.0 35.0 37.0 33.0 37.0 7 35.56826117370403 37.0 35.0 37.0 33.0 37.0 8 35.58891336379467 37.0 35.0 37.0 33.0 37.0 9 37.19064132521692 39.0 37.0 39.0 34.0 39.0 10-11 37.085451487594696 39.0 37.0 39.0 33.0 39.0 12-13 36.94144353986516 39.0 37.0 39.0 33.0 39.0 14-15 38.01696036553602 40.0 38.0 41.0 33.0 41.0 16-17 38.00069654258881 40.0 38.0 41.0 33.0 41.0 18-19 38.06908273675751 40.0 38.0 41.0 33.0 41.0 20-21 38.021443242197385 40.0 38.0 41.0 33.0 41.0 22-23 37.8700055068538 40.0 37.5 41.0 33.0 41.0 24-25 37.7933932637783 40.0 37.0 41.0 33.0 41.0 26-27 37.78443048713331 40.0 37.0 41.0 33.0 41.0 28-29 37.69419399008766 40.0 37.0 41.0 33.0 41.0 30-31 37.40080519132596 39.5 36.5 41.0 32.5 41.0 32-33 37.075921653842144 39.0 35.5 41.0 32.0 41.0 34-35 37.005544062272094 39.0 35.0 41.0 32.0 41.0 36-37 36.82911041986039 39.0 35.0 41.0 31.0 41.0 38-39 36.67584798106833 39.0 35.0 41.0 31.0 41.0 40-41 36.41524803167185 39.0 35.0 41.0 30.5 41.0 42-43 36.04459658575064 39.0 35.0 41.0 30.0 41.0 44-45 35.62661075473664 39.0 35.0 41.0 28.0 41.0 46-47 35.055968983018055 39.0 35.0 40.5 25.0 41.0 48-49 34.56054785753621 38.0 35.0 40.0 23.0 41.0 50-51 33.52564184613553 37.5 33.5 39.5 21.0 40.5 52-53 33.61759142121478 38.0 33.5 39.5 20.0 40.5 54-55 33.972620518239594 38.0 34.0 40.0 19.5 41.0 56-57 33.76942728720475 38.0 34.0 40.0 18.0 41.0 58-59 33.510643855393 38.0 33.5 40.0 16.0 41.0 60-61 33.18822128622244 37.0 33.0 40.0 12.5 41.0 62-63 32.87959785083868 37.0 33.0 40.0 8.5 41.0 64-65 32.46853577222462 36.0 32.5 39.5 7.0 41.0 66-67 32.09994939647859 36.0 32.0 39.0 3.5 41.0 68-69 31.640970248106086 35.0 31.0 39.0 2.0 40.0 70-71 31.174344014645257 35.0 31.0 38.0 2.0 40.0 72-73 30.72295762699251 35.0 30.5 37.5 2.0 39.5 74-75 30.24713792436262 35.0 30.0 37.0 2.0 39.0 76-77 29.777795472473173 34.5 29.5 36.0 2.0 39.0 78-79 29.2903086814806 34.0 29.0 36.0 2.0 38.5 80-81 28.801767402402177 34.0 29.0 35.5 2.0 37.0 82-83 28.32526901724985 34.0 28.0 35.0 2.0 37.0 84-85 27.80587000848353 34.0 26.5 35.0 2.0 36.0 86-87 27.36011103007933 33.5 26.0 35.0 2.0 36.0 88-89 26.893788417746954 33.0 25.5 35.0 2.0 35.5 90-91 26.37415201893167 33.0 24.5 35.0 2.0 35.0 92-93 25.898717051898377 33.0 23.5 35.0 2.0 35.0 94-95 25.38700903421691 32.0 19.5 35.0 2.0 35.0 96-97 24.68286549881677 32.0 12.5 34.5 2.0 35.0 98-99 23.820927532780665 31.5 2.0 34.0 2.0 35.0 100-101 21.429450505291044 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0015200952901857079 1101 2 0.1647718172697843 1101 3 0.033199348397097594 1101 4 0.07628416294051021 1101 5 -0.039426810283522684 1101 6 -0.030727588643884474 1101 7 -0.0706553552861493 1101 8 -0.05068572121113135 1101 9 0.07169113824149065 1101 10-11 -0.06302690175844106 1101 12-13 0.10833878711746081 1101 14-15 0.10410600708095075 1101 16-17 0.16009629318472207 1101 18-19 0.28129243663162384 1101 20-21 0.4789683632156141 1101 22-23 0.21063876168443585 1101 24-25 0.11184730381739882 1101 26-27 0.09017885364269773 1101 28-29 0.15497175558874687 1101 30-31 0.3934922673723946 1101 32-33 0.18203537078201038 1101 34-35 0.35862379418885837 1101 36-37 0.35788914164834296 1101 38-39 0.24165721613854885 1101 40-41 0.2805444132416639 1101 42-43 0.17758287240514647 1101 44-45 0.3960178860366952 1101 46-47 0.33868546310812064 1101 48-49 0.2817476118794744 1101 50-51 0.355888627958592 1101 52-53 0.4505373666198693 1101 54-55 0.34584199091309387 1101 56-57 0.3485809458739837 1101 58-59 0.2789812914436496 1101 60-61 0.3604039909646275 1101 62-63 0.44120915177303743 1101 64-65 0.5016771499389137 1101 66-67 0.4733659810709483 1101 68-69 0.6271329641487 1101 70-71 0.6217699876031624 1101 72-73 0.49387334989698317 1101 74-75 0.534060202580509 1101 76-77 0.9199260780959087 1101 78-79 0.6358098728566866 1101 80-81 0.595873153384904 1101 82-83 0.10891369322005673 1101 84-85 0.18921892419264452 1101 86-87 0.3481253525811532 1101 88-89 0.3010971982921795 1101 90-91 0.024056391291342294 1101 92-93 0.2961750850690379 1101 94-95 0.41465364903138635 1101 96-97 0.029260847418129288 1101 98-99 -0.12062873225317006 1101 100-101 0.02502837562103366 1102 1 0.08049123615457177 1102 2 -0.02977694841212397 1102 3 0.010912824722471015 1102 4 -0.07642728991258707 1102 5 0.04784554908515304 1102 6 -0.15247182174788776 1102 7 -0.09058251028412911 1102 8 0.0749152500555681 1102 9 -0.1712267087840047 1102 10-11 -0.06964365610890155 1102 12-13 -0.1492897033722187 1102 14-15 -0.03074830291500774 1102 16-17 -0.19322220135190094 1102 18-19 -0.08142508663831194 1102 20-21 -0.194647581745933 1102 22-23 -0.2228213759116784 1102 24-25 -0.28193051892642984 1102 26-27 -0.4632367675632878 1102 28-29 -0.4203560469203538 1102 30-31 -0.24573071927269297 1102 32-33 -0.4348383645478151 1102 34-35 -0.3286870111531144 1102 36-37 -0.2501946301039837 1102 38-39 0.016410352520580318 1102 40-41 0.08673623841365696 1102 42-43 -0.017763141354457446 1102 44-45 0.3270599742600879 1102 46-47 0.12047825954680746 1102 48-49 0.2848920878001522 1102 50-51 0.10252561703183716 1102 52-53 0.22669681623541038 1102 54-55 0.23052228229310145 1102 56-57 0.2200580806372372 1102 58-59 0.13257902515469056 1102 60-61 0.4540381471766821 1102 62-63 0.34358268475563136 1102 64-65 0.07631292492879993 1102 66-67 0.1899807119491399 1102 68-69 0.21805566750766303 1102 70-71 0.2530123995821185 1102 72-73 0.12793850570434628 1102 74-75 -0.06816561692576428 1102 76-77 0.018036154987854047 1102 78-79 -0.03066119148973101 1102 80-81 0.062050814250948605 1102 82-83 0.11381859002297645 1102 84-85 0.19561107312020454 1102 86-87 -0.1359539675321635 1102 88-89 -0.31933380130312017 1102 90-91 -0.36458181186527483 1102 92-93 0.05776148733532693 1102 94-95 -0.03803756909892542 1102 96-97 -0.014557444771266859 1102 98-99 0.062363983246630283 1102 100-101 0.16934848893547638 1103 1 -0.04723438100447197 1103 2 -0.06094246844449813 1103 3 -0.11108636588861742 1103 4 -0.11085869420234928 1103 5 -0.0022839531406688707 1103 6 -0.1407275886438839 1103 7 -0.09494106957186688 1103 8 -0.0464000069254169 1103 9 -0.07259457604422437 1103 10-11 -0.03659833032986626 1103 12-13 -0.16808978431110688 1103 14-15 -0.3280368500619133 1103 16-17 -0.2027608496724227 1103 18-19 -0.21227899193980448 1103 20-21 -0.2081744939272383 1103 22-23 -0.4172183811727095 1103 24-25 -0.18886698189688644 1103 26-27 -0.024106860643016148 1103 28-29 -0.16002824441125085 1103 30-31 -0.5050791611990277 1103 32-33 -0.4243932006465627 1103 34-35 -0.3806619200968555 1103 36-37 -0.4171108583516556 1103 38-39 -0.5726284981471608 1103 40-41 -0.5137413010440568 1103 42-43 -0.5767028418805609 1103 44-45 -0.6039821139633048 1103 46-47 -0.3906002511775952 1103 48-49 -0.5053952452633865 1103 50-51 -0.48696851489855675 1103 52-53 -0.2908912048087018 1103 54-55 -0.45058658051546985 1103 56-57 -0.5778476255545897 1103 58-59 -0.7417329942706417 1103 60-61 -0.6295960090353745 1103 62-63 -0.36236227679839317 1103 64-65 -0.44475142148965574 1103 66-67 -0.6380625903576203 1103 68-69 -0.3107241787084476 1103 70-71 -0.26608715525398097 1103 72-73 -0.38398379296016216 1103 74-75 -0.32165408313377597 1103 76-77 -0.34650249333266103 1103 78-79 -0.32847584142902875 1103 80-81 -0.3112697037579544 1103 82-83 -0.15894344963708207 1103 84-85 -0.2886382186644987 1103 86-87 -0.0833032188474192 1103 88-89 0.11038291257789723 1103 90-91 0.3490563912913416 1103 92-93 0.29760365649761056 1103 94-95 -0.19248920811147485 1103 96-97 -0.08145343829615825 1103 98-99 0.14722841060397585 1103 100-101 0.6464569470496038 1104 1 0.04445771330897941 1104 2 0.020208710473671232 1104 3 0.034489223570467686 1104 4 -0.1627588328986036 1104 5 0.005164035763634445 1104 6 -0.029104842458032465 1104 7 -0.032846756118331655 1104 8 -0.08208655338866322 1104 9 -0.010486392965169955 1104 10-11 0.03808266828317386 1104 12-13 0.11548164426031349 1104 14-15 0.1963736908534841 1104 16-17 0.049416681534239615 1104 18-19 -0.09148842328516338 1104 20-21 0.02993230218925902 1104 22-23 -0.1004985476082183 1104 24-25 0.0018958475067165637 1104 26-27 0.027432667789710763 1104 28-29 -0.22454974233080804 1104 30-31 -0.3398919212545124 1104 32-33 -0.1463904267214602 1104 34-35 -0.039912960318773116 1104 36-37 0.14766029282726123 1104 38-39 -0.12011116111526121 1104 40-41 0.0653571732971372 1104 42-43 0.08819313592803013 1104 44-45 0.09692634650826193 1104 46-47 0.1210155601954952 1104 48-49 0.38510406125533336 1104 50-51 0.3429760065993648 1104 52-53 0.3619659380484421 1104 54-55 0.22419844028896563 1104 56-57 0.20838677111670023 1104 58-59 0.47106173527166817 1104 60-61 0.3751335332669825 1104 62-63 0.3077348105802429 1104 64-65 0.5664136270540325 1104 66-67 0.4622772154676156 1104 68-69 0.38742422628462236 1104 70-71 0.3284967559804137 1104 72-73 0.31186918900932525 1104 74-75 0.43718086832253533 1104 76-77 0.4278040253913282 1104 78-79 0.2206226329121641 1104 80-81 0.26393834062485055 1104 82-83 0.20339358226305748 1104 84-85 0.23019673279181063 1104 86-87 0.2698867950222059 1104 88-89 0.31484893199537467 1104 90-91 0.15328662707497287 1104 92-93 0.49434429450038664 1104 94-95 0.29905032032126044 1104 96-97 0.24836625657208344 1104 98-99 0.3841216144874693 1104 100-101 0.8909992494074395 1105 1 0.11886072276340798 1105 2 0.06106639716098172 1105 3 0.0055527636701171446 1105 4 0.36863757821353005 1105 5 0.2538333146409144 1105 6 0.22043299970993502 1105 7 0.14147037511884264 1105 8 0.06075493967946244 1105 9 0.03325672357293996 1105 10-11 0.07342282622866492 1105 12-13 0.11175406013781242 1105 14-15 0.19993010540856915 1105 16-17 0.07770660952564867 1105 18-19 0.1323250781790719 1105 20-21 0.12902578816020593 1105 22-23 0.18061861258106404 1105 24-25 0.16013966730722018 1105 26-27 0.23571179742670267 1105 28-29 0.2251097769468018 1105 30-31 0.369250881114084 1105 32-33 0.3198784092667992 1105 34-35 0.31227380426340545 1105 36-37 0.22324779568823772 1105 38-39 0.28568199953569007 1105 40-41 0.4053781831389003 1105 42-43 0.3473692919094802 1105 44-45 0.2524686214789753 1105 46-47 0.2768639841639313 1105 48-49 0.21415945955224913 1105 50-51 0.1766764579001574 1105 52-53 0.04884887175789032 1105 54-55 0.24376361090100573 1105 56-57 0.2680369200831265 1105 58-59 0.19394603051275539 1105 60-61 0.30949929622354233 1105 62-63 0.36943703813209083 1105 64-65 0.4498989915669682 1105 66-67 0.4361939077282102 1105 68-69 0.42937152953253843 1105 70-71 0.057927553591476055 1105 72-73 0.22162370250628882 1105 74-75 0.2505522436846803 1105 76-77 0.1824769545819045 1105 78-79 0.4111372957863644 1105 80-81 0.5019833689803086 1105 82-83 0.4773118395025513 1105 84-85 0.7562418941704792 1105 86-87 0.8306500352327753 1105 88-89 0.49850703105462557 1105 90-91 0.6269226012449955 1105 92-93 0.6262456069308158 1105 94-95 0.6676155672260222 1105 96-97 0.8569709018207092 1105 98-99 0.711584646751465 1105 100-101 0.6266997437451494 1106 1 -0.01888411202659057 1106 2 -0.093241731245854 1106 3 -0.11079945614564224 1106 4 0.11839414500865075 1106 5 -0.06954237115222384 1106 6 -0.029605851245996462 1106 7 -0.29617039812337254 1106 8 -0.1963242946321273 1106 9 -0.40006618393424276 1106 10-11 -0.608334732003506 1106 12-13 -0.3129901628725804 1106 14-15 -0.3310454175056279 1106 16-17 -0.04237431849091422 1106 18-19 -0.12580559086389798 1106 20-21 -0.17613125822291664 1106 22-23 -0.13870772167878442 1106 24-25 -0.17978947641771015 1106 26-27 -0.2456171216511862 1106 28-29 -0.09144486126719897 1106 30-31 -0.3186077525518911 1106 32-33 -0.25141850449194436 1106 34-35 -0.15581234846904835 1106 36-37 -0.27558864277086315 1106 38-39 0.08105450643542156 1106 40-41 0.1476793006694308 1106 42-43 0.018577094361717172 1106 44-45 -0.06077729228567108 1106 46-47 -0.073081821211467 1106 48-49 -0.05989813428510615 1106 50-51 -0.09282426305556157 1106 52-53 0.03700080555192642 1106 54-55 -0.12058458808670736 1106 56-57 -0.09087312864284769 1106 58-59 -0.12725301817978618 1106 60-61 -0.2408751483061451 1106 62-63 -0.20307556629829548 1106 64-65 -0.18305586460581935 1106 66-67 -0.1990398766696373 1106 68-69 -0.1401254538628578 1106 70-71 0.32996883199447424 1106 72-73 -0.015645478554901615 1106 74-75 0.16204386466458942 1106 76-77 0.33276130423657335 1106 78-79 0.15092244508223018 1106 80-81 0.06904011891588269 1106 82-83 0.20558235231549915 1106 84-85 0.22901868510119172 1106 86-87 0.37522437629105454 1106 88-89 0.2419589237354991 1106 90-91 0.5327882103788077 1106 92-93 0.6131346387649934 1106 94-95 0.6385727564232937 1106 96-97 0.7892777830626798 1106 98-99 0.8700217957404561 1106 100-101 0.9219670088148959 1107 1 -0.15847780333929862 1107 2 -0.2607846044803601 1107 3 0.04065013771691639 1107 4 -0.12832507502479729 1107 5 0.030666738732264776 1107 6 -0.2159020185795697 1107 7 -0.15916441784705881 1107 8 -0.1970587479115764 1107 9 -0.23697189040789368 1107 10-11 -0.15981115434077964 1107 12-13 -0.046669983102773926 1107 14-15 -0.09901716578983866 1107 16-17 -0.24602337159797116 1107 18-19 -0.1899656027369261 1107 20-21 -0.1750571678699444 1107 22-23 -0.06545361796865024 1107 24-25 -0.13888842021300718 1107 26-27 -0.15629161994920082 1107 28-29 -0.22838139194583817 1107 30-31 -0.3170846182249534 1107 32-33 -0.2733817019133724 1107 34-35 -0.15069115416428502 1107 36-37 -0.2671985605539575 1107 38-39 -0.21452773806140613 1107 40-41 -0.3433398198513018 1107 42-43 -0.27845883486438794 1107 44-45 -0.31409612682629273 1107 46-47 -0.25278598495268767 1107 48-49 -0.07437789971670838 1107 50-51 -0.2021721789016766 1107 52-53 0.12117466928991405 1107 54-55 -0.2067315814899402 1107 56-57 -0.2012884752914843 1107 58-59 -0.40141434293561673 1107 60-61 -0.2639733233990498 1107 62-63 -0.1966947655919995 1107 64-65 -0.2328921348009061 1107 66-67 -0.27170223175306063 1107 68-69 -0.3857553617137057 1107 70-71 -0.032576143755246534 1107 72-73 -0.018302639188966907 1107 74-75 -0.1169532450905102 1107 76-77 -0.5504539647807256 1107 78-79 -0.21399523336042492 1107 80-81 -0.2541706977162441 1107 82-83 -0.08884113040106811 1107 84-85 0.10746000780207865 1107 86-87 0.031031218884017164 1107 88-89 0.08877308993124089 1107 90-91 0.42503573255035576 1107 92-93 0.5823142392300227 1107 94-95 0.872975613560719 1107 96-97 0.9359505765157721 1107 98-99 1.186752869773727 1107 100-101 1.1889369704368562 1108 1 -0.07842226920323014 1108 2 -0.06499526347555573 1108 3 0.004115497465420503 1108 4 -0.07845186190421316 1108 5 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94-95 -0.3604399839274137 2316 96-97 -0.5180773013245137 2316 98-99 -0.12313006986686759 2316 100-101 -0.19461581913552095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2334.0 3 465.0 4 327.0 5 364.0 6 450.0 7 518.0 8 687.0 9 886.0 10 1224.0 11 1759.0 12 2794.0 13 4117.0 14 4394.0 15 6229.0 16 8606.0 17 12372.0 18 9726.0 19 5150.0 20 4874.0 21 4946.0 22 5502.0 23 5735.0 24 6616.0 25 7314.0 26 8468.0 27 9770.0 28 11914.0 29 14171.0 30 17062.0 31 20718.0 32 26286.0 33 34225.0 34 46339.0 35 67013.0 36 90254.0 37 120732.0 38 96176.0 39 11372.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.166815372725026 19.403753360806654 15.19845544637182 19.230975820096504 2 14.905255369707673 22.129057953611454 40.6805980479757 22.285088628705168 3 19.42095261959949 29.09242504243444 30.000924757549452 21.48569758041662 4 11.630730677724658 17.31654746011739 38.24956556111604 32.80315630104191 5 11.875480113064295 40.33128733694801 32.53119336530358 15.262039184684115 6 28.231730970071318 37.566168352579595 18.30394175394623 15.898158923402859 7 26.841797440327525 36.220350261547324 20.203896503054118 16.733955795071033 8 27.395733723988442 32.81338809267715 19.603268255642394 20.18760992769201 9 28.791056665582666 16.88018569293239 20.430101110756407 33.89865653072854 10-11 24.718429785910413 28.36729929054525 25.721781252820552 21.192489670723784 12-13 27.474639878441494 26.34267474968092 26.890866626535054 19.291818745342532 14-15 23.82848567929558 26.265265299607844 28.172653950742326 21.733595070354255 16-17 20.08955644719146 30.47723904171767 28.009327118238232 21.423877392852642 18-19 20.042752701132365 27.737343872833836 32.84969278022337 19.370210645810428 20-21 22.301791807282832 25.84035948017079 32.478511364271995 19.379337348274383 22-23 21.18707648891693 26.553650827743695 32.3808202709576 19.878452412381776 24-25 20.755977714124207 26.78958550687242 32.808502101885324 19.64593467711805 26-27 20.539767705674006 26.57601922435801 33.21728312744863 19.66692994251935 28-29 19.906742971036763 27.259815783427673 33.172036914924405 19.66140433061116 30-31 19.9697742993523 27.065130715712066 33.008673105168576 19.956421879767056 32-33 19.782557614772657 27.440483072688416 32.60419891316105 20.172760399377886 34-35 20.479227745078294 27.050406940745546 32.64477914792352 19.82558616625264 36-37 20.538973155753904 26.81926186899912 32.61254461735352 20.029220357893447 38-39 20.355966901715696 27.251716255672687 32.532600467612085 19.859716374999532 40-41 20.09826184813712 27.51555477001761 32.426633614411735 19.959549767433536 42-43 19.940201620030443 27.75903743849933 31.52369523857823 20.777065702891996 44-45 20.296815148836497 27.69906718435883 31.175138840712684 20.828978826091983 46-47 20.84677067410877 28.249337364722066 30.523446434526115 20.380445526643047 48-49 20.73438298475506 28.321415649280652 29.985639097463903 20.958562268500387 50-51 20.52097337091376 28.76432206765969 29.91575737048404 20.7989471909425 52-53 20.757166713676188 29.26477106362403 29.05919767429713 20.918864548402645 54-55 20.226046022373335 28.938740035883875 29.353679962101854 21.481533979640936 56-57 20.96800897323839 29.09896217099866 28.67095838839684 21.262070467366108 58-59 21.29847663502621 28.451953978420864 29.071664516706306 21.17790486984662 60-61 20.990566917027493 28.750286139087084 29.810137861252926 20.449009082632493 62-63 21.003976802853337 28.846473635504726 29.670916394673636 20.4786331669683 64-65 20.657796136883036 29.26380070760432 29.585317023886304 20.493086131626335 66-67 20.79137489943025 29.04976403856808 29.028065578562217 21.130795483439453 68-69 20.731989220451315 29.107242192358573 29.30790465438482 20.852863932805292 70-71 20.978217426059153 29.207454931577125 28.95042175215052 20.863905890213204 72-73 20.83896956509419 28.966403335953217 29.077437723056825 21.11718937589577 74-75 20.308109672069534 30.052586993742057 28.697196752313168 20.942106581875237 76-77 20.317001699715025 29.41409428458704 29.043423654156097 21.22548036154184 78-79 20.38296409376028 29.354266396899416 29.107717227641842 21.15505228169846 80-81 20.435931337346783 29.99982848505322 28.577522145935358 20.98671803166464 82-83 20.31577055666622 29.519170611996536 28.763859610137526 21.40119922119971 84-85 20.329717447896275 29.29329536243491 28.58085631385498 21.796130875813834 86-87 20.433308624261524 29.444538077077386 28.527493441158352 21.594659857502734 88-89 20.53753666192134 28.926172044245618 28.41355765867816 22.122733635154876 90-91 20.283034698786196 28.89541886525087 28.724031846064925 22.097514589898008 92-93 20.472968236615365 29.115466472977186 28.84849714163842 21.563068148769034 94-95 20.153284361281607 28.743427660832626 29.02395683340579 22.07933114447998 96-97 20.03898914598258 28.7753768776764 29.139389925765503 22.046244050575513 98-99 20.216560186518016 28.96906432216423 28.547295533502094 22.267079957815664 100-101 20.334390404488165 28.372853577625385 29.283580153110034 22.009175864776413 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1902.0 1 1210.0 2 667.5 3 854.5 4 774.0 5 800.5 6 1287.0 7 2456.5 8 3698.5 9 4010.0 10 3369.0 11 2713.5 12 2578.0 13 2592.5 14 2622.0 15 2566.5 16 2454.0 17 2470.0 18 2505.0 19 2486.5 20 2586.0 21 2774.5 22 3038.5 23 3346.0 24 3721.5 25 4300.5 26 5035.5 27 5780.0 28 6535.5 29 7645.0 30 8995.5 31 9972.0 32 11373.0 33 13079.0 34 14571.0 35 15853.0 36 17747.5 37 19651.5 38 20176.5 39 19957.5 40 19964.0 41 20434.0 42 20665.0 43 21352.0 44 22264.5 45 22830.0 46 22679.0 47 24445.5 48 30869.5 49 30327.5 50 23892.5 51 20138.0 52 17277.5 53 16315.0 54 15713.0 55 14539.5 56 13182.5 57 12029.0 58 11175.0 59 10315.0 60 9382.0 61 8474.5 62 7103.5 63 5040.0 64 3251.5 65 2407.5 66 1800.5 67 1300.5 68 919.0 69 605.5 70 466.0 71 370.5 72 278.0 73 255.5 74 198.5 75 123.0 76 100.5 77 68.5 78 41.0 79 28.5 80 17.5 81 13.0 82 8.5 83 5.5 84 3.5 85 3.0 86 2.0 87 2.5 88 3.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24765958713479885 2 0.22459033472741075 3 0.21491613210495764 4 0.22101832145142805 5 0.2195299825864353 6 0.24259923499382338 7 0.2452782449508104 8 0.23262736459837177 9 0.22860884966289124 10-11 0.2361249609311048 12-13 0.2374644659095983 14-15 0.2228043280894194 16-17 0.2361249609311048 18-19 0.22667400913840063 20-21 0.21990206730268347 22-23 0.21625563708345114 24-25 0.2114185357722246 26-27 0.2218369078271741 28-29 0.23694354730685085 30-31 0.23805980145559538 32-33 0.23776213368259685 34-35 0.24483174329131258 36-37 0.21833931149444102 38-39 0.21766955900519427 40-41 0.21379987795621308 42-43 0.21886023009718855 44-45 0.23925047254758966 46-47 0.24550149578055933 48-49 0.24818050573754635 50-51 0.25048743097828513 52-53 0.2698358362231913 54-55 0.24892467517004271 56-57 0.21610680319695189 58-59 0.19869323847653636 60-61 0.19713048266829392 62-63 0.2048698447662564 64-65 0.19608864546279894 66-67 0.19876765541978597 68-69 0.20256291952551758 70-71 0.2015210823200226 72-73 0.20494426170950603 74-75 0.20568843114200241 76-77 0.22079507062167913 78-79 0.2136510440697138 80-81 0.2075488547232434 82-83 0.20412567533375997 84-85 0.2048698447662564 86-87 0.21149295271547427 88-89 0.20918602747473541 90-91 0.21990206730268347 92-93 0.20851627498548872 94-95 0.20412567533375997 96-97 0.21536263376445547 98-99 0.22310199586241794 100-101 0.19742815044129247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 671890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.13137782829283 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24633530779481 41.573356049177356 2 12.100931901092808 12.858783698228077 3 3.9065996610500657 6.226890678453953 4 1.8139840285681708 3.8551788318537685 5 0.9994578173199228 2.6551285457732847 6 0.598568444252651 1.908165970060862 7 0.3816283937154617 1.4193509663550483 8 0.2990586489507538 1.2711518456177031 9 0.2302979868359456 1.1012444416508254 >10 1.2676890641692342 12.454367602494859 >50 0.09691041591743146 3.51038623605115 >100 0.05097596229933225 4.860875415702991 >500 0.00476149098399494 1.8789313310862583 >1k 0.0022407016395270306 2.359304397516359 >5k 5.601754098817577E-4 2.0668839899775895 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8105 1.2062986500766493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5784 0.8608551995118249 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4372 0.6507017517748441 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2876 0.428046257571924 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2046 0.30451413177752307 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1664 0.24765958713479885 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1380 0.20539076336900386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1305 0.19422822188155797 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1175 0.17487981663665184 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1036 0.15419190641325217 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 986 0.14675021208828826 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 967 0.14392236824480198 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 931 0.13856434833082798 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 904 0.13454583339534745 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 810 0.12055544806441532 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 787 0.11713226867493191 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 759 0.11296491985295211 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 726 0.10805340159847594 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 714 0.1062673949604846 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 711 0.10582089330098676 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 684 0.10180237836550626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.4883388649927816E-4 0.0 0.0 0.0 0.0 3 1.4883388649927816E-4 0.0 0.0 0.0 0.0 4 1.4883388649927816E-4 0.0 0.0 0.0 0.0 5 1.4883388649927816E-4 0.0 0.0 0.0 0.0 6 2.976677729985563E-4 0.0 0.0 0.0 0.0 7 2.976677729985563E-4 0.0 0.0 0.0 0.0 8 2.976677729985563E-4 0.0 0.0 0.0 0.0 9 2.976677729985563E-4 0.0 0.0 0.0 0.0 10-11 2.976677729985563E-4 0.0 0.0 0.0 0.0 12-13 2.976677729985563E-4 0.0 0.0 0.0 0.0 14-15 2.976677729985563E-4 0.0 0.0 0.0 0.0 16-17 2.976677729985563E-4 0.0 0.0 0.0 0.0 18-19 2.976677729985563E-4 0.0 0.0 0.0 0.0 20-21 2.976677729985563E-4 0.0 0.0 0.0 0.0 22-23 2.976677729985563E-4 0.0 0.0 1.4883388649927816E-4 0.0 24-25 4.4650165949783446E-4 0.0 0.0 1.4883388649927816E-4 0.0 26-27 4.4650165949783446E-4 0.0 0.0 5.209186027474736E-4 0.0 28-29 4.4650165949783446E-4 0.0 0.0 0.0016371727514920597 0.0 30-31 4.4650165949783446E-4 0.0 0.0 0.0036464302192323146 0.0 32-33 4.4650165949783446E-4 0.0 0.0 0.006399857119468961 0.0 34-35 5.209186027474736E-4 0.0 0.0 0.009376534849454523 0.0 36-37 7.441694324963907E-4 0.0 0.0 0.012204378692940807 0.0 38-39 8.930033189956689E-4 0.0 0.0 0.015776391968923484 0.0 40-41 8.930033189956689E-4 0.0 0.0 0.020018157734152914 0.0 42-43 8.930033189956689E-4 0.0 0.0 0.027236601229367902 0.0 44-45 8.930033189956689E-4 0.0 0.0 0.043608328744288494 0.0 46-47 9.67420262245308E-4 0.0 0.0 0.07932846150411527 0.0 48-49 0.0010418372054949471 0.0 0.0 0.10768131688222773 0.0 50-51 0.0010418372054949471 0.0 0.0 0.15061989313726948 0.0 52-53 0.0010418372054949471 0.0 0.0 0.19698164878179464 0.0 54-55 0.0010418372054949471 0.0 0.0 0.2407388114125824 0.0 56-57 0.0010418372054949471 0.0 0.0 0.2961050171903139 0.0 58-59 0.0010418372054949471 0.0 0.0 0.38019616306240606 0.0 60-61 0.0011162541487445862 0.0 0.0 0.4904076560151215 0.0 62-63 0.0011906710919942253 0.0 0.0 0.6277069163107056 0.0 64-65 0.0011906710919942253 0.0 0.0 0.7426066766881484 0.0 66-67 0.0013395049784935035 0.0 0.0 0.9103424667728348 0.0 68-69 0.0013395049784935035 0.0 0.0 1.1085147866466238 0.0 70-71 0.0013395049784935035 0.0 0.0 1.2767714953340576 0.0 72-73 0.0013395049784935035 0.0 0.0 1.5141615443004064 0.0 74-75 0.0013395049784935035 0.0 0.0 1.7309380999866049 0.0 76-77 0.0013395049784935035 0.0 0.0 1.9353614430933634 0.0 78-79 0.0014139219217431426 0.0 0.0 2.1817559421929182 0.0 80-81 0.0017860066379913378 0.0 0.0 2.4454151721263897 0.0 82-83 0.0017860066379913378 0.0 0.0 2.762729018142851 0.0 84-85 0.0017860066379913378 0.0 0.0 3.1676316063641368 0.0 86-87 0.0017860066379913378 0.0 0.0 3.642039619580586 0.0 88-89 0.0017860066379913378 0.0 0.0 4.191087826876423 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGTA 55 0.0025762604 34.552567 9 CGTCACT 130 3.02427E-7 29.241148 1 TACCTGG 395 0.0 26.459166 2 GTACATG 7785 0.0 25.81841 1 GTATCAA 11145 0.0 25.666355 1 TACATGG 7865 0.0 25.308468 2 GAGTACT 5450 0.0 24.882578 12-13 AGTACTT 5550 0.0 24.819372 12-13 ACATGGG 7880 0.0 24.299324 3 ATGGGTA 240 2.2737368E-10 23.760206 5 CATGGGG 1680 0.0 22.911627 4 GTACTTT 5930 0.0 22.905962 14-15 GTATTGC 125 2.2491273E-4 22.808094 1 ATGGGAG 1940 0.0 22.780405 5 TACTTTT 6270 0.0 22.118338 14-15 ATGGGAT 1945 0.0 21.98888 5 GTCTAGG 130 2.9326888E-4 21.93086 1 CATGGGA 6160 0.0 21.754475 4 ATCAACG 13230 0.0 21.691643 3 GTACTAG 110 0.002870209 21.598576 1 >>END_MODULE